LRRK2 is a member of the leucine-rich repeat kinase family. It role is yet unknown but it may play a role in the phoshorylation of proteins central to parkinson diseases. LRRK2 contains an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a mLK-like domain and a WD40 domain. LRRK2 is present in the cytoplasm but also associates with the mitochondrial outer membrane. Defects in LRRK2 are the cause of Parkinson disease 8 (PARK8). Parkinson disease is characterised by bradykinesia, resting tremor, muscular rigidity and postural instability, as well as by a clinically significant response to treatment with levodopa. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain. PARK8 is an autosomal-dominant late-onset parkinsonism, characterized by onset from 50 to 65 years, with slow progression and relatively benign course.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human
Immunogen:
A synthetic peptide (LKRKRKILSSDDSLRSS; aa 946-962) as part of human LRRK2 protein conjugated to the Blue Carrier Protein has been used as the immunogen.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A dilution range of 1:500-1:10000 is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
LRRK2 is a member of the leucine-rich repeat kinase family. Its role is yet unknown but it may play a role in the phoshorylation of proteins central to parkinson diseases. LRRK2 contains an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a mLK-like domain and a WD40 domain. LRRK2 is present in the cytoplasm but also associates with the mitochondrial outer membrane. Defects in LRRK2 are the cause of Parkinson disease 8 (PARK8). Parkinson disease is characterised by bradykinesia, resting tremor, muscular rigidity and postural instability, as well as by a clinically significant response to treatment with levodopa. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain. PARK8 is an autosomal-dominant late-onset parkinsonism, characterized by onset from 50 to 65 years, with slow progression and relatively benign course.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
A synthetic peptide (CLKRKRKILSSDDSLRSS) corresponding to amino acids 946 - 962 of the human LRRK2 protein conjugated to diptheria toxin was used as the immunogen.
Applications:
IHC-Frozen
Antibody Isotype:
IgG
Application Details:
IHC. A dilution range of 1:500 to 1:1000 is recommended. Other applications have not yet been tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Zimprich A, et al. Neuron 44:601-607(2004). Ota T, et al. Nat. Genet. 36:40-45(2004).
Specificity:
Specificity was demonstrated by immunohistochemistry. The antibody stains positive tangles in inferior temporal cortex of human brain affected by Alzheimer's disease. This antiserum has been successfully tested in human. Other species have not yet been tested.
Phospho-LRRK2 (Ser910) Antibody detects endogenous levels of LRRK2 only when phosphorylated at Ser910.
Blocking Peptide:
A corresponding control peptide is available for this antibody (please email tech@nktscientific.com for details)
Conjugate:
Unconjugated
Uniprot:
Q5S007
Gene:
LRRK2
Format:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Phospho-LRRK2 (Ser910) Antibody detects endogenous levels of LRRK2 only when phosphorylated at Ser910.
Blocking Peptide:
A corresponding control peptide is available for this antibody (please email tech@nktscientific.com for details)
Conjugate:
Unconjugated
Uniprot:
Q5S007
Gene:
LRRK2
Format:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Phospho-LRRK2 (Ser935) Antibody detects endogenous levels of LRRK2 only when phosphorylated at Ser935.
Blocking Peptide:
A corresponding control peptide is available for this antibody (please email tech@nktscientific.com for details)
Conjugate:
Unconjugated
Uniprot:
Q5S007
Gene:
LRRK2
Format:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Phospho-LRRK2 (Ser935) Antibody detects endogenous levels of LRRK2 only when phosphorylated at Ser935.
Blocking Peptide:
A corresponding control peptide is available for this antibody (please email tech@nktscientific.com for details)
Conjugate:
Unconjugated
Uniprot:
Q5S007
Gene:
LRRK2
Format:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
LRRK2 Antibody detects endogenous levels of total LRRK2.
Blocking Peptide:
A corresponding control peptide is available for this antibody (please email tech@nktscientific.com for details)
Conjugate:
Unconjugated
Uniprot:
Q5S007
Gene:
LRRK2
Format:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
LRRK2 Antibody detects endogenous levels of total LRRK2.
Blocking Peptide:
A corresponding control peptide is available for this antibody (please email tech@nktscientific.com for details)
Conjugate:
Unconjugated
Uniprot:
Q5S007
Gene:
LRRK2
Format:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
LRRK2 Antibody detects endogenous levels of total LRRK2.
Blocking Peptide:
A corresponding control peptide is available for this antibody (please email tech@nktscientific.com for details)
Conjugate:
Unconjugated
Uniprot:
Q5S007
Gene:
LRRK2
Format:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
LRRK2 Antibody detects endogenous levels of total LRRK2.
Blocking Peptide:
A corresponding control peptide is available for this antibody (please email tech@nktscientific.com for details)
Conjugate:
Unconjugated
Uniprot:
Q5S007
Gene:
LRRK2
Format:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
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