NEXTFLEX® UDI Barcoded Adapters (1-1,536) for Ultra High-Throughput Multiplexing
Sets of 384 UDI barcodes for multiplexing NGS libraries compatible with Illumina® Up to 1,536 (10-nt) adapters available for purchase off-the-shelf Every lot is functionally validated and tested for index purity by sequencing
Highest Sample Multiplexing Options for Illumina® Sequencing The superior design of the NEXTFLEX® Unique Dual Index barcodes allows a broad range of multiplexing users can multiplex anywhere between two samples and 1,536 samples in a single sequencing run. The wide range of multiplexing options enables barcode rotation (avoiding frequent use of the same barcode set) to mitigate run-to-run sample carryover and crosstalk. NEXTFLEX® Unique Dual Index barcodes are compatible with NEXTFLEX® library prep kits designed for Illumina® paired-end and single-read sequencing, or other workflows for Illumina® sequencing that involve the ligation of adapters to adenylated fragments.
Highest Level of Data Integrity The NEXTFLEX® Unique Dual Index barcodes utilize two unique indexes on each UDI adapter, with a hamming distance of at least three throughout the entire series to permit error correction. Each NEXTFLEX® Unique Dual Index barcode comprises the entire flow cell binding site, sequencing primer binding site and index sequences, thereby making PCR enrichment optional. Patterned flow cells, such as those found on the Illumina® NovaSeq®, HiSeq® 3000/4000, and HiSeq® X platforms, have been known to suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices improves data integrity by correctly removing mis-assigned reads. Every lot is functionally validated and tested for index purity by sequencing.
KIT CONTENTS
NEXTFLEX® Unique Dual Index Barcodes* (25 µM) NEXTFLEX® Primer Mix** (50 µM)
The UDI barcodes are oriented column-wise on each plate (1-8, 9-16, etc).
* These Unique Dual Index barcodes are supplied in duplex form. Do not heat the adapters above room temperature.
** The Primer Mix 2.0 is only intended for use with the NEXTFLEX® Rapid XP DNA-Seq kit and the NEXTFLEX® Rapid DNA-Seq kit 2.0; if the primer mix is to be used with any other library kits of choice, additional dilutions may be required. For additional guidance, please contact us.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
NEXTFLEX® UDI Barcoded Adapters (1-1,536) for Ultra High-Throughput Multiplexing
Sets of 384 UDI barcodes for multiplexing NGS libraries compatible with Illumina® Up to 1,536 (10-nt) adapters available for purchase off-the-shelf Every lot is functionally validated and tested for index purity by sequencing
Highest Sample Multiplexing Options for Illumina® Sequencing The superior design of the NEXTFLEX® Unique Dual Index barcodes allows a broad range of multiplexing users can multiplex anywhere between two samples and 1,536 samples in a single sequencing run. The wide range of multiplexing options enables barcode rotation (avoiding frequent use of the same barcode set) to mitigate run-to-run sample carryover and crosstalk. NEXTFLEX® Unique Dual Index barcodes are compatible with NEXTFLEX® library prep kits designed for Illumina® paired-end and single-read sequencing, or other workflows for Illumina® sequencing that involve the ligation of adapters to adenylated fragments.
Highest Level of Data Integrity The NEXTFLEX® Unique Dual Index barcodes utilize two unique indexes on each UDI adapter, with a hamming distance of at least three throughout the entire series to permit error correction. Each NEXTFLEX® Unique Dual Index barcode comprises the entire flow cell binding site, sequencing primer binding site and index sequences, thereby making PCR enrichment optional. Patterned flow cells, such as those found on the Illumina® NovaSeq®, HiSeq® 3000/4000, and HiSeq® X platforms, have been known to suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices improves data integrity by correctly removing mis-assigned reads. Every lot is functionally validated and tested for index purity by sequencing.
KIT CONTENTS
NEXTFLEX® Unique Dual Index Barcodes* (25 µM) NEXTFLEX® Primer Mix** (50 µM)
The UDI barcodes are oriented column-wise on each plate (1-8, 9-16, etc).
* These Unique Dual Index barcodes are supplied in duplex form. Do not heat the adapters above room temperature.
** The Primer Mix 2.0 is only intended for use with the NEXTFLEX® Rapid XP DNA-Seq kit and the NEXTFLEX® Rapid DNA-Seq kit 2.0; if the primer mix is to be used with any other library kits of choice, additional dilutions may be required. For additional guidance, please contact us.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
NEXTFLEX® UDI Barcoded Adapters (1-1,536) for Ultra High-Throughput Multiplexing
Sets of 384 UDI barcodes for multiplexing NGS libraries compatible with Illumina® Up to 1,536 (10-nt) adapters available for purchase off-the-shelf Every lot is functionally validated and tested for index purity by sequencing
Highest Sample Multiplexing Options for Illumina® Sequencing The superior design of the NEXTFLEX® Unique Dual Index barcodes allows a broad range of multiplexing users can multiplex anywhere between two samples and 1,536 samples in a single sequencing run. The wide range of multiplexing options enables barcode rotation (avoiding frequent use of the same barcode set) to mitigate run-to-run sample carryover and crosstalk. NEXTFLEX® Unique Dual Index barcodes are compatible with NEXTFLEX® library prep kits designed for Illumina® paired-end and single-read sequencing, or other workflows for Illumina® sequencing that involve the ligation of adapters to adenylated fragments.
Highest Level of Data Integrity The NEXTFLEX® Unique Dual Index barcodes utilize two unique indexes on each UDI adapter, with a hamming distance of at least three throughout the entire series to permit error correction. Each NEXTFLEX® Unique Dual Index barcode comprises the entire flow cell binding site, sequencing primer binding site and index sequences, thereby making PCR enrichment optional. Patterned flow cells, such as those found on the Illumina® NovaSeq®, HiSeq® 3000/4000, and HiSeq® X platforms, have been known to suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices improves data integrity by correctly removing mis-assigned reads. Every lot is functionally validated and tested for index purity by sequencing.
KIT CONTENTS
NEXTFLEX® Unique Dual Index Barcodes* (25 µM) NEXTFLEX® Primer Mix** (50 µM)
The UDI barcodes are oriented column-wise on each plate (1-8, 9-16, etc).
* These Unique Dual Index barcodes are supplied in duplex form. Do not heat the adapters above room temperature.
** The Primer Mix 2.0 is only intended for use with the NEXTFLEX® Rapid XP DNA-Seq kit and the NEXTFLEX® Rapid DNA-Seq kit 2.0; if the primer mix is to be used with any other library kits of choice, additional dilutions may be required. For additional guidance, please contact us.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
NEXTFLEX® UDI Barcoded Adapters (1-1,536) for Ultra High-Throughput Multiplexing
Sets of 384 UDI barcodes for multiplexing NGS libraries compatible with Illumina® Up to 1,536 (10-nt) adapters available for purchase off-the-shelf Every lot is functionally validated and tested for index purity by sequencing
Highest Sample Multiplexing Options for Illumina® Sequencing The superior design of the NEXTFLEX® Unique Dual Index barcodes allows a broad range of multiplexing users can multiplex anywhere between two samples and 1,536 samples in a single sequencing run. The wide range of multiplexing options enables barcode rotation (avoiding frequent use of the same barcode set) to mitigate run-to-run sample carryover and crosstalk. NEXTFLEX® Unique Dual Index barcodes are compatible with NEXTFLEX® library prep kits designed for Illumina® paired-end and single-read sequencing, or other workflows for Illumina® sequencing that involve the ligation of adapters to adenylated fragments.
Highest Level of Data Integrity The NEXTFLEX® Unique Dual Index barcodes utilize two unique indexes on each UDI adapter, with a hamming distance of at least three throughout the entire series to permit error correction. Each NEXTFLEX® Unique Dual Index barcode comprises the entire flow cell binding site, sequencing primer binding site and index sequences, thereby making PCR enrichment optional. Patterned flow cells, such as those found on the Illumina® NovaSeq®, HiSeq® 3000/4000, and HiSeq® X platforms, have been known to suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices improves data integrity by correctly removing mis-assigned reads. Every lot is functionally validated and tested for index purity by sequencing.
KIT CONTENTS
NEXTFLEX® Unique Dual Index Barcodes* (25 µM) NEXTFLEX® Primer Mix** (50 µM)
The UDI barcodes are oriented column-wise on each plate (1-8, 9-16, etc).
* These Unique Dual Index barcodes are supplied in duplex form. Do not heat the adapters above room temperature.
** The Primer Mix 2.0 is only intended for use with the NEXTFLEX® Rapid XP DNA-Seq kit and the NEXTFLEX® Rapid DNA-Seq kit 2.0; if the primer mix is to be used with any other library kits of choice, additional dilutions may be required. For additional guidance, please contact us.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
NEXTFLEX® UDI Barcoded Adapters (1-1,536) for Ultra High-Throughput Multiplexing
Sets of 384 UDI barcodes for multiplexing NGS libraries compatible with Illumina® Up to 1,536 (10-nt) adapters available for purchase off-the-shelf Every lot is functionally validated and tested for index purity by sequencing
Highest Sample Multiplexing Options for Illumina® Sequencing The superior design of the NEXTFLEX® Unique Dual Index barcodes allows a broad range of multiplexing users can multiplex anywhere between two samples and 1,536 samples in a single sequencing run. The wide range of multiplexing options enables barcode rotation (avoiding frequent use of the same barcode set) to mitigate run-to-run sample carryover and crosstalk. NEXTFLEX® Unique Dual Index barcodes are compatible with NEXTFLEX® library prep kits designed for Illumina® paired-end and single-read sequencing, or other workflows for Illumina® sequencing that involve the ligation of adapters to adenylated fragments.
Highest Level of Data Integrity The NEXTFLEX® Unique Dual Index barcodes utilize two unique indexes on each UDI adapter, with a hamming distance of at least three throughout the entire series to permit error correction. Each NEXTFLEX® Unique Dual Index barcode comprises the entire flow cell binding site, sequencing primer binding site and index sequences, thereby making PCR enrichment optional. Patterned flow cells, such as those found on the Illumina® NovaSeq®, HiSeq® 3000/4000, and HiSeq® X platforms, have been known to suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices improves data integrity by correctly removing mis-assigned reads. Every lot is functionally validated and tested for index purity by sequencing.
KIT CONTENTS
NEXTFLEX® Unique Dual Index Barcodes* (25 µM) NEXTFLEX® Primer Mix** (50 µM)
The UDI barcodes are oriented column-wise on each plate (1-8, 9-16, etc).
* These Unique Dual Index barcodes are supplied in duplex form. Do not heat the adapters above room temperature.
** The Primer Mix 2.0 is only intended for use with the NEXTFLEX® Rapid XP DNA-Seq kit and the NEXTFLEX® Rapid DNA-Seq kit 2.0; if the primer mix is to be used with any other library kits of choice, additional dilutions may be required. For additional guidance, please contact us.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
The NEXTflex Rapid XP DNA-Seq kit combines enzymatic fragmentation with end-repair and A-tailing in one reaction to create a highly efficient first step in library generation, which is followed by ligation and PCR (optional for PCR-free workflows). This effective one-tube workflow produces DNA-seq libraries with consistent library size, high yield, low GC-bias, and high coverage.
The kit includes all the required reagents for fragmentation, library prep, and magnetic bead-based cleanup, and it is compatible with your NEXTflex barcodes of choice. This DNA-seq library prep kit is highly flexible in terms of sample requirements, accommodating a range of sample types and sample input amounts from 1 ng to 1 µg to quickly and efficiently generate high-quality libraries for Illumina sequencing.
For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
[BENEFITS] Reliable: Consistent yields and high-quality sequencing metrics Convenient: Kit includes fragmentation enzyme and cleanup/size selection beads Streamlined: Single step for fragmentation, end-repair, and adenylation with minimal hands-on time Flexible: Chemistry optimized with NEXTflex color-balanced barcodes that allow a wide range of multiplexing (2 up to 384 samples in one run Automation-friendly workflow is compatible with liquid handlers Flexible barcode options Efficient: Automated on the PerkinElmer Sciclone G3 and Zephyr G3 NGS workstation
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Flexible Multiplexing Options We offer a broad range of NEXTflex adapters adapters that improve multiplexing capabilities and offer a solution to flexible experimental setups for both low-level and high-level multiplexing needs. The NEXTflex Rapid XP DNA-seq kit is compatible with NEXTflex DNA Barcodes, NEXTflex-96 DNA Barcodes, NEXTflex ChIP-Seq Barcodes, and NEXTflex-96 ChIP-Seq Barcodes, and and NEXTflex-HT barcodes. For those high throughput users interested in dual index barcodes, the reagents are also compatible with the NEXTflex Unique Dual Index Barcodes and the NEXTflex Dual-Indexed barcodes.
The NEXTflex Rapid XP DNA-Seq Kit contains enough material to prepare 48 DNA samples for Illumina compatible sequencing. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.
The NEXTflex Rapid XP DNA-Seq kit combines enzymatic fragmentation with end-repair and A-tailing in one reaction to create a highly efficient first step in library generation, which is followed by ligation and PCR (optional for PCR-free workflows). This effective one-tube workflow produces DNA-seq libraries with consistent library size, high yield, low GC-bias, and high coverage.
The kit includes all the required reagents for fragmentation, library prep, and magnetic bead-based cleanup, and it is compatible with your NEXTflex barcodes of choice. This DNA-seq library prep kit is highly flexible in terms of sample requirements, accommodating a range of sample types and sample input amounts from 1 ng to 1 µg to quickly and efficiently generate high-quality libraries for Illumina sequencing.
For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
[BENEFITS] Reliable: Consistent yields and high-quality sequencing metrics Convenient: Kit includes fragmentation enzyme and cleanup/size selection beads Streamlined: Single step for fragmentation, end-repair, and adenylation with minimal hands-on time Flexible: Chemistry optimized with NEXTflex color-balanced barcodes that allow a wide range of multiplexing (2 up to 384 samples in one run Automation-friendly workflow is compatible with liquid handlers Flexible barcode options Efficient: Automated on the PerkinElmer Sciclone G3 and Zephyr G3 NGS workstation
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Flexible Multiplexing Options We offer a broad range of NEXTflex adapters adapters that improve multiplexing capabilities and offer a solution to flexible experimental setups for both low-level and high-level multiplexing needs. The NEXTflex Rapid XP DNA-seq kit is compatible with NEXTflex DNA Barcodes, NEXTflex-96 DNA Barcodes, NEXTflex ChIP-Seq Barcodes, and NEXTflex-96 ChIP-Seq Barcodes, and and NEXTflex-HT barcodes. For those high throughput users interested in dual index barcodes, the reagents are also compatible with the NEXTflex Unique Dual Index Barcodes and the NEXTflex Dual-Indexed barcodes.
The NEXTflex Rapid XP DNA-Seq Kit contains enough material to prepare 48 DNA samples for Illumina compatible sequencing. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.
The NEXTflex Rapid XP DNA-Seq kit combines enzymatic fragmentation with end-repair and A-tailing in one reaction to create a highly efficient first step in library generation, which is followed by ligation and PCR (optional for PCR-free workflows). This effective one-tube workflow produces DNA-seq libraries with consistent library size, high yield, low GC-bias, and high coverage.
The kit includes all the required reagents for fragmentation, library prep, and magnetic bead-based cleanup, and it is compatible with your NEXTflex barcodes of choice. This DNA-seq library prep kit is highly flexible in terms of sample requirements, accommodating a range of sample types and sample input amounts from 1 ng to 1 µg to quickly and efficiently generate high-quality libraries for Illumina sequencing.
For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
[BENEFITS] Reliable: Consistent yields and high-quality sequencing metrics Convenient: Kit includes fragmentation enzyme and cleanup/size selection beads Streamlined: Single step for fragmentation, end-repair, and adenylation with minimal hands-on time Flexible: Chemistry optimized with NEXTflex color-balanced barcodes that allow a wide range of multiplexing (2 up to 384 samples in one run Automation-friendly workflow is compatible with liquid handlers Flexible barcode options Efficient: Automated on the PerkinElmer Sciclone G3 and Zephyr G3 NGS workstation
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Flexible Multiplexing Options We offer a broad range of NEXTflex adapters adapters that improve multiplexing capabilities and offer a solution to flexible experimental setups for both low-level and high-level multiplexing needs. The NEXTflex Rapid XP DNA-seq kit is compatible with NEXTflex DNA Barcodes, NEXTflex-96 DNA Barcodes, NEXTflex ChIP-Seq Barcodes, and NEXTflex-96 ChIP-Seq Barcodes, and and NEXTflex-HT barcodes. For those high throughput users interested in dual index barcodes, the reagents are also compatible with the NEXTflex Unique Dual Index Barcodes and the NEXTflex Dual-Indexed barcodes.
The NEXTflex Rapid XP DNA-Seq Kit contains enough material to prepare 48 DNA samples for Illumina compatible sequencing. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.
The NEXTflex Rapid XP DNA-Seq kit combines enzymatic fragmentation with end-repair and A-tailing in one reaction to create a highly efficient first step in library generation, which is followed by ligation and PCR (optional for PCR-free workflows). This effective one-tube workflow produces DNA-seq libraries with consistent library size, high yield, low GC-bias, and high coverage.
The kit includes all the required reagents for fragmentation, library prep, and magnetic bead-based cleanup, and it is compatible with your NEXTflex barcodes of choice. This DNA-seq library prep kit is highly flexible in terms of sample requirements, accommodating a range of sample types and sample input amounts from 1 ng to 1 µg to quickly and efficiently generate high-quality libraries for Illumina sequencing.
For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
[BENEFITS] Reliable: Consistent yields and high-quality sequencing metrics Convenient: Kit includes fragmentation enzyme and cleanup/size selection beads Streamlined: Single step for fragmentation, end-repair, and adenylation with minimal hands-on time Flexible: Chemistry optimized with NEXTflex color-balanced barcodes that allow a wide range of multiplexing (2 up to 384 samples in one run Automation-friendly workflow is compatible with liquid handlers Flexible barcode options Efficient: Automated on the PerkinElmer Sciclone G3 and Zephyr G3 NGS workstation
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Flexible Multiplexing Options We offer a broad range of NEXTflex adapters adapters that improve multiplexing capabilities and offer a solution to flexible experimental setups for both low-level and high-level multiplexing needs. The NEXTflex Rapid XP DNA-seq kit is compatible with NEXTflex DNA Barcodes, NEXTflex-96 DNA Barcodes, NEXTflex ChIP-Seq Barcodes, and NEXTflex-96 ChIP-Seq Barcodes, and and NEXTflex-HT barcodes. For those high throughput users interested in dual index barcodes, the reagents are also compatible with the NEXTflex Unique Dual Index Barcodes and the NEXTflex Dual-Indexed barcodes.
The NEXTflex Rapid XP DNA-Seq Kit contains enough material to prepare 48 DNA samples for Illumina compatible sequencing. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.
These kits ship on dry ice.
NGS Applications:
Rapid DNA-Sequncing
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