Product Search Results : SKT-133


Product - please click for more details Brand Cat No Size Price (£)

StressMarq SKT-133-96 96 well £329.00
Please sign in or sign up to order this product online.
Product Detail: Fluorometric detection of purified 20S proteasome activity
Background Info: Proteasomes are non-lysosomal proteolytic complexes localised primarily in the cytoplasm and in the nucleus of eukaryotic cells. They are responsible for the ubiquitin-mediated degradation of short half-life proteins and peptides that are involved in essential cellular processes including cell-cycle regulation, apoptosis and transcriptional regulation, innate immunity and antigen processing, and in the removal of redundant or damaged proteins. As such protein degradation by the ubiquitin-proteasome pathway has a major regulatory function for proliferation activity and survival of both normal and malignant cells, and its dysfunction has been implicated in a wide range of other disease processes including neurodegenerative, cardiovascular and metabolic disorders.
The 26S proteasome structure is composed of a 20S proteasome catalytic core complex and one or two 19S (PA700) regulatory subcomplexes. The 20S core comprises two copies of 14 subunits (7 alpha subunits and 7 beta subunits) arranged in a ?7?7?7?7 cylindrical array. Proteolytic activities are determined by the ?1 (caspase-like), ?2 (trypsin-like) and ?5 (chymotrypsin-like) subunits, access to which is guarded by the ?-subunits. The 19S regulatory unit consists of six ATPase and at least ten non-ATPase subunits that are required for ubiquitinated protein binding, deubiquitination, substrate unfolding and translocation to the 20S catalytic core.
Varying catalytic subunit composition (?1, ?1i; ?2, ?2i; ?5, ?5i) results in a variety of possible subtypes from full constitutive proteasome (?1, ?2, ?5) through mixed populations to full inducible / immunoproteasome (?1i, ?2i, ?5i). Alternative regulatory complexes such as the PA200 and 11S proteasome activators confer different substrate specificities and activity compared to the 19S regulator.
Product Type: Assay Kits
Storage Temp: -20ºC
Species Reactivity: ALL
Application Details: Activity kit used to screen potential proteasome inhibitors and activators, and to measure and quantify 20S proteasome activity in continuous kinetic and end-point assays.
Target: 20S Proteasome
Research Area(s): Cancer | Apoptosis | Cell Signaling | Post-translational Modifications | Neuroscience | Neurodegeneration | Cardiovascular System
Alternative Name(s): Chymotrypsin-like (?5) Activity Kit
Category: Activity Kits
Species Reactivity Full Name: Species Independent
References: 1. Saeki, Y. & Tanaka, K. Methods in molecular biology (Clifton, N.J.) 832, 315–37 (2012).
2. Frankland-Searby, S. & Bhaumik, S. R. Biochimica et biophysica acta 1825, 64–76 (2012).
3. Basler, M., Kirk, C. J. & Groettrup, M. Current opinion in immunology 1–7 (2012).
4. Löw, P. General and comparative endocrinology 172, 39–43 (2011).
5. Dennissen, F. J. a, Kholod, N. & Van Leeuwen, F. W. Progress in neurobiology 96, 190–207 (2012).
6. Geng, F., Wenzel, S. & Tansey, W. P. Annual review of biochemistry 81, 177–201 (2012).
Country of Origin: Canada
Assay Type: Fluorometric Assay
Assay Type Abbreviation: Continuous Kinetic Assay
Platform: Enzyme Activity
Utility: Microplate
Assay Overview: Activity kit used to screen potential proteasome inhibitors and activators, and to measure and quantify 20S proteasome activity in continuous kinetic and end-point assays.
Sample Type(s): Purified Proteins
Number of Samples: 40 samples in duplicate using the plate format, or 100x 50µL micro-cuvette based assays
Incubation Time: 15-30 minutes