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Product Search Results: TR-601-ER1

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Product Search Results: TR-601-ER1

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Biosensis TR-601-ER1 1.8 mg £464.00
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Product Detail: TR-601-ER1 is a cell-permeant stain for the endoplasmic reticulum. TR-601-ER1 passively diffuses across the plasma membrane into the cell, stains at low concentrations and has minimal cytotoxic effects. It can be used as a real-time imaging reagent which can be imaged within minutes of addition and has minimal photobleaching. TR-601-ER1 is easily washed from cells, and therefore is ideal for protocols which require intermittent monitoring of endoplasmic reticulum structures. This tracing reagent can be used on live and fixed samples and is compatible with other dyes. TR-601-ER1 has a large Stokes Shift, shows excellent resistance to photobleaching and demonstrates low cytotoxicity. TR-601-ER1 is manufactured by ReZolve Scientific.
Background Info: TR-601-ER1 is a cell-permeant stain for the endoplasmic reticulum. TR-601-ER1 passively diffuses across the plasma membrane into the cell, stains at low concentrations and has minimal cytotoxic effects. It can be used as a real-time imaging reagent which can be imaged within minutes of addition and has minimal photobleaching. TR-601-ER1 is easily washed from cells, and therefore is ideal for protocols which require intermittent monitoring of endoplasmic reticulum structures. This tracing reagent can be used on live and fixed samples and is compatible with other dyes. TR-601-ER1 has a large Stokes Shift, shows excellent resistance to photobleaching and demonstrates low cytotoxicity. TR-601-ER1 is manufactured by ReZolve Scientific.
Product Type: Tracing/Staining Kit
Format: Dry, synthetic chemical powder
Storage Temp: Product is shipped at room temperature. Store lyophilised product at room temperature, properly sealed and protected from light. Stock solution can be stored at room temperature, properly sealed and protected from light. Working solutions should be used immediately and not stored for later use.
Species Reactivity: All eukaryotes
Applications: Fluorescent imaging
Data Sheet: PLEASE CLICK HERE TO DOWNLOAD
Application Details: Fluorescent imaging of the endoplasmic reticulum compartment.

Sample types: live cells, fixed cells (4% paraformaldehyde), tissue (frozen only)
Epifluorescence microscopy: YES
Confocal microscopy: YES
Multiphoton imaging: YES
Infrared spectroscopy: NO
Raman spectroscopy: NO
Ex/Em: 405 nm / 570 nm

Typical working dilution is 1:100 - 1:200 (50-100 µM) in appropriate buffer or cell culture medium. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Reconstitution: Spin vial briefly before opening. Reconstitute TR-601-ER1 with 300 µL of DMSO to obtain a 10 mM stock solution, mix thoroughly before use. Avoid PBS or cell culture medium for reconstitution.
Shelf Life: Lyophilised product is stable for 6 months at room temperature if protected from light. After reconstitution in DMSO, liquid 10 mM stock solution is stable for 6 months at room temperature.
Specificity: Cell penetration and localisation of TR-601-ER1 has been confirmed in a range of cell lines, including prostate cells (PNT2, PNT1a, LNCaP, 22RV1 and DU145), cardiomyocytes (H9c2) and neuronal cells (PC-12).