| Product Detail: |
The fluorescent dye Fluoro-Jade® B (FJB), like its more purified brother Fluoro-Jade® C (FJC), is an anionic fluorescein derivative useful for the histological staining of neurons undergoing degeneration. Fluoro-Jade® B differs from FJC in that it is a slightly less refined chemical formulation and thus it does not quite provide the same level of signal to noise or high resolution as FJC. Nonetheless FJB is still widely used and works very well as a marker of degenerating neurons and even glia (see Damjanac M et al., Brain Res. 2007;1128(1):40-9). FJB operates nearly identically in protocol to that of FJC, and Fluoro-Jade® B is compatible with several other labeling procedures including immunofluorescent and fluorescent Nissl techniques Fluoro-Jade B stains all degenerating neurons regardless of specific insult or mechanism of cell death. Fluoro-Jade B exhibits the greatest signal to background ratio, as well as the highest resolution. This translates to a stain of maximal contrast and affinity for degenerating neurons. This makes it ideal for localising not only degenerating nerve cell bodies but also distal dendrites, axons and terminals. The dye is highly resistant to fading and is compatible with virtually all histological processing and staining protocols. |
| Product Type: |
Tracing/Staining Kit |
| Format: |
Dry powder |
| Storage Temp: |
The powdered dye can be stored desiccated at room temperature in the dark. Storage in a desiccator is recommended as FJB is hydroscopic. The 0.01% stock solution will remain stable for 3 months when stored in a refrigerator, in the dark. The 0.0001-0.0004% working solution in 0.1% acetic acid should be used within 4 hours of preparation. Diluted FJB dye solutions are not stable and should not be stored. The other diluted solutions can be reused and stored for up to 48 hours if refrigerated and protected from light. Best results require freshly diluted solutions. |
| Species Reactivity: |
Human, Mouse, Rat |
| Applications: |
IH, Frozen; IH(P), Paraffin; IC; FC |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Application Details: |
Following our detailed protocol, Fluoro-Jade B labels degenerating neurons which are visualised with blue light excitation, while DAPI (not included) counter stains cell nuclei, visualised with ultra-violet illumination. The Fluoro-Jade B dye can be used on all kinds of preserved tissues, including fresh-frozen, paraformaldehyde or formalin fixed, and formalin fixed, paraffin-embedded tissues.
Full ready-to-dilute (RTD) versions of FJC are available as TR-100-FJ or a trial version (TR-100-FJT). There is no trial powder version available. The FJC dye stain is compatible with all common fixations, embedding, sectioning and staining procedures, although the use of slide- mounted, formalin fixed, freeze-cut tissue sections is most typical. The powder is shipped at ambient temperature, see storage instructions for details. |
| Alternative Names: |
FJB |
| Kit Components: |
30 mg Fluoro-Jade® B dry powder |
| Reconstitution: |
Dissolve Fluoro-Jade® B powder in distilled water (10 mg powder per in 100 mL water) to prepare a 0.01% stock solution, filter through a 0.45 µm membrane and store at 4C in the dark for up to 3 months. Discard if cloudy or precipitated. We recommend using aseptic techniques when handling the reagent to avoid bacterial growth and contamination. |
| Shelf Life: |
See Storage temp instructions for shelf life recommendations. |
| Specificity: |
Degenerating neurons, and neuronal degeneration. There is no specific staining in normal healthy brain. | Note: Some researchers under some conditions report blood vessel staining with Fluoro Jade. This may be because Fluoro Jade is an analogue of eosin (which stains blood cells). In general, good perfusion and preparation of the tissue should help prevent blood vessel staining but it may not be possible to eliminate it entirely. In our experience it is generally possible to distinguish neuronal from blood vessels staining by eye. |
| References: |
Huang YN, Yang LY, Greig NH, Wang YC, Lai CC, Wang JY (2018) "Neuroprotective effects of pifithrin-α against traumatic brain injury in the striatum through suppression of neuroinflammation, oxidative stress, autophagy, and apoptosis." Sci Rep. 8(1): 2368 Application: IF. Species: RatFreitas-Andrade M, She J, Bechberger J, Naus CC, Sin WC (2017) "Acute connexin43 temporal and spatial expression in response to ischemic stroke." J Cell Commun Signal. [Epub ahead of print] Application: IF. Species: MouseXie Z, Enkhjargal B, Wu L, Zhou K, Sun C, Hu X, Gospodarev V, Tang J, You C, Zhang JH (2017) "Exendin-4 attenuates neuronal death via GLP-1R/PI3K/Akt pathway in early brain injury after subarachnoid hemorrhage in rats." Neuropharmacology. 128:142-51 Application: IF. Species: RatKhan D (2017) "The Relationship between Astrocytes, Inflammation and Epileptogenesis." PhD Thesis. Application: IF. Species: MouseFan LF, He PY, Peng YC, Du QH, Ma YJ, Jin JX, Xu HZ, Li JR, Wang ZJ, Cao SL, Li T, Yan F, Gu C, Wang L, Chen G (2017) "Mdivi-1 ameliorates early brain injury after subarachnoid hemorrhage via the suppression of inflammation-related blood-brain barrier disruption and endoplasmic reticulum stress-based apoptosis." Free Radic Biol Med. [Epub ahead of print] Application: IF. Species: RatMoro C, Torres N, Arvanitakis K, Cullen K, Chabrol C, Agay D, Darlot F, Benabid AL, Mitrofanis J (2017) "No evidence for toxicity after long-term photobiomodulation in normal non-human primates." Exp Brain Res. [Epub ahead of print] Application: IF. Species: PrimateFan R, Enkhjargal B, Camara R, Yan F, Gong L, ShengtaoYao, Tang J, Chen Y, Zhang JH (2017) "Critical role of EphA4 in early brain injury after subarachnoid hemorrhage in rat." Exp Neurol. 296:41-8 Application: IF. Species: RatNakajima M, Nito C, Sowa K, Suda S, Nishiyama Y, Nakamura-Takahashi A, Nitahara-Kasahara Y, Imagawa K, Hirato T, Ueda M, Kimura K, Okada T (2017) "Mesenchymal Stem Cells Overexpressing Interleukin-10 Promote Neuroprotection in Experimental Acute Ischemic Stroke." Mol Ther Methods Clin Dev. 6:102-11 Application: IF. Species: HumanSuzuki K, Yamada K, Fukuhara Y, Tsuji A, Shibata K, Wakamatsu N (2017) "High-dose thiamine prevents brain lesions and prolongs survival of Slc19a3-deficient mice." PLoS One. 12(6):e0180279 Application: IF. Species: MouseKononenko NL, ClaÃen GA, Kuijpers M, Puchkov D, Maritzen T, Tempes A, Malik AR, Skalecka A, Bera S, Jaworski J, Haucke V (2017) "Retrograde transport of TrkB-containing autophagosomes via the adaptor AP-2 mediates neuronal complexity and prevents neurodegeneration." Nat Commun. 8:14819 Application: IF. Species: MouseLian YD, Chen ZX, Zhu KR, Sun SY, Zhu LP (2017) "Effect of equipotent doses of bupivacaine and ropivacaine in high‐fat diet fed neonatal rodent model." Braz J Anesthesiol. 67(2):131-8 Application: IF. Species: RatMamo JCL, Lam V, Giles C, Coulson SH, Fimognari N, Mooranian A, Al-Salami H, Takechi R (2017) "Antihypertensive agents do not prevent bloodbrain barrier dysfunction and cognitive deficits in dietary-induced obese mice." Int J Obes (Lond). [Epub ahead of print] Application: IF. Species: MouseIwasa K, Yamamoto S, Yagishita S, Maruyama K, Yoshikawa K (2017) "Excitotoxicity-induced prostaglandin D2 production induces sustained microglial activation and delayed neuronal death." J Lipid Res. 58(4):649-55 Application: IF. Species: RatXie Z, Huang L, Enkhjargal B, Reis C, Wan W, Tang J, Cheng Y, Zhang JH (2017) "Intranasal administration of recombinant Netrin-1 attenuates neuronal apoptosis by activating DCC/APPL-1/AKT signaling pathway after subarachnoid hemorrhage in rats." Neuropharmacology. http://dx.doi.org/10.1016/j.neuropharm.2017.03.025 [In press] Application: IF. Species: RatKatayama Y, Inaba T, Nito C, Suda S, Ueda M (2016) "Neuroprotective effects of clarithromycin against neuronal damage in cerebral ischemia and in cultured neuronal cells after oxygen-glucose deprivation." Life Sci. PMID:27825902 [Epub ahead of print] Application: IF. Species: RatYang LY, Greig NH, Huang YN, Hsieh TH, Tweedie D, Yu QS, Hoffer BJ, Luo Y, Kao YC, Wang JY (2016) "Post-traumatic administration of the p53 inactivator pifithrin-α oxygen analogue reduces hippocampal neuronal loss and improves cognitive deficits after experimental traumatic brain injury." Neurobiol Dis. PMID:27553877 [Epub ahead of print] Application: IF. Species: RatIzumida H, Takagi H, Fujisawa H, Iwata N, Nakashima K, Takeuchi S, Iwama S, Namba T, Komatu Y, Kaibuchi K, Oiso Y, Arima H, Sugimur Y (2016) "NMDA receptor antagonist prevents cell death in the hippocampal dentate gyrus induced by hyponatremia accompanying adrenal insufficiency in rats." Exp Neurol. PMID:27527984 [Epub ahead of print] Application: IF. Species: RatDixon BJ, Chen D, Zhang Y, Flores J, Malaguit J, Nowrangi D, Zhang JH, Tang J. (2016) "Intranasal Administration of Interferon Beta Attenuates Neuronal Apoptosis via the JAK1/STAT3/BCL-2 Pathway in a Rat Model of Neonatal Hypoxic-Ischemic Encephalopathy." ASN Neuro. PMID:27683877 8(5) Application: IF. Species: RatWang JY, Huang YN, Chiu CC, Tweedie D, Luo W, Pick CG, Chou SY, Luo Y, Hoffer BJ, Greig NH, Wang JY (2016) "Pomalidomide mitigates neuronal loss, neuroinflammation, and behavioral impairments induced by traumatic brain injury in rat." J Neuroinflammation. PMID:27353053 13(1):168 Application: IF. Species: RatWang G, Manaenko A, Shao A, Ou Y, Yang P, Budbazar E, Nowrangi D, Zhang JH, Tang J (2016) "Low-density lipoprotein receptor-related protein-1 facilitates heme scavenging after intracerebral hemorrhage in mice." J Cereb Blood Flow Metab. PMID:27317656 [Epub ahead of pring] Application: IF. Species: MouseSafdaria BK, Siab TC, Wattchow DA, Smid SD (2016) "Effects of pro-inflammatory cytokines, lipopolysaccharide and COX-2 mediators on human colonic neuromuscular function and epithelial permeability." Cytokine. PMID:27177092 83:231-8 Application: IF. Species: HumanLian YD, Chen ZX, Zhu KR, Sun SY, Zhu LP (2016) "Effect of equipotent doses of bupivacaine and ropivacaine in high-fat diet fed neonatal rodent model." Brazilian Journal of Anesthesiology [In Press] Application: IF. Species: RatLaunay PS, Reboussin E, Liang H, Kessal K, Godefroy D, Rostene W, Sahel JA, Baudouin C, Parsadaniantz SM, Le Goazigo AR (2016) "Ocular inflammation induces trigeminal pain, peripheral and central neuroinflammatory mechanisms." Neurobiology of Disease. PMID:26747211 88:16-28 Application: IH. Species: MouseKhan D, Dupper A, Deshpande T, Graan PN, Steinhäuser C, Bedner P. (2016) "Experimental febrile seizures impair interastrocytic gap junction coupling in juvenile mice." J Neurosci Res. PMID:26931373 [Epub ahead of print] Application: IF. Species: MouseBye N, Christie KJ, Turbic A, Basrai HS, Turnley AM (2016) "Rho kinase inhibition following traumatic brain injury in mice promotes functional improvement and acute neuron survival but has little effect on neurogenesis, glial responses or neuroinflammation." Exp Neurol. PMID:26896832 [Epub ahead of print] Application: IH. Species: MouseBedner P, Dupper A, Hüttmann K, Müller J, Herde MK, Dublin P, Deshpande T, Schramm J, Häussler U, Haas CA, Henneberger C, Theis M, Steinhäuser C (2015) "Astrocyte uncoupling as a cause of human temporal lobe epilepsy." Brain. PMID:25765328 [Epub ahead of print] Application: IH 4%PFA, vibratome 40um sections. Species: MouseRamesh G, Didier PJ, England JD, Santana-Gould L, Doyle-Meyers LA, Martin DS, Jacobs MB, Philipp MT (2015) "Inflammation in the Pathogenesis of Lyme Neuroborreliosis." Am J Pathol. PMID:25892509 [Epub ahead of print] Application: IH with Z-fixative, 5 micron sections. Species: Rhesus MonkeySuda S, Ueda M, Nito C, Nishiyama Y, Okubo S, Abe A, Aoki J, Suzuki K, Sakamoto Y, Kimura K (2015) "Valproic acid ameliorates ischemic brain injury in hyperglycemic rats with permanent middle cerebral occlusion." Brain Res. PMID:25721785 1606:1-8 Application: IH. Species: RatMayeux JP, Teng SX, Katz PS, Gilpin NW, Molina PE (2015) "Traumatic brain injury induces neuroinflammation and neuronal degeneration that is associated with escalated alcohol self-administration in rats." Behav Brain Res. PMID:25446758 279:22-30 Application: IH. Species: RatYang LY, Chu YH, Tweedie D, Yu QS, Pick CG, Hoffer BJ, Greig NH, Wang JY (2015) "Post-trauma administration of the pifithrin-α oxygen analog improves histological and functional outcomes after experimental traumatic brain injury." Exp Neurol. PMID:25819102 269:56-66 Application: IH. Species: RatRen YY, Zhang HQ, Duan SH, Wang SD (2015) "Influence of Dexmedetomidine on Toxicity of IntrathecalKetamine on Neonatal Rat Spinal Function." Tropical Journal of Pharmaceutical Research. 14(3):469-77 Application: IH. Species: RatIseli CE, Merwin WH 3rd, Klatt-Cromwell C, Hutson KA, Ewend MG, Adunka OF, Fitzpatrick DC, Buchman CA (2014) "Effect of Cochlear Nerve Electrocautery on the Adult Cochlear Nucleus." Otol Neurotol. PMID: 25280052 [Epub ahead of print] Application: IH(P)Saito T, Nito C, Ueda M, Inaba T, Kamiya F, Muraga K, Katsura K, Katayama Y (2014) "Continuous oral administration of atorvastatin ameliorates brain damage after transient focal ischemia in rats." Life Sci. PMID: 24333133 94(2):106-14 Application: IH. Species: RatHayashida K, Sano M, Kamimura N, Yokota T, Suzuki M, Ohta S, Fukuda K, Hori S (2014) "Hydrogen inhalation during normoxic resuscitation improves neurological outcome in a rat model of cardiac arrest independently of targeted temperature management." Circulation. PMID: 25366995 130(24):2173-80 Application: IH. Species: Rat |
| Related Products: |
This product is equivalent to discontinued product AG310 (FJB) from Merck-Millipore
TR-100-FTJ | TR-100-FJ | TR-160-FJC (equivalent to discontinued product AG325 (FJB) from Merck-Millipore) |
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