Fluorescent proteins, like EBFP, can be used as protein "tags" to study the subcellular localization of proteins and/or their translocation upon stimulation and/or as markers for transfections in transient and stable expression systems.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized in a 10 mM ammonium bicarbonate buffer. Each vial contains 2 mg of BSA.
Storage Temp:
Store lyophilized/reconstituted at 2-8°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Mouse
Species Reactivity:
BFP, GFP, YFP
Immunogen:
Recombinant EBFP (NCBI accession number AX_766758 REGION: 1-717, expression vector pGEX-1N), expressed in E.coli.
Recommended antibody concentration: 0.5 mg/ml (when dissolved at 0.5 mg/ml, the BSA concentration will be 1%). Recommended solvent; 100 mM PBS or Tris-HCl, pH 7.0 •Additional sodium azide ( up to 0.05%) is recommended for long term storage. •For a 0.5 mg/ml antibody concentration in 1% BSA, dissolve in 200 ul buffer.
Related products:
AS18 4175 | Anti-GFP | Green Fluorescent Protein (monoclonal), mouse antibodies AS15 2987 | Anti-GFP (affinity purified), rabbit antibodyAS15 3000 | Anti-GFP (protein A purified), rabbit antibodyAS15 2999 | Anti-GFP (total IgG), rabbit antibodyAS15 3001 | Anti-GFP, peroxidase conjugated, rabbit antibodyAS15 3002 | GFP (purified recombinant protein) Other GFP-tag antibodies.Other tag antibodies. Tag antibodies Tag2021
HA-tag is derived from a human influenza hemagglutinin HA-molecule corresponding to amino acids 96-106 and is used as a general epitope tag in expression vectors. An anti-HA antibody can then be used to detect the protein on western blot or to immunoprecipitate the recombinant protein in binding studies with transfected cells.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS pH 7.2, containing 0.1% sodium azide.
Storage Temp:
Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Host Animal:
Chicken
Species Reactivity:
HA-tagged proteins
Immunogen:
KLH-conjugated synthetic petide YPYDVPDYA of influenza virus hemagglutinin.
Applications:
ELISA (ELISA), Flow cytometry (Flowcyt), Western blot (WB)
His-Tag is a polyhistidine tag which consists of 6 histidine residues introduced on N- or C-terminus of the protein. The polyhistidine-tag can be used for recombinant protein detection using specific antibodies and it is not conjugated to any dye or enzyme.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid in PBS with 0.08% sodium azide.
Storage Temp:
Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
HA-tag is derived from a human influenza hemagglutinin HA-molecule corresponding to amino acids 96-106 and is used as a general epitope tag in expression vectors. An anti-HA antibody can then be used to detect the protein on western blot or to immunoprecipitate the recombinant protein in binding studies with transfected cells.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid in PBS with 0.08% sodium azide
Storage Temp:
Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Host Animal:
Mouse
Species Reactivity:
HA-tagged proteins
Immunogen:
9 amono acid long synthetic petide to influenza virus hemagglutinin.
Chicken anti-DYKDDDDK is a primary antibody which binds to DYKDDDDK (Sigma FLAG®).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20°C; avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Antibody was analyzed using amino-terminal Met-Flag-BAP, amino- terminal FLAG-BAP and carboxy-terminal FLAG-BAP fusion proteins and Invitrogen Positope R900-40.
His-Tag is a polyhistidine tag which consists of 6 histidine residues introduced on N- or C-terminus of the protein. The polyhistidine-tag can be used for recombinant protein detection using specific antibodies and it is not conjugated to any dye or enzyme.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
GST-tag (glutathione S-transferase) is a tag added to a protein of interest as a fusion protein to unable purification and detection. The tag is 220 amino acid long, ca. 26 kDa which makes it quite large compare to Myc or FLAG tags.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Expected Species:
native and denatured forms of purified GST or GST fusion proteins
Overnight incubation of this antibody with dilution 1:5 000 with 0,5 µg of GST-tagged protein produced strong background but works fine with samples of total Escherichia coli lysate if GST-tagged protein is expressed in low amounts.
Application Details:
1: 1: 1000 (ELISA), 1: 2000 (WB)
Purity:
Affinity purified using solid phase GST
Reconstitution:
For reconstitution add 50 µl of sterile water.
Related products:
collection of antibodies to various tagsPlant and algal protein extraction buffer Secondary antibodies Tag antibodies Tag2021
Molecular Weight:
26 kDa
Not reactive in:
no confirmed exceptions from predicted reactivity are currently known
Special application note:
Exact working dilution for each application needs to be determined experimentally.
LUC (Luciferase) from Photinus pyralis (Common eastern firefly) (Lampyris pyralis), light producing beetle is an enzyme in the light production.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
LUC
Immunogen:
KLH-conjugated synthetic peptide derived from N-terminal part of LUC protein sequence of Photinus pyralis, UniProt: Q27758
GUS (Beta-glucuronidase) is an enzyme which when incubated with colorless or non-fluorescent substrates, can catalyze reaction of their transformation into coloured or fluorescent products.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
GUS
Immunogen:
KLH-conjugated synthetic peptide derived from Escherichia coli GUS protein at amino acid 358.
Antibodies to other reporter proteinsSecondary antibodies Tag antibodies Tag2021
Molecular Weight:
depends upon a MW of a protein which is expressed with GUS
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Nizkorodova et al. (2020). The Effect of Translation Promoting Site (TPS) on Protein Expression in E. coli Cells. Mol Biotechnol (2020). doi.org/10.1007/s12033-020-00251-1Nikorodova and Isakov (2019). New insights into the mechanism of action of e-element enhancer in E.coli. Eurasian Journal of Applied Biotechnology 2/2019
Special application note:
This antibody will not work with pCAMBIA vectors due to mutation starting at amino acid position 358.
GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°C, or in small aliquots at -20°C. Avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Native GFP, Recombinant GFP (E.coli), all variants of GFP, including EGFP.
Immunogen:
Highly purified native GFP protein derived from Aequorea victoria, UniProt: P42212
Applications:
ELISA (ELISA), Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)
Wieczorek et al. (2020) Development of a New Tomato Torrado Virus-Based Vector Tagged with GFP for Monitoring Virus Movement in Plants. Viruses. 2020 Oct 20;12(10):1195. doi: 10.3390/v12101195. PMID: 33092281; PMCID: PMC7588970.Kulich et al. (2018). Deubiquitinase OTU5 affects Root Responses to Phosphate Starvation. Plant Physiol. 2018 Jan 4. pii: pp.01693.2017. doi: 10.1104/pp.17.01693.
Special application note:
10 mM TRIS buffer pH 8.0 containing 0.02% sodium azide
FLAG-tag is a tag that can be added to a protein sequence motif DYKXXD. It can be used for affinity chromatography, to separate recombinant protein from wt proteins. It can also be used to isolate protein complexes with multiple subunits.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid in 10mM PBS, pH 7.2, with 1% BSA and 0.05% sodium azide
Storage Temp:
Store at -20°C for up to 2 years.
Host Animal:
Mouse
Species Reactivity:
N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins
Immunogen:
KLH-conjugated DYKDDDDK (FLAG) synthetic peptide
Applications:
Dot blot (Dot), ELISA (ELISA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB)
1 : 1000-5000 (1-5ng) (WB) (incubate for one hour at room temperature). 1 : 500-1 :2000 (IS) For best results with other assays (Dot, ELISA, IP), please determine optimal working dilution by end user
Purity:
Protein A purified
Related products:
AS15 2952 | Anti-DYKDDDDK (binds to Sigma FLAG®), ALP conjugated, mouse monoclonal antibodiesAS15 2950 | Anti-DYKDDDDK (binds to Sigma FLAG®), DyLight® 488 conjugated, mouse monoclonal antibodiesAS15 2951 | anti-DYKDDDDK (binds to Sigma FLAG®), DyLight® 550 conjugated, mouse monoclonal antibodiesAS15 2947 | anti-DYKDDDDK (binds to Sigma FLAG®), DyLight® 650 conjugated, mouse monoclonal antibodiesAS15 2948 | anti-DYKDDDDK (binds to Sigma FLAG®), Europium conjugated, mouse monoclonal antibodiesAS15 2949 | anti-DYKDDDDK (binds to Sigma FLAG®), SureLight® R-Phycoerythrin conjugated,mouse monoclonal antibodiesAS15 3036 | anti-DYKDDDDK (binds to Sigma FLAG®),mouse monoclonal antibodiesAS15 3037 | anti-DYKDDDDK (binds to Sigma FLAG®), rabbit antibodiesother tag antibodiesSecondary antibodies Tag antibodies Tag2021
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Special application note:
Working dilution for Dot Blot, ELISA and IP needs to be determined experimentally.Antibody is purified on Protein A.
GST-tag (glutathione S-transferase) is a tag added to a protein of interest as a fusion protein to unable purification and detection. The tag is 220 amino acid long, ca. 26 kDa which makes it quite large compare to Myc or FLAG tags.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Mouse
Species Reactivity:
Native and denatured forms of purified GST or GST fusion proteins.
Expected Species:
Native and denatured forms of purified GST or GST fusion proteins
Immunogen:
GST protein
Applications:
Western blot (WB), Dot blot (Dot), ELISA (ELISA), Immunoprecipitation (IP), Immunohistochemistry (IHC)
c-Myc is derived from Myc proto-oncogene protein. A short peptide located between amino acids 408-420 is used as an epitope tag, that is easily recognized by tag specific antibodies. This is a universal detection reagent which will allow detection of any tag containing protein.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized in PBS pH 8.5 with 0.004 % sodium azide
Storage Temp:
Store lyophilized antibodies at -20°C and reconstituted antibodies at 4°C. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Chicken
Species Reactivity:
c-Myc
Expected Species:
c-Myc
Immunogen:
KLH-conjugated peptide, sequence MEQKLISEEDLNE, human c-Myc UniProt: Q6LBK7
aadA - aminoglycoside 3"adenyltransferase is an enzyme with nucleotidyltransferase activity. Plastid transformation in tobacco involves expression of aadA cassette, which confers resistance to spectinomycin and streptomycin and allows for transformat selection.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
aadA casesette in Nicotiana tabacum, Chlamydomonas reinhardtii
Expected Species:
Acinetobacter baumannii, Enterobacter agglomerans, Klebsiella pneumoniae, Klebsiella oxytoca, Kluyvera ascorbata, Pseudomonas aeruginosa, Salmonella enterica subsp. enterica serovar Worthington, Staphylococcus aureus, Serratia sp., Shigella flexneri Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from known aadA1 protein sequences
antibody collection for Arabidopsis thalianaPlant protein extraction bufferSecondary antibodies Tag2021
Molecular Weight:
30 | 30 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Lauersen et a. (2018). Phototrophic production of heterologous diterpenoids and a hydroxy-functionalized derivative from Chlamydomonas reinhardtii. Metab Eng. 2018 Jul 12;49:116-127. doi: 10.1016/j.ymben.2018.07.005.
Special application note:
This product can be sold containing ProClin if requested.
Research Area:
GAL4 / GUS / LUC / others
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