DNA methyltransferase 3 beta (DNMT3B), is a DNA methyltransferase believed to function in de novo methylation, rather than in maintenance methylation. DNMT3B localises to the nucleus and its expression is developmentally regulated. It has been shown to interact with CBX5, DNMT1, DNMT3A, NCAPG, SMC2, KIF4A, SUMO1 and UBE2I.
DNMT3B is has N-terminal regulatory and C-terminal catalytic domains linked by repeated GK dipeptides (Glycine-Lysine-repeats). The N-terminal domain is responsible for nucleus localisation and plays a regulatory role. It also contains a proliferating cell nuclear antigen-binding domain, a cysteine rich zinc finger DNA binding motif (ATRX), a polybromo homology domain (PHD) and a PWWP tetrapeptide chromatin-binding domain.
Product Type:
NS Reagents Antibody
Antibody Type:
Monoclonal
Format:
100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide.
DNA methyltransferase 3 beta (DNMT3B), is a DNA methyltransferase believed to function in de novo methylation, rather than in maintenance methylation. DNMT3B localises to the nucleus and its expression is developmentally regulated. It has been shown to interact with CBX5, DNMT1, DNMT3A, NCAPG, SMC2, KIF4A, SUMO1 and UBE2I.
DNMT3B is has N-terminal regulatory and C-terminal catalytic domains linked by repeated GK dipeptides (Glycine-Lysine-repeats). The N-terminal domain is responsible for nucleus localisation and plays a regulatory role. It also contains a proliferating cell nuclear antigen-binding domain, a cysteine rich zinc finger DNA binding motif (ATRX), a polybromo homology domain (PHD) and a PWWP tetrapeptide chromatin-binding domain.
Product Type:
NS Reagents Antibody
Antibody Type:
Monoclonal
Format:
100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide.
DNA methyltransferase 1 (DNMT1) associates with the replication machinery and restores symmetrical methylation at hemimethylated CpG sites generated by the semi-conservative DNA replication process. It maintains methylation patterns with high fidelity and is essential for embryonic development and genome integrity.
DNMT1 is one of the most abundant DNA methyltransferase in mammalian tissues. It comprises a regulatory N-terminal region and a C-terminal catalytic domain connected by a linker of seven glycine-lysine repeats. The C-terminal domains contain all ten catalytic motifs identified in bacterial DNA (cytosine-5) methyltransferases. The N-terminal part contains a PCNA binding domain (PBD), a heterochromatin targeting sequence (TS), a CXXC-type zinc finger domain and two Bromo-Adjacent Homology domains (BAH1 and BAH2).
DNMT1 interacts with RNA polymerase II, some RNA-binding proteins, and some specific Dnmt1-inhibitory RNA molecules involved in chromatin organisation, DNA repair, cell cycle regulation, and apoptosis.
Product Type:
NS Reagents Antibody
Antibody Type:
Monoclonal
Format:
100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide.
DNA methyltransferase 1 (DNMT1) associates with the replication machinery and restores symmetrical methylation at hemimethylated CpG sites generated by the semi-conservative DNA replication process. It maintains methylation patterns with high fidelity and is essential for embryonic development and genome integrity.
DNMT1 is one of the most abundant DNA methyltransferase in mammalian tissues. It comprises a regulatory N-terminal region and a C-terminal catalytic domain connected by a linker of seven glycine-lysine repeats. The C-terminal domains contain all ten catalytic motifs identified in bacterial DNA (cytosine-5) methyltransferases. The N-terminal part contains a PCNA binding domain (PBD), a heterochromatin targeting sequence (TS), a CXXC-type zinc finger domain and two Bromo-Adjacent Homology domains (BAH1 and BAH2).
DNMT1 interacts with RNA polymerase II, some RNA-binding proteins, and some specific Dnmt1-inhibitory RNA molecules involved in chromatin organisation, DNA repair, cell cycle regulation, and apoptosis.
Product Type:
NS Reagents Antibody
Antibody Type:
Monoclonal
Format:
100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide.
DNA methyltransferase 3A (DNMT3A) belongs to the mammalian methyltransferase gene family which is responsible for tissue-specific gene expression. DNMT3A together with other methyltransferases carries out de novo methylation of cytosine residues in CpG islands by the enzymatic addition of methyl residues from S-adenosyl-L-methionine to the 5-carbon atom of the cytosine ring.
DNMT3A also represses key genes enabling the coupling of insulin secretion to glucose levels during beta cell maturation. Loss of Dnmt3a in pancreatic beta cells prevents this developmental metabolic reprogramming, resulting in loss of GSIS (Glucose-Stimulated Insulin Secretion)). Dnmt3a overexpression has been shown in several cancers including AML (Acute Myeloid Leukemia).
Product Type:
NS Reagents Antibody
Antibody Type:
Monoclonal
Format:
100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide.
DNA methyltransferase 3A (DNMT3A) belongs to the mammalian methyltransferase gene family which is responsible for tissue-specific gene expression. DNMT3A together with other methyltransferases carries out de novo methylation of cytosine residues in CpG islands by the enzymatic addition of methyl residues from S-adenosyl-L-methionine to the 5-carbon atom of the cytosine ring.
DNMT3A also represses key genes enabling the coupling of insulin secretion to glucose levels during beta cell maturation. Loss of Dnmt3a in pancreatic beta cells prevents this developmental metabolic reprogramming, resulting in loss of GSIS (Glucose-Stimulated Insulin Secretion)). Dnmt3a overexpression has been shown in several cancers including AML (Acute Myeloid Leukemia).
Product Type:
NS Reagents Antibody
Antibody Type:
Monoclonal
Format:
100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide.
DNA methyltransferase 3A (DNMT3A) belongs to the mammalian methyltransferase gene family which is responsible for tissue-specific gene expression. DNMT3A together with other methyltransferases carries out de novo methylation of cytosine residues in CpG islands by the enzymatic addition of methyl residues from S-adenosyl-L-methionine to the 5-carbon atom of the cytosine ring.
DNMT3A also represses key genes enabling the coupling of insulin secretion to glucose levels during beta cell maturation. Loss of Dnmt3a in pancreatic beta cells prevents this developmental metabolic reprogramming, resulting in loss of GSIS (Glucose-Stimulated Insulin Secretion)). Dnmt3a overexpression has been shown in several cancers including AML (Acute Myeloid Leukemia).
Product Type:
NS Reagents Antibody
Antibody Type:
Monoclonal
Format:
100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide.
DNA methyltransferase 3A (DNMT3A) belongs to the mammalian methyltransferase gene family which is responsible for tissue-specific gene expression. DNMT3A together with other methyltransferases carries out de novo methylation of cytosine residues in CpG islands by the enzymatic addition of methyl residues from S-adenosyl-L-methionine to the 5-carbon atom of the cytosine ring.
DNMT3A also represses key genes enabling the coupling of insulin secretion to glucose levels during beta cell maturation. Loss of Dnmt3a in pancreatic beta cells prevents this developmental metabolic reprogramming, resulting in loss of GSIS (Glucose-Stimulated Insulin Secretion)). Dnmt3a overexpression has been shown in several cancers including AML (Acute Myeloid Leukemia).
Product Type:
NS Reagents Antibody
Antibody Type:
Monoclonal
Format:
100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide.
DNA methyltransferase 2 (DNMT2) is highly conserved cytosine-C5 methyltransferase that contains all 10 sequence motifs that are conserved among m5C MTases, including the consensus S-adenosyl-L-methionine-binding motifs and the active site ProCys dipeptide. It is a relatively small protein of 391 amino acids and lacks the large N-terminal domains present in the DNMT1 and DNMT3. DNMT2 is ubiquitously expressed with multiple mRNA species in most human and mouse adult tissues. It is variably expressed in human cancer cell lines.
Product Type:
NS Reagents Antibody
Antibody Type:
Monoclonal
Format:
100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide.
DNA methyltransferase 2 (DNMT2) is highly conserved cytosine-C5 methyltransferase that contains all 10 sequence motifs that are conserved among m5C MTases, including the consensus S-adenosyl-L-methionine-binding motifs and the active site ProCys dipeptide. It is a relatively small protein of 391 amino acids and lacks the large N-terminal domains present in the DNMT1 and DNMT3. DNMT2 is ubiquitously expressed with multiple mRNA species in most human and mouse adult tissues. It is variably expressed in human cancer cell lines.
Product Type:
NS Reagents Antibody
Antibody Type:
Monoclonal
Format:
100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide.
DNA methyltransferase 3-like (DNMT3L) as a catalytically inactive regulatory factor of DNA methyltransferases that can either promote or inhibit DNA methylation. It is not thought to function as a DNA methyltransferase itself as it does not contain the amino acid residues necessary for methyltransferase activity. However, it is essential for the function of DNMT3A and DNMT3B and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1 and plays a key role in embryonic stem cells and germ cells.
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal
Format:
100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide.
DNA methyltransferase 3-like (DNMT3L) as a catalytically inactive regulatory factor of DNA methyltransferases that can either promote or inhibit DNA methylation. It is not thought to function as a DNA methyltransferase itself as it does not contain the amino acid residues necessary for methyltransferase activity. However, it is essential for the function of DNMT3A and DNMT3B and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1 and plays a key role in embryonic stem cells and germ cells.
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal
Format:
100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide.
DNA (cytosine-5-)-methyltransferase 3-like (DNMT3L), DNA methyltransferase 3-like.
Antibody Isotype:
IgG
Application Details:
WB: 2-4ug/ml
Antibody Number:
10039
Category:
Primary Antibodies
Research Areas:
Transcription and Translation, Nucleic Acid Modification, Protein Modification, Transcription, Chromatin
NS Product Area:
Protein and Nucleic Acid Modification
Purification:
Protein A Purified
Uniprot ID:
Q9UJW3
Gene ID:
29947
Release Date:
December 2017
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