Product Search Results : combo-seq

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Product - please click for more details Brand Cat No Size Price (£)

Bioo Scientific - NGS NOVA-5139-01 8 rxns £594.00
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Product Detail: The NEXTFLEX® Combo-Seq™ Kit enables the user to generate combined mRNA and small RNA libraries in a single workflow using 1 ng – 100 ng of total RNA inputs, without requiring upfront costly and tedious rRNA depletion or poly(A) selection. The kit utilizes patented and patent-pending technology that allows processing of mRNA fragments and small RNA for library construction. Like the best-in-class, NEXTFLEX® small RNA-seq kit v3, the NEXTFLEX® Combo-Seq™ mRNA/miRNA library preparation kit utilizes adapters with randomized ends to greatly reduce bias compared to standard protocols. This allows a more accurate representation of small RNA in the starting material and cost-saving due to efficient sequencing. Adapter-dimer reducing techniques built into the protocol enables gel-free workflow from inputs as low as 1 ng of total RNA. As a result, the kit is streamlined and automation friendly from start-to-finish. Libraries with robust yield can be prepared within one day using a manual or automated protocol.
Background Info: All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type: NGS - mRNA/miRNA - Illumina Compatible
Applications: Rapid Directional mRNA
Data Sheet: PLEASE CLICK HERE TO DOWNLOAD
Search Terms: Rapid Directional mRNA Small RNA
Additional Info: This kit can be used to generate 48 RNA libraries and contains the reagents necessary for Poly(A) enrichment, library preparation, and 24 unique barcoded adapters for multiplexing. There are six steps involved in preparing RNAs for sequencing: fragmentation, first strand and second strand synthesis, adenylation, adapter ligation, and PCR amplification. Strandedness is retained by adding dUTP during the second strand synthesis step and subsequent cleavage of the uridine-containing strand using Uracil DNA Glycosylase. The strand that’s sequenced is the cDNA strand. This kit contains the necessary reagents to process the user’s purified RNA sample through preparation and amplification for loading onto flow cells for sequencing.

[MULTIPLEXING]

Eight UDI barcoded primers are included in the small reaction size NEXTFLEX® Combo-Seq™ kit and forty-eight UDI barcoded primers are included in the large reaction size kit.

KEY FEATURES
• Combined library prep workflow for both small RNA and mRNA sequencing
• Completely gel-free protocol from inputs as low as 1ng
• Compatible with total RNA inputs, requiring no poly(A) selection or rRNA depletion
• Complete kit solution, including cleanup/size selection beads and barcodes
• Automation-ready on the PerkinElmer® Sciclone® G3 NGS/NGSx workstation
Application Details: Please email tech@nktscientific to request a copy of the datasheet