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Newmarket Scientific

Product Search Results: hDFBs

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Product Search Results: hDFBs

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Vitality V-hDFB 1 Million Cells £506.00
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Product Detail: Human dermal fibroblasts (HDFs) are a potential source of somatic cells for genetic manipulation and tissue engineering. Confirmation of cytogenetic stability of these cells is an essential step for cell nuclear transfer and generation of a suitable and functional induced pluripotent stem cells line.

Dermal fibroblasts are the main cell type present in skin connective tissue (dermis). Fibroblasts interact with epidermal cells during hair development and in interfollicular skin. Moreover, they play an essential role during cutaneous wound healing and in bioengineering of skin. Hence, culture of primary fibroblast is gaining in importance. In addition, fibroblasts established from skin biopsies provide a powerful tool for investigating normal skin physiology or specific disease states.

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Background Info: Product Quality Control:

Rigid quality control testing is performed for each batch (both cell donors and cell cultures). Cultured cells are tested for cell identity, purity, potency, viability and suitability for the intended use.

Before cryopreservation cultured MSCs (P2-P3) are characterised by flow cytometry analysis for identity by a comprehensive panel of markers [CD73/CD90/CD105 (positive) and CD14/CD34/CD45/HLA-DR (negative)] as proposed by the ISCTMSC committee position statement, 2006.

A multilineage differentiation assay into adipogenic and osteogenic directions is performed for each MSCs batch expanded in vitro. In addition, all donors and cell cultures have been screened for the absence of the following infectious agents: HIV-1/2, HBV, HCV, HSV1, HSV2, CMV, EBV, HHV6, Treponema pallidum, Toxoplasmagondii, Chlamydia trachomatis, Ureaplasma urealyticum, Ureaplasma parvum and microbial contaminants (bacteria & fungi,Mycoplasma hominis and Mycoplasma genitalium).

MSC cultures’ genetic stability is verified by karyotype evaluation (GTG banding).
Product Type: Stem Cells and Cell Lines
Format: 1 Million Cells/Vial
Storage Temp: -80 C
Applications: For Research Use Only
Alternative Name: hDFBSC
Additional Info: All our Stem Cell and Cell Line products are collected with full informed consent.

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