Newmarket Scientific
Newmarket Scientific

Product Search Results: v5-v6

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Product Search Results: v5-v6

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Bioo Scientific - NGS NOVA-4205-02 24 rxns £409.00
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Product Detail: The NEXTflex™ 16S V5 – V6 Amplicon-Seq Library Prep Kit is designed for bacterial profiling of plant samples using degenerate primers that target the fifth and sixth variable regions of the 16S rRNA gene. Bacterial profiling in plant samples can be difficult because of the homology between bacterial 16S rRNA and chloroplast 16S rRNA. The use of primers binding to 16S hypervariable regions V 1 – V4, which are traditionally used for bacterial profiling, amplify both the bacterial and plant 16S rRNA genes, skewing results. NEXTflex™ 16S V5 – V6 Amplicon-Seq libraries are compatible with paired-end sequencing on the Illumina® MiSeq® sequencing platform and are ideal for monitoring population fluctuations of microbial communities.
Background Info: All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type: NGS - 16s V5-V6
Applications: 16s V5-V6, Metagenomics
Search Terms: 16s V5 - V6, Metagenomics
Additional Info: The NEXTflex 16S V5 – V6 Amplicon-Seq Kit contains enough material to prepare 8, 24, 96, or 192 amplicon-seq libraries from bacterial genomic DNA for sequencing using the Illumina® MiSeq® platform. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C. This kit is shipped on dry ice.

[Optimized Protocol Offers Lower PCR Bias and Fewer Off-target Reads]
The NEXTflex 16S V5 – V6 Amplicon-Seq Kit incorporates a second PCR step in the protocol for the addition of the sample-specific index, which reduces off-target reads encountered during amplicon sequencing.

[384 Barcodes Available for Cost-effective 16S V5 – V6 rRNA Sequencing]
The NEXTflex 16S V5 – V6 Amplicon-Seq Library Prep Kit can be used for multiplexing up to 384 samples, to greatly reduce the cost of sequencing. The barcodes are offered in sets of 4, 12, 48, or 96 barcodes and supplied with 2 reactions worth of each barcode.

[Low Input Requirements for 16S rRNA Sequencing]
As little as 1 ng of genomic DNA can be used to generate libraries using the NEXTflex 16S V5 – V6 Amplicon-Seq Library Prep Kit.

• Fast and easy library prep protocol for 16S profiling of bacteria in plant samples
• Low input - Only 1 ng of input required
• Multiplex up to 384 libraries
• Custom sequencing primers are not required
• Automation protocols are now available for the PerkinElmer® Sciclone® NGS and NGSx Workstation to automate your 16S sequencing
• Functionally validated with Illumina® sequencing platforms

For larger volume requirements, customized and bulk packaging is available. Please email us to for further information.
Catalogue Number Update: Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 4205-02