Apop Biosciences
CalRexin™ Imaging reagents for Apoptosis detection
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Apop Biosciences provides CalRexin™, a suite of proprietary imaging reagents that are designed for imaging stressed, dying and apoptotic cells. CalRexin™ target the calcitonin receptor, a novel marker of Programmed Cell Death (PCD). It is a powerful addition to the PCD toolkit, that can easily be integrated into existing workflows.
CalRexin™ (RUO) can be utilised for live cell assays, on their own or multiplexed alongside other imaging reagents or antibodies to study pre-apoptotic stress and events leading to apoptosis. Unlike alternatives, they are stable without the spectre of false positives.
CalRexin™ is available conjugated to a range of fluorophores including:
Advantages: • Simple to use • High sensitivity, detect sub-populations in FACS of dying cells • Enhanced stability (> 1 yr) • Multiple applications (FACS, Operetta, confocal microscopy) • Calcium independence for assays • No false positives vs Annexin
Confocal microscopy image: CalRexin™:568 accumulating in dying cells.
• MG63 cancer cells treated with a cytotoxin. • Green cells express tubulin and are intact living cells that exclude CalRexin™:568. • Red cells are dying, tubulin is degraded and CalRexin™:568 accumulates.
Image taken from: Furness, S., Hare, D., Kourakis, A. et al. A novel ligand of calcitonin receptor reveals a potential new sensor that modulates programmed cell death. Cell Death Discovery 2, 16062 (2016). https://doi.org/10.1038/cddiscovery.2016.62
For more information, please email tech@nktscientific.com or contact us here: Newmarket Scientific - Request a quotation |
