Newmarket Scientific
Newmarket Scientific








Antibodies


Primary and secondary antibodies


Affinity Biosciences logo   Agrisera logo     Biosensis logo    Hansabiomed logo  ImmunoReagents logo  ImmunoStar logo  Monosan logo  NS Reagents logo         Optibodies logo          

 


 

Our antibody ranges include:

NS Reagents antibodies (for Neuroscience and Protein/DNA modification and cell signalling)

Antibodies for exosome research

Neuroscience antibodies

NS Reagents - Featured antibodies (Anti-TDP43 antibodies)

Optibodies from Nordic Biosite

Phospho-specific antibodies from Affinity Biosciences

Antibodies for Plant Research

Highlighted Monosan Antibodies for Cancer Research

Antibodies for Breast Cancer Drug Resistance

Secondary Antibodies

 

And we are so confident in the quality of our antibodies that they all come with a 100% guarantee.

 

We also have our NSR prestained protein ladder for Western blotting work.

 

Please follow the links above to see the antibodies available in each category or (because we do have a lot of antibodies) use the search box above to find a specific antibody or email us (tech@nktscientific.com) and we'll find it for you.

 



Anti-HIS tag antibodies - 30 % off

 

Tag antibodies from Agrisera

 

Agrisera His-Tag antibodies are currently 30% off the listed price until the end of March 2020

 

AS19 4255 | His-tag | 6xHis (polyclonal), unconjugated

 

AS15 2935| His-tag, ALP conjugated (monoclonal)

 

AS15 2930 | His-tag, HRP conjugated (monoclonal)

 

To use this offer simply add “HIS30” to your order.

 

Valid until end of March 2020

 

If you have any questions about this offer or would like further information about any of the Agrisera products, please contact us at tech@nktscientific.com

 



TDP43 Antibodies

TAR DNA-binding protein 43 (TDP43) binds both DNA and RNA and has multiple functions in transcriptional repression, pre-mRNA splicing and translational regulation. Characterisation of transcriptome-wide binding sites shows that thousands of RNAs are bound by TDP43 in neurons.

 

Hyperphosphorylated, fragmented and ubiquitinated forms of TDP-43 have been identified as core components of cytosolic inclusions in sporadic ALS (amyotrophic lateral sclerosis) and FTLD (frontotemporal lobar degeneration). As well as ALS and FTLD, mutations in TDP-43 have also been associated Parkinson's disease and Alzheimer's disease. 

 

TDP43 is a key target protein in research around numerous neurodegenerative conditions. Read More...

 

NS Reagents TDP43 Antibody (Cat-AA17-100105) Image 1 NS Reagents TDP43 Antibody (Cat-AA17-100105) Image 2

 

ALS patient line showing proteinopathy.

 

 

TDP43: Cat No: AX17-10010

Lot AX17-1808-0002

Technique: ICC

Secondary Ab: Alexa 488

Primary Ab dilution: 1:200

 


 

Affinity TDP43 Phospho (Cat-AF7365) Image 3 Affinity TDP43 Phospho (Cat-AF7365) Image 2

 

ALS patient line showing

nuclear TDP43 phosphorylation.

 

Affinity - phospho TDP43 Ab Cat No: AF7365

Lot 19p1575

Technique: ICC

Secondary Ab: Alexa 488

Primary Ab dilution: 1:100

 


Images courtesy of Dr Laura Ferraiuolo and Mr Marco Destro at the University of Sheffield

 



Affinity Biosciences - The phospho-antibody specialist

 

Affinity Biosciences is a provider of innovative tools for signal transduction research with a focus on well validated phospho-specific antibodies.

 

Use our search to find your antibody (e.g. Ser724, IRE1 or Phospho-IRE1 etc).

 

Well validated phospho-specific antibodies

Validation is important for any antibody, but for Phospho-specific antibodies it is even more important as specificity is truly key to their usefulness.

 

Affinity Biosciences antibody validation begins with Mass Spec (MS) analysis of the blocking peptide to ensure the peptide has been produced correctly, followed by checking that the peptide blocks the antibody well in Western blotting with research samples showing that the antibody corresponds correctly to the blocking peptide.

 

DATASHEET IMAGES: Images of Western blots are provided on the datasheets showing blocking and control lanes (without the peptide) to show binding of the target along with images of other applications such as Immunohistochemistry, immunofluorescence etc as appropriate.

 

As an example, details of the validation of our Phospho-IRE1 (Ser724) - AF7150 antibody have been provided below:

 

Western Blot, IHC, Immunofluorescence and Peptide-ELISA for Anti Phospho-IRE1 (Ser724).

 

pIRE1 WB  pIRE1 IHC

pIRE1 Immunofluorescence  pIRE1 ELISA

Phospho-IRE1 (Ser724) - AF7150 Datasheet

 

Affinity also produce more than 500 inhibitors and SiRNAs.

 

Additionally all affinity products are covered by our 100% antibody guarantee, so you can try them without worrying whether they will work. We know they work well, but we understand you may be sceptical having tried other antibodies in the past, so now you can try them without worrying.

If they don't work, we'll give you your money back.

 

 

Other Highlighted Antibodies:

 

Anti-Phospho-ERK1/2 Antibody (Thr202/Tyr204) - AF1015

29 Citations

 

Anti-Phospho-Akt Antibody (Ser473) - AF0016

28 Citations

 

Phospho-mTOR Antibody (Ser2448) - AF3308

14 Citations 

 

Phospho-p70 S6 Kinase Antibody (Thr389/412) - AF3228

9 Citations

 

Use our search at the top of the page to find your antibody

(e.g. Ser724, IRE1 or Phospho-IRE1 etc).

 

Questions? Email us at tech@nktscientific.com.

 



Plant science antibodies

Plant Research

 

Our plant research range covers the areas below:

 

Please use the search box to find your antibody or for help use our contact form or email tech@nktscientific.com.

 

Global antibodies Bioenergetics
Carbohydrates Compartment markers
Developmental biology DNA/RNA/cell cycle
Environmental stress Epigenetics
Fermentation Food proteins
Hormones Membrane transport system
Mitochondria | Respiration Nitrogen metabolism
Photosynthesis Plant pathogens
Tag antibodies Toxins

 



Optibodies from Nordic Biosite

 

You have most likely been using antibodies in Immunohistochemistry for some time and maybe you are getting good results, but Optibodies are on another level.

 

In producing the Optibodies range we ensure that every antibody provides beautifully optimised staining. This is the key aim of the Optibodies range and any antibody that does not perform to this standard simply does not make it in to the range.

 

Optibody™ optimisation involves a great number of tests in order to find the optimal staining conditions.

 

Antigen Retrieval

Each antigen has a preferred method of antigen retrieval such as heat induced epitope retrieval using citrate buffer, as well as an enzymatic retrieval process, but the majority of antigens need alkaline antigen retrieval conditions for truly optimal staining. For this reason, Optibodies are all selected according to the performance in alkaline antigen retrieval.

 

Concentration

Antibodies generally have an optimal concentration for use, depending on the affinity of the antibody for its target and expression level of the antigen. Antibodies optimised with tissues expressing high levels of antigen may prove inadequate when staining tissues with low antigen expression. So Optibodies are optimised on a variety of tissue types and antigen expression levels to verify the performance of the Optibody in a range of situations.

 

Internal and External QC

To ensure the high quality of every Optibody, as well as our own high level of internal quality control, we also take part in external quality assurance programs to ensure our antibodies work optimally in the hands of others too.

 

Other key criteria

Another key target for each Optibody is specificity and high affinity towards the target. They are also tailored to be flexible and reliable in use, with high consistency between lots and excellent stability.

 

More information on the full Optibodies range is available on this link.

 



Exosomes

 

Extracellular Vesicles (EVs), including exosomes, express antigens with 3D conformations and/or post-translational modifications that often differ from the cellular counterpart. For this reason, most of the antibodies currently available on the market fail to recognise exosome-associated antigens with sufficient sensitivity and specificity.

 

In collaboration with Exosomics Siena Spa, HansaBioMed has validated a set of polyclonal and monoclonal antibodies against common exosomal markers (CD63, CD81, ALIX) and disease-specific exosomal markers (cancer and neurodegenerative diseases) as listed below. Follow the links for more details and pricing.

 

Anti-CD9 Anti-CD63
Anti-CD41 Anti-CD44
Anti-CD81 Anti-Caveolin1
Anti-ALIX Anti-TM9SF4
Anti-flotillin Anti-Rab5
Anti-HSP70 Anti-TSTA3

 

 

 

 

 

 

 

 

 

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Neuroscience%20antibodies

Neuroscience

 

The ImmunoStar range of antibodies for neuroscience research are put through extensive testing before release to ensure both high quality and high titer. This provides excellent reliability and lot-to-lot consistency. The antibodies have also been specifically tested for use in immunohistochemistry.

 

This range of primary antibodies was originally provided by DiaSorin (formerly INCSTAR), but in 2001 the range became Immunostar. As these antibodies have been available for many years, there are multiple references citing their use.

 

 

Details of the full range of 60+ well referenced neuroscience antibodies are available on this link.

 



Secondary antibodies

Secondary Antibodies

 

Our range of secondary antibodies includes antibodies to many primary antibody species:

 

Anti-Human, Anti-Mouse, Anti-Rabbit, Anti-Goat, Anti-Rat, 
Anti-Sheep, Anti-Guinea Pig, Anti-Hamster, Anti-Bovine, 
Anti-Donkey, Anti-Cat, Anti-Dog, Anti-Llama and Anti-Chicken

 

All secondary antibodies are available as a range of conjugates including Dylight Dyes, HRP, AP, FITC, TRITC and more. 

 

We can provide quantities from milligrams (mg) right up to grams (g) for bulk antibody requirements.

 

Follow the link above to select your secondary antibody from a list of options or if you are having difficulty locating it, simply use our contact form or email us at tech@nktscientific.com.

 



Highlighted Monosan Antibodies

 

Mouse anti Human Pan Cytokeratin, clone BS5 This antibody provides exceptional staining in carcinoma cells and is very popular in pathology labs across Europe. Try this antibody for free, to find out why. Simply use our contact form to ask for a 0.1ml sample.

 

Mouse anti-human-Pan-Cytokeratin 

Ductal breast adenocarcinoma section stained using Monosan anti human pan cytokeratin (Clone: BS5) with 1:200 dilution.

 

Mouse anti Human Granzyme B, clone GrB-7 This antibody reacts with the 33 kD human serine protease granzyme B. It does not cross react with human granzyme A. It can be used as a marker for NK-cells and activated cytotoxic T-cells (CTL).

 

This monoclonal antibody stains granzyme B expressing lymphocytes in sections of sublimate formaldehyde as well as formalin fixed paraffin embedded tissues. It does not react with other cell types. The antibody can not be used on frozen sections. The antibody is also useful for staining granzyme B protein on Western blots.

 

Granzyme B staining 

 

Mouse anti Human Desmin, clone D33 This antibody is highly reactive with desmin and does not cross react with GFAP, Keratin, Vimentin or Neurofilament. This antibody stains muscle cells in human tissues. Frozen sections show excellent staining in immunohistochemistry or immunofluorescence. On sections of formalin fixed and paraffin embedded tissue the staining is reduced. This antibody is also useful in Western blots.

 

Desmin staining

 

Mouse anti CD56, Neural Cell Adhesion Molecule, NCAM, clone 123C3 This antibody recognizes a transmembrane glycoprotein of 140 and 180 kD which has been identified as NCAM (Neural Cell Adhesion Module). It reacts with epitope 2 of the two major epitopes which have been defined for NCAM. Thsi antibody is primarily used for the study of small cell carcinoma. Detection of CD56 positive lymphomas.

 

At the international Workshop on SCLC antibodies this clone was categorised as cluster 1 antibody (Stahel et al 1994). Most importantly, Clone 123C3 can be used on routing formalin fixed paraffin sections after retrieval and it stains all cells in small cell carcinomas and carcinoids of the lung strongly positive. Neuroblastoma's, wilms tumors and adenoid cystic carcinomas of bronchial glands are also stained strongly positive.

 

In non small lung cell carcinomas, clone 123C3 staining has been associated with more advanced stage and a decreased survival after surgery (Kibbelaar et al. 1991). Positive staining with other tumors, include medullary thyroid carcinomas and some ovarian tumors. NCAM (CD56) is a marker for natural killer cells and clone 123C3 can be used to distinguish NK cells from other haematopoietic cell populations. 

 

SCC Lung CD56

 

Mouse anti Keratin 7, clone OVTL12/30 This antibody reacts specifically with Keratin 7. It stains a 54 kD protein band of cytoskeletal proteins from cell lines in one and two dimensional immuno blots. It cross reacts with human and mouse. The antibody is a useful marker for histopathology and can be used to distinguish between ovarian carcinomas and colon carcinomas in paraffin embedded tissue sections.

 

 CK 7 Staining

 

Mouse anti Eosinophil Major Basic Protein, clone BMK-13 This antibody binds to the 10 kD eosinophil Major Basic Protein (MBP) of both resting and activated eosinophils in cytospins and frozen sections of bronchial and skin biopsies of allergic sites and normal sites and thus can be used as a "pan - eosinophil" marker. It stains frozen sections of bronchial biopsies from atopic asthmatics, rhinitics and normal non-atopic subjects. It does not cross - react with any other human protein or cell.

 

This mAb is a very useful tool for clinical and experimental allergy studies. Clone BMK-13 is a pan-eosinophil marker in both frozen sections and paraffin embedded tissues. Also this antibody can be used in specific ELISA tests to determine the release of MBP and to quantify the number of eosinophils in various in vitro adhesion assays.

 



Breast Cancer Drug Resistance Antibodies 

 

Tumour cell insensitivity to anticancer drugs frequently appears as multidrug resistance (MDR), associated with the overexpression of one or more of a set of at least 10 different molecules, causing reduced drug levels at the intracellular target sites. These overexpressed molecules include transmembrane transporter proteins such as P glycoprotein, MRP1-9 and BCRP. In addition, the lung-resistance protein, recently identified as the major vault protein, has also been associated with MDR.


Our Monosan monoclonal antibodies specifically recognise these proteins and so can be used to help identify their roles in clinical drug resistance and explore their occurrence in normal human tissues and physiology. 

 

We highlight 4 of our Multi Drug Resistance Antibodies below, followed by a list all of the available antibodies in our Multi Drug Resistance range.

 

Anti Breast Cancer Resistance Protein (ABCG2) Clone BXP-21. This monoclonal antibody reacts with an internal epitope of BCRP, a 70 kD transmembrane half-transporter which is involved in Multidrug resistance. BXP-21 did not cross-react with the human MDR1, MRP1or MRP2 gene products during testing.

 

BXP21_VUMC 1_200_200x

 

Anti MDR3 P-gp (ABCB4) Clone P3II-26. This monoclonal antibody reacts with an internal epitope of MDR3 P-gp (a fragment of MDR3 P-gp comprising amino acid 629 - 692). P3II-26 does not cross-react with human MDR1 P-gp.

 

Anti MRP2 (ABCC2) Clone M2III-6. This monoclonal antibody reacts with an internal epitope of cMOAT/MRP2, a 170-180 kD transmembrane protein known as the canalicular multi-organic anion transporter, absent in patients with the Dubin-Johnson syndrome, an autosomal recessive liver disorder characterised by chronic conjugated hyperbilirubinemia. cMOAT/MRP2 is closely related to the multidrug resistance related protein MRP, and its overexpression has been observed in a subset of cisplatin resistant cell lines. Clone M2III-6 did not cross react with the human MDR1, MRP1, MRP3 and MRP5 gene products.

 

Follow the links below for further details of the other Multi Drug Resistance Antibodies Available and datsheets.

 

 anti MDR3 P-glycoprotein      anti MRP6 (ABCC6) 
 anti MRP1 (ABCC1)      anti MRP7 (ABCC10)
 anti MRP2 (ABCC2)      anti MRP8 (ABCC11)  
 anti MRP3 (ABCC3)      anti MRP9 (ABCC12)
 anti MRP4 (ABCC4)      anti BCRP (ABCG2)
 anti MRP5 (ABCC5)      anti MVP / LRP
       anti VPARP

 

 

 

 

 

 

 

 

 

Or if you have any questions simply use our contact form and we'll be happy to help.