Biosensis


Antibodies, kits and reagents for neuroscience research



Biosensis - fine bioscience tools

 

 

 

 

 

Biosensis specialises in antibodies and reagents for Neuroscience, with particular emphasis on neurotrophins and neurotrophin receptors. In addition to neurotrophins, the Biosensis neuroscience portfolio includes products for research in neurodegenerative diseases, neurodevelopment, and neuro-metabolism.

 

Areas of focus include Alzheimer's, Parkinson's and ALS diseases, as well as autophagy and metabolic and stress disorders including metabolic syndrome research, obesity, and neuroimmunology and inflammation.

 

The Biosensis’ range includes neuroscience research antibodies, more than 200 quantitative ELISA kits, tissue staining and cell visualization reagents, and purified proteins targeted for neuroscience and cellular disease research, many having been used in peer reviewed research publications.

 

Find your product using the search box above or if you have any questions please contact us as detailed on our contact and ordering page.

 


 


Biosensis’ rabbit and chicken antibodies to parvalbumin and human calretinin.

 

Interneurons represent a mixture of diverse neuronal sub-populations, and thus, antibodies to specific cell markers are important to differentiate interneuron cell types. Biosensis’ new mouse, rabbit and chicken antibodies to human calretinin (M-1799-100, R-1800-50, C-1801-50) and parvalbumin (M-1813-100, C-1814-50) are high-quality research reagents for immunohistochemical and western blotting studies to identify the physiological role of neuronal sub-populations.

 

 mouse anti-parvalbumin staining

  

Left: Rat cerebellum section stained with mouse anti-parvalbumin (M-1813-100, green) and chicken anti-calbindin (C-1798-50, red) by Immunohistochemistry. Blue: DAPI nuclear stain. Most Purkinje cells strongly express both parvalbumin and calbindin and thus appear yellow. Basket, stellate and Golgi cells express parvalbumin alone, and thus appear green.

 

Right: Detection of calretinin (29 kDa) by Western Blotting with rabbit anti-calretinin (R-1800-50) in (2) rat brain, (3) rat spinal cord, (4) mouse brain, (5) mouse spinal cord, and (6) cow spinal cord homogenates.

 

Interested in other neuronal marker antibodies?

Biosensis neuronal marker antibodies or our full range of antibodies to calretinin, or our parvalbumin antibodies.

 



Biosensis c-Fos antibodies

The transcription factor c-Fos remains one of the most important neuronal activity markers. As c-Fos is frequently used in multiple IHC staining, Biosensis offers high quality c-Fos antibodies made in mouse (M-1752-100), rabbit (R-1751-50) and sheep (S-045-50).

c-Fos antibody staining

A: c-Fos expression in serum-starved C6 cells, stimulated by 20% FBS. B: Detection of c-Fos expression with sheep anti-c-Fos antibody (S-045-50) in rat hypothalamic arcuate nucleus, stimulated by N-methyl-D-aspartate. C: c-Fos activity (red) detected in mouse hippocampus with rabbit anti-c-Fos (R-1751-50). Green: NF-L-IR.

We also have other c-Fos antibodies available.

 



Novel antibody for p75(NTR) research

Our new p75NTR antibody (clone 8J2) to the extracellular domain of p75NTR (M-1818-100) is ideal for:

· In vivo targeting: retrograde tracing and receptor trafficking

· Flow cytometry, immunopanning, western blot, immuohistochemistry and more

· Most mammalian species, including human, mouse and rat

· Replacing clone MLR2

 

Comparison of clones 8J2 and MLR2

 

8J2 vs. MLR2

 

Top left and right images: Flow Cytometry analysis on human SHSY-5Y and rat C6 cell lines.

 

Above, Lower left image: Western blotting on human and rodent RIPA cell lysates: A: Mouse p75NTR-transfected cells. B: Non-transfected control cells. C: Human SH-SY5Y. D: Rat C6.

 

Above, Lower right image: Immunoprecipitation from rat C6 cells with clones 8J2 and MLR2. A: Precipitated p75NTR. B: Remaining supernatant. C: Protein G agarose only control.

 

Immunocytochemistry Comparison of clones 8J2 and MLR2

 

Images above: Analysis of p75NTR expression in non-permeabilized rat C6 cells by Immunocytochemistry. Both antibodies reveal punctuate membrane staining of p75NTR receptor. Magnification: 100x.



Biosensis MOAB-2 antibody to Amyloid beta peptide (40/42)

Our Biosensis MOAB-2 antibody (M-1586-100) is a new pan-specific monoclonal antibody to Amyloid beta residues 1-4 and provides unparalleled staining clarity, stronger reactivity and greater sensitivity than any other Abeta monoclonal antibodies previously available for research. Also, unlike other monoclonals such as 6E10 and 4G8, MOAB-2 does not cross react with APP or APP C-terminal fragments.

Biosensis' formulation of MOAB-2 is also the only one endorsed by the original developers.

 

Biosensis MOAB-2 antibody to Amyloid beta peptide (A beta 40/42)

Photo courtesy of Dr. Mary Jo LaDu and Dr. Leon Tai from the University of Illinois Chicago, Chicago, IL.

 

IHC staining of MOAB-2 on 2,4 and 6 month old 5XFAD mouse hippocampal sections showing increasing Aβ staining with MOAB-2 (Red); NeuN staining (Green). 

 



Amylo-Glo - Amyloid beta plaque staining

 

Amylo-Glo RTD is an excellent marker that shows clear superiority for visualising amyloid plaques over conventional markers. Its unique emission/excitation profile, high brightness and mild conditions make it suitable for multi-labelling and low magnification studies on tissues and cytochemistry.

 

It is also available with an EtBr counter stain allowing the assessment of amyloid plaques and cell/tissue positioning in one step.

 

To find out more please follow the link above

Amylo glo