Exosome and Microvesicle Research
Exosome isolation and quantification, exosome standards, antibodies and RNA extraction
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Our exosome and microvesicle research range provides essential tools for researchers in the field of exosome research from the HansaBioMed range including the ExoTEST platform, a proprietary immune-based solution for the capture, quantification and characterisation of exosomes from biological fluids and EXO-Prep an efficient method for exosome isolation.
Also we represent the exosomics range which provides solutions for the isolation and selective enrichment of tumour-originated extracellular vesicles (EVs) and exosomes from complex biofluids. Further information is provided below.
If you are looking for a specific product from the HansaBioMed range please follow the 'essential tools' link above for further details, pricing and copies of manuals and datasheets, or if you would like to request more information, simply use our contact form and we'll send you the information you need.
More information on exosome research including highlighted papers and other resources is available on our Exosome Twitter feed: Tweets by NKTSciExosome
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The exosomics range provides solutions for the isolation and selective enrichment of tumour-originated extracellular vesicles (EVs) and exosomes from complex biofluids:
Exosomics’ first two kits, SeleCTEV-DNA and SoRTEV-RNA, overcome the limitations of current liquid biopsy methods that isolate total nucleic acids in blood but with only low levels of tumour-derived RNA and DNA. On the contrary, the Exosomics kits allow the harvest of tumour-derived genetic material using unique proprietary affinity methods that selectively purify tumour-derived EVs and exosomes without using any special equipment, such as ultracentrifugation or chromatography and from which the DNA or RNA can be extracted. These kits hence provide researchers invaluable solutions to detect early cancer hallmarks in blood samples.
Purification of tumour-originated nucleic acids from tumour enriched EVs and exosomes
SoRTEV™ EV-RNA Low Volume (24 rxns) This kit allows the purification of tumour derived RNA from tumour enriched exosomes in biofluids and includes a user-friendly protocol for RNA purification. The exosome purification is based on immune-affinity beads coated with proprietary antibodies against exosome surface antigens.
SeleCTEV™ EV-DNA Low Volume (24rxn) This kit allows the purification of both circulating free DNA (cfDNA) and tumour-derived exosome DNA from plasma and/or serum with a starting volume of 0.5 to 2ml. The purification is based on Exosomics proprietary peptide affinity method and this kit includes a user-friendly protocol for DNA purification.
SeleCTEV™ EV-DNA High Volume (24rxn) This kit allows the purification of both circulating free DNA (cfDNA) and tumor-derived exosome DNA from plasma and/or serum with a starting volume of 2 to 7 ml. The purification is based on Exosomics proprietary peptide affinity method and this kit includes a user-friendly protocol for DNA purification.
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Save time when purifying microvesicles with the TFF-MV Concentrator
HansaBioMed’s new concentrator TFF-MV allows the one-step purification of microvesicles from large volume samples from 10mL to several litres.
The filter cartridge is made of polysulfone hollow fibers and can separate different extracellular vesicles (EVs) by size. Water, small molecules and small EVs pass through the hollow fibre pores, only leaving the microvesicles larger than 150 nm retained in the hollow fibre cartridge. The larger microvesicles can then be retrieved with a syringe in PBS buffer and do not require any further purification steps. Smaller EVS can also be recovered from the collection bag used during the process and purified further using for instance size exclusion chromatography columns. TFF-MV is based on tangential flow filtration methodology to prevent the clogging of the membrane of the filter and the concentrator can be reused up to 20 times.
Related products: HansaBioMed Pure-EV Size exclusion columns
Blog: Concentrating exosome samples with a tangential flow filtration system
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EXO-Prep is a NEW and efficient method for exosome isolation from biofluids (i.e plasma serum and urine) or cell culture supernatant.
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