Newmarket Scientific
Newmarket Scientific








Highlighted Antibody - HER2 (ERBB2) Clone BSR44



HER2/ERBB2, is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and gastric tumors. Immunohistochemical staining of HER-2 protein is graded as 0/1+, 2+ and 3+.

 

This antibody provides truly optimal staining and as with all our antibodies we provide a 100% guarantee, so trying it really is risk free.

 

Suggested Protocol after paraffin removal and rehydration:

 

1. Pretreatment: HIER pH9

2. Wash (TBS-Tween)

3. Primary antibody: HER2 1:100 – 1:300, 30 min.

4. Wash

5. 3% H2O2, 10 min 

6. Wash

7. HRP One-Step Polymer, 30 min

8. Wash

9. Wash

10. DAB high contrast Kit, 10 min

11. Aqua

12. CuSO4 -post enhancement, 5 min

13. Aqua

14. Counter staining in diluted Mayer, 1 min

15. Bluing, 7 min in tap water

16. Dehydration, clearing and mounting

 

Optibodies™ are mouse and rabbit monoclonal antibodies that have been developed specifically for use in immunohistochemistry to have high affinity and high specificity to their target antigens, give a high signal to noise ratio and can be used to achieve an intensive and specific staining.

 

Anti-HER2 (Clone BSR44)  HER2-Datasheet

 

Please visit our optibodies page and follow the individual product links for more information about the full optibodies range.

 


 

Optimal results for Optibodies™ in External QC assessments!

 

In recent quality assessments by External QC service providers, optimal staining results were obtained for SEVEN different Optibodies clones:

SOX10 - Transcription factor SOX-10 (Clone BS7)

CK5 - Cytokeratin 5 (Clone BSR55)

HER2 - IHC (Clone BSR44)

EpCAM - Epithelial cell-cell adhesion molecule (Clone BS14)

SMA - Alpha-Smooth Muscle Actin (Clone BS66)

MLA - Melan A (Clone BS52)

PDL-1 - Programmed Death Ligand 1 (Clone BSR90)

 



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