Newmarket Scientific
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ImmunoStar 24287 100 uL £351.00
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Product Detail: nNOS:C-Terminal (neuronal Nitric Oxide Synthase) Antibody
Background Info: Raised against a C-terminal synthetic peptide sequence corresponding to amino acids 1419-1433 of human nNOS coupled to KLH.
Product Type: Antibody - Antibodies
Antibody Type: polyclonal
Format: Lyophilised
Host Animal: Rabbit
Species Reactivity: Cat, Chick, Ferret, Guinea Pig, Hamster, Human, Monkey, Mouse, Opossum, Rat, Shrew, Squirrel Monkey, Vole, Zebrafish
Immunogen: Human nNOS (1419-1433)
Applications: Immunohistochemistry, Immunocytochemistry, immunofluorescence, Western Blot
Additional Info: The neuronal nitric oxide synthase C-terminal antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus, striatum, cortex and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/1,000 - 1/1,500 in PBS/0.3% Triton X-100 - Cy3 Technique and 1/8,000 - 1/12,000 in PBS/0.3% Triton X-100 - Bn/Av-HRP Technique. By Western blot analysis of brain homogenates the antibody specifically labels a band of approximately 155 kD. Immuno-labeling is completely abolished by pre-adsorption with synthetic human nNOS (1419-1433) at 5 µg per mL of diluted antibody. No cross reactivity with other forms of NOS were observed. The nNOS antiserum has been used successfully in human, rat, mouse, guinea pig, cat, and monkey tissue. Detection of nNOS from other species will depend upon sequence homology.
PubMed IDs: 10068309, 9459447, 9096405, 8393637, 7534654, 7528096, 7516501, 7094343, 1474363, 24994982
Product category: Aging, Alzheimer's, Apotosis, Cancer, DNA, Homeostasis, Huntington's, Hypoxia, Metabolic, Transcription Factor