The NEXTflex Methyl-Seq 1 Kit contains enough material to prepare 8 genomic DNA samples for Illumina compatible sequencing. The shelf life of all reagents is 12 months when stored properly. DNA Binding Buffer, DNA Wash Buffer and the Clean-Up Spin Columns should be stored at room temperature. All of the other components can be safely stored at -20°C.. The shelf life of all reagents is 12 months when stored properly at -20°C. Ships on dry ice.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
The NEXTflex Methyl-Seq 1 Kit is designed to enrich and prepare single, paired-end and multiplexed methylated DNA libraries for sequencing using Illumina MiSeq, GAIIx and HiSeq platforms. NEXTflex Methyl-Seq utilizes a versatile immune-capture (MeDIP) or methyl-CpG-binding domain (MeCAP) for detection of methylated DNA allowing the user to easily assess the methylation state of the genome.
This kit features Enhanced Adapter Ligation Technology resulting in library preps with a larger number of unique sequencing reads. This specially designed NEXTflex ligation enzymatic mix allows users to perform ligations with longer adapters and better ligation efficiencies. The NEXTflex Methyl-Seq 1 Kit simplifies workflow by using master mixed reagents and magnetic bead based cleanup, reducing pipetting and eliminating time consuming steps in library preparation. In addition, the availability of the NEXTflex DNA Barcodes with up to 96 unique adapter indices makes this the most high-throughput kit available.
For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
[KIT FEATURES] >>> Methylome-level assessment with broad genome coverage >>> Make methyl rich libraries using methylated DNA immunoprecipitation (MeDIP) or MBD capture (MeCAP) >>> Quantify absolute DNA methylation levels >>> Identify differentially methylated regions (DMRs) >>> Gel-Free and bead-based cleanup protocol replaces columns >>> Enhanced Adapter Ligation Technology with NEXTflex Ligation >>> Barcoded adapters for multiplexing contain embedded index sequence >>> Automation-friendly workflow is compatible with liquid handlers >>> Flexible barcode options kits containing 6, 12, 24, 48 and 96 unique barcodes >>> Functionally validated with Illumina GAIIx and HiSeq 2000 sequencing platforms
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 5118-01
The NEXTflex Methyl-Seq 1 Kit contains enough material to prepare 8 genomic DNA samples for Illumina compatible sequencing. The shelf life of all reagents is 12 months when stored properly. DNA Binding Buffer, DNA Wash Buffer and the Clean-Up Spin Columns should be stored at room temperature. All of the other components can be safely stored at -20°C.. The shelf life of all reagents is 12 months when stored properly at -20°C. Ships on dry ice.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
The NEXTflex Methyl-Seq 1 Kit is designed to enrich and prepare single, paired-end and multiplexed methylated DNA libraries for sequencing using Illumina MiSeq, GAIIx and HiSeq platforms. NEXTflex Methyl-Seq utilizes a versatile immune-capture (MeDIP) or methyl-CpG-binding domain (MeCAP) for detection of methylated DNA allowing the user to easily assess the methylation state of the genome.
This kit features Enhanced Adapter Ligation Technology resulting in library preps with a larger number of unique sequencing reads. This specially designed NEXTflex ligation enzymatic mix allows users to perform ligations with longer adapters and better ligation efficiencies. The NEXTflex Methyl-Seq 1 Kit simplifies workflow by using master mixed reagents and magnetic bead based cleanup, reducing pipetting and eliminating time consuming steps in library preparation. In addition, the availability of the NEXTflex DNA Barcodes with up to 96 unique adapter indices makes this the most high-throughput kit available.
For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
[KIT FEATURES] >>> Methylome-level assessment with broad genome coverage >>> Make methyl rich libraries using methylated DNA immunoprecipitation (MeDIP) or MBD capture (MeCAP) >>> Quantify absolute DNA methylation levels >>> Identify differentially methylated regions (DMRs) >>> Gel-Free and bead-based cleanup protocol replaces columns >>> Enhanced Adapter Ligation Technology with NEXTflex Ligation >>> Barcoded adapters for multiplexing contain embedded index sequence >>> Automation-friendly workflow is compatible with liquid handlers >>> Flexible barcode options kits containing 6, 12, 24, 48 and 96 unique barcodes >>> Functionally validated with Illumina GAIIx and HiSeq 2000 sequencing platforms
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 5118-02
The NEXTflex DNA Barcodes Kits contains 6, 12, 24 or 48 unique barcodes, enabling the user to multiplex up to 48 samples per flow cell lane. These kits ship on dry ice. These barcodes can be used with DNA-Seq kits, Rapid DNA-Seq kits and the Methyl-Seq kits.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Rapid DNA, Methyl-Seq, SeqCap EZ Target Capture, SureSelect, Cell Free DNA-Seq Kit for Illumina, barcode blockers
Additional Info:
NEXTflex DNA Barcodes can be used to provide flexibility and high-throughput capabilities in sequencing applications. They significantly increase scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. The NEXTflex DNA Barcodes kits accomplish this by using an indexed adapter with a 6 nt unique sequence. This allows for proper differentiation between samples, preventing poor reads from single base errors introduced during PCR. The NEXTflex index is contained within the adapter sequence eliminating the need to perform PCR to add flow cell binding sequences. These barcodes can be used with single, paired-end and multiplex reads and are compatible with the NEXTflex DNA Sequencing kits and PCR-free sequencing workflows and the NEXTflex Rapid DNA-Seq Kit.
For larger volume requirements, customized and bulk packaging is available.
[BENEFITS] >>> 6 nt index contained within adapter sequence eliminates the need to perform PCR to add flow cell binding sequences >>> Up to 48 multiplexed samples (up to 384 reactions per kit) >>> Compatible with PCR-free sequencing workflows >>> Considerably reduce your per-sample sequencing cost by barcoded multiplexing >>> Increase your sequencing scale by pooling 100s of samples on a single flow cell >>> Compatible with Illumina Next-Generation Sequencing platforms (GAII and HiSeq 2000)
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 514101
The NEXTflex DNA Barcodes Kits contains 6, 12, 24 or 48 unique barcodes, enabling the user to multiplex up to 48 samples per flow cell lane. These kits ship on dry ice. These barcodes can be used with DNA-Seq kits, Rapid DNA-Seq kits and the Methyl-Seq kits.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Rapid DNA, Methyl-Seq, SeqCap EZ Target Capture, SureSelect, Cell Free DNA-Seq Kit for Illumina, barcode blockers
Additional Info:
NEXTflex DNA Barcodes can be used to provide flexibility and high-throughput capabilities in sequencing applications. They significantly increase scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. The NEXTflex DNA Barcodes kits accomplish this by using an indexed adapter with a 6 nt unique sequence. This allows for proper differentiation between samples, preventing poor reads from single base errors introduced during PCR. The NEXTflex index is contained within the adapter sequence eliminating the need to perform PCR to add flow cell binding sequences. These barcodes can be used with single, paired-end and multiplex reads and are compatible with the NEXTflex DNA Sequencing kits and PCR-free sequencing workflows and the NEXTflex Rapid DNA-Seq Kit.
For larger volume requirements, customized and bulk packaging is available.
[BENEFITS] >>> 6 nt index contained within adapter sequence eliminates the need to perform PCR to add flow cell binding sequences >>> Up to 48 multiplexed samples (up to 384 reactions per kit) >>> Compatible with PCR-free sequencing workflows >>> Considerably reduce your per-sample sequencing cost by barcoded multiplexing >>> Increase your sequencing scale by pooling 100s of samples on a single flow cell >>> Compatible with Illumina Next-Generation Sequencing platforms (GAII and HiSeq 2000)
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 514102
The NEXTflex DNA Barcodes Kits contains 6, 12, 24 or 48 unique barcodes, enabling the user to multiplex up to 48 samples per flow cell lane. These kits ship on dry ice. These barcodes can be used with DNA-Seq kits, Rapid DNA-Seq kits and the Methyl-Seq kits.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Rapid DNA, Methyl-Seq, SeqCap EZ Target Capture, SureSelect, Cell Free DNA-Seq Kit for Illumina, barcode blockers
Additional Info:
NEXTflex DNA Barcodes can be used to provide flexibility and high-throughput capabilities in sequencing applications. They significantly increase scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. The NEXTflex DNA Barcodes kits accomplish this by using an indexed adapter with a 6 nt unique sequence. This allows for proper differentiation between samples, preventing poor reads from single base errors introduced during PCR. The NEXTflex index is contained within the adapter sequence eliminating the need to perform PCR to add flow cell binding sequences. These barcodes can be used with single, paired-end and multiplex reads and are compatible with the NEXTflex DNA Sequencing kits and PCR-free sequencing workflows and the NEXTflex Rapid DNA-Seq Kit.
For larger volume requirements, customized and bulk packaging is available.
[BENEFITS] >>> 6 nt index contained within adapter sequence eliminates the need to perform PCR to add flow cell binding sequences >>> Up to 48 multiplexed samples (up to 384 reactions per kit) >>> Compatible with PCR-free sequencing workflows >>> Considerably reduce your per-sample sequencing cost by barcoded multiplexing >>> Increase your sequencing scale by pooling 100s of samples on a single flow cell >>> Compatible with Illumina Next-Generation Sequencing platforms (GAII and HiSeq 2000)
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 514103
The NEXTflex DNA Barcodes Kits contains 6, 12, 24 or 48 unique barcodes, enabling the user to multiplex up to 48 samples per flow cell lane. These kits ship on dry ice. These barcodes can be used with DNA-Seq kits, Rapid DNA-Seq kits and the Methyl-Seq kits.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Rapid DNA, Methyl-Seq, SeqCap EZ Target Capture, SureSelect, Cell Free DNA-Seq Kit for Illumina, barcode blockers
Additional Info:
NEXTflex DNA Barcodes can be used to provide flexibility and high-throughput capabilities in sequencing applications. They significantly increase scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. The NEXTflex DNA Barcodes kits accomplish this by using an indexed adapter with a 6 nt unique sequence. This allows for proper differentiation between samples, preventing poor reads from single base errors introduced during PCR. The NEXTflex index is contained within the adapter sequence eliminating the need to perform PCR to add flow cell binding sequences. These barcodes can be used with single, paired-end and multiplex reads and are compatible with the NEXTflex DNA Sequencing kits and PCR-free sequencing workflows and the NEXTflex Rapid DNA-Seq Kit.
For larger volume requirements, customized and bulk packaging is available.
[BENEFITS] >>> 6 nt index contained within adapter sequence eliminates the need to perform PCR to add flow cell binding sequences >>> Up to 48 multiplexed samples (up to 384 reactions per kit) >>> Compatible with PCR-free sequencing workflows >>> Considerably reduce your per-sample sequencing cost by barcoded multiplexing >>> Increase your sequencing scale by pooling 100s of samples on a single flow cell >>> Compatible with Illumina Next-Generation Sequencing platforms (GAII and HiSeq 2000)
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 514104
The NEXTflex-96 DNA Barcodes can be used to provide flexibility and high-throughput capabilities in sequencing applications. They significantly increase scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. The NEXTflex-96 DNA Barcodes kits accomplish this by using an indexed adapter with an 8 nt unique sequence. This allows for proper differentiation between samples, preventing poor reads from single base errors introduced during PCR. The NEXTflex index is contained within the adapter sequence eliminating the need to perform PCR to add flow cell binding sequences. The NEXTflex-96 DNA Barcode kits are supplied in either a 96-well format (cat # 514105) or in microfuge tubes (cat # 514106). Do you want to index you samples but dont want to use 96 different barcodes? We also offer the NEXTflex DNA Barcodes in sets of 6, 12, 24 and 48 barcodes. These barcodes can be used with DNA-Seq kits, Rapid DNA-Seq kits and the Methyl-Seq kits.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Rapid DNA, Methyl-Seq, SeqCap EZ Target Capture, SureSelect, Cell Free DNA-Seq Kit for Illumina, NEXTflex Pre-Capture Combo, barcode blockers
Additional Info:
The NEXTflex-96 DNA Barcodes can be used to provide flexibility and high-throughput capabilities in sequencing applications. They significantly increase scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. The NEXTflex-96 DNA Barcodes kits accomplish this by using an indexed adapter with an 8 nt unique sequence. This allows for proper differentiation between samples, preventing poor reads from single base errors introduced during PCR. The NEXTflex index is contained within the adapter sequence eliminating the need to perform PCR to add flow cell binding sequences. The NEXTflex-96 DNA Barcode kits are supplied in either a 96-well format (cat # 514105) or in microfuge tubes (cat # 514106). Do you want to index you samples but dont want to use 96 different barcodes? We also offer the NEXTflex DNA Barcodes in sets of 6, 12, 24 and 48 barcodes.
These barcodes can be used with single, paired-end and multiplex reads and are compatible with the NEXTflex DNA Sequencing kits, other genomic DNA library prep and mRNA-Seq protocols and the NEXTflex Rapid DNA-Seq Kit.
For larger volume requirements, customized and bulk packaging is available.
[BENEFITS] >>> 8 nt index contained within adapter sequence eliminates the need to perform PCR to add flow cell binding sequences >>> Each kit comes with 8 reactions each of 96 multiplexed barcodes (768 reactions per kit) >>> Compatible with all DNA-Seq and mRNA-Seq application >>> Considerably reduce your per-sample sequencing cost by barcoded multiplexing >>> Increase your sequencing scale by pooling 100s of samples on a single flow cell >>> Compatible with Illumina Next-Generation Sequencing platforms (GAII and HiSeq 1000/2000) >>> The kits are supplied in either a 96-well plate format or 96 microfuge tubes
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 514105
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