For the development of sandwich ELISA kit to measure mouse CXCL4/PF4 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Product Type:
Antibodies Primary
Storage Temp:
-20°C
Immunogen:
Expression system for standard: E.coli; Immunogen sequence: V30-S105
Applications:
ELISA
Additional Info:
For the development of sandwich ELISA kit to measure mouse CXCL4/PF4 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Biosite Brand:
BioSite ELISA
Species Reactivity:
mouse
Cross Reactivity:
There is no detectable cross-reactivity with other relevant proteins.
Mouse CRP / C Reactive Protein / PTX1 Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Crp in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Product Type:
Assay & Detection
Storage Temp:
-20°C
Immunogen:
Expression system for standard: NS0; Immunogen sequence: H20-S225
Applications:
ELISA
Additional Info:
The Quick ELISA kits, assay takes less than 1.5 hours. Detect Mouse Crp with <10pg/ml sensitivity Compatible samples: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up.
Biosite Brand:
BioSite ELISA
Species Reactivity:
mouse
Cross Reactivity:
There is no detectable cross-reactivity with other relevant proteins.
For the development of sandwich ELISA kit to measure mouse CCL20/MIP-3 alpha in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Product Type:
Antibodies Primary
Storage Temp:
-20°C
Immunogen:
Expression system for standard: E.coli; Immunogen sequence: A28-M97
Applications:
ELISA
Additional Info:
For the development of sandwich ELISA kit to measure mouse CCL20/MIP-3 alpha in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Biosite Brand:
BioSite ELISA
Species Reactivity:
mouse
Cross Reactivity:
There is no detectable cross-reactivity with other relevant proteins.
Expression system for standard: NS0; Immunogen sequence: H129-R247
Applications:
ELISA
Additional Info:
The Quick ELISA kits, assay takes less than 1.5 hours. Detect Mouse BDNF with <15pg/ml sensitivity Compatible samples: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up.
Biosite Brand:
BioSite ELISA
Species Reactivity:
mouse
Cross Reactivity:
There is no detectable cross-reactivity with other relevant proteins.
The ZAP70 (zeta-associated protein of 70 kDa) tyrosine kinase was identified as a tyrosine phosphoprotein that associates with TCR zeta subunit and undergoes tyrosine phosphorylation following TCR stimulation. ZAP70 is a Syk family tyrosine kinase primarily expressed in T and NK cells that plays an essential role in signaling through the TCR. TCR-mediated activation of T cells is crucial to the immune response. In humans, ZAP70 gene mutations resulting in lower ZAP70 protein expression levels or expression of catalytically inactive ZAP70 proteins, have been identified. ZAP70 deficiency results in the absence of mature CD8+ T cells and the prevention of TCR-mediated activation of CD4+ T cells, and it can lead to severe combined immunodeficiency.In patients with chronic lymphocytic leukemia (B-CLL), ZAP70 expression on B cell was shown to be correlated with disease progression and survival. ZAP70 contains two N-terminal SH2 domains (Src homology domain 2) and a C-terminal kinase domain. During T cell activation, the binding of ZAP70 SH2 domains to the phosphorylated zeta subunit on the activated TCR complex causes a colocalization with the Lck tyrosine kinase that phosphorylates ZAP70 on Tyr493 in the activation loop. ZAP70 autophosphorylates multiple tyrosines in the region between the SH2 domains and the kinase domain, including the binding sites for additional SH2-containing signaling proteins such as SLP76, LAT, Lck, PLCgamma1, Vav, Shc, Ras-GAP, and Abl. ZAP70-mediated activation of these downstream effectors leads to the release of intracellular calcium stores, and the transcription of interleukin-2 and other genes important for an immune response.SpecificityApplication detailsFlow cytometry: Intracellular staining; recommended dilution: 2-5 ?g/ml; positive control: HPB-ALL human peripheral blood T cell leukemia cell line. <br>Western blotting: Recommended dilution: 0.5-2 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
ZAP-03
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
The Yes-associated protein, otherwise known as YAP, is a 14-3-3 binding molecule that was originally recognized by virtue of its ability to bind to the SH3 domain of Yes. The binding of YAP to 14-3-3 requires the phosphorylation of a homologous serine residue (Ser 112) in the YAP 14-3-3 binding motif. The highly conserved and ubiquitously expressed 14-3-3 proteins regulate differentiation, cell cycle progression and apoptosis by binding intracellular phosphoproteins involved in signal transduction. YAP may link events at the plasma membrane and cytoskeleton to inhibition of transcription in the nucleus in a manner regulated by 14-3-3 proteins. YAP shares homology with the WW domain of TAZ, transcriptional co-activator with PDZ binding motif, which functions as a transcriptional co-activator by binding to the PPXY motif present in transcription factors. YAP is expressed at high levels in the lung, placenta, prostate, ovary and testis. Pretreatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0, for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based detection is recommended. Control tissue Lung, placenta, prostate, ovary, testis. Staining Nuclear.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
63.7
Concentration:
n/a
Format:
Concentrate
Storage buffer:
PBS with less than 0.1% sodium azide and 0.1% gelatin
Storage:
2-8°C
References 1:
Ren, Y.R., et al. 2011. S, J. Biol. Chem. 286: 11960-11969.
References 2:
Ellison, D.W., et al. 2011. Acta Neuropathol. 121: 381-396.
References 3:
Cordenonsi, M., et al. 2011. Cell 147: 759-772.
References 4:
Ren, Y.R., et al. 2012. J. Proteome Res. 11: 5301-5310.
References 5:
Vigneron, A.M. and Vousden, K.H. 2012. EMBO J. 31: 471-480.
Wilms tumor 1 protein (WT1) is a zinc finger transcription factor, normally expressed in tissues of mesodermal origin. The Wilms tumor gene encodes a protein that functions as a tumor suppressor gene. WT1 is detected in tumor cells of Wilms Tumor (also known as nephroblastoma) and mesothelioma. Additionally, WT1 expression has been found in ovarian serous carcinomas and some breast carcinomas.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN Ready To Use
Clone:
6F-H2
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Charles AK; Moore IE; Berry PJ. Histopathology ; 30(4):312-4 (1997)
References 2:
Ordonez NG. Am J Surg Pathol 24(4):598-606, (2000)
References 3:
Foster MR, et al. Arch Pathol Lab Med; 125:1316-20 (2001)
References 4:
Nakatsuka S, et al. Mod Pathol; 19:804-14 (2006)
References 5:
May RJ, et al. Clin Cancer Res.; 13: 4547-55 (2007)
Wilms' tumor protein (WT1) has a role in transcriptional regulation and is expressed in the kidney and a subset of hematopoietic cells. Alteration of transcription factor function is a common mechanism in oncogenesis. The WT1 protein contains a DNA binding domain and any deletions or point mutations of the WT1 gene which destroy this activity result in the development of the childhood nephroblastoma Wilms' tumor and Denys-Drash syndrome. The description of WT1 involvement in nephroblastoma is not clear.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
WT49
Concentration:
Greater than or equal to 44 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Omeroglu A and Omeroglu G. Arch Pathol Lab Med. 2003; 127:e347-e348
References 2:
Lee SB and Haber DA. Experimental Cell Research. 2001; 264:74-99
The gene encoding WAF1, also termed p21, is transcriptionally regulated by the suppressor protein, p53. Overexpression of WAF1 is growth suppressive, possibly by inhibiting the activity of cyclin/CDK complexes. One consequence of WAF1 binding to cyclin/CDK is the inhibition of Rb protein phosphorylation. Induction of WAF1 expression requires wild type p53 activity in cells undergoing p53 dependent G1 arrest or apoptosis. Mutation of the p53 gene is a common event in human cancer and results in the failure to produce WAF1. The effect of this may lead to uncontrolled cell proliferation.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
4D10
Concentration:
n/a
Storage buffer:
Tissue culture supernatant with 15 mM sodium azide
Storage:
2-8°C
References 1:
Göhring UJ et al. Journal of Clinical Pathology. 54: 866870 (2001)
References 2:
Schwerer MJ et al. Journal of Clinical Pathology. 54: 871876 (2001)
References 3:
Tweddle DA et al. American Journal of Pathology. 158 (6): 20672077 (2001)
References 4:
Garcia JF et al. Histopathology. 30: 120125 (1997)
WA-1 reacts with human and other mammalian p21, a tumor suppressor protein, belonging to the CDI family. The intracellular protein p21 is a 21 kDa protein, also known as wild-type p53-activated fragment 1 (WAF1). It is an inhibitor of cyclin-dependent kinases (Cdks) and of proliferating-cell nuclear antigen (PCNA). It is induced by wild type p53, but not by mutated p53, by mezerein (anti-leukemic compound) and by interferon-ß. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma and thyroid carcinoma).
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
WA-1
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Kovaric, J. et al, Int. J. Oncol. 9(suppl.), 835 (1996)
Human von Willebrand factor (or factor VIII-related antigen) is a 270 kD multimeric plasma glycoprotein. It mediates platelet adhesion to injured vessel walls and serves as a carrier and stabilizer for coagulation factor VIII. The von Willebrand factor has functional binding domains to platelet glycoprotein Ib, glycoprotein Ib/IIIa, collagen and heparin. Von Willebrand factor is synthesized by endothelial cells and is reported to be expressed in a number of tumors of vascular origin.
Antibody Isotype:
IgG2a
Monosan Range:
MONXtra
Clone:
36B11
Concentration:
Greater than or equal to 21 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Zhang Y et al. Human Pathology. 2005; 36:797-805
References 2:
Su H et al. Proceedings of the National Academy of Sciences, USA. 2000; 97(25):13801-13806
Monoclonal antibody BV1 recognizes human vitronectin. Vitronectin is an abundant glycoprotein (~75 kDa), consisting of 459 amino acids. About one third of the protein molecular mass is composed of carbohydrates. Vitronectin is found in blood plasma and the extracellular matrix. Vitronectin is a multifunctional protein, since it promotes attachment and spreading of animal cells in vitro, it inhibits cytolysis by the complement C5b-9 complex, and it modulates antithrombin III-thrombin action in blood coagulation. The protein consists of three domains: the N-terminal Somatomedin B domain (1-39), a central domain with hemopexin homology (131-342) and a C-terminal domain (347-459) also with hemopexin homology. The Somatomedin B domain binds to Plasminogen Activator Inhibitor-1 (PAI-1) and is responsible for PAI-1 stabilization. Furthermore, the Somatomedin B domain can also interact with the urokinase plasminogen activator receptor (uPAR). Vitronectin-uPAR interaction is required and sufficient to initiate downstream changes in cell morphology, migration and signal transduction. High plasma levels of both PAI-1 and uPAR have been shown to correlate with a negative prognosis for cancer patients. Additionally, vitronectin is a component of platelets and is as such involved in hemostasis. Amino acid 45-47 (RGD) are capable of binding to membrane bound integrins, which serve to anchor cells to the extracellular matrix. Vitronectin in plasma is an inactive monomer form. In contrast, tissue vitronectin is an active multimeric form and is able to interact with various matrix ligands like proteoglycans and collagen. Mice with a genetic deletion of vitronectin show delayed wound healing, suggesting an important role of vitronectin in tissue remodeling after injury. The monoclonal antibody BV1 binds to soluble vitronectin as well as to membrane bound vitronectin.
Vitronectin, also known as serum spreading factor, S-protein and epibolin, is a glycoprotein present both in human plasma and serum. Vitronectin has been shown to modulate blood coagulation and complement-induced cytolysis. It is also variably present in diverse loose connective tissues, often in co-localization elastic fibrils.
Eukaryotic cells contain a number of types of cytoplasmic filamentous proteins, microtubule, microfilaments and intermediate-sized filaments (IF). Vimentin, a 57 kD protein that is an intermediate filament is reported to be expressed in most cells of mesenchymal origin, including fibroblasts, endothelial cells, smooth muscle, melanocytes as well as T and B lymphocytes.
Antibody Isotype:
IgG2a
Monosan Range:
MONXtra
Clone:
SRL33
Concentration:
Greater than or equal to 60 mg/L
Storage buffer:
Tissue culture supernatant with 15 mM sodium azide
Storage:
2-8°C
References 1:
Runembert I et al. Journal of Cell Science 115: 71324 (2002)
Vimentin (57 kDa) is the most ubiquituos intermediate filament protein and the first to be expressed during cell differentiation. All primitive cell types express vimentin but in most non-mesenchymal cells it is replaced by other intermediate filament proteins during differentiation. Vimentin is expressed in a wide variety of mesenchymal cell types - fibroblasts, endothelial cells etc., and in a number of other cell types derived from mesoderm, e.g., mesothelium and ovarian granulosa cells. In non-vascular smooth muscle cellsand striated muscle, vimentin is often replaced by desmin, however, during regeneration, vimentin is reexpressed. Cells of the lymfo-haemopoietic system (lymphocytes, macrophages etc.) also express vimentin, sometimes in scarce amounts. Vimentin is also found in mesoderm derived epithelia, e.g. kidney (Bowman capsule), endometrium and ovary (surface epithelium), in myoepithelial cells (breast, salivary and sweat glands), an in thyroid gland epithelium. In these cell types, as in mesothelial cells, vimentin is coexpressed with cytokeratin.Furthermore, vimentin is detected in many cells from the neural crest. Particularly melanocytes express abundant vimentin. In glial cells vimentin is coexpressed with Glial Fibrillary Acidic Protein (GFAP). Vimentin is present in many different neoplasms but is particulary expressed in those originated from mesenchymal cells. Sarcomas e.g., fibrosarcoma, malignt fibrous histiocytoma, angiosarcoma, and leio- and rhabdomyosarcoma, as well as lymphomas, malignant melanoma and schwannoma, are virtually always vimentin positive. Mesoderm derived carcinomas like renal cell carcinoma, adrenal cortical carcinoma and adenocarcinomas from endometrium and ovary usually express vimentin. Also thyroid carcinomas are vimentin positive. Any low differentiated carcinoma may express some vimentin. Vimentin is frequently included in the so-called primary panel (together with CD45, cytokeratin, and S-100 protein). Intense staining reaction for vimentin without coexpression of other intermediate filament proteins is strongly suggestive of a mesenchymal tumour or malignant melanoma.SpecificityThe antibody ZAP-03 reacts with ZAP70, a 70 kDa protein tyrosine kinase expressed in T and NK cells (intracellular antigen). ZAP70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products.Application detailsImmunocytochemistry: Staining technique: (a) fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. Positive control: 3T3 murine Swiss albino fibroblast cell line, RBL rat basophilic leukemia cell line. <br>Immunohistochemistry (paraffin sections): Recommended dilution: 5 ?g/ml, positive tissue: skin fibroblast.<br>Western blotting: Recommended dilution: 1-2 ?g/ml.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
VI-10
Concentration:
1 mg/ml
Format:
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Storage buffer:
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Vimentin (57 kDa) is the most ubiquituos intermediate filament protein and the first to be expressed during cell differentiation. All primitive cell types express vimentin but in most non-mesenchymal cells it is replaced by other intermediate filament proteins during differentiation. Vimentin is expressed in a wide variety of mesenchymal cell types - fibroblasts, endothelial cells etc., and in a number of other cell types derived from mesoderm, e.g., mesothelium and ovarian granulosa cells. In non-vascular smooth muscle cellsand striated muscle, vimentin is often replaced by desmin, however, during regeneration, vimentin is reexpressed. Cells of the lymfo-haemopoietic system (lymphocytes, macrophages etc.) also express vimentin, sometimes in scarce amounts. Vimentin is also found in mesoderm derived epithelia, e.g. kidney (Bowman capsule), endometrium and ovary (surface epithelium), in myoepithelial cells (breast, salivary and sweat glands), an in thyroid gland epithelium. In these cell types, as in mesothelial cells, vimentin is coexpressed with cytokeratin.Furthermore, vimentin is detected in many cells from the neural crest. Particularly melanocytes express abundant vimentin. In glial cells vimentin is coexpressed with Glial Fibrillary Acidic Protein (GFAP). Vimentin is present in many different neoplasms but is particulary expressed in those originated from mesenchymal cells. Sarcomas e.g., fibrosarcoma, malignt fibrous histiocytoma, angiosarcoma, and leio- and rhabdomyosarcoma, as well as lymphomas, malignant melanoma and schwannoma, are virtually always vimentin positive. Mesoderm derived carcinomas like renal cell carcinoma, adrenal cortical carcinoma and adenocarcinomas from endometrium and ovary usually express vimentin. Also thyroid carcinomas are vimentin positive. Any low differentiated carcinoma may express some vimentin. Vimentin is frequently included in the so-called primary panel (together with CD45, cytokeratin, and S-100 protein). Intense staining reaction for vimentin without coexpression of other intermediate filament proteins is strongly suggestive of a mesenchymal tumour or malignant melanoma.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
VI-RE/1
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
The antibody does stain all cells that contain vimentin. Only frozen section can be used. The antibody only stains the 57kDa vimentin band in immunoblots performed on a lysate of human lymphocytes.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
V9
Concentration:
25 ug/ml
Storage buffer:
Culture medium with 0.01M Sodium Azide
Storage:
2-8°C
References 1:
Muijen GNP van (1984) Am J Pathol 116, 363-369
References 2:
Debus E et al. (1984) Eur J Cell Biol 34, 137-143
References 3:
Muijen G van (1986) Exp Cell Res 162; 97-113
References 4:
Corver WE et al. (1994) Cytometry 15, 117-128
References 5:
Broek JCM van den et al. (2000) Appl Immunohis & Mol Morphology 8, 316-321
Eukaryotic cells contain a number of types of cytoplasmic filamentous proteins, microtubule, microfilaments and intermediate-sized filaments (IF). Vimentin, a 57 kD protein that is an intermediate filament is reported to be expressed in most cells of mesenchymal origin, including fibroblasts, endothelial cells, smooth muscle, melanocytes as well as T and B lymphocytes.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
V9
Concentration:
Greater than or equal to 16 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Colella R et al. American Journal of Surgical Pathology. 2010;34:10-17
References 2:
Gimelli S et al. Molecular Cancer. 2009;8:52
References 3:
Arnardottir S et al. Neurosurgery and Psychiatry. 2004;75(6):917-920
References 4:
Vargel I et al. American Journal of Medical Genetics. 2002; 109(1):22-35
References 5:
Osborn M et al. European Journal of Cell Biology. 1984; 34:137-143
Vimentin is a 57kD, class-III, intermediate filament found in a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin. The anti-vimentin immunohistochemical reagent is also useful as a tissue process control reagent.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN Ready To Use
Clone:
V9
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Ishii Y, et al. Clin Exp Immunol. 1984; 58:183-92
References 2:
Battifora H. Am J Clin Pathol. 1991; 96:669-71
References 3:
Takeyoshi I, et al. Hepatogastroenterology. 2000; 47:1611-4
References 4:
Yaziji H, et al. Int J Gynecol Pathol. 2001; 20:64-78
LN-6 reacts with vimentin, a 58kDa protein, and specifically with a non-hematopoietic epitope of vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Vimentin is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells, and endothelium. Antibody against vimentin is useful as part of an antibody panel for differential diagnosis of tumors of unknown origin. It does not react with leukocyte common antigen-positive tissues such as lymphomas and leukemias.
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
LN-6
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Stathopoulos, E, et al, J. Histochem. Cytochem. 37: 1363-1370 (1989)
Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognising cytokeratins, lymphoid markers, S100, desmin and neurofilaments.
Villin is an actin-binding glycoprotein that serves an important role in the maintenance of the microvilli brush border in gastrointestinal (GI) mucosal epithelium and its associated tumors. Recent immunohistochemical studies with villin have shown that villin is not only expressed in carcinomas of the gastrointestinal tract, but also in renal cell carcinomas, pancreatic carcinomas, endometrial carcinomas, as well as carcinomas of the ovary and lungs. In addition, positive villin expression may be seen in neuroendocrine/carcinoid tumors of the GI tract and lungs.
Antibody Isotype:
N/A
Monosan Range:
MONOSAN Ready To Use
Clone:
CWWB1
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Robert W. Werling et al. Am J Surg. Path.2003; 27(3):303-310
References 2:
Tamboli P. et al Arch Pathol Lab Med 2002;126:1057-1063
References 3:
Zhang P. J. et al. Arch Pathol Lab Med. 1999;123:812-816
References 4:
Nishizuka S et al. Cancer Res. 2003 Sep 1;63(17):5243-50
Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
VCP (valosin-containing protein), also known as p97, TERA, ALS14, IBMPFD, HEL-220, IBMPFD1, or HEL-S-70, is a member of a protein family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP is a structural protein that associates with clathrin and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. In sperm this intra-acrosomal protein can be used as a marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
Hs-14
Concentration:
1 mg/ml
Storage buffer:
Tris buffered saline (TBS) solution with 15 mM sodium azide
The monoclonal antibody 1G11B1 recognizes vascular cell adhesion molecule-1 (VCAM-1). VCAM-1 is a member of the immunoglobulin superfamily of adhesion molecules, which includes ICAMs, PECAM-s and MADCAM, and is involved in leukocyte-endothelial cell interactions. The immunoglobulin superfamily is a type I transmembrane protein characterized by extracellular immunoglobin domains, a transmembrane region and a cytoplasmic tail. They are essential for the development of the embryo and for immune and inflammatory responses. These transmembrane glycoproteins mediate cell interaction with, and adhesion to, other cells and the extracellular matrix. VCAM-1 contains six immunoglobulin domains of the H-type and interacts with VLA-4 expressed on leukocytes. Multiple adhesion molecules play a role in leukocyte recruitment. The process of migration of a leukocyte through the vascular endothelium consists of the following steps: leukocyte-endothelium interaction (first tethering and rolling and than adhesion) and transendothelial migration. VCAM-1 is almost not expressed under physiological conditions. However, under appropriate pro-inflammatory conditions where the endothelium is exposed to inflammatory cytokines such as tumour necrosis factor-? or IL-1b and becomes activated, VCAM-1 gene expression is rapid elevated by the vascular endothelium. There is also a soluble form of VCAM-1 which is angiogenic and chemotactic for endothelial cells. sVCAM-1 is up-regulated in several disease states (eg, myocardial infarction, type 2 diabetes mellitus, primary antiphospholipid syndrome, and rheumatoid arthritis).
VEGF-21 reacts with Vascular Endothelial Growth Factor, also known as Vascular Permeability Factor (VEGF/ VPF) and is the key mediator of angiogenesis. The MWs are 19- 22kDa (reducing) and 38kDa-44kDa (non-reducing). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis. In addition to endothelial cells, VEGF and VEGF receptors are expressed on numerous non-endothelial cells including tumor cells.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
VEGF-21
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Tischer E. et al. J. Biol. Chem 266: 11947-11954 (1991)
The monoclonal antibody BV9 binds to the extracellular domain (EC3-EC4) of human VE-cadherin (vascular endothelial cadherin). Endothelial cells control the passage of plasma constituents and circulating cells from blood to the underlying tissues. VE-cadherin is of vital importance for the maintenance and control of endothelial cell contacts. Mechanisms that regulate VE-cadherinmediated adhesion are important for the control of vascular permeability and leukocyte extravasation. VE-cadherin regulates various cellular processes such as cell proliferation and apoptosis and modulates vascular endothelial growth factor receptor functions. Therefore, VE-cadherin is also essential during embryonic angiogenesis. The specialized function of VE-cadherin is lost or impaired in several pathological conditions - including inflammation, sepsis, ischemia and diabetes - which leads to severe, and sometimes fatal, organ dysfunction. Furthermore, abnormal increase in vascular permeability is often observed in pathological conditions, such as tumor-induced angiogenesis, macular degeneration, allergy, and brain stroke.<br /> Endothelial permeability is regulated in part by the dynamic opening and closure of cell-cell adherent junctions. In vascular endothelium, adherent junctions are mainly composed of VE-cadherin, an adhesive receptor that is able to self-associate at endothelial cellcell contacts. VE-cadherin links endothelial cells together by homophilic interactions mediated by its extracellular part and associates intracellularly with the actin cytoskeleton via catenins. VE-cadherin belongs to the cadherin super-family of cellcell adhesion molecules, which are encoded by more than 200 genes in the human genome. Classical cadherins are Ca2+-dependent, homophilic, cell to cell adhesion molecules expressed in nearly all cells within solid tissues. Cadherins form a core adhesion complex that consists of a cadherin dimer, binding through its extracellular region to another dimer of cadherins expressed in adjacent cells, while its intracellular region is anchored to the plasma membrane and linked to the cytoskeleton. The VE-cadherin extracellular domain consists of five cadherin-type repeats, called EC (extracellular cadherin) domains that are bound together by calcium ions in a rod-like structure.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
D1f3
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Navarro; P et al. J Biol Chem 1995; 270: 30965
References 2:
Martin-Padura, I et al J path 1995, 175: 51
References 3:
Breviario; F et al. Arterioscler Thromb 1995; 15: 1229-
Varicella Zoster Virus (VZV), a member of the human herpes virus family, causes two distinct clinical manifestations: chickenpox and shingles. Primary VZV infection results in chickenpox (varicella), which may rarely result in complications including encephalitis or pneumonia. Even when clinical symptoms of chickenpox have resolved, VZV remains dormant in the nervous system of the infected person (virus latency), in the trigeminal and dorsal root ganglia. In about 10-20% of cases, VZV reactivates later in life producing a disease known as herpes zoster or shingles. Serious complications of shingles include postherpetic neuralgia, zoster multiplex, myelitis, herpes ophthalmicus, or zoster sine herpete. VZV is closely related to the herpes simplex virus (HSV). Affected skin shares so many histological similarities that distinguishing between them may be difficult. Immunohistochemistry with anti-VZV appears quite sensitive and specific on formalin-fixed paraffin-embedded tissues in the distinction between HSV and VZV.
Antibody Isotype:
N/A
Monosan Range:
MONOSAN Ready To Use
Clone:
SG1-1, SG1-SG4, NCP-1 & IE-62 (7 clone cocktail)
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Kleinschmidt D, et al. J Neurol Sci. 1998 Aug 14; 159(2):213-8
References 2:
Kaye SB, et al. Br J Ophthalmol. 2000 Jun;84(6):563-71
References 3:
A.F. Nikkels, et al. Virchows Archiv A pathol Anat. 1993; 422:121-126
The utrophin gene is located on chromosome 6. Analyte Specific Reagent. Analytical and performance characteristics are not established. Amino terminal domain of the human homolog of human dystrophin, utrophin (also known as dystrophin related protein or DRP). Also crossreacts with utrophin in sections of muscle from rat and dog. Other animals species have not been tested.The product 2 is a lyophilized tissue culture supernatant containing sodium azide as a preservative. The user is required to reconstitute the contents of the vial with the correct volume of sterile distilled water as indicated on the vial label.
Uroplakins (UPs) are a family of transmembrane proteins (UPs Ia, Ib, II and III) that are specific differentiation products of urothelial cells. In non-neoplastic mammalian urothelium, UPs are expressed in the luminal surface plasmalemma of superficial (umbrella) cells, forming complexes of 16nm crystalline particles. UPIII is specific for tumors of urothelial origin and, when used in combination with other markers, can aid in the diagnosis of primary and metastatic tumors.
Uroplakins (UPs) are a family of transmembrane proteins (UPs Ia, Ib, II and III) that are specific differentiation products of urothelial cells. In non-neoplastic mammalian urothelium, UPs are expressed in the luminal surface plasmalemma of superficial (umbrella) cells, forming complexes of 16nm crystalline particles. UPIII is specific for tumors of urothelial origin and, when used in combination with other markers, can aid in the diagnosis of primary and metastatic tumors.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN Ready To Use
Clone:
AU-1
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Moll R, et al. Am J Pathol. 1995; 147:1383-97
References 2:
Olsburgh J, et al. J Pathol. 2003; 199:41-9
References 3:
Parker DC, et al. Am J Surg Pathol. 2003; 27:1-10
References 4:
Ohtsuka Y, et al. BJU Int. 2006; 97:1322-6
References 5:
Logani S, et al. Am J Surg Pathol. 2003 Nov;27(11):1434-41
Ubinuclein 1 (UBN1) is a ubiquitously expressed evolutionarily conserved protein which binds to proliferation-promoting genes that are repressed by formation of senescence-associated heterochromatin foci (SAHF). Ubinuclein 1 associates with various transcription factors and with histone methyltransferase activity, is indispensable for SAHF formation and appears to be a regulator of senescence. Although in most cells ubinuclein is localized to the nucleus, in cells forming tight junctions it is recruited to the cell adhesion complexes, dependently on the cell density.SpecificityThe antibody VI-10 reacts with vimentin, a 57 kDa intermediate filament intracellular protein expressed in variety of mesenchymal and mesodermal cell types.Application detailsWestern blotting: Recommended dilution: 1-5 ?g/ml; positive control: HeLa cell line.
Antibody Isotype:
IgG
Monosan Range:
MONOSAN
Clone:
UBN1-02
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
UACA (Uveal Autoantigen with Coiled-coil domains and Ankyrin repeats) is a 1,416 amino acid nuclear membrane protein. It was originally identified as an autoantigen in patients with panuveitis, a characteristic of Vogt-Koyanagi-Harada disease, and in patients with Graves' disease. UACA was also later identified as Nucling, a mRNA differentially expressed in F9 embryonal carcinoma cells, and that is up-regulated during cardiac muscle differentiation. UACA appears to function as a pro-apoptotic protein that recruits the apaf-1- pro-caspase-9 complex for the induction of apoptosis to mediate the cell-death pathway.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
AE-5
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Yamada, K., et al. Biochem. Biophys. Res. Commun. 280: 1169-1176 (2001)
The biosynthesis of melanin in melanocytes involves a family of enzymes, a key member of which is tyrosinase. Tyrosinase deficiency is associated with various forms of albinism and in particular oculocutaneous albinism. L-tyrosinase is the initial substrate for melanin biosynthesis and its conversion to dopaquinone is catalyzed by tyrosinase, whose expression is reported in melanocytes and melanomas.
Antibody Isotype:
IgG2a
Monosan Range:
MONXtra
Clone:
T311
Concentration:
Greater than or equal to 89 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Shidham VB et al. BMC Cancer. 2003; 3(1):15
References 2:
Lohmann CM et al. American Journal of Surgical Pathology. 2002; 26(10):13511357
References 3:
Clarkson KS et al. Journal of Clinical Pathology. 2001; 54(3):196200
References 4:
de Vries TJ et al. Journal of Pathology. 2001; 193(1):1320
References 5:
Jungbluth AA et al. Pathol Res Pract. 2000; 196(4):235242
Tyrosinase is an enzyme, amongst a family of enzymes, which is involved in the biosynthesis of melanin. It is a highly specific and sensitive marker for melanocytic differentiation, and has been found to be quite specific for melanotic lesions such as malignant melanoma.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN Ready To Use
Clone:
T311
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Kaufmann O, et al. Mod Pathol 1998 Aug; 11(8):740-6
References 2:
Meije CB; et al. J Pathol 2000 Apr; 190(5):572-8
References 3:
Kanitakis J et al. Am J Dermatopathol. 2002 Dec;24(6):498-501
References 4:
Eudy GE et al. Hum Pathol. 2003 Aug;34(8):797-802
References 5:
Jaanson N et al. Melanoma Res. 2003 Oct;13(5):473-82
The monoclonal antibody H398 recognizes the extracellular part of the Tumor Necrosis Factor Receptor type I (TNF-RI) of the membrane-bound as well as the soluble receptor. TNF-RI (~55-60 kDa) is present on most cell types and is considered to play a prominent role in cell stimulation by TNF-alpha. TNF-alpha activates inflammatory responses, induces apoptosis, regulates cellular proliferation, and may even promote cancer progression. The effects of TNF-alpha are mediated by TNF-RI and TNF-RII, which have both distinct and overlapping downstream signaling cascades. Induction of cytotoxicity and other functions are mediated largely via TNF-RI. TNF-RI is equally well activated by both the 17 kDa soluble and 26 kDa membrane-bound form, whereas TNF-RII is efficiently activated only by the membrane bound form of TNF-alpha. TNF-RI signaling is initiated when trimeric TNF-alpha binds TNF-RI receptors. Subsequent TNF-RI trimerization promotes the recruitment of a proximal signaling complex composed of TNF Receptor Associated protein with a Death Domain (TRADD), Receptor Interacting Protein (RIP), cellular Inhibitor of Apoptosis Protein 1 (cIAP1), TNF Receptor Associated Factor 2 (TRAF2), and likely TRAF5. Studies with TNF-RI-deficient mice indicate that TNF-RI mediates most of the proliferation, pro-inflammatory, and apoptosis-activating pathways.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
H398
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Thoma; B et al. J Exp Med 1990; 172: 1019
References 2:
Grell, M et al Lymphokine Cytokine Res 1993, 12: 143
References 3:
Scheurich; P et al. Tumor Necrosis factor 1993; 4: 52
References 4:
Grell M et al. Proc Natl Acad Sci USA 1998; 95: 570
References 5:
Krippner-Heidenreich A et al. J Immunol 2008; 180: 8176
The monoclonal antibody 52B83 reacts with tumor necrosis factor alpha (TNF-alpha). TNF-alpha is a homotrimeric 17 kDa protein, that interacts with either one of the two types of TNF-receptors, termed I and II, leading to receptor cross-linking and signal transduction. The receptors differ strongly in their intra-cellular signaling pathways.<br /> TNF-alpha was originally described as a highly cytotoxic cytokine for tumor cells, it causes tumor necrosis in vivo and shows cytolytic activity against tumor cells in vitro. Furthermore,- TNF-alpha is found to be a central mediator in many inflammatory and immunological processes. It can be induced by various products of micro-organisms and by various cytokines leading to expression of a wide variety of- cytokines. The pro-inflammatory properties of- TNF-alpha play a central role in several auto-immune diseases such as rheumatoid arthritis and inhibition by neutralizing molecules have been shown to be beneficial in patients.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
52B83
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Bradding; P et al. Am J Respir Cell Mol Biol 1994; 10: 471
References 2:
Bradding, P et al: Clin Exp Allergy 1995, 25: 406
References 3:
Gerspach; J et al. Microsc Res Tech 2000; 50: 243
References 4:
Laan van der N et al. Arch Dermatol Res 2001; 293: 226
TU-02 reacts with the N-terminal domain of alpha-tubulin. Tubulin isotypes have been identified with tissue specific expression. Immunocytochemical studies using several mAbs revealed remarkable heterogeneity of tubulin within various nervous tissues. TU-02 reacts with tubulin in fixed tissues only, not in unfixed or live tissues or cells. Interphase microtubules are also stained by TU-02 in fixed tissues.
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
TU-02
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Dráber, P. et. al. Eur.J.Cell.Biol. 41: 82-88 (1986)
References 2:
Dráber, P. et. al. Histochemistry 87: 151-155 (1987)
References 3:
Dráber, P. et. al. J. Cell Science 92: 519-528 (1989)
References 4:
Smertenko et al. Eur. J. Cell. Biol. 72: 104-112 (1997)
Anti-TTF-1 (Thyroid Transcription Factor 1) is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the organs rather than thyroid, germ cell tumors, and malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates. TTF-1 labeling is also seen in thyroid and thyroid-derived tumors. TTF-1 immunostaining is useful in the differentiation between pulmonary and nonpulmonary origin of adenocarcinomas in malignant effusions. TTF-1 staining is very reliable in discerning whether a brain metastasis has arisen from a pulmonary or nonpulmonary site, particularly when dealing with adenocarcinomas and largecell carcinomas.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN Ready To Use
Clone:
8G7G3/1
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Bejarano PA, et al. Mod Pathol. 1996; 9:445-52
References 2:
Di Loreto C, et al. Cancer Lett. 1998; 124:73-8
References 3:
Abutaily AS, et al. J Clin Pathol. 2002; 55:662-8
References 4:
Jang KY, et al. Anal Quant Cytol Histol. 2001; 23:400-4
Tryptases compose a subfamily of proteinases with trypsin-like activity that are mostly stored in mast cell secretory granules and released into the extracellular environment upon mast cell activation. Several biological functions for tryptases have been proposed, including involvement in inflammatory and allergic responses.1 Mature mast cells have a complex distribution throughout the body. Antitryptase is a useful marker for mast cells.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN Ready To Use
Clone:
G3
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Jordan JH et al. Hum Pathol. 2001 May;32(5):545-52
References 2:
Gordon LK et al. Clin Immunol. 2000 Jan;94(1):42-50
References 3:
Ghott A et al. Am J Surg Pathol. 2003 Jul;27(7):1013-9
References 4:
Aoki M et al. Int Arch Allergy Immunol. 2003 Mar;130(3):216-23
References 5:
Fiorucci L, et al. Cell Mol Life Sci. 2004 Jun;61(11):1278-95
Human transferrin receptor 1 (TfR1), also designated CD71, is a homodimeric type II membrane glycoprotein of 90-95 kDa. This receptor binds two molecules of the serum iron-transport protein transferrin (Tf) and is internalised into endosomes that are acidified, resulting in the release of iron from Tf. TfR1 is not expressed on resting leukocytes but is upregulated on all proliferating cells upon activation, reflecting the iron dependence of proliferation. In tissues TfR1 is expressed on most dividing cells and on brain capillary endothelium. Expression of TfR1 is down regulated as a result of iron overload. TfR1 shares 45% identity with TfR2.
Transferrin is a monomeric glycoprotein of approximately 77 kDa, which serves as an iron-transporter. In normal plasma, transferrin has a concentration of 25-50 µmol / liter, and is usually about one-third saturated with iron, thus providing a large buffering capacity in case of an acute increase in plasma iron levels. Cells take up transferrin-iron complexes (holotransferrin) using transferrin receptor dimers. Upon binding of holotransferrin, the receptor is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with its receptor (CD71) and recycles to the cell surface, where apotransferrin is released upon exposure to normal pH. Internalization of labeled transferrin thus represents an usefull approach to study endocytosis. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. Iron/transferrin complex is essential in haemoglobin synthesis and for certain types of cell division.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
HTF-14
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage:
2-8°C
References 1:
Bartek J., et al., Immunol. Lett. 1982; 7: 231.
References 2:
Bartek, J., et al., Br. J. Haematol 1985; 59: 435-441.
References 3:
Nováková, M., et al., Cell Motil Cytoskeleton 1996;33(1):38-51
TPX2 is a microtubule-associated protein, which is a critical regulator of mitosis. At the beginning of mitosis, TPX2 is released and plays a significant role in mitotic spindle formation and subsequent proper segregation of chromosomes during cell division. After completion of mitosis the TPX2 protein disappears, but has also role in DNA damage response. Its overexpression has been demonstrated in many types of carcinomas. TPX2 belongs to the markers of worse tumor prognosis. On the other hand, down-regulation of TPX2 can inhibit cancer cell proliferation, migration and invasion.SpecificityThe antibody UBN1-02 recognizes N-terminal part of ubinuclein 1 (UBN1), a widely expressed nuclear and adhesion complex protein. Western blotting analysis reveals UBN1 as a 165 kDa band.Application details
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
TPX2-01
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
The antibody MR2-1 reacts with the extra-cellular part of the TNF-RII. It also reacts with the soluble receptor. TNF-RII is present on most cell types and is considered to play a prominent role in cell stimulation by TNF-alpha. TNF-RII molecule is shown to be responsible for stimulation of activated T-lymphocytes by TNF-alpha. The antibody cross reacts with rhesus and cynomolgus natural TNF-RII.
TNF-alpha is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells. It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha easily forms stable trimers, but also other multimeric complexes. In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.SpecificityThe mouse monoclonal antibody TPX2-01 recognizes the epitope EPFVPKKEKKS (aa 636-646 in human) of TPX2, a microtubule-associated intracellular critical regulator of mitosis, which is overexpressed in many types of tumors and is a marker of worse prognosis in various cancers.Application detailsELISA: Biotinylated MAb11 can be used as a detection antibody in combination with capture antibody MAb1. <br>Immunohistochemistry (frozen sections): paraformaldehyde-fixed, saponin-treated frozen tissue sections. <br>Flow cytometry: Intracellular staining.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
MAb11
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
The antibody reacts with free soluble (17 kDa) and membrane (26 kDa) human TNF-alpha. The antibody inhibits the biological activity of soluble and membrane TNF-alpha. The antibody can be a useful tool to discriminate between receptor bound soluble (17 kDa) and the membrane (26 kDa) form of TNF-alpha. For this purpose we recommend to use this antibody in combination with the anti-TNF-alpha antibody HM2024, which recognizes only soluble and membrane TNF-alpha, but not the receptor bound TNF-alpha.
The antibody reacts with free soluble (17 kDa) and membrane (26 kDa) human TNF-alpha. The antibody inhibits the biological activity of both forms. It does not react with receptor bound TNF-alpha. It can be a useful tool to discriminate between the membrane form of TNF expressed on producer cells and the proteolytically cleaved, soluble TNF-alpha bound to its cognate cell membrane receptors (TNF-RI and TNF-RII). For this purpose we recommend to use this antibody in combination with the anti-TNF-alpha antibody HM2026, which recognizes soluble, membrane and receptor bound TNF-alpha.
The antibody reacts with human native and recombinant TNF-alpha as assessed by ELISA. The antibody inhibits the biological activity of human native and recombinant TNF-alpha as determined with L929 cells in a cytotoxicity assay. The antibody cross reacts with rhesus and cynomolgus natural TNF-alpha and lacks crossreactivity with human lymphotoxin.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
4H31
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Gerspach; J et al. Microsc Res Tech 2000; 50: 243
References 2:
Limb, GA et al Br J Ophthalmol 1996, 80: 168
References 3:
Laan van der; N et al. Arch Dermatol Res 2001; 293: 226
Tissue non-specific alkaline phosphatase (TNAP), also known as liver/bone/kidney alkaline phosphatase, or MSCA-1 (mesenchymal stem cell antigen 1) is a selective marker for the prospective isolation of bone marrow-derived mesenchymal stem cells and mesenchymal stem-like cells. It is expressed at high levels in liver, bone, kidney, or endometrium, as well as on embryonic stem cells (ESCs). TNAP also plays a role in bone mineralization. Mutations in TNAP gene are associated with hypercalcemia and skeletal defects (hypophosphatasia).SpecificityThe mouse monoclonal antibody MAb11 recognizes human 17-26 kDa cytokine TNF-alpha (tumor necrosis factor alpha).Application detailsFlow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
W8B2B10
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
The monoclonal antibody 5G5 reacts with the Toll-like receptor 9 (TLR9, CD289). TLRs are highly conserved throughout evolution and have been implicated in the innate defence to many pathogens. In Drosophila, toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defences. In mammals, TLRs identified as type I transmembrane signalling receptors with pattern recognition capabilities, have been implicated in the innate host defence to pathogens. As investigated so far all functional characterized TLR signal via the TLR/IL-1 receptor (IL-1R) pathway where recruitment of MyD88 seems to be essential. In contrast to cell-wall components, bacterial DNA is probably invisible for immune cells until DNA is liberated during processes taking place in the endosomal/lysosomal compartment where intracellular TLR9 recruits MyD88 to initiate signal transduction. Unmethylated CpG-dinucleotide-containing sequences are found much more frequently in bacterial genomes than in vertebrates genomes, whereas the frequency of CpG dinucleotides are suppressed and usually methylated. The regions adjacent to the CpG dinucleotides also affect the immunostimulatory activity. The optimal sequence differs significantly between mammalian species. Methylated CpG dinucleotides lack immunostimulatory activities. Cellular activation in response to bacterial DNA and synthetic dinucleotides containing unmethylated CpG-dinucleotides is mediated by TLR9. The monoclonal antibody 5G5 reacts with RAW macrophages and TLR9 transfected HEK293 cells, and it is cross reactive with canine TLR9.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
5G5
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Ahmad-Nejad; P et al. Eur J Immunol 2002; 32: 1958
The monoclonal antibody 5G5 recognizes human Toll-like receptor 9. Toll-like receptors (TLRs) are highly conserved from Drosophila to humans and share structural and functional similarities. TLRs constitute of a family of pattern recognition receptors (PRRs) that mediate cellular responses to a large variety of pathogens (viruses, bacteria, and parasites) by specific recognition of so-called âpathogen-associated molecular patternsâ. Activation of TLRs, a family of at least 11 differentmembers that function either as homo- or heterodimers, leads to activation of NFκB-dependent and IFNregulatory factor-dependent signaling pathways. TLRs have a central role in innate immunity and are also required for the development of an adaptive immune response. TLRs are expressed by various cells of the immune system, such as macrophages and dendritic cells. They recognize and respond to molecules derived from bacterial, viral and fungal pathogens.<br /> Whereas most TLRs are expressed on the cell surface, TLR9 is expressed intracellularly within one or more endosomal compartments and recognizes nucleic acids. TLR9 detects a rather subtle difference in the DNA of vertebrates compared with that of pathogens. Vertebrate genomic DNAs have mostly methylated CpG dinucleotides where bacterial and viral DNAs have unmethylated CpG dinucleotides. TLR9 undergoes relocation from endoplasmic reticulum to CpG-ODN-containing endosomes. In these endosomes TLR9 becomes a functional receptor after proteolytic cleavage. TLR9 exists as a preformed homodimer and CpG-ODN binding promotes its conformational change, bringing the cytoplasmic TIR-like domains close to each other. This allows a recruitment of the key adapter protein MyD88 which initiates a signalling cascade. The only human immune cell types known to constitutively express TLR9 and to be activated by CpG ODN are pDCs and B cells. TLR9 triggering induces an activation phenotype in the B cells and pDCs, characterized by the expression of costimulatory molecules, resistance to apoptosis, and induces Th1-type immune response profiles.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
5G5
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Ahmad-Nejad; P et al. Eur J Immunol 2002; 32: 1958
Toll-like receptors (TLRs) are highly conserved from Drosophila to humans and share structural and functional similarities. TLRs constitute of a family of pattern recognition receptors (PRRs) that mediate cellular responses to a large variety of pathogens (viruses, bacteria, and parasites) by specific recognition of so-called âpathogen-associated molecular patternsâ. Activation of TLRs, a family of at least 11 different members that function either as homo- or heterodimers, leads to activation of NFκB-dependent and IFN-regulatory factor-dependent signaling pathways. TLRs have a central role in innate immunity and are also required for the development of an adaptive immune response. TLRs are expressed by various cells of the immune system, such as macrophages and dendritic cells. TLRs are class I receptors, with a single ?-helix that spans the cell membrane. They recognize and respond to molecules derived from bacterial, viral and fungal pathogens, such as lipopolysaccharide (LPS) from the outer membrane of Gram negative bacteria, peptidoglycan fragments from bacterial cell walls and single-stranded and double-stranded RNA from viruses. Toll-like receptor 4 (TLR4; CD284) has been identified, next to MD-2 and CD14, as a receptor that is central to the innate immune response to LPS of Gram-negative bacteria. TLR4 is unique among TLRs in its ability to activate two distinct signaling pathways; one pathway is activated by the adaptors TIRAP (Toll/interleukin-1- receptor (TIR)-domain-containing adaptor protein) and MyD88, which leads to the induction of proâinflammatory cytokines. The second pathway is activated by the adaptors TRIF (TIR-domaincontaining adaptor protein inducing interferonâβ) and TRAM (TRIFrelated adaptor molecule), which leads to the induction of type I interferons. The monoclonal antibody HTA125 is a TLR4 function-blocking antibody. HTA125 recognizes preferentially human TLR4 that is associated with MD-2.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
HTA125
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Shimazu; R et al. J Exp Med 1999; 189: 1777
References 2:
Tabeta, K et al Infect Immun 2000, 68: 3731
References 3:
Akashi; S et al. Biochem Biophys Res Commun 2000; 268: 172
The monoclonal antibody TLR3.7 recognizes the 116 kDa human Toll-like receptor 3 (TLR3, CD283). Toll-like receptors (TLRs) are highly conserved from Drosophila to humans and share structural and functional similarities. TLRs constitute of a family of pattern recognition receptors (PRRs) that mediate cellular responses to a large variety of pathogens (viruses, bacteria, and parasites) by specific recognition of so-called âpathogen-associated molecular patternsâ. Activation of TLRs, a family of at least 11 different members that function either as homo- or heterodimers, leads to activation of NFκBdependent and IFN-regulatory factor-dependent signaling pathways. TLRs have a central role in innate immunity and are also required for the development of an adaptive immune response. TLRs are expressed by various cells of the immune system, such as macrophages and dendritic cells. TLRs are class I receptors, with a single ?-helix that spans the cell membrane. They recognize and respond to molecules derived from bacterial, viral and fungal pathogens, such as lipopolysaccharide (LPS) from the outer membrane of Gram negative bacteria, peptidoglycan fragments from bacterial cell walls and single-stranded and double-stranded RNA from viruses.<br /> Some forms of RNA and DNA from pathogens exhibit immutable features that distinguish them from nucleic acids of higher organisms. For example, dsRNA, is a common intermediate of viral replication and a potent indicator of infection. Toll-like receptor 3 (TLR3) recognizes viral double-stranded RNA and its synthetic analog polyriboinosinic:polyribocytidylic acid (poly(I:C)). TLR3 is normally located in acidic endosomes where its luminal ectodomain (ECD) encounters dsRNA and induces type I interferon (IFN), inflammatory cytokine/chemokine production and dendritic cell (DC) maturation via the adaptor protein TICAM-1 (also called TRIF). Based on the different subcellular localization of cytosolic RNA receptors and TLR3, these receptors seem to play distinct roles in anti-viral immune responses.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
TLR3.7
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Matsumoto; M et al. Biochem Biophys Res Commun 2002; 293: 1364
References 2:
Oshiumi, H et al Nat Immunol 2003, 4: 161
References 3:
Matsumoto; M et al. J Immunol 2003; 171: 3154
References 4:
Burgener I et al. Vet Immunol Immunopathol 2008; 124
Toll-like receptors (TLR) are highly conserved throughout evolution and have been implicated in the innate defense to many pathogens. In Drosophila, toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defenses. In mammals, TLR identified as type I transmembrane signaling receptors with pattern recognition capabilities, have been implicated in the innate host defense to pathogens. TLR2 has been identified as a receptor that is central to the innate immune response to lipoproteins of Gram-negative bacteria, several whole Gram-positive bacteria, as well as a receptor for peptidoglycan and lipoteichoic acid and other bacterial cell membrane products. A functional interaction between TLR2 and TLR6 in the cellular response to various bacterial products has been discovered. The currently accepted paradigm regards TLR2 as an essential receptor for many eubacterial cell wall components, including lipoproteins and peptidoglycan. Bacterial species as diverse as mycobacteria, spirochetes, mycoplasma, Staphylococcus aureus, and Streptococcus pneumoniae have all been shown to mediate cellular activation via TLR2 (CD282). The monoclonal antibody TL2.3 is specific for human TLR2 (CD282). TL2.3 is useful for studies on the role of TLR2 as a pattern recognition receptor in microbial products induced cytokine production by TLR2 bearing cells such as human peripheral blood mononuclear cells. The monoclonal antibody TL23 is cross reactive with canine TLR2.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
TL2.3
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Flo; T et al. J Leukoc Biol 2001; 69: 474
References 2:
Schjetne, K et al J Immunol 2003, 171: 32
References 3:
Siedlar; M et al. J Immunol 2004; 173: 2736
References 4:
Tunheim G et al. Vaccine 2007; 25: 4723
References 5:
Burgener I et al. Vet Immunol Immunopathol 2008; 124: 184
The monoclonal antibody TL2.1 recognizes human Toll-like receptor 2 (TLR2, CD282). Toll-like receptors (TLR) are highly conserved throughout evolution and are involved in the innate defence to many pathogens. In Drosophila toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defences. In mammals, TLRs are identified as type I transmembrane signaling receptors with pattern recognition capabilities. They have been implicated in the innate host defence to pathogens. TLR2 is expressed on macrophages, smooth muscle, lung, spleen, thymus, brain and adipose tissue.<br /> TLR2 has been identified as a receptor that is central to the innate immune response to lipoproteins of Gram-negative bacteria, several whole Gram-positive bacteria, as well as a receptor for peptidoglycan and lipoteichoic acid and other bacterial cell membrane products. A functional interaction between TLR2 and TLR6 in the cellular response to various bacterial products has been discovered. TLR2 cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. It cooperates with TLR1 to mediate te innate immune response to bacterial lipoproteins or lipopeptides. It acts via MYD88 and TRAF6, leading to NF-κ-B activation, cytokine secretion and the inflammatory response. TLR2 also promotes apoptosis in response to lipoproteins.<br /> Bacterial species as diverse as mycobacteria, spirochetes, mycoplasma, S. aureus, B Burgdorferi, T pallidum, M fermentans and Streptococcus pneumoniae have all been shown to mediate cellular activation via TLR2.<br /> The monoclonal antibody TL2.1 is a TLR2 function blocking antibody that is useful for studies on the role of TLR2 as a pattern recognition receptor in microbial products induced cytokine production by TLR2 bearing cells such as human peripheral blood mononuclear cells
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
TL2.1
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Lien; E et al. J Biol Chem 1999; 274: 33419
References 2:
Flo, T et al J Leukoc Biol 2001, 69: 474
References 3:
Faure; E et al. J Immunol 2001; 166: 2018
References 4:
Droemann D et al. Histochem Cell Biol 2003; 119: 103
References 5:
Burgener I et al. Vet Immunol Immunopathol 2008; 124: 184
The monoclonal antibody T2.5 recognizes human Toll-like receptor 2 (TLR2). Toll-like receptors (TLR) are highly conserved throughout evolution and have been implicated in the innate defense to many pathogens. At present, ligands for several of the TLR's, such as TLR2-6,9, have been identified, confirming their role in first line defense against invading microorganism. In mammals, TLRs are identified as type I transmembrane signaling receptors with an extracellular portion containing leucine-rich repeats with pattern recognition capabilities. Pathogen recognition by TLRs provokes rapid activation of innate immunity by inducing proliferation of proinflammatory cytokines and upregulation of costimulatory molecules and eventually toinitiation of adaptive immunity. TLR2 has been identified as a receptor that is central to the innate immune response to lipoproteins of Gram-negative bacteria, several whole Gram-positive bacteria, as well as a receptor for peptidoglycan and lipoteichoic acid and other bacterial cell membrane products. It is suggested that TLR2 is able to recognize such a wide variety of PAMPs (pathogen-specific molecular patterns) by forming heterodimers with other TLRs like e.g. TLR6. TLR2 is essential for recognizing lipopeptides and lipoproteins from several microorganisms and also peptidoglycans derived from gram-positive bacteria. Bacterial species as diverse as mycobacteria, spirochetes, mycoplasma, Staphylococcus aureus, and Streptococcus pneumoniae have all been shown to mediate cellular activation via TLR2.
Monoclonal antibody mT2.7 reacts with mouse Toll-like receptor 2 (TLR2, CD282). Toll-like receptors (TLR) are highly conserved throughout evolution and have been implicated in the innate defense to many pathogens. In Drosophila toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defenses. In mammals, TLR identified as type I transmembrane signaling receptors with pattern recognition capabilities, have been implicated in the innate host defense to pathogens. TLR2 has been identified as a receptor that is central to the innate immune response to lipoproteins of Gram-negative bacteria, several whole Gram-positive bacteria, as well as a receptor for peptidoglycan and lipoteichoic acid and other bacterial cell membrane products. A functional interaction between TLR2 and TLR6 in the cellular response to various bacterial products has been discovered. The currently accepted paradigm regards TLR2 as an essential receptor for many eubacterial cell wall components, including lipoproteins and peptidoglycan. Bacterial species as diverse as mycobacteria, spirochetes, mycoplasma, Staphylococcus aureus, and Streptococcus pneumoniae have all been shown to mediate cellular activation via TLR2. The monoclonal antibody mT2.7 stained overexpressed, as well as endogenous cell surface- and intracellular TLR2. The antibody does not affect cell activation through TLR2.
The antibody binds to human TIMP-2. Reactivity in other species has not been determined, but the 11-mer peptide used for the immunization shows a 100% match with rat, mouse, and rabbit TIMP-2. The antibody was tested for cross-reactivity with TIMP-1 and did not cross-react.
This monoclonal antibody binds to human TIMP-1. Reactivity in other species has not been determined. The 11-mer peptide used for the immunization differs on at least 3 amino acids with the sequence of TIMP-1 of rat, mouse, and rabbit. Therefore it is not expected to be effective in these species as well. The antibody was tested for cross-reactivity with TIMP-2 and did not cross-react.
TIAR is an ubiquitously expressed RNA-binding protein, which regulates translational control, splicing, and other activities, including apoptosis. TIAR attenuates CDK1 activity, and is essential for the G2/M checkpoint. It accumulates in nuclear foci in late G2 phase and prophase in cells under replication stress. In steady state TIAR shuttles between the cytoplasm and the nucleus, probably as a part of nucleocytoplasmic transport of mRNA, but under stress conditions it accumulates mRNA molecules in granules and prevents their translation. Nucleolytic activity of TIAR against attacked target cells of cytotoxic lymphocytes has also been reported. Similarly, e.g. in permeabilized thymocytes TIAR triggers DNA fragmentation.SpecificityThe mouse monoclonal antibody W8B2B10 recognizes TNAP (tissue non-specific alkaline phosphatase), an ectoenzyme expressed mainly on embryonic stem cells, liver, bone, and kidney cells. This antibody is suitable for characterization of bone marrow-derived MSCs, iPSCs, and ESCs.Application detailsFlow cytometry: Intracellular staining; recommended dilution: 5 µg/ml
Antibody Isotype:
IgG2a kappa
Monosan Range:
MONOSAN
Clone:
6E3
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Originally, the 2G9 monoclonal antibody (mAb) was described as identifying a 15 kDa protein found in the cytoplasmic granules of cytotoxic T cells that might be part of a larger 40 kDa molecule, ubiquitously expressed, named p40-TIA-1 and often referred to as TIA-1 in the literature (1, 2). Now, however, there is evidence that the 2G9 mAb identifies a 17 kDa cytoplasmic granule membrane protein named GMP-17 that has no similarity with p40-TIA-1 (3). The GMP-17 antigen is a 165 amino acid protein with 4 transmembrane domains: but it is not a typical member of the fourtransmembrane superfamily. It is identical with previously identified cytotoxic granule proteins called NKG7 and GIG-1 for GCSF induced gene protein 1 , isolated from NK cells and granulocyte-colony-stimulatingfactor- treated mononuclear cells, respectively (4, 5). Pretreatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0, for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based detection is recommended. Control tissue Tonsil. Staining granular
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
2G9A10F5
Concentration:
n/a
Format:
Purified
Storage buffer:
Liquid purified Ascites, purified with Protein G Chromatography with 15mM Sodium Azide
Storage:
2-8°C
References 1:
Anderson, P. et al, 1990, J. Immunol., 2, 144, 574.
References 2:
Tian, Q., et al, 1991, Cell, 67, 629-639.
References 3:
Medley, et al, 1996, Proc. Natl. Acad. Sci. U S A, 93, 2, 685-9.
References 4:
Turman, M.A., et al, 1993, Hum. Immunol., 36, 1, 34-40.
References 5:
Shimane, et al, 1994, Biochem Biophys Res Commun., 199, 1, 26-32.
Thyroid Transcription Factor-1 (TTF-1) is a member of the homeodomain transcription factor family and plays a role in regulating genes expressed within the thyroid, lung and brain. These include thyroglobulin, thyroid peroxidase, Clara cell secretory protein and surfactant proteins. Human TTF-1 (38 kD) is a single polypeptide of 371 amino acids sharing 98% homology with the equivalent rat and mouse proteins. TTF-1 functions by binding to specific recognition sites in a manner that may be regulated by both the redox and phosphorylation status of the protein. In addition to its role as a tissue-specific transcriptional activator in adult organs, TTF-1 may also function in organogenesis. Gene targeting studies have shown TTF-1 to be essential for the proper development of the thyroid and lungs and abnormal expression may underline a number of congenital abnormalities.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
SPT24
Concentration:
Greater than or equal to 108 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Unal B et al. Turkish Journal of Pathology. 2014; 30(3): 201-205
References 2:
Klingen TA et al. Diagnostic Pathology. 2013; 8: 80-86
References 3:
Berghmans T et al. Lung Cancer. 2006; 52(2): 219-224
References 4:
Penman D et al. Journal of Clinical Pathology. 2006; 59:663-664.
References 5:
Comperat E et al. Modern Pathology. 2005; 18(10):1371-1376
Thyrotropin (hTSH) promotes the growth of the thyroid gland in the neck and stimulates it to produce more thyroid hormones. hTSH is composed of two subunits - alpha nad beta.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
TSH-51
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Thyrotropin (hTSH) promotes the growth of the thyroid gland in the neck and stimulates it to produce more thyroid hormones. hTSH is composed of two subunits - alpha nad beta.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
TSH-116
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Thyroid peroxidase gene expression is under the regulation of thyroid stimulating hormone. In normal thyroid, expression of thyroid peroxidase (TPO) described immunohistochemically is reported to produce a diffuse, fine, granular cytoplasmic stain in all follicular cells. Some studies have shown qualitative, as well as quantitative differences in thyroid peroxidase expression in thyroid cancer compared to normal tissue.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
AC25
Concentration:
Greater than or equal to 10.8 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Kimura S et al. Proceedings of the National Academy of Science USA 84: 5555-5559 (1987)
References 2:
De Micco C et al. Cancer 67(12): 30363041 (1991)
References 3:
Czarnocka B et al. Breast Journal Cancer 85(6): 875880 (2001)
References 4:
Tanaka T et al. Journal of Pathology 179: 8994 (1996)
Thyroglobulin (Tg) is the precursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3). Tg is a high molecular weight glycoprotein found in normal thyroid follicular cells. Thyroglobulin is useful for identifying thyroid carcinoma of papillary and follicular types and for identifying tumors of thyroid origin when working with adenocarcinoma of unknown primary
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN Ready To Use
Clone:
MRQ-41
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Sellitti DF and Suzuki K. Thyroid. 2014; 24:625-38
References 2:
Bellet D, et al. J Clin Endocrinol Metab 1983; 56:530-3
References 3:
Bejarano PA, et al. Appl Immunohistochem Mol Morphol. 2000; 8:189-94
Thyroglobulin (Tg) is the precursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3). Tg is a high molecular weight glycoprotein found in normal thyroid follicular cells. Thyroglobulin is useful for identifying thyroid carcinoma of papillary and follicular types and for identifying tumors of thyroid origin when working with adenocarcinoma of unknown primary.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN Ready To Use
Clone:
2H11+6E1
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Sellitti DF and Suzuki K. Thyroid. 2014; 24:625-38
References 2:
Bellet D, et al. J Clin Endocrinol Metab 1983; 56:530-3
References 3:
Bejarano PA, et al. Appl Immunohistochem Mol Morphol. 2000; 8:189-94
Thyroglobulin is a heavily glycosylated protein of 670kD composed of two identical subunits and is synthesized by the follicular epithelial cells of the thyroid. Thyroglobulin provides iodination sites for the formation of the thyroid hormones.
Antibody Isotype:
IgG2a
Monosan Range:
MONXtra
Clone:
1D4
Concentration:
n/a
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Male DK et al. Immunology. 54: 419427 (1985)
References 2:
Shepherd PS et al. European Journal of Nuclear Medicine. 10: 291295 (1985)
References 3:
Chan CTJ et al. Clinical and Experimental Immunology. 70: 516523 (1987)
Thrombomodulin is a transmembrane glycoprotein composed of 575 amino acids (molecular weight 75 kD) with natural anticoagulant properties. It is normally expressed by a restricted number of cells, such as endothelial and mesothelial cells. In addition, synovial lining and syncytiotrophoblasts of human placenta also express thrombomodulin. Antithrombomodulin has demonstrated positivity in benign vascular tumors such as hemangioma and most malignant vascular tumors (Kaposis sarcoma and epitheliod hemangioendothelioma). Hence, anti-thrombomodulin serves as a sensitive marker for lymphatic endothelial cells and their tumors. There has also been recent interest in the use of antithrombomodulin as an immunohiostochemical marker for mesothelial cells and malignant mesotheliomas. Anti-thrombomodulin is immunoexpressed in a variety of other tumors including urothelial cell carcinomas
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN
Clone:
1009
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Acebo E, et al. Histol. Histopath. 2001; 16:1031-6
References 2:
Appleton MA, et al. Histopathology. 1996; 29:153-7
References 3:
Attanoos RL, et al. Histopathology. 1996; 29:209-15
References 4:
Attanoos RL, et al. Histopathology. 2001; 39:584-8
Thrombomodulin is a transmembrane glycoprotein composed of 575 amino acids (molecular weight 75 kD) with natural anticoagulant properties. It is normally expressed by a restricted number of cells, such as endothelial and mesothelial cells. In addition, synovial lining and syncytiotrophoblasts of human placenta also express thrombomodulin. Antithrombomodulin has demonstrated positivity in benign vascular tumors such as hemangioma and most malignant vascular tumors (Kaposis sarcoma and epitheliod hemangioendothelioma). Hence, anti-thrombomodulin serves as a sensitive marker for lymphatic endothelial cells and their tumors. There has also been recent interest in the use of antithrombomodulin as an immunohiostochemical marker for mesothelial cells and malignant mesotheliomas. Anti-thrombomodulin is immunoexpressed in a variety of other tumors including urothelial cell carcinomas
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN Ready To Use
Clone:
1009
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Acebo E, et al. Histol. Histopath. 2001; 16:1031-6
References 2:
Appleton MA, et al. Histopathology. 1996; 29:153-7
References 3:
Attanoos RL, et al. Histopathology. 1996; 29:209-15
References 4:
Attanoos RL, et al. Histopathology. 2001; 39:584-8
Mouse anti Human TGF beta antibody, clone TB21 recognizes both human platelet-derived and recombinant TGF-beta1 in enzyme-linked immunosorbent assay (ELISA). Mouse anti Human TGF beta antibody, clone TB21 demonstrates neutralising activity against TGF-beta1 in cell proliferation assays. Mouse anti Human TGF beta antibody, clone TB21 has been demonstrated to react with dimeric (~25 kDa) or monomeric (~12.5 kDa) molecules of natural TGF-beta1 under non-reducing and reducing conditions respectively.
1E8-G6 reacts with TGF-alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. 1E8-G6 is completely blocked by the peptide used for immunization. TGFalpha (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. In sections both cytoplasma and cell membranes are stained
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
1E8-G6
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bebók, Zs, et al. Breast Cancer Res.Treatm. 29: 229-235 (1994)
TFG (TRK-fused gene protein) is a regulatory protein with not fully understood function. Its defects are associated with various carcinomas, such as e.g. melanoma, thyroid papillary carcinoma, or glioma. TFG structure (multiple protein interaction motifs) indicated it can be an adaptor protein. It has been demonstrated TFG interacts with proteins modulating the NFkappaB pathway (TANK and NEMO). TNG enhances the effect of TNFalpha, TANK, TRAF2 and TRAF6 in inducing NFkappaB activity.SpecificityThe antibody 2H11 recognizes thyroglobulin (TG), a 610 kDa extracellular secreted glycoprotein specific to the thyroid gland. TG is mainly expressed on thyroid follicular cells (99,1 %).Application detailsFlow cytometry: Recommended dilution: 1-5 ?g/ml.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
TFG-03
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Terminal deoxynucleotidyl transferase (TdT) is a DNA polymerase of 58 kD located in the cell nucleus which catalyzes the polymerization of deoxynucleotides at the 3' hydroxyl ends of oligo or polydeoxynucleotide initiators and functions without a template. TdT is reported to be expressed in primitive T and B lymphocytes of the normal thymus and bone marrow. The identification of TdT-positive cell populations in primary and secondary lymphoid organs during maturation of the immune system is one area of interest but it is the reported occurrence of high levels of enzyme activity in white blood cells and bone marrow in certain leukemias which is of particular interest.
Antibody Isotype:
IgG2a
Monosan Range:
MONXtra
Clone:
SEN28
Concentration:
Greater than or equal to 30 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Tai YC and Peh SC. FSingapore Medical Journal. 2003; 44(5):250-255
Tenascin C is an approximately 250 kDa extracellular matrix glycoprotein with important roles in the nervous system, as it promotes correct migration of growing axons during development and during neuronal regeneration. It is also involved in synaptic plasticity. Ligands of tenascin C are integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3, and alpha-V/beta-6. Similarly to neural cells, it also stimulates angiogenesis by promoting elongation and migration of endothelial cells.SpecificityThe mouse monoclonal antibody TFG-03 recognizes TFG, an approximately 50 kDa intracellular protein with regulatory functions.Application detailsImmunohistochemistry (paraffin sections): Immunohistochemical detection of tenascin is valuable for studies of tissue differentiation and tumour growth. The antibody T2H5 is excellent for staining of paraffin-embedded tissue sections.<br>Western blotting: Recommended dilution: 1-2 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
T2H5
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
EBS-O-166 specifically reacts with tenascin-C, an extracellular matrix glycoprotein of 210 kD. It recognizes those forms of tenascin that are produced by both normal and hyperproliferative (also neoplastic) tissues. Tenascin/hexabrachion/cytotactin is an extracellular matrix glycoprotein, widely expressed during embryogenesis. In adults, it is restricted to certain epithelial-stromal interfaces and increases markedly in hyperproliferative diseases and in stroma of many neoplasms, including gliomas, breast, squamous and lung carcinomas.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-O-166
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Verstraeten, AA et al., Br. J. Derm. 127(6), 571-574 (1992)
The antibody reacts with the 4th and 5th fibronectin-like repeats of human tenascin-C. It has been demonstrated that tenascin immunoreactivity in breast carcinoma cells could be indicative of metastasis and survival. Recent studies using retrospective material showed that the expression of tenascin in invasion border of early breast cancer significantly correlates with higher risk of distant metastasis. These studies have been continued now and the preliminary results clearly suggest that expression of tenascin in invasion border of early breast cancer is significantly associated with proliferative activity and higher risk of local recurrence. This result implicates a wide application for tenascin antibodies in the breast pathology.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
EB2
Concentration:
100 ug/ ml
Storage buffer:
PBS with 1% BSA & 0.1% sodium azide
Storage:
2-8°C
References 1:
Korhonen et al. J Histochem Cytochem 2000;48:1011-1020
References 2:
Karjalainen et al. Am J Resp Crit Care Med 2000;161:2086-2091
The antibody reacts with the fibrinogen-like knob-domain of tenascin protein. It has been demonstrated that tenascin immunoreactivity in breast carcinoma cells could be indicative of metastasis and survival. Recent studies using retrospective material showed that the expression of tenascin in invasion border of early breast cancer significantly correlates with higher risk of distant metastasis. These studies have been continued now and the preliminary results clearly suggest that expression of tenascin in invasion border of early breast cancer is significantly associated with proliferative activity and higher risk of local recurrence. This result implicates a wide application for tenascin antibodies in the breast pathology.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
DB7
Concentration:
100 ug/ ml
Storage buffer:
PBS with 1% BSA & 0.1% sodium azide
Storage:
2-8°C
References 1:
Pedrosa-Domellof et al. J Histochem Cytochem 2000;48:201-209
References 2:
Kaarteenaho-Wiik et al. J Histochem Cytochem 2000;48:1257-1268
TRIM (T cell receptor-interacting molecule), also known as TRAT1 (T cell receptor associated transmembrane adaptor 1) is a 30 kDa protein expressed by T cells as a cystein-linked homodimer. It associates with TCR-CD3-zeta-chain complex and becomes phosphorylated by Src-family kinases. TRIM is potentially involved in negative regulation of TCR-mediated signaling, but its role remains unclear. Flow cytometry: Recommended dilution: 1-4 µg/ml, intracellular staining. Western blotting: Non-reducing conditions.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
TRIM-04
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
The p53 family of proteins includes three members, p53, p63, and p73. The protein p63 is encoded by TP63 gene, which gives rise to protein isoforms with different properties and functions due to the presence (TAp63) or absence (deltaNp63) of an N-terminal transactivation domain. Immunohistochemistry of p63 has a clinical relevance for certain tumor types, but investigations have been hampered by a lack of well characterized antibodies that are specific for p63, do not cross-react with the related p53 and p73 proteins, and allow for discrimination between p63 isoforms TAp63 and deltaNp63 with opposite functional properties.SpecificityThe antibody T2H5 recognizes tenascin C, a large hexameric extracellular matrix glycoprotein.Application detailsWestern blotting: Recommended dilution: 1 ?g/ml
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
TAp63-4.1
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
EBS-T-059 recognizes an oncofetal antigen of 220kDa, identified as a tumor-associated glycoprotein (TAG72) with properties of a mucin and usually expressed by adenocarcinomas, but not by mesotheliomas and thus helps in the differential diagnosis of malignancies of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma. TAG72 is further used as serum marker for gastric cancer.
Tumor associated glycoprotein (TAG)-72 is a high molecular weight glycoprotein that is present on the surface of many neoplastic cells, including adenocarcinomas of the breast, colon, and lung. TAG-72 is found in lung adenocarcinoma and is absent in mesothelioma, making the TAG-72 antibody useful in distinguishing adenocarcinoma from mesothelioma.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN Ready To Use
Clone:
B72.3
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Thor, A, et al. Cancer Res 1986;46:3118
References 2:
Schlom J, et al. Tumormarker Oncology;1987;2:3
References 3:
Osteen KG et al. In J Gynecol Pathol. 1992 Jul;11(3):216-20
References 4:
Ordonez NG. Am J Surg Pathol. 1998 Oct;22(10):1203-14
References 5:
Chhieng DC et al. Hum Pathol. 2003 Oct;34(10):1016-21
The antibody is specific for Synaptophysin. The specificity was ascertained by immunoblotting and immunohistochemistry. The antibody predominantly reacts with a 38 kDa transmembrane glycoprotein from synaptic vesicles.
Synaptophysin is an integral membrane glycoprotein with a molecular weight of 38 kD. It is reported to occur in presynaptic vesicles of neurons in brain, spinal cord, retina, in similar vesicles of the adrenal medulla as well as in neuromuscular junctions. Synaptophysin may be involved in synaptic vesicle formation and exocytosis. Synaptophysin is reported to be expressed in a wide spectrum of neuroendocrine tumors including neuroblastomas, ganglioneuroblastomas, phaeochromocytomas, chromaffin and non-chromaffin paragangliomas. Synaptophysin is also reported to be expressed in neuroendocrine tumors of epithelial type including pituitary adenomas, islet cell tumors, medullary carcinomas of thyroid, parathyroid adenomas, carcinoids of the bronchopulmonary and gastrointestinal tracts, neuroendocrine carcinomas of the bronchopulmonary and gastrointestinal tract and neuronendocrine carcinomas of the skin.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
27G12
Concentration:
Greater than or equal to 42 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Takeda S et al. Neuropathology. 2003; 23(4):254261
Syk is a cytoplasmic protein tyrosine kinase that translocates to the plasma membrane upon B cell antigen receptor (BCR) or the high-affinity IgE receptor (FcepsilonRI) triggering, and phosphorylates downstream adaptor proteins, thereby providing docking sites for initiation of subsequent signaling pathways, such as calcium mobilization, cytoskeleton remodeling, or transcription of specific genes. Syk binds to the receptor assemblies through interactions of its pair of SH2 domains with ITAM motives of the receptor, which have been phosphorylated by Src-family kinases. These kinases also help to activate Syk by phosphorylation of its activation loop.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
SYK-01
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
SV40, Simian Virus 40 is a polyomavirus that is found in both monkeys and humans. Like other polyomaviruses, SV40 is a DNA virus that has the potential to cause tumors. SV40 is believed to suppress the transcriptional properties of tumor-suppressing p53 in humans through the SV40 large T-antigen and SV40 small T-antigen. It is generally assumed that large T-antigen is the major protein involved in neoplastic processes and the large T-antigen predominantly exerts its effect through deregulation of tumor suppressor p53, which is responsible for initiating regulated cell death (apoptosis), or cell cycle arrest when a cell is damaged. A mutated p53 gene may contribute to uncontrolled cellular proliferation, leading to a tumor.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN Ready To Use
Clone:
MRQ-4
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Gurney, E.G., et al. J Virl. 34:752-763 (1980)
References 2:
Huang, H., Reis,R. et al. Brain Pathol., 9:33-42 (1999)
References 3:
Arrington, A.S., et al. Molecular and Clinical Perspectives; 461-489 (2001)
STIM1 (stromal interacting molecule; also known as GOK) acts as a sensor of calcium depletion within the endoplasmic reticulum and transduces the signal to Orai1, the presumptive CRAC channel at the plasma membrane. Following decrease of luminal calcium concentration, STIM1 oligomerizes and induces Orai1 to enable entry of extracellular calcium into the cytoplasm. However, the precise mechanism of STIM1-Orai1 interaction has not been elucidated yet. Many questions also remain to be solved around STIM1 functional distribution. It turns out that STIM1 associates with growing ends of microtubules and is involved in endoplasmic reticulum tubule extension.SpecificityThe mouse monoclonal antibody TAp63-4.1 recognizes TAp63; target epitope LSDPMW. This antibody does not bind to deltaNp63 isoform of p63.Application detailsImmunocytochemistry: Methanol-aceton fixation; positive control: HeLa human cervix carcinoma cell line.<br>Immunohistochemistry (paraffin sections): Recommended dilution: 5 ?g/ml.<br>Western blotting: Recommended dilution: 1 ?g/ml; positive control: RBL rat basophilic leukemia cell line; both reducing and non-reducing conditions.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
CDN3H4
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of JAK kinases which then leads to tyrosine phosphorylation of proteins that have been designated Stats (signal transducers and activators of transcription). The first members of this family to be described include Stat1? p91, Stat1? p84 (a form of p91 that lacks 38 COOH-terminal amino acids) and Stat2 p113. Stat1 and Stat2 are induced by IFN-? and form a heterodimer which is part of the ISGF-3 transcription factor complex. Stat3, which becomes activated in response to epidermal growth factor (EGF) and interleukin-6 (IL-6), but not interferon-? (IFN-?) or Stat4, is an additional member of this family. It has been suggested that the phosphorylated forms of both Stat3 and Stat4 form homodimers as well as heterodimers with the other members of the Stat family, and that differential activation of different Stat proteins in response to different ligands should help to explain specificity in nuclear signaling from the cell surface. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Other members of the Stat family include Stat5, which has been shown to be activated by Prolactin and by IL-3, and Stat6 (also designated IL-4 Stat), which is involved in IL-4-activated signaling pathways. Pretreatment: Heat induced epitope retrieval in10 mM citrate buffer, pH6.0, for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Control tissue Urinary bladder. Staining Nuclear.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
D1
Concentration:
n/a
Format:
Concentrate
Storage buffer:
PBS Buffer, with less than 0.1% sodium azide and 0.1% gelatin
Storage:
2-8°C
References 1:
Zhong, Z., et al. 1994. Science 264: 95-98.
References 2:
Darnell, J.E., et al. 1994. Science 264: 1415-1421.
References 3:
Hou, J., et al. 1994. Science 265: 1701-1706.
References 4:
Yamamoto, K., et al. 1994. Mol. Cell. Biol. 14: 4342-4349.
STAT1 (signal transducer and activator of transcription 1) is a transcription factor that plays important roles in growth arrest, apoptosis promoting and tumour suppression. After ligation of cytokine receptors STAT1 becomes phosphorylated on Tyr701 by Janus kinase JAK1 or JAK2, dimerizes, translocates to nucleus and contacts DNA. STAT1-STAT2 heterodimers serve as more potent transcriptional inducers than STAT1 homodimers. STAT1 is also phosphorylated on Ser727 by MAPK pathway, independently of tyrosine phosphorylation. However, the both modifications are important for its maximal transcriptional activity. On the other hand, STAT1 phosphorylated on Ser727 is targeted for proteasomal degradation.SpecificityThe mouse monoclonal antibody CDN3H4 reacts with a cytoplasmic epitope of human and rodent STIM1, a 84 kDa essential and conserved regulator of store-operated Ca2+ channel function.Application detailsWestern blotting: Recommended dilution 1-2 ?g/ml, reducing conditions.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
SM2
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
STAT1 (signal transducer and activator of transcription 1) is a transcription factor that plays important roles in growth arrest, apoptosis promoting and tumour suppression. After ligation of cytokine receptors STAT1 becomes phosphorylated on Tyr701 by Janus kinase JAK1 or JAK2, dimerizes, translocates to nucleus and contacts DNA. STAT1-STAT2 heterodimers serve as more potent transcriptional inducers than STAT1 homodimers. STAT1 is also phosphorylated on Ser727 by MAPK pathway, independently of tyrosine phosphorylation. However, the both modifications are important for its maximal transcriptional activity. On the other hand, STAT1 phosphorylated on Ser727 is targeted for proteasomal degradation.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
SM1
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Analyte Specific Reagent. Analytical and performance characteristics are not established. The product is a lyophilized tissue culture supernatant containing sodium azide as a preservative. The user is required to reconstitute the contents of the vial with the correct volume of sterile distilled water as indicated on the vial label.
Antibody Isotype:
IgG2b
Monosan Range:
MONXtra
Clone:
RBC2/3D5
Concentration:
n/a
Storage buffer:
Lyophilized
Storage:
2-8°C
References 1:
Marafioti T et al. American Journal of Pathology. 162 (3): 861871 (2003
References 2:
Hess J et al. Molecular and Cellular Biology. 21 (5): 15311539 (2001)
References 3:
Re D et al. Cancer Research. 61 (5): 20802084 (2001)
References 4:
Luo Y and Roeder R G. Molecular and Cellular Biology. 15 (8): 41154124 (1995)
Spectrin is a cytoskeletal protein which is found in muscles, red blood cells and red cell precursors. Anti-Spectrin antibody is useful in the identification of blood dyscrasias and muscle disorders.
Antibody Isotype:
IgG2b-k
Monosan Range:
MONOSAN Ready To Use
Clone:
RBC2/3D5
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Sadahira, Y et al. J Clin Pathol. 1999 Dec; 52(12): 919-21
References 2:
Nehls, V et al. Am J Pathol. 1993 May; 142(5): 1565-73
References 3:
Muller M et al. J Vet Med A Physiol Pathol Clin Med. 2001 Feb;48(1):51-7
References 4:
Terada N et al. J Anat. 1997 Apr;190(Pt 3):397-404
SOX-11 which is a member of the SOX (SRY-related HMG-box) family is a transcription factor normally expressed in the developing human central nervous system and plays a role in embryonic cell determination. Studies show that SOX-11 can be used as a marker for mantle cell lymphoma (MCL). Mantle cell lymphoma (MCL) accounts for 5% to 10% of mature B-cell neoplasms and is an aggressive disease genetically characterized by overexpression of cyclin D1 (CCND1), an important regulator of the G1/S phase of the cell cycle, due to the specific translocation t(11;14)(q13;q32).
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MRQ-58
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
SOX-11 which is a member of the SOX (SRY-related HMG-box) family is a transcription factor normally expressed in the developing human central nervous system and plays a role in embryonic cell determination. Studies show that SOX-11 can be used as a marker for mantle cell lymphoma (MCL). Mantle cell lymphoma (MCL) accounts for 5% to 10% of mature B-cell neoplasms and is an aggressive disease genetically characterized by overexpression of cyclin D1 (CCND1), an important regulator of the G1/S phase of the cell cycle, due to the specific translocation t(11;14)(q13;q32).
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN Ready To Use
Clone:
MRQ-58
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Recognizes a protein of -55kDa, identified as SOX I 0. This MAb is highly specific and does not cross-react with other members of the SOX-family. SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA binding activity. SOX-10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. It is expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S I 00 protein. Commonly used melanoma markers, such as anti-HMB-45 and anti-Melan-A, are poorly expressed in desmoplastic melanomas while SOX-10 is moderately to strongly expressed in desmoplastic melanomas. SOX- 10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes, and myoepithelial cells of salivary, bronchial and mammary glands. SOX-I 0 expression is also observed in mast cells. Pretreatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0, for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based detection is recommended. Control tissue Melanomas, breast carcinomas, gliomas. Staining Nuclear
Antibody Isotype:
IgG2b, kappa
Monosan Range:
MONOSAN
Clone:
SOX10/1074
Concentration:
n/a
Format:
Concentrate
Storage buffer:
Bioreactor Concentrate with 0.05% Azide
Storage:
2-8°C
References 1:
Mohamed A, et al, Mol Morphol. 2013; 21(6):506-10.
The guanine nucleotide exchange factor Sos (son-of-sevenless) is a complex multidomain protein that activates the small GTPase Ras (H-Ras, K-Ras, N-Ras, but not functionally distinct R-Ras) in response to receptor tyrosine kinase stimulation. Nucleotide exchange activity of Sos is stimulated by allosteric Ras binding. By another (separable) guanine exchange factor domain domain Sos modulates activity of Rac/Rho GTPases. Sos thus integrates signals that affect both gene expression and cytoskeletal reorganization; the Sos-mediated Ras-activation and Rac activation differ in composition and stability of the formed complex.SpecificityThe antibody SM2 recognizes an epitope included within amino acids 8-23 of STAT1, a 91 kDa transcriptional factor involved in a variety of systems including antiviral responses and interferon alpha (IFN-alpha) and gamma (IFN-gamma) signal transduction.Application detailsWestern blotting: Recommended dilution: 1 ?g/ml; positive control: HeLa human cervix carcinoma cell line, reducing conditions.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
SOS-01
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
SOCS3 (suppressor of cytokine signaling 3), also known as CIS3 (cytokine-inducible SH2 protein 3) is a negative regulator of particular cytokine signaling pathways. SOCS3 is induced by a variety of cytokines and other stimuli, such as erythropoietin, leptin and lipopolysaccharides and inhibits tyrosinkinase activity of JAK kinases, or e.g. JNK phosphorylation. SOCS3 modulates cytokine-mediated and neoplastic-proliferative responses and is involved also in maintaining leukocytes in quiescent state until antigen stimulation.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
SO1
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Mouse anti Human SNAP-25 antibody, clone SP12 recognizes the human pre-synaptic protein, SNAP-25, also known as Synaptosomal-associated protein 25, Super protein (SUP) or Synaptosomal-associated 25 kDa protein. SNAP-25 is a 206 amino acid presynaptic protein of ~25 kDa containing two t-SNARE coiled-coil homology domains. Mouse anti human SNAP-25 antibody, clone SP12 will recognize SNAP-25 fusion protein from COS cells, but not the fusion protein from bacterial systems.Mouse anti Human SNAP-25 antibody, clone SP12 has been used to study the distribution of synaptic changes in the hippocampus of patients with medically refractory temporal lobe epilepsy. (Honer et al. 1994).
Smooth Muscle Myosin, heavy chain (SMMS-1) is a cytoplasmic structural protein that is a major component of the contractile apparatus of the smooth muscle cells. SMMS-1 is also a myoepitheliumassociated protein. Anti-SMMS-1 is a mouse monoclonal antibody to smooth muscle myosin, heavy chain that reacts with human visceral and vascular smooth muscle cells. The antibody also reacts with human myoepithelial cells. It is very helpful in distinguishing between benign sclerosing breast lesions and infiltrating carcinomas in difficult cases since it strongly stains the myoepithelial layer in the benign lesions while it is negative in the infiltrating carcinomas.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN
Clone:
SMMS-1
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Werling RW, et al. Am J Surg Pathol. 2003; 27:82-90
References 2:
Agoff SN, et al. Appl Immunohistochem Mol Morphol. 2001; 9:164-9
References 3:
Popnikolov NK, et al. Am J Clin Pathol. 2003; 120:161-7
References 4:
Lazard D, et al. Proc Natl Acad Sci USA. 1993; 90:999-1003
Smooth Muscle Myosin, heavy chain (SMMS-1) is a cytoplasmic structural protein that is a major component of the contractile apparatus of the smooth muscle cells. SMMS-1 is also a myoepitheliumassociated protein. Anti-SMMS-1 is a mouse monoclonal antibody to smooth muscle myosin, heavy chain that reacts with human visceral and vascular smooth muscle cells. The antibody also reacts with human myoepithelial cells. It is very helpful in distinguishing between benign sclerosing breast lesions and infiltrating carcinomas in difficult cases since it strongly stains the myoepithelial layer in the benign lesions while it is negative in the infiltrating carcinomas.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN Ready To Use
Clone:
SMMS-1
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Werling RW, et al. Am J Surg Pathol. 2003; 27:82-90
References 2:
Agoff SN, et al. Appl Immunohistochem Mol Morphol. 2001; 9:164-9
References 3:
Popnikolov NK, et al. Am J Clin Pathol. 2003; 120:161-7
References 4:
Lazard D, et al. Proc Natl Acad Sci USA. 1993; 90:999-1003
Smoothelin is a constituent of the smooth muscle cell (SMC) cytoskeleton. Antibodies directed to smoothelin are useful tools to monitor SMC differentiation. Smoothelin is exclusively expressed in fully differentiated (contractile) SMCs. RNA and protein analyses revealed a broad species distribution of this protein. Smoothelin has also been detected in smooth-muscle neoplasms. Cells with SMC-like characteristics, such as myofibroblasts and myoepithelial cells, as well as skeletal and cardiac muscle do not contain smoothelin. Confocal scanning laser microscopy of tissue sections and cells in culture show a filamentous organization of smoothelin colocalizing with actin stress fibers. In immunoblots two molecular weight isoforms are detected i.e. a 59 kDa isoform specific for visceral SMC (smoothelin A), and an isoform with a molecular weight of approximately 100 kDa in vascular SMC (smoothelin B). Human smoothelin is encoded by a single copy gene which is loCated on chromosome 22.
Smoothelin is a constituent of the smooth muscle cell cytoskeleton protein exclusively found in differentiated smooth muscle cells (SMC). Cells with SMC-like characteristics, such as myofibroblasts and myoepithelial cells, as well as skeletal and cardiac muscle do not contain smoothelin.1,2 To distinguish bladder muscularis mucosae (MM) from muscularis propria (MP) muscle bundles is crucial for accurate staging of bladder carcinoma. Strong smoothelin expression is nearly exclusively observed in muscularis propria. Therefore, the staining pattern of MP (strongly positive) and MM (negative or weakly positive) makes this technique an attractive diagnostic tool for the sometimes difficult task of staging bladder urothelial carcinoma, such as in transurethral resection specimens of urinary bladder tumors.3-5 Differentiating between smooth muscle tumors and other mesenchymal neoplasms of the GI tract can be challenging in small biopsies. Anti-smoothelin immunostaining can be helpful in differentiating benign (+) from malignant smooth muscle tumors (-), and other mimics(-).
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN Ready To Use
Clone:
R4A
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
van der Loop FT, et al. J Cell Biol. 1996; 134:401-11
References 2:
Maake C, et al. J Urol. 2006; 175:1152-7
References 3:
Paner GP, et al. Am J Surg Pathol. 2010; 34:792-9
References 4:
Council L, et al. Mod Pathol. 2009; 22:639-50
References 5:
Coco DP, et al. Am J Surg Pathol. 2009; 33:1795-801
NK cells make up approximately 10% - 25% of peripheral blood lymphocytes. In addition, NCAM is expressed on a variety of neural tissues and some tumors of neuro-endocrine origin, such as small cell lung cancer (SCLC). The CD56 antigen is not expressed on other immune cells. MOC-1 detects an isoform of the neural cell adhesion molecule (NCAM) expressed on natural killer (NK) cells of approximately 145 kDa
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MOC-1
Concentration:
50 ug/ml
Storage buffer:
0.01 M sodium phosphate, 0.15 M NaCl; pH 7.3, 0.2% BSA, 0.09% sodiumazide
Storage:
2-8°C
References 1:
De Leij, L., et al., 1985. Cancer Research 45: 2192-2200
This antibody is reactive with lung cancer associated antigens and has been studied and categorized in different clusters of reactivity patterns during the First International Workshop on Small Cell Lung Cancer Antigens held in London in April 1987. MOC-31 reacts with most epithelia, and, in lung cancer, all lung carcinomas. The membrane-associated proteins detected by MOC-31 appear to have an apparent molecular weight of 35-40 kD. Specificity: Epithelial specific Antigen/Ep-CAM - epithelial glycoprotein 2, EGP-2
Signaling from the ligand-activated membrane receptor serine/threonine kinases to nuclear targets is mediated by a set of evolutionarily conserved proteins known as SMADs. Upon ligand binding, the receptors of the TGF-? family phosphorylate SMAD proteins (SMAD1 and SMAD2). These proteins then move into the nucleus, where they activate transcription. To carry out this function, the receptor activated SMAD 1 and 2 require association with the product of deleted in pancreatic carcinoma, locus 4 (DPC4), also known as SMAD4. SMAD4/DPC4 is also implicated as a tumor suppressor, since it is inactivated in more than half of pancreatic carcinomas and to a lesser extent in a variety of other cancers. The lack of SMAD4 expression is present in approximately 80% of cases of pancreatic adenocarcinoma, but rarely in endometrial (0%), colorectal (0%), ovarian (3%), lung (0%), breast (2%) adenocarcinomas, and malignant melanoma (4%). SMAD4 is an important marker for confirming a diagnosis of pancreatic adenocarcinoma. Patients with pancreatic adenocarcinomas with SMAD4 protein expression had significantly longer survival than SMAD4 negative patients. Pretreatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0, or in 50 mM Tris buffer pH9.5, for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based detection is recommended. Control tissue Pacreatic adenocarcinoma. Staining Nuclear.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
B-8
Concentration:
n/a
Format:
Purified
Storage buffer:
Purified antibody fraction from mouse anti-serum with 0.2% BSA and 15mM sodium azide
The monoclonal antibody 31 recognizes secretory leukocyte proteinase inhibitor (SLPI). SLPI was identified as an alarm reactant and expression is induced by inflammatory factors like LPS, IL1β, TNF? and neutrophils elastase. SLPI is a relatively small basic antiprotease of 11.7 kDa and is a cationic non-glycosylated protein consisting of 107amino acids. SLPI has a high affinity for the neutrophil serine proteinases, elastase and cathepsin G. Orthologues of SLPI have been found in mice, rate, pigs and sheep. It consists of two highly similar WAP (âwhey acid proteinâ)/four-disulphide core domains. SLPI contain 16 cysteine residues which assemble into eight disulphide bridges (four in each WAP domain). SLPI is constitutively expressed at many mucosal surfaces and is produced by a variety of epithelial cells, including respiratory, intestinal and amniotic epithelia. Expression is also detected in mast cells, neutrophils and macrophages. Expression of SLPI gene is significantly increased by progesterone and by the pro-inflammatory cytokines TNF-? and IL1-β. Although SLPI has been shown to inhibit a spectrum of proteases (including HNE, cathepsin G, trypsin, chymotrypsin and chymase), its main action in this regard is likely to be the inhibition of elastase, as indicated by its low dissociation constant and favourable kinetics of inhibition for this enzyme. SLPI has been described in several body fluids like seminal fluid, bronchial fluids, cervical fluids and saliva. It has been found to be antibacterial, antifungal, anti-retroviral, and to have an important role in mucosal defence. SLPI might also facilitate tumor spread, contributing to wound healing, is elevated in sepsis and levels seem to correlate with oral candidiasis in HIV-1 positive patients. The reactivity of the antibody 31 with isolated domains of SLPI was evaluated using domains obtained by cleavage using partial acidic hydrolysis. Therefore, monoclonal antibody 31 recognizes also other SLPI cleavage products.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
31
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Wingens; M et al. J Invest Dermatol 1998; 111: 996
References 2:
Nakao, R et al Immunology 2003, 109: 271
References 3:
Aarbiou; J et al. Inflamm Res 2004; 53:230-238
References 4:
Tjabringa S et al. FEMS immunol and med microbial 2005; 45:151
SLP76 (SH2 domain-containing leukocyte protein of 76 kDa) is a cytosolic adaptor protein which translocates to the plasma mambrane and is involved in multiple signaling pathways in T cells, mast cells, neutrophils and platelets; B cells express its analog SLP65/BLNK (B cell linker protein). SLP76 is phosphorylated by Syk-family and Tec-family tyrosine kinases and couples them to the phosphorylation and activation of PLC-gamma. Via Gads or Grb2, SLP76 also associates with LAT adaptor by involvement of SLP76 proline-rich region. The SH2 domain of SLP76 has been identified as the region involved in binding the serine/threonine kinase HPK1. HPK1 may act as both a positive and a negative regulator by promoting the Jnk-mitogen activated protein kinase (MAPK) pathway and inhibiting the pathway leading to AP-1 activation.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
SLP-76/3
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Skp2 (S-phase Kinase-associated Protein 2) belongs to the family of F-box proteins that interact with the Cyclin A-Cdk2 complex. Skp2 is essential for the G1-S transition in both transformed cells and diploid fibroblasts. Biochemical and genetic experiments have demonstrated that Skp2 is required for the ubiquitination and consequent degradation of p27 in cultured mammalian cells and in vitro reconstitution assays. In normal tissues, Skp2 is expressed in tonsil and placenta.
SIT (SHP2-interacting transmembrane adaptor protein) is expressed exclusively in lymphoid organs and acts either as a positive or as a negative regulatory element in T cell activation and in T cell development. Binding to Grb2 plays a pivotal role in signal transduction. Hubener et al. (2001) determined that the SIT gene contains 5 exons and spans 1.8 kb of genomic DNA. The SIT promoter demonstrated strong transcriptional activity and potential binding sites for both ubiquitous and lymphoid-specific transcription factors.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
SIT-01
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
SHIP-1 (SH2 domain containing inositol phosphatase-1) is a 5´inositol phosphatase that regulates cell responses in lymphocytes and myeloid cells by hydrolyzing the second messenger PI(3,4,5) trisphosphate. SHIP-1 is recruited upon engagement of both inhibitory and activatory receptors, such as FcgammaRIIB, Fcgamma RIII, FcepsilonRI or cytokine and growth factor receptors, and supresses PI3K-dependent signaling, down-regulates cell migration and invasion of transformed cells and phagocytosis. SHIP-1 also serves as a scaffold for the recruitment of other proteins to the plasma membrane.SpecificityThe antibody SOS-01 reacts with human Sos, an ubiquitously expressed 150 kDa intracellular protein. The epitope sequence is highly conserved and reactivity with multiple species is expected.Application detailsFlow cytometry: Intracellular staining; recommended dilution: 2-5 ?g/ml; positive control: human blood leukocytes. <br>Western blotting: Positive control: RAMOS human cell line, reducing conditions.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
SHIP-01
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
The monoclonal antibody 13C4 recognizes the 1B subunit of Shiga-like toxin 1. Shiga-like toxins (SLTs), are also called Verotoxins. Enterohemorrhagic Escherichia coli (EHEC) strains which are primarily of serotypes 0157:H7, 026:H11 and O111:H8 have been incriminated as etiologic agents of hemorrhagic colitis and Hemolytic-uremic syndrome, a generalized disease characterized by acute renal failure, thrombocytopenia, and microangiopathic hemolytic anemia. There are several distinct E.coli SLTs. SLT-I and SLT-II are produced by EHEC. SLT-I and Shiga toxin share;99% deduced amino acid sequence homology, whereas SLT-I and SLT-II share about 60% deduced amino acid sequence homology. SLT-I and SLT-II are antigenically distinct. The protein structure of the toxin consists of two domains: the A polypeptide that inhibits protein synthesis by targeting ribosomes, and the B polypeptide pentamer that binds to the eukaryotic cell receptor globotriaosylceramide (Gb3) leading to receptor-mediated endocytosis.
The antibody reacts with several Staphylococcus strains using ELISA. It does not react with several members of the Enterobacteriaceae and not with S. Aureus. It is not tested for IHC-P. The epitope is not determined, but it might be a component of the cell wall. The antibody recognizes whole bacteria.
SCIMP (SLP adaptor and Csk interacting membrane protein), also known as Nvl, is a palmitoylated transmembrane adaptor protein expressed in professional antigen presenting cells, most prominently in the lymph nodes and spleen. It is associated with tetraspanin-enriched microdomains (together with MHC II). There is a close relationship between SCIMP and tyrosinkinase Lyn, which is constitutively bound to it by its SH3 domain. After MHC II-mediated stimulation in the immunological synapse SCIMP becomes phosphorylated at several tyrosine residues and provides docking sites for Grb2 and SLP65 or SLP76 adaptors transducing the signal downstream, as well as for the kinase Csk with modulatory roles.SpecificityThe antibody SHIP-01 reacts with SHIP-1, a phosphoinositide phosphatase largely confined to hematopoietic cells (intracellular antigen). Multiple forms of SHIP-1 have been reported with molecular weights of 110, 125, 130, 135 and 145 kDa.Application detailsFlow cytometry: Intracellular staining.<br>Western blotting: Recommended dilution: 1-2 ?g/ml.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
NVL-07
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, Schwann cells, epithelial and myoepithelial cells of the breast, salivary and sweat glands, as well as in glial cells.1,2,6 Neoplasms derived from these cells also express S-100 protein, albeit non-uniformly.1-4 A large number of well differentiated tumors of the salivary gland, adipose and cartilaginous tissue, 3 and Schwann cell-derived tumors express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S-100 protein.4,5 Despite the fact that S-100 protein is an ubiquitous substance, its demonstration is of great value in the identification of several neoplasms, particularly melanomas.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN Ready To Use
Clone:
4C4.9
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Nakajima T, et al. Ad J Surg Path. 1982; 6:715-727
References 2:
Kuhn HJ, et al. Am J Clin Path. 1983; 79:341-347
References 3:
Yaziji H, et al. Int J Surg Pathol. 2003; 11:11-5
References 4:
Patel P, et al. J Am Acad Dermatol. 2002; 46:264-70
References 5:
Morrison CD, et al. Semin Diagn Pathol. 2000; 17:204-15
The monoclonal antibody 27E10 recognizes an epitope specific for the S100A8/A9 heterocomplex that is not exposed on the individual subunits S100A8 (MRP8, calgranulin-A) or S100A9 (MRP14, calgranulin-B). The calcium-binding migration inhibitory factor-related proteins, MRP-8 (S100A8) and MRP-14 (S100A9) belong to the S100 protein family. The expression of these proteins is largely confined to the cytosol of neutrophils and monocytes. The complex formation of these proteins is a calcium-dependent process. The S100A8/A9 heterocomplex, also called MRP-8/MRP-14 complex or calprotectin, comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. The S100A8/A9 complex has antibacterial, antifungal, immunomodulating and antiproliferative effects. Besides this it is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity, like inflammatory bowel disease. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore calprotectin is also detectable in feces. Elevated levels of calprotectin have been observed in body fluids such as plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infection and inflammatory conditions.<br> The monoclonal antibody 27E10 can be used for early detection of inflammatory macrophages, for the characterization of tumorous tissues and the monitoring of peripheral blood cell cultures. The antibody 27E10 does not react with lymphocytes or platelets.
R-Ras2 / TC21 is the only member of R-Ras family of small GTPases that shows transforming activities similar to H-Ras, N-Ras, and K-Ras, and it is also structurally similar to them. R-Ras2 seems to play an important role in activating signal transduction pathways that control cell proliferation. Its mutations are associated with the growth of certain tumors, but also overexpression of the wild type form of R-Ras2 has been frequently detected in various carcinomas. Pseudogenes of R-Ras2 gene are found on chromosomes 1 and 2. Alternate splicing results in multiple transcript variants.SpecificityThe mouse monoclonal antibody NVL-07 recognizes intracellular part of human transmembrane adaptor SCIMP. This protein of 17 kDa predicted Mw migrates as a 22 kDa band on SDS PAGE.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml. Intracellular staining.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
EM-50
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Ribosomal protein SA (RPSA) is a multi-functional protein, that is an important component of 40S ribosomal subunit, and binds to lamin. Higher expression of RPSA is characteristic for many carcinomas, and correlates with their invasivity and metastatic potential. It has also been described, that RPSA interacts with amyloid beta peptide during Alzheimer´s disease.SpecificityThe mouse monoclonal antibody EM-50 recognizes R-Ras2 / TC21 protein (intracellular antigen) and does not react with R-Ras1, H-Ras, K-Ras, and N-Ras.Application detailsFlow cytometry: Recommended dilution: 8-12 ?g/ml. Intracellular staining.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
RP-01
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
RLTPR / CARMIL2 (RGD motif, leucine rich repeats, tropomodulin domain and proline-rich containing; capping protein regulator and myosin 1 linker 2), also known as LRRC16C, is a cytosolic protein, which with high affinity binds CAPZA2 (capping protein muscle actin Z-line alpha 2) and decreases CAPZA2 affinity for actin barbed ends. RLTPR / CARMIL2 increases the rate of actin filament elongation from seeds in the presence of CAPZA2, however, seems unable to nucleate filaments. Its interaction with CAPZA2 is essential for lamellipodial protrusion and cell translocation. RLTPR / CARMIL2 is crutial for T cell costimulation via CD28 and this property seems to be independent on its actin-uncapping function. The lack of functional RLTPR / CARMIL2 molecules impeded the differentiation toward Th1 and Th17 fates of both human and murine CD4+ T cells and leads to combined immunodeficiency. Expression of RLTPR / CARMIL2 was also detected in human and murine B cells, but it seems not to be involved in BCR-mediated signaling.SpecificityThe mouse monoclonal antibody RP-01 recognizes ribosomal protein SA (RPSA), which is important for formation and stability of 40S ribosomal subunit, and is overexpressed in many carcinomas.Application detailsFlow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
EM-53
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
58-15 Recognizes riboncleoproteins (RNP), found predominantly in nuclear ribonucleoprotein (nRNP) particles, one of the main components of nucleoli. It identifies cells active in the cell cycle and hence can be used to measure the mitotic activity of cell populations. Since the antibody can be used in paraffin embedded tissue sections, it can identify actively cycling cells within routinely fixed tissue specimens. 58-15 Can be considered a pan nRNP antibody. Pan nRNP antibodies provide detection for a range of RNP proteins.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
58-15
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Clevenger, C.V. et al. J Histochem Cytochem 32: 757-765 (1984)
References 2:
Clevenger, C.V. et al. Cytometry 6: 208-214 (1985)
References 3:
Maryam A and Nigel WF, J Virol. 75: 12070-12080, (2001)
Retinoblastoma (Rb) is a rare tumor of the retina associated with mutations of chromosome 13. The nuclear phosphoprotein encoded by the Rb tumor suppressor gene is present in many cells and may indirectly regulate cell growth by activating the transcription factor ATF-2. Activation of ATF-2 initiates expression of TGF-beta2, which in turn inhibits transcription of genes affecting cell growth. Bilateral mutation of the Rb gene may potentially play a role in the development of a number of malignant tumors.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
13A10
Concentration:
n/a
Storage buffer:
Tissue culture supernatant with 15mM sodium azide
Storage:
2-8°C
References 1:
Jares P et al. Journal of Pathology. 182: 160-166 (1997)
References 2:
Karpeh MS et al. British Journal of Cancer. 72: 986-991 (1995)
References 3:
Stefanini M et al. Nature. 216: 173-174 (1967)
References 4:
Bartek J et al. Oncogene. 7: 101-108 (1992)
References 5:
Sanders BM et al. British Journal of Cancer. 60: 358-365 (1989)
The antibody stains over 95% of primary renal cell carcinomas and 60% of metastases of renal cell carcinoma and glomerular visceral epithelium and proximal tubules in normal kidney (cytoplasmic). It does not stain epithelial cells of non-renal malignancies.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
RC38
Concentration:
15 ug/ml
Storage buffer:
Culture medium with 0.01M Sodium Azide
Storage:
2-8°C
References 1:
Oosterwijk E et al. (1986). Am J Pathol 123, 301-309
Anti-renal cell carcinoma (RCC) recognizes a 200 kD glycoprotein localized in the brush border of the proximal renal tubule. This antibody immunoreacts with approximately most primary renal cell carcinomas and can aid in the diagnosis when renal cell carcinoma enters the differential diagnosis. Other tumors that may react with this antibody are parathyroid adenoma, an occasional breast carcinoma. Nephroblastoma, oncocytoma, mesoblastic nephroma, transitional cell carcinoma, and angiomyolipoma are not labeled with this antibody.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN Ready To Use
Clone:
PN-15
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Dabbs, D. 4th Edition. Elsevier Saunders. 2014; p234
References 2:
Bakshi N, et al. Appl Immunohistochem Mol Morphol. 2007; 15:310-5
References 3:
McGregor DK, et al. Am J Surg Pathol. 2001; 25:1485-92
References 4:
Avery, AK et al. Am J Surg Pathol 24(2): 203-210, 2000
G4E4 recognizes an epitope within the 74-182 C-terminal sequence (11kD peptide fragment) of human serum Cellular Retinol Binding Protein 1 (CRBP 1), a single-chain glycoprotein belonging to the superfamily of hydrophobic molecule transporter proteins, which is responsible for transport of retinol (vitamin A1) from the liver to peripheral target tissues, like the eye, where it mediates the cellular uptake. CRBP 1 is synthesized by hepatic parenchymal cells where it becomes bound to its ligand retinol and is then released into the circulation, where it binds further to the protein transthyretin, to form a transporting complex, which is big enough not to be lost by filtration through the kidney glomeruli. It is detected in nearly all tissues with higher expression in adult ovary, pancreas, pituitary gland, adrenal gland, and fetal liver.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
G4E4
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Reddy B. et al. Biochem. Int. 21: 367-376 (1990)
References 2:
Reddy B. et al. Molec. Immunol. 29: 511-516 (1992)
References 3:
Reddy B. et al. Molec. Immunol. 30: 1355-1360 (1993)
This monoclonal antibody recognizes a cell surface structure of about 80 kD expressed by rat tumour cells of epithelial origin. MG1 is a rat strain-independent markers for tumour cells of epithelial origin, such as colon, breast, or lung cancer, etc. When injected in colon tumour-bearing rats, MG1 localizes to tumour cells (Hagenaars et al., 2001).
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
MG1
Concentration:
n/a
Storage buffer:
Culture supernatant with 0.05% sodium azide
Storage:
2-8°C
References 1:
Hagenaars M et al. Clin Exp Metastasis 2000;18:189-196
References 2:
Hagenaars M et al. Clin Exp Metastasis 2001;18:281-289
v CC52 is a rat strain-independent marker for tumour cells of epithelial origin, such as colon, breast, or lung cancer, etc. When injected in colon tumour-bearing rats, CC52 localized to tumour cells (Hagenaars et al., 2001). The monoclonal antibody binds to a dimer of two proteins, 120 kD and 130 kD, expressed by rat tumour cells of epithelial origin.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
CC52
Concentration:
n/a
Storage buffer:
Culture supernatant with 0.05% sodium azide
Storage:
2-8°C
References 1:
Hagenaars M et al. Clin Exp Metastasis 2000;18:189-196
References 2:
Hagenaars M et al. Clin Exp Metastasis 2001;18:281-289
This bispecific antibody was generated by fusion of the R73-producinghybridoma with the CC52-producing hybridoma (Beun et al., 1993).Quadroma clones producing functional bispecific antibodies were selectedby testing the ability of the quadroma products to induce T cells to lyse theappropriate tumor cells.
The R73-2b monoclonal antibody binds to an epitope of the constant region of the rat ?ß-T cell receptor and is able to activate rat T cells (Beun et al., 1993). The reactivity of R73-2b monoclonal antibody is rat strain-independent. R73-coated culture flasks induce rat T cell proliferation (Beun et al., 1993). This monoclonal antibody is an IgG2b isotype switch variant (Beun et al., 1993) of the well-known R73 IgG1 monoclonal antibody that is specific for the rat ?ß-T cell receptor (Hünig et al. 1989).
Monoclonal antibody 3D12 reacts with rat class B scavenger receptor type I (SR-BI). Scavenger receptors have been studied primarily for their ability to bind and internalize modified lipoproteins. They have been found in the development of atherosclerosis and other macrophage-associated functions. Scavenger receptors also function as pattern recognition receptors for a wide variety of pathogens. This finding indicates a potential role in host defense. SR-BI belongs together with CD36 to the class B scavenger receptor family. SR-BI is a multiligand membrane protein existing in various organs such as the liver and various cell types such as endothelial cells, macrophages, brain cells, Leydig cells and Sertoli cells. SR-BI has been found as a receptor for phospholipids, free and (lipo)protein-bound ApoE, lipid-bound ApoA-I, HDL, hypochlorite-modified LDL and more. In liver, the PDZK-1 (and possible other PDZ domains) of SR-BI has been found to be essential for cell surface expression and, hence, reverse cholesterol transport. In the brain, the presence of SR-BI seems to be involved in the uptake of oxidatively modified lipoproteins and beta-amyloid protein complexed with ApoE, suggesting SR-BI to be an important tool for studies on neurodegenerative disorders. In the testis, SR-BI is expressed in two somatic cell types: Leydig cells and Sertoli cells. SR-BI functions at least partly as a phosphatidyl serine receptor (PSR), enabling Sertoli cells to recognize and phagocytose apoptotic spermatogenic cells at all stages of differentiation. Monoclonal antibody 3D12 blocks the biological activity of rat SR-BI. For example, it inhibits the ability of SR-BI to mediate the corporation of lipids of HDL by SR-BI expressing cells.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
3D12
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Nakagawa; A et al. Develop Growth Differ 2004; 46: 283
ANK61 reactivity is rat strain-independent. The antigen recognized by ANK61 is highly expressed on freshly isolated and cultured rat NK cells. The antibodies also bind to rat ?ß-TCR T cells and at a low level to rat B cells. Binding to other cell types is unknown. Triggering of the antigen by ANK61 antibodies activates the lytic machinery of rat NK cells, but not of T cells.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
ANK61
Concentration:
n/a
Storage buffer:
Culture supernatant with 0.05% sodium azide
Storage:
2-8°C
References 1:
Giezeman-Smits KM et al. Immunobiol 1997;197:429-443
The monoclonal antibody recognizes rat CD8 antigen (MW 34 kDa and 39 kDa) and is reactive with all common inbred strains. 15-11C5 is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from Balb/c mice immunized with WAG/Rij spleen cells.
The monoclonal antibody recognizes rat CD4 antigen (MW 52 kDa) and is reactive with all common inbred strains. 15-8A2 is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from Balb/c mice immunized with WAG/Rij spleen cells.
This is a rat strain-independent antibody to CD45. This monoclonal antibody recognizes a common epitope of rat CD45, present on all rat leucocytes. The ANK74 monoclonal antibody was generated by immunizing mice with IL-2-activated cultured NK cells of Wag rats.
Dipeptidyl peptidase IV (DPP IV) is widely distributed in a number of mammalian tissues and is suggested to play an important role in various kinds of biological processes. DPP IV (CD26) is a serine-type protease that removes the amino-terminal dipeptide from peptide substrate provided that the penultimate amino acid residue is proline or alanine. DPP IV plays an important role in the reclamation of peptide nitrogen from larger peptides. The monoclonal antibody 5E8 reacts with DPP IV present on the apical surface of epithelial cells in the pancreas, small intestine, colon, and bile duct. Furthermore antibody 5E8 reacts with DPP IV on the laminar portions of the proximal renal tubule cells, and, weakly, on the glomeruli.
The asialoglycoprotein (ASGP) receptor is a transmembrane hepatocellular surface carbohydrate binding glycoproteins lacking terminal sialic acid residues (asialoglycoproteins). Characterization of the ASGP receptor- revealed its functional role in the binding, internalization and transport of a wide range of glycoproteins, which have exposed galactose or N-acetylgalactosamine residues, via the process of receptor-mediated endocytosis (RME). The ASGP receptor can bind a variety of important plasma proteins including transport proteins (i.e. transferrin), enzymes such as alkaline phosphatase, immunoglobulins including IgA, apoptotic hepatocytes, fibronectin and platelets. Additionally, the expression of the ASGP receptor has been clinically correlated to the level of hepatic function that is lost during liver diseases related to cancer, viral hepatitis, and cirrhosis. The ASGP receptor consists of major and minor subunits, which in the rat were identified as rat hepatic lectin (RHL) 1 and RHL 2/3, with molecular weights of respectively 42, 49 and 54 kDa. The selective binding (calcium and pH depended) and uptake of terminal galactosyl bearing proteins requires the formation of hetero-oligomers between these major and minor forms. The total ASGP receptor population consisted of two functionally distinct receptor populations, designated State 1 and State 2, which were involved in the endocytosis and intracellular processing of ligands by different pathways. The monoclonal antibody 8D7 recognizes a subunit-specific epitope on RHL-1 of rat ASGPR. The monoclonal antibody 8D7 is cross reactive with human ASGPR.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
8D7
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Mizuno; M et al. Gastroenterol Japan 1986; 21: 238
The antigen recognized by ANK44 is highly expressed on rat NK cells after IL-2-activation. The antigen is not expressed by unstimulated NK cells. ANK44 also binds to rat ??-TCR T cells. It does not bind to ?ß-TCR T cells or to B cells. The ANK44 monoclonal antibody is rat strain-independent.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
ANK44
Concentration:
n/a
Storage buffer:
Culture supernatant with 0.05% sodium azide
Storage:
2-8°C
References 1:
Giezeman-Smits KM et al. J Leukoc Biol 1998;63:209-215
RAb-50 reacts with SAD-Vnukovo and Pitman-Moore strains of rabies virus. It shows conformation dependent specificity for the viral envelope glycoprotein. In immunofluorescence test, SAD-Vnukovo strain is recognized by the antibody, while in Western blot, both strains are recognized by the antibody. In ELISA only SAD-Vnukovo strain is recognized. RAb-50 can neutralize the CVS strain of rabies virus.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
RAB-50
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Macikova, I. et al, Acta virol. 36: 541-550 (1992)
References 2:
Sajjanar B, et al, Neuropeptides. 57: 59-64 (2016)
References 3:
Chen Li, et al, Acta Pharmacol Sin. 32(3): 329337 (2011)
Protein tyrosine phosphatases (PTPs) are the enzymes that are instrumental in determining the spatial and temporal balance between the tyrosine phosphorylated and non-phosphorylated targets, and thus coordinately regulate these cellular responses to extracellular cues. The Ptprr gene gives rise to 4 different neuronal phosphatases which differ in length of their Nterminal part and subcellular localization. PTPBR7 (72 kDa) is receptor-like, PTP-SL (60 kDa) is membrane associated and PTPPBS? (42 and 37 kDa) is a cytosolic phoaphatase. This antibody is directed to the common part of these proteins.
Antibody Isotype:
IgG2
Monosan Range:
MONOSAN
Clone:
6A6
Concentration:
n/a
Storage buffer:
lyophilized in a 10 mM ammonium bicarbonate buffer. Each vial contains 2 mg BSA
PTEN gene is a tumor suppressor gene that maps to chromosome 10q23. PTEN, a novel tumor suppressor, functions as a regulator of both cell cycle progression and apoptosis ). Potentially, mutation and deletion of PTEN gene may result in a new signal transduction pathway related to human malignant tumors. Studies have demonstrated a reduction of PTEN expression in advanced breast cancers. Positive control tissue Breast, Renal Cell and Prostate carcinomas
Glutamate carboxypeptidase II (GCPII), also known as N-acetyl-alpha-linked acidic dipeptidase I (NAALADase I), folate hydrolase (FOLH1), and prostate-specific membrane antigen (PSMA), is an approximately 95-110 kDa type II transmembrane glycoprotein expressed in various tissues. In nervous system GCPII cleaves abundant N-acetylaspartylglutamate, which is released from neurons in a calcium-dependent manner, to N-acetylaspartate and glutamate. As immoderate glutamate concentration is neurotoxic, GCPII contributes to pathological conditions regarding e.g. Alzheimer´s disease, Huntington´s disease, epilepsy, schizophrenia, stroke or neuropathic pain and appears to be an interesting therapeutic target. In jejunum GCPII hydrolyzes pteroylpoly-gamma-glutamate to folate and glutamate, enabling folate to be absorbed by gastrointestinal tract. GCPII, which is present in a number of tissues at low levels, is overexpressed in neovasculature of most solid tumours and is a target enzyme for diagnosis and treatment of prostate cancer. Normal human prostate express more mRNA coding for a cytosolic GCPII form truncated at the N-terminus (PSM´) than mRNA for membrane-bound GCPII, and this ratio is reversed upon malignant transformation.SpecificityThe mouse monoclonal antibody EM-53 recognizes RLTPR / CARMIL2, an intracellular protein playing a role in actin filament elongation.Application detailsWestern blotting: Recommended dilution: 1 ?g/ml; positive control: LNCaP cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold lysis buffer (1% NP-40). Incubate 30 min on ice. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer. Both reducing and non-reducing conditions.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
GCP-04
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Prostate-specific antigen (PSA) is a protein produced by normal prostate cells. This enzyme participates in the dissolution of the seminal fluid coagulum and plays an important role in fertility. The highest amounts of PSA are found in the seminal fluid; some PSA escapes the prostate and can be found in the serum. This serum component has been used to track the response to therapy in men with prostate cancer.SpecificityThe mouse monoclonal antibody GCP-04 recognizes amino acids 100-104 of extracellular domain of denaturated glutamate carboxypeptidase II (PSMA, NAALADase, FOLH1), an approximately 95-110 kDa transmembrane glycoprotein.Application detailsWestern blotting: Positive material: seminal plasma, reducing conditions, recommended antibody dilution: 2-5 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
A67-B/E3
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
The antibody reacts with prostatic acid phosphatase in the glandular epithelium of normal and hyperplastic prostate, and adenocarcinoma of the prostate. Anti-PSAP is useful in identifying prostatic origin of tumors in the metastatic setting. PSAP complements other immunohistochemical markers in the correct clinical context.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN Ready To Use
Clone:
PASE/4LJ
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Ansari, MA, et al. Am J Clin Path 1981;76:94-98
References 2:
Kimura, N, et al. Virchows Arch A 1986;4:247-251
References 3:
Kidwai N et al. Breast Cancer Res. 2004;6(1):R18-23
References 4:
Genega EM et al. Mod Pathol. 2000 Nov;13(11):1186-91
References 5:
Gatalica Z et al. Appl Immunohistochem Mol Morphol. 2000 Jun;8(2):158-61
Prostate-Specific Antigen (PSA) is a 33 kDa protein primarily produced by the prostatic epithelium and the epithelial lining of the periurethral glands. PSA is strongly expressed in both normal and neoplastic prostatic tissue. Although PSA can be considered prostate-specific, PSA and/or PSA gene expression has been detected at low levels in some extra-prostatic tissues such as normal breast tissue, breast tumors, endometrium, adrenal neoplasms and renal cell carcinomas. Anti-PSA is most useful in determining the prostatic origin of carcinomas in non-prostate tissues (metastatic disease) using IHC techniques. This product is best used in conjunction with a panel of antibodies as, up to 27% of prostate carcinoma cases (predominantly poorly differentiated carcinomas) can be negative for this marker.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN Ready To Use
Clone:
ER-PR8
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Polascik TJ, et al. J Urol. 1999; 162:293
References 2:
Stenman UH, et al. Semin Cancer Biol. 1999; 9:83-93
References 3:
Alanen KA, et al. Path Res Pract. 1996; 192:233-237
PRR7/TRAP3 (proline-rich 7, transmembrane adaptor protein 3) is a 28 kDa transmembrane adaptor protein ubiquitously expressed at low level (most in brain). Its amino acid sequence is extremely conserved among mammalian and other species. PRR7/TRAP3 contains potential palmitoylation motif and is found in lipid rafts. It is a part of the complex postsynaptic density fraction in neurons and associates with PSD-95, NMDA receptor and probably other proteins. The intracellular domain of PRR7/TRAP3 contains several tyrosines, a proline-rich sequence, and a C-terminal PDZ-binding motif. So far nothing is known about function of this protein. It may be involved in regulation of some receptor signaling and in formation of neurologic and immunologic synapse.SpecificityThe mouse monoclonal antibody A67-B/E3 reacts with prostate-specific antigen (PSA), a protein produced by normal prostate cells. The highest amounts of PSA are found in the seminal fluid. This antibody recognizes both free and complexed PSA.Application detailsImmunocytochemistry: Recommended dilution: 10 ?g/ml; cell culture fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton-X100. <br>Western blotting: Recommended dilution: 1 ?g/ml; positive control: murine brain lysate (red. Laemmli buffer).
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
TRAP3/10
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
PN-15 reacts with a lectin receptor like glycoprotein of 200 kDa (gp200), present in proximal renal tubules and on urothelium. The antigen is carbohydrate in nature. Other normal tissues that display the antigen include breast, parathyroid glands, thymus and epididymis. Among renal carcinomas 93% of primary and 84% of metastatic carcinomas are positive. Bladder cancers are also largely positive. Other tumor types include breast cancer, teratocarcinomas and parathyroid adenomas. The antigen, also called DEC-205, was assigned to CD205 at CD workshop VII. In the immune system it can facilitate tolerance to self-antigens through uptake of apoptosis derived material by dendritic cells, which in turn present fragments through MHC II and MHC I, either inducing or repressing immune responses, depending on the nature of concomitant signals.
Antibody Isotype:
IgG2b-kappa
Monosan Range:
MONOSAN
Clone:
PN-15
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Yoshida, S.O. et al, Cancer Res 49: 1802-1809 (1989)
References 2:
Li, G, et al, Anticancer Res. 20(4): 2773-8 (2000)
References 3:
Batchelder C.A. et al, Anat Rec (Hoboken) 297(8): 1392-1406 (2014)
References 4:
Cykowski M.D. et al, Ultrastruct Pathol 39(1): 69-77 (2015)
The antibody reacts with bacteria in suspension and is therefore useful for detection of Proteus spp. in bacterial suspensions. Various systems can be used with this antibody to detect the presence of Proteus spp.. The antibody reacts with approximately 50% of the Proteus spp. isolates tested.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
31-17
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide and 0.1% BSA
Storage:
2-8°C
References 1:
Widjojoatmodjo, M.N., et al., 1991, Eur. J. Clin. Microbio. Infect. Dis. 10, 935-38
Lck is a Src-family tyrosine kinase, which is essential for signaling through the T-cell receptor (TCR) complex. Upon TCR triggering, Lck phosphorylates the ITAM motives in its zeta subunits, establishing binding sites for the SH2 domains of the tyrosine kinase ZAP70, which is also phosphorylated by Lck and thereby activated to generate subsequent signaling platforms by phosphorylation of adaptor LAT. Whereas the majority of Lck is localized to the plasma membrane, there is also a significant fraction associated with the Golgi apparatus, which may contribute to Raf activation under conditions of weak stimulation through the TCR. Lck is also involved in the regulation of apoptosis induced by various stimuli, but not by the death receptors.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
LCK-01
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Protein gene product (PGP) 9.5 is a neuron-specific protein, structurally and immunologically distinct from neuron specific enolase. The protein which has a molecular weight of 27 kD was first identified by high resolution two dimensional PAGE. PGP9.5 expression has been reported in neurons and nerve fibers at all levels of the central and peripheral nervous system, in many neuroendocrine cells, in segments of the renal tubules, in spermatogonia and Leydig cells of the testis, in ova and in some cells of both the pregnant and non-pregnant corpus luteum. PGP9.5 is a member of the ubiquitin C-terminal hydroxylase family and is also concentrated within inclusion bodies suggesting that such structures may be metabolically active regions of the cells.
Antibody Isotype:
IgG2b
Monosan Range:
MONXtra
Clone:
10A1
Concentration:
Greater than or equal to 35 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Beresford L et al. Immunology. 2004; 111(1):118125
The human intracellular serpin proteinase inhibitor 9 (PI9) is the only human protein able to inhibit the activity of the serine protease granzyme B. Granzyme B is expressed by cytotoxic lymphocytes and induces rapid target cell apoptosis. PI9-17 Mab was selected after immunization with full length recombinant PI9 produced in Escherichia coli. No cross reactivity with other, homologous serpins (PI6, PI8 and PAI-2) was observed.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
PI9-17
Concentration:
250 ug/ml
Storage buffer:
serumfree culture supernatant with 0.7% BSA and 0.1% sodium azide
Storage:
2-8°C
References 1:
Bird CH et al. Mol.Cell.Biol 1998; 18: 6387
References 2:
Bladergroen BA et al. J of Immunol 2001; 166: 3218-325
Prostatic acid phosphatase (PAP) is an isoenzyme of acid phosphatase found in large amounts in the prostate and seminal fluid. The precise function of PAP is unknown, but it may act as a hydrolase to split phosphoryl choline in semen and also function as a transferase. Elevated serum levels of the enzyme are reported in metastatic prostatic carcinoma.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
PASE/4LJ
Concentration:
n/a
Storage buffer:
Tissue culture supernatant with 15mM sodium azide
Storage:
2-8°C
References 1:
Haines AMR et al. British Journal of Cancer. 60: 887892 (1989)
References 2:
Haines AMR et al. Biochemical Society Transactions. 15: 11791180 (1987)
The presence of prostate specific antigen is highly specific for demonstration of the prostatic origin of a malignancy. Mab ER-PR8 has been shown to be a valuable reagent for the identification of primary and metastatic prostatic carci-noma. The antibody reacts with a 34kD protein in benign and malignant prostatic epithelium. Positive control: Prostatic benign hyperplasia.
Prostate specific antigen (PSA) is a 34 kD protein belonging to the kallikrein family of serine proteases and was originally isolated and purified from human seminal plasma. It was found to be immunologically identical and biologically similar to a protein isolated from the prostate gland. PSA is distinct from prostatic acid phosphatase. Low levels of expression of PSA have been reported in non-prostatic tissues and tumors such as breast carcinomas.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
35H9
Concentration:
Greater than or equal to 43 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Watt KWK et al. Proceedings of the National Academy of Sciences USA. 1986; 83:3166-3170
IPO-38 reacts with a 12-14 kDa protein, as found in Western blots of Raji cells, and appears in the mitotic cycle earlier than Ki-67. Lymphocytes, induced to early G1 phase by 12h exposure to PHA, will become positive while non-stimulated lymphocytes remain negative. Mononuclear cells and granulocytes of healthy donors are negative, while various forms of leukemia and lymphoma including Hodgkins disease are positive for IPO-38, as are many solid tumors such as some breast, gastric and colonic cancers for which it may serve as tumor progression marker.
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
IPO-38
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Sidorenko, S.P. et al., Gematol. Transfuziol. 35: 19-22 (1990)
References 2:
Sidorenko, S.P. et al., Experem. Oncol. 16: 145-150 (1994)
References 3:
Thosaporn W., et al., Oral Dis. 10(1): 22-6 (2004)
References 4:
Hao Y. et al.,J Proteome Res. Sep;7(9): 3668-77 (2008)
References 5:
Makohon N.V. et al., Fiziol Zh. 54(6): 49-57 (2008)
Mouse anti Human Prolactin antibody, clone INN-hPRL-1 recognises human prolactin, also known as luteotropic hormone or luteotropin, binding to epitope 'c' as determined by a competitive binding assay (Staindl et al. 1987). Prolactin is a 199 amnio acid ~24 kDa secreted anterior pituitary hormone acting to promote lactation.
The human progesterone receptor (PR) is expressed as two isoforms, PRA (94 kD) and PRB (114 kD), which function as ligand-activated transcription factors. These two isoforms are transcribed from distinct estrogen receptor (ER)-inducible promoters within a single copy PR gene. Clone 16 is specific for a region of the N-terminus of the A form of PR. The precise epitope has not been mapped but it reacts with both A and B forms of PR by Western blot but only with the A form by immunohistochemistry. This suggests that the epitope is inaccessible in the native folded B form of the protein.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
16
Concentration:
Greater than or equal to 324 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Hungermann D et al. Journal of Pathology 2002; 198: 487494
Mouse anti-Progesterone Receptor (A/B Forms), clone16 and SAN27
Antibody Type:
Monoclonal
Host Animal:
Mouse
Species Reactivity:
human
Immunogen:
Prokaryotic recombinant protein corresponding to the N-terminal region of the A form of the human progesterone receptor generating clone 16 and a prokaryotic recombinant protein corresponding to the 164 amino acid N-terminal region unique to the B form of the progesterone receptor generating clone SAN27.
The human progesterone receptor (PR) is expressed as two isoforms, PRA (94 kD) and PRB (114 kD), which function as ligand-activated transcription factors. In vitro studies have indicated that PRA and PRB can activate different target genes and that PRA, in some circumstances, may act as a dominant inhibitor of the function of PRB and other steroid hormone receptors. PRA and PRB are both expressed in normal breast. Most endometrial carcinomas, however, are reported to express only one isoform with either PRA or PRB being expressed. The cocktail has been formulated using two clones, clone 16, specific for PRA, and SAN27, specific for PRB.
Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
PRKAR2A (proteinkinase A regulatory type II alpha subunit), also known as PKR2, or PRKAR2, is a component of cAMP-dependent protein kinase complex. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The PRKAR2A subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER). In sperm, this antigen can be used as an intra-acrosomal marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.SpecificityThe mouse monoclonal antibody TRAP3/10 recognizes an epitope located in the C-terminal part of the intracellular domain of PRR7/TRAP3 (amino acids 126-253 of human PRR7 / TRAP3), a 28 kDa proline-rich membrane protein presumably associated with NMDA receptor complex.Application detailsImmunocytochemistry: Recommended dilution: 10 ?g/ml; membrane permeabilization (acetone) is essential.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
Hs-36
Concentration:
1 mg/ml
Format:
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Storage buffer:
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Monoclonal antibody PR3G-2 reacts with human proteinase 3 (PR3), a 30 kDa protein. PR3 is a major antigen recognized by autoantibodies directed against cytoplasmic proteins of neutrophilic granulocytes and monocytes (called anti-neutrophil cytoplasmic autoantibodies (ANCA)). ANCA are able to activate primed neutrophils to produce oxygen radicals and release lytic enzymes, including PR3. Proteinase 3 (PR3) was identified as the target antigen of ANCA in Wegener's granulomatosis (WG). ANCA directed against PR3 (PR3-ANCA) can interfere with the binding of PR3 to its physiological inhibitor alpha1-antitrypsin (alpha1-AT) and with the proteolytic activity of PR3. At the site of inflammation, PR3 can cleave the PR3-ANCA complex between these inhibiting ANCA and PR3 itself, leaving active PR3. Autoantibodies to PR3 are potent activators of the 5-lipoxygenase pathway in primed human neutrophils. Extracellular free arachidonic acid, as present at an inflammatory focus, synergizes with such autoantibodies to evoke full-blown lipid mediator generation, granule secretion and respiratory burst. Proteinase 3 (PR3) is a neutral serine proteinase, which is localized in the azurophilic granules of neutrophils and in granules of monocytes and can be detected in the membrane of secretory vesicles. PR3 degrades a number of extracellular matrix proteins such as elastin and inactivates human C1 inhibitor. Membrane-associated PR3 is also able to activate caspase-3 without triggering apoptosis of neutrophils, which is possibly a neutrophil survival mechanism. In addition, PR3 is involved in myeloid differentiation and is, therefore, also called myeloblastin. The monoclonal antibody PR3G-2 was produced by immunization of mice with a crude granule extract.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
PRG-2
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Geld van der; Y et al. Clin Exp Immunol 1999; 118: 487
PPM1D is a Mg2+/Mn2+ dependent protein phosphatase 1D with inducible expression in response to various types of environmental stress. This expression is p53-dependent, and subsequently PPM1D negatively regulates the p53-mediated transcription, thus it suppresses the apoptosis. PPM1D contributes to development of carcinomas, and seems to be a promissing therapeutic target. Amplification of PPM1D is associated with breast cancer.SpecificityThe antibody Hs-36 reacts with PRKAR2A (protein kinase A regulatory type II alpha subunit), an intra-acrosomal protein.Application detailsWestern blotting: Recommended dilution: 1-2 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
7E11/C5
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Acid extracts of boar spermatozoa were subjected to hydrophobic chromatography and the pooled fraction with reactivity to N-alpha benzoylarginine-4-nitroanilide was used for immunization.
Acrosin is a serine proteinase expressed in the acrosome of mature spermatozoa. This enzyme facilitates penetration of the sperm through the zona pellucida of the ovum.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
ACR.2
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
The monoclonal antibody 45 reacts with Polymyxin B. The antibody binds to free Polymyxin B as well as to Polymyxin B already bound to LPS. The peptide antibiotic Polymyxin B (PMB) binds to bacterial endotoxin (lipopolysaccharide, LPS). The interaction of PMB with LPS involves ionic forces between amino groups in PMB and negatively charged phosphate and carboxyl groups in the lipid A-Kdo region. PMB has relevance for endotoxin research in at least two ways: first, PMB reacts with LPS of many species regardless of varied serospecificity, and thus it can be used as a general probe for measuring or detecting LPS or lipid A. Second, binding of PMB to LPS may result in neutralization of the detrimental effects of LPS either in vitro or in vivo. Monoclonal antibody 45 enables the possibilities to study quantitatively the interaction of PMB and LPS.
Mouse anti poly (ADP-ribose) polymerase 1 antibody, clone A6.4.12 recognizes poly (ADP-ribose) polymerase 1 (PARP-1), a ~116 kDa nuclear enzyme, cleaved during apoptosis (Soldani et al. 2002). PARP-1, a caretaker enzyme, is involved in DNA damage repair (Langelier et al. 2013), plays roles in diabetes pathophysiology (Andreone et al. 2012) and tumour proliferation (Rosado et al 2013.).As well as protecting cells from genomic instability, PARP-1 is involved in the development of both inflammatory and immune responses, and cell death by apoptosis and necrosis (Erdélyi et al. 2005). Mouse anti poly(ADP-ribose) polymerase 1 antibody, clone A6.4.12, targets PARP-1, an enzyme which represents a promising target for new developments in therapeutic treatment of immune mediated diseases (Rosado et al. 2013). PARP-1 has considerable potential for delivering selective tumour cell killing while sparing normal cells (Pinton et al. 2013).
Polo-Like Kinase-1 (PLK1) also known as Serine/Threonine Protein Kinase 13 is a 66 kDa kinase. The activity of PLK-1 is crucial for mitosis and maintenance of genome stability. PLK-1 localizes to centrosomes and kinetochores where it plays a key role in late prophase and prometaphase. PLK-1 is overexpressed in many types of cancers and mediates estrogen receptor-mediated gene transcription in breast cancer cells. Overexpression of PLK-1 is associated with tumor development, with elevated levels of expression reported in non-small cell lung cancers, head and neck, gastric, breast, ovarian, colon and several other cancer types.
Podoplanin is a transmembrane mucoprotein (38 kD) recognized by the D2-40 monoclonal antibody. Podoplanin is selectively expressed in lymphatic endothelium as well as lymphangiomas, and Kaposi sarcomas. Podoplanin has also been shown to be expressed in epithelioid mesotheliomas and seminomas.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN
Clone:
D2-40
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Ordóñez NG. Adv Anat Pathol. 2006; 13:83-8
References 2:
Niakosari F, et al. Arch Dermatol. 2005; 141:440-4
Pneumocystis carinii is a fungal organism which is detected in human tissues (typically in lung in immunocompromised patients) in the trophozoite stage. Anti-Pneumocystis carinii antibody reacts with an epitope on the organism which is resistant to formalin, picric acid, paraffin, as well as alchohol and xylene. No cross-reactivity has been demonstrated with other fungi or parasitic organisms.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN Ready To Use
Clone:
3F6
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Anti-PLAP antibody immunoreacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease. This antibody has shown cross-reaction with human intestinal alkaline phosphatase.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN Ready To Use
Clone:
NB-10
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Paiva, J, et al. Am J Pathol 1984;111:156-165
References 2:
Burke, AP, et al. Hum Path 1988;19:663-670
References 3:
Wick, MR, et al. Hum Path 1987;18:946-954
References 4:
Saad RS et al. Appl Immunohistochem Mol Morphol. 2003 Jun;11(2):107-12
References 5:
Goldsmith JD et al. Am J Surg Pathol. 2002 Dec;26(12):1627-33
Plakoglobin, also known as gamma-catenin belongs together with alpha- and beta catenin to the catenin family. Catenins mediates cell-cell adhesion by interaction with cadherins. Plakoglobin is found in desmosomes and adherens junctions. Plakoglobin is highly homologous to beta-catenin although its function differs from that of beta-catenin. Whereas beta-catenin has been found in potentiating hyperproliferation and tumor formation, plakoglobin can suppress tumorigenicity. Overexpression of plakoglobin has been shown to suppress cell proliferation and cell tumorigenicity in animals. Furthermore reduced plakoglobin expression has been found in tumor tissues and metastatic lesions of renal cells, esophageal carcinomas and in skin carcinomas. The monoclonal antibody 15F11 cross reacts with rat and weakly with mouse.
PLAP is a tissue specific, throphoblast-derived, 58 kDa, glycosyl-phosphatidylinositol (GPI)- anchored, dimeric, Zn2+ metallated glycoprotein, only found in humans, orangutans and chimpanzees, that catalyzes the hydrolysis of phosphomonoesters into an inorganic phosphate and an alcohol. It is present in the placenta and serum of pregnant women and in high frequency in gynecological and testicular cancers and in lower frequency in other tumors. The three tissue-specific APs in humans, PLAP, germ cell AP (GCAP) and intestinal AP, are 90-98% homologous. Non tissue specific AP is found in kidney, liver and bone. H7E8 binds equally well to all common allelic variants (S,F, FS and I) of PLAP as to AP from normal human testis, while antibody F5C2 reacts with some samples of normal human testis only.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
H7E8
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Millan J.L. et al, Eur. J. Biochem. 136: 1-7 (1983)
PLAP is a tissue specific, throphoblast-derived, 58 kDa, glycosyl-phosphatidylinositol (GPI)- anchored, dimeric, Zn2+ metallated glycoprotein, only found in humans, orangutans and chimpanzees, that catalyzes the hydrolysis of phosphomonoesters into an inorganic phosphate and an alcohol. It is present in the placenta and serum of pregnant women and in high frequency in gynecological and testicular cancers and in lower frequency in other tumors. The three tissue-specific APs in humans, PLAP, germ cell AP (GCAP) and intestinal AP, are 90-98% homologous. Non tissue specific AP is found in kidney, liver and bone. F5C2 binds equally well to all common allelic variants (S,F, FS and I) of PLAP and to some variants of AP from normal human testis, while antibody H7E8 reacts with all variants of AP in normal human testis.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
F5C2
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Millan J.L. et al, Eur. J. Biochem. 136: 1-7 (1983)
Placental alkaline phosphatase (PLAP) is a membrane-associated sialoglycoprotein enzyme normally present at high concentration in syncytiotrophoblasts within the placenta during the third trimester of gestation. The expression of PLAP was originally thought to be restricted to term placenta but a human PLAP-like variant has been described which shares more than 85% homology with PLAP itself. This high degree of homology between PLAP and PLAP-like enzyme together with cross-reacting antibodies has led to some confusion of the distribution of PLAP and PLAP-like enzyme in various tissues. PLAP is reported to be expressed only in normal term placenta, endocervix and fallopian tube and also in ovarian and proximal gastrointestinal tumors. PLAP expression is rare in malignant germ cell tumors. PLAP-like enzyme is reported to be predominantly found in normal fetal and neonatal testis, and in thymus. It is also commonly expressed in germ cell tumors and more recently described in seminomas.
Antibody Isotype:
IgG1, kappa
Monosan Range:
MONXtra
Clone:
8A9
Concentration:
Greater than or equal to 26 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Bartkova J et al. Oncogene. 2000; 19: 4146-4150
References 2:
Franke FE et al. Human Pathology 2000; 31(12), 14661476
References 3:
Hoei-Hansen CE et al. Molecular Cancer. 2007; 6:12
References 4:
McCann-Crosby B et al. International Journal of Pediatric Endocrinology 2015; 1:14
Perforin is a 70 kDa cytolytic protein with structural and functional similarities to complement component 9 (C9). It is stored in cytoplasmic granules of cytotoxic T cells and NK cells and after its release it forms transmembrane pores in the target cells to kill it. As perforin is a key effector molecule for cell-mediated cytolysis, defects of its gene can cause severe disorders.SpecificityThe mouse monoclonal antibody 7E11/C5 recognizes an epitope within the sequence FTNEDELYNLLTDSP of PPM1D, a protein phosphatase, which contributes to development of some carcinomas.Application detailsFlow cytometry: Recommended dilution: 3-12 ?g/ml. Intracellular staining.
Antibody Isotype:
IgG2b kappa
Monosan Range:
MONOSAN
Clone:
dG9
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Perforin is a pore-forming protein that leads to osmotic lysis of the target cells and subsequently enables granzymes to enter the target cells and activate apoptosis, the cell death program. The expression of perforin is reportedly upregulated in activated CD8+ T-cells, natural killer cells and some CD4+ T-cells.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN Ready To Use
Clone:
MRQ-23
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Chu PG, et al. Ann Diagn Pathol. 1999; 3:104-33
References 2:
Bittmann I, et al. Arch. 2004; 445:375-81
References 3:
dAmore ES, et al. Pediatr Dev Pathol. 2007; 10:181-91
The antibody is specific to human pepsinogen II and has no crossreactivity to human pepsinogen I. Pepsinogen II is a group of precursor molecules for pepsin. These proteins are secreted into the gastric lumen by the pyloric glands of the gastric antrum and also by the chief and neck cells of the gastric corpus (oxyntic mucosa). Negative immunohistochemical reaction for pepsinogen I (right) but positive reaction for pepsinogen II (left) is a typical sign of the antral mucosa and, in the presence of atrophic gastritis, this staining pattern indicates that the positive glands and cells are metaplastic and pyloric in differentiation (so called pseudopyloric metaplasia).
The antibody is specific to human pepsinogen I and has no crossreactivity to human pepsinogen II. Pepsinogen I is a group of precursor molecules for pepsin. These proteins are solely synthetized and secreted into gastric lumen by chief (pepsin) cells and mucous neck cells in the gastric corpus (oxyntic mucosa). In atrophic corpus gastritis these cells disappear resulting in a decrease of the serum level of pepsinogen I and in a reduction of the number of pepsinogen I positive cells in gastric biopsies. The presence of positive immunostaining for pepsinogen I is a highly reliable sign for the acid-secreting oxyntic glands. In gastric heterotopia of the duodenal bulb, but not in gastric metaplasia, the oxyntic-type glands give a positive immunohistochemical reaction for pepsinogen I.
Pen 9 reacts mainly with the thiazolidin ring of penicillin, but not with the lactam ring. The nature of the side chain in the penicilloyl group does not affect antibody binding as was shown by testing Pen 9 against benzylpenicillin, ampicillin, amoxillin and 6-aminopenicillanic acid. The presence of carrier protein is not essential for the presentation of the antigen associated with Pen 9.
Antibody Isotype:
IgG1-kappa
Monosan Range:
MONOSAN
Clone:
Pen 9
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
P. de Haan et al., Int. Archs Allergy appl. Immun. 76: 42-46 (1985)
This monoclonal antibody binds to human PECAM-1 (platelet endothelial cell adhesion molecule-1; CD31) a specific component of endothelial cell junctions. PECAM-1 is also expressed in platelets and leukocytes.
The programmed death receptor 1 (PD-1) protein is a cell-surface receptor on certain lymphocytes that, with its ligand programmed death ligand 1 (PD-L1), helps to down-regulate immune responses. Many cancer types express PD-L1 and evade immune recognition via the PD-1/PD-L1 interaction. Precision therapies targeting the PD-1/PD-L1 pathway have the potential to improve response and thereby offer a novel treatment avenue to some patients with cancer.
Programmed death-1 (PD-1), also known as CD279, is a type-I transmembrane protein expressed on T-cells, B-cells, and monocytes during activation.1-2 PD-1 and its ligands (PD-L1 and PD-L2) function as an immune checkpoint pathway by regulating T-cell activation and autoimmunity.2 PD-1 labels follicular helper T-cells and is therefore a useful marker for angioimmunoblastic T-cell lymphoma.3-4
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN Ready To Use
Clone:
NAT105
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Bolstad AI, et al. Arthritis Rheum. 2003 Jan;48(1):174-85
References 2:
Dorfman DM, et al. Am J Surg Pathol. 2006 Jul;30(7):802-10
References 3:
Hamanishi J, et al. Proc Natl Acad Sci U S A. 2007 Feb 27;104(9):3360-5
References 4:
Konishi J, et al. Clin Cancer Res. 2004 Aug 1;10(15):5094-100
References 5:
Mataki N, et al. Am J Gastroenterol. 2007 Feb;102(2):302-12
PCNA (proliferating cell nuclear antigen) is the DNA polymerase delta auxiliary protein acting in homotrimeric form to increase the processivity of leading strand synthesis during DNA replication. PCNA is expressed in the nucleus of all proliferating cells. In response to DNA damage, it is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. PCNA is a useful marker of DNA synthesis, as its form not involved in DNA synthesis degradates in histological preparations in the presence of organic solvents.SpecificityThe mouse monoclonal antibody dG9 (also known as deltaG9) recognizes perforin, a 70 kDa protein expressed in cytoplasmic granules of cytotoxic T cells and NK cells.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml. Intracellular staining.
Antibody Isotype:
IgG2a kappa
Monosan Range:
MONOSAN
Clone:
PC10
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
PCLO (piccolo, also known as aczonin) is a large (more than 400 kDa) multidomain protein of the presynaptic cytomatrix in neurons, that is present in all vertebrate synapses, but is absent from invertebrates. It contains zinc finger and coiled-coil sequences, as well as N-terminal glutamine-rich sequence, and C-terminal PDZ domain followed by two C2 domains (C2A and C2B). In vitro binding and transfection experiments suggested that PCLO binds to multiple proteins including profilin and L-type calcium channels. It is involved in neurotransmitter release and insulin secretion.SpecificityThe mouse monoclonal antibody PC10 (also known as 3F81) recognizes PCNA, a 36 kDa conserved nuclear protein serving as a cofactor for DNA synthesis.Application detailsFlow cytometry: Recommended dilution: 1-5 ?g/ml, intracellular staining.<br>Western blotting: A small splice variant of PCLO can be analyzed by Western blotting, however, the whole protein is too large (over 400 kDa) to be successfully processed by SDS PAAGE.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
PCLO-01
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
PAX8 antibody recognizes a protein of 62kDa, identified as PAX8. It is a member of the paired box (PAX) family of transcription factors. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas, and atypical thyroid adenomas. PAX8 is expressed in the thyroid (and associated carcinomas), non-ciliated mucosal cells of the fallopian tubes, and simple ovarian inclusion cysts, but normal ovarian surface epithelial cells. PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas. PAX8 antibody may be used as an additional immunohistochemical marker for renal epithelial tumors. Pre-treatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0 for 20 minutes, is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based detection is recommended. Control tissue Renal Cell Carinoma. Staining Nuclear
PAX-8 is a transcription factor expressed during embryonic development of Müllerian organs, kidney, and thyroid, with continued expression in some epithelial cell types of these mature tissues.1 It can be useful for marking several types of carcinoma including ovarian serous carcinoma, clear cell renal cell carcinoma, and papillary thyroid carcinoma.1-5 Additionally, PAX-8 is not found in the epithelial cells of the breast, lung, mesothelium, stomach, colon, pancreas and other sites.
Antibody Isotype:
IgG
Monosan Range:
MONOSAN
Clone:
MRQ-50
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Ozcan A, et al. Mod Pathol. 2011; 24:751-64
References 2:
Laury AR, et al. Am J Surg Pathol. 2011; 35:816-26
References 3:
Nonaka, D et al. Am J Surg Pathol. 2008; 32:1566-71
The PAX-5 gene is essential for B-cell differentiation. There are at least four isoforms, of which PAX-5a has been most studied. PAX-5 encodes the 50 kDa B-cell specific activator protein, BSAP. PAX-5 is expressed by pro-, pre-and mature B-cells, but is downregulated during terminal differentiation of plasma cells. PAX-5 influences the expression of other B-cell specific genes, including CD19 and CD20 and CD79a, preceding the expression of CD20. PAX-5 is silenced at the plasma cell stage under the influence of B-lymphocyte-induced maturation protein-1 (PRDM1). PAX-5 is expressed during mouse embryogenesis within the developing brain in a way that is temporarily and spatially tightly condoled. PAX-5 deficient mice show deformation of the mid-brain. Expression in human embryogenesis occurs in the mesoencephalon and spinal cord. Pretreatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0, for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. (dilution 1:100 - 1:200) The optimal dilution for a specific application should be determined by the investigator. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based detection is recommended. Control tissue Tonsil; staining nuclear
PAX-5 encodes for B-cell-specific activator protein (BSAP), a marker for B-cells, including B-lymphoblastic neoplasms and maturation stage. It is found in most cases of mature and precursor B-cell non-Hodgkin lymphomas/leukemias. In approximately 97% of cases of classic Hodgkin lymphoma, Reed-Sternberg cells express PAX-5.4 PAX-5 is not detected in multiple myeloma and solitary plasmacytoma, making it useful for such differentiation.1,3 Diffuse large B-cell lymphomas do express PAX-5, save for those with terminal B-cell differentiation. T-cell neoplasms do not stain with anti-PAX-5. There is a strong association with CD20 expression.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN Ready To Use
Clone:
24
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Torlakovic E, et al. Am J Surg Pathol. 2002; 26:1343-50
Pax genes are a family of developmental control genes that encode nuclear transcription factors and have been implicated in the control of mammalian development. Pax-5 is a B cell specific transcription factor that is expressed in pro B cells, pre-B and mature B cells, and subsequently in all stages of B cell development until the plasma cell stage in which it is downregulated.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
1EW
Concentration:
Greater than or equal to 29 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Hansson M et al. European Journal of Haematology. 2007; 79:159-165
The antibody targets the capsid proteins VP1 and VP2 on Human Parvovirus. Parvovirus B19 infection has been implicated as a cause in spontaneous abortion in humans and thus application of this antibody to placental tissues in such cases is appropriate. Parvovirus B19 is also associated with erythema infectiosum (fifth disease) in children and acute arthritis in adults, as well as chronic hemolytic anemia, with some patients experiencing aplastic crisis.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN Ready To Use
Clone:
R92F6
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Loughrey AC et al. J Med Vir 39:97-100 (1993)
References 2:
Moore L et al. Med J Australia 159:344-345 (1993)
References 3:
Morey AL et al. J Path 166:105-108 (1992)
References 4:
ONeill HJ et al. 123: 125-134 (1992)
References 5:
Silverberg SG et al. Principles and Practice of Surgical Pathology and Cytopathology, 3rd edition. 1997; p. 219-220
The parathyroid glands are small, oval, endocrine glands closely associated with the thyroid gland. The parathyroid glands regulate serum calcium and phosphate levels via parathyroid hormone (parathormone). Parathyroid hormone raises serum calcium levels directly, by increasing the rate of osteoclastic reabsorption and promoting breakdown of the bone matrix, and indirectly, by increasing the renal tubular reabsorption of calcium ions and inhibiting the reabsorption of phosphate ions from the glomerular filtrate, and finally, by promoting the absorption of calcium from the small intestine. Parathyroid hormone is the most important regulator of blood calcium levels and is essential to life, whereas calcitonin appears only to provide a complementary mechanism for fine adjustment. Chief cells are the most abundant cells in the parathyroid gland and are responsible for the secretion of parathyroid hormone.
Antibody Isotype:
IgG2a
Monosan Range:
MONXtra
Clone:
105G7
Concentration:
Greater than or equal to 12 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Au AYM et al. The New England Journal of Medicine, 2008 359;(11):1184-1186
References 2:
Ikeda K et al. Diagnostic Cytopathology. 2005:34(1):50-55
The antibody does stain all types of keratin containing (that is epithelial) cells in frozen sections of various tissues, with the exception of myo-epithelial cells and the podocytes of the glomeruli.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
80
Concentration:
15 ug/ml
Storage buffer:
Culture medium with 0.01M Sodium Azide
Storage:
2-8°C
References 1:
Muijen GNP van (1984) Am J Pathol 114, 9
References 2:
Muijen GNP van (1984) Am J. Pathol 116, 363
References 3:
Muijen GNP van (1985) Hum Pathol 16, 590
References 4:
Corver WE et al. (2000) Cytometry 41, 73-80
References 5:
Broek, JCM van et al. (2000) Appl. Immunohis. & Mol. Morph. 8, 316-321
Cytokeratins are classified into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant carcinomas.
Plasminogen activator inhibitor type-1 (PAI-1), a member of the serine protease inhibitor (serpin) superfamily, is an important protein in the regulation of fibrinolysis. PAI-1 is unique among the serpins because of its functional and conformational flexibility. PAI-1 is the most important physiological inhibitor of both tissue-type plasminogen activator (t-PA) and urokinase-type plasminogen activator (u- PA). Increased PAI-1 levels are associated with thrombotic events and is an established risk factor for cardiovascular diseases. The active conformation PAI-1 inhibits its target proteinases by the formation of a stable, inactive complex. Although PAI-1 is synthesized as an active molecule, it converts spontaneously to an inactive, latent form that can be partially reactivated by denaturing agents. In addition, a third conformation reacting as a non-inhibitory substrate towards various target proteinases has been identified.<br /> The epitope of monoclonal antibody MA-33H1F7 is predominantly composed of three residues (Lys154/Glu130/Arg131), positioned virtually linearly in the three-dimensional structure. The epitope of the antibody does not cover the complete alpha-helix F and turn connecting alpha-helix F and beta-strand s3A, but is restricted to the hinge region between alpha-helix F and the main part of the PAI-1 molecule.<br /> The monoclonal antibody MA-33H1F7 is a âswitchingâ antibody, capable of inducing a non-inhibitory substrate form of PAI-1. It was shown to inhibit PAI-1 in a dose dependent manner.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MA-33H1F7
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Debrock; S et al. Biochim Biophys Acta 1997; 1337: 257
PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
MEM-255
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Prokaryotic recombinant protein corresponding to a region which spans the tyrosine kinase catalytic domain and part of the C-terminus of the NPM-ALK transcript (419-520??).
Anaplastic large cell lymphoma (ALCL) is usually composed of large pleomorphic cells which are reported to express CD30 antigen and epithelial membrane antigen (EMA). These tumor cells tend to occur in younger individuals and may be associated with cutaneous and extranodal involvement. A proportion of these cases contain a chromosomal translocation t(2;5) (p23;q35). This results in a hybrid gene encoding part of the nucleophosmin (NPM) gene joined to the cytoplasmic domain of the anaplastic lymphoma kinase (ALK) gene, giving rise to the protein, p80. Large cell lymphomas account for approximately 25% of all non-Hodgkin's lymphomas in children and young adults, of which one third carry the NPM-ALK gene translocation.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
5A4
Concentration:
Greater than or equal to 32 mg/L
Storage buffer:
Tissue culture supernatant with 15mM sodium azide
Storage:
2-8°C
References 1:
Liu A et al. Acta Histochemica et Cytochemica. 2004; 37(1):21-30
The p53 family of proteins includes three members, p53, p63, and p73. The protein p63 is encoded by TP63 gene, which gives rise to protein isoforms with different properties and functions due to the presence (TAp63) or absence (deltaNp63) of an N-terminal transactivation domain. Immunohistochemistry of p63 has a clinical relevance for certain tumor types, but investigations have been hampered by a lack of well characterized antibodies that are specific for p63 and do not cross-react with the related p53 and p73 proteins.SpecificityThe mouse monoclonal antibody PCLO-01 recognizes PCLO (Piccolo), a more than 400 kDa multidomain protein expressed mainly in the presynaptic cytoplasmatic matrix of the neurons.Application detailsWestern blotting: Recommended dilution: 1 ?g/ml
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
PANp63-6.1
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
p63 is a type II integral membrane protein predominantly localized in the rough endoplasmic reticulum. p63 is reported to be expressed in a number of normal tissues including proliferating cells of the epithelium, cervix, urothelium and prostate. p63 is also reported to be expressed in most poorly differentiated squamous cell carcinomas.
Antibody Isotype:
IgG1, kappa
Monosan Range:
MONXtra
Clone:
7JUL
Concentration:
Greater than or equal to 208 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Mahalingam M et al. Modern Pathology. 2010; 23:713-719
References 2:
Shah VI et al. Histopathology. 2006; 48:683-691
References 3:
Yen CC et al. World Journal of Gastroenterology. 2005; 11(9):1267-1272
References 4:
Bilal H et al. The Journal of Histochemistry and Cytochemistry. 2003; 51(2):133-139
p63 is a homolog of the tumor suppressor p53. It is identified in basal cells in the epithelial layers of a variety of tissues, including epidermis, cervix, urothelium, breast and prostate. p63 was detected in nuclei of the basal epithelium in normal prostate glands; however, it was not expressed in malignant tumors of the prostate. As a result, p63 has been reported as a useful marker for differentiating benign from malignant lesions in the prostate, particularly when used in combination with markers of high molecular weight cytokeratins and the prostate-specific marker AMACR (P504S). p63 has also been shown to be a sensitive marker for lung squamous cell carcinomas (SqCC), with a sensitivity of ~90%. Specificity for lung SqCC, vs. lung adenocarcinoma (LADC), is approximately 80%. In breast tissue, p63 has been identified in myoepithelial cells of normal ducts. Pretreatment: Heat induced epitope retrieval in 10 mM citrate buffer, for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based detection is recommended. Control tissue Breast, Prostate, Prostate carcinoma or lung or bladder squamous cell carcinoma
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
TP63/11
Concentration:
n/a
Format:
Concentrate
Storage buffer:
Bioreactor Concentrate with 0.05% Azide
Storage:
2-8°C
References 1:
Yang A, et al. Mol Cell 1998;2:305-16
References 2:
Signoretti S, et al Am J Pathol 2000;157:1769-75
References 3:
Yang A, et al. Nature 1999;398:714-18
References 4:
Barbareschi M. et al. Am J Surg Pathol 2001 Aug;25(8);1054-60
References 5:
Werling RW, et al. Am J Surg Pathol 2003 Jan;27(1):82-90
Cyclin-dependent kinases are positive regulators of cell proliferation. p57 protein acts as a tumor suppressor to counter this. It is closely related to other CDKIs such as p21 protein (CIP1) and p27 protein (Kip1) as they share a common structural N-terminal domain for binding to CDK/cyclin complexes and inhibiting their kinase activity. Human p57 protein is found on chromosome 11p15.5, a region which is reported to be a common site for loss of heterozygosity in certain sarcomas, Wilms tumors and tumors associated with the Beckwith-Wiedermann syndrome. There is increasing interest in p57 as a marker in gestational disease. Gestational trophoblastic disease refers to a spectrum of proliferative disorders of the placental trophoblast, with a wide range of histologic appearances and clinical behaviors.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
25B2
Concentration:
Greater than or equal to 19 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Kanthan R et al.World Journal of Surgical Oncology 2010; 8:10
References 2:
Sharifi N et al.Journal of the Turkish-German Gynecological Association 2009;10:39-42
References 3:
Maggiori MS and Peres LC. European Journal of Obstetrics and Gynecology and Reproductive Biology 2007; 135: 170-176
The antibody has been used as an aid in discriminating complete hydatidiform mole (CHM) (no nuclear labeling of cytotrophoblasts or stromal cells) from partial hydatidiform mole (PHM) (nuclear staining of both cytotrophoblasts and stromal cells) and hydropic abortion. In normal placenta, cytotrophoblast, syncytio trophoblast, and stromal cells are labeled with this antibody. Intervillous trophoblastic islands demonstrate nuclear labeling in all entities and serve as an internal control.
Antibody Isotype:
IgG2b-k
Monosan Range:
MONOSAN Ready To Use
Clone:
KP10
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Kihara M, et al. J Reprod Med. 2005; 50:307-12
References 2:
Romaguera RL, et al. Fetal Pediatr Pathol. 2004; 23:181-90
This monoclonal antibody recognizes both wild type and mutant forms of human p53 protein under denaturing and non-denaturing conditions. The epitope recognized by clone DO-7 can be destroyed by prolonged fixation in buffered formalin. The heat induced epitope retrieval technique may improve staining in some cases.
Antibody Isotype:
IgG2b
Monosan Range:
MONXtra
Clone:
DO-7
Concentration:
Greater than or equal to 22 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Tiniakos DG et al. Cytopathology. 1996; 7(3): 178186
References 2:
Yoshida T et al. Journal of Pathology. 2003; 199(2):166175
References 3:
Burns ASYW et al. British Journal of Cancer. 2002; 86(7):11171123
References 4:
Tweddle DA et al. American Journal of Pathology. 2001; 158(6): 20672077
References 5:
Fernando SS et al. International Journal of Surgical Pathology. 2000; 8(3):213222
PAb 122 binds to the C-terminus (aa370-378) of both wild type and mutated p53. When microinjected into nuclei, PAb 122 blocked re-entry into the S-phase of the cell cycle. Mutation and/or allelic loss of p53 is one of the causes of a variety of mesenchymal and epithelial tumors. p53 Localizes in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution.
The antibody recognizes a 53 kDa phosphoprotein, identified as p53 suppressor gene product. It reacts with the mutant as well as wild type p53.1 Positive nuclear staining with this antibody has been shown to be a factor in breast carcinoma, lung carcinoma, colorectal carcinoma, urothelial carcinoma, and ependymoma.2-8 Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma, as well as a marker for intratubular germ cell neoplasia.
Antibody Isotype:
IgG2b-k
Monosan Range:
MONOSAN Ready To Use
Clone:
DO7
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Mauri FA et al. Int J Oncol 1999 Dec;15(6):1137-47
References 2:
Caffo O et al. Clin Cancer Res 1996 Sep;2(9):1591-9
References 3:
Bebenek M et al. Anticancer Res 1998 Jan-Feb;18(1B):619-23
References 4:
Midulla C et al. Anticancer Res 1999 Sep-Oct;19(5B):4033-7
References 5:
Moore BE et al. App.IHC and Mol. Morphol. 2001;9(3): 203 206
The gene coding for p53 oncoprotein is located on chromosome 17p. Allele loss at this chromosome site has frequently been seen in many tumours including lung, colon, breast and brain. Expression of p53 oncoprotein was not detected in normal mucosa, whereas mutation results in a detectable expression of the p53 protein. It is therefore suggested that immunocytochemical detection of p53 protein is an indication for malignancy. Positive control: Breast carcinoma.
The tumour suppressor protein p53 is a key element of intracellular anticancer protection. It mediates cell cycle arrest or apoptosis in response to DNA damage or to starvation for pyrimidine nukleotides. It is up-regulated in response to these stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest, or proteins that trigger apoptosis, such as Bcl-2. The structure of p53 comprises N-terminal transactivation domain, central DNA-binding domain, oligomerisation domain, and C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
BP53-12
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Bp53-12 reacts with an N-terminal epitope (aa 16-25) of both wild-type and mutated p53. This epitope is revealed in tissue sections only after formalin fixation. Mutation and/or allelic loss of p53 is one of the causes of a variety of mesenchymal and epithelial tumors. p53 Localizes in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution.
Antibody Isotype:
IgG2a-A
Monosan Range:
MONOSAN
Clone:
Bp53-12
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bártek J. et al, J Pathol. 169(1):27-34 (1993)
References 2:
Vogelstein and Kinzler, Cell 70: 523-526, (1992)
References 3:
Hollstein et al, Science 253: 49-53: (1991)
References 4:
Lane, D.P, Nature 358: 15-16: (1992)
References 5:
Donehower et al, Biochemic. Biophys. Acta 1155: 181-182, (1993)
Cdk1 (cyclin-dependent kinase 1), also known as p34Cdc2 (cell division control protein kinase 2) depends on cyclin A and B and is triggered by a positive feedback loop at the end of G2 phase, which is the key event that initiates mitotic entry. Destruction of cyclin B during metaphase results in inactivation of Cdk1, allowing mitotic exit and cell division. Cdk1 also contributes to the control of DNA replication. Cdk1 can be ihibited by several transcriptional targets of p53, such as p21WAF.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
POH-1
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
p27, also known as cyclin-dependent kinase inhibitor 1B (CDNK1B), is a kinase inhibitor that controls cell cycle progression.1-4 p27 is involved in G1 phase arrest and obstructs cell entry into the S phase by binding to and inhibiting cyclin E-CDK2, effectively slowing or stopping the cell division cycle.1-4 p27 is broadly expressed in normal tissue but can be dysfunctional in neoplastic tissue and, therefore, not expressed.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN Ready To Use
Clone:
SX53G8
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
The tumour suppressor p21Waf1 (wild-type p53-activated fragment 1; also known as Cip1, Cdk interacting protein, or SDI 1) is a cyclin-dependent kinase (Cdk) inhibitor, which is expressed by involvement of p53, Egr-1, AP2, STATs or other transcription factors upon various stimuli resulting in cell cycle arrest. Through its N-terminal domain p21Waf1 inhibits Cdk activity, whereas through the C-terminal domain it inhibits the activity of PCNA (proliferating cell nuclear antigen) to activate DNA replication. Cytosolic location of p21 counteracts its inhibitory activities.SpecificityThe mouse monoclonal antibody PANp63-6.1 recognizes both TAp63, and deltaNp63 form of p63. The target epitope PSHLIR is located within amino acids 261-266 of TAp63, and 167-172 of deltaNp63.Application detailsImmunohistochemistry (paraffin sections): Positive tissue: colon carcinoma.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
WA-1
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
p193-6 reacts with an internal epitope (amino acids 593-599, FSKVEDY) of the minor vault protein (p193 or VPARP), which is overexpressed in various human non-P-glycoprotein MDR tumor cell lines, accordingly to an increase in the number of vault particles. p193-6 was raised against an E. coli lysate transformed with the pET28a(+) expression vector containing amino acids 408-611 of the p193 cDNA
p193-4 reacts with an internal epitope (amino acids 491-494, HPGE) of the minor vault protein (p193 or VPARP), which is overexpressed in various human non-P-glycoprotein MDR tumor cell lines, accordingly to an increase in the number of vault particles. p193-4 was raised against an E. coli lysate transformed with the pET28a(+) expression vector containing amino acids 408-611 of the p193 cDNA.
p193-10 reacts with an internal epitope (amino acids 506-510, VALGK) of the minor vault protein (p193 or VPARP), which is overexpressed in various human non-P-glycoprotein MDR tumor cell lines, accordingly to an increase in the number of vault particles. p193-10 was raised against an E. coli lysate transformed with the pET28a(+) expression vector containing amino acids 408-611 of the p193 cDNA.
P16 is a mitotic inhibitor protein. It competes with D-type cyclins to bind to cdk4 and cdk6. It acts as tumor suppressor and inhibits the progression of cells through the G1 phase of the cell cycle. Positive Control Tissue Uterine cervical squamous cell carcinoma
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
JC2
Concentration:
lot specific
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Sherr et al. Cold Spring Harb Symp Quant Biol 1994;59:11-19
As one of the cyclin-dependent kinase inhibitors that inhibit cylcin-dependent kinases 4 and 6, p16INK4A is encoded by tumor suppressor gene CDKN2A. The tumor suppressor p16INK4A plays an important role in cell cycle regulation. Increased expression of the p16 gene, which is seen as organisms age, reduces the proliferation of stem cells. This reduction in the division and production of stem cells protects against cancer while increasing the risks associated with cellular senescence. Mutations in the p16 gene associated with loss or over expression of the protein are associated with increased risk of a wide range of cancers and cancer precursor lesions. The Immunohistochemical identification of p16 is particularly relevant in uterine cervical lesions: Development of dysplasia is closely related to human papilloma virus (HPV) infection. Pretreatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0, for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections.Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based detection is recommended. Control tissue Human cervical cancer, tonsil. Staining cytoplasmic and nuclear.
p16 (CDKN2A, p16Ink4A), is key regulator of the cell cycle and involved in cell cycle control and cellular senescence. It is a specific inhibitor for Cdk4 and Cdk6 and binds to the phosphorylated Cdk-cyclin complex. A disruption of this pathway is commonly observed in cancer. p16 is lost in the majority of tumor cell lines and in most primary tumors. It is not expressed in melanoma.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
DCS-50
Concentration:
n/a
Storage buffer:
Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage:
2-8°C
References 1:
Wiest, T. et al. Oncogene 2002;21: 15101517
References 2:
Shibata, K. R. et al. Stem Cells 2007; 25: 237182
p120 catenin is encoded on chromosome 11q11. Alpha-catenin and beta-catenin bind to the intracellular domain of E-cadherin while p120 catenin binds E-cadherin at a juxta-membrane site. The complex stabilizes tight junctions. In the cell, p120 catenin localized to the E-cadherin/catenins cell adhesion complex, directly associates with cytoplasmic C-terminus of E-cadherin and may similarly interact with other cadherins. A deficiency of E-cadherin results in the intracytoplasmic accumulation of p120 catenin. Lobular carcinoma of the breast shows intracytoplasmic accumulation of p120 catenin while ductal carcinoma shows reduced membrane p120 catenin without cytoplasmic accumulation. In gastric and colonic carcinoma, strong cytoplasmic p120 catenin is associated with discohesive infiltrative morphology.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN Ready To Use
Clone:
MRQ-5
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Reynolds AB, et al. Oncogene.; 7:2439-45 (1992)
References 2:
Thoreson MA, et al. J Cell Biol.; 148:189-202 (2000)
References 3:
Sarrio D, et al. Oncogene.; 23:3272-83 (2004)
References 4:
Dabbs DJ, et al. Am J Surg Pathol.; 31:427-37 (2007)
LRP-56 reacts with an internal epitope of the LRP-protein (P110), which is strongly over-expressed in various human non-P-glycoprotein MDR tumor cell lines.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
LRP-56
Concentration:
100 ug/ml
Storage buffer:
supernatant with 1% BSA and 0.1% sodium azide
Storage:
2-8°C
References 1:
Scheper RJ et al. Int.J.Cancer 1993; 53: 1475-1479
References 2:
List AF et al. Blood 1993; 82: 443a
References 3:
Izquierdo MA et al. ProcAACR 1993; 34: 311
References 4:
Scheper RJ et al. Proc.Am.Ass Cancer Res. 1994; 54: 4557-4563
References 5:
Scheffer GL et al. Proc Am Ass Cancer Res 1995; 36: 323
The antibody stains 80% of non-mucinous primary and metastatic ovarian cancers. This monoclonal antibody is used for the identification of primary and metastatic non-mucinous ovarian carcinoma and ovarian carcinoma cells in peritoneal fluids. It rarely stains nongynaecological malignancies.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
OV632
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.01M Sodium Azide
Storage:
2-8°C
References 1:
Fleuren GJ et al. (1987) Virchows Archiv A, 410, 481-486
References 2:
Boerman OC et al. (1991) Int J Gynecol 10, 15-23
References 3:
Delahye H et al., (1991) J Pathol 165 137-143
References 4:
Boerman OC et al. (1991) Anticancer Res 10, 1289-1296
OPAL1 (outcome predictor in acute leukemia 1) is an almost uncharacterized transmembrane adaptor protein expressed mainly by megacaryocytes and platelets. Its expression is enhanced on TEL/AML leukemic cells. The function of OPAL1 is unknown, although the presence of a cytochrome c-like heme-binding site and a transmembrane domain suggested OPAL1 may be involved in the mitochondrial electron transport chain. Its originally reported prognostic impact appears to be treatment dependent.SpecificityThe antibody WA-1 reacts with p21 protein (Waf1, Cip1, SDI 1; intracellular antigen), a 21 kDa tumour suppressor, which inhibits the activity of cyclin-dependent family kinases and of proliferating cell nuclear antigen.Application detailsFlow cytometry: Recommended dilution: 1-4 µg/ml, intracellular staining.<br>Western blotting: Recommended dilution: 1-2 ?g/ml, non-reducing conditions.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
OPAL1-01
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Oct-4 is a transcription factor that functions in the regulation and maintenance of pluripotency in embryonic stem and primordial germ cells. Oct-4 immunoreactivity has been demonstrated in gonadal and extra-gonadal seminomas, dysgerminomas and embryonal carcinomas. In addition, the immunohistochemical detection of Oct-4 assists in the evaluation of intratubular germ cell neoplasia (IGCN).
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN Ready To Use
Clone:
MRQ-10
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Cheng L, et al. J Pathol. 2007; 211:1-9
References 2:
Weissferdt A, et al. Hum Pathol. 2015; 46:376-83
References 3:
Browne P, et al. Am J Clin Pathol. 2003 Nov;120(5):767-77
References 4:
García-Cosío M, et al. Mod Pathol. 2004 Dec;17(12):1531-8
References 5:
Gibson SE, et al. Am J Clin Pathol. 2006 Dec;126(6):916-24
Oct-3/4 is a member of the POU homeodomain family of transcription factors, which is expressed by embryonic stem cells and germ cells. A critical amount of Oct-3/4 is required to maintain stem cell self replication. Down regulation of Oct-3/4 levels are associated with loss of pluripotency. Oct-3/4 has been proposed as a useful marker for germ cell tumors which exhibit features of pluripotentiality, including seminoma/dysgerminoma/germinoma and embryonal carcinoma, and establishing a germ cell origin for some metastatic tumors of uncertain primary origin.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
N1NK
Concentration:
Greater than or equal to 69 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Del Sordo R et al. Histology and histopathology. 2014; 29(1):101-106
References 2:
Miettinen M et al. American Journal of Surgical Pathology. 2014; 38(3):410-420
References 3:
Paine SML et al. Neuropathology and applied neurobiology. 2014; 40:544-550
References 4:
Antic T et al. American Journal of Pathology. 2011; 136:872-880
Transcription factors containing the POU homeo domain have been shown to be important regulators of tissue-specific gene expression in lymphoid and pituitary differentiation and in early mammalian development. POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. Oct-3 (also known as Oct-4) is a mammalian POU transcription factor expressed by early embryo cells and germ cells. Oct-3/4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo. A critical amount of Oct-3/4 is required to sustain stem-cell self renewal, and up or down regulation induce divergent developmental programs. Two isoforms of Oct-3, termed Oct-3A and Oct-3B, are generated by alternative splicing. The gene which encodes Oct-3/4 maps to human chromosome 6p21.3. Oct-3/4 (C-10) is recommended for detection of Oct-3A (Oct-4) and Oct-3B of mouse, rat and human origin by Western Blotting, immunoprecipitation, immunofluorescence, and paraffin immunohistochemistry. Pre-treatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0, or in 50 mM Tris buffer pH9.5, for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based detection is recommended. Control tissue Seminoma or embryonal carcinoma. Staining Nuclear
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
C-10
Concentration:
n/a
Format:
Purified
Storage buffer:
Purified antibody in PBS with 0.2 % BSA and 15mM sodium azide
Storage:
2-8°C
References 1:
Drocourt, L., et al. 2002, J. Biol. Chem. 277: 25125-25132.
References 2:
Fong, Y.W., et al. 2011, Cell 147: 120-131.
References 3:
Wang, J., et al. 2011, Cancer Res. 71: 7238-7349.
References 4:
Rijlaarsdam, M.A., et al. 2011, Br. J. Cancer 105: 854-863.
Oct-2 is a transcription factor of the POU homeo-domain family that binds to the Ig gene octamer sites, regulating B-cell-specific genes. These are involved in proliferation and differentiation and, despite the scarce evidence for Oct-2 expression in T cells, it has been shown that this factor participates in transcriptional regulation during T-cell activation. Oct-2 activity is dependent on phosphorylation and alternatitive splicing.Various lymphomas are also positive for this marker including the following: Bchronic lymphocytic leukemia, mantle cell lymphoma, follicular lymphoma, marginal zone lymphoma, plasmacytoma, Burkitt lymphoma, diffuse large cell lymphoma, diffuse large B-cell lymphoma, T-cell rich B-cell lymphoma, nodular lymphocyte predominant Hodgkin lymphoma, and classic Hodgkin lymphoma.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN Ready To Use
Clone:
MRQ-2
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Browne P, et al. Am J Clin Pathol. 2003; 120:767-77
References 2:
García-Cosío M, et al. Mod Pathol. 2004; 17:1531-8
References 3:
Gibson SE, et al. Am J Clin Pathol. 2006; 126:916-24
EBS-C-002 reacts with NuMA or Nuclear Mitotic Apparatus protein, which at the onset of mitosis redistributes from the nucleus to two centrosomal structures at the poles of the mitotic spindle, where it plays a vital role in establishing and maintaining its bipolar structure. After anaphase the protein redistributes from the spindle polar region into the reforming nucleus and concentrates initially at the site where nuclear lamins and perichomatin have been reported to assemble. In contrast to mitotic cells, post-mitotic neurons display NuMA both in the nucleus and in the cytoplasm. Due to release from dead cells, NuMA is also used as oncological marker in serum and urine. In addition, chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 has been detected in patients with acute promyelocytic leukemia.
This antibody reacts with the C-terminus of Nucleophosmin (B23). NPM1 (Nucleophosmin 1, B23, nutramin, NO38) is a ubiquitously expressed phosphoprotein involved in ribosome assembly/transport, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity, centrosome duplication, and regulation of p53. NPM1 continuously shuttles between the nucleus, cytoplasm, nucleolus and chaperoning core histones from the nucleus to the cytoplasm.
UACA (Uveal Autoantigen with Coiled-coil domains and Ankyrin repeats) is a 1,416 amino acid nuclear membrane protein. It was originally identified as an autoantigen in patients with panuveitis, a characteristic of Vogt-Koyanagi-Harada disease, and in patients with Graves' disease. UACA was also later identified as Nucling, a mRNA differentially expressed in F9 embryonal carcinoma cells, and that is up-regulated during cardiac muscle differentiation. UACA appears to function as a pro-apoptotic protein that recruits the apaf-1- pro-caspase-9 complex for the induction of apoptosis to mediate the cell-death pathway.
Antibody Isotype:
IgG1,kappa
Monosan Range:
MONOSAN
Clone:
AE-5
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Yamada, K., et al. Biochem. Biophys. Res. Commun. 280: 1169-1176 (2001)
NTAL (non-T cell activation linker), also known as LAB (linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
NAP-07
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
NTAL (non-T cell activation linker), also known as LAB (linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
NAP-03
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
In lung cancer diagnosis Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is sofar not found in Human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non-SCLC (cell cultures).RNL-4 reacts with peripheral nerves and ganglia of various tissues and cross-reacts with smooth muscle cells and myoepithelium. In the central nervous system it reacts with the neurohypophysis and pars intermedia of the pituitary gland, and a weak diffuse staining was observed in neurons of the granular and molecular layer of the cerebellar cortex, while glial cells, cerebellar medulla and Purkinje cells are negative. Reticulon-1 has been found to indicate neuronal differentiation and to be downregulated in neurological pathologies.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
RNL-4
Concentration:
1 mg/ml
Storage buffer:
PBS with 0.09% sodium azide
Storage:
2-8°C
References 1:
Senden et al. Eur J Cell Biol 1996;69:197-213
References 2:
Senden et al. J Pathol 1997;182:13-21
References 3:
Hens et al. Cell Tissue Res 1998;292:229-237
References 4:
Kim et al. Biochem Biophys Res Comm 2000;276:329-334
In lung cancer diagnosis Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is sofar not found in Human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non-SCLC (cell cultures).RNL-2 recognizes an epitope located within the region of amino acids 421-589 of the neuro-endocrine specific protein Reticulon-1A (NSP-A), which is also present in the N-terminal part of Reticulon-1B (NSP-B). In normal tissues, RNL-2 reacts with brain Purkinje cells, pancreatic islet cells, cells in the pituitary gland and some (peripheral) nerve fibers. In addition, a few epithelia show positive staining.
In lung cancer diagnosis Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is sofar not found in Human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non-SCLC (cell cultures).RNL-2 recognizes an epitope located within the region of amino acids 421-589 of the neuro-endocrine specific protein Reticulon-1A (NSP-A), which is also present in the N-terminal part of Reticulon-1B (NSP-B). In normal tissues, RNL-2 reacts with brain Purkinje cells, pancreatic islet cells, cells in the pituitary gland and some (peripheral) nerve fibers. In addition, a few epithelia show positive staining.
NR2F6 (nuclear receptor subfamily 2 group F member 6), also known as EAR2 or ERBAL2, is a transcription factor involved in modulation of hormonal responses. NR2F6 represses e.g. transcription of the oxytocin-neurophysin gene, renin gene, lutropin-choriogonadotropic hormone receptor gene, and the thyroid hormone receptor gene. In the immune system, NR2F6 affects IL-17 expression in the Th-17 cells, thus also the balance between immunological tolerance and autoimmunity.SpecificityThe mouse monoclonal antibody OPAL1-01 recognizes an intracellular epitope of OPAL1 (outcome predictor in acute leukemia 1), a transmembrane adaptor protein expressed mainly by megacaryocytes and platelets.Application detailsWestern blotting: Positive control: HEK293T/17/NR2F6/Ha-tag transfectants, negative control: HEK293T/17 cells. <br>Flow cytometry: Recommended dilution: 3-9 µg/ml. Intracellular staining.
Antibody Isotype:
IgG2a kappa
Monosan Range:
MONOSAN
Clone:
EM-51
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Notch 1 is a 270-300 kDa transmembrane heterodimeric protein with multiple extracellular growth factor-like repeats, and with an intracellular domain consisting of multiple different domain types. It serves as a receptor for membrane ligands, such as Delta 1, Jagged 1 (CD339), and Jagged 2, and regulates cell fate decisions. Upon ligand binding the transmembrane form of Notch 1 is repeatedly cleaved to provide approximately 120 kDa Notch intracellular fragment (NICD), which translocates to the nucleus and acts as a part of transcriptional complexes that alter differentiation, proliferation, and apoptosis. The highest level of Notch 1 expression is in brain, lung and thymus.SpecificityThe mouse monoclonal antibody EM-51 recognizes NR2F6, a transcriptional repressor (intracellular antigen) expressed mainly in the heart, placenta, liver, skeletal muscle, kidney and pancreas, but also e.g. in T cell subpopulations.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml. Intracellular staining.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
mN1A
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
The monoclonal antibody HM.11 recognizes modified amino acid nitrotyrosine in all different species. Nitrotyrosine is formed in tissues in presence of the active metabolite NO and is a stable end product of nitrosylation of tyrosine. Inflammation is characterized by increased nitric oxide (NO) production. NO reacts rapidly with superoxide to form peroxynitrite. At physiological pH and in the presence of transition metals, peroxynitrite undergoes heterolytic cleavage to form hydroxyl anion and nitronium ion, the latter of which nitrates protein tyrosine residues. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques, Amyotrophic Lateral Sclerosis (ALS) and Multiple Sclerosis (MS). Thus, the presence of nitrotyrosine on proteins can be used as a marker for peroxynitrite formation in vivo and consequently as a marker of NO-mediated tissue damage. The monoclonal antibody HM.11 recognizes nitrotyrosine, both with the free amino acid as well as with proteins containing nitrotyrosine
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
HM11
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Ter Steege; J et al. Free Radic Biol Med 1998; 25: 953
References 2:
Casoni, F et al J Biol Chem 2005, 280: 16295
References 3:
Han; F et al. Resuscitation 2008; 79: 301
References 4:
Tsuhako H et al. Free radic Biol Med 2010; 48: 704
References 5:
Brunelli L et al. Metabolic brain disease 2012; 27:37
NHERF1 (Na+/H+ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50) is an adaptor protein, which associates with beta-catenin and is required for its localization at the cell-cell junctions, interacts with various G protein-coupled receptors and regulates their traffic, as well as sodium-hydrogen exchange and sodium-dependent phosphate transport. NHERF1/EBP50 inhibits cell motility and is required to suppress anchorage-independent growth. It contains C-terminal ERM (ezrin, radixin, moesin)-binding region and two N-terminal PDZ (postsynaptic-density-95/disc-large/ZO1 homology) domains and is able to form head-to-tail intramolecular conformation to regulate its interactions.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
EBP-10
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Nerve growth factor receptor (NGFR), also known as p75NTR, is a 75-kDa glycoprotein member of the tumor necrosis factor (TNF) receptor family essential for embryonic development of the peripheral nervous system. In normal tissue, NGFR is expressed in neural crest derived cells, lymphoid follicular dendritic cells seen in lymph nodes and tonsils, and myoepithelial cells of the breast, prostate, and salivary gland as well as basal epithelium of the respiratory system. NGFR has been shown to be a reliable adjunct marker for melanoma, specifically desmoplastic and spindle cell variants Anti-NGFR labels myoepithelial cells of the breast and may aid in the differentiation between benign conditions, pre-invasive neoplastic lesions and invasive malignancies of the breast.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN Ready To Use
Clone:
MRQ-21
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Thompson SJ. Am J Clin Pathol. 1989; 92:415-23
References 2:
Reis-Filho JS, et al. Mod Pathol. 2006; 19:307-19
References 3:
Lazova R, et al. J Am Acad Dermatol. 2010; 63:852-8
NG2 / chondroitin sulfate proteoglycan 4 is expressed on glial cell populations, but not on normal hepatopoietic cells. It is an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells, where it plays role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes, and supports signaling pathways important for tumor invasion and growth. NG2 also serves as an AML blast tumor marker associated with poor prognosis.SpecificityThe mouse monoclonal antibody mN1A recognizes intracellular domain of Notch 1 protein, mainly its activated form. The unprocessed Notch 1 protein is recognized with lower affinity.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
7.1
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs in most vertebrates are composed of three different polypeptide chains with different molecular weights – neurofilament heavy protein (NF-H), medium (NF-M) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. The protein segment on the C-terminal side of the human NF-H rod is uniquely long (more than 600 amino acids) compared to other IF proteins and is highly charged (> 24 % Glu, > 25 % Lys), rich in proline (> 12 %) and improverished in cysteine, methionine and aromatic amino acids. Its most remarkable feature is a repetitive sequence that covers more than half its lenght and includes the sekvence motif Lys-Ser-Pro (KSP) greater than 40 times. Plasma neurofilament heavy chain level has been proposed as a marker of axonal injury and clinical use of its degeneration and loss has been suggested as a biomarker of several neurodegenerative diseases.SpecificityThe mouse monoclonal antibody 7.1 recognizes an extracellular epitope of NG2, the melanoma-associated chondroitin sulfate proteoglycan 4 of Mw approximately 220-300 kDa.Application detailsELISA: Capture antibody. <br>Western blotting: Recommended dilution: 1-2 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
NF-05
Concentration:
1 mg/ml
Format:
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
The neurofilament (NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. RNF403 reacts exclusively with the phosphorylated isoform of the160 kD neurofilament protein.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
RNF403
Concentration:
1 mg/ml
Storage buffer:
PBS with 0.09% sodium azide
Storage:
2-8°C
References 1:
Bauwens et al. Ann Otol Rhinol Laryngol 1992;101:479-486
Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs in most vertebrates are composed of three different polypeptide chains with different molecular weights – neurofilament heavy protein (NF-H), medium (NF-M) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. The protein segment on the C-terminal side of the human NF-H rod is uniquely long (more than 600 amino acids) compared to other IF proteins and is highly charged (> 24 % Glu, > 25 % Lys), rich in proline (> 12 %) and improverished in cysteine, methionine and aromatic amino acids. Its most remarkable feature is a repetitive sequence that covers more than half its lenght and includes the sekvence motif Lys-Ser-Pro (KSP) greater than 40 times. Plasma neurofilament heavy chain level has been proposed as a marker of axonal injury and clinical use of its degeneration and loss has been suggested as a biomarker of several neurodegenerative diseases.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
NF-01
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Marker for the differentiation of 160 kD neurofilament peptide from other intermediate filament proteins. The antibody is useful for studying neurofilament expression and for tumor characterization (pheochromocytoma, paraganglioma, ganglioneuroblastoma, ganglioneuroma and other tumors of neuronal origin). Positive control: human brain tissue
Marker for the differentiation of 200 kD neurofilament peptide from other intermediate filament proteins. The antibody is useful for studying neurofilament expression and for tumor characterization (pheochromo-cytoma, paraganglioma, ganglioneuroblastoma, gan-glioneuroma and other tumors of neuronal origin). Positive control: Human brain tissue.
The antibody does stain neurons in sections of brain and other tissues. On an immunoblot on a crude human brain extract the antibody will only stain the 70 kDa band of neurofilament (ref. 1)
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
2F11
Concentration:
15 ug/ml
Storage buffer:
Culture medium with 0.01M Sodium Azide
Storage:
2-8°C
References 1:
Muijen GNP van et al. (1984) Am J Pathol. 116, 363-369
References 2:
Klück P et al. (1984) Lancet (March 24th), 652-653
References 3:
Muijen GNP van et al. (1985) Hum Pathol 16, 590-595
References 4:
Banks-Schlegel SP et al. (1985) Cancer Res 406, 339
Neurofilaments (NF) are intermediate filaments that provide structural support to the neuronal cytoskeleton.1-3 They are heteropolymers composed of three NF subunits: NF-H, NF-M, and NF-L.2 Anti-NF labels neurons of the central and peripheral nervous system as well as neoplastic cells of neuronal origin.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN Ready To Use
Clone:
2F11
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Diepholder HM, et al. Cancer. 1991; 68:2192-201
References 2:
Lee M, et al. J. Cell Biol. 1993; 122:1337-50
References 3:
Trojanowski JQ, et al. Hum Pathol. 1984; 15:248-57
Neurofilaments constitute the main structural elements of neuronal axons and dendrites. Neurofilaments are composed of three major subunits referred to as the neurofilament triplet, with molecular weights of 68 kD, 160kD and 200 kD. Within tumors, only neoplastic cells of neural origin or those exhibiting neuronal differentiation, have been reported to express neurofilaments. NF-H (200 kD) polypeptide of human neurofilament. This antibody reacts with both phosphorylated and unphosphorylated forms of NF-H.
Mab RNL-1, directed against N-CAM (Neural Cell Adhesion Molecule), reacts with normal neural tissues and endocrine glands, such as pancreatic islet, pituitary gland and adrenal medulla. Expression was also found in Leydig cells of the testis, in the thyroid and in smooth-muscle cells of the small intestine, colon and bladder. The antibody is a valuable marker for the characterization of several neuroendocrine tumors. In immunoblotting (Western) the antibody is reactive with 3 main clusters of protein bands in the molecular weight region of 200 kD, 100 kD, and 25-27 kD. Positive control: Pancreatic islet cells (cell surface staining).
NCK1 (NCK alpha) is a cytoplasmic adaptor protein that plays a universal role in coordinating the signaling networks critical for organizing the actin cytoskeleton, cell movement, or axon guidance, connecting transmembrane receptors to multiple intracellular signaling pathways. It contains one SH2 domain, through which NCK1 binds to phosphorylated domains of transmembrane signaling moleculs or certain adaptor proteins, and three SH3 domains for binding proline-rich sequences of other molecules involved in the process of nucleation and polymerization of the actin cytoskeleton.SpecificityThe antibody NF-05 recognizes a nonphosphorylated epitope of neurofilament heavy protein (NF-H), a 210 kDa intracellular structural protein of Intermediate Filament Proteins family. NF-H is mainly expressed in the central and peripheral nervous system and reproductive system and is biochemically very stable.Application detailsWestern blotting: Recommended dilution: 2 ?g/ml; positive control: Jurkat cell lysate. <br>Immunocytochemistry: Recommended dilution: 2 ?g/ml; positive control: human NCK1-transfected COS-7 cells.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
EM-06
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Napsin A has a specific function in normal alveolar epithelium and is proposed to play a role in the proteolytic processing of surfactant precursors. Napsin A is reported to be predominantly expressed in lamellar bodies of type II pneumocytes, secondary lysosomes of alveolar macrophages, respiratory epithelium of terminal and respiratory bronchioles, plasma cells, within a subset of lymphocytes in normal lung, as well as in epithelial cells of renal tubules in normal kidney and is weakly expressed in normal spleen. Studies have reported that Napsin A is expressed in 90% of primary lung adenocarcinomas.
Antibody Isotype:
IgG2b
Monosan Range:
MONXtra
Clone:
IP64
Concentration:
Greater than or equal to 8.3 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Yamashita Y et al. Modern Pathology. 2015; 28: 111-11
References 2:
Kandalaft PL et al. American Journal of Clinical Pathology. 2014; 142: 830-836
Napsin is a pepsin-like aspartic proteinase in the A1 clan of the AA clade of proteinases. There are two closely related napsins, napsin A (NAPSA) and napsin B (NAPSB). Napsin A is involved in processing propeptide pulmonary surfactant protein B (proSP-B) in the lung. In normal tissue, Napsin A is expressed in type II pneumocytes of the lung and proximal tubules of the kidney. Napsin A is a useful marker for lung adenocarcinoma.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN
Clone:
MRQ-60
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Jagirdar J. Arch Pathol Lab Med.; 132:384-96 (2008)
References 2:
Bishop JA, et al.. Hum Pathol.; 41:20-5 (2010)
References 3:
Ye J, et al. Appl Immunohistochem Mol Morphol.; 19:313-17 (2011)
References 4:
Mukhopadhyay S, et al. Am J Surg Pathol.; 35:15-25 (2011)
References 5:
Rawlings ND and Salvesen GS. Academic Press.; p.69-71 (2013)
Napsin is a pepsin-like aspartic proteinase in the A1 clan of the AA clade of proteinases. There are two closely related napsins, napsin A (NAPSA) and napsin B (NAPSB). Napsin A is involved in processing propeptide pulmonary surfactant protein B (proSP-B) in the lung. In normal tissue, Napsin A is expressed in type II pneumocytes of the lung and proximal tubules of the kidney. Napsin A is a useful marker for lung adenocarcinoma.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN Ready To Use
Clone:
MRQ-60
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Jagirdar J. Arch Pathol Lab Med.; 132:384-96 (2008)
References 2:
Bishop JA, et al.. Hum Pathol.; 41:20-5 (2010)
References 3:
Ye J, et al. Appl Immunohistochem Mol Morphol.; 19:313-17 (2011)
References 4:
Mukhopadhyay S, et al. Am J Surg Pathol.; 35:15-25 (2011)
References 5:
Rawlings ND and Salvesen GS. Academic Press.; p.69-71 (2013)
Reconstitute with 1 mL or 0.1 mL of sterile distilled water as indicated on vial label. The myotilin gene on chromosome 5q31 encodes a 498 amino acid polypeptide with a molecular weight of 57kD. Myotilin is a structural protein of sarcomeric Z discs and sarcolemma in human skeletal and cardiac muscle. It is homologous to palladin and titin in the two C-terminal lg-domains and also to palladin in its unique serine-rich N-terminal region. Myotilin interacts with alpha-actinin, actin and gamma-filamin. Mutations in the myotilin gene are associated with limb-girdle muscular dystrophy 1 A (LGMD1A) and one form of Myofibrillar Myopathy. It is highly conserved between human and mouse with its expression being more widespread in the embryo than in the adult. Expression of myotilin has been reported in adult skeletal and cardiac muscle with variable expression reported in the peripheral nervous system, lung, liver and kidney. NCL-MYOTILIN will be of use in studies to determine the expression of myotilin in normal and pathological tissues.
Antibody Isotype:
IgG1, kappa
Monosan Range:
MONXtra
Clone:
RSO34
Concentration:
n/a
Storage buffer:
Lyophilized
Storage:
2-8°C
References 1:
Mologni L et al. Mechanisms of Development. 103: 121125 (2001)
References 2:
Mykkänen OM et al. Mol. Biol. Cell. 12 (10): 30603073 (2001)
References 3:
Hauser MA et al. Human Molecular Genetics. 9 (14): 21412147 (2000)
References 4:
van der Ven PFM et al. Journal of Cell Biology. 151 (2): 235247 (2000)
References 5:
Salmikangas P et al. Human Molecular Genetics. 8 (7): 13291336 (1999
Myosin is a contractile muscle specific protein composed of two heavy and four light chains. The myosin heavy chain has many isoforms which are specific for different muscles or fiber types, some of which are developmentally regulated. The range of myosin heavy chain antibodies may prove useful for investigating development of intrafusal and extrafusal muscle fibers and the course of muscle fiber regeneration. At the ultrastructural level, antibodies can reveal architectural details of the myofilament as well as the cytoplasmic and membrane sites of new myosin integration. The user is required to reconstitute the contents of the vial with the correct volume of sterile distilled water as indicated on the vial label. Rabbit myosin slow type heavy chain. Crossreacts with human myosin slow type heavy chain.The antibody also reacts with type I myosin heavy chain in rat, mouse, dog, sheep, pig and goat muscle.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
WB-MHCS
Concentration:
n/a
Storage buffer:
Lyophilized tissue culture supernatant containing 15 mM sodium azide.
Storage:
2-8°C
References 1:
Sheriffs IN et al. Journal of Clinical Pathology. 54: 517520 (2001)
References 2:
Ecob-Prince M et al. Journal of Neurological Sciences. 91: 7178 (1989)
References 3:
Carson NE et al. The Journal of Histotechnology. 21 (1): 1924 (1998)
References 4:
Ecob-Prince M et al. Journal of Neurological Sciences. 90: 167177 (1989)
References 5:
Vivarelli E et al. Journal of Cellular Biology. 107: 21912197 (1988)
Myosin is a contractile muscle specific protein composed of two heavy and four light chains. The myosin heavy chain has many isoforms which are specific for different muscles or fiber types, some of which are developmentally regulated. The range of myosin heavy chain antibodies may prove useful for investigating development of intrafusal and extrafusal muscle fibers and the course of muscle fiber regeneration. At the ultrastructural level, antibodies can reveal architectural details of the myofilament as well as the cytoplasmic and membrane sites of new myosin integration. The user is required to reconstitute the contents of the vial with the correct volume of sterile distilled water as indicated on the vial label. Rabbit myosin fast type heavy chain. Crossreacts with human myosin fast type heavy chain. Rabbit myosin neonatal type heavy chain. Crossreacts with human myosin neonatal type heavy chain. Note that this antibody recognises a myosin heavy chain present during the neonatal period in rabbit limb muscle. The temporal appearance of an equivalent epitope may differ in different species and consequently it may not be correct to label the epitope as neonatal in some circumstances.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
WB-MHCN
Concentration:
n/a
Storage buffer:
Lyophilized tissue culture supernatant containing 15 mM sodium azide.
Storage:
2-8°C
References 1:
Ecob-Prince M et al. Journal of Neurological Sciences. 90: 167177 (1989)
References 2:
Ecob-Prince M et al. Journal of Neurological Sciences. 91: 7178 (1989)
Myosin is a contractile muscle specific protein composed of two heavy and four light chains. The myosin heavy chain has many isoforms which are specific for different muscles or fiber types, some of which are developmentally regulated. The range of myosin heavy chain antibodies may prove useful for investigating development of intrafusal and extrafusal muscle fibers and the course of muscle fiber regeneration. At the ultrastructural level, antibodies can reveal architectural details of the myofilament as well as the cytoplasmic and membrane sites of new myosin integration. The user is required to reconstitute the contents of the vial with the correct volume of sterile distilled water as indicated on the vial label. Rabbit myosin fast type heavy chain. Crossreacts with human myosin fast type heavy chain. The antibody also reacts with type II myosin heavy chain (both IIa and IIb) in rat, mouse, dog, sheep, pig and goat muscle.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
WB-MHCf
Concentration:
n/a
Storage buffer:
Lyophilized tissue culture supernatant containing 15 mM sodium azide.
Storage:
2-8°C
References 1:
Sheriffs IN et al. Journal of Clinical Pathology. 54: 517520 (2001)
References 2:
Ecob-Prince M et al. Journal of Neurological Sciences. 91: 7178 (1989)
References 3:
Carson NE et al. The Journal of Histotechnology. 21 (1): 1924 (1998)
References 4:
Hoh JFY and Hughes A. Journal of Muscle Research and Cellular Motility. 10: 312325 (1989)
Myosin is a contractile muscle specific protein composed of two heavy and four light chains. The myosin heavy chain has many isoforms which are specific for different muscles or fiber types, some of which are developmentally regulated. The range of myosin heavy chain antibodies may prove useful for investigating development of intrafusal and extrafusal muscle fibers and the course of muscle fiber regeneration. At the ultrastructural level, antibodies can reveal architectural details of the myofilament as well as the cytoplasmic and membrane sites of new myosin integration. The user is required to reconstitute the contents of the vial with the correct volume of sterile distilled water as indicated on the vial label
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
RNMy2/9D2
Concentration:
n/a
Storage buffer:
Lyophilized tissue culture supernatant containing 15 mM sodium azide.
Storage:
2-8°C
References 1:
Davis CE et al. Neuromuscular Disorders. 1 (6): 411421 (1991)
References 2:
Ecob-Prince M et al. Journal of Neurological Sciences. 91: 7178 (1989)
Mab 414 reacts with myosin-containing elements in most cell types. Antigen localization: cytoplasm In Western blot experiments the antibody reacts with a 75kD part of the heavy chain (epitope location on a myosin-common site-chain), no reaction with light chains. <br>It is not reactive with alpha-cardiac myosin. Positive control: muscle, myofibrils (chicken)
Rhabdomyosarcomas are a class of myoblast-derived soft tissue sarcomas that usually express a number of muscle-specific genes and primarily affect children and young adults. Differentiation of myogenic cells is controlled by a set of regulatory genes including MyoD1, myogenin, Myf-5 and Myf-6. Myf-4 is the human homolog of myogenin. Its gene product, together with that of Myf-3, accumulates in the nucleus of differentiated cells.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
LO26
Concentration:
Greater than or equal to 13 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Den Bakker MA et al. Histopathology. 2003; 43(3):297299
References 2:
Ingeholm P et al. APMIS. 2002; 110(9):639645
References 3:
Kumar S et al. Modern Pathology. 2000; 13(9):988993
References 4:
Gilpin BJ et al. Journal of Biological Chemistry. 1998; 273(1):157166
Myogenin also identified as myogenic factor 4 is a muscle specific transcription factor associated with muscle differentiation and cell cycle.1 Anti-myogenin reactivity is seen in the nuclei of myoblasts in developing muscle tissue. Anti-myogenin is a useful immunohistochemical reagent for identification of rhabdomyosarcoma.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN Ready To Use
Clone:
F5D
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Miller JB. J Cell Biol.; 111:1149-59 (1990)
References 2:
Wang NP, et al. Am J Pathol.; 147:1799-810 (1995)
References 3:
Cui S, et al. Pathol Int.; 49:62-8 (1999)
References 4:
Kaspar et al. Sci Rep.; 5:15090 (2015)
References 5:
Rudzinski et al. Am J Surg Pathol.; 38:654-9 (2014)
Recognizes a phosphor-protein of 45kDa, identified as MyoD1. The epitope of this MAb maps between amino acid 180-189 in the C-terminal of mouse MyoD1 protein. It does not cross react with Myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilms tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and in rhabdomyosarcomas, neuroblastomas, Ewings sarcomas and alveolar soft part sarcomas. Pre-treatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0, or in 50 mM Tris buffer pH9.5, for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based detection is recommended. Control tissue Rhabdomyosarcoma. Staining Nuclear (only nuclear staining should be considered as evidence of skeletal muscle differentiation).
Antibody Isotype:
IgG1, kappa
Monosan Range:
MONOSAN
Clone:
5.8A
Concentration:
n/a
Format:
Concentrate
Storage buffer:
Bioreactor Concentrate with 0.05% Azide
Storage:
2-8°C
References 1:
Thulasi R et. al. Cell Growth and Differentiation, 1996, 7(4):531-41.
References 2:
Wesche WA et. al. American Journal of Surgical Pathology, 1995, 19(3):261-9.
This antibody is specific to a 45 kDa protein, which is identified as MyoD1. This antibody is specific to an epitope of amino acid 3-56 in the N-terminus of mouse MyoD1. This antibody does not react with myogenin, Myf5 or Myf6. MyoD1 stains the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue but is expressed strongly in the tumor cell nuclei of rhabdomyosarcomas.
BM-5 is specific marker for human myeloid cells and an early marker of myeloid differentiation. It recognizes a nuclear and cytoplasmic antigen present in granulocytes, monocytes, and myeloid precursor cells. It also reacts with a subset of myeloid leukemia cells. BM-5 has no reactivity with any other cell type in human tissues.
BM-4 recognizes a nuclear antigen expressed in human granulocytes (83%) monocytes (20%) and myeloid precursor cells residing in lymphoid and non-lymphoid tissues. BM-4 is an early marker of myeloid differentiation. It also reacts with a subset of myeloid leukemia cells. BM-4 has no reactivity with any other cell type in human tissues.
Induction studies using HL-60 cells showed that BM-3 identifies a nuclear antigen which is expressed during the early phases of myeloid differentiation, making BM-3 an early marker of myeloid differentiation. It is found in 98% of human granulocytes, in 80% of human monocytes and in myeloid precursor cells, residing in lymphoid and non-lymphoid tissues. It also reacts with a subset of myeloid leukemia cells. BM-3 has no reactivity with any other cell type in human tissues. In experiments using S-35 methionine labeled human myeloid leukemia cells BM-3 immunoprecipitated a 13 kDa protein.
Until recently, immunological markers for myeloid cells have been lacking, especially those which identify different levels of cellular differentiation. The BM series provides a new panel of monoclonal antibodies which stain early precursor and mature forms of human myeloid cells. This panel of monoclonal antibodies reacts with antigenic determinants present in normal myeloid cells and leukemias of similar derivation. BM-2 recognizes a cytoplasmic antigen expressed in mature human granulocytes (polys) residing in lymphoid and non-lymphoid tissues. It does not react with any other cell type in human tissues.
Until recently, immunological markers for myeloid cells have been lacking, especially those which identify different levels of cellular differentiation. The BM series provides a new panel of monoclonal antibodies which stain early precursor and mature forms of human myeloid cells. This panel of monoclonal antibodies reacts with antigenic determinants present in normal myeloid cells and leukemias of similar derivation. BM-2 recognizes a cytoplasmic antigen expressed in mature human granulocytes (polys) residing in lymphoid and non-lymphoid tissues. It does not react with any other cell type in human tissues.
BM-1 is reactive in B5 fixed, paraffin embedded tissue sections to human myeloid cells and derived malignancies. The antibody reacts with a 183 kDa cytoplasmic antigen with DNAbinding characteristics which is expressed in most myeloid precursor cells and myeloid leukemias. BM-1 is positive on myeloid precursors in bone marrow but lymph nodes are negative. Tissue granulocytes are positive as well as scattered cells in peripheral cortex and interlobular septae of adult and fetal thymus. Portal regions of fetal liver (18 weeks) are also positive
The monoclonal antibody 8F4 recognizes mouse myeloperoxidase (MPO). MPO is a glycoprotein produced as a single precursor, which is subsequently cleaved into a alpha and beta chain. In human the biologically active MPO is a 150kDa tetramer composed of 2 glycosylated alfa chains of 59-64 kDa and 2 beta chains of 14 kDa. MPO is stored in azurophilic granules of polymorphonuclear leukocytes and is rapidly released into the phagosome and extracellular space during inflammatory conditions.The enzyme catalyzes the conversion of chloride and hydrogen peroxide to hypochlorite, a potent oxidant, which functions in host defense against microorganisms. </br> Involvement of MPO has been described in several human diseases, such as cardiovascular disease, airway inflammation, lung cancer, Alzheimer's disease and multiple sclerosis. A positive correlation between elevated MPO levels in serum and cardiovascular disease suggest an interesting role for MPO as an diagnostic marker, making it possible to identify patients at risk for future cardiac events. Furthermore, there are some autoimmune diseases, in which MPO is targeted by antineutrophil cytoplasm antibodies. Studies with MPO-knockout mice have shown an increased susceptibility to pneumonia following intratracheal infections. Moreover, MPO deficient mice are more susceptible to experimental autoimmune encephalitis, a T cell-dependent neuronal disease, and have an increased expression of arteriosclerotic plaques compared to wild-type mice.</br> The anti-mouse MPO monoclonal antibody 8F4 recognizes natural MPO in biological solutions by ELISA, in frozen tissue sections fixed with acetone and in flow cytometry using a cell permeabilization method.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
8F4
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Huugen; D et al. Am J Pathol 2005; 167: 47
References 2:
Wang, D et al Blood 2006, 108:1223
References 3:
Matthijsen; M et al. Am J Pathol 2007; 171: 1743
References 4:
Leeuwen van M et al. Arterioscler Thromb Vasc Biol 2008; 28: 84
Mouse anti Human myeloperoxidase antibody, clone 2C7 recognizes human myeloperoxidase (MPO). MPO is an important component of azurophilic granules in neutrophils, being involved in microbicidal processes. The protein is a multimer of 2 heavy chains (55 kDa) and two light chains (15 kDa), the heavy chains being linked by a disulphide bond. Mouse anti Human Myeloperoxidase antibody, clone 2C7 recognizes native MPO in Western blots, and the heavy chain following boiling of the sample. Mouse anti Human Myeloperoxidase antibody, clone 2C7 also recognizes recombinant MPO in western blots and weakly in ELISA.Mouse anti Human myeloperoxidase antibody, clone 2C7 may be of value in the study of myeloid cells and myeloid leukaemias by flow cytometry following cell permeabilization. Mouse anti Human myeloperoxidase antibody, clone 2C7 did not recognize rat MPO by ELISA (Patry et al. 2003).
The monoclonal antibody 266-6K1 recognizes human myeloperoxidase (MPO), an ~135 glycoprotein expressed in all cells of the myeloid linage. MPO functions as an ?2β2 heteromultimer consisting of two heavy (?) and two light (β) chains of 55 and 15 kDa respectively. MPO is abundantly present in azurophilic granules of polymorphonuclear neutrophils (PMNs). It is an important enzyme used during phagocytic lysis of engulfed foreign particles which takes part in the defense of the organism through production of hypochlorous acid (HOCl), a potent oxidant. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. Upon activation of neutrophils, MPO can be rapidly released and as such useful in body fluids as marker for inflammatory status. Involvement of MPO has been described in numerous diseases such as atherosclerosis, lung cancer, Alzheimer's disease, inflammatory bowel disease and multiple sclerosis. Autoimmune antibodies to MPO (so called ANCA) are involved in Wegenerâs disease. Since the discovery of MPO deficiency, initially regarded as rare and restricted to patients suffering from severe infections, MPO has attracted more clinical attention.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
266-6K1
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
La Rocca; G et al. Basic Res Cardiol 2009; 104: 307
In paraffin sections antibody pm 43 recognizes only myelin in the peripheral nervous system (PNS). In frozen sections it also reacts with myelin in the central nervous system (CNS). Is useful for studying myelination and demyelination processes in the PNS, and remyelination processes in the CNS as can be observed after trauma in multiple sclerosis. Reacts with a 43 kD protein in immunoblotting. Positive control: Peripheral nerve.
1032 Is specific for the major vault protein, a 104-kDa highly conserved protein interacting with estrogen receptor. It is one of a series of four mAbs which recognize different epitopes of the protein. Major vault proteins have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for >70% of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
1032
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Abbondanza, C. et al, J. Cell Biol. 141, 1301-1310 (1998)
1027 Is specific for the major vault protein, a 104-kDa highly conserved protein interacting with estrogen receptor. It is one of a series of four mAbs which recognize different epitopes of the protein. Major vault proteins have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for >70% of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
1027
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Abbondanza, C. et al, J. Cell Biol. 141, 1301-1310 (1998)
1014 Is specific for the major vault protein, a 104-kDa highly conserved protein interacting with estrogen receptor. It is one of a series of four mAbs which recognize different epitopes of the protein. Major vault proteins have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for >70% of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
1014
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Abbondanza, C. et al, J. Cell Biol. 141, 1301-1310 (1998)
Muscle specific actin (MSA) is a highly conserved, ubiquitous protein found in muscle and some non-muscle cells. Actins can be divided into three subsets, alpha actins found in muscle tissue cells, beta and gamma actins found in non-muscle cells and a small subset of gamma actins also found in muscle tissue cells. In normal tissues, expression is found in striated fibers of skeletal muscle, smooth muscle in arteries, veins and pericytes of smaller arteries, muscle in bowel, myometrium of the uterus, prostatic stroma, capsule cells of liver, kidney, lymph node and spleen, the myoepithelial layers of mammary ducts and glands, eccrine sweat glands and salivary glands. Expression is not found in epithelial cells, lymphoid cells, macrophages, connective tissue and neuronal cells. Human muscle specific alpha- and gamma-actin isomers. Reactive with alpha-actin from skeletal, cardiac and smooth muscle sources. Does not react with non-muscle actin, beta or non-smooth muscle gamma-actin isomers. Crossreacts with porcine, bovine, monkey, rabbit, hamster and rat muscle actin.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
SC28
Concentration:
Greater than or equal to 13 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Tsutsumi Y et al. Pathology International. 1995; 45(2):10
References 2:
Nicolas MM et al. Human Pathology 2010;41:663-671
References 3:
Guillou L. Diagnostic Histopathology 2008;14:527- 535.
The antibody labels a 50kDa, multiple myeloma oncogen-1 (MUM1) protein. MUM1 is encoded by the MUM1/IRF-4 gene, which is mapped to 6q23-25 and identified as a myeloma-associated oncogene. It is a member of the interferon regulatory factor family of transcription factors and plays an important role in the regulation of gene expression in response to signaling by interferon and other cytokines. MUM1 positive cells express the protein in the nucleus in a diffuse and microgranular pattern. However, some positivity is also observed in the cytoplasm of MUM1-expressing cells. In normal/reactive lymphoid tissues, such as lymph node, this antibody stains plasma cells, some B-cells in the light zone of germinal centers, and a subset of T-cells (T-cells in germinal centers and interfollicular areas). MUM1 expression has been described in diffuse large B-cell lymphoma (DLBCL). MON 3318 can stain other Bcell lymphomas such as lymphoplasmacytic lymphoma, chronic lymphocytic leukemia, follicular lymphoma, marginal zone lymphoma, lymphoblastic lymphoma/leukemia, primary effusion lymphoma, DLBCL, Burkitt-like lymphoma, and classical Hodgkin lymphoma.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN Ready To Use
Clone:
MRQ-8
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Falini B, et al. Blood. 2000; 95:2084-92
References 2:
Grossman A, et al. Genomics. 1996; 37:229-33
References 3:
Neresh KN. Haematologica. 2007; 92:267-8
References 4:
Van Imhoff GW, et al. J Clin Oncol. 2006; 24:4135-42
References 5:
Gualco G, et al. Appl Immunohistochem Mol Morphol. 2010; 18:301-10
The MUM-1 (multiple myeloma oncogene 1) gene was originally identified because of its involvement in the t(6:14) translocation observed in multiple myeloma, which causes the juxtaposition of the MUM-1 gene to the Ig heavy chain locus. MUM-1 is expressed in late plasma cell directed stages of B cell differentiation and in activated T cells, suggesting that MUM-1 may serve as a marker for lympho-hemopoietic neoplasms derived from these cells. The morphologic spectrum of MUM-1 expressing cells has been found to range from that of a centrocyte to that of a plasmablast/plasma cell. Consequently the histogenic value of MUM-1 may be to provide a marker to aid in the identification of the transition from BCL-6 positive (germinal center B cells) to CD138 positive (immunoblasts and plasma cells).
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
EAU32
Concentration:
Greater than or equal to 263 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Bergsagel P and Kuehl W.Oncogene. 2001: 20(40);5611-5622
Clones AE1 and AE3 are specific for the 56.5, 50, 50', 48 and 40 kD acidic cytokeratins as well as the 65 to 67, 64, 59, 58, 56 and 52 kD basic cytokeratins. The cocktail of clones AE1 and AE3 exhibit broad reactivity with two families of cytokeratin, acidic and basic.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
AE1/AE3
Concentration:
Greater than or equal to 225 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Nadji M, Morales AR. Laboratory Medicine. 1983; 14:767
References 2:
Omata M et al. Am.J.of Clin. Pathol. 1980; 73:626
References 3:
Su T et al. Diagnostic Pathology. 2014; 9: 179
References 4:
Zhao W et al. Int.J. of Clin. and Exp.Pathol. 2014; 7(11): 7951-7956
References 5:
Hammers HJ et al. Molecular Cancer Therapeutics. 2010; 9(6): 1525-1535
The antibody reacts with mucin from small and large intestine and to a weaker extent with salivary and breast epithelia. Stomach, pancreas, kidney, and ovary epithelia are negative. Positive reaction of gastric cancer and colon cancer (antibody shows a relative high specificity for colon carcinoma). Reactivity on cultured cell lines: LS 174 T (colon cancer cell line). Positive control: Small intestine.
The human Mucin-1 antibody is reactive with 90% of breast carcinoma, most epithelial ovarian carcinoma and a high portion of the lung, urogenital and gastrointestinal carcinomas. Some reactivity is observed in the apical membrane of normal breast epithelium. Weak reactivity is also present in pancreas, kidney and lung. BC-2 reacts with a five amino acid epitope of the MUC-1 core protein which is less affective for glysosilation than most other MUC-1 epitopes. Localization: cytoplasm and membrane. Positive control: Mamma carcinoma.
Mucins are high molecular weight glycoproteins which constitute the major component of the mucus layer that protects the gastric epithelium from chemical and mechanical aggressions. In humans, at least 14 mucin genes have been identified that code for the mucin proteins. MUC6 is a secretory mucin that is part of a family of at least 14 high molecular weight glycoproteins made by many epithelial tissues. MUC6 is preferentially expressed in non-neoplastic gastric tissue, specifically in the pyloric glands. During neoplastic transformation, mucin expression may be altered within these tissues leading to particular patterns of expression.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN Ready To Use
Clone:
MRQ-20
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Ruco LP, et al., Am J Clin Pathol. 92:273-9 (1989)
References 2:
Facchetti F, et al., Histopathology. 19:141-5 (1991)
References 3:
Do SI. et al. J of Breast Cancer. 16:152-8 (2013)
References 4:
Vergier B et al. Blood., 9597: 2212-8 (2000)
References 5:
Mino-Kenudson M, et al. Virchows Arch. 469:255-65 (2016)
9-13M1 recognizes the peptide core of gastric mucin M1/MUC5AC), and more specifically with the d epitope amongst the a, b, c, d, e, f, g and h protein core epitopes defined by Bara for M1. 9-13M1 and 2-11M1 react exclusively with epitopes located in the the Nterminal cysteine-rich part of the peptide core MUC5AC. MUC5AC is present in primary ovarian mucinous cancer and gastric cancer, but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification pancreatic carcinoma and pre-cancerous changes vs. normal pancreas
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
9-13M1
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bara, J. et al., Cancer Res.46: 3983-3989 (1986)
References 2:
Bara, J. et al., Biochem. J. 254: 185-193 (1988)
References 3:
Bara, J. et al., Int. J. Cancer 47: 304-310 (1991)
References 4:
Bara, J. et al., J. Immunol. Methods 149: 105-113 (1992)
References 5:
Guyonnet Duperat V. et al., Biochem. J. 305: 211 219 (1995)
58M1 recognizes the peptide core of gastric mucin M1 (now: MUC5AC), and more specifically with the e epitope amongst the a, b, c, d, e, f, g and h protein core epitopes defined by Bara for M1. MUC5AC is present in primary ovarian mucinous cancer and gastric cancer, but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
58M1
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bara, J. et al., Cancer Res.46: 3983-3989 (1986)
References 2:
Bara, J. et al., Biochem. J. 254: 185-193 (1988)
References 3:
Bara, J. et al., Int. J. Cancer 47: 304-310 (1991)
References 4:
Bara, J. et al., J. Immunol. Methods 149: 105-113 (1992)
References 5:
Guyonnet Duperat V. et al., Biochem. J. 305: 211 219 (1995)
45M1 recognizes the peptide core of gastric mucin M1 (now: MUC5AC), and more specifically with the h epitope amongst the a, b, c, d, e, f, g and h protein core epitopes defined by Bara for M1. 45M1 and 2-12M1 both specifically react with epitopes located in the C-terminal cysteine rich part of the peptide core of MUC5AC. MUC5AC is present in primary ovarian mucinous cancer and gastric cancer, but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
45M1
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bara, J. et al., Int. J. Cancer 47: 304-310 (1991)
References 2:
Bara, J. et al., J. Immunol. Methods 149: 105-113 (1992)
2-12M1 recognizes the peptide core of gastric mucin M1/MUC5AC, and more specifically with the c epitope amongst the a, b, c, d, e, f, g, and h protein core epitopes defined by Bara for M1. 2-12M1 and 45M1 both specifically react with epitopes located in the Cterminal cysteine rich part of the peptide core of gastric mucin (MUC5AC). MUC5AC is present in primary ovarian mucinous cancer and gastric cancer, but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. AntiMUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
2-12M1
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bara, J. et al., Cancer Res.46: 3983-3989 (1986)
References 2:
Bara, J. et al., Biochem. J. 254: 185-193 (1988)
References 3:
Bara, J. et al., Int. J. Cancer 47: 304-310 (1991)
References 4:
Bara, J. et al., J. Immunol. Methods 149: 105-113 (1992)
References 5:
Guyonnet Duperat V. et al., Biochem. J. 305: 211 219 (1995)
2-11M1 recognizes the peptide core of gastric mucin M1/MUC5AC, and more specifically with the b epitope amongst the a, b, c, d, e, f, g, and h protein core epitopes defined by Bara for M1. 2-11M1 and 9-13M1 react exclusively with epitopes located in the N-terminal cysteine-rich part of the peptide core MUC5AC. MUC5AC is present in primary ovarian mucinous cancer and gastric cancer, but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
2-11M1
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bara, J. et al., Cancer Res.46: 3983-3989 (1986)
References 2:
Bara, J. et al., Biochem. J. 254: 185-193 (1988)
References 3:
Bara, J. et al., Int. J. Cancer 47: 304-310 (1991)
References 4:
Bara, J. et al., J. Immunol. Methods 149: 105-113 (1992)
References 5:
Guyonnet Duperat V. et al., Biochem. J. 305: 211 219 (1995)
1-13M1 recognizes the peptide core of gastric mucin M1/MUC5AC), and more specifically with the a epitope, which is the most abundant amongst the a, b, c, d, e, f, and h protein core epitopes defined by Bara for M1. MUC5AC is present in primary ovarian mucinous cancer and gastric cancer, but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification pancreatic carcinoma and precancerous changes vs. normal pancreas.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
1-13M1
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bara, J. et al., Cancer Res.46: 3983-3989 (1986)
References 2:
Bara, J. et al., Biochem. J. 254: 185-193 (1988)
References 3:
Bara, J. et al., Int. J. Cancer 47: 304-310 (1991)
References 4:
Bara, J. et al., J. Immunol. Methods 149: 105-113 (1992)
References 5:
Guyonnet Duperat V. et al., Biochem. J. 305: 211 219 (1995)
MUC4 (mucin 4) is a large membrane-anchored glycoprotein of the mucin family that is expressed by epithelial cells in various normal tissues including lung, bronchus, stomach, colon, and cervix. MUC4 is generally not detected in normal pancreas, but is expressed in the vast majority of pancreatic neoplasms, such as pancreatic ductal adenocarcinoma. Additionally, expression in various carcinomas has been reported, including gastric adenocarcinoma, colon adenocarcinoma, and lung adenocarcinoma.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN
Clone:
8G7
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Moniaux N, et al. J Histochem Cytochem. 2004; 52:253-61
MUC4 (mucin 4) is a large membrane-anchored glycoprotein of the mucin family that is expressed by epithelial cells in various normal tissues including lung, bronchus, stomach, colon, and cervix. MUC4 is generally not detected in normal pancreas, but is expressed in the vast majority of pancreatic neoplasms, such as pancreatic ductal adenocarcinoma. Additionally, expression in various carcinomas has been reported, including gastric adenocarcinoma, colon adenocarcinoma, and lung adenocarcinoma.
EBS-T-232 reacts with SITTTE in the VNTR domain of human MUC3. The mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats (VNTRs) encoded by 60 base pairs (MUC1), 69 base pairs (MC2) and 51 base pairs (MUC3). Cancer cells of colon, breast and stomach, normal cells of salivary gland, breast, lung, and gastrointestinal tract are positive for MUC3.
Antibody Isotype:
IgG2b-K
Monosan Range:
MONOSAN
Clone:
EBS-T-232
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Apostolopoulos, V. et al. J. Gastroenterol. Hepatol. 1995; 10 (5): 555-561
Mucins are high molecular weight glycoproteins which constitute the major component of the mucus layer that protects the gastric epithelium from chemical and mechanical injury. In humans, at least 14 mucin genes have been identified that code for the mucin proteins. Reportedly, mucin expression is associated with tumor type of gastric carcinomas, with MUC2 being associated with mucinous carcinomas. Anti- MUC2 reactivity is seen in goblet cells of the small intestine and colon, and it is useful in immunohistochemistry for identifying colonic, gastric and esophageal carcinomas.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN Ready To Use
Clone:
MRQ-18
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Chaves P, et al. Dis Esophagus. 2005; 18:383-7
References 2:
Leteurtre E, et al. World J Gastroenterol. 2006; 12:3324-31.
References 3:
Mino-Kenudson M, et al. Arch Pathol Lab Med. 2007; 131:86-90
References 4:
Mizoshita T, et al. Histol Histopathol. 2007; 22:251-60
References 5:
OConnell FP, et al. Arch Pathol Lab Med. 2005; 129:338-47
VU-4H5 reacts with the protein core of MUC1, an apical cell side epithelial marker which is upregulated or switched on in the majority of carcinomas. The dominant epitope of VU-4H5 is PDTR, located in the VNTR domain of MUC1. In tissue sections, VU-4H5 also displays prominent staining of the cytoplasm.
VU-3D1 reacts with the protein core of MUC1, an apical cell side epithelial marker which is upregulated or switched on in the majority of carcinomas. The dominant epitope of VU3D1 is SAPDTRPA, located in the VNTR domain of MUC1.
VU-3C6 reacts with the protein core of MUC1, an apical cell side epithelial marker which is upregulated or switched on in the majority of carcinomas. The dominant epitope of VU-3- C6 is GVTSAPDTRPAP, located in the VNTR domain of MUC1. Binding of VU-3C6 is enhanced after glycosylation of the DTR motif.
VU-2G7 reacts with the protein core of MUC1, an apical cell side epithelial marker which is upregulated or switched on in the majority of carcinomas. The dominant epitope of VU2G7 includes the PDTR motif, located in the VNTR domain of MUC1. Binding of VU-2G7 is significantly enhanced when the threonine of the PDTR motif bears a GalNAc
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
2G7
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Ryuko, K. et al. Tumor Biol. 21(4): 197-210 (2000)
References 2:
Karsten, U. et al. Cancer. Res. 58(12): 2541-2549 (1998)
VU-13F11 reacts with the protein core of MUC1, an apical cell side epithelial marker which is upregulated or switched on in the majority of carcinomas. The dominant epitope of VU-13F11 has not yet been determined but is located in the VNTR domain of MUC1 (confirmed by ELISA).
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
VU-13F11
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Schol D. et al, Tumor Biol. 19(Suppl 1): 35-45 (1998)
VU-12E1 reacts with the protein core of MUC1, an apical cell side epithelial marker which is upregulated or switched on in the majority of carcinomas. The dominant epitope of VU-12- E1 is PDTRPAP, located in the VNTR domain of MUC1. Binding of VU-12E1 is enhanced after glycosylation of the DTR motif
VU-11E2 reacts with the protein core of MUC1, an apical cell side epithelial marker which is upregulated or switched on in the majority of carcinomas. The dominant epitope of VU11E2 is TSAPDTRP, located in the VNTR domain of MUC1. Binding of VU-11E2 is enhanced after glycosylation of the DTR motif
VU-11D1 reacts with the protein core of MUC1, an apical cell side epithelial marker which is upregulated or switched on in the majority of carcinomas. The dominant epitope of VU11D1 is TSAPDTRP, located in the VNTR domain of MUC1. Binding of VU-11D1 is enhanced after glycosylation of the DTR motif.
Mucins are high molecular weight glycoproteins which constitute the major component of the mucus layer that protects the gastric epithelium from chemical and mechanical injury. In humans, at least 14 mucin genes have been identified that code for the mucin proteins. Mucin genes are expressed in a regulated cell- and tissue-specific manner. The stomach provides a good example of such differential expression of mucin genes. MUC1 is detected in mucous cells of the surface epithelium and neck region of the gastric antrum, as well as in pyloric glands and oxyntic glands of the body region. The heterogeneous pattern of mucin expression, including the expression of the intestinal mucin MUC2, may provide new insights into the differentiation pathways of gastric carcinoma.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN Ready To Use
Clone:
MRQ-17
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Chaves P, et al. Dis Esophagus. 2005; 18:383-7
References 2:
Leteurtre E, et al. World J Gastroenterol. 2006; 12:3324-31.
References 3:
Mino-Kenudson M, et al. Arch Pathol Lab Med. 2007; 131:86-90
References 4:
Mizoshita T, et al. Histol Histopathol. 2007; 22:251-60
References 5:
OConnell FP, et al. Arch Pathol Lab Med. 2005; 129:338-47
MSH6 is a mismatch repair gene which is deficient in a high proportion of patients with microsatellite instability (MSI-H). This finding is associated with the autosomal dominant condition known as Hereditary Non-Polyposis Colon Cancer (HNPCC). The anti-MSH6 antibody is useful in screening patients and families for this condition. Colon cancers that are microsatellite unstable have a better prognosis than their microsatellite stable counterparts.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN Ready To Use
Clone:
44
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Lagerstedt Robinson K, et al. J Natl Cancer Inst. 2007 Feb 21;99(4): 291-9
References 2:
Niessen RC et al. Gut 2006 Dec;55(12):1781-8
References 3:
Lawes DA et al. Br J Cancer. 2005 Aug 22; 93(4):472-7
References 4:
Stormorken AT et al. J Clin Oncol. 2005 Jul 20;23(21):4705-12
References 5:
Rigau V et al. Arch Pathol Lab Med. 2003;127:694-700
MSH2 is a mismatch repair gene which is deficient in a high proportion of patients with microsatellite instability (MSI-H). This finding is associated with the autosomal dominant condition known as Hereditary Non-Polyposis Colon Cancer (HNPCC). The anti-MSH2 antibody is useful in screening patients and families for this condition. Colon cancers that are microsatellite unstable have a better prognosis than their microsatellite stable counterparts.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
G219-1129
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Christensen M et al. Cancer 2002;95: 2422-30
References 2:
Wright CL et al. Am J Surg Pathol. 2003;27: 1393-1406
References 3:
Renkonen E et al. J Clin Oncol 2003; 21: 3629-3637
References 4:
Hoedema R. et al. The American Surgeon 2003, May 69(5): 387-92
References 5:
Brueckl WM et al. Anticancer Research 2003; 23: 1773-1778
MSH2 is a mismatch repair gene which is deficient in a high proportion of patients with microsatellite instability (MSI-H). This finding is associated with the autosomal dominant condition known as Hereditary Non-Polyposis Colon Cancer (HNPCC). The anti-MSH2 antibody is useful in screening patients and families for this condition. Colon cancers that are microsatellite unstable have a better prognosis than their microsatellite stable counterparts.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN Ready To Use
Clone:
G219-1129
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Christensen M et al. Cancer 2002;95: 2422-30
References 2:
Wright CL et al. Am J Surg Pathol. 2003;27: 1393-1406
References 3:
Renkonen E et al. J Clin Oncol 2003; 21: 3629-3637
References 4:
Hoedema R. et al. The American Surgeon 2003, May 69(5): 387-92
References 5:
Brueckl WM et al. Anticancer Research 2003; 23: 1773-1778
The antibody reacts with an internal epitope of MRP3, a 190-200 kD transmembrane protein that is closely related to the multidrug resistance protein MRP1. M3II-21 was raised against a bacterial fusion protein of MRP3, containing amino acids 830-949 of the protein. M3II-21 does not cross-react with the human MDR1, MRP1, MRP2 or MRP5 gene products. M3II-21 has potential value for detection of MRP3-mediated drug-resistance in human tumor samples.
M3II-9 reacts with an internal epitope of MRP3, a 190-200 kD transmembrane protein that is closely related to the multidrug resistance protein MRP1. M3II-9 was raised against a bacterial fusion protein of MRP3, containing amino acids 830-949 of the protein. M3II-9 does not cross-react with the human MDR1, MRP1, MRP2 or MRP5 gene products
M2III-6 reacts with an internal epitope of cMOAT/MRP2, a 170-180 kD transmembrane protein known as the canalicular multi-organic anion transporter, absent in patients with the Dubin-Johnson syndrome, an autosomal recessive liver disorder characterized by chronic conjugated hyperbilirubinemia. cMOAT/MRP2 is closely related to the multidrug resistance related protein MRP, and cMOAT/MRP2 overexpression has been observed in a subset of cisplatin resistant cell lines. M2III-6 was raised against a bacterial fusion protein of cMOAB/MRP2, containing the 202-amino acid COOH terminal end of the protein. M2III-6 did not cross react with the human MDR1, MRP1, MRP3 and MRP5 gene products.
The antibody reacts with an internal epitope of MRP2, a 190-200 kD transmembrane protein earlier known as the canalicular multi-organic anion transporter cMOAT, absent in patients with the Dubin-Johnson syndrome, an autosomal recessive liver disorder characterized by chronic conjugated hyperbilirubinemia. MRP2 is a member of the MRP family of multidrug resistance related proteins, and MRP2 overexpression has been observed in a subset of cisplatin resistant cell lines. M2III-5 was raised against a fusion protein of the bacterial maltose binding protein and rat Mrp2, containing the 202-amino acid COOH terminal end of the transporter protein. The Mab detects rat, mouse and human MRP2. M2III-5 does not cross-react with the human MDR1 P-gp, MRP1, MRP3 orMRP5 gene products.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
M2III-5
Concentration:
250 ug/ ml
Storage buffer:
cell culture supernatant with 0.7% BSA and 0.1% sodium azide
M2II-12 reacts with an internal epitope of cMOAT/MRP2, a 190-200 kD transmembrane protein known as the canalicular multi-organic anion transporter, absent in patients with the Dubin-Johnson syndrome, an autosomal recessive liver disorder characterized by chronic conjugated hyperbilirubinemia. cMOAT/MRP2 is closely related to the multidrug resistance related protein MRP, and cMOAT/MRP2 overexpression has been observed in a subset of cisplatin resistant cell lines. M2II-12 was raised against a bacterial fusion protein of cMOAB/M¬RP2, containing amino acids 860-950 of the protein. M2II-12 did not cross react with the human MDR1, MRP1, MRP3 and MRP5 gene products.
MRPm6 reacts with an internal epitope of MRP, a 180-195 kD transmembrane transporter protein overexpressed in various human non-P-glycoprotein MDR tumor cell lines. MRPm6 was raised against a bacterial fusion protein of MRP, containing a segment of 170 amino acids in the carboxy terminal end and part of the carboxy proximal nucleotide binding domain of the protein. MRPm6 does not crossreact with the human MDR1 and MDR3 gene products
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MRPm6
Concentration:
250 ug/ ml
Storage buffer:
Serum free tissue culture supernatant with 0.7% BSA and 0.1% sodium azide
Storage:
2-8°C
References 1:
Moll I et al. Eur J Cell Biol 2005; 84: 259-271
References 2:
Riedel I et al. Virchows Arch 2001; 438: 181-191
References 3:
Romih R et al. Cell Biol 1998; 109: 263-269
References 4:
Demirkesen C et al. J Cutan Pathol. 1995; 22: 518-535
The antibody reacts with an internal epitope of MRP1, a 180-195 kD transmembrane transporter protein overexpressed in various human non-P-glycoprotein MDR tumor cell lines. MRPm5 was raised against a bacterial fusion protein of MRP1, containing amino acids 986-1204 of the protein. MRPm5 does not cross-react with the human MDR1 and MDR3 gene products.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
MRPm5
Concentration:
100 ug/ ml
Format:
Protein G purified
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Cole S et al. Science 1992; 258: 1650-1654
References 2:
Flens M et al. Cancer Res 1994; 54: 4557-4563
References 3:
Zaman et al. Proc Nat Acad Sci 1994; 91: 8822-8826
47-8D3 reacts with macrophages and detects the well-known leukocyte L1, cystic fibrosis antigen. Detecting a single protein band of 14 kDa in Western blots of lysates of human monocytes and granulocytes, the antigen was identified as the calcium-binding protein MRP14, which is a member of the S100 family involved a.o. in regulating the cell cycle. MRP14 is also implicated in the abnormal differentiation of myeloid cells in the stroma of cancer. It is further found on squamous mucosal epithelia. When associated with MRP8 it forms the heterodimer calprotectin.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
47-8D3
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Flavell DJ. et al., J. Histochem. Cytochem. 35: 1217-1226 (1987)
References 2:
Facchetti F. et al., Am. J. Clin. Pathol. 92: 42-50 (1989)
References 3:
Bardadin KA. et al., J. Pathol. 164: 253-259 (1991)
References 4:
Goebeler M. et al., J. Leukocyte Biol. 55: 259-261 (1994)
47-8D3 reacts with macrophages and detects the well-known leukocyte L1, cystic fibrosis antigen. Detecting a single protein band of 14 kDa in Western blots of lysates of human monocytes and granulocytes, the antigen was identified as the calcium-binding protein MRP14, which is a member of the S100 family involved a.o. in regulating the cell cycle. MRP14 is also implicated in the abnormal differentiation of myeloid cells in the stroma of cancer. It is further found on squamous mucosal epithelia. When associated with MRP8 it forms the heterodimer calprotectin.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
47-8D3
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Flavell DJ. et al., J. Histochem. Cytochem. 35: 1217-1226 (1987)
References 2:
Facchetti F. et al., Am. J. Clin. Pathol. 92: 42-50 (1989)
References 3:
Bardadin KA. et al., J. Pathol. 164: 253-259 (1991)
References 4:
Goebeler M. et al., J. Leukocyte Biol. 55: 259-261 (1994)
The monoclonal antibody V1q recognizes mouse tumor necrosis factor alpha (TNF-?). TNF-? is the prototype cytokine of the family of TNF-related ligands, which are based on structural and functional homologies. TNF-? is synthesized as type II transmembrane protein. TNF-? can be recognized by two different membrane receptors, namely TNF-R1 and TNF-R2. TNF-? is present in a membrane-bound (tmTNF) as well as soluble form (sTNF). The membrane-bound form of TNF-? is recognized by both TNF receptors with high affinity, whereas the soluble form is recognized more superiorly by TNF-R1. TNF-? is produced by many different cell types including macrophages, T lymphocytes, NK cells, neutrophils and endothelial cells. Cells differ in the expression of the two TNF-receptors and sTNF versus tmTNF, respectively. TNF-?, a homotrimeric 17 kDa protein, is a potent mediator of inflammatory and metabolic functions. TNF-? was originally detected as a highly cytotoxic cytokine for tumor cells, it causes tumor necrosis in vivo and shows cytolytic activity against tumor cells in vitro. Furthermore, TNF-? has been implied as central mediator in shock induced by gram negative micro-organisms. TNF-? induces on its turn the production of many other cytokines. Furthermore, TNF-? has been found in inflammatory foci such as synovial effusions in rheumatoid arthritis, systemic circulation in septic shock, parasitemia and rejection of renal transplants. The monoclonal antibody V1q recognizes both natural and recombinant TNF-? and shows neutralizing activity.
Monosan Range:
MONOSAN
Clone:
V1q
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Echtenacher; B et al. J Immunol 1990; 145: 3762
References 2:
Gerspach, J et al Microsc Res Tech 2000, 50: 243
References 3:
Demjen; D et al. Nat Med 2004; 10: 389
References 4:
Rajashekhar G et al. Physiol Genomics 2007; 31: 104
The antibody reacts with mouse EGF in ELISA (10 ng detectable) and in spot blots (1 ng detectable). In immunohistochemistry the antibody reacts with mouse salivary glands. No crossreaction with rat EGF.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
E5
Concentration:
5 ug/ml
Storage buffer:
Culture medium with 0.01M Sodium Azide
Storage:
2-8°C
References 1:
Beerstecher HJ et al. (1988) J Histochem Cytohistochem 36, 1153-1160
This monoclonal antibody binds to human MMP-9. Reactivity in other species has not been determined. The 12-mer peptide used for the immunization differs on 4 or 5 amino acids with the sequence of MMP-9 of rat, mouse, and rabbit. Therefore it is not expected to be effective in these species as well. The antibody was tested for cross-reactivity with MMP-1, MMP-2, and MMP-3 and did not cross-react.
The antibody binds to human MMP-3. Reactivity in other species has not been determined. The antibody was tested for cross-reactivity with MMP-1, MMP-2 and MMP-9 and did not cross-react.
MLH1 is a mismatch repair gene that is deficient in a high proportion of patients with microsatellite instability (MSI-H). This finding is associated with the autosomal dominant condition known as Hereditary Non-Polyposis Colon Cancer (HNPCC). The anti-MLH1 antibody is useful in screening patients and families for this condition. Colon cancers that are microsatellite unstable have a better prognosis than their microsatellite stable counterparts
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN Ready To Use
Clone:
G168-728
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Christensen M et al. Cancer 2002;95: 2422-30
References 2:
Wright CL et al. Am J Surg Pathol. 2003;27: 1393-1406
References 3:
Renkonen E et al. J Clin Oncol 2003; 21: 3629-3637
References 4:
Hoedema R. et al. The American Surgeon 2003, May 69(5): 387-92
References 5:
Brueckl WM et al. Anticancer Research 2003; 23: 1773-1778
Mi is a transcription factor implicated in pigmentation, bone development and in mast cells. Various forms of Mi exist ranging from 50-70 kD in size. This antibody targets the 52-56 kD range. This antibody has been useful in identifying malignant melanoma.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN Ready To Use
Clone:
C5/D5
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Liegl B, et al. Am J Surg Pathol. 2008; 32:608-14
References 2:
Righi A, et al. Int J Surg Pathol. 2008; 16:16-20
References 3:
Weinreb I, et al.. Virchows Arch. 2007; 450:463-70
References 4:
Ohsie SJ, et al. J Cutan Pathol. 2008; 35:433-44
References 5:
Hornick JL, et al. Am J Surg Pathol. 2008; 32:493-501
Mismatch repair gene Postmeiotic segregation Increased 2, also known as PMS1 homolog 2, is a ubiquitous gene encoding the mismatch repair protein (MMR) PMS1 protein homolog 2 (PMS2). PMS2 functions by repairing mutations occurring during DNA replication, in normal proliferating cells.
Antibody Isotype:
IgG
Monosan Range:
MONXtra
Clone:
M0R4G
Concentration:
Greater than or equal to 520 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Silva F et al. Sao Paulo Medical Journal. 2009? 127(1):46- 51
References 2:
Vos M et al. Biochemical Society Transactions. 2005? 33(4):718720
Mismatch repair gene MutS Homolog 2 is a ubiquitous gene encoding the mismatch repair protein (MMR) MutS protein homolog 2 (MSH2). MSH2 functions by repairing mutations occurring during DNA replication, in normal proliferating cells.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
79H11
Concentration:
n/a
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Jensen UB et al. Breast Cancer Research and Treatment. 2009; 120 (3): 777-782
Mismatch repair gene hMLH1 is a ubiquitous gene encoding the mismatch repair protein (MMR) MutL protein homolog 1 (MLH1). MLH1 functions by repairing mutations occurring during DNA replication, in normal proliferating cells.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
ES05
Concentration:
Greater than or equal to 165 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Tamura G et al.World Journal of Gastroenterology 2006; 12(2): 192198
References 2:
Abdel-Rahman W et al. Critical Reviews in Oncology/Hematology 2006; 58: 208220
References 3:
Mitchell R et al. American Journal of Epidemiology 2002; 156:885902
References 4:
Kuismanen S et al. American Journal of Pathology 2000; 156(5): 17731779
Mismatch repair gene MutS Homolog 6 is a ubiquitous gene encoding the mismatch repair protein (MMR) MutS protein homolog 6 (MSH6). MSH6 functions by repairing mutations occurring during DNA replication, in normal proliferating cells.
Antibody Isotype:
IgG
Monosan Range:
MONXtra
Clone:
PU29
Concentration:
Greater than or equal to 200 mg/L
Storage buffer:
Tissue culture supernatant with 15mM sodium azide
Storage:
2-8°C
References 1:
Warren J et al. Molecular Cell. 2007; 26:579-592
References 2:
Marti T et al. Journal of Cellular Physiology. 2002; 191:28-41.
MAP2a and 2b (270 kDa) being found mostly in dendrites, stabilize microtubules (shift the reaction kinetics in addition of new subunits and microtubule growth) and participate in determining the structure of different parts of vertebrate nerve cells.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MT-01
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Microphthalmia transcription factor (MITF) gene product, a nuclear transcription factor of the basic-helix-loop-helix type, is thought to play a role in the regulation of genes encoding the enzymes necessary for melanogenesis. These include tyrosinase, TRP-1 and TRP-2. MITF is critical for the embryonic development and postnatal viability of melanocytes. The melanocyte-specific isoform of microphthalmia transcription factor MITF-M, is reported to be expressed in normal and malignant melanocytes. The other isoforms, MITF-A, MITF-C and MITF-H, differ structurally at the N-terminus from MITF-M.
Antibody Isotype:
IgG1, kappa
Monosan Range:
MONXtra
Clone:
34CA5
Concentration:
n/a
Storage buffer:
Tissue culture supernatant with 15mM Sodium azide
Storage:
2-8°C
References 1:
Fang D and Setaluri V. Biochem. and Biophys. Research Comm. 256 (3): 657663 (1999)
References 2:
King R et al. American Journal of Pathology. 155 (3): 731738 (1999)
References 3:
Amae S et al. Biochem.and Biophys.Research Comm. 247: 710715 (1998)
References 4:
Watanabe A et al. Nature Genetics. 18: 283286 (1998)
MICA and MICB glycoproteins are members of MHC class I family, closely linked to HLA-B. However, unlike HLA molecules, MICA and MICB are not associated with beta2 microglobulin and are conformationally stable in the absence of conventional MHC class I peptide ligands. Both proteins are stress-induced antigens expressed mainly in gastrointestinal epithelium, where they are recognized by V-delta1 subset of gamma/delta T cells, and also on diverse epithelial tumor cells. Binding of MICA/MICB receptor, the NKG2D, leads to cytolytic response of NK cells, Tc cells, and gamma/delta T cells. Alternative splicing results in multiple isoforms, and some of them have been associated with susceptibility to psoriasis and psoriatic arthritis. Shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma.SpecificityThe mouse monoclonal antibody EM-06 recognizes NCK1 (NCK alpha), an ubiquitously expressed cytoplasmic SH2/SH3 adaptor protein important for organization of actin cytoskeleton structures.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
6D4
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
MHC class II molecules are encoded by polymorphic MHC genes and consist of a non-covalent complex of an ? and ? chain. Helper T lymphocytes bind antigenic peptides presented by MHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes.
Antibody Isotype:
IgG2-K
Monosan Range:
MONOSAN
Clone:
EBS-O-110
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Sparrow RL, et al., Transplantation 42: 647-652 (1986)
References 2:
Chorvath B et al. Neoplasma 34(4): 417-425 (1987)
References 3:
Horejsi V et al. Tissue Antigens 32(1): 6-11 (1988)
MHC class II molecules are encoded by polymorphic MHC genes and consist of a non-covalent complex of an ? and ? chain. Helper T lymphocytes bind antigenic peptides presented by MHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes.
Antibody Isotype:
IgG2b-K
Monosan Range:
MONOSAN
Clone:
LN-3
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Marder, R.L. et al. Lab. Invest. 52: 497-504 (1985)
References 2:
Andrade, R.E. et al. Human Pathology 19: 932-941 (1988)
References 3:
Azumi N. et al. Human Pathology 19: 1376-1382 (1988)
MHC class II molecules are encoded by polymorphic MHC genes and consist of a non-covalent complex of an ? and ? chain. Helper T lymphocytes bind antigenic peptides presented by MHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
Bra30
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Chorvath B et al. Neoplasma 34(4): 417-425 (1987)
References 2:
Horejsi V et al. Tissue Antigens 32(1): 6-11 (1988)
MHC class II molecules are encoded by polymorphic MHC genes and consist of a non-covalent complex of an ? and ? chain. Helper T lymphocytes bind antigenic peptides presented by MHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumulating in endosomal/lysosomal compartments and on the surface of B-cells, HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
EBS-O-111
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Thompson CJ et al., Human Immunol 6: 133-150 (1983)
EBS-O-114 reacts with HLA Class II. The antibody was shown to strongly block cytotoxic activity of T4+ cytotoxic T cell clones. Distribution studies on cell lines suggested that EBS-O-114 is directed against a DQ rather than a DR antigen.
MHC class II molecules are encoded by polymorphic MHC genes and consist of a non-covalent complex of an ? and ? chain. Helper T lymphocytes bind antigenic peptides presented by MHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes.
Antibody Isotype:
IgG3-K
Monosan Range:
MONOSAN
Clone:
Bra14
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Chorvath B et al. Neoplasma 34(4):417-425 (1987)
References 2:
Horejsi V et al. Tissue Antigens 32(1):6-11 (1988)
MHC class II molecules are encoded by polymorphic MHC genes and consist of a non-covalent complex of an ? and ? chain. Helper T lymphocytes bind antigenic peptides presented by MHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes.
Antibody Isotype:
IgG2b-K
Monosan Range:
MONOSAN
Clone:
BraFB6
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Babusikova O et al., Neoplasma 32(6): 657-62 (1985)
EP-4 recognizes the HLA-B27 cell surface antigen on human cells. HLA-B27 has been found to be highly associated (60%) with ankylosing spondylitis (Bekhterev's disease, MarieStrümpell disease or "bamboo spine"). While rheumatoid factor tests will be negative, testing for the presence of HLA-B27 in a patient can confirm the diagnosis of ankylosing spondylitis. Also postgonococcal arthritis and acute anterior uveitis are associated with HLA-B27.
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
EP-4
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Hansen JA et al. Hematol Oncol Clin North Am, 4(3): 507-515 (1990)
References 2:
El-Shabrawi, Y. et al. Ophthalmology 113: 695-700 (2006)
108-2C5 recognizes an intralocus determinant present on a limited number of HLA-A locusencoded gene products (HLA-A2, -A3, A28, -A29, -A30, -A31 and -Aw33). Furthermore, by testing its reactivity with HLA-A2 natural variants and mutants, the importance of amino acid residues 79 and/or 80 of the alpha1 domain was demonstrated in the formation of an intralocus HLA-A determinant.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
108-2C5
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Lozano, F. et al., Immunogenetics 30: 50-53 (1989)
References 2:
Lozano, F. et al., Tissue Antigens 35: 193-195 (1990)
References 3:
Domenech, N. et al., Human Immunol 30: 140-146 (1991)
References 4:
Ryschich, E, et al, Clin Cancer Res. 11(2 Pt 1): 498-504 (2005)
EBS-O-104 reacts with a monomorphic determinant of human major histocompatibility (MHC) class I antigens (HLA-A, B and C). Human MHC class I antigens are expressed constitutively on all nucleated cells lymphocytes such as lymphocytes, thymocytes, granulocytes, and bone marrow cells and also platelets but are absent on erythrocytes. MHC class I antigens play a role in class I MHCassociated antigen presentation, inhibition of NK cell cytotoxicity, tumor surveillance, and tissue allotransplantation.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
EBS-O-104
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Young NT et al. Hum Immunol 52(1): 1-11 (1997)
References 2:
Krensky AM et al, Transplant Proc 28(6): 3026-8 (1996)
References 3:
Hansen JA et al, Hematol Oncol Clin North Am 4(3): 507-515 (1990)
Bra23/9 reacts with a monomorphic determinant of human major histocompatibility (MHC) class I antigens (HLA-A, B and C). Human MHC class I antigens are expressed constitutively on all nucleated cells and platelets and are absent on erythrocytes. MHC class I antigens play a role in class I MHCassociated antigen presentation, inhibition of NK cell cytotoxicity, tumor surveillance, and tissue allotransplantation.
MFG-06 reacts with a glycoprotein of 40-45 kDa, known as Milk fat globule-EGF factor 8 protein (MFG-E8), lactadherin, p47 or milk fat globule 1 antigen. It is present on normal epithelial cells in various organs and considered a differentiation marker in carcinomas. It contains one EGF-like domain and 2 F5/8 type C domains. Functioning as a specific ligand for Integrin ?5 and Integrin ?3, MFG-E8 is thought to be involved in gamete interactions and cell attachment, possibly playing a role in fertilization and apoptosis. Additionally, MFG-E8 binds to rotavirus and inhibits its replication, thereby protecting the cell from viral infection. Overexpression of MFG-E8 is associated with breast cancer, suggesting that MFG-E8 may be related to tumorigenesis or progression. Among testicular carcinomas, seminomas are negative.
Recombinant prokaryotic fusion protein corresponding to approximately 100 amino acids which are present in the membrane-bound form of the mesothelin molecule.
Mesothelin is a glycosyl-phosphatidylinositol-linked (GPI) glycoprotein of 40kD present on the surface of mesothelial cells, mesotheliomas, epithelial ovarian cancers and some squamous cell carcinomas. It is synthesized as a 69 kD precursor which is enzymatically processed into an N-terminal secreted form of 30 kD and the GPI-linked membrane-bound form of 40 kD. The secreted form is identical to the megakaryocyte potentiating factor, but it is the GPI-linked membrane-bound form which has generated interest. Mesothelin is abundantly expressed in the kidney and in occasional epithelial cells of the trachea, tonsil and fallopian tube. The function of mesothelin is unclear but it may have a role in cellular adhesion. Mesothelin is reported to be abundant in the normal mesothelial cells from which malignant mesotheliomas and ovarian cystadenocarcinomas are derived.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
5B2
Concentration:
Greater than or equal to 40 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Ordonez NG. American Journal of Surgical Pathology. 2003; 27(11):14181428
References 2:
Ordonez NG. Modern Pathology. 2003; 16(3):192197
References 3:
Argani P et al. Clinical Cancer Research. 2001; 7(12):38623868
Hector Battifora mesothelial-1 (HBME-1) is a membrane antigen that exists in the microvilli of mesothelial cells and other epithelial cells. Anti-HBME-1 labels thyroid papillary carcinoma and follicular carcinoma but not normal thyroid making it a valuable marker for distinguishing thyroid malignacies from benign thyroid lesions. It has also been demonstrated to label mesothelial cells, both be
Antibody Isotype:
IgM
Monosan Range:
MONOSAN Ready To Use
Clone:
HBME-1
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Coli A, Bigotti G, et al. J Exp Clin Cancer Res. 2007 Jun;26(2):221-7
The muscle-specific form of laminin, merosin, is composed of three chains: alpha 2, beta 1 and gamma 1.Analyte Specific Reagent. Analytical and performance characteristics are not established. Reacts strongly with laminin alpha 2 chain of merosin in human and rabbit skeletal muscle. No reaction is observed in muscle sections from mouse, rat, dog, chicken, hamster or pig. The product is a lyophilized tissue culture supernatant containing sodium azide as a preservative. The user is required to reconstitute the contents of the vial with the correct volume of sterile distilled water as indicated on the vial label
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
Mer3/22B2
Concentration:
n/a
Storage buffer:
Lyophilized
Storage:
2-8°C
References 1:
Marafioti T et al. American Journal of Pathology. 162 (3): 861871 (2003
References 2:
Hess J et al. Molecular and Cellular Biology. 21 (5): 15311539 (2001)
References 3:
Re D et al. Cancer Research. 61 (5): 20802084 (2001)
References 4:
Luo Y and Roeder R G. Molecular and Cellular Biology. 15 (8): 41154124 (1995)
This antibody stains a minority of primary melanomas and half of the metastatic lesions tested. It rarely stains dysplastic naevi or common cellular naevi using standard immunohistochemical conditions. The antibody recognizes two protein bands in immunoblotting with a molecular weight of 95-100 kD.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
PAL-M2
Concentration:
10 ug/ml
Storage buffer:
Culture medium with 0.01M Sodium Azide
Storage:
2-8°C
References 1:
Ruiter DJ et al., (1985) J Invest Dermatol 85, 4-8
Antibody PAL-M1 stains the majority of primary melanomas and the large majority of metastases and is directed against the transferrin receptor (CD71). Local staining of dysplastic nevocellular nevi may be observed. Common nevocellular nevi rarely stain.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
PAL-M1
Concentration:
15 ug/ml
Storage buffer:
Culture medium with 0.01M Sodium Azide
Storage:
2-8°C
References 1:
Ruiter DJ et al., (1985) J Invest Dermatol 85, 4-8
References 2:
Muijen G van et al. (1990) J Invest Dermatol 95, 65-69
Anti-HMB45 is a useful melanoma immunohistochemical marker that reacts with antigens present on immature melanosomes. Anti-HMB45 is useful for identifying amelanotic melanoma from other neoplastic lesions with similar morphology.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN Ready To Use
Clone:
HMB-45
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Gown AM, et al. Am J Pathol. 1986; 123:195-203
References 2:
Wick MR, et al. Arch Pathol Lab Med. 1988; 112:616-20
References 3:
Abrahamsen HN, et al. Cancer. 2004; 100:1683-91
References 4:
Vaggelli L, et al. Tumori. 2000; 86:346-8
References 5:
Baisden BL, et al. Am J Surg Pathol. 2000; 24:1140-6
The antibody s useful as an immunohistochemical reagent to stain melanocytes and tumors derived therefrom. Anti-PNL2 reactivity is identified in the cytoplasm of cutaneous and oral mucosal melanocytes. Anti-PNL2 labels intraepidermal nevi, while the dermal components of compound nevi are largely non-reactive.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
PNL2
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
The antibody s useful as an immunohistochemical reagent to stain melanocytes and tumors derived therefrom. Anti-PNL2 reactivity is identified in the cytoplasm of cutaneous and oral mucosal melanocytes. Anti-PNL2 labels intraepidermal nevi, while the dermal components of compound nevi are largely non-reactive.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN Ready To Use
Clone:
PNL2
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
This antibody recognizes a molecular complex consisting of two bands with MW of 150 kD and 90 kD respectively (reduced 4 subunits 120 kD, 95 kD, 29 kD and 25 kD). The antibody reacts strongly with melanoma cells derived from cell lines and short term cultures and melanoma cells in frozen tissue sections. The antigen detected by this antibody is found to be associated with the adhesion, spreading and motility of human cultured melanoma cells. Cross reactions: The antibody reacts with a proportion of naevi and with endothelial cells of small vessels.
This antibody recognizes a high molecular weight proteoglycan with a molecular weight of > 450 kD (chondroitin sulfate) and 250 kD (core protein). The antibody reacts strongly with melanoma cells derived from cell lines and short term cultures and reacts preferentially with melanoma cells in frozen tissue sections. The antibody can also be used to detect melanoma lesions in vivo. Crossreactivity: The antibody reacts with most naevi and perineurium, and shows weak reactivity with hair follicles.
This antibody recognizes a heterogeneous 25-110 kD glycoprotein that is located mainly in the inner side of membranes of cytoplasmic vesicles in melanoma cells. The antigen has a 25 kD unglycosylated precursor in formalin fixed and paraffin-embedded tissue sections. Cross reactivity: The antibody reacts with some mucus producing tumors, carcinoids, carcinomas of the thyroid, mast cells, histiocytes in tumor regions and with cells with secretory functions such as salivary glands, bronchial glands, sweat glands, pancreas and prostate. The antibody should be used on formalin fixed paraffin embedded tissue sections, it is not advisable to use the antibody on frozen sections.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
NKI/C3
Concentration:
n/a
Storage buffer:
50 mM PBS at pH 7.4 with 1% BSA and 15mM sodium azide
Storage:
2-8°C
References 1:
MacKie, R.M., et al., J. Clin. Pathol. 37, 367 (1984)
References 2:
van Duinen, S.G., et al., Cancer 53, 1566 (1984)
References 3:
Vennegoor, C., et al., Int. J. Cancer 35, 287 (1985)
References 4:
Palmer, A.A., et al., Pathology 17, 335 (1985)
References 5:
Hagen, E.C., et al., Histopathology 10, 689 (1986)
Melanoma associated antigen (MAA) is dispersed in the cytoplasma of melanoma cells, and is more concentrated inside vacuoles and sometimes on the melanosomes. Occasionally the antigen is seen on the cell surface. The antigen is actively shed from living cells. Although the antigen is associated with melanomas, it is not codistributed with the tyrosinase activity associated with melagonesis. The antigen shows codistribution with cathepsin D, which is a marker for lysosomal functions. The antibody NKI/beteb recognizes a (pre)melanosomal 100 kD and 7 kD antigen (glycoprotein). The antibody reacts with melanomas, clear cells sarcomas (melanoma of soft tissue), nevocellular nevi, and normal melanocytes. Except for one case of non-Hodgkin's lymphoma in which macrophages were positive, no reactions with other tumors or tissues have been observed.
The antibody is a monoclonal, anti-melanoma antibody that reacts with an antigen that has yet to be identified.1 Notably used as a melanoma marker, KBA.62 also detects smooth muscle, basal cells of the epidermis and hair shaft epithelia of the skin.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
KBA.62
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Pages C, et al.. Hum Pathol. 2008; 39:1136-42
References 2:
Aung P, et al. Am J Surg Pathol. 2012; 36:265-72
References 3:
E Cohen-Knafo et al. J Clin Pathol. 1995; 48:826-831
References 4:
Kaufmann O, et al.. Mod Pathol, 1998 Aug; 11(8):740-6
The antibody is a monoclonal, anti-melanoma antibody that reacts with an antigen that has yet to be identified.1 Notably used as a melanoma marker, KBA.62 also detects smooth muscle, basal cells of the epidermis and hair shaft epithelia of the skin.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN Ready To Use
Clone:
KBA.62
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Pages C, et al.. Hum Pathol. 2008; 39:1136-42
References 2:
Aung P, et al. Am J Surg Pathol. 2012; 36:265-72
References 3:
E Cohen-Knafo et al. J Clin Pathol. 1995; 48:826-831
References 4:
Kaufmann O, et al.. Mod Pathol, 1998 Aug; 11(8):740-6
Melan A, a product of the MART-1 gene, is a melanocyte differentiation marker recognized by autologous cytotoxic T lymphocytes. Other melanoma-associated markers recognized by autologous cytotoxic T cells are reported to include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1 and GAGE-1. The analysis of these different molecules and their expression in individual melanomas may be of help in the study of their particular molecular roles in melanocyte differentiation and tumorigenesis.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
A103
Concentration:
Greater than or equal to 22 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Shidham VB et al. BioMed Central cancer. 2003; 3(1):15
References 2:
Clarkson KS et al. Journal of Clinical Pathology. 2001; 54:196200
References 3:
De Vries TJ et al. Journal of Pathology. 2001; 193:1320
References 4:
Fang D et al. American Journal of Pathology. 2001; 158(6):21072115
References 5:
Chen YT et al. Proceedings of the National Academy of Sciences USA. 1996; 93:59155919
M2I-4 reacts with an internal epitope of cMOAT/MRP2, a 170-180 kD transmembrane protein known as the canalicular multi-organic anion transporter, absent in patients with the Dubin-Johnson syndrome, an autosomal recessive liver disorder characterized by chronic conjugated hyperbilirubinemia. cMOAT/MRP2 is closely related to the multidrug resistance related protein MRP, and cMOAT/MRP2 overexpression has been observed in a subset of cisplatin resistant cell lines. M2I-4 was raised against a bacterial fusion protein of cMOAB/MRP2, containing amino acids 215-310 of the protein. M2I-4 did not cross react with the human MDR1, MRP1, MRP3 and MRP5 gene products.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
M2I-4
Concentration:
250 ug/ ml
Storage buffer:
Serum free tissue culture supernatant with 0.7% BSA and 0.1% sodium azide
The antibody was selected after immunization with a fusion protein consisting of Gluthathione S Transferase and a fragment of MDR3 P-gp comprising amino acid 629 - 692. P3II-26 reacts with an internal epitope of MDR3 P-gp. P3II-26 does not cross-react with the human MDR1 P-gp.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
P3II-26
Concentration:
100 ug/ ml
Format:
Protein G purified
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Scheffer G et al. Cancer Res 2000; 60: 5269-5277
References 2:
Hooiveld GJ et al. Gastroenterology 1999; 117; 678-687
The antibody reacts with a conserved cytoplasmic epitope of the plasma membrane-associated 170-180 kD glycoprotein, the expression of which is strongly correlated with the degree of multi-drug-resistance (MDR) derived MDR cell lines and human MDR cell lines, including cell lines derived from lung, ovaries and B cell lymphomas. Target species: Human, cross-reaction: Chinese hamster. No cross-reaction: mouse and rat.
The expression of MDM2 is itself, induced by p53 and may be a way for p53 to self-regulate its activity during the normal cell cycle. However, overexpression of MDM2 results in the loss of p53-regulated growth control and consequently, deregulated cell proliferation. MDM2 also binds to the Retinoblastoma tumor suppressor protein (Rb) and inhibits its growth regulatory function. MDM2 can directly augment proliferation by binding to two transcription factors E2F1 and DP1 and stimulating the activity of the S-phase inducing E2F1/DP1 heterodimer. MDM2 migrates at a reduced molecular weight of ~95 kDa. The SMP14 clone has been reported to recognize human, mouse and rat MDM2 while exhibiting a slight cross-reactivity with cytokeratins 6, 14 and 16 in some experimental systems. In the immunoprecipitation application, SMP14 has been reported to precipitate MDM2 and p53-MDM2 complexes. MCF7 human breast carcinoma cells (ATCC HTB-22) and NIH/3T3 mouse fibroblasts (ATCC CRL-1658) are suggested as western blot and immunoprecipitation positive controls. SMP14 has been reported to be useful for the immunohistochemical staining of acetone-fixed, frozen sections and of formalin-fixed, paraffin-embedded tissue sections. In addition to a nuclear staining of MDM2, cytoplasmic staining may also be observed which is likely to be attributable to the slight cross reactivity of the SMP14 clone with cytokeratins. Control tisse Breat carcinoma. Staining Nuclear
Monoclonal antibody MNA.1 (formerly known as 5D3-F7) recognizes human natural and recombinant monocyte chemotactic protein-1 (MCP-1). Monocyte chemotactic protein-1 (MCP-1) is a 11 kDa protein belonging to the CC subgroup of the chemokine superfamily, which stimulate the migration of monocytic cells. In contrast, the CXC chemokines predominantly activate polymorphonuclear leukocytes. The coordinated synthesis and release of MCP-1 plays a central role in both acute and chronic inflammatory processes by controlling the influx of phagocytic cells. Furthermore, their state of activation is in concert with primary inflammatory cytokines, such as IL-1, TNF-a, and IL-6. A selective accumulation of MCP-1 in the cerebrospinal fluid (CSF) of AIDS patients with cytomegalovirus encephalitis, but not with other opportunistic infections or primary lymphomas of the central nervous system , has been described. Furthermore, the chemotactic activity of MCP-1 on monocytic cells has been suggested to play a role in psoriasis, rheumatoid arthritis and atherosclerosis. No cross-reactivity of mAb MNA.1 with other cytokines has been detected.
Mouse anti Human Mcm5 antibody, clone CRCT5.1 recognizes human Mcm-5 (minichromosome maintenance protein 5), also known as DNA replication licensing factor MCM5 or P1-CDC46. Mcm5 is a nuclear protein of ~95kDa with an important role in the control of DNA replication (Snyder et al. 2005).Immunocytochemical assessment of Mcm5 expression may be of value in improving the accuracy of cervical smear testing for the detection of malignancy (Murphy et al. 2004).
Mouse anti Human MCM2 antibody, clone CRCT2.1 recognizes human DNA replication licensing factor MCM2, also known as Mcm2, Nuclear protein BM28 or mini chromosome maintenance protein-2. Mcm-2 is a 904 amino acid ~125kDa nuclear protein involved in the control of DNA replication.Mouse anti Human MCM2 antibody, clone CRCT2.1 has been used successfully for the detection of human MCM2 in ovarian adenocarcinoma by immunohistochemistry on formalin fixed, paraffin embedded tissues (Gakiopoulou et al. 2007).
Mannose Binding Lectin (MBL) also called mannose- or mannan-binding protein (MBP) is a member of the group of collectins. MBL is an oligomeric lectin that recognizes carbohydrates as mannose and N-acetylglucosamine on pathogens. MBL contains a cysteine rich, a collagen like and a carbohydrate recognition domain. It forms a complex with C1r/C1s like serine proteases designated MASPs that proteolytically cleave C4, C2 and C3. MBL is able to activate the complement pathway independent of the classical and alternative complement activation pathways. The MBL-MASP pathway (better known as the lectin pathway) is antibody and C1q-independent. MBL exhibits complement-dependent antibacterial activity and acts directly as an opsonic and therefore plays an important role in innate immunity. MBL is synthesized by hepatocytes and has been isolated from the liver or serum of various vertebrate species.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
3,00E+07
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Matsushita; M et al. Biochem Biophys Res Commun 1992; 183: 645
References 2:
Hisano, S et al Am J Kidney Dis 2001, 38: 1082
References 3:
Vries de; B et al. Am J Pathol 2004; 165: 1677
References 4:
Nauta A et al. Eur J Immunol 2003; 33 : 2853
References 5:
Nauta A et al. J Immunol 2004; 173: 3044
Cookies:
X
We use cookies to help personalise and improve your web experience.
By using our website you consent to our use of cookies, some of which may have already been set on your device.
View our Cookie Policy to learn more.
