AKT1 is a highly selective inward-rectifying potassium channel located in cell membrane, that mediates potassium uptake by plant roots in response to low potassium conditions.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide derived from Arabidopsis thaliana AKT1 Q38998 , At2g26650
In the work of Honsbein et al, 125I has been used for detection of KC1 since this was the only way to get enough signal after 2-phase partitioning, ECL+ has been used with the protein after expression in Sf9 insect cells (1: 1000 primary antibody dilution) and in yeast with no problem (single band detected), but these are relatively high expression systems, In native plant material ion channels are expressed in ridiculously small quantities (a few hundred proteins per cell)
Application Details:
1 : 50 with 125I (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 100 l of sterile water
Molecular Weight:
96,9 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Safiarian et al. (2015). Lost in traffic? The K+ channel of lily pollen, LilKT1, is detected at the endomembranes inside yeast cells, tobacco leaves and lily pollen. Front. Plant Sci. | doi: 10.3389/fpls.2015.00047.Honsbein et al. (2009). A tripartite SNARE-K+ channel complex mediates in channel-dependent K+ nutrition in Arabidopsis. The Plant Cell 21:2859-2877.
Special application note:
For detection images please, refer to the publication belowAntibody detects native and recombinant AKT1
Alb3.2 (Inner membrane ALBINO3-like protein 2, chloroplastic) is involved in the assembly of the light-harvesting complex and interacts with reaction center polypeptides of PSI and PSII.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Expected Species:
Scenedesmus sp. PABB004
Immunogen:
6xHis tagged, recombinant C-terminal part of Chlamydomonas reinhardtii Alb3.2, UniProt Q8LKI3. Chosen sequence is not conserved in Alb3.1.
Can be provided with ProClin, if requested. For Western blot image, check the original publication G hre et al. 2006.
Application Details:
1: 5000 - 1 : 10 000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water.
Molecular Weight:
44.7 kDa
Not reactive in:
Arabidopsis thaliana, Zea mays
Selected references:
Gohre et al (2006). One of two alb3 proteins is essential for the assembly of the photosystems and for cell survival in Chlamydomonas. Plant Cell. 2006 Jun;18(6):1454-66. doi: 10.1105/tpc.105.038695. Epub 2006 May 5. PMID: 16679460; PMCID: PMC1475496.
Alcohol dehydrogenase is an isozyme which preferentially catalyzes the conversion of primary unbranched alcohols to a corresponding aldehydes. Alternative names: Alcohol dehydrogenase I, YADH-1
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Saccharomyces cerevisiae
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
Alcohol dehydrogenase isolated and purified from UniProt: P00330
Applications:
ELISA (ELISA), Dot blot (Dot), Indirect immunofluorescence (indirect IF), Western blot (WB)
Aldehyde dehydrogenase in an enzyme involved in synthesis of acetate from ethanol. Alternative name: Aldehyde dehydrogenase 1, mitochondrial
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Saccharomyces cerevisiae
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
Aldehyde dehydrogenase isolated and purified from Saccharomyces cerevisiae, UniProt: P22281
Applications:
ELISA (ELISA), Dot blot (Dot), Immunocytochemistry (IHC), Western blot (WB)
Aldehyde dehydrogenase in an enzyme involved in synthesis of acetate from ethanol. Alternative name: Aldehyde dehydrogenase 1, mitochondrial
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Saccharomyces cerevisiae
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
Aldehyde dehydrogenase isolated and purified from Saccharomyces cerevisiae, UniProt: P22281
Applications:
ELISA (ELISA), Dot blot (Dot), Indirect immunofluorescence (indirect IF), Western blot (WB)
Fructose-1,6 bisphosphate aldolase (ALD) is an enzyme catalazying a key reaction of glycolysis and energy production, converting D-fructose- 1,6-bisphospate into dihydroxyacetone phosphate and D-glyceraldehyde-3-phosphate. This enzyme is present in plant and animal tissues. Plant enzyme is a class I aldolase which does not require a bivalent metal cofactor. It is located to outer mitochondrial membrane.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
This product can be sold containing ProClin if requested
Application Details:
1 : 5000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water
Molecular Weight:
38 | 38 kDa
Not reactive in:
Synechocystis sp.
Selected references:
Wang et al. (2018). iTRAQ-based quantitative proteomics analysis of an immature high-oleic acid near-isogenic line of rapeseed. Molecular Breeding January 2018, 38:2.Kamies et al. (2017). A Proteomic Approach to Investigate the Drought Response in the Orphan Crop Eragrostis tef. Proteomes. 2017 Nov 15;5(4). pii: E32. doi: 10.3390/proteomes5040032.Foley et al. (2017). A Global View of RNA-Protein Interactions Identifies Post-transcriptional Regulators of Root Hair Cell Fate.Dev Cell. 2017 Apr 24;41(2):204-220.e5. doi: 10.1016/j.devcel.2017.03.018.Parveen et al. (2016). Chickpea Ferritin CaFer1 Participates in Oxidative Stress Response, and Promotes Growth and Development. Sci Rep. 2016 Aug 9;6:31218. doi: 10.1038/srep31218.Yam et al. (2016). Characterization of the Plasmodium Interspersed Repeats (PIR) proteins of Plasmodium chabaudi indicates functional diversity. Sci Rep. 2016 Mar 21;6:23449. doi: 10.1038/srep23449.Dixit (2015). Sulfur alleviates arsenic toxicity by reducing its accumulation and modulating proteome, amino acids and thiol metabolism in rice leaves. Sci Rep. 2015 Nov 10;5:16205. doi: 10.1038/srep16205.Vera-Estrella et al. (2014). Comparative 2D-DIGE analysis of salinity responsive microsomal proteins from leaves of salt-sensitive Arabidopsis thaliana and salt-tolerant Thellungiella salsuginea. J Proteomics. 2014 Jun 2. pii: S1874-3919(14)00288-7. doi: 10.1016/j.jprot.2014.05.018.
Mouse anti-Aldolase C Monoclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Aldolases are glycolytic enzyme that catalyzes the reversible aldol cleavage of fructose 1,6-bisphosphate and fructose-1-phosphate to dihydroxyacetone phosphate and either glyceraldehyde 3-phosphate or glyceraldehyde, respectively. Three aldolase isozymes are found in mammals specifically aldolases A, B, and C, each of which is encoded by a separate gene. Aldolase A is generally considered to be a muscle enzyme. Northern analysis of cultured cells suggests that it is present in both neurons and glia (1). Aldolase B is considered to be a liver-specific enzyme and it is transcriptionally activated by signals from hormones and dietary factors (2). In the adult, aldolase C is the brain-specific isozyme, with low but detectable activity in fetal tissues (1, 3-6). Aldolase C shares 81% amino acid identity with aldolase A and 70% identity with aldolase B. Earlier studies using isozyme-specific antibodies report its location in gray matter astrocytes and cells of the pia mater (5, 8). In situ hybridization of mouse central nervous system using isozyme-specific probes revealed that aldolase A and C are expressed in complementary cell types: aldolase A mRNA is found in neurons; aldolase C message is detected in astrocytes, some cells of the pia mater, and Purkinje cells (9). Aldolase C can in some situations be used as an astrocyte marker. However Purkinje cells of the cerebellum contain high levels of the enzyme, so the enzyme is not totally astrocyte specific.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Bovine,Human,Mouse,Pig,Rat
Immunogen:
N-terminal 20 amino acids of aldolase C protein, MPHSYPALSAEQKKELSDIA
Applications:
ICC,WB
Clone number:
4A9
Antibody Isotype:
IgG1
Application Details:
Western Blotting (WB) and Immunocytochemistry (ICC). A dilution of 1:1,000 - 1:2,000 is recommended for WB. A dilution of 1:500 - 1:1,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Brain-type aldolase, Fructose-bisphosphate aldolase C
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The antibody reacts with a 40 kDa band by Western blot on a crude bovine cerebellum homogenate. It has also been used successfully for immunocytochemistry.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Aleu protein may play a role in proteolysis leading to mobilization of nitrogen during senescence and starvation. Catalytic activity : Hydrolysis of proteins, acting as an aminopeptidase (notably, cleaving Arg-|-Xaa bonds) as well as an endopeptidase. Cellular localisation: vacuole. Alternative names: Senescence-associated gene product 2
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 2 mg/ml.
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica oleracea, Camelina sativa, Capsella rubella, Eutrema salsugineum, Raphanus sativus Species of your interest not listed? Contact us
Immunogen:
Recombinant, His6-tagged, ALEU protein from Arabidopsis thaliana, UniProt: Q8H166, TAIR: At5g60360
Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Molecular Weight:
38,9 | 24 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Takagi et al. et al. (2013). MAIGO5 functions in protein export from Golgi-associated endoplasmic reticulum exit sites in Arabidopsis. Plant Cell. 2013 Nov;25(11):4658-75. doi: 10.1105/tpc.113.118158. (Western blot, Arabidopsis thaliana)Ueda et al. (2006). AtVAM3 is required for normal specification of idioblasts, myrosin cells. Plant Cell Physiol. 2006 Jan;47(1):164-75. doi: 10.1093/pcp/pci232. (Western blot, Arabidopsis thaliana)
Alkaline phosphatase is an enzyme which is involved in dephosphorylation process. Found in periplasmic space in Escherichia coli. Alternative name: APase
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Escherichia coli
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
Alkaline phosphatase isolated and purified from Escherichia coli, UniProt: P00634
Applications:
Dot blot (Dot), ELISA (ELISA), Indirect immunofluorescence (indirect IF), Western blot (WB)
Alkaline phosphatase is an enzyme which is involved in dephosphorylation process. Found in periplasmic space in Escherichia coli. Alternative name: APase
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Escherichia coli
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
Alkaline phosphatase isolated and purified from Escherichia coli, UniProt: P00634
Applications:
Dot blot (Dot), ELISA (ELISA), Indirect immunofluorescence (indirect IF), Western blot (WB)
Anaplastic Lymphoma Kinase (ALK) is a receptor tyrosine kinase which plays a role in brain and nervous system development. ALK is typically expressed at low levels in regions of the developing central and peripheral nervous system, such as the neonatal brain and spinal cord. The most common genetic alterations of this gene are chromosomal translocations, which result in multiple ALK fusion proteins that are involved in tumourigenesis, as in the case of anaplastic large cell lymphoma (ALCL), lung adenocarcinoma, and inflammatory myofibroblastic tumours. Aberrant ALK expression is also found in other tumours such as familial neuroblastoma, non-small cell lung carcinoma (NSCLC), and brain cancers.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC509
Antibody Isotype:
IgG2b
GMDN Code:
56791
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Anaplastic Large Cell Lymphoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Anaplastic Lymphoma Kinase (ALK) is a receptor tyrosine kinase which plays a role in brain and nervous system development. ALK is typically expressed at low levels in regions of the developing central and peripheral nervous system, such as the neonatal brain and spinal cord. The most common genetic alterations of this gene are chromosomal translocations, which result in multiple ALK fusion proteins that are involved in tumourigenesis, as in the case of anaplastic large cell lymphoma (ALCL), lung adenocarcinoma, and inflammatory myofibroblastic tumours. Aberrant ALK expression is also found in other tumours such as familial neuroblastoma, non-small cell lung carcinoma (NSCLC), and brain cancers.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC509
Antibody Isotype:
IgG2b
GMDN Code:
56791
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Anaplastic Large Cell Lymphoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
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