Our Immunoplates are designed for the capture of exosomes from any biological sample. Our plates are a standard 96-well format allowing assays to be conducted as singles and/or multi-well. This enables easy optimisation of a wide range of sandwich ELISA assays or other downstream applications, such as RNA extraction and proteomic analyses. Transparent, white and black plates are available depending on the downstream detection approach (colorimetric, luminometric and fluorimetric). Uncoated and/or covalently-coated plates are also available if required.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Our Immunoplates are designed for the capture of exosomes from any biological sample. Our plates are a standard 96-well format allowing assays to be conducted as singles and/or multi-well. This enables easy optimisation of a wide range of sandwich ELISA assays or other downstream applications, such as RNA extraction and proteomic analyses. Transparent, white and black plates are available depending on the downstream detection approach (colorimetric, luminometric and fluorimetric). Uncoated and/or covalently-coated plates are also available if required.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Purified and lyophilized Microvesicles (MV) are obtained from cell conditioned media. Diff erent EVs are separated by size using tangential flow filtration (TFF) and subsequantly purified by size exclusion chromatography (SEC) and microfiltration. Isolated vesicles are quantifi ed and validated for overall protein content, size distribution and particle number by NTA (Nanoparticles Tracking Analysis) with Zetaview analyzer (Particle Metrix).
Product Type:
Lyophilized microvesicles
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted microvesicles: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Purified and lyophilized Microvesicles (MV) are obtained from cell conditioned media. Diff erent EVs are separated by size using tangential flow filtration (TFF) and subsequantly purified by size exclusion chromatography (SEC) and microfiltration. Isolated vesicles are quantifi ed and validated for overall protein content, size distribution and particle number by NTA (Nanoparticles Tracking Analysis) with Zetaview analyzer (Particle Metrix).
Product Type:
Lyophilized microvesicles
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted microvesicles: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Purified and lyophilized Microvesicles (MV) are obtained from cell conditioned media. Diff erent EVs are separated by size using tangential flow filtration (TFF) and subsequantly purified by size exclusion chromatography (SEC) and microfiltration. Isolated vesicles are quantifi ed and validated for overall protein content, size distribution and particle number by NTA (Nanoparticles Tracking Analysis) with Zetaview analyzer (Particle Metrix).
Product Type:
Lyophilized microvesicles
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted microvesicles: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Purified and lyophilized Microvesicles (MV) are obtained from cell conditioned media. Diff erent EVs are separated by size using tangential flow filtration (TFF) and subsequantly purified by size exclusion chromatography (SEC) and microfiltration. Isolated vesicles are quantifi ed and validated for overall protein content, size distribution and particle number by NTA (Nanoparticles Tracking Analysis) with Zetaview analyzer (Particle Metrix).
Product Type:
Lyophilized microvesicles
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted microvesicles: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Purified and lyophilized Microvesicles (MV) are obtained from cell conditioned media. Diff erent EVs are separated by size using tangential flow filtration (TFF) and subsequantly purified by size exclusion chromatography (SEC) and microfiltration. Isolated vesicles are quantifi ed and validated for overall protein content, size distribution and particle number by NTA (Nanoparticles Tracking Analysis) with Zetaview analyzer (Particle Metrix).
Product Type:
Lyophilized microvesicles
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted microvesicles: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Purified and lyophilized Microvesicles (MV) are obtained from cell conditioned media. Diff erent EVs are separated by size using tangential flow filtration (TFF) and subsequantly purified by size exclusion chromatography (SEC) and microfiltration. Isolated vesicles are quantifi ed and validated for overall protein content, size distribution and particle number by NTA (Nanoparticles Tracking Analysis) with Zetaview analyzer (Particle Metrix).
Product Type:
Lyophilized microvesicles
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted microvesicles: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Purified and lyophilized Microvesicles (MV) are obtained from cell conditioned media. Diff erent EVs are separated by size using tangential flow filtration (TFF) and subsequantly purified by size exclusion chromatography (SEC) and microfiltration. Isolated vesicles are quantifi ed and validated for overall protein content, size distribution and particle number by NTA (Nanoparticles Tracking Analysis) with Zetaview analyzer (Particle Metrix).
Product Type:
Lyophilized microvesicles
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted microvesicles: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Purified and lyophilized Microvesicles (MV) are obtained from cell conditioned media. Diff erent EVs are separated by size using tangential flow filtration (TFF) and subsequantly purified by size exclusion chromatography (SEC) and microfiltration. Isolated vesicles are quantifi ed and validated for overall protein content, size distribution and particle number by NTA (Nanoparticles Tracking Analysis) with Zetaview analyzer (Particle Metrix).
Product Type:
Lyophilized microvesicles
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted microvesicles: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Purified and lyophilized Microvesicles (MV) are obtained from cell conditioned media. Diff erent EVs are separated by size using tangential flow filtration (TFF) and subsequantly purified by size exclusion chromatography (SEC) and microfiltration. Isolated vesicles are quantifi ed and validated for overall protein content, size distribution and particle number by NTA (Nanoparticles Tracking Analysis) with Zetaview analyzer (Particle Metrix).
Product Type:
Lyophilized microvesicles
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted microvesicles: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Purified and lyophilized Microvesicles (MV) are obtained from cell conditioned media. Diff erent EVs are separated by size using tangential flow filtration (TFF) and subsequantly purified by size exclusion chromatography (SEC) and microfiltration. Isolated vesicles are quantifi ed and validated for overall protein content, size distribution and particle number by NTA (Nanoparticles Tracking Analysis) with Zetaview analyzer (Particle Metrix).
Product Type:
Lyophilized microvesicles
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted microvesicles: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Purified and lyophilized Microvesicles (MV) are obtained from cell conditioned media. Diff erent EVs are separated by size using tangential flow filtration (TFF) and subsequantly purified by size exclusion chromatography (SEC) and microfiltration. Isolated vesicles are quantifi ed and validated for overall protein content, size distribution and particle number by NTA (Nanoparticles Tracking Analysis) with Zetaview analyzer (Particle Metrix).
Product Type:
Lyophilized microvesicles
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted microvesicles: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Immunobeads are coupled with antibodies that allows for exosome isolation from cell supernatants of human cell lines. Latex immunobeads can be easily recovered after incubation using a centrifuge. Exosomes captured on immunobeads can be used directly protein and mRNA/miRNA profiling.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Immunobeads are coupled with antibodies that allows for exosome isolation from cell supernatants of human cell lines. Latex immunobeads can be easily recovered after incubation using a centrifuge. Exosomes captured on immunobeads can be used directly protein and mRNA/miRNA profiling.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Immunobeads are coupled with antibodies that allows for exosome isolation from human biofluids (tested for plasma, serum, urine). Latex immunobeads can be easily recovered after incubation using a centrifuge. Exosomes captured on immunobeads can be used directly protein and mRNA/miRNA profiling.
Starting material: Recommended starting sample volume from 0.5ml of sample up to 1ml. Unfractionated plasma sample can be directly used for capture. Concentrated urine samples are recommended prior capture according to our suggested protocol.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Immunobeads are coupled with antibodies that allows for exosome isolation from human biofluids (tested for plasma, serum, urine). Latex immunobeads can be easily recovered after incubation using a centrifuge. Exosomes captured on immunobeads can be used directly protein and mRNA/miRNA profiling.
Starting material: Recommended starting sample volume from 0.5ml of sample up to 1ml. Unfractionated plasma sample can be directly used for capture. Concentrated urine samples are recommended prior capture according to our suggested protocol.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Immunobeads are coupled with antibodies that allows for exosome isolation from cell supernatants of human cell lines. Latex immunobeads can be easily recovered after incubation using a centrifuge. Exosomes captured on immunobeads can be used directly protein and mRNA/miRNA profiling.
Starting material: Concentrated cell culture supernatant samples (10X in spin concentrator) are recommended prior capture according to our suggested protocol.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Immunobeads are coupled with antibodies that allows for exosome isolation from cell supernatants of human cell lines. Latex immunobeads can be easily recovered after incubation using a centrifuge. Exosomes captured on immunobeads can be used directly protein and mRNA/miRNA profiling.
Starting material: Concentrated cell culture supernatant samples (10X in spin concentrator) are recommended prior capture according to our suggested protocol.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Our Immunoplates are designed for the capture of exosomes from any biological sample. Our plates are a standard 96-well format allowing assays to be conducted as singles and/or multi-well. This enables easy optimisation of a wide range of sandwich ELISA assays or other downstream applications, such as RNA extraction and proteomic analyses. Transparent, white and black plates are available depending on the downstream detection approach (colorimetric, luminometric and fluorimetric). Uncoated and/or covalently-coated plates are also available if required.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Our Immunoplates are designed for the capture of exosomes from any biological sample. Our plates are a standard 96-well format allowing assays to be conducted as singles and/or multi-well. This enables easy optimisation of a wide range of sandwich ELISA assays or other downstream applications, such as RNA extraction and proteomic analyses. Transparent, white and black plates are available depending on the downstream detection approach (colorimetric, luminometric and fluorimetric). Uncoated and/or covalently-coated plates are also available if required.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Our Immunoplates are designed for the capture of exosomes from any biological sample. Our plates are a standard 96-well format allowing assays to be conducted as singles and/or multi-well. This enables easy optimisation of a wide range of sandwich ELISA assays or other downstream applications, such as RNA extraction and proteomic analyses. Transparent, white and black plates are available depending on the downstream detection approach (colorimetric, luminometric and fluorimetric). Uncoated and/or covalently-coated plates are also available if required.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
EXO-Prep is a new and efficient method for exosome isolation from biofluids (i.e plasma serum and urine) and from cell culture supernatants. Exosomes are quicky isolated from your sample by an incubation step with EXO-Prep precipitation reagent. Exosome pellet is subsequently collected by one-step centrifugation and can be easily resuspended in PBS 1X. Solubilized exosomes are suitable for different downstream analyses such as proteomic or nucleic acids marker profiling, NTA, electron microscopy etc.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
EXO-Prep is a new and efficient method for exosome isolation from biofluids (i.e plasma serum and urine) and from cell culture supernatants. Exosomes are quicky isolated from your sample by an incubation step with EXO-Prep precipitation reagent. Exosome pellet is subsequently collected by one-step centrifugation and can be easily resuspended in PBS 1X. Solubilized exosomes are suitable for different downstream analyses such as proteomic or nucleic acids marker profiling, NTA, electron microscopy etc.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilized Exosome Standards from Human Biofluids
Additional Info:
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
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