Glyceraldehyde-3-phosphate dehydrogenase is an enzyme that catalyzes the first step in glycolysis by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. This enzyme is essential for the carbohydrate metabolism and the maintenance of cellular ATP levels. Synonyme gene names: GAPC, GAPDH.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store at 4 C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Use of this antibody as a loading control should be supported with specific experimental data
Application Details:
1 : 1000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
37 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Zhu et al. (2020). The RALF1-FERONIA Complex Phosphorylates eIF4E1 to Promote Protein Synthesis and Polar Root Hair Growth. Mol Plant. 2020 May 4;13(5):698-716. doi: 10.1016/j.molp.2019.12.014..
Glutathione-S-transferase (GST1) is an enzyme which belongs to GST superfamily and has a transferase activity. It is coded by a gene GSTS1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Expected Species:
Volvox carteri Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from Glutathione-S-transferase protein sequence from Chlamydomonas reinhardtii, UniProt: A8JBA7
Roach et al. (2018). Distress and eustress of reactive electrophiles and relevance to light stress acclimation via stimulation of thiol/disulphide-based redox defences. Free Radic Biol Med. 2018 Mar 18. pii: S0891-5849(18)30134-5. doi: 10.1016/j.freeradbiomed.2018.03.030.Kumar and Chattopadhyay (2018). Glutathione modulates the expression of heat shock proteins via the transcription factors BZIP10 and MYB21 in Arabidopsis. J Exp Bot. 2018 Jun 27;69(15):3729-3743. doi: 10.1093/jxb/ery166.
SBPase (Sedoheptulose-1,7-bis phosphatase) is a chloroplast enzyme involved in the carbon reduction of the Calvin cycle, part of carbohydrate metabolism.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Li et al. (2021) Did breeding alter the light environment, photosynthetic apparatus and photosynthetic capacity of wheat leaves? J Exp Bot. 2021 Nov 10:erab495. doi: 10.1093/jxb/erab495. Epub ahead of print. PMID: 34758079.Hammel et al. (2020) Photosynthesis in Chlamydomonas reinhardtii and Has No Effect on the Abundance of Other Calvin-Benson Cycle Enzymes. Front Plant Sci. 2020 Jun 23;11:868. doi: 10.3389/fpls.2020.00868. PMID: 32655601; PMCID: PMC7324757.Fukayama et al. (2018). Expression level of Rubisco activase negatively correlates with Rubisco content in transgenic rice. Photosynth Res. 2018 May 30. doi: 10.1007/s11120-018-0525-9.Li et al. (2018). Comparative proteomic analysis of key proteins during abscisic acid-hydrogen peroxide-induced adventitious rooting in cucumber (Cucumis sativus L.) under drought stress. Journal of Plant Physiology Volume 229, October 2018, Pages 185-194.
RpoC1 (RNA polymerase beta' subunit (chloroplast)) is a DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
RpoB (RNA polymerase beta subunit (chloroplast)) is a DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonuceloside triphosphates as substrates.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Alloteropsis semialata, Cannabis sativa, Coleataenia prionitis, Digitaria exilis, Echinochloa crus-galli var. crus-galli , Eragrostis tef, Microlaena stipoides, Miscanthus sacchariflorus, Oryza sativa, Phragmites australis, Potamophila parviflora, Rhynchoryza subulata, Saccharum officinarum, Setaria italica, Sorghum bicolor, Stipa lipskyi, Sporobolus michauxianus, Triticum aestivum Species of your interest not listed? Contact us
Immunogen:
His-tagged, highly conserved fragment of Zea mays RpoB gi|540067377|gb|AGV02730.1| RNA polymerase beta subunit (chloroplast) [Zea mays subsp. mays], UniProt: A0A059Q6W3
RpoA | RNA polymerase alpha subunit (chloroplast) is a DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Zea maysSpecies of your interest not listed? Contact us
Immunogen:
His-tagged, highly conserved fragment of Zea mays RpoA UniProt: P09562
Ji et al. (2020). A fully assembled PEP complex detected in etioplasts and proplastids in Arabidopsis. Physiol Plant. 2020 Nov 5. doi: 10.1111/ppl.13256.
Protein belongs to DNA photolyases and functions in DNA repair.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Lyophilized antibody can be stored at -20 °C for up to 3 years. Re-constituted antibody can be stored at 4°Cfor several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Mucuna pruriens (A0A371HBY0), Noccaea caerulescens (A0A1J3JTQ8) Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from Arabidopsis thaliana DNA photolyase, UniProt: F4JSJ6, TAIR: AT4G25290, located towards C-terminal part of this protein
Protein belongs to DNA photolyases and functions in DNA repair.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide derived from Arabidopsis thaliana DNA photolyase, UniProt: F4JSJ6, TAIR: AT4G25290, loacted in the N-terminal part of the protein
SUN1,2 (nuclear envelope protein) is a member of the Sad1/UNC-84 (SUN)-domain proteins.SUN domain proteins are part of the cytoskeletal-nucleoskeletal bridging complexes. These proteins are localized to the nuclear envelope and are present as homomers and heteromers in vivo. Involved in maintaining the elongated nuclear shape of epidermal cells.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated synthetic peptide derived from SUN1,2 sequences of Arabidopsis thaliana SUN1 UniProt: Q9FF75, TAIR: AT5G04990, SUN2, UniProt: Q9SG79, TAIR: AT3G10730
For other species use product AS15 2857This antibody is not suitable for immunolocalization.
Application Details:
1 : 1000 (WB), 1 : 200 (ICC)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
51,5 kDa (SUN1); 49,9 kDa (SUN2)
Selected references:
Wang et al. (2019) OPENER Is a Nuclear Envelope and Mitochondria Localized Protein Required for Cell Cycle Progression in Arabidopsis. Plant Cell. 2019 Jul;31(7):1446-1465. doi: 10.1105/tpc.19.00033
Histone 3 (H3) located in nuclei, incorporated into chromatin. Present in nucleosome together with H2A, H2B and H4.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°C. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Proteasome-dependent degradation serves an essential role in the removal of a wide variety of key nuclear and cytosolic proteins. Substrates are targeted for proteolysis by the ubiquitin pathway before being degraded by the 26 S proteasome. RPN6 associates with an ATPase subunit of the 19S proteasome regulatory complex, AtS6A. Alternative names: 19S proteosome subunit 9, 26S proteasome non-ATPase regulatory subunit 11 homolog, AtRPN6.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Serrate RNA effector molecule is required for proper processing of primary miRNAs to miRNA. Also critical for the accumulation of the trans-acting small interfering RNA (ta-siRNA).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Chicken
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Malus domestica, Nicotiana benthamina, Nicotiana tabacum, Saccharum hybrid cultivar NCo 376, Zea mays Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide chosen from Arabidopsis thaliana serrate protein sequence UniProt: Q9ZVD0,TAIR: At2g27100
RIQ1 is involved in regulation of thylakoid structure. This protein has a ubiquinone oxidoreductase domain and have an electron transfer motif.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Higher plants Species of your interest not listed? Contact us
At2g21960 is an integral component of thylakoid membrane.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Higher plants Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide, derived from Arabidopsis thaliana, UniProt: Q9SJ03, TAIR: AT2G21960
Proteasome-dependent degradation serves an essential role in the removal of a wide variety of key nuclear and cytosolic proteins. Substrates are targeted for proteolysis by the ubiquitin pathway before being degraded by the 26 S proteasome. RPN6 associates with an ATPase subunit of the 19S proteasome regulatory complex, AtS6A. Alternative names: 26S proteasome non-ATPase regulatory subunit 11 homolog, 19S proteosome subunit 9, AtS9 26S proteasome regulatory subunit RPN6, AtRPN6, 26S proteasome regulatory subunit S9 homolog.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Glycne soja , Gossypium arboreum, Medicago truncatula, Ornithogalum saundersiae, Oryza sativa, Populus trichocarpa, Ricinus communis, Solanum chacoense, Theobroma caco, Triticum urartu, Zea mays, Zostera marina Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from known sequences of RPN6 including Arabidopsis thaliana RPN6 sequence, UniProt: Q9LP45, TAIR: AT1G29150
ATG4 (Autophagy protein 4) is a protein with cyteine-type endopeptidase activity involved in autophagy process.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Technical details how to work with this antibody are provided here: P rez-P rez et al. (2016). Control of Autophagy in Chlamydomonas Is Mediated through Redox-Dependent Inactivation of the ATG4 Protease. Plant Physiol. 2016 Dec;172(4):2219-2234.
Chen et al. (2016). The role of nitric oxide signalling in response to salt stress in Chlamydomonas reinhardtii. Planta. 2016 Sep;244(3):651-69. doi: 10.1007/s00425-016-2528-0. Epub 2016 Apr 26.P rez-P rez et al. (2016). Control of Autophagy in Chlamydomonas Is Mediated through Redox-Dependent Inactivation of the ATG4 Protease. Plant Physiol. 2016 Dec;172(4):2219-2234.
Special application note:
This antibody is recognizing 25 ng of recombinant CrATG4This product can be sold containing ProClin if requested.
SUS1 (Sucrose synthase 1) is a sucrose-cleaving enzyme that provides UDP-glucose and fructose for various metabolic pathways. Alternative names: ASUS1, ATSUS1. SUS1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Bilska-Kos et al. (2020). Sucrose phosphate synthase (SPS), sucrose synthase (SUS) and their products in the leaves of Miscanthus giganteus and Zea mays at low temperature. Planta . 2020 Jul 16;252(2):23. doi: 10.1007/s00425-020-03421-2. Kleczkowski LA & Decker DD (2015) Sugar activation for production of nucleotide sugars as substrates for glycosyltransferases in plants. J. Appl. Glycosci. (in press).
UAGPase (UDP-GlcNAc pyrophosphorylase) is involved in the biosynthesis of UDP-glucosamine, an essential precursor for glycoprotein and glycolipid synthesis and is also used for regulatory protein modification in signaling pathways. The enzyme is localized in a cytoplasm. Alternative names: N-acetylglucosamine-1-phosphate uridylyltransferase 2, UDP-N-acetylgalactosamine diphosphorylase 2, UTP--glucose-1-phosphate uridylyltransferase 2, AGX.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Glycne soja, Medicago truncatula, Morus notabilis, Populus trichocarpa, Theobroma cacao, Zea mays, for more species, please inquire Species of your interest not listed? Contact us
Immunogen:
His-tagged full length recombinant UAGPase of Arabidopsis thaliana, overexpressed and purified from E.coli, UniProt:O64765,, TAIR:AT2G35020. Sequence used for immunization is conserved in both isoforms: UDP-N-acetylglucosamine diphosphorylase 1 (Q940S3) and (O64765)
This antibody is recognizing recombinant UAGPase at 0,25 pmol
Application Details:
1 : 10 000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
55,76 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Fern ndez-San Mill n et al. (2018). Physiological Performance of Transplastomic Tobacco Plants Overexpressing Aquaporin AQP1 into Chloroplast Membranes. J Exp. Bot. ery148, https://doi.org/10.1093/jxb/ery148.Kleczkowski LA & Decker DD (2015) Sugar activation for production of nucleotide sugars as substrates for glycosyltransferases in plants. J. Appl. Glycosci. 2015 Volume 62 Issue 2 Pages 25-36.
The PsbO protein is an extrinisic subunit of the water splitting photosystem II (PSII) complex. The protein is exposed on the luminal side of the thylakoid membrane, and is hihgly conserved in all known oxygenic photosynthetic organisms. Alternative names of PsbO1 include 33 kDa subunit of oxygen evolving system of photosystem II, OEC 33 kDa subunit, 33 kDa thylakoid membrane protein, manganese-stabilizing protein 1 and for PsbO2 33 kDa subunit of oxygen evolving system of photosystem II, OEC 33 kDa subunit, 33 kDa thylakoid membrane protein, manganese-stabilizing protein 2.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica napus, Capsella rubella, dinoflagellate, Triticum aestivum Species of your interest not listed? Contact us
This antibody is specific to PsbO2 and does not react with PsbO1 as confirmed on deletion mutant
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
33 | 30 kDa
Selected references:
Pipitone et al. (2021). A multifaceted analysis reveals two distinct phases of chloroplast biogenesis during de-etiolation in Arabidopsis. Elife. 2021 Feb 25;10:e62709. doi: 10.7554/eLife.62709. PMID: 33629953; PMCID: PMC7906606.Pralon et al. (2019). Plastoquinone homoeostasis by Arabidopsis proton gradient regulation 6 is essential for photosynthetic efficiency. Commun Biol. 2019 Jun 20;2:220. doi: 10.1038/s42003-019-0477-4.
The PsbO protein is an extrinisic subunit of the water splitting photosystem II (PSII) complex. The protein is exposed on the luminal side of the thylakoid membrane, and is hihgly conserved in all known oxygenic photosynthetic organisms. Alternative names of PsbO1 include 33 kDa subunit of oxygen evolving system of photosystem II, OEC 33 kDa subunit, 33 kDa thylakoid membrane protein, manganese-stabilizing protein 1 and for PsbO2 33 kDa subunit of oxygen evolving system of photosystem II, OEC 33 kDa subunit, 33 kDa thylakoid membrane protein, manganese-stabilizing protein 2.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica napus, Capsella rubella, Triticum aestivum Species of your interest not listed? Contact us
This antibody is specific to PsbO1 and does not react with PsbO2 as confirmed on a deletion mutant
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
33 | 30 kDa
Not reactive in:
Oryza sativa
Selected references:
Shanmugabalaji et al. (2018). Chloroplast Biogenesis Controlled by DELLA-TOC159 Interaction in Early Plant Development. Curr Biol. 2018 Aug 20;28(16):2616-2623.e5. doi: 10.1016/j.cub.2018.06.006.
FtsZ1 (cell division protein FtsZ homolog 1) is required for plastid cell division. Localization: pollen grain and plastids of vegetative cells. Alternative names: Chloroplast FtsZ, Protein accumulation and replication of chloroplasts 10, Protein plastid movement impaired4.FtsZ2 (cell visision protein FtsZ homolog 2) is required for plastid cell division. Present in two isoforms: FtsZ2-1 and FtsZ2-2. Alternative names: Plastid division protein FTSZ2.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Hordeum vulgare
Expected Species:
Chlamydomons reinhardtii, Cucumis sativus, Gentiana lutea, Glycine max, Gossypium arobretum, Jatropha manihot, Lilium longiflorum, Lupinus angustifolius, Manihot esculenta, Marchantia aquatica, Medicago truncatula, Morus notabilis, Nannochloropsis gaditana, Nicotiana tabacum, Oryza sativa, Physcomitrium patens, populus trichocarpa, Ricinus communis, Solanum lycopersicum, Sorgum bicolor, Theobroma cacao, Triticum uRatum, Zea mays, Yellow gentian, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
Recombinant part of Arabidopsis thalina FtsZ conserved in FtsZ1 Q42545 At5g55280 and FtsZ2 including FtsZ2-1 O82533, At2g36250 and FtsZ2-2 Q9LXJ0, At3g52750 and in a wide range of FtsZ proteins from other plant species.
Pex14p has a protein transporter activity and is involved in protein targeting into peroxisome. Submitted protein name: Genomic DNA, chromosome 5, P1 clone:MQB2
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Cucumis melo, Magnaporthe oryzaeSpecies of your interest not listed? Contact us
Immunogen:
Recombinant, soluble N-terminal domain of Arabidopsis thaliana Pex14p UniProt:Q9FE40, TAIR: At5g62810) that mediates PEX5 and PEX19 binding. The transmembrane and coiled-coil domains of PEX14 were replaced with a dual StrepII-His6 tag.
Antibodies will detect Pex14 protein in both light- and dark-grown seedlings grown on petri dishes and in rosette leaves of adult plants grown in soil
Application Details:
1 : 10 000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 25 l of sterile water
Molecular Weight:
55,5 | 75-65 kDa (depending on the gel system)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Llinas et al. (2022) An Arabidopsis pre-RNA processing8a (prp8a) missense allele restores splicing of a subset of mis-spliced mRNAs. Plant Physiol. 2022 Aug 1;189(4):2175-2192. doi: 10.1093/plphys/kiac221. PMID: 35608297.Calero-Munoz et al. (2019). Cadmium induces reactive oxygen species-dependent pexophagy in Arabidopsis leaves. Plant Cell Environ. 2019 Sep;42(9):2696-2714. doi: 10.1111/pce.13597.McLoughlin et al. (2018). Maize multi-omics reveal roles for autophagic recycling in proteome remodelling and lipid turnover. Nat Plants. 2018 Dec;4(12):1056-1070. doi: 10.1038/s41477-018-0299-2.Gonzalez et al. (2018). A pex1 missense mutation improves peroxisome function in a subset of Arabidopsis pex6 mutants without restoring PEX5 recycling. Proc Natl Acad Sci U S A. 2018 Apr 3;115(14):E3163-E3172. doi: 10.1073/pnas.1721279115.Vincent et al. (2017). A genome-scale analysis of mRNAs targeting to plant mitochondria: upstream AUGs in 5' untranslated regions reduce mitochondrial association. Plant J. 2017 Dec;92(6):1132-1142. doi: 10.1111/tpj.13749.
Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and is highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery. Heat shock cognate protein 70 (HSC70), is a highly conserved protein and a member of the family of molecular chaperones.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
KLH-conjugated synthetic peptide conserved in known higher plant HSC70 proteins including three isoforms of Arabidopsis thaliana HSC70-1 UniProt: F4KCE5 , HSC70-2 UniProt: A0A178UTH3 and HSC70-3 Uniprot: O65719 as well as heat shock inducible Hsp70 of Arabidopsis thaliana TAIR: AT3g12580/T2E22_110 and At1g16030 and AT3g12580/T2E22_110
Can be sold containing 0.1% ProClin if requested This antibody can be used as a marker of cytoplasmic fraction in tomato (Anfoka et al. 2015).Applied primary antibody dilution in western blot depends upon sensitivity of detection reagents (pico or femtogram for chemiluminescent detection).Immunoprecipitation protocol using Agrisera anti-Hsp70 cytosolic antibodies, see tab: protocols.
Application Details:
1 : 3000 - 1: 10 000, 5 g protein/well (WB), 2-3 l/protein extract of concentration 3-5 mg/ml
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
70 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Chong et al. (2022) The tomato OST1-VOZ1 module regulates drought-mediated flowering. Plant Cell. 2022 Apr 26;34(5):2001-2018. doi: 10.1093/plcell/koac026. PMID: 35099557; PMCID: PMC9048945.Bychkov et al. (2022) The role of PAP4/FSD3 and PAP9/FSD2 in heat stress responses of chloroplast genes. Plant Sci. 2022 Sep;322:111359. doi: 10.1016/j.plantsci.2022.111359. Epub 2022 Jun 20. PMID: 35738478.Cvetkovska et al. (2022) A constitutive stress response is a result of low temperature growth in the Antarctic green alga Chlamydomonas sp. UWO241. Plant, Cell & Environment, 45, 156– 177. https://doi.org/10.1111/pce.14203Wang et al. (2022) 17-(Allylamino)-17-demethoxygeldanamycin treatment induces the accumulation of heat shock proteins and alleviates senescence in broccoli. Postharvest Biology and Technology,Volume 186, 2022, 111818, ISSN 0925-5214, https://doi.org/10.1016/j.postharvbio.2021.111818.Kumari et al. (2021) In-depth assembly of organ and development dissected Picrorhiza kurroa proteome map using mass spectrometry. BMC Plant Biol. 2021 Dec 22;21(1):604. doi: 10.1186/s12870-021-03394-8. PMID: 34937558; PMCID: PMC8693493.
Special application note:
This product can be sold containing ProClin if requested
UPF0603 protein At1g54780, chloroplastic is located in thylakoid lumen. The protein seem to be required for high light tolerance of PSII. Sirpi et al. (2007) TLP18.3, a novel thylakoid lumen protein regulating photosystem II repair cycle. Biochem J; 406(3): 415-425
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Oryza sativa, Ricinus communis, Zea mays Species of your interest not listed? Contact us
Immunogen:
synthetic peptides amino acids 227-241 and amino acids 242-256 from Arabidopsis thaliana Tlp18.3 protein sequence TAIR: At1g54780)
Application note: in a gel without urea Tlp18,3 will run at 18 kDa, while in a gel with urea it runs at 25 kDa
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 µl of sterile water
Molecular Weight:
22 | 18.5 kDa (A. thaliana)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Zienkiewicz et al. (2013).Light intensity and quality stimulated Deg1-dependent cleavage of PSII components in the chloroplasts of maize. Plan Physiol Biochem. March 16.
APX plays a key role in plant antioxidant system by reducing hydrogen peroxide to water. Cellular localization includes chloroplast (tAPX and sAPX), cytosol (cAPX) and peroxisome (pAPX).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Brassica rapa subsp. oleifera Stromal APX; Glycine max, Glycine soja L-ascorbate peroxidase T, chloroplastic; Medicago truncatula thylakoid-bound APX; Mesembryanthemum crystallinum, Pisum sativum Chloroplast stromal ascorbate peroxidase 12; Solanum lycopersicum thylakoid-bound APX; Spinacia oleracea stromal APX; Theobroma cacao L-APX T isoform 3; Vitis viniferaSpecies of your interest not listed? Contact us
Immunogen:
BSA-conjugated synthetic peptide derived from Arabidopsis thaliana tAPX (thylakoidal ascorbate peroxidase) UniProt: Q42593-1, TAIR:At1g77490 and sAPX (stromal/mitochondrial ascorbate peroxidase) UniProt: Q42592-1 TAIR: At4g08390 Five out of twelve amino acids are also identical with cAPX1 (At1g07890), cAPX2 (At3g09640) and pAPX (At4g35000)
This product can be sold containing proclin if requested
Application Details:
1 : 2000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
25-38 kDa for A. thaliana
Not reactive in:
Algae, Helianthus annus, Marchantia polymorpha
Selected references:
Wang et al. (2022) Reciprocity between a retrograde signal and a putative metalloprotease reconfigures plastidial metabolic and structural states. Sci Adv. 2022 Jun 3;8(22):eabo0724. doi: 10.1126/sciadv.abo0724. Epub 2022 Jun 3. PMID: 35658042; PMCID: PMC9166295.Kucko et al. (2022) The acceleration of yellow lupine flower abscission by jasmonates is accompanied by lipid-related events in abscission zone cells, Plant Science, Volume 316, 2022,111173, ISSN 0168-9452, https://doi.org/10.1016/j.plantsci.2021.111173. (https://www.sciencedirect.com/science/article/pii/S0168945221003691)Jedelska et al. (2021) Protein S-nitrosation differentially modulates tomato responses to infection by hemi-biotrophic oomycetes of Phytophthora spp. Hortic Res. 2021 Feb 1;8(1):34. doi: 10.1038/s41438-021-00469-3. PMID: 33518717; PMCID: PMC7848004.Tokarz et al. (2021). Stem Photosynthesis-A Key Element of Grass Pea (Lathyrus sativus L.) Acclimatisation to Salinity. Int J Mol Sci. 2021 Jan 12;22(2):685. doi: 10.3390/ijms22020685. PMID: 33445673; PMCID: PMC7828162.Tokarz et al. (2020). Can Ceylon Leadwort ( Plumbago zeylanica L.) Acclimate to Lead Toxicity?-Studies of Photosynthetic Apparatus Efficiency. Int J Mol Sci. 2020 Mar 9;21(5):1866.doi: 10.3390/ijms21051866.
UniProt number:
Q42593 , Q42592 , Q05431 , Q1PER6 , Q42564
TAIR number:
AT1G77490
Research area:
Arabidopsis thaliana antibodies
Code:
Stress20
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