COX11 (cytochrome c oxidase assembly protein) , involved in respiratory chain complex IV assembly.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardii
Expected Species:
Physcomitrium patensSpecies of your interest not listed? Contact us
Immunogen:
Recombinant fragment of Chlamydomonas reinhardtii COX11 UniProt: A8JCS0
CGL78 is a protein conserved in the green lineage and predicted to be located in the chloroplast. Member of the Ycf54 superfamily.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Please, omitt SDS from transfer buffer and reduce transfer time to 45 min, Nitrocellulose membrane is recommended and SDS is omitted to allow this LMW protein to bind tighter to the membrane
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 200 l of sterile water
Molecular Weight:
24 kDa
Not reactive in:
Hordeum vulgare
Selected references:
Hsieh et al. (2013). The Proteome of Copper, Iron, Zinc, and Manganese Micronutrient Deficiency in Chlamydomonas reinhardtii. Mol Cell Proteomics. 2013 Jan;12(1):65-86. doi: 10.1074/mcp.M112.021840. Epub 2012 Oct 13.
Special application note:
Currently this antibody has not been confirmed to detect CGL78 protein in Arabidopsis thaliana. If you are interested to use this antibody in Arabidopsis, please, contact us.
eEF1B-beta1 protein belongs to a family of elongation factors, proteins which are involved in translational elongation. Alternative names: Elongation factor 1-delta 1, EF-1-delta 1
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Oryza sativa, Ricinus communis, Solanum tuberosum, Zea mays, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
recombinant eEF1B-beta1 protein from Arabidopsis thaliana with no affinity tag, P48006, At1g30230
No confirmed exceptions from predicted reactivity are currently known
Selected references:
McLoughlin et al. (2019) HSP101 Interacts with the Proteasome and Promotes the Clearance of Ubiquitylated Protein Aggregates. Plant Physiol. 2019 Aug;180(4):1829-1847. doi: 10.1104/pp.19.00263McLoughlin et al. (2016) Class I and II Small Heat Shock Proteins Together with HSP101 Protect Protein Translation Factors during Heat Stress. Plant Physiol. 2016 Oct;172(2):1221-1236.
Special application note:
Antibody is recognizing both proteins: elonagtion factor beta 1 and beta 2
The PsbI protein, previously named the 4.8-kDa protein, is encoded by the plastome. PsbI is a universal component of PSII and is highly conserved (e.g. there is 71% amino acid identicality between the Arabidopsis and Synechocystis 6803 proteins). The protein contains 36 to 38 amino acids in most species, with molecular masses ranging between 4.1 and 4.5 kDa. Synonymes: PSII-I, PSII 4.8 kDa protein
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Hordeum vulgare
Expected Species:
Cannabis sativa, Glycne max, Phaseolus vulgaris, Populus trichocarpa, Spinacia oleracea, Triticum aestivum, Zea mays Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from PsbI protein of Arabidopsis thaliana P62100, AtCg00080
Hackett et al. (2017). An Organelle RNA Recognition Motif Protein Is Required for Photosystem II Subunit psbF Transcript Editing. Plant Physiol. 2017 Apr;173(4):2278-2293. doi: 10.1104/pp.16.01623.
Hcf101 is a novel protein required for photosystem I biogenesis. Hcf101-1 is a high-chlorophyll-fluorescence hcf) Arabidopsis thaliana mutant that lacks photosystem I. In green tissue the level of this protein is stimulated by light and the protein has not been detected in roots. It has been localized in chloroplast stroma.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Immunolocalization has been done on isolated plastids, followed by separation into stroma and thylakoids
Application Details:
1 : 3000 (IL), 1 : 3000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 200 l of sterile water
Molecular Weight:
57 | 50,5 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Hu et al. (2017). The SUFBC2 D Complex is Required for the Biogenesis of All Major Classes of Plastid Fe-S Proteins. Plant J. 2017 Jan 19. doi: 10.1111/tpj.13483.Bigeard et al. (2014). Proteomic and phosphoproteomic analyses of chromatin-associated proteins from Arabidopsis thaliana. Proteomics. 2014 May 31. doi: 10.1002/pmic.201400072.
GDP-L-Galactose Phosphorylase is a histidine triad (HIT) enzyme of the Smirnoff-Wheeler pathway of ascorbic acid synthesis in plants. Encoded by VTC2 gene. The enzyme catalyzes the conversion of GDP-L-galactose to L-galactose 1-phosphate in a reaction that consumes inorganic phosphate and produces GDP.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Brassica oleracea var. italica, Brassica rapa var. komatsuna, Citrus limon, Nicotiana tabacum, Spinacia oleracea, Zea mays, Chlamydomonas reinhardtii
Expected Species:
Manihot esculenta, Sorghum bicolor, Oryza sativa, Physcomitrium patensSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjuated synthetic peptide derived from known GDP-L-Galactose Phosphorylase sequences, including Arabidopsis thaliana Q8LKQ7 (At4g26850) and Chlamydomonas reinhardtii
eEF1B-gamma1 protein belongs to a family of elongation factors, proteins which are involved in translational elongation. Alternative names: EF-1-gamma 1, eEF-1B gamma 1
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Oryza sativa, Solanum tuberosum, Zea mays, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
Recombinant eEF1B-gamma1 protein from Arabidopsis thaliana with no affinity tag,UniProt: O04487, TAIR: At1g09640
No confirmed exceptions from predicted reactivity are currently known
Selected references:
McLoughlin et al. (2019) HSP101 Interacts with the Proteasome and Promotes the Clearance of Ubiquitylated Protein Aggregates. Plant Physiol. 2019 Aug;180(4):1829-1847. doi: 10.1104/pp.19.00263McLoughlin et al. (2016) Class I and II Small Heat Shock Proteins Together with HSP101 Protect Protein Translation Factors during Heat Stress. Plant Physiol. 2016 Oct;172(2):1221-1236.
Special application note:
Antibody is recognizing elongation factor 1-gamma 1 and gamma 2
PsbTn (Tn protein of PSII) nuclear encoded protein involved in photosynthesis. Alternative names: photosystem II 5 kDa protein, chloroplastic, PSII-T, Nuclear encoded psbT.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Cicer arietinum, Glycine soja, Helianthus annuus, Medicago truncatula, Nicotiana attenuata, Nicotiana tabacum, Nicotiana sylvestris, Noccaea caerulescens, Petunia hybrida, Populus trichocarpa, Solanum chacoense, Solanum tuberosum Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide, derived from Arabidopsis thaliana PsbTn protein sequence, UniProt:Q39195, TAIR: AT3G21055
LMW proteins can sometimes interfere with chlorophyll, but most chlorophyll can be removed by precipitating sample in acetone before loading on a gel.Protocol: Add acetone to final concentration of 80% ice-cold acetone. Leave 10 minutes. Spin. Rresuspend pellet in solubilisation buffer and load on a gel.
Application Details:
1: 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 200 l of sterile water
Molecular Weight:
11 | 5 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Chen et al. (2019). The Low Molecular Mass Photosystem II Protein PsbTn is Important for Light Acclimation. Plant Physiol. Apr;179(4):1739-1753. doi: 10.1104/pp.18.01251.
OEP 75 or Toc75; Chloroplast outer envelope membrane protein from Pisum sativum (pea), Predicted to contain 3 POTRA domains at N-terminus. Believed to be the protein conducting channel of the Toc translocon and assembles as an 18 stranded -barrel (EMBO J. (1995) 14:11, 2436-2446); In Arabidopsis there are five members of this Family Toc75 (I-V), atToc75III is most closely related to psToc75. Additionally, it is structurally related to members of the bacterial surface antigen super-family including: OMA87; Outer membrane protein/protective antigen, (COG4775, COG4775) [Cell envelope biogenesis, outer membrane; YaeT; outer membrane protein assembly complex, (TIGR03303); FhaC; Hemolysin activation/secretion protein (COG2831) [Intracellular trafficking and secretion]
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Pisum sativum, some cross-reactivity was observed for cyanobacteria including: Synechocystis, Synechococcus and Thermosynechococcus
Expected Species:
Oryza sativa, Ricinus communis, Populus trichocarpa, Vitis viniferaSpecies of your interest not listed? Contact us
Immunogen:
psTOC75; Predicted POTRA Domain #1; Amino acids, 158-241; Expressed and purified in E. coli using the Impact System from NEB. Peptide confirmed by MALDI. Q43715
Applications:
Flow cytometry (Flow cyt), Immunolocalization (IL),Western blot (WB)
Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery. A killifish is a large family of various oviparous (egg-laying) cyprinodontiform fish including 1270 different species.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Chandra et al. (2012). Sustained high temperature increases the vitellogenin response to 17 alpha-ethynylestradiol in mummichog (Fundulus heteroclitus). Aquatic toxicology.
Special application note:
Chosen peptide sequence is also conserved in several fish species including: DQ202278.1 HSC70 Fundulus, DQ202279.1 HSP70-1 Fundulus, DQ202280.1 HSP70-2 Fundulus, BT059361.1 HSC70 Salmo salar (atlantic salmon), AB092839.2 HSP70 Carassius auratus (goldfish), BC056709.1 HSP70 Danio rerio (zebrafish) and other animal HSP70 proteins
eEF1B-beta protein belongs to a family of elongation factors, proteins which are involved in translational elongation. eEF1B-beta protein is localized in plant cytosol. Alternative names: EF-1-delta 2, elongation factor 1B-beta 2
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Zea mays
Expected Species:
Brassica campestris, Hordeum vulgare, Nicotiana attenuata, Oryza sativa Pisum sativum, Ricinus communisSpecies of your interest not listed? Contact us
Immunogen:
Recombinant eEF1B-beta protein from Arabidopsis thaliana with no affinity tag, UniProt: Q9SI20,TAIR: At2g18110
No confirmed exceptions from predicted reactivity are currently known
Selected references:
L zaro-Mixteco et al. (2012). The Absence of Heat Shock Protein HSP101 Affects the Proteome of Mature and Germinating Maize Embryos. J. Proteome Res. May 11, ahead of print.
Hsp101/ClpB is a member of HSP100 protein family. These proteins help protein aggregates formed during heat stress to fall apart to allow them to be refolded by other chaperones. In spite of expression during heat stress members of HSP100 protein family are also expressed during seed development.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4 C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Chicken
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Glycne max, Nicotiana tabacum, Vitis vinifera Species of your interest not listed? Contact us
Hsp17.6 belongs to a family of class I of a small heat shock proteins. They are induced once a plant cells are stressed by an increased temperature. The way small hsp proteins are protecting a living cell are not fully understood. They seem to be involved in chaperone functions by protecting other proteins from irreversible denaturation. Small hsp function also in a late seed maturation process.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4 C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Chicken
Species Reactivity:
Arabidopsis thaliana
Immunogen:
Recombinant protein derived from a sequence Arabidopsis thaliana HSP17.6 Ci (class one) UniProt: P13853, TAIR: At1g53540
There are six total class I genes, Essentially this antibody might react to some extent with all of them, But does not react with class II, organelle, or any other shsp classes
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Mao and Sun (2015). Arabidopsis seed-specific vacuolar aquaporins are involved in maintaining seed longevity under the control of ABSCISIC ACID INSENSITIVE 3. J Exp Bot. 2015 May 26. pii: erv244.
Enolase (2-phospho-D-glycerate hydrolase or phosphopyruvate dehydratase) is an essential glycolytic metalloenzyme. It is catalyzing the interconversion of 2-phosphoglycerate and phosphoenolpyruvate. Alternative name: Bifunctional enolase 2/transcriptional activator
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Helianthus annuus
Expected Species:
Brassica sp., Chlamydomonas reinhardii, Lycopersicum esculentum, Gossypium mexicanum, Nannochloropsis gaditana, Nicotiana tabacum, Oryza sativa, Physcomitrium patens, Populus balsamifera, Ricinus communis, Zea mays Species of your interest not listed? Contact us
Antibody is specific for the ENO2 isoform (cytosolic and active isoform), see data below
Application Details:
1 : 2000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 200 l of sterile water
Molecular Weight:
47.7 kDa (Arabidopsis thaliana)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Zhang et al. (2020). A moonlighting role for enzymes of glycolysis in the co-localization of mitochondria and chloroplasts. Nat Commun. 2020 Sep 9;11(1):4509.doi: 10.1038/s41467-020-18234-w. Zhang et al. (2018). Nitric oxide induces monosaccharide accumulation through enzyme S-nitrosylation. Plant Cell Environ. 2017 Sep;40(9):1834-1848. doi: 10.1111/pce.12989.Chen et al.(2009) System analysis of an Arabidopsis mutant altered in de novo fatty acid synthesis reveals diverse changes in seed composition and metabolic regulation. Plant Physiol.
Plastoglobules are lipoprotein particles which can be found in chloroplasts. They are generally believed to have a function of lipid storage. Recent data suggest that plastoglobules can be also metabolically active, taking part in tocopherol synthesis and likely other pathways. Immunogen: Alternative name AtPap1, fibrillin-1, probable plastid-lipid-associated protein 1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
(2021). Autophagy is required for lipid homeostasis during dark-induced senescence.Plant Physiology, 2021;, kiaa120Luo et al. (2015). Distinct carotenoid and flavonoid accumulation in a spontaneous mutant of Ponkan (Citrus reticulata Blanco) results in yellowish fruit and enhanced postharvest resistance. J Agric Food Chem. 2015 Sep 2.G mez-Arjona et al. (2014). Starch synthase 4 is located in the thylakoid membrane and interacts with plastoglobule-associated proteins in Arabidopsis. Plant J. 2014 Oct;80(2):305-16. doi: 10.1111/tpj.12633.
Special application note:
Cellular [compartment marker] of chloroplast plastoglobules.For IC samples were embedded in Lowicryl HM20 and sectioned into 100-nm-thick sections and placed on Formvar-coated gold slot grids. The sections were blocked for 20 min with a 5% (w/v) solution of nonfat milk in TBS plus 0.1%Tween 20 (TBST). Anti-PGL antibodies were diluted 1:20 in a solution of 2.5% nonfat milk in TBST at room temperature for 1 h. The sections were rinsed in a stream of TBS plus 0.5% Tween 20 and then transferred to the secondary antibody (anti-rabbit IgG 1:20 in TBST) conjugated to 10-nm gold particles for 1 h. images of localization can be found in Austin et al. (2006).
PsbY (Small subunit Y of PSII) is a manganese-binding polypeptide with L-arginine metabolizing enzyme activity. It is a component of the core of photosystem II. Alternative names: psbY-A1, L-AME, L-arginine.metabolizing enzyme.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Glycne soja , Medicago truncatula, Morus notabilis, Oryza sativa, Populus trichocarpa, Ricinus communi, Solanum chacoense, Spinacia oleracea, Theobroma cacao , Zea mays, Zostera marina Species of your interest not listed? Contact us
Von Sydow et al. (2016). The PsbY protein of Arabidopsis Photosystem II is important for the redox control of cytochrome b559. Biochim Biophys Acta. 2016 May 21. pii: S0005-2728(16)30536-9. doi: 10.1016/j.bbabio.2016.05.004.
Copper is an essential element for function of chloroplasts. It is a co-factor for superoxide dismutase (SOD) and for plastocyanin. Copper is transported to chloroplasts with a help of Copper Chaperones, which carry out the delivery of copper from transporters to targets. In the last step chaperone binds to a copper dependent enzyme and inserts the copper ions into its active site. The Arabidopsis thaliana gene AtCCS encodes a copper chaperone for SOD. The AtCCS protein was localized to chloroplasts where it may supply copper to the stromal Cu/ZnSOD.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Glycne max, Hordeum vulgare, Solanum tuberosum Species of your interest not listed? Contact us
Immunogen:
The antibody was raised to the Arabidopsis thaliana full length CCs protein Q4ZJI5, fused to His-tag. Overexpressed using pET28a vector in Bl21 E. coli (Cd+) and purified using His-column. After purification, the His tag was cleaved using thrombin and then theprotein was purified using Resource-Q column connected to HPLC.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Speth et al. (2013). RACK1 scaffold proteins influence miRNA abundance in Arabidopsis. Plant J. Aug 13.Abdel-Ghany et al (2005). .AtCCS is a functional homolog of the yeast copper chaperone Ccs1/Lys7. FEBS Lett. 11:2307-12.
HemH is ptotoporphyrin ferrochelatase of heme biosynthesis.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Protein used to elicit this ab shares ca. 50 % homology to Arabidopsis thaliana ferrochelatase 1 and 2.
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 200 l of sterile water
Molecular Weight:
50 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Masoumi et al. (2008). Complex formation between protoporphyrinogen IX oxidase and ferrochelatase during haem biosynthesis in Thermosynechococcus elongatus. Microbiol. 154: 3707-3714.
Ferredoxin-NADP reductase, leaf isozyme 1 (L-FNR1) plays a key role in regulating the relative amounts of cyclic and non-cyclic electron flow to meet the demands of the plant for ATP and reducing power. Localised in the chloroplast.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 1 mg/ml.
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Zea mays
Expected Species:
Hordeum vulgare, Oryza brachyantha, Saccharum sp., Setaria italica, Sorghum bicolorSpecies of your interest not listed? Contact us
Immunogen:
Purified recombinant maize leaf FNR1 protein UniProt: Q9SLP6, sharing homology with maize FNR2, FNR3 and Arabidopsis thaliana FNR1 Q9FKW6
The Glycine decarboxylase complex (GDC) is abundant in mitochondria matrix of C3 leaves and functions in photorespiratory carbon recovery. GDC enzyme can account for up to 50% of matrix protein, and is responsible for the most prominent metabolic activity in the mitochondria of illuminated leaves, photorespiration. GDC is a multienzyme complex composed of four component enzymes, the P-, H-, T-, and L-proteins and is responsible for the conversion of glycine produced in the peroxisome to serine in the mitochondria during photorespiratory cycle. The H-protein plays a key role as a mobile substrate that commutes between the other subunits, allowing its lipoic acid “arm” to visit the active sites of the other three components.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
This antibody can be used on total cell extract of Arabidopsis thaliana.
Application Details:
1 : 5 000 (TP), (WB)
Purity:
Total IgG. Protein G purified in PBS pH 7.4.
Reconstitution:
For reconstitution add 200 l of sterile water
Molecular Weight:
16 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Sch fer et al. (2021) Assessment of Mitochondrial Protein Topology and Membrane Insertion. Methods Mol Biol. 2022;2363:165-181. doi: 10.1007/978-1-0716-1653-6_13. PMID: 34545493.Przybyla-Toscano et al. (2021) Protein lipoylation in mitochondria requires Fe-S cluster assembly factors NFU4 and NFU5. Plant Physiol. 2021 Oct 28:kiab501. doi: 10.1093/plphys/kiab501. Epub ahead of print. PMID: 34718778.Guralnick et al. (2020). The Development of Crassulacean Acid Metabolism (CAM) Photosynthesis in Cotyledons of the C4 Species, Portulaca grandiflora (Portulacaceae). Plants (Basel). 2020 Jan 2;9(1). pii: E55. doi: 10.3390/plants9010055. (tissue printing)R thor et al. (2019). Arabidopsis seedlings display a remarkable resilience under severe mineral starvation using their metabolic plasticity to remain self-sufficient for weeks. Plant J. 2019 Mar 22. doi: 10.1111/tpj.14325.Lynch et al. (2017). Multifaceted plant responses to circumvent Phe hyperaccumulation by downregulation of flux through the shikimate pathway and by vacuolar Phe sequestration. Plant J. 2017 Dec;92(5):939-950. doi: 10.1111/tpj.13730. (Petunia hybrida cv. Mitchell)
Special application note:
Cellular [compartment marker] of mitochondrial matrix
VSP (Vegetative storage protein 1) may function as somatic storage protein during early seedling development. Expression of VSP1 is enhanced by wounding and protein is localized to vacuole.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
Full length, purified recombinant His6-tagged VSP1 of Arabidopsis thaliana UniProt: O49195, TAIR: At5g24780
Reactivity of this antibody to VSP2 has not been determined, Sequence conservation of VSP1 and VSP2 is 86 % therefore, it is most likely that this antibody will also recognize VSP2,
Application Details:
1: 1000 - 1: 2000 (WB)
Purity:
Total IgG, purified on Protein A in PBS, 50 % glycerol, filter sterilized.
Molecular Weight:
28 | 27 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Matsushima et al. (2002). An endoplasmic reticulum-derived structure that is induced under stress conditions in Arabidopsis.Plant Physiol. 2002 Dec;130(4):1807-14. doi: 10.1104/pp.009464.
Sulfite reductase (SiR) is an essential protein with sulfite reductase activity required in assimilatory sulfate reduction pathway during both primary and secondary metabolism and thus involved in development and growth. It is known as a DNA-binding protein that binds to both double-stranded and single-stranded DNA without significant sequence specificity to reversibly repress the transcriptional activity of chloroplast nucleoids by promoting DNA compaction and possibly regulate DNA replication. The sequence identity between maize and Arabidopsis SiR is 77%.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 4 mg/ml.
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Pisum sativum, Zea mays
Expected Species:
Dichanthelium oligosanthes, Panicum hallii, Setaria viridis, Sorghum bicolorSpecies of your interest not listed? Contact us
Immunogen:
Purified full length, tag cleaved, recombinant Zea mays SiR, UniProt: O23813
This antibody is also recognizing recombinant SiR protein from Zea mays.
Application Details:
assay dependent (ELISA), 1: 1000 - 1: 5000 (WB)
Purity:
Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Molecular Weight:
70 kDa (Zea mays), 72 kDa (Arabidopsis thaliana)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Sato et al. (2001). The 70-kDa major DNA-compacting protein of the chloroplast nucleoid is sulfite reductase. FEBS Lett. 2001 Jan 5;487(3):347-50.doi: 10.1016/s0014-5793(00)02342-5. (Western blot, pea)Sakibara et al. (2000). Analysis of reductant supply systems for ferredoxin-dependent sulfite reductase in photosynthetic and nonphotosynthetic organs of maize. Plant Physiol. 2000 Mar;122(3):887-94.doi: 10.1104/pp.122.3.887. (Western blot, maize)
ASN (Glutamine-dependent asparagine synthetase) is essential for nitrogen assimilation, distribution and remobilization within the plant via the phloem. ASN2 is expressed in leaf and ASN1 is expressed in floral organs. The amino acid sequences of Arabidopsis thaliana ASN1 and ASN2 are 76% identical. The amino acid sequences of Arabidopsis thaliana and Zea mays ASN2 are 73.6% identical.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 2 mg/ml.
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Zea mays
Expected Species:
Brassica rapa, Camelina sativa, Capsella rubella, Eutrema salsugineum, Punica granatumSpecies of your interest not listed? Contact us
Immunogen:
Purified full length, tag cleaved, recombinant Arabidopsis thaliana ASN2, UniProt: Q9LV77 , TAIR: AT5G65010
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Molecular Weight:
65 | 65 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Gaufichon et al. (2017). ASN1-encoded asparagine synthetase in floral organs contributes to nitrogen filling in Arabidopsis seeds. Plant J. 2017 Aug;91(3):371-393. doi: 10.1111/tpj.13567.Gaufichon et al. (2013). Arabidopsis thaliana ASN2 encoding asparagine synthetase is involved in the control of nitrogen assimilation and export during vegetative growth. Plant Cell Environ. 2013 Feb;36(2):328-42. doi: 10.1111/j.1365-3040.2012.02576.x.
Special application note:
This antibody reacts with both isoforms: ASN1 and ASN2
DHAR2 ( Dehydroascorbate Reductase 2) the protein is induced by jasmonic acid and oxidative chemical stresses and is a key component of the ascorbate recycling system. Involved in redox homeostasis under biotic and abiotic inducers. Localized in cytoplasm. Synonymes: chloride intracellular channel homolog 2, CLIC homolog 2, glutathione-dependent dehydroascorbate reductase 2, DHAR2, CytDHAR, GSH-dependent dehydroascorbate reductase 2.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Ricinus communis, Populus trichocarpa Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from known DHAR1 sequence of Arabidopsis thaliana Q9FRL8, At1g75270
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Grefen et al. (2009). The determination of protein-protein interactions by the mating-based split-ubiquitin system (mbSUS). Methods Mol Biol 479:217-233.
DHAR1 ( Dehydroascorbate Reductase 1) the protein is induced by jasmonic acid and oxidative chemical stresses and is a key component of the ascorbate recycling system. Involved in redox homeostasis under biotic and abiotic inducers. Localized in mitochondria. Synonymes: glutathione-dependent dehydroascorbate reductase 1, chloride intracellular channel homolog 1, CLIC homolog 1, glutathione-dependent dehydroascorbate reductase 1, AtDHAR1, GSH-dependent dehydroascorbate reductase 1, mtDHAR, AT1G19570.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Nicotiana tabacum, Populus trichocarpa, Ricinus communis, Solanum tuberosum, Triticum aestivum, Zinnia elegans Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from known DHAR1 sequence of Arabidopsis thaliana Q9FWR4, At1g19570
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Szymańska et al. (2019). SNF1-Related Protein Kinases SnRK2.4 and SnRK2.10 Modulate ROS Homeostasis in Plant Response to Salt Stress. Int J Mol Sci. 2019 Jan 2;20(1). pii: E143. doi: 10.3390/ijms20010143.Witzel et al. (2017). Temporal impact of the vascular wilt pathogen Verticillium dahliae on tomato root proteome. J Proteomics. 2017 Oct 3;169:215-224. doi: 10.1016/j.jprot.2017.04.008.Wang et al. (2014). Proteomic analysis of salt-responsive proteins in the leaves of mangrove Kandelia candel during short-term stress. PLoS One. 2014 Jan 8;9(1):e83141. doi: 10.1371/journal.pone.0083141. eCollection 2014.
UniProt number:
Q9FWR4
TAIR number:
AT1G19570
Research area:
Mitochondria | Respiration
Code:
Stress20
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