Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Higher plants also possess genes for significantly different, as yet uncharacterized Fd proteins, with extended C termini (FdCs). Whether these FdC proteins function as photosynthetic electron transfer proteins is not known. It has been suggested that FdC1 has a specific function in conditions of acceptor limitation at PSI, and channels electrons away from NADP(+) photoreduction.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 2 mg/ml.
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Zea mays
Expected Species:
Brassica rapa, Cannabis sativa, Theobroma cacao Species of your interest not listed? Contact us
Immunogen:
Purified full length, tag cleaved, recombinant Arabidopsis thaliana Ferredoxin C-1, UniProt: O23344 , TAIR: At4g14890
Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Molecular Weight:
16,7 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Voss et al. (2011). FdC1, a Novel Ferredoxin Protein Capable of Alternative Electron Partitioning, Increases in Conditions of Acceptor Limitation at Photosystem I. J Biol Chem. 2011 Jan 7;286(1):50-9. doi: 10.1074/jbc.M110.161562.
BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER. BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER). Alternative name: AtBP2
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4 C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Nicotiana tabacum, Oryza sativa, Physcomitrium patens, Piea sitchensis, Populus trichocarpa, Spinacia oleracea, Zea mays Species of your interest not listed? Contact us
Protein or membrane sample should be treated at 70 C for 10 min before loading on the gel
Application Details:
1 : 50-1 : 1000 (IF), 1 : 2000 (WB)
Purity:
Immunogen affinity purified total IgY. in PBS pH 7.4.
Molecular Weight:
73,5 | 80 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Bennett et al. (2014). Plasma Membrane-Targeted PIN Proteins Drive Shoot Development in a Moss. Curr Biol. 2014 Dec 1;24(23):2776-85. doi: 10.1016/j.cub.2014.09.054. Epub 2014 Nov 13.
Special application note:
Antibody solution contains 0,02% sodium azide as preservative
Glutamine oxoglutarate aminotransferase (GOGAT) is an enzyme involved in synthesis of glutamate from glutamine and alpha-ketoglutarate. GOGAT has two forms in plants: ferredoxin-dependent GOGAT (Fd-GOGAT) and NADH-dependent GOGAT (NADH-GOGAT). 95% of GOGAT found in plants is the Fd-GOGAT type. Fd-GOGAT is encoded by two genes, glu1 and glu2 in Arabidopsis. Fd-GOGAT (both forms) is highly conserved among plants, red algae, and cyanobacteria. Ferredoxin-dependent glutamate synthase, chloroplastic (Fd-GOGAT) is involved in glutamate biosynthesis in leaf. This protein required for the reassimilation of ammonium ions generated during photorespiration. Gene name is GlsF.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 2 mg/ml.
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Ariga and Hase (2014). Multiple complexes of nitrogen assimilatory enzymes in spinach chloroplasts: possible mechanisms for the regulation of enzyme function. PLoS One. Oct 1;9(10):e108965. doi: 10.1371/journal.pone.0108965.Sakaibara et al. (1991). Molecular cloning and characterization of complementary DNA encoding for ferredoxin-dependent glutamate synthase in maize leaf. J Biol Chem. Feb 5;266(4):2028-35.
Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Occupies a key position both for transferring the photoreducing power to Fd-NADP+ oxidoreductase (FNR), hence the formation of NADPH, and for mediating the cyclic electron flow around photosystem I (PSI). Fd2 is most abundant Fd isoprotein expressed in plant leaves.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 2 mg/ml.
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Molecular Weight:
15 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Ramirez et al. (2013). Glutathione and ascorbic acid protect Arabidopsis plants against detrimental effects of iron deficiency.Hanke et al. (2004). A post genomic characterizationof Arabidopsis ferredoxins. Plant Physiol. 2004 Jan;134(1):255-64. Epub 2003 Dec 18 (Western blot, Arabidopsis thaliana)
Cyclin-B1-1 is a cyclin-dependent protein kinase involved in cell division. Functions as an effector of growth contol at G2/M. Alternative names: Cyc1-At, G2/mitotic-specific cyclin-B1-1, CycB1;1, CYCB1-1, CYC1
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C or -80 C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica campestris, Capsella rubella, Eutrema salsugineum, Noccaea caerulescensSpecies of your interest not listed? Contact us
Immunogen:
Recombinant Cyclin-B1-1 protein derived from Arabidopsis thaliana sequence, amino acids: 1-428. UniProt: P30183 TAIR: AT4G37490
>95%, Protein G purified to a total immunoglobulin G fraction.
Molecular Weight:
48,4 | 49 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Preservative: 0.03% Proclin 300. Preparation contains: 50% Glycerol, 10 mM PBS, pH 7.4Reactivity of this antibody on endogenous extract remains to be confirmed.
MEB1 (Membrane protein of ER body 1) displays iron ion transmembrane transporter activity and may sequester excess cytosolic iron and manganese into endoplasmic reticulum to reduce metal ion toxicity. Not essential for the accumulation of ER body components.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 2 mg/ml.
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
Purified recombinant MEB1 of Arabidopsis thaliana, residues 271-502 with a His tag, UniProt: Q8W4P8, TAIR: AT4G27860
Applications:
ELISA (ELISA), Immunoprecipitation (IP), Western blot (WB)
Tubulin alpha (TUA) together with beta tubulin is making up microtubules.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at 4 C; Do not freeze. Do not exceed exipry date is provided on the tube. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
This antibody is recognizing defined epitope (amino acid 65-97) on N-terminal structural domain of alpha tubulin.Recommended secondary antibody, goat anti-mouse IgG1, HRP conjugated AS16 3715
Application Details:
1 : 500 (ICC), 1-4 g/ml /FlowCyt), 1-4 g/ml (WB)
Conjugation:
IgG1
Isotype:
IgG1
Purity:
Immunoglobulin Protein A purified in PBS. Contain 15 mM sodium azide.
Molecular Weight:
51 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Liu et al. (2022) Identification of positive and negative regulators of antiviral RNA interference in Arabidopsis thaliana. Nat Commun. 2022 May 30;13(1):2994. doi: 10.1038/s41467-022-30771-0. PMID: 35637208; PMCID: PMC9151786.
Special application note:
Metal induced stress affected the expression of tubulin, and that therefore, this protein cannot be used as a loading control under that type of conditions, data in application example,
Glutamine synthetase plays a role in the flow of nitrogen into nitrogenous organic compounds. There are five root types of GS isoproteis (GS1-1~ GS1-5) and one chloroplastic GS isoprotein (GS2/GLN2) are known. The sequence identity between GS1-1 and GS2 is 65%.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 2 mg/ml.
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Spinacia oleracea, Zea mays
Expected Species:
Brachypodium distachyon, Cucurbita moschata, Glycine max, Medicago truncatula, Oryza sativa, Panicum hallii, Pontederia crassipes, Populus tremula x Populus alba, Prunus persica, Raphanus sativus, Saccharum officinarum, Setaria italica, Sorghum bicolor, Triticum aestivumSpecies of your interest not listed? Contact us
Immunogen:
Purified full length, tag cleaved, recombinant Zea mays GS-1 (Glutamine synthetase, root isozyme 1) UniProt: P38559
No confirmed exceptions from predicted reactivity are currently known
Application Details:
assay dependent (ELISA), 1: 1000 - 1: 5000 (WB)
Purity:
Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Molecular Weight:
39 kDa | 43 kDa (chloroplast isoform)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Kimata-Ariga and Hase (2014). Multiple complexes of nitrogen assimilatory enzymes in spinach chloroplasts: possible mechanisms for the regulation of enzyme function. PLoS One . 2014 Oct 1;9(10):e108965. doi: 10.1371/journal.pone.0108965. Sakaibara et al. (1996). Molecular identification and characterization of cytosolic isoforms of glutamine synthetase in maize roots. J Biol Chem. 1996 Nov 22;271(47):29561-8. doi: 10.1074/jbc.271.47.29561.
Special application note:
This antibody reactis with all glutamine synthetase isotypes from leaf and root
The major light-harvesting antenna complex II (LHCII) in photosynthetic eukaryotes is located in the thylakoid membrane of the chloroplast. It is a heterotrimeric complex formed by up to 3 different individual subtypes of chlorophyll a/b-binding proteins: Lhcb1, Lhcb2, and Lhcb3. Lhcb1 is the most abundant chlorophyll a/b-binding protein in eukaryotic phototrophs and often is coded by several nuclear genes.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Digitaria sanguinalis, Echinochloa crus-galli, Pinus strobus L.
Expected Species:
Algae, Dicots, Mosses Species of your interest not listed? Contact us
Immunogen:
BSA-conjugated synthetic peptide derived from Arabidopsis thaliana At1g29910 (Lhcb1.1), At1g29920 (Lhcb1.2), At1g29930 (Lhcb1.3, most expressed), At2g34430 (Lhcb1.4), and At2g34420 (Lhcb1.5)
This Lhcb1 antibody is directed specifically against the Arabidopsis Lhcb1 gene products, for those that would prefer higher specific activity over broader specificity offered by Agrisera older Lhcb1 antibody, AS01 004Protein is processed into mature form (Jansson 1999).
Application Details:
1 : 2500-1 : 5000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 100 µl of sterile water
Molecular Weight:
28 | 25 kDa (Arabidopsis thaliana)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Wang et al. (2020). Post-translational coordination of chlorophyll biosynthesis and breakdown by BCMs maintains chlorophyll homeostasis during leaf development. Nat Commun. 2020; 11: 1254. Pralon et al. (2019). Plastoquinone homoeostasis by Arabidopsis proton gradient regulation 6 is essential for photosynthetic efficiency. Commun Biol. 2019 Jun 20;2:220. doi: 10.1038/s42003-019-0477-4. Lal et al. (2018). The Receptor-like Cytoplasmic Kinase BIK1 Localizes to the Nucleus and Regulates Defense Hormone Expression during Plant Innate Immunity. Cell Host Microbe. 2018 Apr 11;23(4):485-497.e5. doi: 10.1016/j.chom.2018.03.010.Tamburino et al. (2017). Chloroplast proteome response to drought stress and recovery in tomato (Solanum lycopersicum L.). BMC Plant Biol. 2017 Feb 10;17(1):40. doi: 10.1186/s12870-017-0971-0.Fristedt et al. (2017). PSB33 sustains photosystem II D1 protein under fluctuating light conditions. Journal of Experimental Botany doi:10.1093/jxb/erx218.Hartings et al. (2017). The DnaJ-Like Zinc-Finger Protein HCF222 Is Required for Thylakoid Membrane Biogenesis in Plants. Plant Physiol. 2017 Jul;174(3):1807-1824. doi: 10.1104/pp.17.00401.Correa-Galvis et al. (2016). PsbS interactions involved in the activation of energy dissipation in Arabidopsis. Nature Plants 2, Article number: 15225 (2016) doi:10.1038/nplants.2015.225Longoni et al. (2015). Phosphorylation of the Lhcb2 isoform of Light Harvesting Complex II is central to state transitions. Plant Physiol. 2015 Oct 5. pii: pp.01498.2015.Wientjes et al (2013). LHCII is an antenna of both photosystems after long-term acclimation. BBA, Jan 6.
Special application note:
A molecular characterisation of the LHCII proteins can be found in Caffarri et al. (2004) A Look within LHCII: Differential Analysis of the Lhcb1−3 Complexes Building the Major Trimeric Antenna Complex of Higher-Plant Photosynthesis. Biochemistry 43 (29): 9467–9476
Degradation of the most abundant membrane protein on earth, the light-harvesting complex of Photosystem II (LHC II), is highly regulated under various environmental conditions, e.g., light stress, to prevent photochemical damage to the reaction center. FtsH6 is proposed to be a general LHC II protease and FtsH6-dependent LHC II proteolysis can be a feature of all higher plants.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide chosen from AtFtsH6 protein sequence of Arabidopsis thaliana Q1PDW5
For reconstitution add 100 µl of sterile water/tube
Molecular Weight:
74,36 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Sedaghatmehr et al. (2022) Heat shock factor HSFA2 fine-tunes resetting of thermomemory via plastidic metalloprotease FtsH6. J Exp Bot. 2022 Jun 15:erac257. doi: 10.1093/jxb/erac257. Epub ahead of print. PMID: 35705109.Sedaghatmehr et al. (2016). The plastid metalloprotease FtsH6 and small heat shock protein HSP21 jointly regulate thermomemory in Arabidopsis. Nat Commun. 2016 Aug 26;7:12439. doi: 10.1038/ncomms12439.
SMT1 (EC=2.1.1.41) is an enzyme involved in steroid biosynthesis localized specific to the Endoplasmic Reticulum (ER), Boutte et al., 2009 . It is catalyzing the methyl transfer from S-adenosyl-methionine to the C-24 of cycloartenol to form 24-methylene cycloartenol. The protein is highly expressed in vascular tissue, mature leaves and in regions undergoing cellular expansion. Alternative names: Cycloartenol-C-24-methyltransferase, 24-sterol C-methyltransferase 1, Protein STEROL METHYLTRANSFERASE 1, Protein CEPHALOPOD
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Amborella trichopoda, Brassica napus, Brassica rapa, Capsella rubella, Citrus clementina, Eutrema salsugineum, Glycine max, Glycine soja, Gossypium mexicanum, Medicago truncatula, Populus trichopocarpa, Prunus persica, Ricinus communis, Theobroma cacao, Vitis vinifera Species of your interest not listed? Contact us
SMT1 antibody characterization in Western Blot and immunofluorescence labeling: Boutt Y et al. (2010). Endocytosis restricts Arabidopsis KNOLLE syntaxin to the cell division plane during late cytokinesis. EMBO J. 29, 5465-5458.
Ming-fang et al. (2021) Improved quantification of immune-gold labeling and its use to compare the distribution of cellular factors among sub-chloroplast compartments,Micron,2021,103060,ISSN 0968-4328,https://doi.org/10.1016/j.micron.2021.103060.Cano-Ramirez et al. (2021) M. Plasma Membrane Fluidity: An Environment Thermal Detector in Plants. Cells. 2021 Oct 17;10(10):2778. doi: 10.3390/cells10102778. PMID: 34685758; PMCID: PMC8535034.Collins et al. (2020). EPSIN1 Modulates the Plasma Membrane Abundance of FLAGELLIN SENSING2 for Effective Immune Responses . Plant Physiol. 2020 Feb 24. pii: pp.01172.2019. doi: 10.1104/pp.19.01172Laohavisit (2020). Quinone perception in plants via leucine-rich-repeat receptor-like kinases. Nature. 2020 Nov;587(7832):92-97. doi: 10.1038/s41586-020-2655-4. Epub 2020 Sep 2. PMID: 32879491.Yang et al. (2016). Arabidopsis PROTEASOME REGULATOR1 is required for auxin-mediated suppression of proteasome activity and regulates auxin signalling. Nat Commun. 2016 Apr 25;7:11388. doi: 10.1038/ncomms11388.
Special application note:
SMT1 is an integral membrane protein of ER (Boutte et al., 2009) while BiP is a membrane associated protein.Endogenous SMT1 is expressed at very low levels in root epidermis and root cap as compare to cortex and endodermis and therefore this can contribute to SMT1 detection problems.
Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Occupies a key position both for transferring the photoreducing power to Fd-NADP+ oxidoreductase (FNR), hence the formation of NADPH, and for mediating the cyclic electron flow around photosystem I (PSI).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 2 mg/ml.
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Zea mays
Expected Species:
plant ferredoxin isoformsSpecies of your interest not listed? Contact us
Immunogen:
Native, chromafography purified ferredoxin mixture: Fd1, Fd2, Fd3 and Fd4 from Zea mays, UniProt: P27787 (Fd1), P27787(Fd2), P27788 (Fd3), accesion number not known for Fd4
Following processing MW of plant ferredoxins is around 12 kDa, However, due to a strong acidic nature, they migrate at 16 to 17 kDa
Application Details:
1: 1000 - 1: 10 000 (WB)
Purity:
Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Molecular Weight:
12 | 16-17 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Hase et al. (1991). Molecular Cloning and Differential Expression of the Maize Ferredoxin Gene Family. Plant Physiol. 96(1):77-83. doi: 10.1104/pp.96.1.77.
Aleu protein may play a role in proteolysis leading to mobilization of nitrogen during senescence and starvation. Catalytic activity : Hydrolysis of proteins, acting as an aminopeptidase (notably, cleaving Arg-|-Xaa bonds) as well as an endopeptidase. Cellular localisation: vacuole. Alternative names: Senescence-associated gene product 2
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 2 mg/ml.
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica oleracea, Camelina sativa, Capsella rubella, Eutrema salsugineum, Raphanus sativus Species of your interest not listed? Contact us
Immunogen:
Recombinant, His6-tagged, ALEU protein from Arabidopsis thaliana, UniProt: Q8H166, TAIR: At5g60360
Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Molecular Weight:
38,9 | 24 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Takagi et al. et al. (2013). MAIGO5 functions in protein export from Golgi-associated endoplasmic reticulum exit sites in Arabidopsis. Plant Cell. 2013 Nov;25(11):4658-75. doi: 10.1105/tpc.113.118158. (Western blot, Arabidopsis thaliana)Ueda et al. (2006). AtVAM3 is required for normal specification of idioblasts, myrosin cells. Plant Cell Physiol. 2006 Jan;47(1):164-75. doi: 10.1093/pcp/pci232. (Western blot, Arabidopsis thaliana)
HA-tag is derived from a human influenza hemagglutinin HA-molecule corresponding to amino acids 96-106 and is used as a general epitope tag in expression vectors.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Important note: there is a strong cross reactivity between 25 -20 kDa in Arabidopsis thaliana wildtype.
Application Details:
1 : 5000 (WB)
Purity:
affinity purified serum in PBS, pH 7.4.
Reconstitution:
For reconstitution add 100 µl of sterile water
Molecular Weight:
Depends on the MW of the protein which is HA-tagged
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Hwang et al. (2019) Arabidopsis ABF3 and ABF4 Transcription Factors Act with the NF-YC Complex to Regulate SOC1 Expression and Mediate Drought-Accelerated Flowering. Mol Plant. 2019 Apr 1;12(4):489-505. doi: 10.1016/j.molp.2019.01.002
1-aminocyclopropane-1-carboxylate synthase (ACS) enzymes catalyze the conversion of S-adenosyl-L-methionine (SAM) into 1-aminocyclopropane-1-carboxylate (ACC), a direct precursor of ethylene. (EC:4.4.1.14). Synonymes: S-adenosyl-L-methionine methylthioadenosine-lyase 7.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
PYK10 is the main component of ER bodies. It has hydrolase activity, hydrolyzing O-glycosyl compounds It may produce defense compounds when plants are damaged by insects or wounding. Cellular localisation: ER bodies.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 2 mg/ml.
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
Conjugated peptide, derived from Arabidopsis thaliana C-terminal of PYK10, UniProt: A0A178VCN3, TAIR: At3g08880.
N-terminal signal peptide including 24 amino acis and ER retention signal is removed from the mature protein
Application Details:
1:500-1:1000 (IHC), 1: 5000- 1: 20 000 (WB)
Purity:
Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Molecular Weight:
59,7 | 56 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Matsushima et al. (2003). A novel ER-derived compartment, the ER body, selectively accumulates a beta-glucosidase with an ER-retention signal in Arabidopsis. Plant J. 2003 Feb;33(3):493-502. doi: 10.1046/j.1365-313x.2003.01636.x.
DEG15 is a peroxisomal Deg-protease with endopeptidase activity. This protease cleaves specifically substrates with Cys in the P1 and P2 position and acts as peroxisomal processing peptidase. Alternative names: At1g28320/F3H9_2
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Micromonas sp., Oryza sativa, Populus balsamifera, Solanum lycopersicum, Sorghum vulgare, Ricinus communis, Vitis vinifera, Zea mays Species of your interest not listed? Contact us
Immunogen:
synthetic peptide derived from Arabidopsis thaliana DEG15, Q8VZD4 (At1g28320)
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Schuhmann et al. (2008). The DEG15 serine protease cleaves peroxisomal targeting signal 2-containing proteins in Arabidopsis.Plant Physiol. 4: 1847-1856.Helm et al. (2007). Dual specificities of the glyoxysomal/peroxisomal processing protease Deg15 in higher plants.PNAS 27: 11501-11506.
UCP (uncoupling protein) is an inner membrane mitochondrial protein that can dissipate the proton gradien before it can be used to provide the energy for oxidative phosphorylation. Synonymes: AtUCP, Uncoupling protein 2.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Czobor et al. (2019). Comparison of the response of alternative oxidase and uncoupling proteins to bacterial elicitor induced oxidative burst. PLoS One. 2019 Jan 10;14(1):e0210592. doi: 10.1371/journal.pone.0210592.Tak č et al. (2018). Shot-Gun Proteomic Analysis on Roots of Arabidopsis pldα1 Mutants Suggesting the Involvement of PLDα1 in Mitochondrial Protein Import, Vesicular Trafficking and Glucosinolate Biosynthesis. Int J Mol Sci. 2018 Dec 26;20(1). pii: E82. doi: 10.3390/ijms20010082. (immunolocalization)Garmash et al. (2017). Expression profiles of genes for mitochondrial respiratory energy-dissipating systems and antioxidant enzymes in wheat leaves during de-etiolation. J Plant Physiol. 2017 Aug;215:110-121. doi: 10.1016/j.jplph.2017.05.023.Florez-Sarasa et al. (2016). Impaired cyclic electron flow around Photosystem I disturbs high-light respiratory metabolism. Plant Physiol. 2016 Oct 19. pii: pp.01025.2016.Liu et al. (2015). Silencing of mitochondrial uncoupling protein gene aggravates chilling stress by altering mitochondrial respiration and electron transport in tomato. Acta Physiologiae Plantarum November 2015, 37:223.Long et al. (2015). Contributions of photosynthetic and non-photosynthetic cell types to leaf respiration in Vicia faba L. and their responses to growth temperature. Plant Cell Environ. 2015 Apr 1. doi: 10.1111/pce.12544.Grabelnych et al. (2014). Mitochondrial Energy Dissipating Systems (Alternative Oxidase, Uncoupling Proteins, and External NADH Dehydrogenase) Are Involved in Development of Frost Resistance of Winter Wheat Seedlings. ISSN 0006 2979, Biochemistry (Moscow), 2014, Vol. 79, No. 6, pp. 506 519. Pleiades Publishing, Ltd., 2014.Barreto et al. (2014). Overexpression of UCP1 in tobacco induces mitochondrial biogenesis and amplifies a broad stress response. BMC Plant Biol. 2014 May 28;14(1):144.
Special application note:
Peptide used to elicit this antibody is conserved in both isoforms, UCP1 and UCP2 of Arabidopsis thaliana.
PYK10 is the main component of ER bodies. It has hydrolase activity, hydrolyzing O-glycosyl compounds It may produce defense compounds when plants are damaged by insects or wounding.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 2 mg/ml.
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
Conjugated peptide, derived from Arabidopsis thaliana internal part of PYK10, UniProt: A0A178VCN3, TAIR: At3g08880.
Applications:
Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB)
Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Molecular Weight:
59,7 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Yamada et al. (2013). Identification of Two Novel Endoplasmic Reticulum Body-Specific Integral Membrane Proteins. Plant Physiol. 2013 Jan;161(1):108-20. doi: 10.1104/pp.112.207654. (Western blot, Arabidopsis thaliana) Nagano et al. (2005). Activation of an ER-body-localized -Glucosidase via a Cytosolic Binding Partner in Damaged Tissues of Arabidopsis thaliana. Plant Cell Physiol. 2005 Jul;46(7):1140-8. doi: 10.1093/pcp/pci126. (Immunoprecipiation, Western blot, Arabidopsis thaliana)Matshushima et al. (2003). A novel ER-derived compartment, the ER body, selectively accumulates a beta-glucosidase with an ER-retention signal in Arabidopsis. Plant J . 2003 Feb;33(3):493-502. doi: 10.1046/j.1365-313x.2003.01636.x. Immunofluorescence, Immunohistochemistry, Western blot, Arabidopsis thaliana)
Delta VPE (Vacuplar-Processing Enzyme delta-isozyme) is Asparagine specific endopeptidase that may be involved in processing of proteins targeted to vacuoles (By similarity). The enzyme is probably involved in post-translational proteolysis of seed storage proteins in the protein storage vacuole of developing seeds (PubMed:12417707, PubMed:14688293). Exhibits a caspase-1-like activity in extracellular granules. At the early stage of seed development, required for the formation of the seed coat, by regulating cell death of specific cell layers in inner integument (Nakaune et al. 2005).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 2 mg/ml.
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Camelina sativa, Capsella rubella, Eutrema salsugineum, Raphanus sativus Species of your interest not listed? Contact us
Immunogen:
Recombinant, His6-tagged, delta-VPE from Arabidopsis thaliana, UniProt: Q9LJX8, TAIR: At3g20210
Applications:
Immunofluorescence (IF), Immunolocalisation (IL), Western blot (WB)
Subcellular localisation is seed specific and restricted to developing seeds at 7 days after anthesis, Delta-VPE is detected at lower levels in flowers siliques, specifically in seed coats
Application Details:
1: 500 (IF), 1: 5000 (IL), 1: 5000 (WB)
Purity:
Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Molecular Weight:
52 kDa (inactive precursor) | 37-38 kDa (mature, active form)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Kunieda et al. (2013). Spatiotemporal secretion of PEROXIDASE36 is required for seed coat mucilage extrusion in Arabidopsis. Plant Cell . 2013 Apr;25(4):1355-67.doi: 10.1105/tpc.113.110072. (Western blot, Arabidopsis thaliana)Kunieda et al. (2008). NAC family proteins NARS1/NAC2 and NARS2/NAM in the outer integument regulate embryogenesis in Arabidopsis. Plant Cell. 2008 Oct;20(10):2631-42.doi: 10.1105/tpc.108.060160. (Western blot, Arabidopsis thaliana)Nakaune et al. (2005). A vacuolar processing enzyme, deltaVPE, is involved in seed coat formation at the early stage of seed development. Plant Cell. 2005 Mar;17(3):876-87. doi: 10.1105/tpc.104.026872. (Immunofluorescence, Immunolocalisation by electron microscopy, Western blot, Arabidopsis thaliana)
Tubulin beta (TUB) together with alpha tubulin is making up microtubules.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Metal induced stress affected the expression of tubulin, and that therefore, this protein cannot be used as a loading control under that type of conditions. More information can be found here.
Application Details:
1 : 1000 (IF), 1 : 500 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 100 µl of sterile water
Molecular Weight:
49 | 49 kDa (Arabidopsis thaliana)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Durian et al. (2019). PROTEIN PHOSPHATASE 2A-B' gamma controls Botrytis cinerea resistance and developmental leaf senescence. Plant Physiol. 2019 Oct 28. pii: pp.00893.2019. doi: 10.1104/pp.19.00893.Wang et al. (2017). The inhibition of protein translation mediated by AtGCN1 is essential for cold tolerance in Arabidopsis thaliana. Plant Cell Environ. 2017 Jan;40(1):56-68. doi: 10.1111/pce.12826.Heinnickel et al. (2016). Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly. Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):2774-9. doi: 10.1073/pnas.1524040113
Special application note:
Please, note that tubulin beta is only detected in actively dividing meristematic cells (see immunolocalization image below). Therefore to allow detection on a western blot, analyzed material must contain enough meristematic cells with dividing activity.Signal in a western blot application in Chlamydomonas reinhardtii is obtained with a load of 100 g/well and a dilution of 1: 500.
NPR1 (regulatory protein NPR1) is a key positive regulator of the SA-dependent signaling pathway that negatively regulates JA-dependent signaling pathway. Controls the onset of systemic acquired resistance (SAR). Subcellular localization: cytoplasm, nucleaus (following induction by salicylic acid treatment or after pathogen infection. Alternative names: BTB/POZ domain-containing protein NPR1, Non-inducible immunity protein 1, Nim1, Salicylic acid insensitive 1, Sai1, ATNPR1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide, chosen from NPR1 sequence of Arabidopsis thaliana, TAIR: AT1G64280, UniProt: P93002
For successful detection using NPR1 antibody please follow protocol suggested below. NPR1 protein readily oligomerizes, in addition to any naturally occurring oligomer, during extraction. Therefore 50 mM DTT has to be used as well as denaturation at 75 C for 15 minutes. Engogenous NPR1 level is very low, thereofre SA treatment is absolutely necessary for good detection.This antibody is recognizing NPR1-GFP in the 35S overexpression line.
Goat anti-rabbit IgG (H&L), F(ab)'2 fragment is a secondary antibody conjugated to HRP which binds to rabbit IgG (H&L) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Rabbit IgG (H&L)
Expected Species:
Rabbit IgG (H&L)
Immunogen:
Purified Rabbit IgG (H&L)
Applications:
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Linster et al. (2015). Downregulation of N-terminal acetylation triggers ABA-mediated drought responses in Arabidopsis. Nat Commun. 2015 Jul 17;6:7640. doi: 10.1038/ncomms8640.
Special application note:
Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.0)HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidasePurity is ≥ 90% based on SDS-PAGE. May contain small amounts of intact IgG.
The light-harvesting protein Lhca1 is one of the four main and highly conserved types of chlorophyll a/b-binding proteins (Lhca1-4) of the light harvesting antenna (LHCI) of plant photosystem I. Lhca1 is imported as a precursor from the cytosol into the chloroplast. Upon insertion into the thylakoid membrane Lhca1 forms a heterodimer (LHCI-730) with Lhca4 that associates with the PSI core close to PsaG and PsaF.A biochemical characterization of the plant LHCI antenna can be found in Klimmek et al. (2005) The structure of the higher plant light harvesting complex I: in vivo characterization and structural interdependence of the Lhca proteins. Biochemistry 44: 3065–3073
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
BSA-conjugated synthetic peptide derived from the Lhca1 protein of Arabidopsis thaliana UniProt: Q01667, TAIR: At3g54890. This sequence is highly conserved in Lhca1 proteins of angiosperms (monocots and dicots) and gymnosperms.
Protein is processed into mature form (Jansson 1999).
Application Details:
1 : 2000-1 : 5000 (WB)
Purity:
Total IgG. Protein G purified in PBS pH 7.4.
Reconstitution:
For reconstitution add 100 l of sterile water
Molecular Weight:
25.99 | 22 kDa for Arabidopsis thaliana
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Harchouni et al. (2022) Guanosine tetraphosphate (ppGpp) accumulation inhibits chloroplast gene expression and promotes super grana formation in the moss Physcomitrium (Physcomitrella) patens. New Phytol. 2022;236(1):86-98. doi:10.1111/nph.18320Espinoza-Corral & Lundquist. (2022) The plastoglobule-localized protein AtABC1K6 is a Mn2+-dependent kinase necessary for timely transition to reproductive growth. J Biol Chem. 2022 Apr;298(4):101762. doi: 10.1016/j.jbc.2022.101762. Epub 2022 Feb 22. PMID: 35202657; PMCID: PMC8956952.Sarvari et al. (2022). Qualitative and quantitative evaluation of thylakoid complexes separated by Blue Native PAGE. Plant Methods. 2022 Mar 3;18(1):23. doi: 10.1186/s13007-022-00858-2. PMID: 35241118; PMCID: PMC8895881.Xiong et al. (2022) a chloroplast nucleoid protein of bacterial origin linking chloroplast transcriptional and translational machineries, is required for proper chloroplast gene expression in Arabidopsis thaliana. Nucleic Acids Res. 2022 Jun 23;50(12):6715-34. doi: 10.1093/nar/gkac501. Epub ahead of print. PMID: 35736138; PMCID: PMC9262611.Kumari et al. (2021) In-depth assembly of organ and development dissected Picrorhiza kurroa proteome map using mass spectrometry. BMC Plant Biol. 2021 Dec 22;21(1):604. doi: 10.1186/s12870-021-03394-8. PMID: 34937558; PMCID: PMC8693493.
Special application note:
Antibody format is a total IgG fraction, which means that it is a pool of polyclonal antibodies obtained by purification of serum on Protein G, not on a specific antigen column.This product can be sold containing ProClin if requested.
SUMO1 - Small Ubiquitin-like Modifier ubiquitin like protein binds in reversible way to various protein targets and plays a role as a signaling regulator.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Glycine max, Nicotiana tabacum, Picea sitchensis, Pisum sativum, Populus trichocarpa, Solanum lycopersicum, Zea maysSpecies of your interest not listed? Contact us
Immunogen:
Recombinant proSUMO1 from Arabidopsis thaliana Q547B9, At4g26840 with a his tag
Antibodies will also detect SUMO2 protein. Suggested extraction buffer: 100 mM Tris-HCl, pH 8.0, 0.1% [w/v] SDS, 0.5% [w/v] sodium deoxycholate, 1% [v/v] glycerol, 50 mM sodium metabisulfite, 20 mM N-ethylmaleimide (NEM) and protease inhibitor cocktail (Roche) (Orosa et al. 2018). This buffer will help to stabilize the conjugates and will help to detect any increase or decrease in conjugate accumulation using the antibodies.
Application Details:
1 : 1000-1 : 5000 (WB)
Purity:
Total IgG. Protein G purified in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
10,97 | 12 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Szadeczky-Kardoss et al. (2022) Elongation factor TFIIS is essential for heat stress adaptation in plants. Nucleic Acids Res. 2022 Feb 28;50(4):1927-1950. doi: 10.1093/nar/gkac020. PMID: 35100405; PMCID: PMC8886746.Colignon et al. (2019). Dual coordination of the SUMOylation and phosphorylation pathways during the response to heat stress in Solanum tuberosum. Environmental and Experimental Botany Volume 162, June 2019, Pages 192-200.Rosa et al. (2018). Insights into the transcriptional and post-transcriptional regulation of the rice SUMOylation machinery and into the role of two rice SUMO proteases. BMC Plant Biol. 2018 Dec 12;18(1):349. doi: 10.1186/s12870-018-1547-3.Guo et al. (2017). Sumoylation stabilizes RACK1B and enhance its interaction with RAP2.6 in the abscisic acid response. Sci Rep. 2017 Mar 8;7:44090. doi: 10.1038/srep44090.Tomanov et al. (2014). Arabidopsis PIAL1 and 2 Promote SUMO Chain Formation as E4-Type SUMO Ligases and Are Involved in Stress Responses and Sulfur Metabolism. Plant Cell. 2014 Nov;26(11):4547-60. doi: 10.1105/tpc.114.131300.
UniProt number:
Q547B9
TAIR number:
AT4G26840
Research area:
Arabidopsis thaliana antibodies
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