Cluster of Differentiation 5 (CD5) is expressed in high levels on the surface of T-cells, while the expression levels and role of CD5 in B-cells is not well documented. As a part of a diagnostic panel, its utility lies predominantly as a marker for T-cells, with over 70% of T-cell neoplasms expressing CD5. In particular, it is correlated with chronic lymphocytic leukemia and small lymphocytic lymphomas, mantle cell lymphoma, as well as a subset of diffuse large B-cell lymphomas. CD5 demonstrates positive expression in thymic carcinomas, and is not as sensitive as CD3. CD5 also has value as a prognostic indicator, as it is associated with poor prognosis in acute T-cell lymphoblastic leukemia.
B7H4 is a glycosylated transmembrane protein of the B7 family. It binds to activated T cells to moderate the T cell responses via cell cycle arrest in the T cell. Reverse signaling can induce either cell cycle arrest or apoptosis in the B7H4 expressing cell. B7H4 is up-regulated in several carcinomas in correlation with tumor progression and metastasis. A soluble form of B7H4 is elevated in the serum of ovarian cancer, renal cell carcinoma, and rheumatoid arthritis patients, also in correlation with advanced disease status.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC039
Antibody Isotype:
IgG2b
GMDN Code:
?
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Breast
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
ATRX is involved in the remodeling of the nucelosome structure, and facilitate the transcription and replication. ATRX loss occurs in grades II/III astrocytomas and glioblastomas and comes together with IDH1 mutations. Detection of ATRX with IHC acts a sensitive method to identify the mutations.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC703
Antibody Isotype:
IgG2b
GMDN Code:
65252
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Breast
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Arginase-1, encoded by the ARG1 gene, is a cytosolic metalloenzyme expressed predominantly in hepatocytes. Arginase-1 plays a key role in the urea cycle by catalyzing the hydrolysis of arginine to ornithine and urea. Argininemia is an inherited autosomal recessive disorder characterized by a buildup of arginine and ammonia in the blood. Anti-Arginase-1 is highly specific for hepatocytes, and is therefore a sensitive and specific marker of benign and malignant hepatic tumours.
Annexin A1 (ANXA1) is a membrane protein that plays a role in innate and adaptive immunity by controlling the biosynthesis of inflammation, prostaglandins, and leukotriene mediators. This target is overexpressed in 97% of all samples from patients with hairy cell leukemia, and is absent in other B-cell lymphomas. High ANXA1 expression is frequently associated with advanced stage esophageal and esophagogastric junction adenocarcinoma, and is also linked to advanced and metastatic disease states.
Androgen Receptor (AR) is a transcriptional regulator with a broad array of functions. This marker is clinically significant in the understanding of tumour progression and tumour aggressiveness. The detection of AR by immunohistochemical staining is important for diagnosis of all types of prostate carcinoma, including both therapy-responsive and therapy-unresponsive disease states. Co-testing with AR and CK20 is used for differential diagnosis of desmoplastic trichoepithelioma (DTE) [CK20+/AR-], morpheaform basal cell carcinoma (BCC) [CK20-/AR+], and microcystic adnexal carcinoma (MAC) [CK20-/AR-].
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC511
Antibody Isotype:
IgG
GMDN Code:
56796
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Prostate Carcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Alpha-Fetoprotein (AFP) is a major plasma glycoprotein seen in hepatocytes of fetal liver and in hepatoma. Elevated levels of AFP in adult serum may be indicative of hepatocellular carcinoma, hepatoid adenocarcinoma, germ cell tumours, or yolk sac tumours. In hepatocellular carcinoma, AFP expression usually indicates malignancy in a hepatocellular nodule and hepatic histogenesis of a malignancy.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC714
Antibody Isotype:
IgG
GMDN Code:
56770
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Hepatocellular Carcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Anaplastic Lymphoma Kinase (ALK) is a receptor tyrosine kinase which plays a role in brain and nervous system development. ALK is typically expressed at low levels in regions of the developing central and peripheral nervous system, such as the neonatal brain and spinal cord. The most common genetic alterations of this gene are chromosomal translocations, which result in multiple ALK fusion proteins that are involved in tumourigenesis, as in the case of anaplastic large cell lymphoma (ALCL), lung adenocarcinoma, and inflammatory myofibroblastic tumours. Aberrant ALK expression is also found in other tumours such as familial neuroblastoma, non-small cell lung carcinoma (NSCLC), and brain cancers.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC509
Antibody Isotype:
IgG2b
GMDN Code:
56791
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Anaplastic Large Cell Lymphoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Aldo-Keto Reductase Family 1 Member B10 (AKR1B10) is an enzyme of the aldo-keto reductase superfamily, and catalyzes the reduction of aliphatic and aromatic aldehydes. AKR1B10 is commonly expressed in adrenal glands, the small intestine, and colon tissues. AKR1B10 staining is useful in the recognition of liver carcinogenesis.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC508
Antibody Isotype:
IgG2a
GMDN Code:
?
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Hepatocellular Carcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Actin is part of the cytoskeletal system of all cell types. Smooth muscle actin is found in myofibroblasts and myoepithelium, but not in cardiac or skeletal muscles. Labeling of smooth muscle actin in concert with muscle specific actin staining can allow for differentiation between rhabdomyosarcoma and leiomyosarcoma, as muscle-specific actin is found in rhabdomyoblasts, while smooth muscle actin is found in leiomyosarcomas.
Actin is part of the cytoskeletal system of every cell type. It can be classified based on isoelectric points as alpha, beta, and gamma. Muscle Specific Actin includes those of the alpha and gamma isotypes. Skeletal, smooth, and cardiac muscle cells will all stain positively with Anti-Muscle Specific Actin, but mesenchymal cells, not including myoepithelium, will stain negatively. Normal and neoplastic non-muscle cells, including vascular endothelial and connective tissues, carcinomas, melanomas, and lymphomas, will also be negative for muscle specific actin. The use of Anti-Muscle Specific Actin in concert with Anti-Smooth Muscle Actin can allow for differentiation between rhabdomyosarcoma and leiomyosarcoma, as muscle specific actin is found in rhabdomyoblasts, while smooth muscle actin is found in leiomyosarcomas.
Adrenocorticotropic Hormone (ACTH or Corticotropin) is a peptidic hormone synthesized in the anterior pituitary gland. The primary application of Anti-ACTH is in the identification of pituitary tumours and the study of pituitary disease. The Anti-ACTH antibody reacts with ACTH-producing cells (corticotrophs). It may also cause paraneoplastic syndromes by secreting ACTH from other tumours, such as some small cell carcinomas of the lung.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC503
Antibody Isotype:
IgG1
GMDN Code:
56764
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Skeletal Muscle
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
5-methylcytosine (5-mC) is formed from the DNA methylation of the 5-carbon found on the cytosine ring. A 5-methylcytosine monoclonal antibody is a useful tool in identifying and discriminating between the unmodified cytosine base (C) and the methylated cytosine base (5-mC) as part of DNA methylation studies. DNA methylation plays an important role in the repression of transcription in the genome. When present in promoter regions, 5-mC is associated with stable transcriptional silencing which results in inactivation of gene function, thereby having an important role in tumorigenesis.
Calcitonin is a polypeptide hormone formed by the proteolytic cleavage of a larger prepropeptide. It is produced primarily by the parafollicular C-cells of the thyroid, and is involved in the regulation of calcium and phosphorus metabolism. It decreases the level of calcium and phosphate ions in blood by promoting the incorporation of these ions into bones, as well as inhibiting renal tubular cell reabsorption. Calcitonin expression is found in C-cell hyperplasia and medullary thyroid carcinomas. It is a useful marker in the identification of C-cell proliferative abnormalities, and for distinguishing medullary carcinoma from papillary and follicular thyroid cancer.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC521
Antibody Isotype:
IgG
GMDN Code:
56870
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Thyroid, Thyroid Medullary Carcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
The Cadherin-17 [IHC520] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissue within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist
CA-125 is normally found in epithelial cells of Fallopian tube, endometrium and endocervix, pancreas, colon, gall bladder, stomach, kidney, apocrine sweat gland, mammary gland, and mesothelial cell lining of pleura, pericardium, and the peritoneum. Anti-CA-125 reacts positively with ovarian malignancies, cervical carcinoma, seminal vesicle carcinoma, anaplastic lymphoma, and endometrial and bladder adenocarcinoma.
CA 19-9 is a secreted protein that is implicated in various cancers. It is overexpressed in salivary gland mucoepidermoid carcinomas and gastric, pancreatic, and colonic (gastrointestinal) adenocarcinomas, but is not expressed in breast, kidney, and prostate carcinomas. CA 19-9 staining is also implicated in Mirizzi’s Syndrome or other bile duct and liver diseases.
The C4d [IHC519] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissue within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
Bile Salt Export Pump (BSEP) is a member of the ATP-binding cassette (ABC) transporters, and mediates the transport of bile acid, taurocholate, and other cholate conjugates across the hepatocyte canalicular membrane into the canaliculus. BSEP is associated with progressive familial intrahepatic cholestasis type 2 (PFIC2) and benign recurrent intrahepatic cholestasis type 2 (BRIC2). PFIC2 caused by mutations in the BSEP gene increases the risk of hepatocellular carcinoma in early life.
Serine/Threonine-Protein Kinase B-Raf (BRAF) is a cytoplasmic serine-threonine kinase of the RAF family, which mediates downstream cellular responses to growth signals through the mitogen-activated protein kinase (MAPK) signaling pathway. Oncogenic mutations in the BRAF gene, 80% of which are a single V600E substitution within the kinase domain, constitutively activate the MAPK signaling pathway and result in increased cell proliferation and apoptosis resistance. The V600E mutation is observed in colorectal cancer, non-Hodgkin's lymphoma, papillary thyroid carcinoma, malignant melanoma, non-small-cell lung carcinoma, and lung adenocarcinoma. BRAF V600E is therefore an important immunohistochemical marker for tumour diagnosis and prognosis.
BOB-1 is a B-cell-specific coactivator whose expression majorly restricted to mature B-cells, and typically in germinal center B-cells. It is co-activator for Oct-1 and Oct-2 transcription factors. BOB-1 is used for B-lineage determination of CD20- plasmablastic or diffuse large B-cell lymphoma (DLBCL). BOB-1 was also presented to be useful marker when combined together with CD79a and Cyclin E for discriminating classical Hodgkins lymphoma from primary mediastinal large B-cell lymphoma.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC060
GMDN Code:
62794
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Tonsil
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
BG8 Lewis y, also known as Lewis<sup>y</sup> Blood Antigen or simply BG8, is a blood group antigen that has been identified in many studies as a potential marker for differentiation between pulmonary adenocarcinoma (PACA) and epithelioid mesothelioma (EM). It has been reported that sensitivity of non-mesothelial antigens for adenocarcinoma is organ-dependent. When attempting to differentiate epithelioid mesothelioma from adenocarcinoma, BG8 Lewis<sup>y</sup> performed at a sensitivity of 98% in the breast cancer group, and 100% in the lung cancer group.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC517
Antibody Isotype:
IgG1, kappa
GMDN Code:
63793
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Lung Adenocarcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
β-Catenin is a cytoplasmic protein with a dual role in cell-cell adhesion and gene expression. It is normally present in the submembranous regions of the cell, and nuclear accumulation of β-Catenin has been found to occur as a result of gene mutations. This accumulation is useful in identifying desmoid tumours (fibromatosis) in the abdomen and breast, and is therefore useful in differentiating other cell neoplasms in these regions.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC516
Antibody Isotype:
IgG1
GMDN Code:
56911
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Fibromatosis of Breast
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
B-Cell Lymphoma 6 (BCL6) is a zinc finger transcription factor. BCL6 expression is seen in follicular lymphomas, Burkitt's lymphoma, angioimmunoblastic T-cell lymphoma, and nodular lymphocyte-predominant Hodgkin's lymphoma. Together with BCL2, BCL6 is often used to distinguish neoplastic follicles from those in benign hyperplasia, and to aid in the classification of mantle cell lymphomas and nodular lymphocyte-predominant Hodgkin's lymphoma.
B-Cell Lymphoma 2 (BCL2) is involved in regulation of cell apoptosis by controlling mitochondrial permeability and release of cytochrome c. It also has critical roles in normal cell physiology related to neuronal activity, autophagy, calcium handling, mitochondrial dynamics, and energetics. BCL2 overexpression has been shown to promote cell survival by suppressing apoptosis, and is found to be correlated with poor disease prognosis in breast, prostate, ovarian, endometrial, and colon cancers. In follicular lymphoma, Anti-BCL2 reacts negatively with germinal centers and positively with neoplastic follicles. In lymphoid lesions, BCL2 staining is useful for distinguishing reactive and neoplastic follicular proliferations, and for identifying minimal residual disease in the bone marrow of follicular lymphoma patients. BCL2 is now a useful target of human cancer therapy.
The BCA-225 [IHC225] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissues within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC225
GMDN Code:
?
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Breast Carcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
The IgG4 [IHC114] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissues within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Applications:
IHC
Clone number:
IHC114
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Tonsil
Buffer:
Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
Indoleamine 2,3-dioxygenase 1 (IDO1) is a cytoplasmic enzyme encoded by the INDO gene on human chromosome 8p22. IDO1 modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in immune evasion and tumor outgrowth. Blocking the IDO1 pathway may be a potential target for immuno and cancer therapy. IDO1 expressions have been found in endometrial, cervical carcinomas, renal cell carcinomas, non-small cell lung carcinomas, and colorectal carcinomas.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC101
GMDN Code:
66572
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Tonsil
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Isocitrate Dehydrogenase 1 (IDH1) is a soluble, cytoclic enzyme involved in the TCA metablic cycle. The most notable mutation in this enzyme, R132H, is clinically indicated in the majority of astrocytomas and oligodendroglial tumours, with the mutation being associated with more favourable prognosis and increased survival in those patients. IDH1 R132H is also useful in the differential diagnosis between anaplastic glioma and glioblastoma.
HMB-45 is specific for an antigen present in immature melanosomes, cutaneous melanocytes, and prenatal and infantile retinal pigment epithelium cells. It is therefore effective for identifying malignant melanoma, and differentiating metastatic amelanotic melanoma from a number of conditions where the discrimination is often extremely difficult, including large cell lymphomas, sarcomas, spindle cell carcinomas, and various types of mesenchymal neoplasms. This antibody can also differentiate between junctional nevus and intradermal nevus cells, and between fetal or neonatal melanocytes and normal adult melanocytes.
CD2 is a transmembrane glycoprotein that is expressed on peripheral blood T lymphocytes, NK cells and thymocytes. Interaction between CD2 and its counter receptor LFA3 (CD58) on opposing cells optimizes immune system recognition, thereby facilitating communication between helper T lymphocytes and antigen presenting cells, as well as between cytolytic effectors and target cells. EBSCD-003 reacts with human T-cells, leukemic T-cells and T-cell lines. EBS-CD-003 also reacts with some, if not all, E-RFC-receptors on K and NK cells.
Antibody Isotype:
IgG2b-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-003
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Thurlow PJ, et al., transplantation 36: 293-298 (1983)
References 2:
Kozarsky KF, et al, Cell Immunol 150: 235-246 (1993)
References 3:
Schlossman SF et al. Leukocyte Typing?V Oxford?University Press:?342-352 (1995)
109-3C2 binds with CD20 which is a 30/33 kDa non-glycosylated transmembrane phosphoprotein with three extensive hydrophobic regions. CD20 is involved in regulation of B-cell activation. It is expressed on the surface of all B-cells beginning at the pro-B phase (CD45R+, CD117+) and progressively increasing in concentration until maturity. Plasma cells are negative. CD20 is retained on many B-cell malignancies. CD20 positive cells are also sometimes found in cases of Hodgkins disease, myeloma, and thymoma. 109-3C2 has been clustered at IVth and Vth HLDA Workshops.
Antibody Isotype:
IgG3-K
Monosan Range:
MONOSAN
Clone:
109-3C2
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Knapp W. Leukocyte Typing IV, Oxford Univ. Press, (1989)
References 2:
Schlossman SF et al. Leukocyte Typing?V Oxford?University Press:?342-352 (1995)
93-1B3 binds with CD20 which is a 30/33 kDa non-glycosylated transmembrane phosphoprotein with three extensive hydrophobic regions. CD20 is involved in regulation of Bcell activation. It is expressed on the surface of all B-cells beginning at the pro-B phase (CD45R+, CD117+) and progressively increasing in concentration until maturity. Plasma cells are negative. CD20 is retained on many B-cell malignancies. CD20 positive cells are also sometimes found in cases of Hodgkins disease, myeloma, and thymoma. 93-1B3 has been clustered at the IIIrd and Vth HLDA Workshops.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
93-1B3
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Cobbold, S. et al., in leucocyte typing III, Oxford University Press (1987)
References 2:
Schlossman SF et al. Leukocyte Typing?V Oxford?University Press:?342-352 (1995)
FR5A10 reacts with CD21, a 140 kDa cell surface molecule which acts as a receptor for EBV, for human complement factor C3d (CR2) and for IFN-alpha. It is a glycoprotein, made up of multiple (n=15) Short Consensus Repeats (S.C.R.) sequences. FR5A10 has been assigned to (S.C.R.) sequences. FR5A10 has been assigned to S.C.R. numbers 5-8. FR5A10 is highly specific to CR2 and shows no cross-reaction with CR1. CD21 is expressed strongly on mature B-cells, follicular dendritic cells and weakly on immature thymocytes and T-lymphocytes. In B-cell ontogeny, CD21 appears after the preB-stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells. CD21 expression is also gradually lost after stimulation of B-cells in vitro.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
FR5A10
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Schlossman SF et al. Leukocyte Typing?V Oxford?University Press:?342-352 (1995)
References 2:
Aubry JP et al. Leukocyte Typing V, p535-536, Oxford University Press, Oxford, (1995)
FR10B4 reacts with high affinity to CD22, which is expressed in the cytoplasma of all B-cells, appearing as early as cell-surface CD19 during B-cell development. Its present on the surface of most mature sIg+ B-cells with especially high expression on hairy cell and prolymphocytic leukemia cells. CD22 is a member of the immunoglobulin super-family and acts as an adhesion molecule: BL-CAM. On frozen sections, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, while germinal centre B-cells react relatively weakly.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
FR10B4
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Campana, D., et al.Leucocyte Typing IV, Oxford Univ. Press, (1989), pp 190-192
EBS-CD-017 reacts with CD25 (55 kDa) which associates as alpha chain with CD122 and the common gamma chain (CD132) to form the high-affinity IL-2 receptor complex. With respect to lymphomas, CD25 is present on malignant cells of Hodgkins disease, HTLV-1+ adult T-cell leukemia, cutaneous T-cell lymphoma, and hairy cell leukemia. Increased levels of soluble CD25 are observed in leukemias/lymphomas and inflammatory/ autoimmune diseases. CD25 alone appears to function as a low affinity receptor and associates with CD122 (IL-2R chain, p75) and CD132 (common chain) to form the high affinity IL-2 receptor complex. CD25 antibodies detect three epitope regions, A, B and C. EBS-CD-017 recognizes B epitope, which is located at residue 3-104 of CD25 and does not block IL-2 binding to CD25. CD25 antibodies have been used successfully as a carrier for cytotoxic drugs enabling specific delivery to IL-2RA displaying target cells.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-017
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Yamamura T. et al, Eur J Surg 168(1): 49-54 (2002)
References 2:
Lundin K. et al, Anal Biochem 299(1): 92-7 (2001)
References 3:
Raivio E. et al, APMIS 105(2): 108-14 (1997)
References 4:
Boutin B. et al, Neuropediatrics 20(4): 202-6 (1989)
B-B12 reacts with 5 invariable CD3-chains: CD3y or gp26, CD3d or gp20, CD3e or gp20, CD3f or p16 (homodimer), CD3n or p28. Molecular mass: 25-28, 20 and 16 kDa. The main reactivity is with T cells, including thymocytes, mature T cells and T cell lines.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
B-B12
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Meuer SC et al, Nature 303(5920): 808-810 (1983)
References 2:
Reinherz et al, Cell 30: 715 (1982)
References 3:
Borst et al, J. Biol. Chem. 258: 5135 (1983)
References 4:
Van den Elsen et al, Nature 312 (5993): 413-8 (1984)
FS12 reacts with CD31 (PECAM-1), a 130-140 kDa member of the immunoglobulin gene superfamily that is expressed on cells of the vasculature, including platelets, endothelial cells, myeloid cells and certain lymphocyte subsets.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
FS12
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Sandilands, G. et al. Clin. and Exp. Immunology, 162(3): 516-27 (2010)
References 2:
Kishimoto T. et al., eds. Leukocyte Typing VI, Garland Publishing, Inc, (1997)
CD33 is a 67 kDa glycoprotein of the sialic acid-binding immunoglobulin-like lectin (SIGLEC) family, displaying the immune-receptor tyrosine-based inhibitory motif (ITIM), able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies. ITIMs are also used for ubiquitinmediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. CD33 is expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. It is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-022
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Knapp W. Leukocyte Typing IV, Oxford Univ. Press, (1989)
References 2:
Favaloro EJ et al, Dis Markers 5(4): 215-25 (1987)
References 3:
Favaloro EJ et al, Br J Haematol 69(2): 163-71 (1988).
EBS-CD-025 reacts with a 45 kDa glycopeptide which is a type II membrane glycoprotein with a transmembrane sequence near the regulation of lymphocyte adhesion to endothelial cells. Its ligand is CD31. CD38 is found on early cells of B and T lineages and on activated B- and tcells. It is not found on most mature resting peripheral lymphocytes. Also positive are thymus cells and Ig secreting plasma cells. The CD34+ and CD38- population of hematopoietic stems cells defines the most pluripotent cells (e.g. blast colony forming cells). EBS-CD-025 antibody blocks the EBS-CD-026 epitope, and vice versa.
EDU-2 reacts with human CD4, a 55 kDa single chain transmembrane glycoprotein that contains four extracellular immunoglobulin-like domains and is mainly expressed on T-helper lymphocytes, but also on cortical thymus cells, microglial and dendritic cells. It binds to HLA class II molecules during the interaction of CD4+ T cells with antigen-presenting cells or target cells. CD4 also serves as the primary cellular receptor for human immunodeficiency virus (HIV). EDU-2 was assigned to CD4 at the International Leucocyte Typing Workshops II and IV.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
EDU-2
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Knapp W. Leukocyte Typing IV, Oxford Univ. Press, (1989)
References 2:
Reinberz EL et al. eds. Leykcocyte Typing II, Springer-Verlag, Berlin, (1985)
84-3C1 reacts with a 95/115 kDa protein on T-cells and thymocytes and a 115/135 kDa molecule on neutrophils and platelets. 70-90% of T-cell lymphomas and from 22-37% of Bcell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a Blineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma. In addition, expression is altered in Wiskott Aldrich syndrome. A proportion of AIDS patients have antibodies to CD43. A soluble form called galactoglycoprotein is present in serum. 85-3C1 was typed at the 3rd International Workshop on Human Leucocyte Differentiation antigens.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
84-3C1
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Cobbold, S. et al. In Leucocyte typing III, Oxford University Press, pp 789-803 (1987)
References 2:
Stross, WP. et al. J. Clin. Path. 42: 953-961 (1989)
145-23 reacts with CD44, a type 1 transmembrane glycoprotein providing cell-cell and cellmatrix adhesion while linked to the cytoskeleton. It is involved in haematopoiesis, lymphocyte homing and activation, and tumor metastasis. It binds to fibrin, hyaluronate and other matrix proteins. On tumors CD44H is highly expressed, suggesting an important role in progression for this isoform. CD44 also forms the protein backbone of the human erythrocyte Lutheran antigen system. The epitope of 145-23 is resistant to (chemo)trypsin digestion.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
145-23
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Nishina S. et al., Graefes Archiv. Clin. Exp. Opthalm. 235: 92-96 (1997)
References 2:
Horny H.P. et al. Virchows Arch. 429: 91-94 (1996)
CD45 glycoprotein have various molecular weight on various cell types: B-cells 240 kDa, thymocytes 180 kDa, T-cells multiple bands. Reduced in PAGE gels: 180 and 240 kDa. Isoforms are produced by alternative splicing of domains 4, 5 and 6. Various isoform are expressed differently on different lymphocytes. All hematopoietic cells express CD45 proteins except erythrocytes. Relevant epitopes are termed CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45R or CD45R0 (exon 4-6 spliced out).
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
Bra55
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Chorváth et al., Neoplasma 34(6), 685-692, (1987)
References 2:
Chorváth et al., Neoplasma 35(5), 495-501, (1988
References 3:
Chorváth et al. Leukocyte Typing IV, pp. 634-637, (1989)
EBS-CD-028 reacts with CD46 or Membrane Cofactor Protein (MCP; 52-74 kDa) All nucleated human cells carry CD46, which has multiple common protein isoforms of 52 kDa to 66 kDa, and 74 kDa on placental cells and some transformed cells. CD46 acts as a cofactor for complement factor I, a serine protease, which protects autologous cell against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. It may further be involved in the fusion of the spermatozoa with the oocyte during fertilization. CD46 acts as a co-stimulatory factor for T-cells, which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity. A number of viral and bacterial pathogens seem to exploit this property and directly induce an immunosuppressive phenotype in T-cells by binding to CD46.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-028
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Iwata, K., et al. J. Biol. Chem. 270: 15148-15152 (1995)
References 2:
Liszewski, M.K., et al. Adv. Immunol. 61: 201-283 (1996)
References 3:
Liszewski, M.K., et al. J. Immunol. 156: 4415-4421 (1996)
Cris-1 reacts with a 67 kDa protein, consistent with human CD5. Cris-1 was assigned at the Ist and IIIrd International Leucocyte Typing Workshops. CD5 is a pan T-cell marker that also reacts with a Bcell marker subset and a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10%) of diffuse large Bcell lymphoma. CD5 aberrant expression is useful in the diagnosis of mature T-cell neoplasms.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
Cris-1
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Proceedings of the first international workshop and conference on human leukocyte differentiation antigens. Oxford University Press, Oxford (1983).
References 2:
Leukocyte Typing III, McMichael A. J. et al. (Eds.), Oxford University Press, Oxford (1987)
References 3:
Alberola-Ila J, et al, J Immunol. 148(5): 1287-93 (1992)
References 4:
Arrizabalaga P. et al, Nephron 53: 41-49 (1989)
References 5:
Guarne A. et al, Protein Science 5: 167-169 (1996)
101-1D2 reacts with the cellular adhesion molecule CD50 (ICAM-3), a single chain polypeptide with a MW of 12 kDa. The protein is heavily glycosylated and resistant to phosphatidylinositol phospholipase C treatment so probably not PO-anchored. 101-1D2 was provisionally clustered as CDw50 at the IV and confirmed as CD50 at the V international workshop on human leucocyte differentiation antigens.
CD53 is a 33-55 kDa protein expressed on monocytes and macrophages, dendritic cells, osteoblasts and osteoclasts, and on B- and T-cells from every stage of differentiation but is absent from platelets, red blood cells. CD53 appears to be the marker with widest reactivity as well as the marker with the strictest specificity to hematopoietic cells. 161-2 Partially inhibits T-cell proliferation induced by CD3 UCHT-1 antibody. 161-2 was typed in Kobe, Japan at the VIth International Workshop on human leucocyte differentiation antigens.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
161-2
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Kishimoto T. et al., eds. Leukocyte Typing VI, p509-514, Garland Publishing, Inc, (1997)
F4-31C2 reacts with CD54 or ICAM1 (Intercellular Adhesion Molecule 1). ICAM1 belongs to the immunoglobulin superfamily, C2 subset, is a transmembrane molecule of 90 kDa with 7 potential N-glycosylation sites. It is expressed on resting monocytes and endothelial cells and in response to inflammatory cytokines such as TNF-alpha, IL1 and IFN-gamma, can be highly upregulated on many other cells, e.g. on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrin CD11a/CD18 (LFA 1) and to CD1b/CD18 (Mac 1). The interaction of CD54 with LFA 1 enhances antigen specific T-cell activation. CD54 also binds to CD43, fibrinogen, most human rhinoviruses and to Plasmodium falciparum infected erythrocytes. ICAM1 may also be related to progression and metastasis of tumors.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
F4-31C2
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Johnson, J.P., et al., in Knapp, W., et al. Leucocyte Typing IV, pp 681-683 (1989)
F4-29D9 reacts with CD55 or DAF (Decay Accelerating Factor). All leucocytes as well as human erythrocytes, fibroblasts, platelets, endothelial cells and neuroectodermal cells are positive for DAF. F4-29D9 also recognizes an antigen on spermatozoid cells. It is a glycosylphosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. CD55 also serves as receptor for CD97 and for echovirus and coxsackie B virus. DAF is deficient in both granulocytes and monocytes in patients with paroxysmal nocturnal haemoglobinuria.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
F4-29D9
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Kishimoto T. et al., eds. Leukocyte Typing VI, p509-514, Garland Publishing, Inc, (1997)
References 2:
B.E. Loveland - in Leucocyte Typing VI - Part 6 CD55 Workshop Panel Report pp519-520, (1997)
References 3:
Ruix-Delgado, GJ et al, Hematology 14: 33-7 (2009)
EBS-CD-033 reacts with an extracellular domain (close to the cell membrane) of CD56/NCAM. Only 2 isoforms of NCAM are recognized by EBS-CD-033 (180 kDa and 145 kDa). EBS-CD-033 was used successfully for immunoscintigraphy and immunotherapy of SCLC xenografts in nude mice. EBS-CD-033 recognizes NCAM in retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell lung carcinomas. NCAM is also expressed on some mesodermally derived tumors (a.o. rhabdomyosarcoma). Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-033
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Schol DJ. et al, Int. J. Cancer Suppl.2: 35-40 (1988)
References 2:
Kibbelaar RE. et al, Eur J Cancer 27(4): 431-5 (1991)
References 3:
Brezicka FT. et al, APMIS. 99(9): 797-802 (1991)
References 4:
Takaku H. et al. Am J Gastroenterol. 94(5): 1402-4 (1999)
References 5:
Kaufman O. et al, Hum. Pathol. 28(12): 1373-1378 (1997)
CD59, or protectin, is a 18-22 kDa cell surface molecule on an GPI anchor. It regulates complementmediated cell lysis and is supposed to protect normal and tumor cells from cytotoxic attack by homologous complement through binding to C8 and C9. CD59 is expressed on leucocytes, vascular epithelium, a variety of epithelial cells and placenta. B-cell express low levels. The expression of CD59 on erythrocytes is important for their survival. Genetic defects in GPI-anchor attachment, that cause a reduction or loss of CD59 and CD55 on erythrocytes produce the symptoms of the disease Paroxysmal nocturnal hemoglobinuria (PNH). 193-27 Was typed at the VIth International Workshop on human leucocyte differentiation antigens.
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
193-27
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Kishimoto T. et al., eds. Leukocyte Typing VI, p509-514, Garland Publishing, Inc, (1997)
References 2:
Shichishima T. et al. Br J Haematol, 85(2):378-386 (1993)
References 3:
Navenot JM. et al. Transfusion 38(4):337-342 (1998)
References 4:
Murray EW et al, J Biol Chem, 273(39):25279-25284 (1998)
EBS-CD-005 recognizes the CD6 molecule, a single chain transmembrane glycoprotein of 120 kDa, which is expressed on the majority of mature T-cells an mature thymocytes. A B-cell subset is weakly positive and B-CLLs may also be reactive. CD6 is a type 1 transmembrane glycoprotein that is tyrosine phosphorylated during TCR-mediated T-cell activation. CD6 shows significant homology to CD5. Antibodies to CD6 are used to deplete T-cells from bone marrow transplants to prevent graft versus host disease.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-005
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Yssel et al., Cell. Immunol. 105: 161 (1987)
References 2:
Kamoun et al., J. Immunol. 127: 987 (1981)
References 3:
Reinherz et al., Proc. Nat. Acad. Sci. 79: 6047 (1982)
EBS-CD-034 reacts with a complex of CD41 and CD61, i.e. alpha IIb integrin and the integrin beta chain. The MW of the GPIIIa is 105 kDa unreduced and 90 kDa reduced. The integrin beta 3 chain can also form a complex called the vitronectin receptor with integrin alpha V: the CD51/CD61 complex. Ligands are fibrinogen, fibronectin, von Willebrand factor, vitronectin and thrombospondin. Residues 237-248 of GPIIIa or CD61 are critical in adhesive protein binding. The CD51/CD61 complex is also found on endothelial cells, some B-cells, monocytes/macrophages and tumor cells.
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-034
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Burns et al., Cell 45, 269 280 (1986)
References 2:
McMichael AJ et al. (eds) Leukocyte Typing III, Oxford University Press, Oxford, (1987)
References 3:
Schlossman S. et al. (eds) Leukocyte Typing V, Oxford University Press (1995)
EBS-CD-036 reacts with CD63 and is mainly used in combination with EBS-CD-147 and/or anti-PEM (MUC1) to identify melanoma from carcinoma in paraffin sections. Melanomas are EBS-CD-036 and EBS-CD-147 positive, but PEM negative. EBS-CD-036 reacts in frozen sections with melanoma and breast cancers, smooth muscle and lung (weakly). In paraffin sections melanomas (primary skin, uveal and choroidal), melanoma metastases, clear cell CA, carcinoids, skin nevi, medullary CA of thyroid, prostate CA, some breast, ovary, lung, colorectal and bladder CA positive. Normal tissues that are positive include: mast cells, sweat glands, Islets of Langerhans, pituitary, pancreas, peribronchial glads, Paneth cells and prostate glands.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-036
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Vennegoor C. et al., Int. J. Cancer 35: 287-295 (1985)
References 2:
Palmer AA et al., Pathology 17: 335-339 (1985)
References 3:
Hagen EC et al., Histopathology 10: 689-700 (1986)
References 4:
Duffield, A., et al. Proc. Natl. Acad. Sci. USA 100: 15560-15565 (2003)
CB-30 reacts with CD66e or CEA with MW of 80-200 kDa. CEA is present in fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Ant-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbladder, urachus, salivary gland, ovary, and endocervix.
BF12 recognizes 40 kDa CD7, a member of the immunoglobulin gene superfamily and expressed on the majority of immature and mature T-lymphocytes, and T-cell leukemia. It is also found on natural killer cells, a small subpopulation of normal B-cells and on malignant B-cells. CD7 associates directly with phosphoinositol 3-kinase. CD7 ligation induces production of D-3 phosphoinositides and tyrosine phosphorylation.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
BF12
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Wang, MY et. al, Bone Marrow Transplant. 9(5): 319-23 (1992)
124-1D1 recognizes 40 kDa CD7, a member of the immunoglobulin gene superfamily and expressed on the majority of immature and mature T-lymphocytes, and T-cell leukemia. It is also found on natural killer cells, a small subpopulation of normal B-cells and on malignant B-cells. CD7 associates directly with phospoinositol and tyrosine phosphorylation. 124-1D1 was assigned at the IVth International Workshop.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
124-1D1
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Knapp, W. et. al. Leucocyte Typing IV, p541, 667-670, 1087, Oxford Univ. Press (1989)
The transferrin receptor is a type II membrane glycoprotein existing as a homodimer of 180- 190 kDa with interchain disculphide bonding. The ligand is the serum iron transport protein transferrin. CD71 is expressed weakly on all resting leucocytes but is upregulated on all cells upon activation, reflecting the iron dependence of proliferation. In other tissues CD71 is expressed on most dividing cells, but also strongly on brain endothelium and alveolar macrophage. CD71 expression can reflect clinical behaviour or response to therapy in a number of malignancies including leukemia, lymphoma and breast cancer.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-040
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Van de Rijn et al. Cytogenet.Cell Genet. 36: 525 (1983)
References 2:
Oudermans et al. Cancer. 58: 1252 (1986)
References 3:
Beguin Y, et al, Leukemia (12): 2019-25 (1993)
References 4:
Rittenhouse-Diakun K, et al. Photochem Photobiol. 61(5): 523-8 (1995)
CD80 is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is included by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation.
Antibody Isotype:
IgG2b-K
Monosan Range:
MONOSAN
Clone:
C80-99
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Peach, R.J., et al. J. Biol. Chem. 270: 21181-21187 (1995)
References 2:
Fargeas, C.A., et al. J. Exp. Med. 182: 667-675 (1995)
EBS-CD-42 reacts with CD86, a member of the immunoglobulin superfamily, and highly expressed on monocytes, dendritic cells and stimulated B-cells. It is probably the major CD28 ligand. Furthermore, EBS-CD-042 blocks binding of soluble CD152 to CD86.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-042
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Giguère JF et al, J Virol 78: 6222-32 (2004)
References 2:
Mauri D et al, J Immunol 155: 118-27 (1995)
References 3:
Schlossman S. et al. (eds) Leukocyte Typing V, Oxford University Press (1995)
References 4:
Kishimoto T. et al., eds. Leukocyte Typing VI, p509-514, Garland Publishing, Inc, (1997)
References 5:
Sandilands GP et al, Immunology 108: 329337 (2003)
RIV11 recognizes a protein of 32 kDa, identified as CD8a. The CD8 molecule consists of ? and ? chains, which are disulphide-linked into heterodimers or homodimers. CD8 is expressed on a T-cell subset (cytotoxic/suppressor T-cells), thymocytes and NK-cells. The majority of CD8+ T-cells express CD8 as heterodimer. Some subpopulations of CD8+ T-cells as well NK-cells may express homodimer. CD8 functions as a co-receptor in concert with TCR for binding the MHC class I/peptide complex. HIV-2 envelope glycoprotein binds CD8 ? chain but not CD8 ? chain.
EBS-CD-009 recognizes a protein of 32 kDa, identified as CD8a. The CD8 molecule consists of ? and ?chain, which are disulphide-linked into heterodimers or homodimers. CD8 is expressed on a T-cell subset (cytotoxic/suppressor T-cells), thymocytes and NK-cells. The majority of CD8+ T-cells express CD8 as heterodimer. Some subpopulation of SD8+ T-cells as well as NK-cells may express homodimer. CD8 functions as a co-receptor in concert with TCR for binding the MHC class I/peptide complex. HIV-2 envelope glycoprotein binds CD8 ?-chain but not ?-chain.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-009
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Knapp W. et. al. Leukocyte Typing IV, p342-343, (1989)
References 2:
Parnes JR, Semin Immunol 6: 221-229 (1994)
References 3:
Delon J. et al. Immunity 9(4): 467-73 (1998)
References 4:
Akimoto H, et al. Immunology 95(2): 214-218 (1998)
143-44 recognizes a protein of 32 kDa, identified as CD8a. The CD8 molecule consists of ? and ?-chains, which are disulphide-linked into heterodimers or homodimers. CD8 is expressed on a T-cell subset (cytotoxic/suppressor T-cells), thymocytes and NK-cells. The majority of CD8+ T-cells express CD8 as heterodimer. Some subpopulation of CD8+ T-cells as well as NK-cells may express homodimer. CD8 functions as a co-receptor in concert with TCR for binding the MHC class I/peptide complex. The HIV-2 envelope glycoprotein binds CD8 ?-chain but not ?-chain. 124-1D1 was assigned at the IVth International Workshop.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
143-44
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Knapp, W. et. al. Leucocyte Typing IV, p1076, Oxford Univ. Press (1989)
IBL-3/25 is directed against the ?-chain of CD8. The CD8 complex consists of a disulphide-linked ?/? heterodimer with a MW of 30 kDa or an ?/? homodimer with a MW of 32 kDa. The CD8 molecule binds to HLA class I molecules during interaction of CD8+ T-cells with antigenpresenting cells or with target cells. CD8+ T-cells include most of the cytotoxic T-cells.
Antibody Isotype:
IgG-K
Monosan Range:
MONOSAN
Clone:
IBL-3/25
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Knapp W. et. al. Leukocyte Typing IV, p342-343, (1989)
References 2:
Parnes JR, Semin Immunol 6: 221-229 (1994)
References 3:
Delon J. et al. Immunity 9(4): 467-73 (1998)
References 4:
Akimoto H, et al. Immunology 95(2): 214-218 (1998)
EBS-CD-007 recognized a protein of 32 kDa, identified as CD8b. The CD8 molecule consists of ? and ? chains, which are disulphide-linked into heterodimers or homodimers. CD8 is expressed on a T-cell subset (cytotoxic-suppressor T-cells), thymocytes and NK cells. The majority of CD8+ T-cells express CD8 as heterodimer. Some subpopulation of CD8+ T-cells as well as NKcells may express homodimer. CD8 functions as a co-receptor is concert with TCR for binding the MHC class I/peptide complex. HIV-2 envelope glycoprotein binds CD8 ? chain but not ? chain.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-007
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Knapp W. et. al. Leukocyte Typing IV, p342-343, Oxford University Press, 1989
References 2:
Parnes JR, Semin Immunol 6: 221-229 (1994)
References 3:
Hadi Hossein Nataj Arab et al., Gastroenterol Hepatol Bed Bench. 8(2): 132139 (2015)
EBS-CD-045 reacts with human CD100, a 150 kDa homodimer cell-surface antigen that is expressed on resting and PHA-stimulated T-cells. It is absent from bone marrow, erythrocytes, eosinophils and endothelial cells. The protein is weakly expressed on NK-cells, EBV transformed B-cells, monocytes and tumor T-cell lines. It plays a role in homotypic cell adhesion and in T-cell activation.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-045
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Hall K, et al. P. Natl. Acad. Sci. USA 93: 11780 (1996)
References 2:
Mizrahi S, et al. PLoS One. 2(9): e818 (2007).
References 3:
Yoshino N, et al.. Exp. Anim. (Tokyo) 49: 97 (2000)
CD109 is a GPI-anchored member of the alpha-2-macroglobulin (A2M) and complement family of proteins. It is expressed on activated T-cells, platelets, hematopoietic stem cells, megakaryocyte precursors, vascular endothelial cells, basal and myoepithelial cells of secretory glands, and squamous cell carcinomas. A 170-180 kDa precursor is autocatalytically reduced to 150 kDa and 120 kDa forms. On keratinocytes CD109 binds TGF-beta and associates with TGFbeta RI and TGF-beta RII, resulting in inhibition of TGF-beta signalling. Polymorphisms of CD109 include the platelet-specific Gov antigen and the blood group ABH antigens. Alloantibodies directed against these antigens result in unsuccessful platelet transfusions, neonatal alloimmune thrombocytopenia, and post-transfusion purpura.
EBS-CD-060 reacts with a peptide epitope on the extracellular domain of human Glycophorin A, a 39 kDa sialoglycoprotein, present on red cells and erythroid precursor cells. Glycophorin A is the carrier of blood group M and N specificities, while Glycophorin B carries S and U specificities. Providing a mucin like coat, Glycophorin may play a role in preventing red cell aggregation in the circulation. Glycophorin also acts as receptors for Sendai and Parvovirus
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-060
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Cartron JP et al, Transfus Med Rev 6(2): 63-92 (1992)
Ep-CAM (also called ESA, EGP40, 17-1A antigen, KSA, GA7333-2) is a 40 kDa epithelial protein expressed on baso-lateral cell surfaces in very many epithelial tissues (but absent from mesothelial tissues). The 324AA have 3 potential glycosylation sites and is a transmembrane glycoprotein. The extracellular domain has a cysteine-rich repeat and a small domain with homology to nidogen. It is a homophilic cell-cell adhesion molecule (Ep-CAM). EBS-CD-061 reacts with most epithelial cells and carcinomas.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-061
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Edwards DP et. al. Cancer Res 46:1306-17 (1986)
References 2:
Litvinov et al. J. Cell. Biol. 125: 437-446 (1994)
203-6 Reacts with human CD27, a disulphide-linked 120 kDa dimer. CD27 is a lymphocyte-specific member of the TNF-R/NGF-R superfamily, and is expressed on a subset of human thymocytes and on the majority of mature T-lymphocytes. CD27 is highly expressed on activated T and B-cells. 203- 6 was clustered at the VIth WLDA.
Antibody Isotype:
IgG3-K
Monosan Range:
MONOSAN
Clone:
203-6
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Kishimoto T. et al., eds. Leukocyte Typing VI, p509-514, Garland Publishing, Inc, (1997)
CD36 is a 80-90 kDa protein, expressed on platelets, monocytes and macrophages, microvascular endothelial cells, erythrocyte precursors, mammary epithelial cells, and some macrophage derived dendritic cells. CD36 acts as a receptor for thrombospondin (TSP), collagen types I, IV and V, P.falciparum malaria-infected erythrocytes, and sickle erythrocytes. CD36 plays a role in platelet aggregation, macrophage foam cell development, inflammation, and the tissue ischemia observed in sickle cell disease and cerebral malaria.
EBS-T-232 reacts with SITTTE in the VNTR domain of human MUC3. The mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats (VNTRs) encoded by 60 base pairs (MUC1), 69 base pairs (MC2) and 51 base pairs (MUC3). Cancer cells of colon, breast and stomach, normal cells of salivary gland, breast, lung, and gastrointestinal tract are positive for MUC3.
Antibody Isotype:
IgG2b-K
Monosan Range:
MONOSAN
Clone:
EBS-T-232
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Apostolopoulos, V. et al. J. Gastroenterol. Hepatol. 1995; 10 (5): 555-561
EBS-CD-044 reacts with CD99 or MIC2. Human thymocytes, PBLs and some T-ALL isolates and cell lines are positive. It is also present on pancreas and on Ewing sarcoma, which forms the practical application of the antibody. It is involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes.
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-044
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Rajasekariah et al. in: Leukocyte Typing III A. McMichael (ed), Oxford University Press, Oxford (1987)
VU-3C6 reacts with the protein core of MUC1, an apical cell side epithelial marker which is upregulated or switched on in the majority of carcinomas. The dominant epitope of VU-3- C6 is GVTSAPDTRPAP, located in the VNTR domain of MUC1. Binding of VU-3C6 is enhanced after glycosylation of the DTR motif.
VU-3D1 reacts with the protein core of MUC1, an apical cell side epithelial marker which is upregulated or switched on in the majority of carcinomas. The dominant epitope of VU3D1 is SAPDTRPA, located in the VNTR domain of MUC1.
VU-4H5 reacts with the protein core of MUC1, an apical cell side epithelial marker which is upregulated or switched on in the majority of carcinomas. The dominant epitope of VU-4H5 is PDTR, located in the VNTR domain of MUC1. In tissue sections, VU-4H5 also displays prominent staining of the cytoplasm.
VU-11D1 reacts with the protein core of MUC1, an apical cell side epithelial marker which is upregulated or switched on in the majority of carcinomas. The dominant epitope of VU11D1 is TSAPDTRP, located in the VNTR domain of MUC1. Binding of VU-11D1 is enhanced after glycosylation of the DTR motif.
VU-11E2 reacts with the protein core of MUC1, an apical cell side epithelial marker which is upregulated or switched on in the majority of carcinomas. The dominant epitope of VU11E2 is TSAPDTRP, located in the VNTR domain of MUC1. Binding of VU-11E2 is enhanced after glycosylation of the DTR motif
VU-12E1 reacts with the protein core of MUC1, an apical cell side epithelial marker which is upregulated or switched on in the majority of carcinomas. The dominant epitope of VU-12- E1 is PDTRPAP, located in the VNTR domain of MUC1. Binding of VU-12E1 is enhanced after glycosylation of the DTR motif
VU-13F11 reacts with the protein core of MUC1, an apical cell side epithelial marker which is upregulated or switched on in the majority of carcinomas. The dominant epitope of VU-13F11 has not yet been determined but is located in the VNTR domain of MUC1 (confirmed by ELISA).
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
VU-13F11
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Schol D. et al, Tumor Biol. 19(Suppl 1): 35-45 (1998)
VU-2G7 reacts with the protein core of MUC1, an apical cell side epithelial marker which is upregulated or switched on in the majority of carcinomas. The dominant epitope of VU2G7 includes the PDTR motif, located in the VNTR domain of MUC1. Binding of VU-2G7 is significantly enhanced when the threonine of the PDTR motif bears a GalNAc
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
2G7
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Ryuko, K. et al. Tumor Biol. 21(4): 197-210 (2000)
References 2:
Karsten, U. et al. Cancer. Res. 58(12): 2541-2549 (1998)
EBS-C-002 reacts with NuMA or Nuclear Mitotic Apparatus protein, which at the onset of mitosis redistributes from the nucleus to two centrosomal structures at the poles of the mitotic spindle, where it plays a vital role in establishing and maintaining its bipolar structure. After anaphase the protein redistributes from the spindle polar region into the reforming nucleus and concentrates initially at the site where nuclear lamins and perichomatin have been reported to assemble. In contrast to mitotic cells, post-mitotic neurons display NuMA both in the nucleus and in the cytoplasm. Due to release from dead cells, NuMA is also used as oncological marker in serum and urine. In addition, chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 has been detected in patients with acute promyelocytic leukemia.
1-13M1 recognizes the peptide core of gastric mucin M1/MUC5AC), and more specifically with the a epitope, which is the most abundant amongst the a, b, c, d, e, f, and h protein core epitopes defined by Bara for M1. MUC5AC is present in primary ovarian mucinous cancer and gastric cancer, but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification pancreatic carcinoma and precancerous changes vs. normal pancreas.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
1-13M1
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bara, J. et al., Cancer Res.46: 3983-3989 (1986)
References 2:
Bara, J. et al., Biochem. J. 254: 185-193 (1988)
References 3:
Bara, J. et al., Int. J. Cancer 47: 304-310 (1991)
References 4:
Bara, J. et al., J. Immunol. Methods 149: 105-113 (1992)
References 5:
Guyonnet Duperat V. et al., Biochem. J. 305: 211 219 (1995)
2-11M1 recognizes the peptide core of gastric mucin M1/MUC5AC, and more specifically with the b epitope amongst the a, b, c, d, e, f, g, and h protein core epitopes defined by Bara for M1. 2-11M1 and 9-13M1 react exclusively with epitopes located in the N-terminal cysteine-rich part of the peptide core MUC5AC. MUC5AC is present in primary ovarian mucinous cancer and gastric cancer, but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
2-11M1
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bara, J. et al., Cancer Res.46: 3983-3989 (1986)
References 2:
Bara, J. et al., Biochem. J. 254: 185-193 (1988)
References 3:
Bara, J. et al., Int. J. Cancer 47: 304-310 (1991)
References 4:
Bara, J. et al., J. Immunol. Methods 149: 105-113 (1992)
References 5:
Guyonnet Duperat V. et al., Biochem. J. 305: 211 219 (1995)
2-12M1 recognizes the peptide core of gastric mucin M1/MUC5AC, and more specifically with the c epitope amongst the a, b, c, d, e, f, g, and h protein core epitopes defined by Bara for M1. 2-12M1 and 45M1 both specifically react with epitopes located in the Cterminal cysteine rich part of the peptide core of gastric mucin (MUC5AC). MUC5AC is present in primary ovarian mucinous cancer and gastric cancer, but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. AntiMUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
2-12M1
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bara, J. et al., Cancer Res.46: 3983-3989 (1986)
References 2:
Bara, J. et al., Biochem. J. 254: 185-193 (1988)
References 3:
Bara, J. et al., Int. J. Cancer 47: 304-310 (1991)
References 4:
Bara, J. et al., J. Immunol. Methods 149: 105-113 (1992)
References 5:
Guyonnet Duperat V. et al., Biochem. J. 305: 211 219 (1995)
45M1 recognizes the peptide core of gastric mucin M1 (now: MUC5AC), and more specifically with the h epitope amongst the a, b, c, d, e, f, g and h protein core epitopes defined by Bara for M1. 45M1 and 2-12M1 both specifically react with epitopes located in the C-terminal cysteine rich part of the peptide core of MUC5AC. MUC5AC is present in primary ovarian mucinous cancer and gastric cancer, but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
45M1
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bara, J. et al., Int. J. Cancer 47: 304-310 (1991)
References 2:
Bara, J. et al., J. Immunol. Methods 149: 105-113 (1992)
58M1 recognizes the peptide core of gastric mucin M1 (now: MUC5AC), and more specifically with the e epitope amongst the a, b, c, d, e, f, g and h protein core epitopes defined by Bara for M1. MUC5AC is present in primary ovarian mucinous cancer and gastric cancer, but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
58M1
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bara, J. et al., Cancer Res.46: 3983-3989 (1986)
References 2:
Bara, J. et al., Biochem. J. 254: 185-193 (1988)
References 3:
Bara, J. et al., Int. J. Cancer 47: 304-310 (1991)
References 4:
Bara, J. et al., J. Immunol. Methods 149: 105-113 (1992)
References 5:
Guyonnet Duperat V. et al., Biochem. J. 305: 211 219 (1995)
9-13M1 recognizes the peptide core of gastric mucin M1/MUC5AC), and more specifically with the d epitope amongst the a, b, c, d, e, f, g and h protein core epitopes defined by Bara for M1. 9-13M1 and 2-11M1 react exclusively with epitopes located in the the Nterminal cysteine-rich part of the peptide core MUC5AC. MUC5AC is present in primary ovarian mucinous cancer and gastric cancer, but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification pancreatic carcinoma and pre-cancerous changes vs. normal pancreas
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
9-13M1
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bara, J. et al., Cancer Res.46: 3983-3989 (1986)
References 2:
Bara, J. et al., Biochem. J. 254: 185-193 (1988)
References 3:
Bara, J. et al., Int. J. Cancer 47: 304-310 (1991)
References 4:
Bara, J. et al., J. Immunol. Methods 149: 105-113 (1992)
References 5:
Guyonnet Duperat V. et al., Biochem. J. 305: 211 219 (1995)
47-8D3 reacts with macrophages and detects the well-known leukocyte L1, cystic fibrosis antigen. Detecting a single protein band of 14 kDa in Western blots of lysates of human monocytes and granulocytes, the antigen was identified as the calcium-binding protein MRP14, which is a member of the S100 family involved a.o. in regulating the cell cycle. MRP14 is also implicated in the abnormal differentiation of myeloid cells in the stroma of cancer. It is further found on squamous mucosal epithelia. When associated with MRP8 it forms the heterodimer calprotectin.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
47-8D3
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Flavell DJ. et al., J. Histochem. Cytochem. 35: 1217-1226 (1987)
References 2:
Facchetti F. et al., Am. J. Clin. Pathol. 92: 42-50 (1989)
References 3:
Bardadin KA. et al., J. Pathol. 164: 253-259 (1991)
References 4:
Goebeler M. et al., J. Leukocyte Biol. 55: 259-261 (1994)
1011 Is specific for the major vault protein, a 104-kDa highly conserved protein interacting with estrogen receptor. It is one of a series of four mAbs which recognize different epitopes of the protein. Major vault proteins have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for >70% of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
1011
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Abbondanza, C. et al, J. Cell Biol. 141, 1301-1310 (1998)
1014 Is specific for the major vault protein, a 104-kDa highly conserved protein interacting with estrogen receptor. It is one of a series of four mAbs which recognize different epitopes of the protein. Major vault proteins have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for >70% of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
1014
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Abbondanza, C. et al, J. Cell Biol. 141, 1301-1310 (1998)
1027 Is specific for the major vault protein, a 104-kDa highly conserved protein interacting with estrogen receptor. It is one of a series of four mAbs which recognize different epitopes of the protein. Major vault proteins have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for >70% of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
1027
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Abbondanza, C. et al, J. Cell Biol. 141, 1301-1310 (1998)
1032 Is specific for the major vault protein, a 104-kDa highly conserved protein interacting with estrogen receptor. It is one of a series of four mAbs which recognize different epitopes of the protein. Major vault proteins have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for >70% of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
1032
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Abbondanza, C. et al, J. Cell Biol. 141, 1301-1310 (1998)
A-492 reacts with adiponectin, an adipocytokin; adipocytokines are hormones produced in adipose tissue. Adiponectin is abundantly present in plasma and has an insulin like effect on glucose levels in the blood. Plasma adiponectin levels are found in insulin resistant patients who are obese, have diabetes mellitus type 2 or HIV-lipodystrophy. In women adiponectin levels tend to be higher than in men, which may be due to androgens suppressing adiponectin levels. Furthermore adiponectin and leptin are both indicated in regulating body weight through direct action on the hypothalamus, influencing appetite. Obese people have low adiponectin levels while levels in anorexia patients are high. Adiponectin acts as ligand for various receptors, two of which have been identified, one probably involved in carbohydrate assimilation, the other in tuning the rate of metabolism.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
A-492
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Dos Santos, E. et al, Oncol. Rep. 20: 971-977 (2008)
A-493 react with adiponectin, an adipocytokine. Adipocytokines are hormones produced in adipose tissue. Adiponectin is abundantly present in plasma and has insulin like effect on glucose levels in the blood. Plasma adiponectin levels are low in insulin resistant patients who are obese, have diabetes mellitus type 2 or HIV-lipodystrophy. In women adiponectin levels tend to be higher than men, which may be due to androgens suppressing adiponectin levels. Furthermore adiponectin and leptin are both indicated in regulating body weight through direct action on the hypothalamus, influencing appetite. Obese people have low adiponectin levels while levels in anorexia patients are high. Adiponectin acts as ligand for various receptors, two of which have been identified, one probably involved in carbohydrate assimilation, the other in tuning the rate of metabolism.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
A-493
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Dos Santos, E. et al, Oncol. Rep. 20: 971-977 (2008)
3-3A reacts with determinants of chain A type 3 and 4 and chain H type 3 and 4, but not with type 1 and 2 chain structures. It is not reactive with immunodominant A trisaccharide. Increased expression of this antigen has been observed on some tumor tissues such as gastric carcinomas, urothelial carcinomas, and colon carcinomas. 3-3A does not react with normal tissue sections of donors with blood group B and 0 but it reacts specifically with malignant tissues in these individuals.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
3-3A
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bara, J. et al. Biochem. J. 254: 185-193 (1988)
References 2:
Yasumsds, I. et al. Glycoconjugate J. 3: 187-202 (2000)
References 3:
Rouger et al. Blood transfusion and immunohematol. 30(5): 252-720 (1987)
HE-10 preferably reacts with determinants of chain A type 3 and 4 and chain H type 3 and 4, but not with type 1 and 2 chain structures. It is not reactive with immunodominant A trisaccharide. Increased expression of this antigen has been observed on some tumor tissues such as gastric carcinomas, urothelial carcinomas, and colon carcinomas. HE-10 does not react with normal tissue sections of donors with blood group B and 0 but it reacts specifically with malignant tissues in these individuals. HE-10 is applicable for red cell agglutination, tissue staining and immunofluorescence tests.
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
HE-10
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
N?mec M. et al. Vox Sang 52:125-8 (1987)
References 2:
Vanák J. et al. Neoplasma 36(4): 479-487 (1989)
References 3:
Tichý, M. et al. Neoplasma 37(4): 451-459 (1990)
References 4:
Tichý, M. et al. Acta Univ Palacki Olomuc Fac Med. 126: 57-69 (1990)
HE-193 recognizes human blood group A (monofucosyl and difucosyl A antigens with chain types 1, 2, 3, 4, 5, 6,) and Forssmann antigen, which is normally not found in humans, but can appear on malignancies. De novo or increased expression of these antigens have been observed on some tumor tissues such as gastric carcinomas, urothelial carcinomas, and colon carcinomas. HE-193 does not react with normal tissue sections of donors with blood group B and 0 but it reacts specifically with malignant tissues in these individuals. HE-193 is applicable for red cell agglutination, tissue staining and immunofluorescence tests
HEB-29 shows specific staining of erythrocytes and vascular epithelium of blood group B and AB controls and no staining in group A or O controls. However, tumors in A or O individuals may carry B antigen and stain with HEB-29. HEB-29 is applicable for red cell agglutination, tissue staining and immunofluorescence tests.
TV-1 recognizes fibronectin in frozen and paraffin sections of human, pig, mouse and rat tissues. Specifically, it stains this extracellular adhesive glycoprotein in connective tissues and blood vessels. TV-1 does not recognize the soluble dimeric form of fibronectin (plasma fibronectin) but strongly stains matrix fibronectin in tissues. Cellular fibronectin is widely distributed in the stroma of many malignant tumors
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
TV-1 (2755-8, EP-5)
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Epstein, AL. et al. Cancer Res. 55(12): 2673-80 (1995)
568 Reacts with the 8th type III repeat in the cell-binding domain of human fibronectin (220 kDa monomer; 440 kDa, dimer). Fibronectins are present in basement membranes, intestinal connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
568
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Christensen, L. et al, APMIS 98(7): 615-623 (1990)
References 2:
Christensen, L. et al, APMIS suppl. 26: 1-39 (1992)
References 3:
Yong, JL. et al, Int J Clin Exp Pathol 3(2): 210-216 (2010).
616 Reacts with the N-terminal fibrin and heparin-binding domain. Specificity was established by Western blotting with purified 29 kDa domain from two different sources. Fibronectins, 220 kDa monomer; 440 kDa dimer, are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
616
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Grant, M B, et al. Diabetes, 47: 1311-7 (1998)
References 2:
Homandberg, G.A. et al. Osteoarthritis and Cartilage 6: 231244 (1998)
References 3:
Klueh, U, et al. Biomaterials 24(22) : 3877-84 (2003)
References 4:
Ljubimov, A.V. et al. Exp. Cell Res. 165: 53040 (1986)
References 5:
Mirmalek-Sani SH. et al. Biomaterials 165: 548895 (2013)
618 Recognizes an epitope contained within the gelatin binding domain of human fibronectin Fibronectins, 220 kDa monomer; 440 kDa dimer, are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
618
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Ljubimov, A.V. et al. Exp. Cell Res. 165,53040 (1986)
Until recently, immunological markers for myeloid cells have been lacking, especially those which identify different levels of cellular differentiation. The BM series provides a new panel of monoclonal antibodies which stain early precursor and mature forms of human myeloid cells. This panel of monoclonal antibodies reacts with antigenic determinants present in normal myeloid cells and leukemias of similar derivation. BM-2 recognizes a cytoplasmic antigen expressed in mature human granulocytes (polys) residing in lymphoid and non-lymphoid tissues. It does not react with any other cell type in human tissues.
HE-182 reacts with human blood group H type 5 and type 6 as confirmed with Chembiomed synthetic oligosaccharide-BSA conjugate in ELISA. It is expressed on endothelial cells, epithelial cells and granulocytes. Increased expression of this antigen has been observed on some tumor tissues such as gastric carcinomas, urothelial carcinomas, and colon carcinomas
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
HE-182
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Rouher Ph et al. Blood transfusion and immuohaematology, Arnette France 30 (5): 353-720 (1987)
EBS-O-148 can be used in ELISA, frozen sections, FACS and Western blot, however no method is known to date to retrieve the antigen in paraffin sections. Anti-hemoglobin antibodies can be used in diagnosis of anemias and as tumor marker in stool.
The alpha interferons are involved in virus resistance in target cells for these viruses. They are known to block cell proliferation and to regulate MHC class I antigen expression. The IFN? family has over 20 genes and pseudogenes in two families (I and II), one with a mature length of 166aa and one of 172aa. Cells producing IFN? are lymphocytes, monocytes, macrophages and cell lines such as Namalwa and KGI. Bioassays for IFN? include cytopathic effect blocking, by viruses such as VSV, SFV and BMCV, on their target cells. A number of receptors for IFN? are now known and seem to be expressed on most cell types. 2-48 Is specific for human interferon ? 1 and does not cross react with human interferon ? 2.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
Feb-48
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Kontsek, P. et al. Mol Immunol. 29: 863-870 (1992)
The alpha interferons are involved in virus resistance in target cells for these viruses. They are known to block cell proliferation and to regulate MHC class I antigen expression. The IFN? family has over 20 genes and pseudogenes in two families (I and II), one with a mature length of 166aa and one of 172aa. Cells producing IFN? are lymphocytes, monocytes, macrophages and cell lines such as Namalwa and KGI. Bioassays for IFN? include cytopathic effect blocking, by viruses such as VSV, SFV and BMCV, on their target cells. A number of receptors for IFN? are now known and seem to be expressed on most cell types. 2-52 Is specific for human IFN?1 and does not cross react with human IFN?2.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
Feb-52
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Kontsek, P. et al., Mol Immunol. 29: 863-870 (1992)
Laminins are large hetero-trimeric, non-collagenous glycoproteins found in basement membranes and composed of ?, ?, and ? chains. A5 reacts specifically with ? chain 1. Alterations of basement membrane integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
A5
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Ljubimov JY et. al. Cancer Res. 61(14): 5601-5610 (2001)
References 2:
Ljubimov AV et. al. Int J Cancer 50: 562-566 (1992)
FR4A5 reacts with CD15 (220 kDa). CD15 contains the pentasaccharide lacto-n-fucopentaose III and plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance. It is also expressed on a wide variety of other tumor cells including myeloid leukemia, breast, colorectal, and lung cancer cells. Cross reactivity has been observed with Glc?1-6glc?1-4Glc, Tn, blood group H1, and maltose.
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
FR4A5
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Manimala J.C. et al. Glycobiology. 17(8): 17C-23C (2007)
References 2:
Gildersleeve J. et al. Glycobiology. 18(0): 746 (2008)
D11 reacts specifically with human monocytes and macrophages, in all sorts of tissues. The 125/135 kDa antigen is present on the cell membrane as well as within cytoplasmic structures including lysosomes, and is different from CD68. Among tumors, histiocytomas and histiocytic lymphomas are positive. In ALL, positive reaction with D11 indicates B-lineage derivation. AML are negative.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
D11
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Rudinskaya T.D. et al. Immunol Lett. 33(1): 1-7 (1992)
References 2:
Frenkel M.A. et al. Gematol Transfuziol. 40(4): 13-16 (1995)
References 3:
Tupitsyn N.N et al., Int J Cancer. 68(2): 160-163 (1996)
References 4:
Petrovichev N.N et al., Acta Cytol. 41(2): 357-363 (1997)
IPO-74 reacts with human leukemia cell line HL-60 and shows a band at MW 127 kDa (reduced) both in immunoprecipitation and Western blotting. In peripheral blood only subsets of B cells, T cells, neutrophilic and eosinophilic granulocytes are positive. All monocytes are positive. Cell lines U937 and K562 are positive, while Daudy, Raji, Jurkat and Molt-4 are negative, as are IL2-dependent T cell lines/clones.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
IPO-74
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Fleming MG, et al, J Cutan Pathol. 17(2): 77-81 (1990)
PLAP is a tissue specific, throphoblast-derived, 58 kDa, glycosyl-phosphatidylinositol (GPI)- anchored, dimeric, Zn2+ metallated glycoprotein, only found in humans, orangutans and chimpanzees, that catalyzes the hydrolysis of phosphomonoesters into an inorganic phosphate and an alcohol. It is present in the placenta and serum of pregnant women and in high frequency in gynecological and testicular cancers and in lower frequency in other tumors. The three tissue-specific APs in humans, PLAP, germ cell AP (GCAP) and intestinal AP, are 90-98% homologous. Non tissue specific AP is found in kidney, liver and bone. F5C2 binds equally well to all common allelic variants (S,F, FS and I) of PLAP and to some variants of AP from normal human testis, while antibody H7E8 reacts with all variants of AP in normal human testis.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
F5C2
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Millan J.L. et al, Eur. J. Biochem. 136: 1-7 (1983)
PLAP is a tissue specific, throphoblast-derived, 58 kDa, glycosyl-phosphatidylinositol (GPI)- anchored, dimeric, Zn2+ metallated glycoprotein, only found in humans, orangutans and chimpanzees, that catalyzes the hydrolysis of phosphomonoesters into an inorganic phosphate and an alcohol. It is present in the placenta and serum of pregnant women and in high frequency in gynecological and testicular cancers and in lower frequency in other tumors. The three tissue-specific APs in humans, PLAP, germ cell AP (GCAP) and intestinal AP, are 90-98% homologous. Non tissue specific AP is found in kidney, liver and bone. H7E8 binds equally well to all common allelic variants (S,F, FS and I) of PLAP as to AP from normal human testis, while antibody F5C2 reacts with some samples of normal human testis only.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
H7E8
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Millan J.L. et al, Eur. J. Biochem. 136: 1-7 (1983)
EBS-O-166 specifically reacts with tenascin-C, an extracellular matrix glycoprotein of 210 kD. It recognizes those forms of tenascin that are produced by both normal and hyperproliferative (also neoplastic) tissues. Tenascin/hexabrachion/cytotactin is an extracellular matrix glycoprotein, widely expressed during embryogenesis. In adults, it is restricted to certain epithelial-stromal interfaces and increases markedly in hyperproliferative diseases and in stroma of many neoplasms, including gliomas, breast, squamous and lung carcinomas.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-O-166
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Verstraeten, AA et al., Br. J. Derm. 127(6), 571-574 (1992)
1E8-G6 reacts with TGF-alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. 1E8-G6 is completely blocked by the peptide used for immunization. TGFalpha (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. In sections both cytoplasma and cell membranes are stained
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
1E8-G6
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bebók, Zs, et al. Breast Cancer Res.Treatm. 29: 229-235 (1994)
TU-02 reacts with the N-terminal domain of alpha-tubulin. Tubulin isotypes have been identified with tissue specific expression. Immunocytochemical studies using several mAbs revealed remarkable heterogeneity of tubulin within various nervous tissues. TU-02 reacts with tubulin in fixed tissues only, not in unfixed or live tissues or cells. Interphase microtubules are also stained by TU-02 in fixed tissues.
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
TU-02
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Dráber, P. et. al. Eur.J.Cell.Biol. 41: 82-88 (1986)
References 2:
Dráber, P. et. al. Histochemistry 87: 151-155 (1987)
References 3:
Dráber, P. et. al. J. Cell Science 92: 519-528 (1989)
References 4:
Smertenko et al. Eur. J. Cell. Biol. 72: 104-112 (1997)
UACA (Uveal Autoantigen with Coiled-coil domains and Ankyrin repeats) is a 1,416 amino acid nuclear membrane protein. It was originally identified as an autoantigen in patients with panuveitis, a characteristic of Vogt-Koyanagi-Harada disease, and in patients with Graves' disease. UACA was also later identified as Nucling, a mRNA differentially expressed in F9 embryonal carcinoma cells, and that is up-regulated during cardiac muscle differentiation. UACA appears to function as a pro-apoptotic protein that recruits the apaf-1- pro-caspase-9 complex for the induction of apoptosis to mediate the cell-death pathway.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
AE-5
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Yamada, K., et al. Biochem. Biophys. Res. Commun. 280: 1169-1176 (2001)
VEGF-21 reacts with Vascular Endothelial Growth Factor, also known as Vascular Permeability Factor (VEGF/ VPF) and is the key mediator of angiogenesis. The MWs are 19- 22kDa (reducing) and 38kDa-44kDa (non-reducing). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis. In addition to endothelial cells, VEGF and VEGF receptors are expressed on numerous non-endothelial cells including tumor cells.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
VEGF-21
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Tischer E. et al. J. Biol. Chem 266: 11947-11954 (1991)
EBS-huA recognizes the third constant domain (CH3) of the alpha chain of human IgA and reacts with both IgA1 and IgA2 isotypes and not with other types of immunoglobulins. IgA type antibodies are secreted by B-cells associated with mucosal epithelia and therefore indicate malignancy if found in lymphoid infiltrates at other locations. Detection of IgA antibodies to EBV in serum may indicate nasopharyngeal carcinoma. Rising IgA anti EBV levels are associated with progression of disease.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-huA
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Biewenga J. et al, Mol. Immunol. 23: 761-767 (1986)
References 2:
Biewenga J. et al, Adv. Exp. Med. Biol. 216B: 1239-1249 (1987)
References 3:
Mestecky J. et al, J. Immunol. Meth. 193: 103-148 (1996)
References 4:
Oortwijn B.D. et al, Mol Immunol. 44(5):966-73 (2007)
SC-05 reacts with a reduction resistant epitope on 80 kDa human secretory component (both free and bound to SIgA). Secretory component is differentially expressed in epithelium, thus SC-05 can identify subpopulations of epithelial cells and epithelial differentiation. Secretory component negative cell lines are not stained with SC-05.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
SC-05
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Kvale, D. et al, Int J Cancer 42(4): 638-641 (1988)
References 2:
Bartek, J. et al, Histochem 91(3): 235-244 (1989)
References 3:
Bartek, J. et al, Histochem J 22(10): 537-534 (1990)
EBS-huKappa reacts with kappa light chain In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of kappa to lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-huKappa
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Takahashi, H. et al. Pathol Res Prac 189, 300-311 (1993)
References 2:
Momose, H., et al. Hum. Pathol 23: 1115-1119 (1992)
EBS-huLambda reacts with human immunoglobulin lambda light chain (22,5 kDa). EBShuLambda does not react with T cells, monocytes, granulocytes or erythrocytes.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-huLambda
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Baryshnikov A. et al. Gemat.Transf. (Russian) N8, 4-7 (1990)
References 2:
Martinova T.et al., In: Problems in med biotech. and immunol. Infect.diseases. 11, 182-186, (1996)
EBS-IF-001 Does not react with keratin polypeptides in immunoblotting. The specificity for keratin was established on the basis of antibody reactivity with intracytoplasmic intermediate filaments in epithelial, vimentin negative, human and feline cells. Distribution of the epitope strongly suggests that EBS-IF-001 reacts with keratins 5 and 14, most probably with a heterotypic complex formed by these keratins. EBS-IF-001 reacts positively with basal cells of all stratified epithelia, with myoepithelial cells and with most squamous cell carcinomas. It is useful in immunohistochemistry for typing of basal cells.
C-46 reacts with keratins 7 (54kDa) and 17 (46kDa). On formalin-fixed paraffin embedded sections C46 reacts with keratin 7 only. Strongly positive on most simple epithelia except for stomach, small intestine and colon mucosa, hepatocytes, pancreatic acini, renal tubules, also positive on some non-cornifying epithelia.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
C-46
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bártek et al. J. of Pathol. 164: 215-24 (1991)
References 2:
Vojt?ek et al. Neoplasma 37(3): 333-342 (1990)
References 3:
Taylor Papadimitriou J. et. al. J. Cell Sci. 94: 403-13 (1989)
References 4:
Kova?ík et al. Int. J. Cancer Suppl. 3: 50-55, (1988)
References 5:
Kova?ík et al. J. Tumor Marker Oncol. 5: 219, (1990)
C-51 reacts with keratin 8 (52.5 kDa) + 18 (45 kDa) polypeptides and recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
C-51
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bártek et al. J. Pathol. 164: 215-24 (1991)
References 2:
Bártková et al. Neoplasma 38(4): 439-46 (1991)
References 3:
Wendl, J. et al. Anat. Histol Embryol. 164: 215-24 (1991)
References 4:
Thangappan, R et al. Cell. Polif. 42: 770-779 (2009)
EBS-IF-003 reacts with 53kDa (CK13) and 56.6kDa (CK10) cytokeratin proteins as indicated by immunoblotting. On formalin-fixed, paraffin-embedded tissue sections EBS-IF-003 recognizes only CK13. On cryostat sections EBS-IF-003 serves as differentiation-related marker of all stratified epithelia; it stains all suprabasal cells in both cornifying and noncornifying stratified epithelia and more differentiated cells of squamous carcinomas. In paraffin sections EBS-IF-003 does not stain CK10 positive, CK13 negative epithelia, as for example epidermis.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
EBS-IF-003
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Ivanyi D. et al, J. Pathol. 159: 7-12 (1989)
References 2:
Ivanyi D. et al, Am. J. Vet. Res. 53: 304-314 (1992)
References 3:
Ivanyi D. et al, Am. J. Vet. Res. 54: 1095-1102 (1993)
References 4:
Kozaki, M et al, J. Vet. Med. Sci. 63(1): 1-4 (2001)
EBS-IF-004 reacts specifically with keratin 14 (50kDa) in immunoblotting. In tissue sections EBS-IF-004 is positive with basal cells of non-keratinizing stratified epithelia, basal cells and suprabasal cells of the epidermis and gingiva, myoepithelial cells and squamous cell carcinomas.
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
EBS-IF-004
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Ivanyi, D. et al. Am. J. Vet. Res. 53: 304-314 (1992)
References 2:
Ivanyi, D. et al. Cancer Res. 50(16): 5143-5152 (1990)
References 3:
Balm A.J.M. et al., Eur. Arch. Otorhinolaryngol. 253: 227-233 (1996)
E3 reacts with keratin 17 (45 kDa). The antibody reveals myoepithelial cells, basal cells and proliferating epithelia in some benign epithelial tumors as well as malignant carcinomas.
C-04 Reacts with keratin 18 (45kDa) present in a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
C-04
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bártek et al. J. of Pathol. 164: 215-24 (1991)
References 2:
Bártková et al. Neoplasma 38(4): 439-46 (1991)
References 3:
Lauerová et al. Hybridoma 7: 495-504 (1988)
References 4:
Taylor Papadimitriou et.al. J. Cell Sci. 94: 403-13 (1989)
References 5:
Kova?ík et al. J. Tumor Marker Oncol. 5: 219 (1990)
EBS-IF-007 is human specific and reacts with rod domain 2B, aa 311-335, of keratin 19 (40 kDa). Keratin 19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-keratin 19 reacts with a wide variety of corresponding epithelial malignancies.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
EBS-IF-007
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Böttger, V. et al., Eur. J. Biochem. 231: 475-485 (1995)
References 2:
Karsten et al., Eur.J.Cancer Clin. Oncol. 21(6), 733-740 (1985)
References 3:
Kasper et al., Histochemistry 89(4), 369-377 (1988)
C11 reacts with keratins : 4,5,6,8,10,13 and 18. This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
C-11
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Vojt?ek, B. et al. Folia Biol., 35(6): 373-382, (1989)
References 2:
Vojt?ek, B. et al. Neoplasma 37(3): 333-342 (1990)
References 3:
Kova?ík J. et al. Int. J. Cancer, Suppl. 3: 50-55, (1988)
References 4:
Kova?ík J. et al. J. Tumor Marker Oncol. 5: 219 (1990)
LN-6 reacts with vimentin, a 58kDa protein, and specifically with a non-hematopoietic epitope of vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Vimentin is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells, and endothelium. Antibody against vimentin is useful as part of an antibody panel for differential diagnosis of tumors of unknown origin. It does not react with leukocyte common antigen-positive tissues such as lymphomas and leukemias.
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
LN-6
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Stathopoulos, E, et al, J. Histochem. Cytochem. 37: 1363-1370 (1989)
EBS-O-104 reacts with a monomorphic determinant of human major histocompatibility (MHC) class I antigens (HLA-A, B and C). Human MHC class I antigens are expressed constitutively on all nucleated cells lymphocytes such as lymphocytes, thymocytes, granulocytes, and bone marrow cells and also platelets but are absent on erythrocytes. MHC class I antigens play a role in class I MHCassociated antigen presentation, inhibition of NK cell cytotoxicity, tumor surveillance, and tissue allotransplantation.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
EBS-O-104
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Young NT et al. Hum Immunol 52(1): 1-11 (1997)
References 2:
Krensky AM et al, Transplant Proc 28(6): 3026-8 (1996)
References 3:
Hansen JA et al, Hematol Oncol Clin North Am 4(3): 507-515 (1990)
Bra23/9 reacts with a monomorphic determinant of human major histocompatibility (MHC) class I antigens (HLA-A, B and C). Human MHC class I antigens are expressed constitutively on all nucleated cells and platelets and are absent on erythrocytes. MHC class I antigens play a role in class I MHCassociated antigen presentation, inhibition of NK cell cytotoxicity, tumor surveillance, and tissue allotransplantation.
108-2C5 recognizes an intralocus determinant present on a limited number of HLA-A locusencoded gene products (HLA-A2, -A3, A28, -A29, -A30, -A31 and -Aw33). Furthermore, by testing its reactivity with HLA-A2 natural variants and mutants, the importance of amino acid residues 79 and/or 80 of the alpha1 domain was demonstrated in the formation of an intralocus HLA-A determinant.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
108-2C5
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Lozano, F. et al., Immunogenetics 30: 50-53 (1989)
References 2:
Lozano, F. et al., Tissue Antigens 35: 193-195 (1990)
References 3:
Domenech, N. et al., Human Immunol 30: 140-146 (1991)
References 4:
Ryschich, E, et al, Clin Cancer Res. 11(2 Pt 1): 498-504 (2005)
EP-4 recognizes the HLA-B27 cell surface antigen on human cells. HLA-B27 has been found to be highly associated (60%) with ankylosing spondylitis (Bekhterev's disease, MarieStrümpell disease or "bamboo spine"). While rheumatoid factor tests will be negative, testing for the presence of HLA-B27 in a patient can confirm the diagnosis of ankylosing spondylitis. Also postgonococcal arthritis and acute anterior uveitis are associated with HLA-B27.
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
EP-4
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Hansen JA et al. Hematol Oncol Clin North Am, 4(3): 507-515 (1990)
References 2:
El-Shabrawi, Y. et al. Ophthalmology 113: 695-700 (2006)
EBS-O-109 reacts with human ?-2 microglobulin, a 22 kDa protein, which associates noncovalently with the 44kDa ?1-chain of the HLA Class I complex found on all nucleated cells and on platelets. There is no reaction with erythrocytes, neither with non-human primate cells. The detection of ?-2 microglobulin in body fluids has been used as a tumor marker, renal failure marker and for monitoring patients with HIV infection.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
EBS-O-109
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Brodsky FM et al, lmmunol Rev 47: 3-61 (1979)
References 2:
Ryschich, E, et al, Clin Cancer Res. 11(2 Pt 1): 498-504 (2005)
Betts RL, et al. Monoclonal hybridoma antibodies: Techniques and applications. Edited by D. Hurrel. Uniscience series program. C.R.C. Press, Cleveland, OH: (1983), pp. 193-222
MHC class II molecules are encoded by polymorphic MHC genes and consist of a non-covalent complex of an ? and ? chain. Helper T lymphocytes bind antigenic peptides presented by MHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes.
Antibody Isotype:
IgG2b-K
Monosan Range:
MONOSAN
Clone:
BraFB6
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Babusikova O et al., Neoplasma 32(6): 657-62 (1985)
MHC class II molecules are encoded by polymorphic MHC genes and consist of a non-covalent complex of an ? and ? chain. Helper T lymphocytes bind antigenic peptides presented by MHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes.
Antibody Isotype:
IgG3-K
Monosan Range:
MONOSAN
Clone:
Bra14
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Chorvath B et al. Neoplasma 34(4):417-425 (1987)
References 2:
Horejsi V et al. Tissue Antigens 32(1):6-11 (1988)
EBS-O-114 reacts with HLA Class II. The antibody was shown to strongly block cytotoxic activity of T4+ cytotoxic T cell clones. Distribution studies on cell lines suggested that EBS-O-114 is directed against a DQ rather than a DR antigen.
MHC class II molecules are encoded by polymorphic MHC genes and consist of a non-covalent complex of an ? and ? chain. Helper T lymphocytes bind antigenic peptides presented by MHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes.
Antibody Isotype:
IgG2b-K
Monosan Range:
MONOSAN
Clone:
LN-3
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Marder, R.L. et al. Lab. Invest. 52: 497-504 (1985)
References 2:
Andrade, R.E. et al. Human Pathology 19: 932-941 (1988)
References 3:
Azumi N. et al. Human Pathology 19: 1376-1382 (1988)
MHC class II molecules are encoded by polymorphic MHC genes and consist of a non-covalent complex of an ? and ? chain. Helper T lymphocytes bind antigenic peptides presented by MHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes.
Antibody Isotype:
IgG2-K
Monosan Range:
MONOSAN
Clone:
EBS-O-110
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Sparrow RL, et al., Transplantation 42: 647-652 (1986)
References 2:
Chorvath B et al. Neoplasma 34(4): 417-425 (1987)
References 3:
Horejsi V et al. Tissue Antigens 32(1): 6-11 (1988)
MHC class II molecules are encoded by polymorphic MHC genes and consist of a non-covalent complex of an ? and ? chain. Helper T lymphocytes bind antigenic peptides presented by MHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
Bra30
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Chorvath B et al. Neoplasma 34(4): 417-425 (1987)
References 2:
Horejsi V et al. Tissue Antigens 32(1): 6-11 (1988)
CDw78 (also called Ba antigen, Leu21 or LO panB a) is present on some immature and some mature B-cells. The antigen appears on B-cell progenitors preceding CD10, CD19, CD22, and CD37. It is expressed on resting B-cells and reappears and persists in the cytoplasm and on the cell surface until cytoplasmic Ig appears. Its expression is greatly increased after B-cell activation in vitro. It is also found on tissue macrophages and on epithelial cells, but not on T-cells, NK cells, monocytes, granulocytes, thymocytes or bone marrow stromal fibroblasts nor myeloid tissues. 60-3G2 was typed at CD workshop IV.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
60-3G2
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Pinchouk VG. et al, Anticancer Research 8: 1377-1380 (1988)
References 2:
Gluzman DF. et al, Tissue Antigens 33: 151 (1989)
References 3:
Sidorenko SP. et al, Neoplasma 39: 3-9 (1992)
References 4:
Moldenhauer et al, Leucocyte Typing IV, pp 155 162, (1989)
References 5:
Pezzuto et al, Leucocyte Typing IV, pp 165 174, (1989).
CDw78 (also called Ba antigen, Leu21 or LO panB a) is present on some immature and some mature B-cells. The antigen appears on B-cell progenitors preceding CD10, CD19, CD22 and CD37. It is expressed on resting B-cells and reappears and persists in the cytoplasm and on the cell surface until cytoplasmic Ig appears. Its expression is greatly increased after B-cell activation in vitro. It is also found on tissue macrophages and on epithelial cells, but not on Tcells, NK-cells, monocytes, granulocytes, thymocytes or bone marrow stromal fibroblasts nor myeloid tissues.
Antibody Isotype:
IgG3-K
Monosan Range:
MONOSAN
Clone:
IPO-10
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Pinchouk V.G. et al, Anticancer Res. 8: 1377-1380 (1988)
MHC class II molecules are encoded by polymorphic MHC genes and consist of a non-covalent complex of an ? and ? chain. Helper T lymphocytes bind antigenic peptides presented by MHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumulating in endosomal/lysosomal compartments and on the surface of B-cells, HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
EBS-O-111
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Thompson CJ et al., Human Immunol 6: 133-150 (1983)
The CD74 cluster, established during the IVth and Vth Leukocyte Typing Workshops, comprises four species of proteins (MW 41/35/33 kDa), all coded by a single gene, consisting of nine exons. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. In tissue sections anti-CD74 show a binding pattern very similar to that of anti-HLA-DR. It binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. EBS-CD-041 epitope is localized in the extracellular domain of CD74.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-041
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Epstein A.L. et al. J. Immunol. 133: 1028-1036 (1984)
References 2:
Marder, R.J. et al. Lab. Invest. 52: 497-504 (1985)
References 3:
Lazova R. et al. Cancer 79: 2115-2124 (1997).
References 4:
Pich, A. et al. Eur J Basic Appl Histochem. 35(1): 81-9 (1991)
EBS-I-014 reacts with flagellar core protein of Borrelia species including B.burgdorferi. B.burgdorferi is a species of bacteria of the spirochete class of the genus Borrelia causing Lyme disease, a vector-borne, multisystem inflammatory disease which is transmitted to humans by the bite of ticks of the Ixodes ricinus complex
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
EBS-I-014
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Busch, U. et al, J. Clin. Microbiol. 34: 1072-1078 (1996)
EBS-I-001 reacts with a soluble excreted antigen in ELISA. This determinant is unaffected by frozen storage of specimens, unlike antibodies to flagellar antigens which require freshly cultured organisms.
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
EBS-I-001
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Altekruse, SF, at al, Emerg Infect Dis. 5: 28-35 (1999)
EBS-I-002 reacts with a soluble excreted antigen in ELISA. This determinant is unaffected by frozen storage of specimens, unlike antibodies to flagellar antigens which require fresh cultured organism. Positive: C. jejuni, type 1 (K807, K858, K634) Type 2, C. coli, C. hyointestinalis, C lardis. Cross reacts with Staphylococcus aureus and weakly with Pseudomonas fluorescens. Negative: C. fetus, C. fetus intestinals, C. faecalis, H. pylori, Listeria monocytogenes, Actinomyces israelii, E. coli, Lactobacillus casei, Bacillus cereus, C. freundi, Salmonella Virchow, Streptococcus faecalis and Enterobacter aerogenes.
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
EBS-I-002
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Baily E.L. et al. Mol. Ecol 24(1): 208-21 (2015)
References 2:
Haddock G et al. microbiology 156(10): 3079-84 (2010)
References 3:
Altekruse, SF, at al, Emerg Infect Dis. 5: 28-35 (1999)
EBS-I-100 reacts with C. difficile Toxin A, but not with V. cholerae subunit a, V. cholerae toxin, Pseudomonas aeruginosa exotoxin A, H-LT, P-LT. C. difficile is a major nosocomial pathogen that causes antibiotic-associated colitis and mediates inflammatory diarrhea by releasing two large protein enterotoxins (toxin A and toxin B) that are able to disrupt intestinal epithelial cells via their transferase activity and ability to monoglucosylate members of the Rho family. C. difficile toxin A is a toxin that is composed of 39 repeats that are responsible for binding to intestinal epithelial cell surface carbohydrates. C. difficile toxin A causes significant apoptosis of colonocytes which contributes to the formation of ulcers and pseudo-membranes in a pathway that involves p38-dependent activation of p53 and induction of p21, leading to cytochrome c release and caspase-3 activation through Bak activation.
Antibody Isotype:
IgG3-K
Monosan Range:
MONOSAN
Clone:
EBS-I-100
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Kim H, et al, Gastroenterology 129: 1875-1888 (2005)
References 2:
Carter JP, et al, Gut Microbes. 1(1): 5864 (2010)
Ten to forty percent of the patients with acquired immunodeficiency syndrome (AIDS) develop cytomegalovirus (CMV) infections. In some patients with AIDS, CMV is detected in the bronchoalveolar lavage fluid (BALF), urine, and other specimens, even when there are no symptoms of CMV disease. An indicator of active CMV infection is needed to facilitate the diagnosis of CMV disease in patients with AIDS or HIV infection. CMV p65 antigen was detected in the leukocytes of both the peripheral blood and BALF during the early phase of CMV disease.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
CMV221
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Hanabusa H, et al, Kansenshogaku Zasshi. 68(9): 1105-12 (1994)
Ten to forty percent of the patients with acquired immunodeficiency syndrome (AIDS) develop cytomegalovirus (CMV) infections. In some patients with AIDS, CMV is detected in the bronchoalveolar lavage fluid (BALF), urine, and other specimens, even when there are no symptoms of CMV disease. An indicator of active CMV infection is needed to facilitate the diagnosis of CMV disease in patients with AIDS or HIV infection. CMV p65 antigen was detected in the leukocytes of both the peripheral blood and BALF during the early phase of CMV disease
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
CMV-224
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Hanabusa H, et al, Kansenshogaku Zasshi. 68(9): 1105-12 (1994)
Ten to forty percent of the patients with acquired immunodeficiency syndrome (AIDS) develop cytomegalovirus (CMV) infections. In some patients with AIDS, CMV is detected in the bronchoalveolar lavage fluid (BALF), urine, and other specimens, even when there are no symptoms of CMV disease. An indicator of active CMV infection is needed to facilitate the diagnosis of CMV disease in patients with AIDS or HIV infection. CMV p65 antigen was detected in the leukocytes of both the peripheral blood and BALF during the early phase of CMV disease.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
CMV-227
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Hanabusa H, et al, Kansenshogaku Zasshi. 68(9): 1105-12 (1994)
EBS-I-024 reacts with a conserved domain on EBNA1 (AA 430-442), present in all EBV isolates. EBNA1 is expressed in all viral latency stages in vitro and in vivo.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-I-024
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Chen, MR et al. J. Virol. 67(8): 4875-85 (1993)
References 2:
Chen, MR et al. J. Biomed. Sci. 5(3): 173-9 (1998)
References 3:
Sivachandra, N, et al, J. Virol. 86(1): 60-68 (2012)
EBS-I-025 reacts with a conserved repeat domain on LMP1 (AA 268-286), present in all EBV isolates. LMP1 is expressed in most viral latency stages in vitro and in vivo
Giardiasisis a diarrheal illness caused by a single celled microscopic protozoan parasite, Giardia lamblia, also known as Giardia intestinalis. Giardia lamblia exists in two forms, an active form called a trophozoite, and an inactive form called a cyst. The active trophozoite attaches to the lining of the small intestine and is responsible for causing the signs and symptoms of giardiasis. The trophozoite cannot live long outside of the body and spread of infection is via the cyst, which is excreted in the host's feces. When it is ingested, stomach acid activates the cyst, and the cyst develops into the disease causing trophozoite in the new host. Giardiasis is diagnosed by finding cysts or trophozoites in the feces.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-I-039
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Misra, V, et al, Indian J. Pathol. Microbiol. 49: 519-523 (2006)
Membrane fusion is mediated by envelope glycoproteins for enveloped viruses like herpes simplex. Four of at least 10 viral glycoproteins are necessary and sufficient to facilitate fusion of herpes simplex to target cells. These four glycoproteins include glycoprotein B (gB), glycoprotein D (gD), glycoprotein H (gH) and glycoprotein L (gL). Fusion is dependent upon the expression of a gD receptor on target cell membranes.postive: HSV-1, Negative HSV-2.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
EBS-I-042
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bystricka, M, et al, Acta Virol. 43: 399-402 (1999)
Monoclonal antibody HPV-13E2 reacts with HPV-16 E6 peptide (MHQKRTAMFQDPPQERPRKLPQLC). Infection with specific types of HPV has been associated with an increased risk of developing cervical neoplasia. HPV types 6 and 11 have been associated with relatively benign diseases such as genital warts but types 16 and 18 are strongly associated with cervical, vaginal, and vulvar malignancies
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
HPV-13E2
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Beldermann, F. Virusprotein E6 des humanen Papillomavirus HPV-16, Thesis, University of Heidelberg, Germany (1995).
Monoclonal antibody HPV-4B12 reacts with HPV-16 E6 peptide (MHQKRTAMFQDPPQERPRKLPQLC). Infection with specific types of HPV has been associated with an increased risk of developing cervical neoplasia. HPV types 6 and 11 have been associated with relatively benign diseases such as genital warts but types 16 and 18 are strongly associated with cervical, vaginal, and vulvar malignancies.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
HPV-4B12
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Beldermann, F. Virusprotein E6 des humanen Papillomavirus HPV-16, Thesis, University of Heidelberg, Germany (1995).
Monoclonal antibody HPV-4G3 reacts with HPV-18 E6 peptide. Infection with specific types of HPV has been associated with an increased risk of developing cervical neoplasia. HPV types 6 and 11 have been associated with relatively benign diseases such as genital warts but types 16 and 18 are strongly associated with cervical, vaginal, and vulvar malignancies.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
HPV-4G3
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Beldermann, F. Virusprotein E6 des humanen Papillomavirus HPV-16, Thesis, University of Heidelberg, Germany (1995).
EBS-T-001 reacts with BCL10. Having an N-terminal caspase recruitment domain (CARD), BCL10 can induce apoptosis and activate NF-?B. It is found on subpopulations of normal B and T cells, and is associated with MALT1, a paracaspase that, like BCL10, can be found translocated in MALT lymphoma. In such cases either BCL10 or MALT1 or both are highly expressed, depending on the site of translocation. MALT lymphomas lacking this translocation exhibit much lower levels of expression. BCL10 has been shown to be functionally conserved all the way back to zebrafish
BLA36 is a developmentally regulated 36 kDa antigen expressed on the plasma membrane of B lymphocytes, Reed-Sternberg, and mononuclear Hodgkins cells. It also gives strong staining of B cell lymphomas including follicular center cell lymphomas (large and small cell types), mantle zone lymphomas, and immunoblastic lymphomas.
MoBU-1 is reactive with cells containing incorporated 5-bromodeoxyuridine (BrdU) showing a clear, nucleus confined, speckled pattern. BrdU is an analogue of thymidine and can be introduced to proliferating cells in vitro or in vivo, which in turn incorporate BrdU into the DNA during S phase, prior to cell division. Immunocytochemical staining with MoBU-1 will show the degree of proliferation, detecting nucleated cells which have incorporated BrdU in place of thymidine into their DNA.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
MoBU-1 (85-2C8)
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Harms et al. Acta Histochemica, Suppl. Band 36, 353-359 (1988)
EBP-333 reacts with C/EBP? or CCAAAT/enhancer-binding protein beta, a transcription factor which is not only critical for normal macrophage functioning and differentiation, but affects a variety of other factors as well, such as cytokines (IL-6; IL-4; IL-5 and TNF-alpha), neurotransmitters and other neuronal factors and processes like muscle repair and the development of multi drug resistance in tumors (P-glycoprotein). Observations have implied that manipulation of the transcription factors involved may make it possible to modulate multidrug resistance, while leaving normal function of P-glycoprotein intact.
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
EBP-333
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Couturier, C et al, Arterioscler Thromb Vasc Biol. 20(12): 2559-65 (2000)
121SLE reacts with CA19-9 (>400 kDa) or sialyl Lea structure, which is synthesized from type 1 blood group precursor chains and is present in individuals expressing the Lea and/or Leb blood group antigens. 121SLE also binds to some extend to the afuco version of SLe a (LSTa; CA50). In normal tissues, CA19-9 is present in ductal epithelium of the breast, kidney, salivary, gland and sweat glands. Its expression is greatly enhanced in serum as well as in the majority of tumor cells in gastrointestinal (GI) carcinomas, including adenocarcinomas of the stomach, intestine and pancreas. 121SLE was typed in the ISOBM TD-6 workshop.
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
121SLE
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Rye PD. et al, Tumor Biol 19 (5): 390-420, (1998).
References 2:
Christopher MG. et al, Cytojournal 8: 7 (2011)
References 3:
Rouger et al. Blood transfusion and immunohematol. 30(5): 353-720 (1987)
4D11 reacts with CRASH, a 308 AA glycoprotein, sharing 32% homology with human asparaginase and identical to Asparaginase-Like Protein (ALP) found in rat sperm. CRASH is found in a variety of tumors but only occasionally in normal tissues. Especially ovarian cancers are highly positive. CRASH is further found in brain tumors, some colonic cancers, some lung cancers, some prostate cancers, uterine cancers, some breast cancers and some thyroid cancers. It was not found in serum. In Western blot 3 bands are demonstrated of 45 kDa, 28 kDa and 15 kDa.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
4D11
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Weidle, UH et al, Anticancer Res. 29(4): 951-63 (2009).
7B6 reacts with CRASH, a 308 AA glycoprotein sharing 32% homology with human asparaginase and identical to Asparaginase-Like Protein (ALP) found in rat sperm. CRASH is found in a variety of tumors but only occasionally in normal tissues. Especially ovarian cancers are highly positive. CRASH is further found in brain tumors, some colonic cancers, some lung cancers, some prostate cancers, uterine cancers, some breast cancers and some thyroid cancers. It was not found in serum. In Western blot 3 bands are demonstrated of 45 kDa, 28 kDa and 15 kDa.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
7B6
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Weidle, UH et al, Anticancer Res. 29(4): 951-63 (2009).
IPO-38 reacts with a 12-14 kDa protein, as found in Western blots of Raji cells, and appears in the mitotic cycle earlier than Ki-67. Lymphocytes, induced to early G1 phase by 12h exposure to PHA, will become positive while non-stimulated lymphocytes remain negative. Mononuclear cells and granulocytes of healthy donors are negative, while various forms of leukemia and lymphoma including Hodgkins disease are positive for IPO-38, as are many solid tumors such as some breast, gastric and colonic cancers for which it may serve as tumor progression marker.
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
IPO-38
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Sidorenko, S.P. et al., Gematol. Transfuziol. 35: 19-22 (1990)
References 2:
Sidorenko, S.P. et al., Experem. Oncol. 16: 145-150 (1994)
References 3:
Thosaporn W., et al., Oral Dis. 10(1): 22-6 (2004)
References 4:
Hao Y. et al.,J Proteome Res. Sep;7(9): 3668-77 (2008)
References 5:
Makohon N.V. et al., Fiziol Zh. 54(6): 49-57 (2008)
MFG-06 reacts with a glycoprotein of 40-45 kDa, known as Milk fat globule-EGF factor 8 protein (MFG-E8), lactadherin, p47 or milk fat globule 1 antigen. It is present on normal epithelial cells in various organs and considered a differentiation marker in carcinomas. It contains one EGF-like domain and 2 F5/8 type C domains. Functioning as a specific ligand for Integrin ?5 and Integrin ?3, MFG-E8 is thought to be involved in gamete interactions and cell attachment, possibly playing a role in fertilization and apoptosis. Additionally, MFG-E8 binds to rotavirus and inhibits its replication, thereby protecting the cell from viral infection. Overexpression of MFG-E8 is associated with breast cancer, suggesting that MFG-E8 may be related to tumorigenesis or progression. Among testicular carcinomas, seminomas are negative.
EBS-C-003 recognizes Ku protein or XRCC5/6 (X-ray repair cross complementing protein 5/6), involved in Pol II-directed transcription by virtue of its DNA binding activity, serving as the regulatory component of the DNA-associated protein kinase that phosphorylates Pol II and transcription factor Sp. Ku proteins also activate transcription from the U1 small nuclear RNA and the human transferrin receptor gene promoters. It serves as autoantigen in patients with rheumatic diseases.
EBS-T-005 reacts with MADER or Melanoma-Associated Delayed Early Response protein, encoded by the NAB2 gene, belonging to the family of NGFI-A binding proteins. They modulate transcription induced by some members of the EGR (early growth response) family of transactivators. NAB proteins can form homo- or hetero-multimers with other EGR or NAB proteins through a conserved N-terminal domain, and repress transcription through two partially redundant C-terminal domains.
EBS-T-006 reacts with MADER or Melanoma-Associated Delayed Early Response protein, encoded by the NAB2 gene, belonging to the family of NGFI-A binding proteins. They modulate transcription induced by some members of the EGR (early growth response) family of transactivators. NAB proteins can form homo- or hetero-multimers with other EGR or NAB proteins through a conserved N-terminal domain, and repress transcription through two partially redundant C-terminal domains.
EBS-T-007 reacts with paracaspase MALT1, crucial for B- and T-cell activation/proliferation and activation of transcription factor NF-?B. It has an N-terminal death domain, two central immunoglobulin-like domains involved in the binding to the B-cell lymphoma 10 (BCL10) protein and a caspase-like domain. MALT1 and BCL10, can both be found translocated in MALT lymphoma. In such cases either BCL10 or MALT1 or both are highly expressed, depending on the site of translocation. Normal cells and MALT lymphomas lacking translocations exhibit much lower levels of expression.
47-8D3 reacts with macrophages and detects the well-known leukocyte L1, cystic fibrosis antigen. Detecting a single protein band of 14 kDa in Western blots of lysates of human monocytes and granulocytes, the antigen was identified as the calcium-binding protein MRP14, which is a member of the S100 family involved a.o. in regulating the cell cycle. MRP14 is also implicated in the abnormal differentiation of myeloid cells in the stroma of cancer. It is further found on squamous mucosal epithelia. When associated with MRP8 it forms the heterodimer calprotectin.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
47-8D3
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Flavell DJ. et al., J. Histochem. Cytochem. 35: 1217-1226 (1987)
References 2:
Facchetti F. et al., Am. J. Clin. Pathol. 92: 42-50 (1989)
References 3:
Bardadin KA. et al., J. Pathol. 164: 253-259 (1991)
References 4:
Goebeler M. et al., J. Leukocyte Biol. 55: 259-261 (1994)
POH-1 detects the three bands within the 34kDa region corresponding to the p34 protein cyclin dependent kinase 1 (cdk1) and its cleavage products. It is positive in immunoblotting on HeLa cell lysate and negative on fibroblast (LEP strain) cell lysate. A cdc2 homolog, the cdk2 protein kinase, does not react with POH-1. Activated cdk1 / p34cdc2 performs specific functions during mitosis, including nuclear envelope breakdown and chromosome condensation.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
POH-1
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Luká, J, et al. Eur. J. Biochem., 207: 169-176 (1992)
Bp53-12 reacts with an N-terminal epitope (aa 16-25) of both wild-type and mutated p53. This epitope is revealed in tissue sections only after formalin fixation. Mutation and/or allelic loss of p53 is one of the causes of a variety of mesenchymal and epithelial tumors. p53 Localizes in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution.
Antibody Isotype:
IgG2a-A
Monosan Range:
MONOSAN
Clone:
Bp53-12
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bártek J. et al, J Pathol. 169(1):27-34 (1993)
References 2:
Vogelstein and Kinzler, Cell 70: 523-526, (1992)
References 3:
Hollstein et al, Science 253: 49-53: (1991)
References 4:
Lane, D.P, Nature 358: 15-16: (1992)
References 5:
Donehower et al, Biochemic. Biophys. Acta 1155: 181-182, (1993)
EBS-T-059 recognizes an oncofetal antigen of 220kDa, identified as a tumor-associated glycoprotein (TAG72) with properties of a mucin and usually expressed by adenocarcinomas, but not by mesotheliomas and thus helps in the differential diagnosis of malignancies of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma. TAG72 is further used as serum marker for gastric cancer.
MAPKs are involved in directing cellular responses to a diverse array of potentially harmful stimuli, such as mitogens, osmotic stress, heat shock, proinflammatory cytokines, but also growth factors (mammals). Possibly located exclusively in the cell nucleus, they regulate cell functions including proliferation, gene expression, differentiation, mitosis, cell survival, and apoptosis. In order to become active, they require usually multiple phosphorylation events in their activation loops, including phosphorylation by MAP2 kinases (Ste-7 kinases), which in turn are phosphorylated by the MAP3 kinase family, of which many are located at the cell membrane. Thus through this pathway, stimuli can effectively be conveyed from the cell membrane to the nucleus. Inactivation of MAPKs takes place by several phosphorylases, including dedicated phophorylases.
EP-4 recognizes the HLA-B27 cell surface antigen on human cells. HLA-B27 has been found to be highly associated (60%) with ankylosing spondylitis (Bekhterev's disease, MarieStrümpell disease or "bamboo spine"). While rheumatoid factor tests will be negative, testing for the presence of HLA-B27 in a patient can confirm the diagnosis of ankylosing spondylitis. Also postgonococcal arthritis and acute anterior uveitis are associated with HLA-B27.
Antibody Isotype:
IgM,kappa
Monosan Range:
MONOSAN
Clone:
EP-4
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Hansen JA et al. Hematol Oncol Clin North Am, 4(3): 507-515 (1990)
References 2:
El-Shabrawi, Y. et al. Ophthalmology 113: 695-700 (2006).
IPO-M6 reacts with human leukemia cell line HL-60 and immuno-precipitates two proteins with MW of 48 and 52 kDa. IPO-M6 does not stain B cell lines Daudy, PHS, Namalwa, RPMI-1788 and T-cell lines CCRF-HSB2, Jurkat and Molt-4. IPO-M6 can be applied for staining of monocytes and up to 10 % of lymphocytes from peripheral blood of healthy donors. Blast cells of patients with AMMonL (M5 following FAB classification), AMMonL (M4) and hairy cells leukemia are IPO-M6 positive. The antigen, defined by IPO-M6, is particularly expressed on blood cells from patients with infectious mononucleosis and CLL. Histiocytes and macrophages are also positive. Malignant cells from patients with AML (M1 and M2), T-ALL, B ALL are IPO-M6 negative.
A9E4 reacts with GnRH receptors in the anterior pituitary. GnRH stimulates the gonadotrophs of the anterior pituitary to secrete luteinising hormone (LH) as well as follicle-stimulating hormone (FSH). The receptor contains seven hydrophobic transmembrane domains connected by hydrophilic extracellular, and intracellular loops, characteristic of G-protein coupled receptors. Some cancers like ovarian and breast cancers sometimes carry GnRH receptors
Antibody Isotype:
IgG1,kappa
Monosan Range:
MONOSAN
Clone:
A9E4
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Karande, AA, et al, Molec. Cell. Endocrinol. 114: 51-56 (1995)
58-15 Recognizes riboncleoproteins (RNP), found predominantly in nuclear ribonucleoprotein (nRNP) particles, one of the main components of nucleoli. It identifies cells active in the cell cycle and hence can be used to measure the mitotic activity of cell populations. Since the antibody can be used in paraffin embedded tissue sections, it can identify actively cycling cells within routinely fixed tissue specimens. 58-15 Can be considered a pan nRNP antibody. Pan nRNP antibodies provide detection for a range of RNP proteins.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
58-15
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Clevenger, C.V. et al. J Histochem Cytochem 32: 757-765 (1984)
References 2:
Clevenger, C.V. et al. Cytometry 6: 208-214 (1985)
References 3:
Maryam A and Nigel WF, J Virol. 75: 12070-12080, (2001)
F1G4 reacts with GnRH receptors in the anterior pituitary. GnRH stimulates the gonadotrophs of the anterior pituitary to secrete luteinising hormone (LH) as well as follicle-stimulating hormone (FSH). The receptor contains seven hydrophobic transmembrane domains connected by hydrophilic extracellular, and intracellular loops characteristic of G protein couple receptors. Some cancers like ovarian and breast cancers sometimes carry GnRH receptors.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
F1G4
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Karande, A.A., et al., Molec. Cell. Endocrinol. 114: 51-56 (1995)
TU-02 reacts with the N-terminal domain of alpha-tubulin. Tubulin isotypes have been identified with tissue specific expression. Immunocytochemical studies using several mAbs revealed remarkable heterogeneity of tubulin within various nervous tissues. TU-02 reacts with tubulin in fixed tissues only, not in unfixed or live tissues or cells. Interphase microtubules are also stained by TU-02 in fixed tissues.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
TU-02
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Dráber, P. et. al. Eur.J.Cell.Biol. 41: 82-88 (1986)
References 2:
Dráber, P. et. al. Histochemistry 87: 151-155 (1987)
References 3:
Dráber, P. et. al. J. Cell Science 92: 519-528 (1989)
References 4:
Smertenko et al. Eur. J. Cell. Biol. 72: 104-112 (1997)
MBS-12 specifically detects AFP. This protein is one of the major serum proteins in the early life of mammals and through to be fetal counterpart of albumin. AFP production is reactivated in the adult during liver regeneration and hepatocarcinogenesis, though in some individuals it persists into adulthood naturally. It is positive on all yolk sac tumors, on some other germ cell tumors and on hepatocellular carcinomas.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MBS-12
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Stefanova, I. et al, J. Immunol. Methods. 111: 67-73 (1988)
C2 has been characterized in the ISOBM TD-2 workshop and assigned by K. Nustad to group E of a cluster of 6 major epitopes of human alpha fetoprotein. Human alpha fetoprotein is an oncofetal protein of 70 kDa. It is expressed in fetal liver and is normally absent in health adult tissues. It is positive on all yolk sac tumors, on some other germ cell tumors and on hepatocellular carcinomas.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
C2
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Tsung K., et al. J. Immunol. Methods 39: 363-368 (1980)
References 2:
Michell B.et al. Eur. J. Cancer Clin. Oncol. 19: 1239-1246 (1983)
References 3:
Yazova A.K. et al. Immunol. Lett. 25: 325-330 (1990)
References 4:
Nustad K. Et al. Tumor Biol 19: 293 -300 (1998)
References 5:
Yakimenko E.F. et al. Tumor Biol 19: 301-309 (1998)
C3 has been characterized in the ISOBM TD-2 workshop and assigned by K. Nunstad to a group of antibodies with low affinity to human alpha fetoprotein. Human alpha fetoprotein is an oncofetal protein of 70 kDa and expressed in fetal liver but normally absent in healthy adult tissues. It is expressed in all yolk sac tumors, in some other germ cell tumors and in hepatocellular carcinomas.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
C3
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Tsung K., et al. J. Immunol. Methods 39: 363-368 (1980)
References 2:
Michell B.et al. Eur. J. Cancer Clin. Oncol. 19: 1239-1246 (1983)
References 3:
Yazova A.K. et al. Immunol. Lett. 25: 325-330 (1990)
References 4:
Nustad K. Et al. Tumor Biol 19: 293 -300 (1998)
References 5:
Yakimenko E.F. et al. Tumor Biol 19: 301-309 (1998)
D10 has been characterized in the ISOBM TD-2 workshop and assigned by K. Nustad to group D of a cluster of 6 major epitopes of human alpha fetoprotein. Human alpha fetoprotein is an oncofetal protein of 70 kDa. It is expressed in fetal liver and is normally absent in healthy adult tissues. It is positive on all yolk sac tumors, on some other germ cell tumors and on hepatocellular carcinomas.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
D10
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Tsung K., et al. J. Immunol. Methods 39: 363-368 (1980)
References 2:
Michell B.et al. Eur. J. Cancer Clin. Oncol. 19: 1239-1246 (1983)
References 3:
Yazova A.K. et al. Immunol. Lett. 25: 325-330 (1990)
References 4:
Nustad K. Et al. Tumor Biol 19: 293 -300 (1998)
References 5:
Yakimenko E.F. et al. Tumor Biol 19: 301-309 (1998)
Pen 9 reacts mainly with the thiazolidin ring of penicillin, but not with the lactam ring. The nature of the side chain in the penicilloyl group does not affect antibody binding as was shown by testing Pen 9 against benzylpenicillin, ampicillin, amoxillin and 6-aminopenicillanic acid. The presence of carrier protein is not essential for the presentation of the antigen associated with Pen 9.
Antibody Isotype:
IgG1-kappa
Monosan Range:
MONOSAN
Clone:
Pen 9
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
P. de Haan et al., Int. Archs Allergy appl. Immun. 76: 42-46 (1985)
PN-15 reacts with a lectin receptor like glycoprotein of 200 kDa (gp200), present in proximal renal tubules and on urothelium. The antigen is carbohydrate in nature. Other normal tissues that display the antigen include breast, parathyroid glands, thymus and epididymis. Among renal carcinomas 93% of primary and 84% of metastatic carcinomas are positive. Bladder cancers are also largely positive. Other tumor types include breast cancer, teratocarcinomas and parathyroid adenomas. The antigen, also called DEC-205, was assigned to CD205 at CD workshop VII. In the immune system it can facilitate tolerance to self-antigens through uptake of apoptosis derived material by dendritic cells, which in turn present fragments through MHC II and MHC I, either inducing or repressing immune responses, depending on the nature of concomitant signals.
Antibody Isotype:
IgG2b-kappa
Monosan Range:
MONOSAN
Clone:
PN-15
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Yoshida, S.O. et al, Cancer Res 49: 1802-1809 (1989)
References 2:
Li, G, et al, Anticancer Res. 20(4): 2773-8 (2000)
References 3:
Batchelder C.A. et al, Anat Rec (Hoboken) 297(8): 1392-1406 (2014)
References 4:
Cykowski M.D. et al, Ultrastruct Pathol 39(1): 69-77 (2015)
The alpha interferons are involved in virus resistance in target cells for these viruses. They are known to block cell proliferation and to regulate MHC class I antigen expression. The IFN? family has over 20 genes and pseudogenes in two families (I and II), one with a mature length of 166aa and one of 172aa. Cells producing IFN? are lymphocytes, monocytes, macrophages and cell lines such as Namalwa and KGI. Bioassays for IFN? include cytopathic effect blocking, by viruses such as VSV, SFV and BMCV, on their target cells. A number of receptors for IFN? are now known and seem to be expressed on most cell types. N27 is specific for human IFN?2 and does not cross react with human IFN?1. N27 reacts with linear peptide 43aa-53aa, placing the epitope outside the immunodominant regions I and II.
Antibody Isotype:
IgG1,kappa
Monosan Range:
MONOSAN
Clone:
N27
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Kontsek, P. et al., Mol Immunol. 29: 863-870 (1992)
References 2:
Kontsek, P. et al., Immunol. Lett. 35: 281-284 (1993)
The alpha interferons are involved in virus resistance in target cells for these viruses. They are known to block cell proliferation and to regulate MHC class I antigen expression. The IFN? family has over 20 genes and pseudogenes in two families (I and II), one with a mature length of 166aa and one of 172aa. Cells producing IFN? are lymphocytes, monocytes, macrophages and cell lines such as Namalwa and KGI. Bioassays for IFN? include cytopathic effect blocking, by viruses such as VSV, SFV and BMCV, on their target cells. A number of receptors for IFN? are now known and seem to be expressed on most cell types. 2-48 Is specific for human interferon ? 1 and does not cross react with human interferon ? 2.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
Feb-48
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Kontsek, P. et al., Mol Immunol. 29: 863-870 (1992)
The alpha interferons are involved in virus resistance in target cells for these viruses. They are known to block cell proliferation and to regulate MHC class I antigen expression. The IFN? family has over 20 genes and pseudogenes in two families (I and II), one with a mature length of 166aa and one of 172aa. Cells producing IFN? are lymphocytes, monocytes, macrophages and cell lines such as Namalwa and KGI. Bioassays for IFN? include cytopathic effect blocking, by viruses such as VSV, SFV and BMCV, on their target cells. A number of receptors for IFN? are now known and seem to be expressed on most cell types. 2-52 Is specific for human IFN?1 and does not cross react with human IFN?2.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
Feb-52
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Kontsek, P. et al., Mol Immunol. 29: 863-870 (1992)
Insulin is a protein consisting of an ?-chain of 21 amino acids and a ?-chain of 30 amino acids and produced in the ?-cells of the pancreas. E2-E3 is a specific insulin antibody as tested by ELISA and on human pancreatic tissue.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
E2-E3
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
De la Tour, D, et al, Mol. Endoc. 15: 476-483 (2001)
References 2:
Rajagopal, J, et al, Science 299: 363 (2003)
References 3:
Morisset, J, et al, J. Histochem. Cytochem. 51: 1501-1513 (2003)
The alpha interferons are involved in virus resistance in target cells for these viruses. They are known to block cell proliferation and to regulate MHC class I antigen expression. The IFN? family has over 20 genes and pseudogenes in two families (I and II), one with a mature length of 166aa and one of 172aa. Cells producing IFN? are lymphocytes, monocytes, macrophages and cell lines such as Namalwa and KGI. Bioassays for IFN? include cytopathic effect blocking, by viruses such as VSV, SFV and BMCV, on their target cells. A number of receptors for IFN? are now known and seem to be expressed on most cell types. N39 is specific for human IFN?2 and does not cross react with human IFN?1. N39 is directed against immunodominant epitope site I (aa112-148).
Antibody Isotype:
IgG1,kappa
Monosan Range:
MONOSAN
Clone:
N39
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Kontsek, P. et al., Mol Immunol. 29: 863-870 (1992)
References 2:
Kontsek, P. et al., Immunol. Lett. 35: 281-284 (1993)
Insulin is a protein consisting of an ?-chain of 21 amino acids and a ?-chain of 30 amino acids and produced in the ?-cells of the pancreas. 2D11-H5 is a specific insulin antibody as tested by ELISA and on human pancreatic tissue.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
2D11-H5
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
De la Tour, D, et al, Mol. Endoc. 15: 476-483 (2001)
References 2:
Rajagopal, J, et al, Science 299: 363 (2003)
References 3:
Morisset, J, et al, J. Histochem. Cytochem. 51: 1501-1513 (2003)
AE-2 recognizes double stranded DNA expressed in the nucleus of eukaryotes. Dilution advice: Flow cytometry (1-2 µg/million cells in 0,1 ml, fix cells in 4% PFA for 10 min, at 4°C, permeabilize with 0,2% saponin or digitonin for 15 min, at 4°C). ? Immunofluorescence (1-2 µg/ml). ? Immunohistology (1-2 µg/ml for 30 min at RT; staining of formalin-fixed tissues requires incubating tissue sections in 4N HCl, for 30 minutes).
Antibody Isotype:
IgG3
Monosan Range:
MONOSAN
Clone:
AE-2
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Epstein, AL et al. In Progress on nonhistone protein research. 1: 117-137 (1987)
FR4D11 reacts with high affinity to CD10 or CALLA, a cell surface enzyme with neutral metalloendopeptidase activity, inactivating a variety of biologically active peptides. CD10 is a 100 kDa glycoprotein, expressed on 70% of pre-B ALL-cells (common ALL), but also on early lymphoid progenitor-cells in bone marrow and fetal liver. Other normal CD10 positive tissues include renal epithelium, fibroblasts and germinal centre B-cells. Density of CD10 antigen has been shown to be related to cell differentiation and may have prognostic value for B-cell lineage acute leukemia. CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
FR4D11
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Brown, B. et al., J. Natl. Canc. Inst,.55: 1281-1289 (1975)
References 2:
Tran-Paterson, R. et al., Blood, 76: 775-782 (1990)
References 3:
Doerken, B. et al., in Knapp, W. et. al. (eds)., Leucocyte Typing IV, Oxford Univ. Press, pp 33-34
Integrin ?M (also designated complement component receptor 3 ?-chain; CD11b (p170); macrophage antigen ? polypeptide; cell surface glycoprotein Mac-1 ?-subunit; CR3 ?-chain; MAC1A; MO1A and ITGAM) is a cell adhesion molecule that acts as a receptor for cell surface ligands such as intracellular adhesion molecules (ICAMs) or soluble ligands. Integrins are heterodimeric proteins that contain an ?-chain and a ?-chain. Integrin ?M combines with Integrin ?2 (CD18) to form a leukocyte-specific integrin referred to as macrophage receptor-1 (Mac-1) or inactivated-C3b (iC3b) receptor 3 (CR3). Integrin ?M-?2 is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-010
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Beekhuizen H, et al., J Immunol. 145(2):510-8 (1990)
References 2:
Argenbright LW et al., J Leukoc Biol. 49(3):253-7 (1991)
References 3:
Zhou L, et al. J Biol Chem. 269(25):17075-9 (1994)
References 4:
Miller LJ, et al. J Immunol. 137(9):2891-900 (1986)
Integrin ?X (CD11c, leukocyte surface antigen p150/95, CR4, Axb2) is a type 1 transmembrane protein that traditionally combines with ?2 chain to form a leukocyte-specific integrin known as inactivated-C3b (iC3b) receptor 4 (CR4). Integrin ?X/?2 shares similar properties of the Integrin ?M/?2 in mediating adherence of neutrophils and monocytes to stimulated endothelial cells and in phagocytosis of complement coated particles. Abnormal expression of Integrin ?X is characteristic of hairy cell leukemia (HCL) and is dependent upon activation of proto-oncogenes Ras and JunD. Integrin ?x is present on dendritic cells, macrophages and NK-cells. Upon activation, DCs present in skin (Langerhans cells_, lining of nose, lung, stomach, intestine and blood can migrate to lymphoid tissues and interact with T and B-cells to initiate and shape the immune response.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-011
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Cabañas C, et al., Hybridoma 7(2):167-76 (1988)
References 2:
Cabañas C, et al., Immunol Lett. 20(3):193-76 (1988)
References 3:
Zhou JQ, et al. Blood 82:800-6 (1993)
References 4:
Nicolaou, F., et al. Blood 101: 4033-4041 (2003)
References 5:
Edwards, A.D. et al. J. Immunol. 171: 47-60 (2003)
EBS-CD-12 recognizes an extracellular epitope on an integral membrane glycoprotein of 150 kDa, identified as CD13 (also known as aminopeptidase-N). CD13 is present on most cells of myeloid origin including granulocytes, monocytes, mast cells, and GM-progenitor cells. It is also expressed by the majority of AML, CML in myeloid blast crisis, and in a smaller fraction of lymphoid leukemias. CD13 is also present on fibroblasts; endothelial cells, epithelial cells from renal proximal tubules and intestinal brush border, bone marrow stromal cells, osteoclasts, and cells lining bile duct canaliculi. CD13 plays a role in metabolism of biologically active peptides, in phagocytosis, and in bactericidal/tumoricidal activities. It also serves as a receptor for human coronaviruses (hCoV) and human cytomegalovirus (hCMV).
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-012
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Favaloro EJ, et al., Exp Hematol. 13:1695-701 (1993)
References 2:
Favaloro EJ, et al., Clin Chim Acta.220(1):81-90 (1993)
References 3:
Lachance C., et al. J Virol. 72(8):6511-9. (1998)
References 4:
Koch AE, et. al. Am J Pathol. 138(1): 165-73.( 1991)
References 5:
Principe, S. et al, Proteome Res. 6;11(4): 2386-96 (2012)
EBS-CD-013 specifically recognizes CD16. This molecule also named low affinity Fc-receptor for IgG (FcgammaRIII) exhibits two truncated Ig-like domains and is 50-80 kDa. It is highly expressed on NK-cells, granulocytes and macrophages. EBS-CD-013 binds to 15% peripheral lymphocytes of healthy donors (NK-cells), granulocytes, macrophages. CD16 represents the functional receptor structure for antibody-dependent cellular cytotoxicity.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-013
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Deaglio S. et al., Blood 99(7): 2490-8 (2002)
References 2:
Zilber MT et al. Proc Natl Acad Sci U S A 97(6): 2840-5 (2000)
References 3:
Wirthmueller U et al. J Exp Med. 175(5): 1381-90 (1992)
CB16 specifically recognizes CD 16. This molecule also named low affinity Fc-receptor for IgG (FcgammaRIII) exhibits two truncated Ig-like domains and is 50-80 kDa. It is highly expressed on NK-cells, granulocytes and macrophages. CB16 binds to 15% peripheral lymphocytes of healthy donors (NK-cells), granulocytes, macrophages. CD16 represents the functional receptor structure for antibody-dependent cellular cytotoxicity
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
CB16
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Deaglio S. et al., Blood 99(7): 2490-8 (2002)
References 2:
Zilber MT et al. Proc Natl Acad Sci U S A 97(6): 2840-5 (2000)
References 3:
Wirthmueller U et al. J Exp Med. 175(5): 1381-90 (1992)
CDw17 is an intermediate glycosphingolipid from the metabolism of higher gangliosides that localizes to sphingolipid-sterol rafts. CDw17 is found on monocytes, granulocytes, basophils, platelets, a subset of peripheral B-cells (CD19+) and tonsil dendritic cells. It is rapidly down regulated on activated granulocytes and is upregulated on IL-2 activated T-lymphocytes. CDw17 binds to bacteria and may function in phagocytosis. It may also be involved in angiogenesis. Aberrant levels of glycosphingolipids are a feature of cancer cells and may influence integrin clustering and internalization.
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-014
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Knapp W. Leukocyte Typing IV, Oxford Univ. Press, (1989)
It recognizes a transmembrane glycoprotein of 95 kDa, identified as CD18 or intergrin-2 (Workshop III). It complexes non-covalently with either L, M, or X integrin (CD11a, b, or c) to form the heterodimers. LFA-1, MAC-1, and p150,95, respectively. LFA-1 is the receptor for three members of the Ig supergene family of proteins, ICAM-1 (CD54, ICCAM-2 (CD102), and Mac-1 and p150,95 bind to ICAM-1, fibrinogen, and IC3b. ICAM-3 (CD50). CD18/CD11 heterodimeric molecules are involved with cell/cell and cell/extracellular adhesion in immune and inflammatory responses. This Mab blocks these cellular interactions. 68-5A5 was clustered at the IIIrd International Workshop on human leucocyte differentiation antigens.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
68-5A5
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
McMichael A.J.et al., Leucocyte typing III, Oxford University Press, Oxford, (1987)
CB19 is specific for the antigen CD19. This antigen has a MW of 120 kDa and contains a 280 residue extracellular domain and a 240 residue cytoplasmic domain. It is a critical signal transduction molecule that regulates B-lymphocyte development, activation, and differentiation. It plays a dominant role in establishing signalling thresholds for antigen receptors and other surface receptors on B-lymphocytes. This antigen is lost upon terminal differentiation to plasma cells.
RIV12 reacts with CD1b, a 44KDa type 1 glycoprotein associated with beta2-microglobulin. It is expressed on dendritic cells, Langerhans cells, thymocytes and T acute lymphoblastic leukemia cells. CD1, type 1 membrane protein, has structural similarity to the MHC class 1 molecule and has been shown to present lipid antigens for recognition by T lymphocytes. CD1b is also expressed in Langerhans interdigitating cells.
MutS Homolog 6 (MSH6) is a protein involved in the mismatch repair pathway. This protein is commonly associated with hereditary non-polyposis colorectal cancer, and mutations in this gene are correlated with the development of sporadic colorectal carcinoma. Studies have shown that mutations in MSH6, when co-indicated with mutations in MSH1 and MSH2, contribute to the development of sporadic colorectal carcinoma. Use of Anti-MSH2 is optimized when paired with MSH6, MLH1, and PMS2 in an IHC panel.
Excision Repair Cross Complementing 1 (ERCC1) is a DNA repair enzyme involved in the repair of UV-induced DNA damage. ERCC1 overexpression is associated with tumour progression in many malignancies, such as ovarian cancer, head squamous cell carcinoma, non-small cell lung cancer (NSCLC), and esophageal cancer.
Epithelial Cell Adhesion Molecule (EpCAM) is a transmembrane glycoprotein that mediates cell-cell adhesion in epithelia. It is normally present on most baso-lateral surfaces of normal epithelial cells and is absent in myoepithelial cells, hepatocytes, adult squamous epithelia, mesothelial cells, and fibroblasts. Anti-EpCAM stains most adenocarcinomas and neuroendocrine tumours, including small cell carcinomas. A minority of renal clear cell carcinoma, renal oncocytoma, and hepatocellular carcinoma stain positively for EpCAM, while Anti-EpCAM stains nearly all basal cell carcinoma. Anti-EpCAM stains chromophobe renal cell carcinoma, papillary renal cell carcinoma, and cholangiocarcinoma more frequently. Anti-EpCAM can be useful for distinguishing malignancy in the peritoneal and pleural cavities.
The PD-L1-IMF [IHC461] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissue within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Applications:
IHC
Clone number:
IHC461
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Tonsil, Lung Adenocarcinoma
Buffer:
Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
SPNS2 is a sphingolipid transporter expressed in the extraembryonic yolk syncytial layer (YSL). SPNS2 is required of migration of myocardial precursors, since it contributes in sphingosine 1-phosphate (S1P) secretion, which is a mediator that plays critical roles in cardiovascular, immunological, and neural development and function.
Epithelial Membrane Antigen (EMA) is a mucin glycoprotein expressed on apical epithelial cells. Anti-EMA positively stains normal and neoplastic cells including sweat glands, mammary epithelia, and squamous epithelia. Adrenal carcinoma, seminomas, paraganglioma, hepatocellular carcinoma, and embryonal carcinomas exhibit a negative stain. As Anti-EMA commonly reacts positively with meningioma, it is useful for differentiating this tumour from other intracranial neoplasms such as schwannomas.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Applications:
IHC
Clone number:
IHC085
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Breast, Skin
Buffer:
Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
The PD-L1-TEC [IHC451] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissue within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Applications:
IHC
Clone number:
IHC451
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Tonsil, Lung Adenocarcinoma
Buffer:
Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
Programmed Death-Ligand 1 (PD-L1), CD274, or B7 Homolog 1 (B7-H1), is a transmembrane protein involved in suppressing the immune system and rendering tumour cells resistant to lysis through binding of the Programmed Death-1 (PD-1) receptor. Overexpression of PD-L1 may allow cancer cells to evade the actions of the host immune system. In renal cell carcinoma, upregulation of PD-L1 has been linked to increased tumour aggressiveness and risk of death. When considered in adjunct with CD8+ tumour-infiltrating lymphocyte density, expression levels of PD-L1 may be a useful predictor of multiple cancer types, including stage III non-small-cell lung cancer, hormone receptor negative breast cancer, and sentinel lymph node melanoma.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC411
Antibody Isotype:
IgG
GMDN Code:
62046
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Tonsil, Lung Adenocarcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Galectin-3 is a lectin involved in cell adhesion, macrophage activation, angiogenesis, metastasis, and apoptosis. Anti-Galectin-3 is useful for distinguishing between benign and malignant thyroid neoplasms. Galectin-3 is also useful for recognizing anaplastic large cell lymphoma.
Programmed Death-Ligand 1 (PD-L1), CD274, or B7 Homolog 1 (B7-H1), is a transmembrane protein involved in suppressing the immune system and rendering tumour cells resistant to lysis through binding of the Programmed Death-1 (PD-1) receptor. Overexpression of PD-L1 may allow cancer cells to evade the actions of the host immune system. In renal cell carcinoma, upregulation of PD-L1 has been linked to increased tumour aggressiveness and risk of death. When considered in adjunct with CD8+ tumour-infiltrating lymphocyte density, expression levels of PD-L1 may be a useful predictor of multiple cancer types, including stage III non-small-cell lung cancer, hormone receptor negative breast cancer, and sentinel lymph node melanoma.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC441
Antibody Isotype:
IgG1, kappa
GMDN Code:
62046
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Tonsil, Lung Adenocarcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
The HBcAg [IHC205] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of chronic HBV infection tissue within the context of antibody panels, the patients clinical history and other diagnositc tests as evaluated by a qualified pathologist.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Applications:
IHC
Clone number:
IHC205
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Liver infected with Hepatitis B virus
Buffer:
Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
Myogenin belongs to a family of myogenic transcription factors, including MyoD, Myf5, and MRF4, which are critical in muscle development. Myogenin is found strictly in cells of skeletal muscle origin, and is therefore used as a biomarker for tumours of the muscle lineage, including alveolar rhabdomyosarcomas. Anti-Myogenin staining may occur in Wilms' tumour, and it labels the nuclei of myoblasts in developing muscle tissue. It is also expressed in some leiomyosarcomas.
Ki-67 is a nuclear, non-histone protein that is expressed only during active phases of the cell cycle (G1, S, G2 and M), but not in the resting phases (G0 and G1 early phase). Although the antigen has also been associated with ribosomal RNA transcription, it is strongly linked to cell proliferation and has thus been indicated as an effective marker in grading the proliferation rate of tumours, including those of the brain, breast, cervix, and prostate.
Perforin, a pore-forming protein found in the granules of cytotoxic T-lymphocytes and natural killer cells, functions to enable granzymes to enter the target cells and activate apoptosis. Perforin expression is upregulated in activated CD8+ T-cells, and these cells have been identified to have a major influence in Th1-associated inflammatory skin diseases. It has been suggested that perforin plays a role in alloimmunity, being involved in both the cytolytic process of rejection as well as downregulation of the T-cell mediated responses associated with the alloimmune response. Perforin-mediated cytotoxicity has also been linked to a number of autoimmune diseases.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC646
Antibody Isotype:
IgG1
GMDN Code:
63737
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Spleen
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Hepatitis B surface antigen (HBsAg) contains the large (L), middle (M), and small (S) surface proteins of the Hepatitis-B-Virus (HBV). It is the surface antigen of HBV, indicating current Hepatitis B infection. The body produces antibodies to HBsAg as part of the normal immune response to infection. Immunohistochemical staining for HBsAg in liver tissue is useful for the detection of HBV.
The PLAP [IHC088] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissues within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Applications:
IHC
Clone number:
IHC088
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Placenta
Buffer:
Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
Glypican-3 (GPC3) is a GPI-anchored proteoglycan involved in cell division and growth regulation. Glypican-3 is a useful tumour marker, and its expression has been shown to be upregulated in hepatocellular carcinoma (HCC), hepatoblastoma, melanoma, testicular germ cell tumours, and Wilms' tumour. Patients with HCC have presented elevated levels of GPC3 in the neoplastic liver tissues and serum, levels which are higher than detected in cirrhotic liver or liver with focal lesions, including those with hepatic adenoma and dysplastic nodules. Glypican-3 is also overexpressed in testicular germ cell tumours of certain subtypes, such as yolk sac tumours and choriocarcinoma, and in embryonal tumours.
Smooth Muscle-Myosin is a major component of smooth muscle contractile apparatus. IHC detection of Myosin, Smooth Muscle visulizes the myoepithelial cell present in both normal and in situ malignant breast and bronchioloalveolar lesions and serves a valuable biomarker to distinguish between benign and malignant tumors.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC091
GMDN Code:
57591
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Breast, Colon
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Postmeiotic Segregation Increased 2 (PMS2) is a DNA repair protein involved in mismatch repair. Mutations and deficiencies in the PMS2 gene have been linked to microsatellite instability and malignancies such as hereditary nonpolyposis colorectal cancer and endometrial cancer. Expression levels of the PMS2 protein may be useful as a screening tool for Lynch syndrome after a colorectal cancer diagnosis. Anti-PMS2 is recommended to be used as part of a panel along with antibodies against MSH2, MSH6, and MLH1.
Podoplanin is a transmembrane mucoprotein specifically expressed in the endothelium of lymphatic capillaries, while remaining absent from the blood vasculature. The protein is co-localized with VEGFR3/FLT4 in normal skin and kidney. Anti-Podoplanin is useful in the identification of lymphangiomas, Kaposi's sarcomas, epithelioid mesotheliomas, hemangioblastomas, seminomas, and some angiosarcomas that likely have lymphatic differentiation.
Nanog is a homeoprotein that functions with pluripotent factors, such as Oct-4 and SOX2, to maintain embryonic stem cell pluripotency. Expression of this protein has been noted in seminoma, dysgerminoma, embryonal carcinoma, and other undifferentiated germ cell tumours, while Nanog expression is absent in normal adult organ tissues. Anti-Nanog may be useful in distinguishing between undifferentiated germ cell tumours and non-germ cell tumours.
The PRAME [IHC092] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissues within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC092
GMDN Code:
65250
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Melanoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Epidermal Growth Factor Receptor (EGFR) is a tyrosine kinase present in gliocytes, epithelial cells, fibroblasts, keratinocytes, and other cell types. EGFR is overexpressed in various cancers including those of the colon, pancreas, oropharynx, stomach, and non–small cell lung, as well as head and neck squamous carcinoma and anal squamous carcinoma. EGFR expression is common in breast cancer, especially in triple-negative and basal-like breast carcinomas, and recent research has also found EGFR expressed in malignant bone and soft tissue cancers. Anti-EGFR is useful for detecting epithelioid and synovial sarcoma.
Human Chorionic Gonadotropin (hCG) is a glycoprotein hormone produced by the trophoblastic cells of the placenta after conception. Anti-hCG is useful for identifying trophoblastic tumours, such as choriocarcinoma. hCG is also a marker for non-trophoblastic tumours such as large cell carcinoma and lung adenocarcinoma.
GATA3 is a transcription factor important in cell proliferation, development, and differentiation. GATA3 is mostly observed in breast and urothelial carcinomas, and is rarely present in other cancers such as endometrial endometrioid adenocarcinoma. Among the breast carcinomas, GATA3 has a lower expression in luminal B subtype breast carcinoma. Studies have found GATA3 expression to be associated with ER (estrogen receptor), PR (progesterone receptor), and HER2 in breast cancer cases. Urothelial carcinomas stain positively for GATA3 in invasive or high grade tumours, therefore Anti-GATA3 is useful for carcinoma diagnosis when those of the breast and bladder are plausible.
Napsin A is a pepsin-like aspartic proteinase that is closely related to Napsin B. It is expressed mainly in the lung and kidney, and is involved in the correct folding, targeting, and control of aspartic proteinase zymogens. Napsin A expression has been indicated in type II pneumocytes and adenocarcinomas of the lung and kidney. Anti-Napsin A is also useful for differentiating between primary lung adenocarcinomas and adenocarcinomas of other organs, due to the high expression of Napsin A in adenocarcinomas of the lung.
LAG3 (Lymphocyte-activation gene 3) was discovered in 1990 and was previously designated as CD223. LAG3 is a cell surface molecule expressed by activated T cells, natural killer cells, B cells and plasmacytoiddendritic cells. It binds to major histocompatibility complex (MHC) class II molecules and serves as an immune checkpoint receptor. LAG3 negatively regulates cellular proliferation, activation and homeostasisof T cells, and plays a role in Treg suppressive function. LAG3 also helps maintain CD8+ T cells in atolerogenic state and, working with PD-1, helps maintain CD8 exhaustion during chronic viral infection. LAG3 expression was detected in tumor infiltrating lymphocytes. IHC revealed LAG3 expression was distributed on lymphocytes scattered in renal cell carcinoma, melanoma and lymphomas. They were also detected in the tumor stroma as well as in the peritumoral tissue. LAG3 is the target of various drug development programs for cancer and autoimmune disorders. In soluble form, it is also being developed as a cancer drug in its own right.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC127
GMDN Code:
?
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Tonsil
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five types) and two light chains - kappa and lambda. The determination of light chain ratio is critical in evaluating B-cell neoplasms, as the majority of B-cell lymphomas express either kappa or lambda light chains, while a mixture of kappa and lambda is characteristic of reactive proliferations. Antibodies against lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas.
N-cadherin, also known as Cadherin-2 (CDH2) or Neural Cadherin (NCAD), is a transmembrane cell adhesion molecule that was originally detected in nervous tissue. It plays an important role in embryogenesis, being involved in gastrulation and neural crest development. N-cadherin is found in cancer cells and allows for transendothelial migration, which is a critical process in the metastasis of cancer. Overexpression and disorderly arrangement of N-cadherin has been noted in dilated cardiomyopathy. It has been suggested that, when considered in adjunct with the status of a number of additional cell-cell adhesion molecules, missense mutations in N-cadherin may be a potential indicator of obsessive-compulsive disorder and Tourette disorder.
LMO2, also known as LIM-Only Transcription Factor 2, RBTN2, or TTG2, is an oncoprotein that is expressed in normal germinal center B-cells, as well as bone marrow hematopoietic precursors and endothelial cells. LMO2 plays a role in angiogenesis and hematopoesis, and its expression has been detected in erythroid and myeloid precursors, megakaryocytes, and also in lymphoblastic and acute myeloid leukemias. LMO2 protein expression has been noted in diffuse large B-cell lymphoma, the most common adult non-Hodgkin's lymphoma, as well as follicular lymphoma, a neoplasm derived from germinal center B-cells that accounts for a number of cases of non-Hodgkin's lymphomas.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC615
Antibody Isotype:
IgG1, kappa
GMDN Code:
63898
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Tonsil, Follicular Lymphoma, Diffuse Large B-Cell Lymphoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Nerve Growth Factor Receptor (NGFR), also known as p75, P-75NTR, or CD271, is a neurotrophin receptor belonging to the tumour necrosis factor receptor family. It is expressed mainly in Schwann cells and neurons, as well as a number of other non-neuronal cell types, and is also expressed in melanocytes, melanomas, neuroblastomas, pheochromocytomas, neurofibromas, neurotized nevi (type C melanocytes), and other neural crest cell or tumour derivatives. It has been suggested that NGFR may act as a tumour suppressor indicated in prostate and urothelial cancer, and Anti-NGFR is often used in adjunct with S100, to aid in the diagnosis of desmoplastic and neurotrophic malignant melanomas. Anti-NGFR is also useful as an aid in the diagnosis of breast malignancy, as the antibody labels the myoepithelial cells of breast ducts and intralobular fibroblasts of breast ducts.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC637
Antibody Isotype:
IgG1, kappa
GMDN Code:
57471
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Breast
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
The Lp-PLA2 [IHC407] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of cardiovascular diseases within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Applications:
IHC
Clone number:
IHC407
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Tonsil, Thymus, Placenta
Buffer:
Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
Fibronectin is a glycoprotein that contributes to cell adhesion, migration, and metastasis. Renal cancer cells exhibit higher expression of fibronectin, therefore Anti-Fibronectin is useful for assessing the progression and aggressiveness of renal cancer cells.
The Nestin [IHC105] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissues within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Applications:
IHC
Clone number:
IHC105
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Tonsil, Glioma
Buffer:
Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
Luteinizing Hormone (LH) is a reproductive hormone produced and secreted by the gonadotropes in the anterior pituitary gland. LH functions to stimulate ovulation in females and the production of testosterone from the Leydig cells in males. This hormone is useful for the study of pituitary disease, and acts as a clinical marker that is useful for classifying tumours of the pituitary.
Neurofilaments are a group of intermediate filaments found abundantly around the axons of vertebrate neurons. They are also expressed in paragangliomas, adrenal pheochromocytomas, Merkel cell tumours, carcinoid tumours, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung. Anti-Neurofilament stains a variety of neural, neuroendocrine, and endocrine tumours, such as neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas.
Glutamine Synthetase (GS-6 or GS) catalyzes the conversion of glutamate and ammonia to glutamine in the liver, and is expressed in pericentral hepatocytes, but not in periportal hepatocytes or in the mid-zonal. Anti-Glutamine Synthetase is useful in some hepatocellular carcinomas and many high grade dysplastic nodules, and therefore may be useful in recognizing these cases. A panel of antibodies against HSP70 (heat shock protein 70), GPC3, and glutamine synthetase is useful for differentiating dysplastic from early malignant hepatocellular nodules in cirrhosis. GS staining of hepatocellular lesions is useful for the differential diagnosis of focal nodular hyperplasia (FNH), hepatic adenoma (HCA), dysplastic nodules, and low grade hepatocellular carcinoma. FNH produces a “map-like” pattern when stained with Anti-Glutamine Synthetase. Conversely, HCA can stain negatively, produce border staining, or stain around the tumour veins.
Neuron-Specific Enolase (NSE), also known as Enolase 2 (ENO2), is one of three enolase enzymes found in mammals, and acts as a phosphopyruvate hydratase. This mammalian glycolytic isoenzyme is located specifically in neurons of neuroendocrine cells, as well as tumours associated with those neurons. However, it has also been detected immunohistochemically in non-neoplastic cells of the pituitary, peptide-secreting tissues, pinealocytes, neuroendocrine cells of the lung, thyroid, parafollicular cells, adrenal medulla, islets of Langerhans, Merkel cells of the skin, and melanocytes. NSE is a useful marker for identifying normal striated muscle, hepatocytes, and peripheral nerves. Anti-NSE may detect for neuroendocrine differentiation, only when used in a panel of antibodies including more specific markers such as synaptophysin, chromogranin, and neurofilament.
Anti-Kappa recognizes surface immunoglobulin on normal and neoplastic B-cells, and has been indicated as a potential aid in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas, where the expression of a single light chain class is restricted. The determination of light chain ratio is critical in evaluating B-cell neoplasms, as the majority of B-cell lymphomas express either kappa or lambda light chains, while a mixture of kappa and lambda is characteristic of reactive proliferations. In paraffin-embedded tissue, Anti-Kappa displays strong staining of kappa-positive plasma cells, as well as cells that have absorbed exogenous immunoglobulins.
Estrogen Receptors (ER) are a group of nuclear hormone receptors activated by the hormone estrogen. ER is found in normal epithelial cells of the breast and endometrium, as well as in breast cancer cells.
Octamer-Binding Transcription Factor 4 (Oct-4), also known as POU5F1 (POU Domain, Class 5, Transcription Factor 1), is a member of the POU homeodomain family of transcription factors and is involved in the maintenance and regulation of pluripotency in embryonic stem and germ cells. Anti-Oct-4 is highly useful and sensitive for seminomas, germinoma, dysgerminoma, embryonal carcinoma, and gonadoblastoma. Oct-4 may be associated with tumourigenesis, and can have an effect on some aspects of tumour behavior, including tumour recurrence or resistance to therapies.
Glial Fibrillary Acidic Protein (GFAP) is an intermediate filament protein that is present in astrocytes and some ependymal cells of the central nervous system. In the peripheral nervous system, GFAP is present in Schwann cells, enteric glial cells, and satellite cells. Anti-GFAP staining is useful in differentiating neoplasms of astrocyte origin from other neoplasms in the central nervous system.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC584
Antibody Isotype:
IgG
GMDN Code:
57238
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Brain
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
OX40 is a member of the tumor necrosis factor (TNF) receptor superfamily, which regulates T cell activity and immune response. OX40 is mainly activated by CD4 + and CD8 + T cells. It is expressed on cells, while OX40 ligands (OX40L, TNFSF4, CD252) are mainly expressed on activated antigen-presenting cells. Research shows that OX40 pathway is related to inflammation and autoimmune diseases. Other studies have shown that OX40 agonists can enhance the anti-tumor immunity of several cancer types.
MART-1, also known as Melan A or Melanoma Antigen Recognized by T-Cells 1, is a protein antigen found specifically on melanocytes of normal skin, retina, and nevi, and not in other normal tissues. Anti-MART-1 is therefore useful as a marker for melanocytic tumours, and as an aid in establishing the diagnosis of metastatic melanomas.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC408
Antibody Isotype:
IgG1
GMDN Code:
57393
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Melanoma, Skin
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Anti-Kappa recognizes surface immunoglobulin on normal and neoplastic B-cells, and has been indicated as a potential aid in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas, where the expression of a single light chain class is restricted. The determination of light chain ratio is critical in evaluating B-cell neoplasms, as the majority of B-cell lymphomas express either kappa or lambda light chains, while a mixture of kappa and lambda is characteristic of reactive proliferations. In paraffin-embedded tissue, Anti-Kappa displays strong staining of kappa-positive plasma cells, as well as cells that have absorbed exogenous immunoglobulins.
Flt-1, also known as Fms Related Tyrosine Kinase 1 or VEGFR1 (Vascular Endothelial Growth Factor Receptor 1), is a tyrosine kinase involved in lymphangiogenesis, angiogenesis, and wound healing. It is present in endothelial cells, osteoblasts, placental trophoblasts, renal mesangial cells, and some hematopoietic stem cells. Anti-Flt-1/VEGFR1 is useful for identifying carcinomas of the larynx and esophagus.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC086
GMDN Code:
?
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Angiosarcoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Follicle-Stimulating Hormone (FSH) allows for progression of ovarian folliculogenesis, and enables Sertoli cell proliferation in the testis. Anti-FSH reacts with FSH-producing cells, and therefore FSH staining is useful for classifying pituitary cancers and understanding pituitary disease.
MART-1, also known as Melan A or Melanoma Antigen Recognized by T-Cells 1, is a protein antigen found specifically on melanocytes of normal skin, retina, and nevi, and not in other normal tissues. Anti-MART-1 is therefore useful as a marker for melanocytic tumours, and as an aid in establishing the diagnosis of metastatic melanomas.
The MCT4 [IHC124] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissues within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Applications:
IHC
Clone number:
IHC124
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Cervical Cancer
Buffer:
Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
p120 Catenin is a nucleolar protein belonging to the armadillo protein family, which is involved in cell-cell adhesion and signal transduction. p120 Catenin is associated with proliferation, and is found in the majority of human malignant tumours, while remaining absent from resting normal cells. Anti-p120 Catenin is useful in differentiating between ductal and lobular neoplasia in the breast, and strong staining with Anti-p120 Catenin is associated with discohesive infiltrative morphology in gastric and colonic carcinoma. Accumulation of p120 Catenin in the cytoplasm has been linked to lung cancer, pancreatic cancer, and gastric cancer, and is correlated to poor prognosis in colon cancer.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC120
Antibody Isotype:
IgG1
GMDN Code:
62843
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Lobular Breast Carcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
The Factor XIIIa [IHC572] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissue within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Applications:
IHC
Clone number:
IHC572
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Placenta
Buffer:
Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
Mouse Double Minute 2 Homolog (MDM2), also known as HDM2 in humans, is crucial in negative regulation of the p53 tumour suppressor. Negative regulation is mediated through both the ubiquitination of p53/TP53, as well as inhibition of p53 transcriptional activation. Reports have indicated an overexpression of MDM2 to be associated with a number of different human tumour types, including soft tissue sarcomas, osteosarcomas, and breast tumours. When co-overexpressed with the CDK4 protein, MDM2 can also aid in the detection of well-differentiated liposarcomas and de-differentiated liposarcoma.
The p16 (p16<sup>INK4A</sup>) protein is a cyclin-dependent kinase (CDK) inhibitor that plays an important regulatory role in the cell cycle. By controlling the transition between the G1 and S phases through regulation of retinoblastoma protein, p16 decelerates cellular differentiation and therefore acts as a tumor suppressor, making it the key marker in several human cancers including head and neck cancer, perianal lesions, melanomas, gliomas, lymphomas, and some types of leukemia. p16 is also clinically indicated in carcinomas of the esophagus, pancreas, lung, biliary tract, liver, colon, and urinary bladder.
The p16 (p16<sup>INK4A</sup>) protein is a cyclin-dependent kinase (CDK) inhibitor that plays an important regulatory role in the cell cycle. By controlling the transition between the G1 and S phases through regulation of retinoblastoma protein, p16 decelerates cellular differentiation and therefore acts as a tumor suppressor, making it the key marker in several human cancers including head and neck cancer, perianal lesions, melanomas, gliomas, lymphomas, and some types of leukemia. p16 is also clinically indicated in carcinomas of the esophagus, pancreas, lung, biliary tract, liver, colon, and urinary bladder.
p21, also known as p21^ Cip1 ^ , p21^ Waf1 ^, Cyclin-Dependent Kinase Inhibitor 1, or CDK-Interacting Protein 1, functions to regulate cell cycle progression at G1 by inhibiting the activity of Cyclin-CDK2 or -CDK4 complexes. This cyclin-dependent kinase inhibitor is expressed in all adult human tissues, and decreased expression of p21 is linked to poor prognosis in a number of carcinomas including gastric carcinoma, non-small cell lung carcinoma, and thyroid carcinoma. p21 is also associated with favourable prognosis in several tumours.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC021
GMDN Code:
57493
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Tonsil
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
p27, also known as p27<sup>Kip1</sup>, is a cyclin-dependent kinase inhibitor that binds to and inhibits cyclin-dependent kinases, thereby regulating progression from G1 to S phase. Decreased expression of p27 is linked to poor prognosis in renal-cell carcinoma, colon carcinoma, small breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, lymph node metastases in papillary carcinoma of the thyroid, and is associated with a more aggressive phenotype of carcinoma in the cervix.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC128
GMDN Code:
57500
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Tonsil
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
MutL Homolog 1 (MLH1) is a protein involved in the mismatch-repair pathway. This protein is commonly associated with hereditary non-polyposis colorectal cancer, as the MLH1 gene is frequently mutated in patients with this cancer. Studies have shown MLH1 to be deficient in a high percentage of patients with microsatellite instability, as well as endometrial and ovarian cancers. Use of Anti-MLH1 is optimized when paired in an IHC panel with MSH6, MSH2, and PMS2. Anti-MLH1 is useful in the detection of MLH1 in a number of normal and neoplastic tissues, and for identifying a loss of MLH1 in tumours that are microsatellite-unstable.
Anti-p40 recognizes squamous and basal cells, the shortest variant of p53, and ΔNp63 (an isoform of p63). p40 has been indicated as an alternative to p63 for the detection of Squamous Cell Carcinoma (SqCC), offering the advantage of eliminating potential misinterpretation of a positive adenocarcinoma as a SqCC.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC058
Antibody Isotype:
IgG1
GMDN Code:
64957
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Squamous Cell Carcinoma of Lung
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
p504s, also known as ?-Methylacyl Coenzyme A Racemase (AMACR), is an enzyme localized in the peroxisome and mitochondria that functions in ?-oxidation of branched chain fatty acids, as well as bile synthesis. AMACR has been clinically indicated as a tissue biomarker for prostate cancer and colorectal cancer, as well as high-grade prostatic intraepithelial neoplasia, a precursor lesion of prostate cancer. p504s overexpression has also been detected in a number of other cancers including ovarian, breast, bladder, lung, and renal cell carcinomas, lymphoma, and melanoma.
E-cadherin is an intercellular adhesion molecule present in epithelial cells. Anti-E-cadherin stains glandular epithelium, as well as lung, gastrointestinal, and ovarian adenocarcinomas. A panel of antibodies against E-cadherin and p120 is also used to differentiate ductal (membranous staining) and lobular breast cancer (cytoplasmic staining). Anti-E-cadherin also stains some thyroid cancers.
DOG1, also known as Discovered on GIST-1, is a marker that is highly specific for gastrointestinal stromal tumour (GIST). Anti-DOG1 is extremely sensitive for the detection of GIST and its diagnosis. Although some GIST stain weakly for c-kit, DOG1 is expressed in the vast majority of GIST cases. Reports have also indicated DOG1 as a marker for salivary acinar and intercalated duct differentiation.
Vimentin is a component of intermediate filament in mesenchymal cells, such as endothelial cells, fibroblasts, lymphocytes, and melanocytes. Anti-Vimentin is useful for assessing whether tissue samples have been processed and preserved properly. A panel of Anti-Vimentin and Anti-Keratin is useful for differentiating melanomas from large cell lymphomas and undifferentiated carcinomas. This diagnostic grade Vimentin IVD antibody stains melanomas and schwannomas, as well as endometrial endometrioid adenocarcinomas.
Desmoglein-3 Antibody (DSG3) is a component of desmosomes in vertebrate epithelial cells. It identifies pulmonary squamous cell carcinomas from other types of lung cancer with a highly specificity and sensitivity. Studies show the upregulation of DSG3 correlated with metastasis in a number of cancers including lung cancers. The expression of DSG3 indicates a poor prognosis and portends a more aggressive clinical outcome.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC083
Antibody Isotype:
IgG1
GMDN Code:
?
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Skin Cancer
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Desmin is a type III intermediate filament present in normal smooth, skeletal, and cardiac muscle cells. Analysis by light microscopy suggests desmin localizes towards the periphery of Z-lines in striated muscle fibrils. Desmin connects cytoplasmic dense bodies to membranous dense plaques in smooth muscles. Anti-Desmin stains rhabdomyomas, leiomyosarcoma, rhabdomyosarcoma, leiomyomas, and perivascular cells from skin glomus tumours, and is used to identify the myogenic characteristics of tumours. Desmin can also be found in myofibroblasts and desmoid fibromatosis.
The D-Dimer [IHC085] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissues within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Applications:
IHC
Clone number:
IHC085
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Glioma
Buffer:
Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
Cytokeratin 8 & 18 are present in various epithelia including that of the breast, thyroid, respiratory tract, and gastrointestinal tract. Anti-Cytokeratin 8 & 18 stains adenocarcinomas and most non-keratinizing squamous carcinomas, but does not stain keratinizing squamous carcinomas. Since Cytokeratin 18 is scarce in normal epidermis, Anti-Cytokeratin 8 & 18 is used to detect Paget cells in such samples. Cytokeratin 8 & 18 helps identify colorectal carcinoma metastases as it is more sensitive than genetic tests.
Cytokeratin 8 (CK8) is present in single-layer epithelial tissue. CK8 frequently interacts with Cytokeratin 18, and Anti-Cytokeratin 8 is useful for identifying adenocarcinomas with simple epithelium origin. It may also be used to differentiate between lobular (perinuclear staining) and ductal (peripheral staining) breast carcinomas.
Villin is a tissue-specific actin-binding glycoprotein that is associated with the maintenance of the microvilli brush border found in the gastrointestinal mucosal epithelium. Villin is expressed in carcinomas of the gastrointestinal tract, renal cell carcinomas, pacreatic carcinomas, endometrial carcinomas, as well as carcinomas of the ovary and lungs. Anti-Villin antibodies can be useful for identifying and differentiating adenocarcinomas of these organs from other organs in the body. Additionally, it may be helpful in separating carcinoid tumors from other endocrine tumors.
Vascular Endothelial Growth Factor (VEGF) promotes vasculogenesis and angiogenesis, and mainly affects the vascular endothelium. VEGF is associated with poor prognoses of breast carcinomas, and has been shown to be elevated in rheumatoid arthritis.
Thyroid Transcription Factor 1 (TTF-1) is present in diencephalon, lung, and thyroid. Anti-TTF-1 stains thyroid and thyroid-derived tumours, and is therefore used for distinguishing lung adenocarcinoma from germ cell tumours, malignant mesothelioma, and metastatic carcinomas from organs other than the thyroid. It is also useful for distinguishing small cell lung carcinoma from lymphoid infiltrates, and pulmonary from non-pulmonary adenocarcinomas in malignant effusions. The ability to distinguish between pulmonary and non-pulmonary adenocarcinomas is particularly useful in identifying tumours that have metastasized to the brain.
Thyroid-Stimulating Hormone (TSH) is secreted by thyrotrope cells in the anterior pituitary gland. Anti-TSH stains thyrotrophs and is useful for categorizing pituitary tumours, as well as for recognizing primary and metastatic pituitary gland tumours.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC679
Antibody Isotype:
IgG1
GMDN Code:
57663
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Pituitary
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
STAT6 is a member of the signal transducers and activators of transcription (STAT) family. Recurrent fusions of STAT6 with NAB2 on chromosome 12q13 have been found in the majority of solitary fibrous tumors (SFT). STAT6 antibody is a reliable immunohistochemical marker for SFT and can be helpful to distinguish this tumor type from histologic mimics.
Stathmin regulates microtubule dynamics in the cell cycle. It is present in all tissues, but is mostly pronounced in constantly proliferating cell types. Anti-Stathmin staining has been found to correlate with cervical intraepithelial neoplasia (CIN) grade, with CIN 3 presenting the greatest expression and CIN 1 displaying the least expression of stathmin.
The TOP2A [IHC112] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of proliferating cells in normal and neoplastic tissues within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Applications:
IHC
Clone number:
IHC112
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Tonsil, Cervix
Buffer:
Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
p53, also known as Tumour Protein 53 or TP53, is a tumour suppressor and transcription factor that functions in a number of anti-cancer activities including DNA repair, cell-cycle arrest, and apoptosis in response to DNA damage or other stressors. Mutations in p53 are linked to a number of malignant tumours, including those of the breast, ovary, bladder, colon, lung, and melanoma. Anti-p53 staining has been used to detect intratubular germ cell neoplasia, and also to distinguish between uterine serous carcinoma and endometrioid carcinoma.
Survivin is an apoptosis inhibitor that is nearly undetectable in terminally differentiated cells, but found in most tumours including renal cell carcinoma, ovarian carcinoma, hepatocellular carcinoma, prostate carcinoma, and breast carcinoma. Survivin expression is linked to tumour progression, but not patient survival.
Synaptophysin is a synaptic vesicle glycoprotein used in synaptic transmission of neurons. Anti-Synaptophysin stains the gastrointestinal mucosa and lung neuroendocrine cells of the human adrenal medulla, carotid body, pancreas, pituitary, skin, and thyroid. Synaptophysin also stains neuroendocrine neoplasms. Use of Anti-Synaptophysin produces diffuse, finely granular, cytoplasmic staining. The presence of synaptophysin does not correlate with neuron-specific enolase or other neuroendocrine markers.
MutS Homolog 2 (MSH2) is a protein involved in the mismatch-repair pathway. This protein is commonly associated with hereditary non-polyposis colorectal cancer, and mutations in this gene are correlated with the development of sporadic colorectal carcinoma. Expression levels of MSH2 are abnormally low in a high percentage of patients with microsatellite instability, as well as endometrial and ovarian cancers. Use of Anti-MSH2 is optimized when paired in an IHC panel with antibodies against MSH6, MLH1, and PMS2. Reports have shown Anti-MSH2 to be useful in the detection of the protein in a number of normal and neoplastic tissues, and for identifying a loss of MSH2 in tumours that are microsatellite-unstable.
Tumour-Associated Glycoprotein 72 (TAG-72) is a glycoprotein found on the surface of many cancer pathologies. Anti-TAG-72 can be useful for detecting some adenocarcinomas and non-neoplastic tissues. This diagnostic grade TAG-72 IVD antibody is useful for identifying adenocarcinomas in positive staining, but in mesotheliomas no staining is observed.
Topoisomerase II alpha (TOP2A) is a member of a highly conserved group of enzymes that plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. The overexpression of TOP2A has been correlated with increased risk of progression in various cancers, and it has been a target for the development of anti-polymerase inhibitors to treat cancer.
Glycophorin A (GPA) and Glycophorin B (GPB) are erythrocyte blood group determinants that minimize erythrocyte aggregation during the circulation of blood. Anti-Glycophorin A is useful for understanding erythroid cell development and identifying erythroid leukemias.
Erythroblastosis Virus E26 Transforming Sequence Related Gene (ERG) facilitates endothelial homeostasis. ERG is found in malignant and benign vascular endothelial tumours, including hemangiomas and Kaposi's sarcoma. ERG is present in various prostate carcinomas, but is absent in breast, colon, and urothelium carcinomas. Anti-ERG is useful for differentiating prostate carcinoma from non-prostatic epithelial tumours, and for recognizing vascular endothelial neoplasms.
p57<sup>Kip2</sup>, also known as p57, is a tumour suppressor protein that causes cell cycle arrest at G1 by binding to G1 cyclin-CDK complexes. The p57<sup>Kip2</sup> gene is a potential tumour suppressor target as the gene is located in a chromosomal region implicated in sporadic cancers, Wilms' tumour, and Beckwith Wiedemann syndrome. Anti-p57<sup>Kip2</sup> labels many cytotrophoblast nuclei and stromal cells in normal placenta, and is useful in differentiating between complete hydatidiform mole and partial hydatidiform mole or hydropic abortion.
Enhancer of Zeste Homolog 2 (EZH2) is a methylase of histone H3 that silences gene expression in those regions. EZH2 is overexpressed or mutated in gastric, prostate, uterine, breast, and renal cell cancers, as well as in melanoma and most B- and T-cell lymphomas. Although EZH2 is usually present in follicular centers, it is not expressed in the mantle zones, plasma cells, follicular or interfollicular T-lymphocytes, natural killer T-lymphocytes, plasmacytoma, lymphoplasmacytic lymphoma, or MALT lymphoma. EZH2 is rarely present in normal breast duct epithelium and in normal and hyperplastic lymph node. Anti-EZH2 is also useful for detecting lymphoma and non-small cell lung cancers. EZH2 is associated with tumour proliferation and can be used in staining panels to distinguish aggressive lymphomas from less aggressive lymphomas or normal cells.
Mucin 1 (MUC1) is a membrane-bound glycoprotein involved in a number of protective and cell-signaling functions, including cell-cell adhesion, proliferation, motility, invasion, and survival. Overexpression of MUC1 is clinically indicated in breast carcinomas, papillary thyroid carcinomas, and thymic carcinomas, and reports have named MUC1 as a useful marker for differentiating thymic carcinoma from type B3 thymoma. The expression of MUC1 is correlated with the grade of malignancy in thymic epithelial tumours, and loss of MUC1 expression has been associated with reactive gastropathy. MUC1 is not expressed in normal human epidermis, but it has been detected in the epidermis of psoriatic plaques of biopsies from patients diagnosed with psoriasis vulgaris.
p63 is a tumour suppressor protein that is very similar to p53 in structure and function, while being homologous to p73. p63 is important in development and differentiation, and has been identified as a useful marker for distinguishing between lung squamous cell carcinomas and adenocarcinomas. Anti-p63 is also used to differentiate between benign and malignant prostate and breast lesions, due to its labeling of the nuclei of myoepithelial cells in both tissue types.
KBA.62, also known as Melanoma Associated Antigen, is used to detect an antigen present in melanocytic tumours, such as melanomas, due to its proven sensitivity and specificity. The antibody can also be used to distinguish between junctional nevus and intradermal nevus cells, and fetal melanocytes versus normal adult melanocytes. Studies have shown KBA.62 to be highly useful in differentiating between metastatic amelanotic melanoma and a number of poorly differentiated carcinomas, large cell lymphomas, sarcomas, and spindle cell carcinomas.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC062
Antibody Isotype:
IgG1
GMDN Code:
57371
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Melanoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
The NRP1 [IHC121] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissues within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Applications:
IHC
Clone number:
IHC122
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Brain
Buffer:
Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
Parathyroid Hormone (PTH), also known as Parathormone or Parathyrin, is a hormone secreted by the parathyroid glands that functions to increase the concentration of calcium in the blood. Anti-Parathyroid Hormone (PTH) is useful for differentiating parathyroid hyperplasia/neoplasms from thyroid and metastatic neoplasms, and is also used in the consideration of parathyroid carcinomas located primarily in the anterior mediastinum.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC645
Antibody Isotype:
IgM
GMDN Code:
63169
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Parathyroid Tissue
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Mucin 5AC (MUC5AC) is a secretory-type mucin found in columnar mucous cells of surface gastric epithelium and in goblet cells of the fetal and precancerous colon, but not in normal colon cells. MUC5AC expression is indicated in carcinomas wherein the type is defined as diffuse and infiltrative, and those located mainly in the antrum. Studies have also suggested a correlation between MUC5AC and colorectal signet ring cell carcinoma, with overexpression of MUC5AC relating to the carcinogenesis, malignant potential, progression, and clinical behaviors.
PAX5 is a member of the paired box (PAX) family of transcription factors, which are key regulators in early development. The PAX5 gene encodes the B-cell lineage specific activator protein (BSAP), whose expression is limited to early stages of B-cell differentiation. Anti-PAX5 is useful in differentiating between classic Hodgkin's lymphoma versus multiple myeloma and solitary plasmacytoma, as the protein is expressed in mature and precursor B-cell non-Hodgkin's lymphomas/leukemias while being absent from the other two conditions. Diffuse large B-cell lymphomas are positive for PAX5, with the exception of those with terminal B-cell differentiation, and T-cell neoplasms do not stain with Anti-PAX5.
T-Cell Immunoglobulin and Mucin-Domain-Containing Molecule-3 (TIM3) is present on T-helper type 1 lymphocytes and other immune cells, including dendritic cells and natural killer cells. TIM3 is overexpressed in CD4+ tumour-infiltrating lymphocytes, including those with non-small cell lung cancer associated with poor prognoses. TIM3 has recently emerged as a potential target for cancer immunotherapy.
TIGIT is an immune receptor present on some T cells and Natural Killer cells. TIGIT binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. Through the CD226/TIGIT-PVR pathway, TIGIT regulates T cell mediated immunity. In cancer, TIGIT and PD-1 have been shown to be over-expressed on tumor antigen-specific CD8+ T cells and CD8+ tumor infiltrating lymphocytes (TILs) from individuals with melanoma. Blockade of TIGIT and PD-1 led to increased cell proliferation, cytokine production, and degranulation of tumor antigen-specific CD8+ T cells and TIL CD8+ T cells. It can be considered an immune checkpoint.
Mucin 6 (MUC6) is a glycoprotein expressed in mucous neck cells, pyloric glands of the antrum, epigastric and bronchial epithelium, and in Müller ducts of the endocervix and urethral epithelium. Anti-MUC6 is useful for differentiating fetal, precancerous, and cancerous colonic mucosa from normal colon, as the antibody does not stain the latter. Anti-MUC6 stains the gastric epithelial surface of normal human gastrointestinal tracts.
FOXP3 is a forkhead transcription factor family member which plays a key role in CD4+CD25+ regulatory T cell function and represents a specific marker for these cells. Specifically in IHC, FOXP3 is a marker for adult T-cell leukemia/lymphoma (ATLL). In normal lymphoid tissues, a T-cell subset in interfollicular areas shows nuclear staining. There are many characteristics of FOXP3s role in cancer, which involves tumour progression through the suppression of T-cell activity and oncogene suppression through suppressing the expression of HER2, Skp2, SATB1 and MYC oncogenes.
Terminal Deoxynucleotidyl Transferase (TdT) is a DNA polymerase present in normal and malignant pre-B- and pre-T-cells during early differentiation. Anti-TdT stains nearly all acute lymphoblastic lymphoma/leukemia (ALL) cases, but does not stain pre-B-cell ALL or mature B- and T-cells. Anti-TdT staining is also useful for identifying Type AB thymoma and some chronic myeloid leukemia.
PAX8 is expressed in simple ovarian inclusion cysts and non-ciliated mucosal cells of the fallopian tubes, but is absent from normal ovarian surface epithelial cells. Mutations in the PAX8 gene are linked to thyroid follicular carcinomas, atypical thyroid adenomas, and thyroid dysgenesis. Reports have associated PAX8 expression with renal carcinoma, nephroblastoma, and seminoma, and have indicated PAX8 as a useful marker for renal epithelial tumours, ovarian cancer, and for differential diagnoses in lung and neck tumours. Anti-PAX8 can be useful in determining the primary site of invasive micropapillary carcinomas of ovary from bladder, lung, and breast, when used in adjunct with a panel of organ-specific markers such as uroplakin, mammaglobin, and TTF-1.
PAX8 is expressed in simple ovarian inclusion cysts and non-ciliated mucosal cells of the fallopian tubes, but is absent from normal ovarian surface epithelial cells. Mutations in the PAX8 gene are linked to thyroid follicular carcinomas, atypical thyroid adenomas, and thyroid dysgenesis. Reports have associated PAX8 expression with renal carcinoma, nephroblastoma, and seminoma, and have indicated PAX8 as a useful marker for renal epithelial tumours, ovarian cancer, and for differential diagnoses in lung and neck tumours. Anti-PAX8 can be useful in determining the primary site of invasive micropapillary carcinomas of ovary from bladder, lung, and breast, when used in adjunct with a panel of organ-specific markers such as uroplakin, mammaglobin, and TTF-1.
Glucose transporter type I (GLUT1), also known as SCL2A1, is a glucose transporter present in the blood-brain barrier and erythrocytes. GLUT1 overexpression is associated with tumour progression or poor prognoses of bladder, breast, cervical, colon, and lung carcinomas, as well as mesothelioma. Anti-GLUT1 is useful for distinguishing malignant mesothelioma (GLUT1(+)) from reactive mesothelium (GLUT1(-)).
Multiple Myeloma Oncogene-1 (MUM1), also known as Interferon Regulatory Factor 4 (IRF4), is a transcription factor present in a variety of hematolymphoid neoplasms and in malignant melanoma, but is absent from other human tumours. MUM1 expression has been indicated in both pediatric and adult diffuse large B-cell lymphoma (DLBCL), and, when the immunostaining status of CD10 and Bcl6 is also considered, Anti-MUM1 can be used to sub-distinguish germinal center type DLBCL from the non-germinal center type. Anti-MUM1 stains normal melanocytes, melanocytic nevi, and malignant melanoma in non-hematopoietic tissues, and can also stain other B-cell lymphomas such as lymphoplasmacytic lymphoma, grade 3 follicular lymphoma, primary central nervous system lymphoma, primary mediastinal large B-cell lymphoma, Burkitt-like lymphoma, and classic Hodgkin's lymphoma.
The PCNA [IHC711] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissues within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
Transcription Factor E3 (TFE3) is a transcription factor that binds to the MUE3-type E-box sequences involved in TGF-β signaling. Anti-TFE3 staining is the most sensitive and specific indicator of Xp11 translocation renal cell carcinomas. Since alveolar soft part sarcoma (ASPS) is characterized by a specific chromosomal rearrangement resulting in a chimeric transcription factor (ASPSCR1-TFE3), this TFE3 IVD antibody is also a useful diagnostic tool for recognizing ASPS.
Ki-67 is a nuclear, non-histone protein that is expressed only during active phases of the cell cycle (G1, S, G2 and M), but not in the resting phases (G0 and G1 early phase). Although the antigen has also been associated with ribosomal RNA transcription, it is strongly linked to cell proliferation and has thus been indicated as an effective marker in grading the proliferation rate of tumours, including those of the brain, breast, cervix, and prostate.
Anti-Hairy Cell Leukemia stains various B-cells in the follicular mantle zone and virtually all cases of hairy cell leukemia. It also stains some high grade B-cell lymphomas.
The TFE3 [IHC108] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissues within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
Transforming Growth Factor ?1 (TGF?1) is a cytokine present in regulatory T-cells, immature dendritic cells, megakaryocytes, and platelets, with a functional involvement in regulating T-cells. TGF?1 is overexpressed in thyroid cancer and cervical squamous cell carcinoma. Poor prognosis of cervical squamous cell carcinoma is associated with TGF?1.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC673
GMDN Code:
?
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Placenta
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Thyroglobulin is a precursor to the thyroid hormones T4 and T3, and is present in the thyroid follicular cells. Nearly all thyroid follicular carcinomas stain for thyroglobulin and sometimes produce a focal staining pattern. Conversely, poorly differentiated carcinomas and non-thyroid adenocarcinomas do not stain for thyroglobulin, therefore Anti-Thyroglobulin is a useful diagnostic tool for recognizing papillary and follicular thyroid carcinomas. A panel of Anti-Thyroglobulin and Anti-Calcitonin is useful for identifying medullary thyroid carcinomas, whereas a panel of Anti-Thyroglobulin and Anti-TTF1 is useful for distinguishing between primary thyroid and lung neoplasms.
Programmed Death 1 (PD-1) is a member of the CD28/CTLA-4 family of T-cell regulators, expressed as a co-receptor on the surface of activated T-cells, B-cells, and macrophages. New studies have suggested that the PD-1/PD-L1 signaling pathway may be linked to anti-tumour immunity, as PD-L1 has been shown to induce apoptosis of activated T-cells or inhibit activity of cytotoxic T-cells. In comparison to CD10 and Bcl-6, PD-1 is expressed by fewer B-cells and has therefore been considered a more specific and useful diagnostic marker for angioimmunoblastic T-cell lymphoma. Therapies targeted toward the PD-1 receptor have shown remarkable clinical responses in patients with various types of cancer, including non-small-cell lung cancer, melanoma, and renal-cell cancer.
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Country Of Origin:
Normal Cat Serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Goat anti-Dog IgG (H&L), HRP conjugated is a secondary antibody which binds to dog IgG heavy and light chain in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Reconstituted material can be stoored in 4°Cup to 7 days.
Host Animal:
Goat
Species Reactivity:
Dog IgG
Immunogen:
Purified normal IgG isolated from pooled dog serum
Applications:
Dot-blot (Dot), ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (paraffin) (IHC), Western blot (WB)
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail. No preservative added.The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion.
Application Details:
1 : 1000-1 : 10 000 (ELISA), 1 : 100 and 1 : 500 (ICC), (IHC), The optimal working dilution should be established by titration by the end user, Excess labelled antibody should be avoided since it may cause high unspecific background staining and interfere with the specific signal
Purity:
Protein G purified goat IgG in PBS pH 7.2.
Reconstitution:
For reconstitution add 1 ml of sterile destilled water
Not reactive in:
This antibody does not react with any non-Ig protein in dog serum, as tested by immunoelectrophoresis and double radial immunodiffusion
Goat anti-Dog IgG (H&L), is a secondary antibody which binds to dog IgG heavy and light chain in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Reconstituted material can be stoored in 4°Cup to 7 days.
Host Animal:
Goat
Species Reactivity:
Dog IgG (heavy & light chains)
Immunogen:
purified dog normal IgG isolated from pooled dog serum
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail. No preservative added.The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion.
Application Details:
Immunoprecipitation, In immunoelectrophoresis use 2 ml or equivalent against 120 ml antiserum, In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ml antiserum in a 3 mm diameter centre well and 2 ml serum samples (neat and diluted) in 2 mm diameter peripheral wells, Precipitating titre not less than 1:256 when tested against normal dog serum in agar block titration
Purity:
Protein G purified goat IgG in PBS pH 7.2.
Reconstitution:
For reconstitution add 1 ml of sterile destilled water
Not reactive in:
This antibody does not react with any non-Ig protein in dog serum, as tested by immunoelectrophoresis and double radial immunodiffusion
Goat anti-Dog IgG (H&L), Biotin conjugated is a secondary antibody which binds to dog IgG heavy and light chain in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Reconstituted material can be stoored in 4°Cup to 7 days.
Host Animal:
Goat
Species Reactivity:
Dog IgG
Immunogen:
Purified normal IgG isolated from pooled dog serum
Applications:
Dot-blot (Dot), ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (paraffin) (IHC), Western blot (WB)
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail. No preservative added.The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion.
Application Details:
1 : 1000-1 : 10 000 (ELISA), 1 : 100-1 : 500 (ICC, IHC), The optimal working dilution should be established by titration by the end user, Excess labelled antibody should be avoided since it may cause high unspecific background staining and interfere with the specific signal
Purity:
Protein G purified goat IgG in PBS pH 7.2.
Reconstitution:
For reconstitution add 1 ml of sterile destilled water
Not reactive in:
This antibody does not react with any non-Ig protein in dog serum, as tested by immunoelectrophoresis and double radial immunodiffusion
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Storage:
2-8 °C
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
BSA-Biotin, KLH-Biotin
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20°C.
Specificity:
This antibody reacts with Biotin
Cross Reactivity:
This antibody does not react with free BSA or free KLH
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Trademark:
DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
BSA-Biotin, KLH-Biotin
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20°C.
Specificity:
This antibody reacts with Biotin
Cross Reactivity:
This antibody does not react with free BSA or free KLH
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Trademark:
DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Immunobeads are coupled with antibodies that allows for exosome isolation from human biofluids (tested for plasma, serum, urine). Latex immunobeads can be easily recovered after incubation using a centrifuge. Exosomes captured on immunobeads can be used directly protein and mRNA/miRNA profiling.
Starting material: Recommended starting sample volume from 0.5ml of sample up to 1ml. Unfractionated plasma sample can be directly used for capture. Concentrated urine samples are recommended prior capture according to our suggested protocol.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Immunobeads are coupled with antibodies that allows for exosome isolation from cell supernatants of human cell lines. Latex immunobeads can be easily recovered after incubation using a centrifuge. Exosomes captured on immunobeads can be used directly protein and mRNA/miRNA profiling.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Immunobeads are coupled with antibodies that allows for exosome isolation from human biofluids (tested for plasma, serum, urine). Latex immunobeads can be easily recovered after incubation using a centrifuge. Exosomes captured on immunobeads can be used directly protein and mRNA/miRNA profiling.
Starting material: Recommended starting sample volume from 0.5ml of sample up to 1ml. Unfractionated plasma sample can be directly used for capture. Concentrated urine samples are recommended prior capture according to our suggested protocol.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Immunobeads are coupled with antibodies that allows for exosome isolation from cell supernatants of human cell lines. Latex immunobeads can be easily recovered after incubation using a centrifuge. Exosomes captured on immunobeads can be used directly protein and mRNA/miRNA profiling.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Immunobeads are coupled with antibodies that allows for exosome isolation from cell supernatants of human cell lines. Latex immunobeads can be easily recovered after incubation using a centrifuge. Exosomes captured on immunobeads can be used directly protein and mRNA/miRNA profiling.
Starting material: Concentrated cell culture supernatant samples (10X in spin concentrator) are recommended prior capture according to our suggested protocol.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Immunobeads are coupled with antibodies that allows for exosome isolation from cell supernatants of human cell lines. Latex immunobeads can be easily recovered after incubation using a centrifuge. Exosomes captured on immunobeads can be used directly protein and mRNA/miRNA profiling.
Starting material: Concentrated cell culture supernatant samples (10X in spin concentrator) are recommended prior capture according to our suggested protocol.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Immunoglobulin A (IgA) plays a critical role in mucosal immunity. It is present in the mucosal secretions such as tears, saliva, colostrum, intestinal juice, vaginal fluid, and secretions from the prostate and respiratory epithelium, and represents a key first line of defense against invasion by inhaled and ingested pathogens at the vulnerable mucosal surfaces. It is also found in small amounts in blood. Because it is resistant to degradation by enzymes, secretory IgA can survive in harsh environments such as the digestive and respiratory tracts, to provide protection against microbes that multiply in body secretions. It is useful when identifying multiple myeloma.
Monosan Range:
MONOSAN Ready To Use
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Ansari NA, et al. Asian Pac J Cancer Prev. 2007; 8:593-6
The antibody s a useful marker for staining many carcinomas. It stains normal and neoplastic cells from various tissues, including mammary epithelium, sweat glands and colorectal carcinoma. Hepatocellular carcinoma, adrenal carcinoma and embryonal carcinomas are consistently EMA negative, so keratin positivity with negative EMA favors one of these tumors. EMA is frequently positive in meningioma, which can be useful when distinguishing it from other intracranial neoplasms such as schwannomas.
Antibody Isotype:
IgG2a-k, IgG1-k
Monosan Range:
MONOSAN Ready To Use
Clone:
E29
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Dearnaly DP, et al. Lancet 1983; 1271-4
References 2:
Attanoos RL, et al. Histopathology. 2003; 43:231-8
The antibody detects a formalin-resistant epitope that is expressed by Reed-Sternberg cells in classic Hodgkin lymphoma, the majority of anaplastic large cell lymphomas, primary cutaneous CD30 positive T-cell lymphoproliferative disorders and in embryonal carcinomas. Occasionally diffuse large B-cell lymphoma stains with this antibody. This antibody also stains plasma cells in paraffin-embedded tissue as well as reactive immunoblasts. The staining pattern of anti-CD30 in lymphoma and embryonal carcinoma is different, with the former being membranous and exhibiting Golgi zone accentuation in location, and the latter being membranous only.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN Ready To Use
Clone:
Ber-H2
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Schwarting R, et al., Blood 1989 (74):1678-1689
References 2:
Fonatsch C, et al., Genomics 1992 (14):825-826
References 3:
George DH et al. Am J Surg Pathol. 2003 Apr;27(4): 487-93
References 4:
Hedvat CV et al. Hum Pathol. 2002 Oct;33(10): 968-74
The antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Muscle Specific Actin is a part of the actin family of proteins which are highly conserved, major components of the cytoskeleton. Anti-Muscle Specific Actin immunohistochemical reactivity is seen in skeletal, cardiac, and smooth muscle cells and can be seen in neoplasms with muscle differentiation such as leiomyomas and rhabdomyosarcomas. In contrast, antiMuscle Specific Actin reactivity is typically not seen in endothelial cells, connective tissues, carcinomas, melanomas, lymphomas and most nonmyogenic sarcomas
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN Ready To Use
Clone:
HHF35
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Gown AM, et al. Am J Pathol. 1986; 125:191
References 2:
Schmidt RA, et al. Am J Pathol. 1988; 131:19-28
References 3:
Azumi N, et al.Mod Pathol. 1988; 1:469-74
References 4:
Rangdaeng S, et al. Am J Clin Pathol. 1991; 96:32-45
CD20 is a transmembrane protein in late B-cell precursors and mature B-cells that plays a role in regulating proliferation and differentiation. CD20 expression is lost at the plasma cell stage of differentiation. MON 3226 (pan B-cell) has rarely been detected in T-cell malignancies, and is a dependable marker of B-cell lymphomas such as DLBCL. CD20 expression is present in some thymomas. It does not cross-react with non-hematopoietic neoplasms.
Antibody Isotype:
IgG2a-k
Monosan Range:
MONOSAN Ready To Use
Clone:
L26
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Country Of Origin:
Normal Hamster Serum was obtained from China.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Goat anti-human kappa light chain , DyLight 594 Conjugate is a secondary antibody conjugated to DyLight 594, which binds to all human kappa light chain in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase human kappa light chain.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0,5 mg of antibody in 0,55 ml of sterile water add 0,55 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: kappa light chains on human immunoglobulins.No reactivity is observed to: non-immunoglobulin human serum proteins heavy chains on human immunoglobulins lambda light chains on human immunoglobulins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Phospholipase C-gamma2 (PLCG2) PLCG2 is an enzyme mainly expressed in immune cells (including microglia) which are involved in innate immunity. It is involved in the transmembrane transduction of immune signals that determine the fate and function of various immune cell types.
PLCG2 has higher expression levels in pathologically afected brain regions in Alzheimers disease indicating that the immune system may play a key role in the development of Alzheimers disease and a polymorphism in phospholipase C-gamma 2 (PLCG2) has been reported to be protective against late onset Alzheimers disease (LOAD) [1].
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal Antibody
Format:
100 µg in 100 µl Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
Storage Temp:
Store at -20°C. Avoid freeze / thaw cycles.
Host Animal:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Expected Species:
Cat, Dog, Chimpanzee, Pig, Bovine
Immunogen:
Partial length recombinant human PLCG2 from the N-terminal region
If you would like further information regarding the immunogen used in the production of this antibody or have a query about if this antibody will bind to your protein/species please contact us and we can do the analysis for you.
[1] Sims R, van der Lee SJ, Naj AC, Bellenguez C, Badarinarayan N, Jakobsdottir J et al (2017) Rare coding variants in PLCG2, ABI3, and TREM2 implicate microglial-mediated innate immunity in Alzheimers disease. Nat Genet 49:13731384.
Superoxide dismutase 1 (SOD1) is one of three superoxide dismutases with SOD1 being the principal cytoplasmic superoxide dismutase in humans. It binds copper and zinc ions for its activity and stability and plays a major role in redox potential regulation. It catalyses the transformation of the superoxide anion (O2?) into hydrogen peroxide. SOD1 variants are a common cause of familial amyotrophic lateral sclerosis (ALS).
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal
Format:
100 µg in 100 µl PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
If you would like us to check if this antibody is likely to bind to this protein from a different species please contact us. We are happy to check for you.
If you would like further information regarding the immunogen used in the production of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
Antibody Isotype:
IgG
Application Details:
WB 1:500-2000. IHC 1:50-200.
Category:
Primary Antibodies
Other names:
ALS, ALS1, HEL-S-44, IPOA, SOD, hSod1, homodimer
Research Areas:
Neuroscience
NS Reagents Product Area:
Neuroscience
Molecular Weight:
16kDa (Intended as a general guide and does not allow for all isoforms and species variations)
Lysosomal acid glucosylceramidase (GBA or Glucocerebrosidase) is the lysosomal hydrolase that hydrolyzes glucosylceramide (GC) and glucosylsphingosine (GS) to ceramide and sphingosine. It is a 536-amino-acid membrane-associated protein with a 39-amino-acid leader sequence that is cleaved to produce a 497-amino-acid mature protein.
Mutations in the GBA gene cause Gaucher disease, a lysosomal storage disease characterised by an accumulation of glucocerebrosides. Patients with Gaucher disease and heterozygous carriers are at increased risk of developing Parkinson's disease and Dementia with Lewy Bodies.
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal
Format:
100 µg in 100 µl PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
If you would like further information regarding the immunogen used in the production of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
Histamine is an important messenger molecule and neuromodulator with four known G protein-coupled receptors (GPCRs), H1, H2, H3 and H4. Histamine receptor H3 (HRH3) is a presynaptic autoreceptor that regulates histamine release from histaminergic neurons via negative feedback [1] as well as a heteroreceptor that regulates the release of other neurotransmitters [2,3,4]. It is also expressed postsynaptically in the modulation of dopamine signalling.
HRH3 is expressed predominantly in the cortex, thalamus, hypothalamus, hippocampus, amygdala and basal ganglia. Dysregulated central histaminergic signalling has been associated with a number of psychiatric disorders including sleep disorders, ADHD, Alzheimer's disease, Parkinson's disease and schizophrenia.
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal Antibody
Format:
100 µg in 100 µl Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
Storage Temp:
Store at -20°C. Avoid freeze / thaw cycles.
Host Animal:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Expected Species:
Guinae Pig, Cat
Immunogen:
A synthetic peptide from the C-terminal region of human HRH3
If you would like further information regarding the immunogen used in the production of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
[1] Morisset S, Rouleau A, Ligneau X, Gbahou F, Tardivel-Lacombe J, Stark H, Schunack W, Ganellin CR, Schwartz JC, Arrang JM. High constitutive activity of native H3 receptors regulates histamine neurons in brain. Nature. 2000; 408:860-4. [2] Schlicker E, Betz R, Gothert M. Histamine H3 receptor-mediated inhibition of serotonin release in the rat brain cortex. Naunyn Schmiedebergs Arch Pharmacol. 1988; 337:588-90. [3] Schlicker E, Fink K, Hinterthaner M, Gothert M. Inhibition of noradrenaline release in the rat brain cortex via presynaptic H3 receptors. Naunyn Schmiedebergs Arch Pharmacol. 1989; 340:633-8. [4] Schlicker E, Fink K, Detzner M, Gothert M. Histamine inhibits dopamine release in the mouse striatum via presynaptic H3 receptors. J Neural Transm Gen Sect. 1993; 93:1-10.
TREM2 (Triggering receptor expressed on myeloid cells 2) is a cell surface transmembrane glycoprotein with an immunoglobulin like extracellular domain and a cytoplasmic tail [1]. It is expressed in myeloid cells including dendritic cells, granulocytes, and tissue-specific macrophages such as osteoclasts. In the brain, TREM2 is only expressed by microglia and in the central nervous system expression of TREM2 varies with higher expression in the hippocampus, spinal cord and white matter.
TREM2 plays a complex role in neuroinflammation with expression of TREM2 being upregulated in pathological conditions such as Parkinson's disease, Amyotrophic lateral sclerosis (ALS), stroke, traumatic brain injury and Alzheimer's Disease [2]. TREM2 has also been shown to have a role in potential mechanisms linking urban air pollution to Alzheimer's Disease through its involvement in the regulation of neuroinflammation [3].
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal
Format:
100 µg in 100 µl PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
If you would like us to check if this antibody is likely to bind to this protein from a different species please contact us. We are happy to check for you.
Immunogen:
KLH conjugated synthetic peptide from the N-terminal region of human TREM2
If you would like further information regarding the immunogen used in the production of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
TREM-2, TREM2a, TREM2b, TREM2c, Trggering receptor expressed on myeloid cells 2, Trggering receptor expressed on myeloid cells 2a, Triggering receptor expressed on monocytes 2
Research Areas:
Neuroscience
NS Reagents Product Area:
Neuroscience
Molecular Weight:
25kDa (Intended as a general guide and does not allow for all isoforms and species variations)
Subcellular location:
Cell membrane, Secreted
Purification:
Affinity purification
References:
[1] Bouchon A, Dietrich J, Colonna M. Cutting edge: inflammatory responses can be triggered by TREM-1, a novel receptor expressed on neutrophils and monocytes. J Immunol. 2000;164:49915.
[2] Gratuze, M., Leyns, C.E.G. & Holtzman, D.M. New insights into the role of TREM2 in Alzheimers disease. Mol Neurodegeneration 13, 66 (2018).
[3] Hendrik J. Greve, Christen L. Mumaw, Evan J. Messenger, Prasada R. S. Kodavanti, Joyce L. Royland, Urmila P. Kodavanti and Michelle L. Block. Diesel exhaust impairs TREM2 to dysregulate neuroinflammation. J Neuroinflammation. 2020; 17: 351.
CD38 is a widely expressed 45 kDa transmembrane glycoprotein with a short cytoplasmic tail (aa 121), a transmembrane domain (aa 2242) and an extracellular domain (aa 43300) [1]. It is a multifunctional ectoenzyme catalysing multiple reactions and also has a role in signal transduction and calcium signaling.
In the brain CD38 is expressed in neurons, astrocytes and microglial cells and expression was found to increase under neuroinflammatory conditions indicating that it may have a role in the regulation of neuroinflammation. In Alzheimers disease, CD38 immunoreactivity is seen in intracellular tangles and neuropil threads [2].
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal Antibody
Format:
100 µg in 100 µl Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
Storage Temp:
Store at -20°C. Avoid freeze / thaw cycles.
Host Animal:
Rabbit
Species Reactivity:
Human, Rat
Immunogen:
A synthetic peptide from the C-terminal region of human CD38
If you would like further information regarding the immunogen used in the production of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
[1] Malavasi, F.; Deaglio, S.; Funaro, A.; Ferrero, E.; Horenstein, A.L.; Ortolan, E.; Vaisitti, T.; Aydin, S. Evolution and Function of the ADP Ribosyl Cyclase/CD38 Gene Family in Physiology and Pathology. Physiol. Rev. 2008, 88, 841886. [2] Otsuka K., Mizuguchi M., Aizawa T., Haga S., Sato M., Inoya H., Namba Y., Machinami R. Immunoreactivity in Alzheimers neurofibrillary tangles (abstract) Brain Pathol. 1994;4:558.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Donkey
Immunogen:
Purified Rabbit IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20°C.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rabbit IgG · light chains on all rabbit immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rabbit serum proteins · IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat or sheep
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Trademark:
DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Donkey
Immunogen:
Purified Rabbit IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20°C.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rabbit IgG · light chains on all rabbit immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rabbit serum proteins · IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat or sheep
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Trademark:
DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Human IgG, Fc fragment
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on human IgG
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin human serum immunoglobulins · light chains on all human immunoglobulins · human IgA or IgM
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Host:
Goat
Immunogen:
Purified Mouse IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum immunoglobulins · serum proteins from bovine, horse, human rabbit or swine
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Human IgG, Fc fragment
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on human IgG
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin human serum immunoglobulins · light chains on all human immunoglobulins · human IgG F(ab)2 fragment · serum proteins from bovine, horse, mouse, or rabbit
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified using solid phase Guinea Pig IgG
Purity:
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Guinea Pig IgG, whole molecule
Buffer:
PBS, 1% BSA & 0.1% proclin 150
Preservative:
0.1% (v/v) Kathon CG
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on guinea pig IgG · light chains on all guinea pig immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin guinea pig serum immunoglobulins · IgG from bovine, chicken, goat, hamster, horse, human, mouse, rabbit, rat or sheep
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Alpha-fetoprotein (AFP) is present in fetal plasma, and it binds e.g. copper, nickel, and bilirubin. Measuring of alpha-fetoprotein level in amniotic fluid can reveal severe fetal defects. In adults, elevated AFP concentrations in the plasma can indicate hepatocellular carcinoma or teratoblastoma. In some individuals, hereditary persistance of alpha-fetoprotein can be observed without any obvious pathology.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
AFP-01
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Alpha-fetoprotein (AFP) is present in fetal plasma, and it binds e.g. copper, nickel, and bilirubin. Measuring of alpha-fetoprotein level in amniotic fluid can reveal severe fetal defects. In adults, elevated AFP concentrations in the plasma can indicate hepatocellular carcinoma or teratoblastoma. In some individuals, hereditary persistance of alpha-fetoprotein can be observed without any obvious pathology.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
AFP-11
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Antibody Diluent is developed for dilution of primary antibodies. Antibodies diluted with Antibody Diluent are primarily used in immunohistochemistry with formalin-fixed paraffin-embedded tissue sections, but also with frozen, HOPE-fixed, and cytological samples as well as in immunoblot procedures.
Antibody diluents used in immunohistochemistry should protect the antibody from microbial contamination and stabilize the antibody chemically. Antibody Diluent reduces non-specific binding of antibodies to tissue sections and is therefore extremely useful in receiving background-free staining results.
Principle of method:
Immunohistochemical staining procedures often start with incubation of a blocking solution to reduce unspecific binding of primary antibody to tissue sections. This step can be omitted if the antibody used is diluted in Antibody Diluent. Antibody Diluent ? minimises unspecific binding of the primary antibody to the tissue section, ? reduces surface tension of the antibody solution and improves spreading the reagent on the slide, ? increases microbial and chemical stability of the antibody, ? reduces adhesion of antibody to the surface of the vial, ? and minimises the danger of antibody degradation by proteolytic enzymes.
Reagents provided:
500 ml Antibody Diluent (ready-to-use)
Storage and handling:
The solution should be stored at 2-8°C without further dilution. Do not freeze it. Under these conditions the solution is stable up to the expiry date indicated on the label. Do not use product after the expiry date. If stored at room temperature the solution is stable for at least 10 month from the date of delivery. A positive and a negative control have to be carried out in parallel to the test material. If you observe unusual staining or other deviations from the expected results which could possibly be caused by this reagent, please contact our technical support.
Expected results:
During the reaction of the substrate with horse radish peroxidase or alkaline phosphatase in the presence of a chromogen, a coloured precipitate is formed at the location of the bound primary antibody. This reaction only takes place if the target antigen is existent in the tissue. The chromogen used determines the colour of the precipitate. The analysis is carried out using a light microscope.
Trouble shooting:
If you observe unusual staining or other deviations from the expected results please read these instructions carefully, contact our technical support. Also refer to the instructions of the detection systems containing Peroxide Block for guidance on general troubleshooting
Quality Control:
We recommend carrying out a positive and a negative control with every staining run. The positive control permits the validation of appropriate processing of the sample. If the negative control has a positive result, this points to unspecific staining. Please refer to the instructions of the detection system for guidance on general quality control procedures.
Performance characteristics:
Studies have been conducted to evaluate the performance of the kit reagents. The product has been found to be suitable for the intended use
Limitations of procedure:
Immunohistochemistry is a complex technique involving both histological and immunological detection methods. It requires a highly trained histotechnologist. Tissue processing and handling prior to immunostaining, for example variations in fixation and embedding or the inherent nature of the tissue can cause inconsistent results (Nadji and Morales, 1983). Inadequate counterstaining and mounting can influence the interpretation of the results. Sanbio guarantees that the product will meet all requirements described from its shipping date until its expiry date, as long as the product is correctly stored and utilized. No additional guarantees can be given. Under no circumstances shall Sanbio be liable for any damages arising out of the use of the reagent provided.
Precautions:
Use through qualified personnel only. Wear protective clothing to avoid contact of reagents and specimens with eye, skin and mucous membranes. If reagents or specimens come in contact with sensitive area, wash with large amounts of water. Microbial contamination of the reagent must be avoided, since otherwise non-specific staining may occur. ProClin300 and sodium azide (NaN3) are used for stabilisation. Reaction of sodium azide with lead or copper in drainage pipes can result in the formation of highly explosive metallic azides. Discard the antibody solution in a large volume of running water to avoid formation of deposits. A material safety data sheet (MSDS) for the pure substances is available upon request.
Donkey anti-Rabbit IgG (H&L), Biotin conjugated, min. cross-reactivity to mouse IgG or serum proteins is a secondary antibody conjugated to biotin, which binds to rabbit IgG in immunological assays.
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Rabbit IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on rabbit IgG • light chains on all rabbit immunoglobulins .Based on IEP, no reactivity is observed to: • non-immunoglobulin rabbit serum proteins • mouse IgG or serum proteins .
Herpes simplex Vvrus (HSV) is a virus that manifests itself in two common viral infections. There are actually two types of herpes simplex virus, HSV1 and HSV2. These are very similar in many ways, and both can cause either oral herpes or genital herpes. HSV1 - most commonly develops into oral herpes infecting the lips (fever blisters or cold sores). HSV1 can also infect the genital area causing sores to develop. HSV2 - generally infects the genital area (genital herpes); however, HSV2 can also infect the mouth.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
T96
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Prostate-specific antigen (PSA) is a protein produced by normal prostate cells. This enzyme participates in the dissolution of the seminal fluid coagulum and plays an important role in fertility. The highest amounts of PSA are found in the seminal fluid; some PSA escapes the prostate and can be found in the serum. This serum component has been used to track the response to therapy in men with prostate cancer.SpecificityThe mouse monoclonal antibody GCP-04 recognizes amino acids 100-104 of extracellular domain of denaturated glutamate carboxypeptidase II (PSMA, NAALADase, FOLH1), an approximately 95-110 kDa transmembrane glycoprotein.Application detailsWestern blotting: Positive material: seminal plasma, reducing conditions, recommended antibody dilution: 2-5 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
A67-B/E3
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Thyrotropin (hTSH) promotes the growth of the thyroid gland in the neck and stimulates it to produce more thyroid hormones. hTSH is composed of two subunits - alpha nad beta.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
TSH-116
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Thyrotropin (hTSH) promotes the growth of the thyroid gland in the neck and stimulates it to produce more thyroid hormones. hTSH is composed of two subunits - alpha nad beta.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
TSH-51
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Choriogonadotropin (hCG) is a protein of the molecular weight about 40 kDa. It belongs to the glycoprotein hormone family together with lutropin (LH), follitropin (FSH) and thyrotropin (TSH). Choriogonadotropin is synthesised by trophoblastic cells of the placenta during pregnancy and stimulates the growth of corpus luteum. The other hormones are produced by anterior pituitary gland. All these hormones are structurally related, being composed of two noncovalently associated subunits alpha and beta.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
HCG-61
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Herpes simplex virus (HSV) is a virus that manifests itself in two common viral infections. There are actually two types of herpes simplex virus, HSV1 and HSV2. These are very similar in many ways, and both can cause either oral herpes or genital herpes. HSV1 - most commonly develops into oral herpes infecting the lips (fever blisters or cold sores). HSV1 can also infect the genital area causing sores to develop. HSV2 - generally infects the genital area (genital herpes); however, HSV2 can also infect the mouth.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
T303
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Herpes simplex virus (HSV) is a virus that manifests itself in two common viral infections. There are actually two types of herpes simplex virus, HSV1 and HSV2. These are very similar in many ways, and both can cause either oral herpes or genital herpes. HSV1 - most commonly develops into oral herpes infecting the lips (fever blisters or cold sores). HSV1 can also infect the genital area causing sores to develop. HSV2 - generally infects the genital area (genital herpes); however, HSV2 can also infect the mouth.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
T111
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
SpecificityThe antibody 2-25LE reacts with Lewis<sup>b</sup> blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, detected on the surface of erythrocytes, certain epithelial cells, and in secretions of certain individuals.Application detailsImmunohistochemistry (paraffin sections): Recommended dilution: 5 ?g/ml; positive control: colorectal adenocarcinoma; heat mediated antigen retrieval (sodium citrate); mAb incubation 1 hour / RT, detection DAB.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
7LE
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
SpecificityThe antibody IVA30 reacts with an extracellular epitope on CD41/CD61 complex (bovine), one of the earliest markers of the megakaryocytic lineage. CD41/CD61 complex acts as the receptor for fibrinogen, fibronectin, Von Willebrand factor, vitronectin, thrombin and mediates platelets aggregation. CD41/CD61 is expressed only by platelets and megakaryocytes; the complex may be absent or strongly reduced in Glanzmann's thrombasthenia (GT). This antibody crossreacts with human platelets.Application detailsImmunohistochemistry (paraffin sections): Recommended dilution: 5-10 ?g/ml; positive control: colorectal adenocarcinoma; heat mediated antigen retrieval (sodium citrate); mAb incubation: 1 hour / RT; detection DAB.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
2-25LE
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Store up to 1 year at 4°C >>> Storage of reconstituted exosomes: Stored at -20°C for up to one month or at -80°C for up to 6 months. Recommended to avoid repeated freeze-and-thraw cycles.. Avoid repeated freeze-and-thaw cycles.
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Store up to 1 year at 4°C >>> Storage of reconstituted exosomes: Stored at -20°C for up to one month or at -80°C for up to 6 months. Recommended to avoid repeated freeze-and-thraw cycles.. Avoid repeated freeze-and-thaw cycles.
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Store up to 1 year at 4°C >>> Storage of reconstituted exosomes: Stored at -20°C for up to one month or at -80°C for up to 6 months. Recommended to avoid repeated freeze-and-thraw cycles.. Avoid repeated freeze-and-thaw cycles.
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Store up to 1 year at 4°C >>> Storage of reconstituted exosomes: Stored at -20°C for up to one month or at -80°C for up to 6 months. Recommended to avoid repeated freeze-and-thraw cycles.. Avoid repeated freeze-and-thaw cycles.
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Store up to 1 year at 4°C >>> Storage of reconstituted exosomes: Stored at -20°C for up to one month or at -80°C for up to 6 months. Recommended to avoid repeated freeze-and-thraw cycles.. Avoid repeated freeze-and-thaw cycles.
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Store up to 1 year at 4°C >>> Storage of reconstituted exosomes: Stored at -20°C for up to one month or at -80°C for up to 6 months. Recommended to avoid repeated freeze-and-thraw cycles.. Avoid repeated freeze-and-thaw cycles.
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Store up to 1 year at 4°C >>> Storage of reconstituted exosomes: Stored at -20°C for up to one month or at -80°C for up to 6 months. Recommended to avoid repeated freeze-and-thraw cycles.. Avoid repeated freeze-and-thaw cycles.
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Store up to 1 year at 4°C >>> Storage of reconstituted exosomes: Stored at -20°C for up to one month or at -80°C for up to 6 months. Recommended to avoid repeated freeze-and-thraw cycles.. Avoid repeated freeze-and-thaw cycles.
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
The Neurotensin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat amygdala using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/200-1/400 in PBS/0.3% Triton x-100 - Cy3 Technique and 1/4000-1/8000 in PBS/0.3% Triton x-100 - Bn/Av-HRP Technique. Staining is completely eliminated by pretreatment with 10 µg of Neurotensin per 1 mL of diluted antibody.
The antibody has a proven strong indirect immunofluorescent staining at a 1/400-1/600 dilution and a proven 4+ biotin-streptavidin/HRP staining at 1/1,000-1/1,200 dilution in rat globus pallidus and amygdala. Staining is completely eliminated by pretreatment with 100 µg of methionine enkephalin per mL of diluted antiserum. Pretreatment with 100 µg of leucine enkephalin only partially blocks staining.
Llama IgG - AffinityPrep Resin is a resin suitable for removal of proteins recognizing Llama IgG from liquid solutions, including traditional Immunoabsorptions and Immunoprecipitation protocols.
Product Type:
Antibody
Format:
Liquid
Storage Temp:
2-8 °C, this product should not be frozen. The shelf lite is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
Purified Llama IgG coupled to 4% agarose beads in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2. Contains 0.05 % sodium azide.
Special application note:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05% (w/v) Sodium Azide.This resin is suitable for removal of proteins recognizing Llama IgG from liquid solutions, including traditional Immunoabsorptions and Immunoprecipitation protocols.8-10 mg protein/ml resin
DAB Substrate kit is intended for immunohistochemical and in situ-hybridisation staining procedures with horse radish peroxidase (HRP). DAB (3,3-Diaminobenzidine) leads to the formation of a brown precipitate at the location of the target antigen or target nucleic acid. The precipitate is insoluble in aqueous and organic solvents and can be observed by light microscopy.
30 ml DAB Chromogen (liquid DAB concentrate) 500 ml DAB Substrate Buffer
Storage and handling:
The solutions should be stored at 2-8°C without fur ther dilution. Please store the reagents in a dark place and do not freeze them. Under these conditions the solutions are stable up to the expiry date indicated on the label. Do not use product after the expiry date. The working solution should be prepared freshly at the day of use. Once the two reagents are combined, the resulting solution can be used for up to six hours. Excess working solution needs to be disposed as hazardous substance. A positive and a negative control have to be carried out in parallel to the test material. If you observe unusual staining or other deviations from the expected results which could possibly be caused by the kit reagents please contact our technical support.
Reagent preparation:
Add 50 µl DAB Chromogen (DAB concentrate) to 1 ml of DAB Substrate Buffer and mix thoroughly. Note: Typical working concentrations are 50 µl (0.9 mg) DAB per ml substrate buffer. The colour intensity can be adjusted by decreasing or increasing the DAB concentration in the working solution. Maximum sensitivity in immunohistochemical staining can be achieved by working concentrations of about 80 µl (1.5 mg) DAB per ml substrate buffer.
Procedure:
1) Rinse the slide with wash buffer after the previous incubation step. 2) Apply the DAB working solution onto the slide. Incubate for 5-15 minutes. 3) Rinse with distilled H2O. 4) Counterstain with haematoxylin for about 30 seconds up to 5 minutes (depending on the desired staining intensity). 5) Rinse with distilled H2O. 6) Blueing in tap water for at least 5 minutes. 7) Dehydrate through a graded series of ethanol and clear in xylene. Mount with a permanent mounting medium. Note: It is also possible to mount DAB with aqueous mounting media.
Expected results:
During the reaction of the substrate with horse radish peroxidase in presence of the chromogen DAB, a brown precipitate is formed at the location of the target antigen or nucleic acid. The precipitate is insoluble in aqueous and organic solvents and can be observed by light microscopy.
Trouble shooting:
If you observe unusual staining or other deviations from the expected results please read these instructions carefully, or contact our technical support. Also refer to the instructions of the detection systems for guidance on general troubleshooting.
Quality Control:
We recommend carrying out a positive and a negative control with every staining run. The positive control permits the validation of appropriate processing of the sample. If the negative control has a positive result, this points to unspecific staining. Please refer to the instructions of the detection system for guidance on general quality control procedures.
Performance characteristics:
Studies have been conducted to evaluate the performance of the kit reagents. The product has been found to be suitable for the intended use
Limitations of procedure:
Immunohistochemistry is a complex method in which histological as well as immunological detection methods are combined. Tissue processing and handling prior to immunostaining, for example variations in fixation and embedding or the inherent nature of the tissue can cause inconsistent results (Nadji and Morales, 1983). In some tissues endogenous peroxidase activity may cause non-specific staining. The enzyme activity should be blocked by incubation with hydrogen peroxide solution (H2O2 solution). The step is carried out before incubation with primary antibody but after dewaxing and rehydration. Background staining due to endogenous biotin can be blocked through an avidin-biotin blocking step prior to the primary antibody incubation step. Inadequate counterstaining and mounting can influence the interpretation of the results. Sanbio guarantees that the product will meet all requirements described from its shipping date until its expiry date, as long as the product is correctly stored and utilized. No additional guarantees can be given. Under no circumstances shall Sanbio be liable for any damages arising out of the use of the reagent provided.
Precautions:
Use by qualified personnel only. The DAB chromogen is hazardous to your health. Wear protective clothing to avoid contact of reagents or specimen with eye, skin or mucous membrane. In case of a reagent or specimen coming into contact with a sensitive area, wash the area with large amounts of water. Microbial contamination of the reagents must be avoided, since otherwise non-specific staining may occur. Material safety data sheets (MSDS) are available upon request.
Rabbit anti-Beta-synuclein Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
Beta-synuclein is a soluble cytoplasmic protein associated with synaptic vesicles and a member of the synuclein family. Mutations in alpha-synuclein cause early onset Parkinson's disease. Expression of beta synuclein may modulate alpha-synuclein aggregation found in Parkinson's disease.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS, pH 7.4. Contains no preservative.
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide (AQEAAEEPLIEPLME-C) corresponding to human _-synuclein [99-113] in the C-terminal domain conjugated via additional C-terminal Cys to Diphtheria toxoid.
Applications:
IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
WB and IHC. A dilution of 1:500 to 1:1,000 is recommended for Western blot. _-synuclein is a soluble protein of 134 amino acids and detected with 17 kDa mobility by western blotting. By IHC the antibody detects synaptic sites in human brain formaldehyde-treated frozen tissue. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Confirmed by WB using soluble mouse and human brain extracts, reactivity for major product diminished by peptide absorption. Does not detect alpha-synuclein as tested with recombinant protein and does not react with Lewy bodies in human Dementia with Lewy Bodies or Parkinson's disease brain tissue sections.
Storage:
Lyophilized at 2-8°C. After reconstitution, store at -20°C in undiluted aliquots for up to 6 months. The antibody may be stored short term at 2-2-8°C with an appropriate antibacterial agent. Avoid freeze-thaw cycles.
Bcl-2 is the best characterized protein family involved in regulation of apoptotic cell death, consisting of anti-apoptotic and pro-apoptotic members. Bcl-2 is a useful marker for identifying neoplastic cells in follicular lymphoma. Antibodies specific for the Bcl-2 protein can be used to distinguish between reactive and neoplastic follicular proliferation in lymph node biopsies.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN Ready To Use
Clone:
124
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Tsujimoto, Y. Genes Cells. 1998; 3:697-707
References 2:
Gaulard P, et al. Am J Pathol. 1992; 140: 1089-95
References 3:
Wang T, et al. APMIS. 1995; 103:655-62
References 4:
West RB, et al. Am J Clin Pathol. 2002; 117:636-43
Sheep anti-Pan-synuclein Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
Detects human alpha-, beta-, and gamma synuclein proteins. A family of homologous proteins known as alpha-, beta-, and gamma-synuclein are abundantly expressed in brain, especially in the presynaptic terminal of neurons. Although the precise function of these proteins remains unknown, alpha-synuclein has been implicated in synaptic plasticity associated with avian song learning as well as in the pathogenesis of Parkinson's disease (PD), dementia with LBs (DLB), some forms of Alzheimer's disease (AD), and multiple system atrophy (MSA).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (AKEGVVAAAEKTKQGV) as a consensus part of human alpha-, beta-, and gamma synuclein proteins conjugated to diphteria toxoid has been used as the immunogen.
Applications:
IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
IHC, WB. A concentration of 3 µg/mL is recommended for immunohistochemistry (frozen & paraffin embedded sections) and 1 µg/mL for western blot. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Overlap specific immunohistochemical staining of alpha-, beta- and gamma synucleins This antiserum recognises human and rat alpha-, beta- and gamma synucleins.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Purification:
Affinity purified and dialysed against PBS. Contains 0.02% sodium azide.
FUNCTION: Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. SUBUNIT: Heterodimer. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA. Interacts with MAFB. SUBCELLULAR LOCATION: Nucleus. INDUCTION: C-fos expression increases upon a variety of stimuli, including growth factors, cytokines, neurotransmitters, polypeptide hormones, stress and cell injury. SIMILARITY: Belongs to the bZIP family. Fos subfamily. SIMILARITY: Contains 1 bZIP domain
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Hamster,Human,Rabbit,Rat
Immunogen:
A synthetic peptide (MFSGFNADYEASSSRC; aa 2-17) conjugated to diphtheria toxoid has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from cFos protein human, rat, mouse, hamster and cat.
Applications:
IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
IH on PFA fixed, frozen tissues. Not yet tested on formalin fixed, paraffin embedded tissue but expected to react. Affinity purified antibody is extremely powerful. A concentration of 1 -5 µg/mL is recommended most uses with short (1-8 hour) incubations. If using enhanced brightfield or amplified detection methods dilutions and long primary antibody incubations dilutions will need to be increased substantially to inhibit background staining. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.<br><br>Not recommended for western blotting applications. Mouse monoclonal antibody M-1752-100 or rabbit polyclonal antibody R-1751-50 are excellent alternatives for western blotting.
Alternative Names:
Proto-oncogene protein cFOS; c-FOS
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antiserum shows a high level of specificity for cFOS confirmed by immunohostochemistry. This antiserum is known to react with rat, rabbit and hamster cFOS.
Storage:
Store lyophilized product at 2-8°C. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Nicastrin, C-terminal Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Nicastrin, a type 1 membrane glycoprotein, is an essential component of the gamma secretase complex which is critical for the cleavage of the amyloid precursor protein and other membrane proteins. Nicastrin is widely expressed in different tissue types. This antibody detects all processed forms of Nicastrin.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS, pH 7.4. Contains no preservative.
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
A synthetic peptide (C-NAKADVLFIAPREPGAVSY) corresponding to human Nicastrin [691-709] in the C-terminal region conjugated via additional N-terminal Cys to Diphtheria toxoid.
Applications:
ICC,WB
Antibody Isotype:
IgG
Application Details:
WB and IP. Suggested concentration of 3-10 µg/mL is recommended for WB. Human or mouse brain samples commonly prepared with reducing (50mM DTT), urea (2.3 M), SDS (1.5%) in 62.5 mM Tris-HCL pH 6.8 sample buffer (without boiling) heating to 50 C for 15 min. Unprocessed full length human Nicastrin is 709 amino acids, however this protein contains an N-terminal signal peptide which is considered to undergo cleavage during processing and transit to the cell plasma membrane, in addition the protein undergoes glycosylation to produce a glycoprotein of about 145 kDa apparent MW by SDS PAGE. Biosensis recommends that the optimal working dilution should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Confirmed by WB using peptide absorption.
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Rabbit anti-Nicastrin, Central region Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Nicastrin, a type 1 membrane glycoprotein, is an essential component of the gamma secretase complex which is critical for the cleavage of the amyloid precursor protein and other membrane proteins. Nicastrin is widely expressed in different tissue types. This antibody reacts with immature forms of Nicastrin. Detection of higher mol. wt. mature forms is likely to be blocked by glycosylation in this region of the protein.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS, pH 7.4. Contains no preservative.
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
A synthetic peptide (C-QGETFDYIGSSRMVYD) corresponding to human Nicastrin [331-346] in the central region conjugated via additional N-terminal Cys to Diphtheria toxoid.
Applications:
ICC,WB
Antibody Isotype:
IgG
Application Details:
WB and IP. Suggested concentration of 3-10 µg/mL is recommended for WB. Human or mouse brain samples commonly prepared with reducing (50 mM DTT), urea (2.3 M), SDS (1.5%) in 62.5 mM Tris-HCL pH 6.8 sample buffer (without boiling) heating to 50 C for 15 min. Unprocessed full length human Nicastrin is 709 amino acids, however this protein contains an N-terminal signal peptide which is considered to undergo removal/cleavage during processing and transit to the cell plasma membrane, in addition the protein undergoes glycosylation to produce a glycoprotein of about 145 kDa apparent MW by SDS PAGE. Biosensis recommends that the optimal working dilution should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Confirmed by WB using peptide absorption.
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
FUNCTION: Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. SUBUNIT: Heterodimer. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA. Interacts with MAFB. SUBCELLULAR LOCATION: Nucleus. INDUCTION: C-fos expression increases upon a variety of stimuli, including growth factors, cytokines, neurotransmitters, polypeptide hormones, stress and cell injury. SIMILARITY: Belongs to the bZIP family. Fos subfamily. SIMILARITY: Contains 1 bZIP domain
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Hamster,Human,Rabbit,Rat
Immunogen:
A synthetic peptide (MFSGFNADYEASSSRC; aa 2-17) conjugated to diphtheria toxoid has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from cFos protein human, rat, mouse, hamster and cat.
Applications:
IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
IHC: Use at an amount of 10 µg/mL with an incubation time of 1-3 days at 4ºC. This antiserum works in paraffin and 4% PFA fixed frozen sections. Penetration is the key to success. Over-fixed tissue is problematic. Not recommended for western blotting applications. Mouse monoclonal antibody M-1752-100 or rabbit polyclonal antibody R-1751-50 are excellent alternatives for western blotting. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
c-Fos; Proto-oncogene protein cFOS; cellular oncogene fos; G0/G1 switch regulatory protein 7; FOS; G0S7
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antiserum shows a high level of specificity for c-FOS confirmed by immunohistochemistry. This antiserum is known to react with rat and rabbit and hamster cFOS.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Sheep anti-Beta-synuclein Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
Beta-synuclein is a non-amyloid component of senile plaques found in Alzheimer disease. It could act as a regulator of SNCA aggregation. It protects neurons from staurosporine and 6 hydroxy dopamine -stimulated capspase activation in a p53-dependent manner. It localises to the cytoplasm and it is predominantly expressed in the brain where it is most concentrated in presynaptic nerve terminals. This protein is phosphorylated. This protein is also associated with the disease Brain iron accumulation type 1 (NBIA1).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Affinity purified and dialysed against PBS. Contains 0.02% sodium azide.
Host Animal:
Sheep
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (IEPLMEPEGSYEDPPQE) of human beta synuclein protein (aa: 108-125) conjugated to diptheria toxid has been used as the immunogen.
Applications:
IHC-Frozen
Antibody Isotype:
IgG
Application Details:
IHC. A working concentration of 1 µg/mL is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
SNCB
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Known to be specific for beta synuclein. This antiserum is known to react with beta synuclein of human, rat and other rodents.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Sheep anti-C-reactive protein Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
C-reactive protein has several roles associated with host defence such as; promoting agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. It can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. COFACTOR: Binds 2 calcium ions per subunit. C-reactive protein exists as a homopentamer. Pentaxin (or Pentraxin) have a discoid arrangement of 5 non-covalently bound subunits. There are 2 alternatively spliced isoforms. C-reactive protein is found in plasma and its concentration increases greatly during acute phase response to tissue injury, infection or other inflammatory stimuli. It is induced by IL-1 and IL-6.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized with 0.01% Thimerosal
Host Animal:
Sheep
Species Reactivity:
Human
Immunogen:
Human recombinant C-Reactive protein
Applications:
WB
Antibody Isotype:
Mixed
Application Details:
WB. Extremely high titre antibody. For western blot a dilution of 1: 10,000 to 1: 100000 is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
CRP; PTX1
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
It has been shown to be specific for C-reactive protein by WB. Human, other species have not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent.
GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. FUNCTION: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. DISEASE: Defects in GDNF may be a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. DISEASE: Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant human GDNF
Applications:
WB
Antibody Isotype:
Mixed
Application Details:
WB. Recommended to be used at a dilution of 1:1000 to 1:3000 for Western blot. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
No cross reactivity with NTN has been observed in western blot analysis. This antibody is known to react with human, mouse and rat GDNF.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. FUNCTION: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. DISEASE: Defects in GDNF may be a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. DISEASE: Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant human GDNF
Applications:
IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
IHC, WB. A dilution of 1 µg/mL is recommended for both applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
No cross reactivity with NTN has been observed in western blot analysis. This antibody is known to react with human, mouse and rat GDNF.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Supplied in 10 mM Sodium Phosphate, 0.15 M Na/K Chloride, pH 7.2.For use as a standard or control in most immunoassay formats.This product is derived from Syrian Hamster.
Rabbit anti-Ubiquitin Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC.
Background Info:
Ubiquitin is a highly conserved 76 amino acid protein with an estimated molecular weight of 8.56 kDa which has a central role in regulated protein degradation. It is a protein modifier which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Several types of polymeric chains can be formed depending on the lysine used for the assembly. Attachment to proteins as a polymer leads to their degradation by the 26S proteosome; a complex, multicatalytic cytosolic and nuclear protease. Attachment to proteins as a monomer or as an alternatively linked polymer does not lead to proteasomal degradation and may be required for numerous functions, including maintenance of chromatic structure, regulation of gene expression, stress response, ribosome biogenesis and DNA repair. Ubiquitin is synthesized as a polyubiquitin precursor with exact head to tail repeats, the number of repeats of which differ between species and strains. In some species there is a final amino-acid after the last repeat, here in bovine a Cys. Some ubiquitin genes contain a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27a).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS, pH 7.2, 100mM NaCl, 15mM sodium azide.
Ubiquitin isolated from cow erythrocytes and conjugated to chicken gammaglobulins with glutaraldehyde.
Applications:
ICC,IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
This antibody is recommended for IHC, WB and immunoprecipitation. This antibody can be used for labelling formalin-fixed, paraffin-embedded tissue sections at a dilution range of 1:150 to 1:300. This antibody is also useful for investigating neurodegenerative diseases such as Alzheimer 's disease and Parkinson's disease. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
RPS27A; UBA52; UBB; UBC
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The specificity of this antibody has been determined by indirect ELISA against ubiquitin conjugated to keyhole limpet haemocyanin. The antibody does not react with kehole limpet haemocyanin alone. Specificity has also been demonstrated by WB against endometrial tissue homogenates. Cross reacts with human, baboon and rat ubiquitin. Other species have not been tested. Ubiquitin protein sequence is 100% conserved in all higher mammals and most eukarotes.
Storage:
After reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Nicastrin, N-terminal Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Nicastrin, a type 1 membrane glycoprotein, is an essential component of the gamma secretase complex which is critical for the cleavage of the amyloid precursor protein and other membrane proteins. Nicastrin is widely expressed in different tissue types. This antibody detects all processed forms of Nicastrin.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS, pH 7.4. Contains no preservative.
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
A synthetic peptide (RGNSVERKIYIPL-C) corresponding to human Nicastrin [32-44] in the N-terminal region conjugated via additional C-terminal Cys to Diphtheria toxoid.
Applications:
ICC,WB
Antibody Isotype:
IgG
Application Details:
WB and IP. Suggested concentration of 3-10 µg/mL is recommended for WB. Human or mouse brain samples commonly prepared with reducing (50mM DTT), urea (2.3 M), SDS (1.5%) in 62.5 mM Tris-HCL pH 6.8 sample buffer (without boiling) heating to 50 C for 15 min. Unprocessed full length human Nicastrin is 709 amino acids, however this protein contains an N-terminal signal peptide which is considered to undergo cleavage during processing and transit to the cell plasma membrane, in addition the protein undergoes glycosylation to produce a glycoprotein of about 145 kDa apparent MW by SDS PAGE. Biosensis recommends that the optimal working dilution should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Confirmed by WB using peptide absorption.
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
FUNCTION: Involved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. SUBUNIT: Homotetramer. SUBCELLULAR LOCATION: Cytoplasm. Lysosome. Also found in lung secretory organelles. MISCELLANEOUS: The active site is the redox-active Cys-47 oxidized to Cys-SOH. Cys-SOH may rapidly react with a Cys-SH of the other subunit to form an intermolecular disulfide with a concomitant homodimer formation. The enzyme may be subsequently regenerated by reduction of the disulfide by thioredoxin . MISCELLANEOUS: Irreversibly inactivated by overoxidation of Cys-47 (to Cys-SO(3)H) upon oxidative stress. SIMILARITY: Belongs to the ahpC/TSA family. Rehydrin subfamily.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Rat recombinant Peroxiredoxin-6. The sequence is homologous in human and mouse peroxiredoxin-6.
Applications:
ELISA,ICC,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB, ELISA. This antibody works superbly in Immunohistochemistry on frozen or paraffin embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for routine immunohistochemistry are 1: 100 to 1: 1000 depending on tissue and detection method. For western blotting a dilution range of 1: 1000 to 1: 4000 is recommended. A dilution of 1: 1000 to 1: 4000 is recommended for ELISA. This antiserum stains the cytoplasm of epithelial cells in the rat and mouse lung and rat and human brain astrocytes. It stains human brain astrocytes in Parkinson's and Alzheimer 's disease and the central core of some Lewy bodies in Parkinson's disease and dementia with Lewy bodies. Other tissues have not yet been tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Walsh B. et al. (2010) Overexpression of Prdx6 and resistance to peroxide-induced death in Hepa1-6 cells: Prdx suppression increases apoptosis. Redox Rep. 2009;14(6):275-84. Gardiner F. et al. (2010) Induction of Prdx1 and Prdx6 in Liver Cellsby Serum and TPA. Intl J. Biol. Vol. 2, No. 1
Specificity:
This antibody has been shown to be specific for Peroxiredoxin-6 protein. Rat, human and mouse, other species have not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
Sheep anti-Connexin-45 (Cx45) Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
Connexin-45 is a component of gap junctions, which are composed of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low molecular weight diffuse from one cell to a neighboring cell. SUBUNIT: A connexon is composed of a hexamer of connexins. SUBCELLULAR LOCATION: Membrane; multi-pass membrane protein. SIMILARITY: Belongs to the connexin family. Alpha-type (group II) subfamily. Alternatively spliced isoforms have been described.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human
Immunogen:
A synthetic peptide (QAYSHQN NPHGPRE) as part of human Connexin-45 protein (aa: 354-367) conjugated to diphtheria toxoid has been used as the immunogen.
Applications:
WB
Antibody Isotype:
Mixed
Application Details:
<b>Immunohistochemistry:</b> Antibody detects sparse Cxn45-IR in caudal artery and heart tissue (see Rummery, NM et al 2002 for more staining specifics). Antibody was used at 1:100 to 1:250, but Biosensis recommends optimal dilutions/concentrations should be determined by the end user. In the original work the specificity of the antibody was shown by incubation either without primary antibody or with primary antibody that had previously been pre-incubated for 1 hour at room temperature with 10-fold excess by weight of the peptide against which the antibody was raised.<br><br><b>Western Blot:</b> Antibody is not recommended for western blotting by Biosensis, however, it does react in westerns with Cxn 45 specific material. The authors report that the antibody develops numerous bands in westerns blots, only some of which are removed upon peptide treatment (see Rummery, NM et al 2002). The Cx45/354 antibody revealed the presence of a specific 45-kDa band in all tissues tested, although it was very weak in the arteries. A higher molecular weight band, which was blocked by peptide, was also seen in the brain (see Rummery, NM et al 2002, online Figure VIIB, -/+ peptide).
Alternative Names:
Gap junction gamma-1 protein; Gap junction alpha-7 protein; Cx45; GJC1; GJA7
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Original Reference Rummery NM, Hickey H, McGurk G, Hill CE. (2002) "Connexin37 is the major connexin expressed in the media of caudal artery. Arterioscler Thromb Vasc Biol. 22(9):1427-32. PMID: 12231561 This antibody is referred to as Cx45/354 in Rummery, NM et al 2002.
Specificity:
Western blotting of cell membranes of COS cells transiently transfected with cDNA encoding Connexin-45 shows a singular band of molecular weight 47 kDa which is abolished by preincubation with the immunising peptide. No such band is detected in western blots of membranes from non-transfected cells or cells transfected with cDNA encoding Connexin-37 or Connexin-40. This antiserum reacts to human Connexin-45.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Sheep anti-Beta-synuclein Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
Beta-synuclein is a non-amyloid component of senile plaques found in Alzheimer disease. It could act as a regulator of SNCA aggregation. It protects nerurons from staurosporine and 6 hydroxy dopamine -stimulated capspase activation in a p53-dependent manner. It localises to the cytoplasm and it is predominantly expressed in the brain where it is most concentrated in presynaptic nerve terminals. This protein is phosphorylated. This protein is also associated with the disease Brain iron accumulation type 1 (NBIA1).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (IEPLMEPEGSYEDPPQE) as part of human beta synuclein protein (aa: 108-125) conjugated to diphteria toxid has been used as the immunogen.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A dilution of 1:3000 to 1:8000 is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
SNCB
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Known to be specific for beta synuclein. This antiserum is know to react with human, rat and other rodent beta synuclein.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
SUMO-1 binds to a wide range of target proteins as part of a post-translational modification system. Unlike ubiquitin, it does not seem to target protein for degradation, but is involved in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis, apoptosis, protein stability and signal transduction. SUBUNIT: Covalently attached to a number of proteins such as PmL, RANGAP1, HIPK2, SP100, p53, p73alpha, MDM2, JUN and DNMT3B. Also interacts with HIF1A, HIPK2, HIPK3, CHD3, PIAS1, EXOSC9, TDG, RAD51 and RAD52. SUBCELLULAR LOCATION: Nucleus; nuclear membrane. Nucleus; nucleoplasm; nuclear speckle. Cytoplasm. SIMILARITY: Belongs to the ubiquitin family. SMT3 subfamily. SIMILARITY: Contains 1 ubiquitin-like domain. PTM: Cleavage of the last four amino acids of the carboxy-terminus of the precursor form by SENP1 or SENP2 is necessary for function. Several pseudogenes have been reported as well as a number of alternatively spliced isoforms.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (AKPSTEDLGDKKEGEY) as part of human SUMO-1 peptide (aa: 6-21) conjugated to diphtheria toxoid has been used as the immunogen. This antigen is homologous with SUMO-1 of rat.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB. A dilution of 1:2000 to 1:4000 is recommended for immunohistochemistry and 1:4000 to 1:8000 for western blot. Cell lysate from Hela and NIH-3T3 cell lysates may be used as a positive control, and for IHC, lung carcinoma may be used. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Small ubiquitin-related modifier 1; Ubiquitin-like protein SMT3C; SMT3 homolog 3; Ubiquitin-homology domain protein PIC1; Ubiquitin-like protein UBL1; GAP-modifying protein 1; GMP1; Sentrin; SUMO1; SMT3C; SMT3H3; UBL1
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antiserum recognises human SUMO-1 and not ubiquitin. This antiserum is known to cross react with rat and human SUMO-1.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Glutathione peroxidase 1 has a role in detoxification of hydrogen peroxide and is one of the most important antioxidant enzymes in humans. It exists as a homotetramer which localises to the cytoplasm. It belongs to the glutathione peroxidase family. Glutathione peroxidase 1 is one of few proteins in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by UGA, that normally functions as a translation termination codon. This protein has a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine repeats. The allele with five alanine repeats is significantly associated with breast cancer risk. Two alternatively spliced isoforms have been identified.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (RRYSRRFQTIDIEPDIEALL) corresponding to the amino acids 175-194 of human lutathione peroxidase 1 ( GPx-1) conjugated to diphtheria toxin has been used as the immunogen.
Applications:
ELISA,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB. This antibody works superbly in Immunohistochemistry on frozen or paraffin embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for routine immunohistochemistry are 1: 2000 to 1: 20000 depending on tissue and detection method. For western blotting a dilution range of 1: 10000 to 1: 100000 is recommended. For ELISA, a dilution of 1: 2000 to 1: 20000 is recommended. This antiserum has extremely high titre, it stains human and rat brain microglia intensely and to some extent also stains neurons. Other tissues have not yet been tested. On Western blotting under reducing conditions recognises a 22kD protein in human brain tissue and Sigma glutathione peroxidase (G-4013) isolated from human erythrocytes. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
This antibody has been shown to be specific for glutathione peroxidase 1 (GPx-1) protein. Human and rat, other species have not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
Brain derived neurotrophic factor (BDNF) is synthesized as a precursor (proBDNF) which may be released and have physiological functions to cause cell death. It binds neurotrophin receptor p75 and sortilin and may also be important for the development of nervous system. proBDNF is synthesized in neurons and glia (eg., microglia), transported anterogradely and retrogradely and may be released in an activity dependent manner. This antibody is raised in sheep to detect the prodomain of BDNF and not the mature peptide.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Mouse,Rat
Immunogen:
The recombinant prodomain fragment of human brain-derived neurotrophic factor
Applications:
ELISA,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
ELISA, Western Blot, biological neutralization of proBDNF, Immunocytochemistry/Immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Proform brain derived neurotrophic factor
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Confirmed to react with purified human proBDNF, crossreact with mouse and rat proBDNF Cross reactivity with other species than human, mouse and rat has not yet been tested
Storage:
After reconstitution keep aliquots at -20ºC for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
GPx-P belongs to the glutathione peroxidase family which are responsible for the detoxification of hydrogen peroxide. It protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. GPx-P is secreted in plasma and exists as a homotetramer.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (FEKGDVNGEKEQKFYTFLKN) corresponding to the amino acids 135-154 of human glutathione peroxidase 3 (GPx-P) conjugated to diphtheria toxin has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from glutathione peroxidase 3 (GPx-P) protein in mouse, rat, orangutan and bovine.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC, only. A dilution o f 1: 1000 to 1: 5000 is recommended for immunohistochemistry depending on detection method but end user will need to determine dilution to suit application. Antigen retrieval is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
This antibody has been shown to be specific for glutathione peroxidase 3 protein (GPx-P) through staining and blocking studies. Human and rat, other species have not yet been ested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Sheep anti-Connexin-40 (Cx40) Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
FUNCTION: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low molecular weight diffuse from one cell to a neighboring cell. SUBUNIT: A connexon is composed of a hexamer of connexins. SUBCELLULAR LOCATION: Membrane; multi-pass membrane protein. TISSUE SPECIFICITY: Highly expressed in lung. SIMILARITY: Belongs to the connexin family. Alpha-type (group II) subfamily.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Rat
Immunogen:
A synthetic peptide consisting of amino acids 254 to 270 of the C-terminus of rat Cx40 (Cx40/254) conjugated to diphtheria toxoid has been used as the immunogen.
Applications:
WB
Antibody Isotype:
IgG
Application Details:
<br><b>Immunohistochemistry</b>: Antibody detects Cxn 40 in rat tissues and arterial endothelial cells. The authors report that the density of Cx40 plaques was significantly greater in the caudal artery (CA) than in the thoracic aorta (ThA), whereas no such difference was seen for Cx37 and Cx43. Expression of Cx40 was absent from the media of both thoracic and caudal artery tissues (see Rummery, NM et al 2002 for more staining specifics). <br><b>Published Method</b>: Unfixed 10 µm thick sections cryosections or lightly fixed (2% paraformaldehyde in 0.1 mol/L sodium phosphate buffer) whole mount sections have been tested, see Rummery, NM et al 2002). Pretreatments include pre-incubation for 30 minutes in a blocking solution of 2% bovine serum albumin (BSA), 0.2% Triton-X in PBS, followed by primary antibody incubation. Antibody was used at 1:100 to 1:250 for 1 hour in the original work but Biosensis recommends optimizing the conditions for the best results. Original detection was via Cy3- conjugated anti-goat immunoglobulins (Jackson Immunoresearch Laboratories Inc, PA, 1:100) in 0.01% Triton-X in PBS, but other secondary conditions should work as well once optimized. In the original work the specificity of each antibody was tested by incubation either without primary antibody or with primary antibody that had previously been pre-incubated for 1 hour at room temperature with 10-fold excess by weight of the peptide against which the antibody was raised. (Adapted from Rummery, NM et al 2002).<br><b>Western Blot</b>: <U>Antibody is not recommended for western blots by Biosensis</u>, however, it does react in westerns with Cxn 40 specific material. The authors report that the antibody develops numerous bands in westerns blots, only some of which are removed upon peptide treatment (see Rummery, NM et al 2002). The Cx40/254 antibody specifically recognized a band of 40 kDa from lung, caudal artery (CA), and thoracic aorta (ThA) but not liver (online Figure VIIA, +/- peptide). In the lung, however, a band at 45 kDa also appeared to be reduced with Cxn 40 peptide addition.<br><b>Published Method</b>: Brain, heart, liver, lung, thoracic aorta and caudal arteries were removed from 5-6 week old Wistar rats and snap frozen in liquid nitrogen Tissues were ground under liquid nitrogen in a mortar and pestle and resuspended in 1mL of lysis buffer (1 mM NaHCO3 pH 7.05, 10 mM EDTA, 10 mM Iodoacetamide, 10 mM tetra-sodium pyrophosphate, 1 mM PMSF and 1 µg/mL each of antipain, aprotinin, pepstatin-A, chymostatin and leupeptin). Tissues were further disrupted by grinding in a polytron blender. Unbroken cells and large debris were removed by centrifugation at 1000 g for 5 minutes at 4oC, the supernatant was then removed and centrifuged at 3000 g for 5 minutes. The pellet was discarded, and the supernatant centrifuged at 20000 g for 15 minutes at 4°C. The supernatant was discarded, and the membrane-enriched pellet was resuspended in lysis buffer. Protein concentration was measured using the Bio-Rad protein assay kit. Membrane-bound connexins were subsequently solubilized by incubation in 2x SDS sample buffer (5% SDS, 125 mM Tris-Cl (pH 6.8), 20% glycerol, 2 mM _- mercaptoethanol, 0.1% (w/v) bromophenol blue) for 60 minutes at 37°C. Aliquots containing 5 µg of protein were separated by SDS-PAGE on 12% polyacrylamide gels and blotted onto PVDF membranes. Blots were probed with sheep antibodies against Cx40 (1:1000, Cx40/254) and detected via ECL using anti-Goat poly HRP secondary antibodies 1:4000, 1 hr. (<I>adapted from Rummery, NM et al 2002</I>).Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Gap junction alpha-5 protein; Cx40; Gja5; Cxn-40
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Original Reference Rummery NM, Hickey H, McGurk G, Hill CE. (2002) "Connexin37 is the major connexin expressed in the media of caudal artery. Arterioscler Thromb Vasc Biol. 22(9):1427-32. PMID: 12231561 This antibody is referred to as Cx40/254 in Rummery, NM et al 2002.
Specificity:
It is reported that the Cx40/254 antibody did not cross react with rat Cx43 but may with Cx45 in Western blots only (Rummery, NM et al 2002).
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Neuropeptide Y (NPY) Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
Neuropeptide Y is a 36 amino acid neurotransmitter found in the brain and autonomic nervous system. It has been associated with numerous physiologic processes in the brain, including the regulation of energy balance, memory and learning, and epilepsy. The main effect is increased food intake and decreased physical activity. It is also implicated in the secretion of gonadotrophin-release hormone. It is secreted by the hypothalamus and it is one of the most abundant peptides in the nervous system. It is also found in some chromaffin cells of the adrenal medulla. The sheep form of NPY is 94% homologous with human NPY.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat,Sheep
Immunogen:
Sheep Neuropeptide Y (29-64: NB. includes only the NPY peptide and not either of the flanking peptides) conjugated to keyhole limpet hemocyanin.
Corder KM et al. (2018) Prefrontal cortex-dependent innate behaviors are altered by selective knockdown of Gad1 in neuropeptide Y interneurons. PLoS One. 2018 Jul 19;13(7):e0200809 Application: IHC/IF, frozen sections.
Specificity:
Specificity has been confirmed by direct ELISA against the antigen. Sheep, Human, Mouse & Rat.
Storage:
Maintain lyophilized material at 2-8°C for up to 12 months. After reconstitution keep aliquots at -20°C for up to six months or at 2-8°C with an appropriate antibacterial agent for up to 4 weeks. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Purification:
Affinity purified on antigen column. Refer to vial label for antibody concentration after reconstitution.
Rabbit anti-Beta casein protein Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
Beta-casein has an important role in determination of the surface properties of the casein micelles. it is cleaved into 3 chains (casoparan, casohypotensin and antioxidant peptide). Casoparan acts as a macrophage activator, increasing the phagocytic activity of macrophages and peroxide release from macrophages. It also acts as a bradykinin-potentiating peptide. Casohypotensin acts as a bradykinin-potentiating peptide and induces hypotension in rats. Acts as a strong competitive inhibitor of endo-oligopeptidase A. Antioxidant peptide has antioxidant activity (ref: uniprot.org.).<br /><br />Bovine milk contains two types of beta-casein protein, A2 or A1. Recent studies have shown that milk containing the A1 beta casein protein can contribute to issues including gastrointestinal discomfort after ingestion. There is some evidence of a link between ingestion of A1 beta casein protein and the development of Type 1 diabetes.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS, pH 7.4, with 0.02% sodium azide as preservative. Refer to the product label for antibody concentration.
Host Animal:
Rabbit
Species Reactivity:
Bovine
Immunogen:
Bovine beta-casein peptides
Applications:
ELISA,WB
Antibody Isotype:
IgG
Application Details:
ELISA and western blotting (1:5,000 - 1: 20,000). Do not use skim milk for blocking and dilution of assay reagents, BSA is recommended. Biosensis recommends that optimal working dilutions should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Physical State:
Liquid.
Product references:
Indyk HE et al. (2021) The determination of intact ?-casein in milk products by biosensor immunoassay. J. Food Compos. Anal. 101:103946 Application: Immunoassay.
Specificity:
Bovine beta-casein. Detects both A1 and A2 beta-casein isoforms.
Storage:
Spin vial briefly before opening and centrifuge to remove any insoluble material. After opening maintain at -20°C in undiluted aliquots for up to 6 months. For short-term storage, keep aliquot at 2-8°C for up to one week. Avoid repeated freeze-thaw cycles.
FUNCTION: Involved in redox regulation of the cell. Can reduce hydrogen peroxide and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. SUBUNIT: Homotetramer. May interact with HTR2A. SUBCELLULAR LOCATION: Cytoplasm. Lysosome. Also found in lung secretory organelles. MISCELLANEOUS: The active site is the redox-active Cys-47 oxidized to Cys-SOH. Cys-SOH may rapidly react with a Cys-SH of the other subunit to form an intermolecular disulfide with a concomitant homodimer formation. The enzyme may be subsequently regenerated by reduction of the disulfide by thioredoxin . MISCELLANEOUS: Irreversibly inactivated by overoxidation of Cys-47 (to Cys-SO(3)H) upon oxidative stress. SIMILARITY: Belongs to the ahpC/TSA family. Rehydrin subfamily.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Rat recombinant Peroxiredoxin-6
Applications:
ELISA,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB, ELISA. This antibody works superbly in Immunohistochemistry on frozen or paraffin embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for routine immunohistochemistry are 1: 100 to 1: 1000 depending on tissue and detection method. For western blotting a dilution range of 1: 500 to 1: 2000 is recommended. A dilution of 1: 1000 to 1: 2000 is recommended for ELISA. This antiserum stains the cytoplasm of epithelial cells in the rat and mouse lung and rat and human brain astrocytes. It stains human brain astrocytes in Parkinson's and Alzheimer 's disease and the central core of some Lewy bodies in Parkinson's disease and dementia with Lewy bodies. Other tissues have not yet been tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
This antibody has been shown to be specific for Peroxiredoxin-6 protein. Rat, human and mouse, other species have not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
CNTF is a survival promoting factor for different types of neurons in vitro and in vivo. The essential structural features for the biological function of human CNTF were investigated by Thier, M. et al. They showed that deletion of 14 N-terminal and 18 C-terminal amino acids significantly increased bioactivity compared to wild-type CNTF. FUNCTION: CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. SUBUNIT: Homodimer. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Nervous system. PHARMACEUTICAL: CNTF is being tested under the name Axokine by Regeneron Pharmaceuticals for treatment of human motor neuron diseases, such as amyotrophic lateral sclerosis (ALS). As it induces substantial weight loss, preferentially of fat as opposed to lean body mass, it is being used for obesity treatment. SIMILARITY: Belongs to the CNTF family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Mixture of two peptides from human CNTF (11-25 and 186-200 aa). Both peptides are highly conserved among human and mouse.
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
WB and ELISA at a suggested dilution of 1:500 to 1:2,000. A titration between 1:50 to 1:500 is suggested for IHC. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
Ciliary neurotrophic factor; CNTF;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
CNTF, Cross reactivity to other species is expected
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Rabbit anti-Adrenocorticotropic hormone (ACTH) Polyclonal Antibody (Unconjugated), suitable for IHC-Paraffin-embedded.
Background Info:
Adrenocorticotropic hormone (ACTH) is cleaved from the precursor pro-opiomelanocortin (POMC). The hormone is produced and secreted by the pituitary gland and stimulates release of cortisol by adrenal glands.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized with BSA
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Pig,Rat
Immunogen:
Porcine Adrenocorticotropic hormone (ACTH) conjugated to BSA
Applications:
IHC-Paraffin-embedded
Antibody Isotype:
IgG
Application Details:
A dilution of 5-10 µg/mL is recommended for immunohistochemistry using formalin fixed and paraffin embedded tissues and for 4% paraformaldehyde fixed frozen tissues. A dilution of 5-15 µg/mL is recommended for immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Corticotropin; ACTH; Pro-opiomelanocortin; POMC;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human; mouse; rat; ACTH is highly conserved so cross-reactivity with other species is expected.
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 2-8°C with an appropriate antibacterial agent.Avoid freeze-thaw cycles
p75NTR (CD271) was originally discovered as a low affinity nerve growth factor receptor (NGFR). Later it was found that it was the receptor for all neurotrophins, including NGF, BDNF, NT3 and NT4/5. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. Recent research proposes the extracellular domain of p75NTR as a biomarker for monitoring the progression of motor neuron disease (MND), also known as Amyotrophic Lateral Sclerosis (ALS) or Lou Gehrig's Disease. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from a solution containing PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives.
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant extracellular domain (amino acids 29-250) of human NGFR/p75NTR protein with N-terminal His-tag.
Applications:
FC,ICC,IHC-Frozen,Immunopanning,IP,WB
Clone number:
8J2
Antibody Isotype:
IgG2a
Application Details:
Flow Cytometry: 5-20 µg/mL.<br>Western Blotting: 0.5-2.0 µg/mL, <b>non-reducing conditions only</b> (no DTT or beta-mercaptoethanol).<br>Immunoprecipitation: lysate dependent. 10 ug per 200-500 ug total protein.<br>Immunopanning: 1-5 µg/mL.<br>Immunocytochemistry: 1-5 µg/mL. Staining is strongest in non-fixed cells, light fixation is tolerable.<br>Immunohistochemistry: fresh, acetone fixed sections only, epitope is fixation sensitive. Not suitable in formalin-fixed, paraffin (FFPE) embedded tissues.<br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Hulme AJ et al. (2020) Molecular and Functional Characterization of Neurogenin-2 Induced Human Sensory Neurons . Front Cell Neurosci. 14:600895 Application: Human, ICC(IF).
Specificity:
Human, reacts with human, mouse and rat. Cross-reactivity with other species not tested but expected.This antibody is specific for NGFR/p75NTR as demonstRated by western blotting and immunprecipitation. The antibody recognizes extracellular p75NTR under non-reducing conditions.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Presenilin 1 (PS-1) Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
Presenilin-1 (PSEN1) is a multi-pass membrane protein and component of the gamma-secretase complex. PSEN1 is thought to play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. It may also play a role in hematopoiesis. Defects in PSEN1 are a cause of Alzheimer disease type 3 (AD3), a familial early-onset form of Alzheimer disease (Ref:SWISS-Prot).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS, pH 7.4. Contains no preservative.
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a region (1-20 aa) from the N-terminus of human Presenilin 1 conjugated to Diptheria toxoid.
Applications:
WB
Antibody Isotype:
IgG
Application Details:
IF and WB. Suggested dilution of 1:2,000 is recommended for WB. On SDS-PAGE, the predominant form detected by this antibody is the N-terminal Presenilin 1 fragment of approx 29 kDa. The uncleaved form of Presenilin 1 migrates to approx 45 kDa. Human and mouse brain samples commonly prepared with reducing agent (50mM DTT), urea (2.3M), SDS (1%) in 62.5 mM Tris-HCl pH 6.8 sample buffer heated to 50C for 15 min. The suggested dilution for IF is 1:100 for acetone or paraformaldehyde fixed cells or tissue. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
Presenilin 1; PS-1; Protein S182; PS1-CTF12; PSEN1; AD3; PS1; PSNL1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specificity confirmed by WB and IF using transfected cells, Presenilin 1 knock-out mouse cells, mouse and human brain.
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Mixture of two mouse beta NGF peptides (74-85 and 113-124 aa).
Applications:
ELISA,IHC-Frozen,WB
Antibody Isotype:
IgY
Application Details:
ELISA, WB and IHC. WB suggested dilution of 1:500-1:2,000. IHC suggested dilution of 1:50-1:1,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
Beta-nerve growth factor; Beta-NGF; NGF; NGFB;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human beta NGF, cross reactivity to other species NGF is expected
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Dicer is the rate limiting enzyme in the formation of mature microRNAs.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS, 0.02% sodium azide
Host Animal:
Chicken
Species Reactivity:
Mouse,Rat
Immunogen:
A synthetic peptide from mouse Dicer protein (1388-1405 aa) conjugated to KLH.
Applications:
ICC,IHC-Frozen,WB
Antibody Isotype:
IgY
Application Details:
Western Blotting (WB), Immunohistochemistry (IHC) and Immunoprecipitation (IP). The recommended concentration for IHC in formalin fixed and paraffin embedded tissues and formalin/acetone fixed tissues is 1:200-1:500. For WB, the recommended concentration is 1:1,000 to 1:3,000. Mouse Dicer Protein has a predicted length of 1916 residues and the MW of the monomer is 218 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Lugli G. et al (2008) Expression of microRNAs and their precursors in synaptic fractions of adult mouse forebrain. J Neurochem. Jul;106(2):650-61. Lugli G. et al (2005) Dicer and eIF2c are enriched at postsynaptic densities in adult mouse brain and are modified by neuronal activity in a calpain-dependent manner. J Neurochem. Aug;94(4):896-905.
Specificity:
Specificity confirmed by IHC and WB in mouse brain. Mouse; Rat;
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 2-8°C with an appropriate antibacterial agent. Avoid freeze-thaw cycles.
Chicken anti-Mitochondrial uncoupling protein 3 (UCP 3) Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
Uncoupling Protein 3 (UCP3) belongs to the mitochondrial carrier family. Located in the mitochondrion inner membrane, UCP3 creates proton leaks across the membrane thus uncoupling oxidative phosphorylation (Ref: SWISSPROT).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Pig,Rat
Immunogen:
A peptide from the C-terminus of human Uncoupling Protein 3 (298-312 aa).
Applications:
WB
Antibody Isotype:
IgY
Application Details:
WB. Suggested dilution at 1:1,000 to 1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
UCP3; Mitochondrial uncoupling protein 3; UCP 3; Solute carrier family 25 member 9; SLC25A9;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antibody detects UCP3 at approx 35 kDa. Human, Mouse, Rat, Porcine
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Transforming growth factor beta-1 (TGFB1) is a multi-functional cytokine with roles in proliferation, differentiation and other functions in many cell types. The secreted TGFB1 protein is cleaved into a latency-associated peptide (LAP) and a mature TGFB1 peptide (Ref: SWISS-Prot).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Pig,Rat
Immunogen:
A peptide from human and mouse Transforming growth factor beta-1 (372-386 aa).
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
WB and ELISA. Suggested dilution of 1:2,000-1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
TGFB1; TGFB; TGF-beta-1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
TGF beta 1, cross reactivity to other species is expected
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Chicken anti-C-reactive protein (CRP) Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
C-reactive protein has several roles associated with host defence such as; promoting agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. It can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. COFACTOR: Binds 2 calcium ions per subunit. C-reactive protein exists as a homopentamer. There are 2 alternatively spliced isoforms. C-reactive protein is found in plasma and its concentration increases greatly during acute phase response to tissue injury, infection or other inflammatory stimuli. It is induced by IL-1 and IL-6.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Rat
Immunogen:
A peptide from rat C-reactive protein (108-121 aa).
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
WB and ELISA. Suggested dilution of 1:2,000-1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
CRP; PTX1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Rat C-reactive protein
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
FUNCTION: Receptor for the glial cell line-derived neurotrophic factor, artemin. Mediates the artemin-induced autophosphorylation and activation of the RET receptor tyrosine kinase (By similarity). SUBCELLULAR LOCATION: Cell membrane; lipid-anchor; GPI-anchor (By similarity). SIMILARITY: Belongs to the GDNFR family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Mouse,Rat
Immunogen:
A synthetic peptide (MGLSWSPRPPL) as part of mouse GFR alpha-3 protein (aa: 1-11) conjugated to KLH
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB. A dilution of 1:500 to 1:1000 is recommended for both applications. This antibody stains nicely large neurons in the rat DRG. Specifically, if you would like to perform WB using rat brain homogenate detecting GFR alpha-3, this is the antibody to be used. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
GDNF family receptor alpha-3; GFR-alpha-3
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Western blot analysis indicates a high level of specificity to GFR alpha-3 for this antiserum. This antibody is known to react with rat GFR alpha-2.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
FUNCTION: Receptor for neurturin. Mediates the NRTN-induced autophosphorylation and activation of the RET receptor. Also able to mediate GDNF signaling through the RET tyrosine kinase receptor. SUBCELLULAR LOCATION: Cell membrane; lipid-anchor; GPI-anchor (By similarity). ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (PRVEKTPSLPDDLSD) as a part of human GFR alpha-2 protein (aa: 376-390) conjugated to KLH.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB. A dilution of 1:500 to 1:1000 is recommended for both applications. This antibody stains beautifully the large neurons in the rat DRG. This antibody also is ideal for WB analysis of rat DRG and brain homogenate. The optimal dilution should be determined by the end user, but a dilution of 1 in 500 is recommended for initial testing. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Western blot analysis indicates a high level of specificity to GFR alpha-2 for this antiserum. This antibody is known to react with rat GFR alpha-2 and from IHC analysis, there appears to be no cross-reactivity with other members of the GFR family.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Adiponectin Receptors 1 and 2 are membrane receptors for adiponectin, a hormone secreted by adipocytes which regulates energy homeostatis and insulin sensitivity.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. 15% glycerol
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse
Immunogen:
Human Adiponectin Receptor 2 protein (amino acids: 78-219) conjugated to GST.
Applications:
WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting. A dilution of 1:2000 is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Progestin and adipoQ receptor family member II; ADIPOR2; PAQR2;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antiserum is known to recognise both mouse and human Adiponectin Receptor 2.
Storage:
Aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. <br />FUNCTION: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. <br />DISEASE: Defects in GDNF may be a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. DISEASE: Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant human GDNF
Applications:
WB
Antibody Isotype:
Mixed
Application Details:
WB and dot blot. Recommended to be used at a dilution of 1:1000 to 1:3000 for Western blot. The predicted molecular weight of the GDNF monomer is 11.6 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
No cross reactivity with NTN has been observed in Western Blot analysis. This antibody is known to react with human, mouse and rat GDNF.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
APPL1 (adaptor protein containing PH domain, PTB domain and leucine zipper motif 1) mediates adiponectin signaling in various types of cells. APPL1 is also thought to have a role in insulin signaling.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse
Immunogen:
The C-terminus of Human APPL1 protein (amino acids: 455-709) conjugated to GST.
Applications:
ICC,WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting (WB) and Immunoprecipitation (IP). A dilution of 1:1,000 is recommended for WB. A concentration of 3 µL/reaction is recommended for IP. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
DCC-interacting protein 13-alpha; Dip13-alpha; Adapter protein containing PH domain; PTB domain and leucine zipper motif 1; APPL1; APPL; DIP13A; KIAA1428;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antiserum is known to recognise both mouse and human APPL1.
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Purification:
Whole serum
Target:
Adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1)
Rabbit anti-Adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
APPL2 is an isoform of APPL1 with 54% identity in their protein sequences. APPL2 has a role in cell proliferation and embryonic development. Recently, it was shown that APPL2 regulates FSH signaling and acts as a negative regulator in adiponectin signaling.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. 15% glycerol
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse
Immunogen:
Mouse APPL2 protein (amino acids: 619-662) conjugated to GST.
Applications:
ICC,WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting (WB) and Immunoprecipitation (IP). A dilution between 1:2500 and 1:5000 is recommended for WB. A concentration of 3 µL/reaction is recommended for IP. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
DCC-interacting protein 13-beta; Dip13-beta; Adapter protein containing PH domain; PTB domain and leucine zipper motif 2; APPL2; DIP13B;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antiserum is known to recognise both mouse and human APPL2.
Storage:
Aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Purification:
Whole serum
Target:
Adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2)
Rabbit anti-PRKR-like endoplasmic reticulum kinase (phosphorylated and non phosphorylated) (PERK) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
PERK (PKR-like ER kinase) is a single-pass type I ER membrane protein with a stress-sensing luminal domain connected by a transmembrane segment to a cytoplasmic-kinase domain.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. 50% glycerol
Host Animal:
Rabbit
Species Reactivity:
Mouse
Immunogen:
A recombinant peptide from mouse PERK.
Applications:
ICC,WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting (WB) and Immunoprecipitation (IP). A dilution of 1:500 is recommended for WB. A dilution of 30 µL of antibody in a total reaction mixture of 500 µL is recommended for IP. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Feliziani C. et al (2022) Ca2+ signalling system initiated by Endoplasmic reticulum stress stimulates PERK activation Cell Calcium. 2022 [Epub ahead of print] Bollo M. et al (2010) Calcineurin interacts with PERK and dephosphorylates calnexin to relieve ER stress in mammals and frogs PLoS One. 2010 Aug 5;5(8)
Specificity:
This antiserum is known to recognise both phosphorylated and non phosphorylated mouse PERK.
Storage:
Aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Purification:
Whole serum
Target:
PRKR-like endoplasmic reticulum kinase (phosphorylated and non phosphorylated) (PERK)
Chicken anti-Rhodopsin Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
Rod shaped photoreceptor cells that are required for image-forming vision at low light intensity and for photoreceptor cell viability after birth (ref: SWISSPROT).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Purified chicken IgY. Liquid in PBS with 0.02% Sodium Azide. Concentration 1 mg/mL.
Host Animal:
Chicken
Species Reactivity:
Mouse,Rat,Tiger Finch,Zebra Finch
Immunogen:
Mixture of synthetic peptide (14-26 aa) of Mouse Green cones Opsin 1 and synthetic peptide (18-30 aa) of Tiger finch red cones opsin 1 was used as immunogen.
Applications:
WB
Antibody Isotype:
IgY
Application Details:
WB. Suggested dilution at 1:500 to 1:2,000. The antibody recognizes a band of approximately 37 kD on mouse brain lysate. To minimise background staining, a higher concentration of detergent (such as Tween-20) is suggested in the dilution and washing steps. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
Rho; Green cone; Red cone; opsin1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Mouse, Rat, Tiger Finch, Zebra finch
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Chicken anti-Hormone-sensitive lipase (HSL) Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
Hormone Sensitive Lipase (HSL) hydrolyzes stored triglycerides to free fatty acids in adipose tissue and heart. In steroidogenic tissues, HSL principally converts cholesteryl esters to free cholesterol for steroid hormone production (ref: SWISSPROT).
Superoxide dismutase [Mn], mitochondrial destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems (Ref: SwissProt).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized serum with 0.02% thimerosal
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide (KHSLPDLPYDGALEPHINC) of human/rat/mouse mitochondrial manganese Superoxide Dismutase (SOD2), conjugated to Keyhole Limpet Hemocyanin.
Applications:
WB
Antibody Isotype:
Mixed
Application Details:
IF, WB. Typical working dilutions for light microscopy are 1:500 to 1:1,000 depending on tissue and detection method. For IF, a dilution range of 1:50 to 1:100 is recommended. For WB, a dilution range of 1: 1,000 to 1: 4,000 is recommended. This antibody clearly detects a protein at approximately 24 kDa on WB of human brain tissue. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Superoxide dismutase [Mn], mitochondrial;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Mitochondrial manganese Superoxide Dismutase (SOD2), no cross reactivity to SOD1
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles.
FUNCTION: Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. SUBUNIT: Adaptor protein complex 1 (AP-1) is an heterotetramer composed of two large adaptins (gamma1/AP1G1 or gamma2/AP1G2 and beta1A/AP1B1 or beta1B/AP1B1), a medium adaptin (mu1A/AP1M1 or mu1B/AP1M2) and a small adaptin (sigma1A/AP1S1 or sigma1B/AP1S2 or sigma1C/AP1S3). SUBCELLULAR LOCATION: Golgi apparatus. Cytoplasmic vesicle; cytoplasmic vesicle membrane; peripheral membrane protein; cytoplasmic side. Note=Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Widely expressed. DISEASE: Deletion of the AP1B1 gene may play a role in the tumorigenesis of meningiomas. SIMILARITY: Belongs to the adaptor complexes large subunit family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (C-KKGEIFELKAELNSDKKEK) corresponding to the amino acids 11-29 of human AP-1 complex subunit beta-1 conjugated to diphtheria toxin has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from AP-1 complex subunit beta-1 protein in mouse, rat and bovine.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB. This antibody works superbly in immunohistochemistry on frozen or paraffin embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for routine immunohistochemistry are 1: 1000 to 1: 5000 depending on tissue and detection method. For western blotting a dilution range of 1: 100 to 1: 10000 is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Adapter-related protein complex 1 beta-1 subunit; Beta-adaptin 1; Adaptor protein complex AP-1 beta-1 subunit; Golgi adaptor HA1/AP1 adaptin beta subunit; Clathrin assembly protein complex 1 beta large chain; AP1B1; ADTB1; BAM22
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antibody has been shown to be specific for AP-1 complex subunit beta-1. Rat and human, other species have not yet tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
Chicken anti-Hormone-sensitive lipase (HSL) Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
Hormone Sensitive Lipase (HSL) hydrolyzes stored triglycerides to free fatty acids in adipose tissue and heart. In steroidogenic tissues, HSL principally converts cholesteryl esters to free cholesterol for steroid hormone production (ref: SWISSPROT).
WB. Suggested dilution at 1:1,000 to 1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
HSL; LIPE; Hormone-sensitive lipase; Lipe;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antibody detects HSL at approx 83 kDa in various fat cell lysates from mouse and rat. Additional weaker band may appear at approx 40 kDa (unknown). Mouse, Rat, Human
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
SOD1 binds copper and zinc ions ans is one of two isozymes responsible for destroying free superoxide radicals which are normally produced within the cells and which are toxic to biological systems. SOD1 is a soluble cytoplasmic protein, acting as a homodimer to convert superoxide radicals to molecular oxygen and hydrogen peroxide. Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) which is a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
A peptide (HEKADDLGKGGNEESTKTG) corresponding to the amino acids 121-139 of human superoxide dismutase (SOD1) conjugated to diphtheria toxin has been used as the immunogen.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB. This antibody works superbly in immunohistochemistry on frozen or paraffin embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for routine immunohistochemistry are 1: 100 to 1: 1000 depending on tissue and detection method. For western blotting a dilution range of 1: 1000 to 1: 4000 is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Superoxide dismutase; SOD1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antibody has been shown to be specific for superoxide dismutase (SOD1) protein. Human. Does not detect mouse and rat SOD1.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
Rabbit anti-Presenilin 1 loop region Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
Presenilin-1 (PSEN1) is a multi-pass membrane protein and component of the gamma-secretase complex. PSEN1 is thought to play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. It may also play a role in hematopoiesis. Defects in PSEN1 are a cause of Alzheimer disease type 3 (AD3), a familial early-onset form of Alzheimer disease (Ref:SWISS-Prot).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS, pH 7.4. Contains no preservative.
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide (GDPEAQRRVSKNSKYNA-C) corresponding to human PS1 [301-317] in the loop region conjugated via additional C-terminal Cys to Diphtheria toxoid.
Applications:
WB
Antibody Isotype:
IgG
Application Details:
Western Blot. Suggested dilution of 1:1,000 is recommended for WB. Full length presenilin 1 (467 aa) has relative MW of about 45 kDa, with this antibody most commonly detected as cleaved CTF of 19 kDa. Human or mouse brain samples commonly prepared with reducing agent (50mM DTT), urea (2.3 M), SDS (1.5%) in 62.5 mM Tris-HCL pH 6.8 sample buffer (without boiling) heating to 50 C for 15 min. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
Presenilin 1; PS-1; Protein S182; PS1-CTF12; PSEN1; AD3; PS1; PSNL1
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Confirmed by Western blotting using transfected cells, presenilin 1 knock-out mouse cells and mouse and human brain.
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Rabbit anti-Presenilin 2 loop region Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Autosomal dominant mutations in presenilin 2 are the second major cause of early-onset familial Alzheimer's disease. Presenilin 2 is a multi-transmembrane protein which undergoes endoprotelysis to form an N-terminal fragment of about 29 kDa and C-terminal fragment of about 22 kDa. Presenilin 2 forms the catalytic core of the gamma-secretase complex which cleaves type 1 transmembrane proteins including the amyloid precursor protein to generate the C-terminus of the amyloid beta peptide.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS, pH 7.4. Contains no preservative.
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide (KLDPSSQGALQLPYDPEMEEDSYDSFGEP-C) corresponding to human PS1 [306-334] in the loop region conjugated via additional C-terminal Cys to Diphtheria toxoid.
Applications:
ICC,WB
Antibody Isotype:
IgG
Application Details:
WB and IP. Suggested dilution of 1:1,000 is recommended for WB. Full length presenilin 2 (448 aa) has relative MW of about 45 kDa, with this antibody most commonly detected as cleaved CTF of 22 kDa with this antibody. Human or mouse brain samples commonly prepared with reducing agent (50mM DTT), urea (2.3 M), SDS (1.5%) in 62.5 mM Tris-HCL pH 6.8 sample buffer (without boiling) heating to 50 C for 15 min. The suggested dilution for IP is 1:100 . Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
AD3LP, AD5, E5-1, STM-2
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Confirmed by Western blot using mouse and human brain and knock down of presenilin 2 in vitro using siRNA see ref 6 below. Not reactive with presenilin 1.
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Rabbit anti-DJ-1/PARK7 Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
Autosomal recessive mutations in DJ-1 cause early-onset familial Parkinson's disease. DJ-1 is considered a redox-sensitive cytoplasmic protein found in brain as well as other cell types.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS, pH 7.4. Contains no preservative.
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide (ASKRALVILAKGAEE-C) corresponding to human DJ-1 [2-16] in the N-terminal domain conjugated via additional C-terminal Cys to Diphtheria toxoid.
Applications:
WB
Antibody Isotype:
IgG
Application Details:
WB and IHC. Suggested dilution of 1:5,000 is recommended for WB. DJ-1 is a soluble protein of 189 amino acids and detected with 23 kDa mobility by western blotting. The suggested dilution for IHC is 1:100. Detected astrocyte cytoplasmic labelling in human brain formaldehyde-treated tissue. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
PARK7
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Confirmed by WB using soluble mouse and human brain extracts, reactivity for major product diminished by peptide absorption.
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Chicken anti-Adiponectin Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
Adiponectin is synthesized by adipocytes and is involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities (ref: SWISSPROT).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide from human Adiponectin (230-244 aa).
Applications:
WB
Antibody Isotype:
IgY
Application Details:
WB. Suggested dilution of 1:2,000-1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Guinea Pig anti-Agouti related protein (AGRP) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
AGRP is the endogenous antagonist of alpha-melanocyte stimulating hormone and has been shown to cause potent stimulation of food intake, and this protein is found in over 90% of Neuropeptide Y containing cells in rats.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Guinea Pig
Species Reactivity:
Human,Mouse,Rat,Sheep
Immunogen:
A synthetic peptide (SPRRCVRLHESCLGQQVPCCDPCATCYCRFFNAFCYCRKLGTATNLCSRT) corresponding to a region (82-131) within the carboxy domain of mouse agouti related protein. This peptide was conjugated to carrier protein to enhance the immunological response.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC, frozen, PFA fixed material. Not yet tested on paraffin embedded tissue sections. A concentration of 1:1000 to 1:2000 is recommended for IHC with overnight incubations. Permeabilization suggested is 0.1% triton X-100 in blocking buffer. IHC performed in sheep brain (hypothalamus) demonstrates intense staining of cells and terminals. No staining is evident when the primary antibody is pre-absorbed with 0.5 mg/mL of AGRP. In rodents such as rat cell terminal staining is most typically observed without cell body staining. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Agrp; Agrt; Art; Agouti-related protein;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specificity was demonstrated by immunohistochemistry. This antibody is known to react with human, rat and sheep. Other species have not yet been tested.
Storage:
Store lyophilized antibody at 2-8ºC. It is recommended that a thawed sample is stored at 2-8°C for no longer than 2 weeks. Allocation of appropriate anti-bacterial agent can increase shelf life by several weeks. Diluted serum should be prepared as required. Long term stability requires storage, preferably in small aliquots at -20°C or lower. Glycerol (1:1) can be added to neat serum for additional stability if intended use does not prevent this.
Rabbit anti-Met-Enkephalin Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen, IHC-Paraffin-embedded, ICC.
Background Info:
Human Methionine enkephalin (Met-Enkephalin) is a small 5 amino acid peptide cleaved from the precursor pro-opiomelanocortin (POMC). Met-Enkephalin is an endogenous opioid peptide that interacts with opioid receptors and produces analgesic effects.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized with BSA
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Synthetic human Met-enkephalin peptide (237-241) conjugated to BSA.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded
Antibody Isotype:
IgG
Application Details:
A dilution of 5-10 µg/mL is recommended for immunohistochemistry using formalin fixed and paraffin embedded tissues and for 4% paraformaldehyde fixed frozen tissues. A dilution of 5-15 µg/mL is recommended for immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Pro-opiomelanocortin; POMC;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human; mouse; rat. Met-Enkephalin is highly conserved so cross-reactivity with other species is expected. Cross-reactivity with other opioid peptides is as follows: with Leu-enkephalin 5.8%; with beta-lipotropin 0.01%; with beta-endorphin 0.01%
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 2-8°C with an appropriate antibacterial agent.Avoid freeze-thaw cycles
Rabbit anti-Leu-Enkephalin Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen, IHC-Paraffin-embedded, ICC.
Background Info:
Leu-Enkephalin is cleaved from the precursor Proenkephalin-A. Leu-Enkephalin is an endogenous opioid peptide that interacts with opioid receptors and produces analgesic effects.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized with BSA
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Synthetic human Leu-Enkephalin peptide (230 - 234) conjugated to BSA.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded
Antibody Isotype:
IgG
Application Details:
A dilution of 5-10 µg/mL is recommended for immunohistochemistry using formalin fixed and paraffin embedded tissues and for 4% paraformaldehyde fixed frozen tissues. A dilution of 5-15 µg/mL is recommended for immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Proenkephalin-A; PENK;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human; mouse; rat. Leu-Enkephalin is highly conserved so cross-reactivity with other species is expected. Cross-reactivity with other opioid peptides is as follows: with Met-enkephalin 0.93%; with beta-lipotropin 0.01%; with beta-endorphin 0.01%
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 2-8°C with an appropriate antibacterial agent.Avoid freeze-thaw cycles
Chicken anti-Neurotrophin-3 (NT-3) Polyclonal Antibody (Unconjugated), suitable for ELISA.
Background Info:
FUNCTION: Seems to promote the survival of visceral and proprioceptive sensory neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse
Immunogen:
Mixture of two human NT3 peptides (90-104 and 199-214 aa).
Applications:
ELISA
Antibody Isotype:
IgY
Application Details:
ELISA at suggested dilution of 1:500-1:2,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Mouse anti-Tyrosine hydroxylase (TH) Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded, ICC, IP, FC.
Background Info:
Tyrosine hydroxylase is an excellent marker for dopaminergic and noradrenergic neurons. Tyrosine hydroxylase (a.k.a. tyrosine 3-monooxygenase) is the enzyme responsible for catalyzing the conversion of the amino acid L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA). L-DOPA is a precursor for dopamine, which, in turn, is a precursor for the important neurotransmitters norepinephrine (noradrenaline) and epinephrine (adrenaline). Tyrosine hydroxylase catalyzes the rate limiting step in this synthesis of catecholamines. In humans, tyrosine hydroxylase is encoded by the TH gene, and the enzyme is present in the central nervous system (CNS), peripheral symphatic neurons and the adrenal medulla. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing. Tyrosine hydroxylase, phenylalanine hydroxylase and tryptophan hydroxylase together make up the family of aromatic amino acid hydroxylases (AAAHs).
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized, dry powder.
Host Animal:
Mouse
Species Reactivity:
Chicken,Frog,Horse,Human,Mouse,Primate,Rat,Sheep,Vole,Zebra Fish
Immunogen:
Tyrosine Hydroxylase purified from PC12 cells
Applications:
FC,ICC,IHC-Frozen,IHC-Paraffin-embedded,IP,WB
Clone number:
LNC, LNC1, LNC-1
Antibody Isotype:
IgG1, kappa
Application Details:
Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry/paraffin embedded IH(P), Immunoprecipitation (IP), Immunofluorescence (IF), Flow cytometry (FC).WB: 1:1000 -1:2000, SDS reduced samples. Detects a 59-61kDa protein. Rat Brain lysates is a suitable control. IHC/IH(P): Reacts in formalin fixed paraffin embedded tissues with HIER antigen recovery. Typical dilution is 1:100-1:200 depending upon incubation time and detection method used. IF: 1:200-1:1000, 4% PFA fixed tissues/cells permeabilized with 0.1-0.4% triton X-100; also works in fresh frozen and acetone fixed tissues/cells.IP: 1:100, immobilized on protein A beads, Fleming-Jones et al (1995) J. Protein Chemistry 14(5):275-282.FC: Fixed, permeabilized dopaminergic nerve terminals from rat striatum, {Wolf, ME, Kapatos, G (1989) The Journal of Neuroscience, January 1989, 9(l): 108-114; Wolf ME, Zigmond, MJ, Kapatos, G (1989) J. Neurochemistry 53(3):879-885}.
Alternative Names:
LNC-1; LNC1; TH monoclonal
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Kapatos G., Kemski V., and Geddes T. (1989) Dopamine neurons in monolayer culture as a model system for the study of tyrosine hydroxylase, in Pteridines and Biogenic Amines in Neuropsychiatry, Pediatrics and Immunology (Levine R. A., KuhnD. M., Milstien S., and Curtius H-C., eds), pp. 243-258. Lakeshore Publishers, Grosse Pointe, Michigan.
Specificity:
Clone LNC 1 recognizes an epitope on the outside of the regulatory N-terminus. The clone detects a protein of approximately 59-61 kDa by Western blot and reduced SDS-PAGE. The clone does not react with dopamine-beta-hydroxylase, phenylalanine hydroxylase, trytophan hydroxylase, dehydropteridine reductase, sepiapterin reductase or phenethanolamine-N-methyl transferase (PNMT) by western blots. Chicken, Frog, Horse, Human, Monkey, Mouse, Vole, Sheep, Zebrafish other species not yet tested
Storage:
After reconstitution keep aliquots at -20 ° to -70°C for a higher stability. At 2-8°C keep up to one week, insulated, protected from light; use sterile methods and pipettes. Highly purified glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles. Keep tightly closed when not in use and protected from light
Purification:
Unpurified ascites fluid, diluted with PBS containing 3% BSA, no preservatives.
Rabbit anti-Gastrin Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen, IHC-Paraffin-embedded, ICC.
Background Info:
Human Gastrin is a 101 amino acid hormone produced by G cells of the duodenum, stomach and pancreas. It stimulates secretion of hydrochloric acid by parietal cells of the stomach. Gastrin is also secreted into bloodstream.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized with BSA
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Synthetic human Gastrin peptide (76-85 aa) conjugated to BSA.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded
Antibody Isotype:
IgG
Application Details:
A dilution of 5-10 µg/mL is recommended for immunohistochemistry using formalin fixed and paraffin embedded tissues and for 4% paraformaldehyde fixed frozen tissues. A dilution of 5-15 µg/mL is recommended for immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Human Gastrin , Cross reactivity is expected with mouse, rat, other species not yet tested.
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 2-8°C with an appropriate antibacterial agent.Avoid freeze-thaw cycles
Endomorphins 1 and 2 are endogenous opioid peptides which have the highest affinity for the mu-opioid receptors. Located in various parts of the brain and interacts with mu-opioid receptors and produces analgesia.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized with BSA
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Synthetic Endomorphin-2 (YPFF) peptide conjugated to BSA. Selectivity towards Endomorphin-2 was achieved by separating fraction of affinity purified antibodies which did not bind to Endomorphin-1.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded
Antibody Isotype:
IgG
Application Details:
A dilution of 5-10 µg/mL is recommended for immunohistochemistry using formalin fixed and paraffin embedded tissues and for 4% paraformaldehyde fixed frozen tissues. A dilution of 5-15 µg/mL is recommended for immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
EM 2;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Endomorphin-2 peptide
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 2-8°C with an appropriate antibacterial agent.Avoid freeze-thaw cycles
Rabbit anti-Endomorphin-1 and Endomorphin-2 (E-1/E-2) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen, IHC-Paraffin-embedded, ICC.
Background Info:
Endomorphins 1 and 2 are endogenous opioid peptides which have the highest affinity for the mu-opioid receptors. Located in various parts of the brain and interacts with mu-opioid receptors and produces analgesia.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized with BSA
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Antibodies to human Endomorphins 1 and 2 were made by immunising rabbits with the human Endomorphin-1 peptide (YPWF) and then selecting those antibodies that cross-reacted with both human Endomorphins 1 and 2.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded
Antibody Isotype:
IgG
Application Details:
A dilution of 5-10 µg/mL is recommended for immunohistochemistry using formalin fixed and paraffin embedded tissues and for 4% paraformaldehyde fixed frozen tissues. A dilution of 5-15 µg/mL is recommended for immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
EM 1; EM 2;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human; mouse; rat. Endomorphin-1 is highly conserved molecule so cross-reactivity with other species is expected. Cross-reactivity with other opioid peptides is as follows: with Endomorphin-2 = 70%; Met-enkephalin <0.03%; with Leu-enkephalin 0.01%; with beta-endorphin<0.1%.
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 2-8°C with an appropriate antibacterial agent.Avoid freeze-thaw cycles
The amyloid beta peptide is derived from the cleavage of the Amyloid precursor protein (APP) and varies in length from 39 to 43 amino acids. However, the form(s) of amyloid-beta peptide (A? associated with the pathology characteristic of Alzheimer's disease (AD) remains unclear. In particular, the neurotoxicity of intraneuronal A? accumulation is an area of considerable research and controversy principally because antibodies thought to be specific for A? have been shown to actually detect intraneuronal APP and not A? exclusively.<br /><br />MOAB-2 (mouse IgG2b) is a pan-specific, high-titer antibody to A? residues 1-4 as demonstrated by biochemical and immunohistochemical analyses (IHC), and is highly specific just to amyloid beta peptide.<br /><br />MOAB-2 did not detect APP or APP-CTFs in cell culture media/lysates (HEK-APPSwe or HEK APPSwe/BACE1) or in brain homogenates from transgenic mice expressing 5 familial AD (FAD) mutation (5xFAD mice). <br /><br />Using IHC on 5xFAD brain tissue, MOAB-2 immunoreactivity co-localized with C-terminal antibodies specific for A?40 and A?42. MOAB-2 did not co-localize with either N- or C-terminal antibodies to APP. In addition, no MOAB-2-immunreactivity was observed in the brains of 5xFAD/BACE-/- mice, although significant amounts of APP were detected by N- and C-terminal antibodies to APP, as well as by 6E10.<br /><br />In both 5xFAD and 3xTg mouse brain tissue, MOAB-2 co-localized with cathepsin-D, a marker for acidic organelles, further evidence for intraneuronal A?, distinct from A? associated with the cell membrane. MOAB-2 demonstrated strong intraneuronal and extra-cellular immunoreactivity in 5xFAD and 3xTg mouse brain tissues.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized, from a Protein A purified preparation in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, 0.01% sodium azide, 0.1% trehalose, pH 7.2; contains 0.01% sodium azide as a preservative.
Host Animal:
Mouse
Species Reactivity:
Human,Rat
Immunogen:
Recombinant human amyloid beta protein 42 (A?42): DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA
Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry/paraffin embedded IH(P), Immunoprecipitation (IP), Immunofluorescence (IF), ELISA.<br><br>Antibody has been tested in WB using purified synthetic beta-amyloid preparations and from transgenic mouse brain formic acid extracts (see figure 1). Formic acid extraction/concentration is required for western blot detection from extracts. MOAB-2 antibody is specific for beta-amyloid and does not detect APP. Suggested dilution of 1:2000-1:5,000 for WB, standard ECL detection systems. <br><br>Tissue samples for the detection of beta-amyloid should be prepared as detailed in K.L. Youmans et al. {Journal of Neuroscience Methods 196 (2011) 51-59} for best results. Detection of beta-amyloid 40/42 in direct westerns can be difficult; Dot-blots of prepared samples are recommended as detailed in Youmans. KL et al 2012. <br><br>IR or fluorescent detection systems not yet tested, they but are expected to work well with higher primary antibody dilutions because of the increased sensitivity of the detection methods.<br><br>Suggested dilutions for IHC are 1:50-1:1,000. Fresh frozen, 4% paraformaldehyde fixed frozen, or formalin fixed paraffin embedded tissues are all suitable. Optimal dilutions must be determined by the end user. Antigen retrieval is required in fixed tissues for optimal staining.<br><br>Antibody was tested on 4% paraformaldehyde/0.1% glutaraldehyde fixed frozen tissue from 3xTg and 5xFAD mice. MOAB-2 antibody detects intraneuronal and extracellular beta-amyloid in IHC and does not detect APP {Youmans KL et al 2012}.<br><br> The antibody also reacts with archival formalin-fixed, paraffin-embedded tissue samples with antigen Heat Induced Epitope Retrieval (HIER): Recommended Citrate, pH 6.0 buffer for HIER. Signal was weak without antigen retrieval. Immunoreactively was expressed in intraneural-amyloid deposition (plaque) in Alzheimer's brain. MoAB-2 was found to be extremely clean and with an excellent signal to noise ratio with no neuro-cellular diffusive staining.<br><br>In addition MOAB-2 demonstrated no significant differences in A-beta detection using paraffin fixed, free-floating sections {Youmans KL et al 2012}. Formic acid (FA) treatment resulted in optimal detection of both intraneuronal and extracellular A-beta compared to without FA (incubated in 88% FA 8 min, Youmans KL et al 2012). Free floating tissue sections were permeabilized in TBS containing 0.25% Triton X-100 (TBSX; 3 x 10 min), blocked with 3% horse serum in TBSX (3 x 10 min) followed by 1% horse serum in TBSX (2 x10 min) and incubated with appropriate primary antibodies diluted in TBSX containing 1% horse serum overnight. See Youmans KL et al 2012 for full IH(P) protocol and method details.<br><br> For IF, suggested dilution is 1:100-1:500. The antibody was tested on 4% PFA fixed frozen tissue. Fixed tissues were washed in TBS (3 x 10 min), then incubated in 88% FA (8 min), and then permeabilized in TBSX (3 x 10 min), and blocked in TBSX containing 5% bovine serum albumin (BSA; 1 hr). Sections were subsequently incubated with appropriate primary antibodies diluted in TBSX containing 2% BSA overnight on an oscillatory rotator. Detection was via fluorescently labelled absorbed secondary antibodies {Youmans KL et al 2012}.<br><br>For IP, the suggested dilution is 1:200 to 1:1,000 for labeled beta-amyloid using Protein A/G conjugated beads as the capture vehicle {Youmans KL et al 2012}.<br><br>In an ELISA, a dilution of 1:50-1:1000 is suggested. The antibody has been tested in ELISAs on synthetic beta-amyloid and tissue homogenates from beta-amyloid-Tg mice. Biosensis recommends optimal dilutions/concentrations should be determined by the end user for all applications. Dilutions provided are only meant to serve as a basic guide.
Alternative Names:
Beta-APP42; Beta-APP40; Beta-amyloid protein 42; Beta-amyloid protein 40; ABPP; APPI; Amyloid beta A4 protein;MOAB2;MOAB-2; Alzheimer's antibody;AB40;AB42;abeta
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Setti, S.E. et al. (2022) Assessment of sex-related neuropathology and cognitive deficits in the Tg-SwDI mouse model of Alzheimers disease. Behave Brain Res. 428:113882. Application: IHC. Sil, A. et al. (2022) Sex Differences in Behavior and Molecular Pathology in the 5XFAD Model. J Alzheimers Dis. 85(2):755-778. Application: WB. Sarkar, S. et al. (2020) Modification of methods to use Congo-red stain to simultaneously visualize amyloid plaques and tangles in human and rodent brain tissue sections. Metab Brain Dis. [Epub ahead of print]. Application: IHC. Cuevas, E. et al. (2019) Amyloid Beta 25-35 induces blood-brain barrier disruption in vitro. Metab Brain Dis. [Epub ahead of print]. Application: ICC/IF. Schmued, L. et al. (2019) High Contrast and Resolution Labeling of Amyloid Plaques in Tissue Sections from APP-PS1 Mice and Humans with Alzheimer's Disease with the Zinc Chelator HQ-O: Practical and Theoretical Considerations. Curr Alzheimer Res. 16(7):577-586. Application: IHC/IF. Hui, L. et al. (2019) Acidifying Endolysosomes Prevented Low-Density Lipoprotein-Induced Amyloidogenesis. J Alzheimers Dis. 64(1):393-410. Application: ICC/IF. Koss, DJ. et al. (2018) Distinctive temporal profiles of detergent-soluble and -insoluble tau and A? species in human Alzheimer's disease. Brain Res. [Epub ahead of print]. Application: WB, dot blot. Zhao, Y. et al. (2018) TREM2 Is a Receptor for _-Amyloid that Mediates Microglial Function. Neuron. 97(5):1023-1031. Application: IHC, free-floating cryostat sections Zhu, B. et al. (2017) ER-associated degradation regulates Alzheimer's amyloid pathology and memory function by modulating _-secretase activity. Nat Commun. 8(1):1472. Application: IHC Huang, TY. et al. (2017) SORLA attenuates EphA4 signaling and amyloid _-induced neurodegeneration. J Exp Med. pii: jem.20171413. [Epub ahead of print]. Application: IHC Felecia, M. et al. (2017) Peripheral Inflammation, Apolipoprotein E4, and Amyloid-_ Interact to Induce Cognitive and Cerebrovascular Dysfunction. ASN Neuro. 9(4):1759091417719201. Application: IHC/IF Thomas, R. et al. (2016) Epidermal growth factor prevents APOE4 and amyloid-beta-induced cognitive and cerebrovascular deficits in female mice. Acta Neuropathol Commun. 4(1):111 Application: IHC Koster, KP. et al. (2016) Epidermal growth factor prevents oligomeric amyloid-_ induced angiogenesis deficits in vitro. J Cereb Blood Flow Metab. [Epub ahead of print] Application: IF Loffler, T. et al. (2016) Decreased Plasma A? in Hyperlipidemic APPSL Transgenic Mice Is Associated with BBB Dysfunction. Front. Neurosci. Application: IF Kobro-Flatmoen, A. et al. (2016) Reelin-immunoreactive neurons in entorhinal cortex layer II selectively express intracellular amyloid in early Alzheimer's disease. Neurobiology of Disease. 93:172-183. Application: IHC Tai, LM. et al. (2016) The role of APOE in cerebrovascular dysfunction. Acta Neuropathol. 131(5):709-23. Application: IF Kim, YH. et al. (2015) A 3D human neural cell culture system for modeling Alzheimer's disease. Nat Prot. 10(7):985-1006. Application: WB Condello, C. et al. (2015) Microglia constitute a barrier that prevents neurotoxic protofibrillar A?42 hotspots around plaques. Nat Commun. 6:6176. Application: IF Iulita MF et al (2014) Studying Alzheimer's Disease Pre-clinical Stages: Insights from Down's Syndrome and Transgenic Animal Models. PhD Thesis Application: IHC/IF Iulita MF et al (2014) Intracellular Abeta pathology and early cognitive impairments in a transgenic rat model overexpressing human amyloid precursor protein: a multidimensional study. Acta Neuropathol Commun. 6:61. Application: IF, IH Smith BR et al (2014) Neuronal inclusions of alpha-synuclein contribute to the pathogenesis of Krabbe disease. J Pathol. Apr;235(5):509-21. Application: IF
Specificity:
MOAB-2 detects preparations enriched in U-, O-, F-A?42, and U-A?40 by dot-blot, and is thus a pan-specific A? antibody. However, MOAB-2 is selective for the more neurotoxic A?42 compared to A?40. Indeed, MOAB-2 demonstrated a titration against antigen concentration, and detects A?40 at 2.5 pmol but U-, O- and FA?b42 at antigen concentrations as low as ~ 0.1 pmol {Youmans. KL et al 2012}. MOAB-2 does not detect APP (Amyloid precursor protein). Human, Rat, other species not yet tested.By Dot blot, MOAB-2 detected rat A?40 and human A?40, albeit with less affinity than for A?42. {Youmans. KL et al 2012}
Storage:
After reconstitution keep aliquots at -20 ° to -70°C for a higher stability. At 2-8°C keep up to one week, insulated, protected from light; use sterile methods and pipettes. Highly purified glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles. Keep tightly closed when not in use and protected from light.
Purification:
This product is a Protein A purified mouse IgG2b in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, 0.01% sodium azide, pH 7.2.
Glutathione peroxidase 1 has a role in detoxification of hydrogen peroxide and is one of the most important antioxidant enzymes in humans. It exists as a homotetramer which localises to the cytoplasm. It belongs to the glutathione peroxidase family. Glutathione peroxidase 1 is one of few proteins in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by UGA, that normally functions as a translation termination codon. This protein has a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine repeats. The allele with five alanine repeats is significantly associated with breast cancer risk. At least two alternatively spliced isoforms have been identified.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized serum with 0.02% thimerosal
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (RRYSRRFQTIDIEPDIEALL) corresponding to the amino acids 175-194 of human glutathione peroxidase 1 ( GPx-1) conjugated to diphtheria toxin has been used as the immunogen.
Applications:
ELISA,IHC-Paraffin-embedded,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB and ELISA. This antibody works superbly in IHC on frozen or wax embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for routine IHC are 1: 200 to 1: 1,000 depending on tissue and detection method. For WB a dilution range of 1: 1,000 to 1: 4,000 is recommended. For ELISA, a dilution of 1: 1,000 to 1: 4,000 is recommended. This antiserum has extremely high titre, it stains human and rat glial cells intensely and to some extent also stains neurons. Other tissues have not yet been tested. On WB under reducing conditions recognises a 22kD protein in human brain tissue and Sigma glutathione peroxidase (G-4013) isolated from human erythrocytes. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Human and rat cellular selenium dependent glutathione peroxidase. Human; rat;
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles.
Glutathione peroxidase 4 (GPx-4) is involved in protecting cells against membrane lipid peroxidation and cell death.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized serum with 0.02% thimerosal
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (VNYTQLVDLHARYAEC) corresponding to the amino acids 78-93 of human glutathione peroxidase 4 (Isoform Mitochondrial) conjugated to KLH has been used as the immunogen. Human, rat and mouse sequences are identical.
Applications:
IHC-Paraffin-embedded,WB
Antibody Isotype:
Mixed
Application Details:
WB, IHC. Typical working dilution for IHC is 1:200 to 1:1,000 depending on tissue and detection method. For WB, a dilution range of 1:1,000 to 1:2,000 is recommended. GPx-4 exists as a tetramer and can reform into multimeric complexes even under reduced conditions. The reported molecular weight of the GPx-4 monomer is 22 kDa but is reported to rapidly form oligomers, thus higher molecular weight bands greater than 22 kDa are expected. Mitochondrial preparations are also recommended to enhance signal. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Human and rat mitochondrial glutathione peroxidase (GPx-4) Human; rat;
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles.
Peroxiredoxin-1 has a role in redox regulation of the cell.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized serum with 0.02% thimerosal
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (PLVSDPKRTIAQDY) corresponding to the region 103-116 amino acids of human Peroxiredoxin-1 conjugated to diptheria toxin.
Applications:
ELISA,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB, ELISA. This antibody works in IHC on frozen or wax embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for light microscopy are 1:500 to 1:1,000 and IF of 1:50 to 1:100. For WB a dilution range of 1: 1,000 to 1: 4,000 is recommended. A dilution of 1: 1,000 to 1: 4,000 is recommended for ELISA. This antibody stains non-ciliated bronchiolar cells in rat airways and specific kidney tubule cells. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles.
Adiponectin Receptors 1 and 2 are membrane receptors for adiponectin, a hormone secreted by adipocytes which regulates energy homeostatis and insulin sensitivity.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. 15% glycerol
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse
Immunogen:
The intracellular portion of mouse Adiponectin Receptor 1 protein (amino acids: 4-142) conjugated to GST.
Applications:
WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting. A dilution of 1:2000 is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Adipor1; AdipoR1; Progestin and adipoQ receptor family member I; ADIPOR1; PAQR1;TESBP1A; CGI-45;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antiserum is known to recognise both mouse and human Adiponectin Receptor 1.
Storage:
Aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Goat anti-C-reactive protein (CRP) Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. COFACTOR: Binds 2 calcium ions per subunit.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Goat
Species Reactivity:
Human
Immunogen:
Human recombinant C-Reactive protein
Applications:
WB
Antibody Isotype:
Mixed
Application Details:
WB. Extremely high titre antibody. For western blot a dilution of 1: 10,000 to 1: 100000 is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
CRP; PTX1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
It has been shown to be specific for C-reactive protein by WB. Human
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent.
Mouse anti-Lipid phosphate phosphohydrolase 3 (LPP3) Monoclonal Antibody (Unconjugated), suitable for WB, FC.
Background Info:
Lipid phosphate phosphohydrolase 3 (LPP3) is a member of the phosphatidic acid phosphatase (PAP) family. LPP3 catalyzes the conversion of phosphatidic acid to diacylglycerol. In addition it hydrolyzes lysophosphatidic acid, ceramide-1-phosphate and sphingosine-1-phosphate (Ref: SWISSPROT).
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS pH 7.4
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
A synthetic peptide from human LPP3 (179-196 aa) conjugated to KLH.
Applications:
FC,WB
Clone number:
7H7D3
Antibody Isotype:
Mix of IgG1, IgG2a & IgG2b
Application Details:
Western Blotting (WB), Flow cytometry (FACS) and Immunohistochemistry (IHC). For WB, the recommended concentration is 2-3 µg/mL. For IHC, this antibody has been shown to work on formalin-fixed, paraffin-embedded tissue samples with heat-induced antigen retrieval. The recommended concentration is 0.5-2 µg/mL. For FACS, the recommended concentration is 2.0 µg/mL. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Lipid phosphate phosphohydrolase 3; PAP2-beta; Phosphatidate phosphohydrolase type 2b; Phosphatidic acid phosphatase 2b; PAP-2b; PAP2b; Vascular endothelial growth factor and type I collagen-inducible protein; VCIP; PPAP2B;LPP3
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Mueller PA (2016) PPAP2B expression limits lesion formation in murine models of atherosclerosis. Doctoral Dissertation. Species: Mouse. Application: IHC. Reschen ME, Gaulton KJ, Lin D, Soilleux EJ, Morris AJ, Smyth SS and O'Callaghan CA (2015) Lipid-induced epigenomic changes in human macrophages identify a coronary artery disease-associated variant that regulates PPAP2B Expression through Altered C/EBP-beta binding. PLoS Genet. 2015 Apr 2;11(4):e1005061. Species: Human. Application: IHC with heat-induced antigen retrieval. Humtsoe JO, Liu M, Malik AB and Wary KK (2010) Lipid phosphate phosphatase 3 stabilization of beta-catenin induces endothelial cell migration and formation of branching point structures. Mol Cell Biol. Apr;30(7):1593-606. Species: Human. Application: WB and IHC.
Specificity:
Confirmed by over-expression of human LPP3 cDNA. Human
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 2-8°C with an appropriate antibacterial agent. Avoid freeze-thaw cycles.
Peroxiredoxin-2 has a role in redox regulation of the cell.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized serum with 0.02% thimerosal
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (VVDGAFKEVKLS) corresponding to region (20-31 aa) from human Peroxiredoxin-2 conjugated to diptheria toxin.
Applications:
ELISA,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB, ELISA. This antibody works in IHC on frozen or wax embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for light microscopy are 1:500 to 1:1,000 and IF of 1:50 to 1:100. For WB a dilution range of 1: 1,000 to 1: 4,000 is recommended. A dilution of 1: 1,000 to 1: 4,000 is recommended for ELISA. This antibody stains basal cells in rat airways and specific kidney tubule cells. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Human Peroxiredoxin-2, no cross reactivity to other family members
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles.
Peroxiredoxin-3 has a role in redox regulation of the cell.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized serum with 0.02% thimerosal
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (CEVVAVSVDSHFSHLAW) from a region (127-143 aa) on human Peroxiredoxin-3 conjugated to KLH.
Applications:
ELISA,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB, ELISA. This antibody works in IHC on frozen or wax embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for light microscopy are 1:500 to 1:1,000 and IF of 1:50 to 1:100. For WB a dilution range of 1: 1,000 to 1: 2,000 is recommended. This antibody detects a protein at approx 23 kDa on WB of human brain tissue which is the mature form of this protein. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Thioredoxin-dependent peroxide reductase, mitochondrial; Antioxidant protein 1; AOP-1; HBC189; Peroxiredoxin III; Prx-III; Peroxiredoxin-3; Protein MER5 homolog; PRDX3; AOP1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human Peroxiredoxin-3 no cross reactivity to other family members
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles.
Peroxiredoxin-4 has a probable role in redox regulation of the cell.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (SRVSVADHSLHLSKAKISK) corresponding to a region (65-83 aa) from human Peroxiredoxin-4 conjugated to diptheria toxin.
Applications:
ELISA,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB, ELISA. This antibody works in IHC on frozen or wax embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for light microscopy are 1:500 to 1:1,000 and IF of 1:50 to 1:100. For WB a dilution range of 1: 1,000 to 1: 4,000 is recommended. A dilution of 1: 1,000 to 1: 4,000 is recommended for ELISA. This antibody stains non-ciliated bronchiolar cells in rat lung and what appears to be sebaceous glands in rat skin. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Tam KC et al. (2018) Evidence for the interaction of peroxiredoxin-4 with the store-operated calcium channel activator STIM1 in liver cells. Cell Calcium. 2018 May 15;74:14-28.
Specificity:
Human Peroxiredoxin-4 no cross reactivity to other family members
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles.
Endomorphins 1 and 2 are endogenous opioid peptides which have the highest affinity for the mu-opioid receptors. Located in various parts of the brain and interacts with mu-opioid receptors and produces analgesia.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized with BSA
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Synthetic human Endomorphin-1 (YPWF) peptide conjugated to BSA. Selectivity towards Endomorphin-1 was achieved by separating fraction of affinity purified antibodies which did not bind to Endomorphin-2.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded
Antibody Isotype:
IgG
Application Details:
A dilution of 5-10 µg/mL is recommended for immunohistochemistry using formalin fixed and paraffin embedded tissues and for 4% paraformaldehyde fixed frozen tissues. A dilution of 5-15 µg/mL is recommended for immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
EM 1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human; mouse; rat. Endomorphin-1 is highly conserved so cross-reactivity with other species is expected. Cross-reactivity with other opioid peptides is as follows: with Endomorphin-2 <5%; Met-enkephalin <0.03%; with Leu-enkephalin 0.01%; with beta-endorphin<0.1%
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 2-8°C with an appropriate antibacterial agent.Avoid freeze-thaw cycles
Human beta-Lipotropin is a 93 amino acid polypeptide that is cleaved from carboxy-terminal fragment of the precursor pro-opiomelanocortin (POMC). It stimulates melanocytes to produce melanin, and can also be cleaved into smaller peptides including opioid peptides: gamma-lipotropin, alpha-MSH, beta-MSH, gamma-MSH, alpha-endorphin, beta-endorphin, gamma-endorphin and met-enkephalin
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized with BSA
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Synthetic peptide (179-267 aa) of human beta-Lipotropin conjugated to thyroglobulin was used as the immunogen.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded
Antibody Isotype:
IgG
Application Details:
A dilution of 5-10 µg/mL is recommended for immunohistochemistry using formalin fixed and paraffin embedded tissues and for 4% paraformaldehyde fixed frozen tissues. A dilution of 5-15 µg/mL is recommended for immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Human; mouse; rat. Beta-Lipotropin is highly conserved so cross-reactivity with other species is expected. Cross-reactivity with other opioid peptides is as follows: with Leu-enkephalin 0.01%; with Met-Enkephalin 0.01%; with beta-endorphin 0.01%
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 2-8°C with an appropriate antibacterial agent.Avoid freeze-thaw cycles
Peroxiredoxin-5 has a role in intracellular redox signaling.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized serum with 0.02% thimerosal
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (VKALNVEPDGTGLTC) corresponding to region (190-204 aa) of human Peroxiredoxin-5 conjugated to KLH. Human, mouse and rat sequences of this peptide are identical.
Applications:
ELISA,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
Mixed
Application Details:
IHC, IF, WB. This antibody works in IHC on frozen or wax embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for light microscopy are 1:500 to 1:1,000 and IF 1:50 to 1:100. For WB a dilution range of 1: 1,000 to 1: 2,000 is recommended. This antibody clearly detects a protein at approximately 22 kDa on WB of human brain tissue. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Human Peroxiredoxin-5 no cross reactivity to other family members
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles.
Beta-adrenergic receptors are multi-pass membrane proteins that belong to the G-protein coupled receptor 1 family. Their function is to mediate the catecholamine-induced activation of adenylate cyclase through the action of G-proteins (ref: SWISSPROT).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Mouse,Rat
Immunogen:
A synthetic peptide from mouse beta 3 Adrenergic Receptor (386-400 aa).
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
WB and ELISA. Suggested dilution of 1:2,000-1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Rabbit anti-Gamma-synuclein Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
FUNCTION: Plays a role in neurofilament network integrity. May be involved in modulating axonal architecture during development and in the adult. In vitro, increases the susceptibility of neurofilament-H to calcium-dependent proteases. May also function in modulating the keratin network in skin. Activates the MAPK and Elk-1 signal transduction pathway. SUBUNIT: May be a centrosome-associated protein. SUBCELLULAR LOCATION: Cytoplasm; perinuclear region. Centrosome. Spindle. Associated with centrosomes in several interphase cells. In mitotic cells, localized to the poles of the spindle. TISSUE SPECIFICITY: Highly expressed in brain, particularly in the substantia nigra. Also expressed in the corpus callosum, heart, skeletal muscle, ovary, testis, colon and spleen. Weak expression in pancreas, kidney and lung. PTM: Phosphorylated. Phosphorylation by GRK5 appears to occur on residues distinct from the residue phosphorylated by other kinases. DISEASE: Brain iron accumulation type 1 (NBIA1, also called Hallervorden-Spatz syndrome), a rare neuroaxonal dystrophy, is histologically characterized by axonal spheroids, iron deposition, Lewy body (LB)-like intraneuronal inclusions, glial inclusions and neurofibrillary tangles. SNCG is found in spheroids but not in inclusions. SIMILARITY: Belongs to the synuclein family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (EKEEVAEEAQSGGD) as part of human gamma synuclein protein (aa: 114-127) conjugated to diptheria toxid has been used as the immunogen.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB. A dilution of 1:500 to 1:1000 is recommended for both applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Immunohistochemical/western blot analysis indicate a high level of specificity for this antiserum for gamma synuclein. This antiserum is known to react with human and rat gamma synuclein.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Human beta-endorphin is a 31 amino acid peptide cleaved from the precursor pro-opiomelanocortin (POMC). It is an endogenous opioid peptide neurotransmitter that interacts with opioid receptors.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized with BSA
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Synthetic human beta-endorphin peptide (1-31 aa) conjugated to BSA.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded
Antibody Isotype:
IgG
Application Details:
A dilution of 5-10 µg/mL is recommended for immunohistochemistry using formalin fixed and paraffin embedded tissues and for 4% paraformaldehyde fixed frozen tissues. A dilution of 5-15 µg/mL is recommended for immunofluorescence. For radioimmunoassay (RIA), a working dilution of 1:10,000 is recommended. Sensitivity - 120 pg/sample; IC50-1 ng/ sample. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
-endorphin; Pro-opiomelanocortin; POMC;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Confirmed by RIA against the synthetic peptide. Human; mouse; rat. Beta-endorphin is highly conserved so cross-reactivity with other species is expected. Cross-reactivity with other opioid peptides is as follows: with Met-enkephalin 0.03%; with Leu-enkephalin 0.02%; with beta-lipotropin 0.34%.
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 2-8°C with an appropriate antibacterial agent.Avoid freeze-thaw cycles
Fatty acid-binding protein, adipocyte (ALBP) is a lipid transport protein which binds long chain fatty acids and other hydrophobic ligands and delivers them to their receptors in the nucleus. ALBP is found in the cytoplasm and nucleus of adipocytes.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human
Immunogen:
Human Fatty acid-binding protein, adipocyte peptide (97-111 aa).
Applications:
WB
Antibody Isotype:
IgY
Application Details:
WB. Suggested dilution of 1:2,000-1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Chicken anti-Orexin A Polyclonal Antibody (Unconjugated), suitable for ELISA.
Background Info:
FUNCTION: Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity. SUBCELLULAR LOCATION: Endoplasmic reticulum; rough endoplasmic reticulum. Associated with perikaryal rough endoplasmic reticulum as well as cytoplasmic large granular vesicles at synapses. SIMILARITY: Belongs to the orexin family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A peptide from the N-terminus of human Orexin A (1-13 aa) .
Applications:
ELISA
Antibody Isotype:
IgY
Application Details:
ELISA . Suggested dilution at 1:2,000 to 1:5,000 as a detection antibody and 1:200 to 1:1,000 as a coating antibody in sandwich ELISA. Biosensis recommends that the optimal working dilution should be determined by the end user.
Goat anti-Mouse IgG (H&L), F(ab)'2 fragment, TRITC conjugated, min. cross-reactivity to bovine, goat, human, rabbit, or rat IgG is a secondary antibody conjugated to TRITC, which binds to mouse IgG (H&L), F(ab)'2 Fragment in immunological assays.
For reconstitution add 0,55 ml of sterile water,Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Antibody purity is > 90% based on SDS-PAGE Small amounts of intact IgG may be present.Affinity purified using solid phase Mouse IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free.Based on IEP, this antibody reacts with: • heavy (γ) chains on mouse IgG • light chains on all mouse immunoglobulins .Based on IEP, no reactivity is observed to: • non-immunoglobulin human serum immunoglobulins • bovine, goat, human, rabbit or rat IgG .
Chicken anti-Peptide YY (PYY) Polyclonal Antibody (Unconjugated), suitable for ELISA.
Background Info:
Peptide YY (PYY) is secreted from endocrine cells in the lower small intestine, colon and pancreas. PYY inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility (Ref: SWISS-Prot).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A peptide from the N-terminus of human mature Peptide YY (3-18 aa).
Applications:
ELISA
Antibody Isotype:
IgY
Application Details:
ELISA . Suggested dilution at 1:200 to 1:1,000 (coating antibody) and 1:2,000 to 1:5,000 (detection antibody) .
Rabbit anti-Beta-synuclein Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
Beta-synuclein is a non-amyloid component of senile plaques found in Alzheimer disease. It could act as a regulator of SNCA aggregation. It protects nerurons from staurosporine and 6 hydroxy dopamine -stimulated capspase activation in a p53-dependent manner. It localises to the cytoplasm and it is predominantly expressed in the brain where it is most concentrated in presynaptic nerve terminals. This protein is phosphorylated. This protein is also associated with the disease Brain iron accumulation type 1 (NBIA1).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (IEPLMEPEGSYEDPPQE) of human beta synuclein protein (aa: 108-125) conjugated to diptheria toxid has been used as the immunogen.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB , immunoblot. A dilution of 1:500 to 1:2000 is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
SNCB
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Israeli E. and Sharon R. (2009) Beta-synuclein occurs in vivo in lipid-associated oligomers and forms hetero-oligomers with alpha-synuclein J Neurochem. 2009 Jan;108(2):465-74
Specificity:
Less than 0.1% cross reactivity to human alpha synuclein This antiserum is known to react with human and rat beta synuclein.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Chicken anti-Peptide YY (PYY) Polyclonal Antibody (Unconjugated), suitable for ELISA.
Background Info:
Peptide YY (PYY) is secreted from endocrine cells in the lower small intestine, colon and pancreas. PYY inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility (Ref: SWISS-Prot).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A peptide from the C-terminus of human mature Peptide YY (24-36 aa) .
Applications:
ELISA
Antibody Isotype:
IgY
Application Details:
ELISA. Suggested dilution at 1:200 to 1:1,000 (coating antibody) and 1:2,000 to 1:5,000 (detection antibody). Biosensis recommends that the optimal working dilution should be determined by the end user.
Chicken anti-Presenilin-2 (PS-2) Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
Presenilin-2 (PSEN2) is a multi-pass membrane protein and component of the gamma-secretase complex. Defects in PSEN2 are a cause of Alzheimer disease type 4 (AD4), an autosomal dominant Alzheimer disease. (Ref:SWISS-Prot).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Mixture of two human Presenilin 2 peptides (319-330 and 349-360 aa). Both sequences are highly conserved in human, mouse and rat.
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
WB and ELISA. Suggested dilution of 1:1,000 to 1:4,000. To minimise background staining, a higher concentration of detergent (such as Tween-20) is suggested in the dilution and washing steps. Biosensis recommends that the optimal working dilution should be determined by the end user.
Rabbit anti-Clathrin Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Paraffin-embedded.
Background Info:
Clathrin is a major protein component of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice to either the plasma membrane or the trans-Golgi network. These specialised organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. Clathrin triskelions, composed of 3 heavy chains and 3 light chains, are the basic subunits of the clathrin coat. In the presence of light chains, hub assembly is influenced by both the pH and the concentration of calcium. Clathrin localises to the cytoplasmic face of coated pit and vesicle membranes.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Rat
Immunogen:
A synthetic peptide (KAGLLQRALEHFTDLYDIKR) corresponding to the amino acids 619-638 of rat clathrin conjugated to diphtheria toxin has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from clathrin protein in mouse, human and bovine.
Applications:
IHC-Paraffin-embedded,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB. This antibody works in immunohistochemistry on frozen or paraffin embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for routine immunohistochemistry are 1: 100 to 1: 4000 depending on tissue and detection method. It is usual to use approximately a 10-fold increase for confocal microscopy i.e. a dilution range of 1: 100 to 1: 400.For western blotting a dilution range of 1: 1000 to 1: 8000 is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Clathrin heavy chain; Clathrin heavy chain 1; CLH-17; CLTC
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antibody has been shown to be specific for clathrin. Rat
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent.
Chicken anti-Neurotrophin-4/5 (NT-4/5) Polyclonal Antibody (Unconjugated), suitable for ELISA.
Background Info:
FUNCTION: Target-derived survival factor for peripheral sensory sympathetic neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse
Immunogen:
Mixture of two human NT4/NT5 peptides (104-118 and 198-210 aa).
Applications:
ELISA
Antibody Isotype:
IgY
Application Details:
ELISA at suggested dilution of 1:500-1:2,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Chicken anti-Presenilin-1 (PS-1) Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
Presenilin-1 (PSEN1) is a multi-pass membrane protein and component of the gamma-secretase complex. PSEN1 is thought to play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. It may also play a role in hematopoiesis. Defects in PSEN1 are a cause of Alzheimer disease type 3 (AD3), a familial early-onset form of Alzheimer disease (Ref:SWISS-Prot).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Mixture of two human Presenilin 1 peptides (311-322 and 341-352 aa). Both sequences are highly conserved in human, mouse and rat.
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
WB and ELISA. Suggested dilution of 1:1,000 to 1:4,000. To minimise background staining, a higher concentration of detergent (such as Tween-20) is suggested in the dilution and washing steps. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
Presenilin 1; PS-1; Protein S182; PS1-CTF12; PSEN1; AD3; PS1; PSNL1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human Presenilin 1
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Fatty acid-binding protein, adipocyte (ALBP) is a lipid transport protein which binds long chain fatty acids and other hydrophobic ligands and delivers them to their receptors in the nucleus. ALBP is found in the cytoplasm and nucleus of adipocytes.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Mixture of two human Fatty acid-binding protein, adipocyte peptide peptides (71-82 aa, 97-111 aa).
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
WB and ELISA. Suggested dilution of 1:1,000-1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Goat anti-Mouse IgG (H&L), F(ab)'2 fragment, FITC conjugated, min. cross-reactivity to bovine, goat, human, rabbit, or rat IgG is a secondary antibody conjugated to FITC, which binds to mouse IgG (H&L), F(ab)'2 Fragment in immunological assays.
Antibody purity is > 90% based on SDS-PAGE Small amounts of intact IgG may be present.Affinity purified using solid phase Mouse IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free.Based on IEP, this antibody reacts with: • heavy (γ) chains on mouse IgG • light chains on all mouse immunoglobulins .Based on IEP, no reactivity is observed to: • non-immunoglobulin mouse serum immunoglobulins • bovine, goat, human, rabbit or rat IgG .
The amyloid beta peptide is derived from the cleavage of the Amyloid precursor protein (APP) and varies in length from 39 to 43 amino acids. However, the form(s) of amyloid-beta peptide (A? associated with the pathology characteristic of Alzheimer's disease (AD) remains unclear. In particular, the neurotoxicity of intraneuronal A? accumulation is an area of considerable research and controversy principally because antibodies thought to be specific for A? have been shown to actually detect intraneuronal APP and not A? exclusively.<br /><br />MOAB-2 (mouse IgG2b) is a pan-specific, high-titer antibody to A? residues 1-4 as demonstrated by biochemical and immunohistochemical analyses (IHC), and is highly specific just to amyloid beta peptide. <strong>MOAB-2 did not detect APP or APP-CTFs</strong> in cell culture media/lysates (HEK-APPSwe or HEK APPSwe/BACE1) or in brain homogenates from transgenic mice expressing 5 familial AD (FAD) mutation (5xFAD mice). <br /><br />Using IHC on 5xFAD brain tissue, MOAB-2 immunoreactivity co-localized with C-terminal antibodies specific for A?40 and A?42. MOAB-2 did not co-localize with either N- or C-terminal antibodies to APP. In addition, no MOAB-2-immunreactivity was observed in the brains of 5xFAD/BACE-/- mice, although significant amounts of APP were detected by N- and C-terminal antibodies to APP, as well as by 6E10. In both 5xFAD and 3xTg mouse brain tissue, MOAB-2 co-localized with cathepsin-D, a marker for acidic organelles, further evidence for intraneuronal A?, distinct from A? associated with the cell membrane. MOAB-2 demonstrated strong intraneuronal and extra-cellular immunoreactivity in 5xFAD and 3xTg mouse brain tissues.<br /><br />Biosensis now offers <strong>biotinylated MOAB-2</strong> <strong>antibody</strong> allowing more flexibility in experimental design by using the biotin-avidin/streptavidin detection method. Biotinylated MOAB-2 antibody may also help to reduce background staining in difficult-to-stain tissues and increase detection sensitivity. The ability of biotinylated MOAB-2 antibody to detect amyloid beta has been validated by IHC.<br /><br />Purified, non-biotinylated MOAB-2 antibody is available <a href="https://www.biosensis.com/moab-mouse-monoclonal-antibody-amyloid-beta-peptide-beta-4042-purified-p-1181.htmL">here</a>.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer, pH 7.4; contains no preservative.
Host Animal:
Mouse
Species Reactivity:
Human,Rat
Immunogen:
Recombinant human amyloid beta protein 42 (A?42): DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA
Applications:
ELISA,ICC,IHC-Frozen,IHC-Paraffin-embedded,IP,WB
Clone number:
MOAB-2
Antibody Isotype:
IgG2b, lambda
Application Details:
The biotinylated MOAB-2 antibody has been tested by IHC (1:500 - 1:2,000 dilution) and is also expected to work in applications validated for the unlabelled antibody (M-1586-100) at same or higher dilutions: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry/paraffin embedded IHC(P), Immunoprecipitation (IP), Immunofluorescence (IF), ELISA.<br><br><i>Western Blotting:</i><br><br>MOAB-2 has been tested in WB using purified synthetic beta-amyloid preparations and from transgenic mouse brain formic acid extracts (see Figure 1). Formic acid extraction/concentration is required for western blot detection from extracts. Suggested dilution of 1:2000-1:5,000 for WB, standard ECL detection systems. <br><br>Tissue samples for the detection of beta-amyloid should be prepared as detailed in Youmans KL et al., 2011 (Journal of Neuroscience Methods 196: 51-59) for best results. Detection of beta-amyloid 40/42 in direct westerns can be difficult; Dot-blots of prepared samples are recommended as detailed in Youmans KL et al., 2012. <br><br><i>Immunohistochemistry:</i><br><br>Suggested dilution for biotinylated MOAB-2 in IHC is 1:500-1:2,000. Fresh frozen, 4% paraformaldehyde fixed frozen, or formalin fixed paraffin embedded tissues are all suitable. Antigen retrieval is required in fixed tissues for optimal staining.<br><br>Antibody was tested on 4% paraformaldehyde/0.1% glutaraldehyde fixed frozen tissue from 3xTg and 5xFAD mice. MOAB-2 antibody detects intraneuronal and extracellular beta-amyloid in IHC and does not detect APP (Youmans KL et al., 2012).<br><br>The antibody also reacts with archival formalin-fixed, paraffin-embedded tissue samples with antigen Heat Induced Epitope Retrieval (HIER). Recommended buffer for HIER is citrate, pH 6.0. Signal was weak without antigen retrieval. Immunoreactivity was observed in intraneural-amyloid deposition (plaque) in Alzheimer's brain. MOAB-2 was found to be extremely clean and with an excellent signal to noise ratio with no neuro-cellular diffusive staining.<br><br>In addition, MOAB-2 demonstrated no significant differences in A-beta detection using paraffin fixed, free-floating sections (Youmans KL et al., 2012). Formic acid (FA) treatment resulted in optimal detection of both intraneuronal and extracellular A-beta compared to without FA (incubated in 88% FA 8 min, Youmans KL et al., 2012). Free floating tissue sections were permeabilized in TBS containing 0.25% Triton X-100 (TBSX; 3 x 10 min), blocked with 3% horse serum in TBSX (3 x 10 min) followed by 1% horse serum in TBSX (2 x10 min) and incubated with appropriate primary antibodies diluted in TBSX containing 1% horse serum overnight. See Youmans KL et al., 2012, for full IHC(P) protocol and method details.<br><br><i>Immunofluorescence:</i><br><br>For IF, suggested dilution is 1:100-1:500. The antibody was tested on 4% PFA fixed frozen tissue. Fixed tissues were washed in TBS (3 x 10 min), then incubated in 88% FA (8 min), and then permeabilized in TBSX (3 x 10 min), and blocked in TBSX containing 5% bovine serum albumin (BSA; 1 hr). Sections were subsequently incubated with appropriate primary antibodies diluted in TBSX containing 2% BSA overnight on an oscillatory rotator. Detection was via fluorescently labelled absorbed secondary antibodies (Youmans KL et al., 2012).<br><br><i>Immunoprecipitation:</i><br><br>For IP, the suggested dilution is 1:200 to 1:1,000 for labelled beta-amyloid using SA-coated beads as the capture vehicle, similar to the protocols employed by Youmans KL et al., 2012.<br><br><i>ELISA:</i><br><br>In an ELISA, a dilution of 1:50-1:1,000 is suggested. The antibody has been tested in ELISAs on synthetic beta-amyloid and tissue homogenates from beta-amyloid-Tg mice. <br><br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user for all applications. Dilutions provided are only meant to serve as a basic guide.
Kim, S. et al. (2020) Performance Validation of a Planar Hall Resistance Biosensor through Beta-Amyloid Biomarker. Sensors (Basel). 20(2) Application: In-vitro biosensor. Ruan, CS. et al. (2017) Sortilin inhibits amyloid pathology by regulating non-specific degradation of APP. Exp Neurol. [Epub ahead of print] Application: IHC References for non-biotinylated MOAB-2 antibody (M-1586-100): Zhu, B. et al. (2017) ER-associated degradation regulates Alzheimer's amyloid pathology and memory function by modulating _-secretase activity. Nat Commun. 8(1):1472. Application: IHC Huang, TY. et al. (2017) SORLA attenuates EphA4 signaling and amyloid _-induced neurodegeneration. J Exp Med. pii: jem.20171413. [Epub ahead of print]. Application: IHC Felecia, M. et al. (2017) Peripheral Inflammation, Apolipoprotein E4, and Amyloid-_ Interact to Induce Cognitive and Cerebrovascular Dysfunction. ASN Neuro. 9(4):1759091417719201. Application: IHC/IF Thomas, R. et al. (2016) Epidermal growth factor prevents APOE4 and amyloid-beta-induced cognitive and cerebrovascular deficits in female mice. Acta Neuropathol Commun. 4(1):111 Application: IHC Koster, KP. et al. (2016) Epidermal growth factor prevents oligomeric amyloid-_ induced angiogenesis deficits in vitro. J Cereb Blood Flow Metab. [Epub ahead of print] Application: IF Loffler, T. et al. (2016) Decreased Plasma A? in Hyperlipidemic APPSL Transgenic Mice Is Associated with BBB Dysfunction. Front. Neurosci. Application: IF Kobro-Flatmoen, A. et al. (2016) Reelin-immunoreactive neurons in entorhinal cortex layer II selectively express intracellular amyloid in early Alzheimer's disease. Neurobiology of Disease. 93:172-183. Application: IHC Tai, LM. et al. (2016) The role of APOE in cerebrovascular dysfunction. Acta Neuropathol. 131(5):709-23. Application: IF Kim, YH. et al. (2015) A 3D human neural cell culture system for modeling Alzheimer's disease. Nat Prot. 10(7):985-1006. Application: WB Condello, C. et al. (2015) Microglia constitute a barrier that prevents neurotoxic protofibrillar A?42 hotspots around plaques. Nat Commun. 6:6176. Application: IF Iulita MF et al (2014) Intracellular Abeta pathology and early cognitive impairments in a transgenic rat model overexpressing human amyloid precursor protein: a multidimensional study. Acta Neuropathol Commun. 6:61. Application: IF, IH Smith BR et al (2014) Neuronal inclusions of alpha-synuclein contribute to the pathogenesis of Krabbe disease. J Pathol. Apr;235(5):509-21. Application: IF
Specificity:
MOAB-2 detects preparations enriched in U-, O-, F-A?42, and U-A?40 by dot-blot, and is thus a pan-specific A? antibody. However, MOAB-2 is selective for the more neurotoxic A?42 compared to A?40. Indeed, MOAB-2 demonstrated a titration against antigen concentration, and detects A?40 at 2.5 pmol, but U-, O- and F-A?b42 at antigen concentrations as low as ~ 0.1 pmol (Youmans. KL et al., 2012; PMID: 22423893). MOAB-2 does not detect APP (Amyloid Precursor Protein). Human, rat, other species not yet tested. By Dot Blot, MOAB-2 detected rat A?40 and human A?40, albeit with less affinity than for A?42 (Youmans KL et al., 2012).
Storage:
After reconstitution keep aliquots at -20°C to -70°C for a higher stability. At 2-8°C keep up to one week; use sterile methods and pipettes. Highly purified glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles. Keep tightly closed when not in use and protected from light.
Purification:
Antibody was purified from cell culture supernatant by Protein G chromatography, biotinylated and buffer-exchanged into PBS, pH 7.4 buffer
Adaptins are important components of clathrin-coated vesicles transporting ligand-receptor complexes from the plasma membrane or from the trans Golgi network to lysosomes. Together with medium and small subunits, adaptins form a heterotetrameric complex called an adaptor whose role is to promote the formation of clathrin-coated pits and vesicles and to recognise sorting signals within the cytosolic tails of transmembrane cargo molecules. Gamma-adaptin protein belongs to the adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network and/or endosomes. SUBCELLULAR LOCATION: Golgi apparatus. Cytoplasmic vesicle, clathrin-coated vesicle membrane.
A synthetic peptide (IRLRELIRTIRTARTQAEER) corresponding to the amino acids 5-24 of human AP-1 complex subunit gamma-1 conjugated to diphtheria toxin has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from AP-1 complex subunit gamma-1 protein in mouse, rat, dog and zebra fish.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB. This antibody works in immunohistochemistry on frozen or wax embedded tissue. Antigen retrieval has been used in testing but may not be necessary. Typical working dilution: Light microscopy 1/500 to 1/2000 depending on tissue and detection method; Immunofluorescence 1/50 to 1/200; Western blotting 1/1000 to 1/4000 is recommended depending on detection method. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
AP-1 complex subunit gamma-1; Adapter-related protein complex 1 subunit gamma-1; Gamma1-adaptin; Adaptor protein complex AP-1 subunit gamma-1; Golgi adaptor HA1/AP1 adaptin subunit gamma-1; Clathrin assembly protein complex 1 gamma-1 large chain; AP1G1; ADTG; CLAPG1
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antibody has been shown to be specific for AP-1 Rat and human
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent.
Peroxisome proliferator-activated receptor gamma (PPARG) is a nuclear hormone receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the receptor binds to a promoter element in the gene for acyl-CoA oxidase and activates its transcription (Ref: SWISSPROT).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human
Immunogen:
Human Peroxisome proliferator-activated receptor gamma peptide (115-129 aa).
Applications:
WB
Antibody Isotype:
IgY
Application Details:
WB. Suggested dilution at 1:2,000 to 1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
PPAR gamma; PPAR-gamma; Nuclear receptor subfamily 1 group C member 3; PPARG; NR1C3;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human Peroxisome proliferator-activated receptor gamma
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Goat anti-Mouse IgG (H&L), F(ab)'2 Fragment, min. cross-reactivity to bovine, goat, human, rabbit, or rat IgG is a secondary antibody which binds to mouse IgG (H&L), F(ab)'2 Fragment in immunological assays.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Antibody purity is > 90% based on SDS-PAGE Small amounts of intact IgG may be present.Affinity purified using solid phase Mouse IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on mouse IgG • light chains on all mouse immunoglobulins .Based on IEP, no reactivity is observed to: • non-immunoglobulin mouse serum immunoglobulins • bovine, goat, human, rabbit or rat IgG .
Synthetic human Neurotensin peptide (151-163 aa) conjugated to BSA
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded
Antibody Isotype:
IgG
Application Details:
A dilution of 5-10 µg/mL is recommended for immunohistochemistry using formalin fixed and paraffin embedded tissues and for 4% paraformaldehyde fixed frozen tissues. A dilution of 5-15 µg/mL is recommended for immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Neuromedin N; NTS; NN; NmN; NT; NmN-125;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human; mouse; rat. Highly conserved and expected to interact with bovine, feline and monkey
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 2-8°C with an appropriate antibacterial agent. Avoid freeze-thaw cycles.
Chicken anti-Orexin B Polyclonal Antibody (Unconjugated), suitable for ELISA.
Background Info:
FUNCTION: Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity. SUBCELLULAR LOCATION: Endoplasmic reticulum; rough endoplasmic reticulum. Associated with perikaryal rough endoplasmic reticulum as well as cytoplasmic large granular vesicles at synapses. SIMILARITY: Belongs to the orexin family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A peptide from the N-terminus of human Orexin B (1-14 aa).
Applications:
ELISA
Antibody Isotype:
IgY
Application Details:
ELISA . Suggested dilution at 1:2,000 to 1:5,000 as a detection antibody and 1:200 to 1:1,000 as a coating antibody in sandwich ELISA. Biosensis recommends that the optimal working dilution should be determined by the end user.
Rabbit anti-Endothelin B receptor (ET-BR) Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
The Endothelin B receptor is a multi-pass membrane protein that belongs to the G-protein coupled receptor 1 family. It acts as a non-specific receptor for endothelin 1, 2, and 3.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A peptide from human Endothelin B receptor (299-313 aa).
Applications:
WB
Antibody Isotype:
IgG
Application Details:
WB. Suggested dilution of 1:1,000-1:5,000. Human Endothelin B receptor has a predicted length of 442 residues and MW of 50 kDa. Biosensis recommends that the optimal working dilution should be determined by the end user.
Chicken anti-Leptin Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
Leptin is secreted by white adipocytes and functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. Leptin also has several endocrine functions and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing (Ref: Entrez Gene) .
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Mouse,Rat
Immunogen:
Recombinant rat Leptin protein
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
ELISA and WB. Suggested dilution of 1:1,000-1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
Leptin; Obesity factor; Lep; Ob; Obese protein;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Rat Leptin
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse
Immunogen:
Mixture of two human BDNF peptides (73-87 and 194-208 aa). Both peptides are highly conserved in human and mouse.
Applications:
ELISA
Antibody Isotype:
IgY
Application Details:
ELISA. Suggested dilution at 1:500 to 1:2,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Rabbit anti-Leptin Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
Leptin is secreted by white adipocytes and functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. Leptin has a variety of other roles including endocrine functions, regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing (Ref Entrez Gene).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant human Leptin protein
Applications:
ELISA,WB
Antibody Isotype:
Mixed
Application Details:
WB and ELISA. Suggested dilution of 1:1,000-1:2,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
Leptin; Obesity factor; Lep; Ob; Obese protein;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human Leptin
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. FUNCTION: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. DISEASE: Defects in GDNF may be a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. DISEASE: Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Mixture of two human GDNF peptides (101-118 and 199-211 aa). Both peptides are highly conserved in human and mouse.
Applications:
ELISA,ICC,IHC-Frozen,WB
Antibody Isotype:
IgY
Application Details:
ELISA, WB and IHC. WB suggested dilution of 1:500-1:2,000. IHC suggested dilution of 1:50-1:500. Biosensis recommends that the optimal working dilution should be determined by the end user.
Rabbit anti-Somatostatin Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen, IHC-Paraffin-embedded, ICC.
Background Info:
Somatostatin is secreted by neuroendocrine neurons in hypothalamus and interacts with somatostatin receptors inhibiting secretion of somatotropin.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized with BSA
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Synthetic Somatostatin peptide (103-116) conjugated to BSA.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded
Antibody Isotype:
IgG
Application Details:
A dilution of 5-10 µg/mL is recommended for immunohistochemistry using formalin fixed and paraffin embedded tissues and for 4% paraformaldehyde fixed frozen tissues. A dilution of 5-15 µg/mL is recommended for immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Human Somatostatin. Cross reactivity is expected with mouse, rat, other species not yet tested.
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for up to six months for a higher stability and at 2-8°C for one week with an appropriate antibacterial agent. Avoid freeze-thaw cycles
Rabbit anti-Leptin Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
Leptin is secreted by white adipocytes and functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. Leptin has a variety of other roles including endocrine functions, regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing (Ref Entrez Gene).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Rabbit
Species Reactivity:
Mouse,Rat
Immunogen:
Recombinant rat Leptin protein
Applications:
ELISA,WB
Antibody Isotype:
Mixed
Application Details:
WB and ELISA. Suggested dilution of 1:1,000-1:4,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
Leptin; Obesity factor; Lep; Ob; Obese protein;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Mouse, Rat
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
The elF2c (Argonaute, Ago) protein is a core protein of the RNA-induced silencing complex (RISC).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence (845-860 aa) from the C-terminus of mouse elF2C conjugated to KLH.
Applications:
IHC-Frozen,IHC-Paraffin-embedded,IP,WB
Antibody Isotype:
IgG
Application Details:
Western Blotting (WB), Immunochemistry (IHC) and Immunoprecipitation (IP). The recommended concentration for IHC in formalin fixed and paraffin embedded tissues and formalin/acetone fixed tissues is 1:200-1:500. For WB, the recommended concentration is 1:1,000 to 1:2,000. The elFC2 protein has a predicted length of 860 residues and the MW of the monomer is 94 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Protein argonaute-1; Protein argonaute-2; Protein argonaute-3; Argonaute1; Argonaute2; Argonaute3; mAgo1; mAgo2; Eukaryotic translation initiation factor 2C; eIF-2C 2; eIF-2C 1; eIF2C 2; Piwi/argonaute family protein meIF2C2 Protein slicer; Eif2c2; Ago1; Ago2;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Huang KL, Chadee AB, Chen CY, Zhang Y, Shyu AB. (2013)"Phosphorylation at intrinsically disordered regions of PAM2 motif-containing proteins modulates their interactions with PABPC1 and influences mRNA fate." RNA. 2013 Jan 22. [Epub ahead of print]. Lugli G. et al (2008) Expression of microRNAs and their precursors in synaptic fractions of adult mouse forebrain. J Neurochem. Jul;106(2):650-61. Lugli G. et al (2005) Dicer and eIF2c are enriched at postsynaptic densities in adult mouse brain and are modified by neuronal activity in a calpain-dependent manner. J Neurochem. Aug;94(4):896-905.
Specificity:
Specificity has been confirmed by IHC, WB and IP in mouse brain. This antibody is expected to recognise at least elF2c-1, 2 and 3 since they have identical C-termini. Human; mouse; rat; The Ago proteins are highly conserved so cross-reactivity with other species is expected.
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 2-8°C with an appropriate antibacterial agent. Avoid freeze-thaw cycles.
Chicken anti-G-Protein B3 (Rho) Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
G-Protein b3 (GNB3) is a guanine nucleotide-binding protein (G protein). G-proteins are involved as a modulator or transducer in various transmembrane signaling systems (Ref: SWISSPROT).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Human GNB3 peptide (216-230 aa)
Applications:
WB
Antibody Isotype:
IgY
Application Details:
WB. Suggested dilution at 1:1,000 to 1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
The hormone Atrial natriuretic peptide (ANP) is produced by cardiomyocites and is secreted into the bloodstream. ANP acts on convoluted tubules in kidney reducing sodium absorption which results in reducing the blood pressure.
Synthetic rat Atrial natriuretic peptide (123-150 aa) conjugated to BSA.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded
Antibody Isotype:
IgG
Application Details:
A dilution of 5-10 µg/mL is recommended for immunohistochemistry using formalin fixed and paraffin embedded tissues and for 4% paraformaldehyde fixed frozen tissues. A dilution of 5-15 µg/mL is recommended for immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Human; mouse; rat. Highly conserved and expected to interact with feline, canine, porcine, bovine, and equine ANP.
Storage:
At least 12 months after purchase at 2 - 8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for up to 6 months for a higher stability and at 2-8°C with an appropriate antibacterial agent for up to 2 weeks.Avoid freeze-thaw cycles
FUNCTION: Ubiquitin-like protein which binds to a wide range of target proteins. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved in targeting RANGAP1 to the nuclear pore complex protein RANBP2. SUBUNIT: Covalently attached to a number of proteins such as PmL, RANGAP1, HIPK2, SP100, p53, p73alpha, MDM2, JUN and DNMT3B. Also interacts with HIF1A, HIPK2, HIPK3, CHD3, PIAS1, EXOSC9, TDG, RAD51 and RAD52. SUBCELLULAR LOCATION: Nucleus; nuclear membrane. Nucleus; nucleoplasm; nuclear speckle. Cytoplasm. SIMILARITY: Belongs to the ubiquitin family. SMT3 subfamily. SIMILARITY: Contains 1 ubiquitin-like domain.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (AKPSTEDLGDKKEGEY) as part of human SUMO-1 peptide (aa: 6-21) conjugated to diphtheria toxoid has been used as the immunogen. This antigen is homologous with SUMO-1 of rat.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB. A dilution of 1:1000 to 1:2000 is recommended for immunohistochemistry and 1:2000 to 1:4000 for western blot. Cell lysate from Hela and NIH-3T3 cell lysates may be used as a positive control, and for IHC, lung carcinoma may be used. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Small ubiquitin-related modifier 1; Ubiquitin-like protein SMT3C; SMT3 homolog 3; Ubiquitin-homology domain protein PIC1; Ubiquitin-like protein UBL1; GAP-modifying protein 1; GMP1; Sentrin; SUMO1; SMT3C; SMT3H3; UBL1
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antiserum recognises human SUMO-1 and not ubiquitin. This antiserum is known to cross react with rat and human SUMO-1.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Guinea Pig anti-Neuropeptide RFRP-3 Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
Neuropeptide VF is the precursor of neuropeptides NPSF (RFRP-1), RFRP-2 and RFRP-3 (NPVF). RFRP-3 is reported to inhibit forskolin-induced production of cAMP. RFRP-3 has also been shown to block morphine-induced analgesia.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Guinea Pig
Species Reactivity:
Human,Rat,Sheep
Immunogen:
A synthetic peptide (VPNLPQRF) corresponding to the amino acids 124-131 from human Neuropeptide VF. Neuropeptide VF is the precursor of the neuropeptides NPSF (RFRP-1), RFRP-2 and RFRP-3. The synthetic peptide was conjugated to a carrier protein KLH to enhance the immunological response.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
Immunohistochemistry (IHC). A concentration of 1 in 2000 is recommended. IHC performed in sheep brain (hypothalamus) demonstrates intense staining of cells and terminals. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity was demonstrated by immunohistochemistry. This antibody is known to react with rat and sheep.
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Guinea Pig anti-Oxytoxin Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
Oxytocin causes contraction of the smooth muscle of the uterus and of the mammary gland. It belongs to the vasopressin/oxytocin family. Oxytocin is secreted.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Guinea Pig
Species Reactivity:
Human,Sheep
Immunogen:
A synthetic peptide (CYIQNCPLG) corresponding to the amino acids 20-28 from human Oxytocin-neurophysin 1. This peptide was conjugated to carrier protein to enhance the immunological response.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A concentration of 1 in 2000 is recommended for IHC. IHC performed in sheep brain (hypothalamus) demonstrates intense staining of cells and terminals. No staining is evident when the primary antibody is pre-absorbed with 0.5 mg/mL of oxytocin. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
OT; OXT;OT-NPI; OT-NPI;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specificity was demonstrated by immunohistochemistry. This antibody is known to react with sheep. Other species have not yet been tested.
Storage:
It is recommended that a thawed sample is stored at 2-8°C for no longer than 2 weeks. Allocation of appropriate anti-bacterial agent can increase shelf life by several weeks. Diluted serum should be prepared as required. Long term stability requires storage, preferably in small aliquots at -20°C or lower. Glycerol (1:1) can be added to neat serum for additional stability if intended use does not prevent this.
Rabbit anti-Neurturin (NTN) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
Neurturin (NTN) is a member of the GDNF family of neurotrophic factors. This protein is a potent survival factor for several populations of central and peripheral neurons in mature and developing rodents. FUNCTION: Supports the survival of sympathetic neurons in culture. May regulate the development and maintenance of the CNS. Might control the size of non-neuronal cell population such as haemopoietic cells. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. DISEASE: Defects in NRTN are a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, and possibly with other loci, defects in NRTN are involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant human Neurturin (rh NTN)
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
Immunohistochemistry (IHC) at a suggested dilution of 1:2000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
NRTN; NTN;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specificity of this product was demonstrated by immunohistochemistry and dot blot showed no cross reactivity with GDNF. This antibody is known to react with human, mouse and rat.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Chicken anti-C-reactive protein (CRP) Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
C-reactive protein has several roles associated with host defence such as; promoting agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. It can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. COFACTOR: Binds 2 calcium ions per subunit. C-reactive protein exists as a homopentamer. There are 2 alternatively spliced isoforms. C-reactive protein is found in plasma and its concentration increases greatly during acute phase response to tissue injury, infection or other inflammatory stimuli. It is induced by IL-1 and IL-6.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human
Immunogen:
Purified human C-reactive protein
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
ELISA and WB. Suggested dilution of 1:2,000-1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
CRP; PTX1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human C-reactive protein Human
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Chicken anti-Leptin Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
Leptin is secreted by white adipocytes and functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. Leptin also has several endocrine functions and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing (Ref: Entrez Gene).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human
Immunogen:
Recombinant human Leptin protein
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
ELISA and WB. Suggested dilution of 1:1,000-1:2,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
Leptin; Obesity factor; Lep; Ob; Obese protein;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human Leptin
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Proopiomelanocortin (POMC) is the precursor of the melanocortin peptides alpha, beta and gamma. POMC neurons in the arcuate nucleus of the hypothalamus function as key central regulators of food intake and homeostatic control of energy balance in both rodents and non-human primates. MSH increases the pigmentation of skin by increasing the melanin production in melanocytes. MSH is produced in the pituitary gland and belongs to the POMC family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Guinea Pig
Species Reactivity:
Human,Rat,Sheep
Immunogen:
A synthetic peptide (Ac-SYSMEHFRWGKPV) corresponding to the amino acids 138-150 from human Proopiomelanocortin (POMC). POMC is the precursor of the melanocortin peptides alpha, beta and gamma. The synthetic peptide was conjugated to a carrier protein to enhance the immunological response.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A concentration of 1 in 2,000 is recommended for IHC. IHC performed in sheep brain (hypothalamus) demonstrates intense staining of cells. No staining is evident when the primary antibody is pre-absorbed with 0.5 mg/mL of alpha MSH. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity was demonstrated by immunohistochemistry. This antibody is known to react with human, rat and sheep. Other species have not yet been tested.
Storage:
It is recommended that a thawed sample is stored at 2-8°C for no longer than 2 weeks. Allocation of appropriate anti-bacterial agent can increase shelf life by several weeks. Diluted serum should be prepared as required. Long term stability requires storage, preferably in small aliquots at -20°C or lower. Glycerol (1:1) can be added to neat serum for additional stability if intended use does not prevent this.
Chicken anti-C-reactive protein (CRP) Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
C-reactive protein has several roles associated with host defence such as; promoting agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. It can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. COFACTOR: Binds 2 calcium ions per subunit. C-reactive protein exists as a homopentamer. There are 2 alternatively spliced isoforms. C-reactive protein is found in plasma and its concentration increases greatly during acute phase response to tissue injury, infection or other inflammatory stimuli. It is induced by IL-1 and IL-6.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Mouse
Immunogen:
A peptide from the C-terminus of mouse C-reactive protein (210-225 aa).
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
ELISA and WB. Suggested dilution of 1:2,000-1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
CRP; PTX1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
C-reactive protein Mouse
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Rabbit anti-llama IgG (H&L) - DyLight 550 Conjugate is a secondary antibody conjugated to DyLight 550, which binds to all llama IgG in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase llama IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on llama IgG light chains on all llama immunoglobulins.This antibody will react with VHH of llama IgG's.No reactivity is observed to: non-immunoglobulin llama serum proteins.
Application Details:
1 : 20-1 : 2000 for most applications (ELISA, Flow cytometry, Immunofluorescence, Immunolocalization)
Purity:
Immunogen affinity purified rabbit IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Rabbit anti-llama IgG (H&L) , DyLight 594 Conjugate is a secondary antibody conjugated to DyLight 594, which binds to all llama IgG (H&L) in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase llama IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on llama IgG light chains on all llama immunoglobulins.This antibody will react with VHH of llama IgG's.No reactivity is observed to: non-immunoglobulin llama serum proteins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified rabbit IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
IgA is a member of the antibody class of the immunoglobulin superfamily. There are several classes and subclasses (isotypes) of antibody, the antibody isotype being defined by the immunoglobulin heavy chain present in the molecule. The basic structure of an immunoglobulin molecule consists of two identical heavy chains (gamma , mu, alpha , delta , epsilon) and two identical light chains, either kappa or lambda. IgA contains the alpha -chain and may be present in a serum or secretory form. In serum, 90% of IgA is monomeric, while in its secretory form it is the main immunoglobulin found in secretions including tears, saliva, intestinal and bronchial mucous, sweat, colostrum, and secretions from the prostate and respiratory epithelia, where it has the job of defending exposed external surfaces of the body against attack from micro organisms. Secretory IgA is synthesized locally by plasma cells and dimerized intracellularly with a cysteine-rich J-chain. Clone N1CLA was developed to produce reduced background staining that is associated with polyclonal antibodies on paraffin sections.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
N1CLA
Concentration:
Greater than or equal to 46 mg/L
Storage buffer:
Tissue culture supernatant with Sodium azide
Storage:
2-8°C
References 1:
Merluzzi S et al. Blood Journal. 2010; 115(14):2810-2817
References 2:
Fagarasan S and Honjo T. Current opinion in Immunology. 2004; 16(3):277-283
References 3:
Pilette C et al. European Respiratory Journal. 2001; 18:571-588
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Mouse IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on mouse IgG • light chains on all mouse immunoglobulins .Based on IEP, no reactivity is observed to: • non-immunoglobulin mouse serum proteins • bovine, goat, human, rabbit or rat IgG Based on ELISA, this antibody has <1% reactivity to: • rat IgG .
Goat anti-Mouse IgG (H&L), HRP conjugated, min. cross-reactivity to bovine, goat, human, rabbit or rat IgG is a secondary antibody conjugated to HRP, which binds to mouse IgG (H&L) in immunological assays.
For reconstitution add 1,1 ml of sterile water,Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Mouse IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.1% (v/v) Kathon CG is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on mouse IgG • light chains on all mouse immunoglobulins .Based on IEP, no reactivity is observed to: • non-immunoglobulin mouse serum proteins • bovine, goat, human, rabbit or rat IgG Based on ELISA, this antibody has <1% reactivity to: • rat IgG .
Anti-IgM reacts with immunoglobulin mu (IgM) chains. IgM is one of the predominant surface immunoglobulins on B-lymphocytes. This antibody is useful when differentiating and sub-classifying hematolymphoid neoplasms.
Monosan Range:
MONOSAN Ready To Use
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Arnold A, et al. New Eng J Med. 1983; 309:1593-1599
References 2:
Leong AS, et al. Geenwich Medical Media Ltd. 1999; 217-219
References 3:
Taylor CR. Arch Path Lab Med. 1978; 102:113-121
References 4:
Kojima M, et al. APMIS. 2002; 110:875-80
References 5:
Pambuccian SE, et al. Am J Surg Pathol. 1997; 21:179-86
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Host:
Rabbit
Immunogen:
Purified goat IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.8, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on goat IgG · light chains on all goat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin goat serum immunoglobulins · IgG from human, mouse or rat
Country Of Origin:
Rabbit serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Desmin is a 53 kDa intermediate filament protein and exhibits a high degree of tissue specificity, its expression being predominantly confined to all types of muscle cells (cardiac, skeletal and smooth muscle). Regulation of desmin expression is stage and tissue-specific, since it is induced during terminal development and muscle cell differentiation. In skeletal en cardiac muscle cells desmin is localized in the Z-disk region and at the intercalated disk. The expression pattern of desmin in smooth muscle is much more heterogenous. Coexpression of desmin and vimentin has been observed in tumors derived from muscle tissue, i.e. rhabdomyosarcomas and leiomyosarcomas. Furthermore, during myocard dysfunction dramatic changes in the distribution of desmin have been observed. RCK106 reacts exclusively with Cytokeratin 18 in glandular epithelial cells of the digestive, respiRatory, and urogenital tracts, endocrine and exocrine cells and mesothelial cells, as well as adenocarcinomas originating from them.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Rabbit IgG, whole molecule
Buffer:
30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rabbit IgG · light chains on all rabbit immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rabbit serum proteins · serum proteins from bovine, goat, human, mouse, or rat · IgG from bovine, goat, human, mouse or rat
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Novel Juice is a non-mutagenic fluorescent reagent that produces instant visualization of DNA bands upon Blue Light or UV illumination of agarose gels. Supplied in GeneDireXs 6X DNA Loading Buffer, Novel Juice can be used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. We believe Novel Juice is the most sensitive stain available for detecting double-stranded DNA (dsDNA). It also contains three tracking dyes (Bromophenol Blue, Xylene Cyanol FF, and Orange G) for visually tracking the DNA migration on your gel without illumination. It is a safe , non-hazardous alternative to Ethidum Bromide, having been independently tested.
Background Info:
[APPLICATION] (1) Vortex Novel Juice for 10 seconds prior to use. (2) Dilute 1 part Novel Juice with 5 parts DNA sample or ladder and mix. (3) Load sample and run according to your standard lab procedures. (4) After electrophoresis, remove the gel and place it on UV or a visible-light transilluminator to visualize the bands. (5) Gels can also be post-stained with Ethidium Bromide if desired.
Gel Stain, Ethidium Bromide Alternative, DNA stain, nucleic acid dye, nucleic acid stain, DNA dye
Additional Info:
Contains Tracking Dyes: Bromophenol Blue, Xylene Cyanol FF, and Orange G. Store at room temperature or at 4°C up to 12 months. For longer periods, store at -20°C. Novel Juice Dye is light sensitive and should be stored protected from light.
Donkey anti-Mouse IgG (H&L), F(ab)'2 fragment, TRITC conjugate, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep IgG is a secondary antibody conjugated to TRITC, which binds to mouse IgG (H&L), F(ab)'2 Fragment in immunological assays.
For reconstitution add 0,55 ml of sterile water,Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Antibody purity is > 90% based on SDS-PAGE Small amounts of intact IgG may be present.Affinity purified using solid phase Mouse IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on mouse IgG • light chains on all mouse immunoglobulins .Based on IEP, no reactivity is observed to: • non-immunoglobulin mouse serum immunoglobulins • IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep .
Donkey anti-Mouse IgG (H&L), F(ab)'2 fragment, FITC conjugate, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep IgG is a secondary antibody conjugated to FITC, which binds to mouse IgG (H&L), F(ab)'2 Fragment in immunological assays.
Antibody purity is > 90% based on SDS-PAGE Small amounts of intact IgG may be present.Affinity purified using solid phase Mouse IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on mouse IgG • light chains on all mouse immunoglobulins .Based on IEP, no reactivity is observed to: • non-immunoglobulin mouse serum immunoglobulins • IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep .
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Storage:
2-8 °C
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and is highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery. Heat shock cognate protein 70 (HSC70), is a highly conserved protein and a member of the family of molecular chaperones.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid at 1 g/ l
Storage Temp:
Stable for at least one year at -20 °C. Avoid multiple freeze-thaw cycles, prepare aliquotes. Please, remember to spin a tube briefly prior opening them to avoid any loses that might occur from material adhering to the cap or sides of the tube.
Anti-CEA is employed as a tool to assist in the distinction between adenocarcinoma and mesotheliomas, along with other markers such as calretinin, CK 5/6, D2-40, HBME-1, Napsin A, MOC31, and Ber-EP4. Anti-CEA positivity is seen in adenocarcinomas from the lung and colon, as well.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN Ready To Use
Clone:
CEA31
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Go, VLW, et al., Cancer 1976;37:562-566
References 2:
Delellis, RA, et al., Am J Clin Pathol 1978;50:587-594
References 3:
Abutaily AS et al. J Clin Pathol. 2002 Sep;55(9):662-8
References 4:
Bhatnagar J et al. Anticancer Res. 2002;22(3):1849-57
References 5:
Carella R. et al. Am J Surg Pathol. 2001 Jan;25(1):43-50
The antibody marks cells of monocyte/macrophage lineage. This antibody is capable of staining monocytes, Kupffer cells, osteoclasts, granulocytes and their precursors; lymphomas are negative or show few granules. This antibody may be useful for the identification of myelomonocytic and histiocytic tumors. Since this detects a formalin-resistant epitope that may be associated with lysosomal granules, other lysosome-rich cells may also stain.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN Ready To Use
Clone:
Kp-1
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Anti-TTF-1 (Thyroid Transcription Factor 1) is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the organs rather than thyroid, germ cell tumors, and malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates. TTF-1 labeling is also seen in thyroid and thyroid-derived tumors. TTF-1 immunostaining is useful in the differentiation between pulmonary and nonpulmonary origin of adenocarcinomas in malignant effusions. TTF-1 staining is very reliable in discerning whether a brain metastasis has arisen from a pulmonary or nonpulmonary site, particularly when dealing with adenocarcinomas and largecell carcinomas.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN Ready To Use
Clone:
8G7G3/1
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Bejarano PA, et al. Mod Pathol. 1996; 9:445-52
References 2:
Di Loreto C, et al. Cancer Lett. 1998; 124:73-8
References 3:
Abutaily AS, et al. J Clin Pathol. 2002; 55:662-8
References 4:
Jang KY, et al. Anal Quant Cytol Histol. 2001; 23:400-4
Anti-CD45 (anti-leukocyte common antigen) is routinely used to aid the differential diagnosis of undifferentiated neoplasms, whenever malignant lymphoma is suspected by the morphological or clinical data. It is a highly specific antibody; therefore a positive result is highly indicative of hematolymphoid origin. Certain types of hematolymphoid neoplasms may lack CD45 (Hodgkin lymphoma, some T-cell lymphomas, and some leukemias) so its absence does not rule out a hematolymphoid tumor. This antibody is expressed almost exclusively by cells of hematopoietic lineage and is present in most benign and malignant lymphocytes as well as plasma cell precursors.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN Ready To Use
Clone:
2B11 & PD7/26
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Mason, DY, Am Pathol 1987;128:1-4
References 2:
Hall PA, Histopathology 1988;13:149-160
References 3:
Kurtin, PJ, Hum Path 1985;16:353-365
References 4:
Maluf HM et al. Mod Pathol. 1995 Feb; 8(2): 155-9
References 5:
Caballero T et al. J Clin Pathol. 1995 Aug;48(8): 743-8
Immunohistochemical methods have localized chromogranin in a wide variety of endocrine tissues including the pituitary, pancreas, thyroid, and parathyroid. Neuroendocrine cells exhibit a fine granular immunoreactivity to chromogranin. It is generally accepted that the co-expression of certain keratins and chromogranin mean neuroendocrine lineage. The presence of strong chromogranin staining and absence of keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN Ready To Use
Clone:
LK2H10
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Wilson, BS, et al., Am J Pathol; 115:458-468 (1984)
References 2:
Lyda MH, Weiss LM. Hum Pathol. 31(8):980-7 (2000)
References 3:
ontochristopoulous GJ et al. Dermatology.; 201(2):123-6 (2000)
References 4:
Qvigstad G et al. Histochem J.; 32(9):551-6 (2000)
Tumor associated glycoprotein (TAG)-72 is a high molecular weight glycoprotein that is present on the surface of many neoplastic cells, including adenocarcinomas of the breast, colon, and lung. TAG-72 is found in lung adenocarcinoma and is absent in mesothelioma, making the TAG-72 antibody useful in distinguishing adenocarcinoma from mesothelioma.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN Ready To Use
Clone:
B72.3
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Thor, A, et al. Cancer Res 1986;46:3118
References 2:
Schlom J, et al. Tumormarker Oncology;1987;2:3
References 3:
Osteen KG et al. In J Gynecol Pathol. 1992 Jul;11(3):216-20
References 4:
Ordonez NG. Am J Surg Pathol. 1998 Oct;22(10):1203-14
References 5:
Chhieng DC et al. Hum Pathol. 2003 Oct;34(10):1016-21
Smooth Muscle Actin is a part of the actin family of proteins which are highly conserved and form microfilaments. These filaments are one of the major components of the cytoskeleton. Anti-smooth muscle actin immunohistochemical reactivity is seen in smooth muscle cells, myofibroblasts and myoepithelial cells.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN Ready To Use
Clone:
1A4
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Cooke PH. A. J Cell Biol. 1976; 68:539-56
References 2:
Skalli O, et al. J Cell Biol. 1986; 103:2787-96
References 3:
Perez-Montiel MD, et al. Am J Dermatopathol. 2006; 28:105-11
Our Immunoplates are designed for the capture of exosomes from any biological sample. Our plates are a standard 96-well format allowing assays to be conducted as singles and/or multi-well. This enables easy optimisation of a wide range of sandwich ELISA assays or other downstream applications, such as RNA extraction and proteomic analyses. Transparent, white and black plates are available depending on the downstream detection approach (colorimetric, luminometric and fluorimetric). Uncoated and/or covalently-coated plates are also available if required.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Our Immunoplates are designed for the capture of exosomes from any biological sample. Our plates are a standard 96-well format allowing assays to be conducted as singles and/or multi-well. This enables easy optimisation of a wide range of sandwich ELISA assays or other downstream applications, such as RNA extraction and proteomic analyses. Transparent, white and black plates are available depending on the downstream detection approach (colorimetric, luminometric and fluorimetric). Uncoated and/or covalently-coated plates are also available if required.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Our Immunoplates are designed for the capture of exosomes from any biological sample. Our plates are a standard 96-well format allowing assays to be conducted as singles and/or multi-well. This enables easy optimisation of a wide range of sandwich ELISA assays or other downstream applications, such as RNA extraction and proteomic analyses. Transparent, white and black plates are available depending on the downstream detection approach (colorimetric, luminometric and fluorimetric). Uncoated and/or covalently-coated plates are also available if required.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Donkey
Immunogen:
Purified Sheep IgG, whole molecule
Buffer:
PBS, 1% BSA & 0.1% proclin 150
Preservative:
0.1% (v/v) Kathon CG
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on sheep IgG · light chains on all sheep immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin sheep serum proteins · IgG from human, mouse or rabbit
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Host:
Goat
Immunogen:
Purified Human IgA, (α chain)
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (α) chains on human IgA
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin human serum immunoglobulins · light chains on all human immunoglobulins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Rabbit
Immunogen:
Purified Goat IgG, Fc fragment
Buffer:
30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on goat IgG
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin goat serum immunoglobulins · light chains on all goat immunoglobulins · goat IgG, F(ab)2 fragment · human serum proteins
Country Of Origin:
Rabbit serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Rabbit
Immunogen:
Purified Hamster IgG, whole molecule
Buffer:
30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on hamster IgG · light chains on all hamster immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin hamster serum immunoglobulins
Country Of Origin:
Rabbit serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Donkey
Immunogen:
Purified Rat IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rat IgG · light chains on all rat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rat serum proteins · IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit or sheep
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Donkey
Immunogen:
Purified Rat IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20°C.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rat IgG · light chains on all rat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rat serum proteins · IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit or sheep
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Trademark:
DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Human IgA, (α chain)
Buffer:
30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (α) chains on human IgA
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin human serum immunoglobulins · light chains on all human immunoglobulins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Chicken
Immunogen:
Purified Goat IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on goat IgG · light chains on all goat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin goat serum immunoglobulins
Country Of Origin:
Chicken Ig fraction was prepared using serum from healthy hens of US origin.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Host:
Goat
Immunogen:
Purified Human IgM, µ chain
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 0.55 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (µ) chains on human IgM
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin human serum proteins · light chains on all human immunoglobulins · human IgA or IgG
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Host:
Goat
Immunogen:
Purified Human IgM, µ chain
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.8, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (µ) chains on human IgM
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin human serum proteins · light chains on all human immunoglobulins · human IgA or IgG
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified using solid phase Mouse IgM-kappa
Purity:
> 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Mouse IgM, µ chain
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Specificity:
Based on IEP, this antibody reacts with: · heavy (µ) chains on mouse IgM
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum proteins · light chains on all mouse immunoglobulins · human IgG or serum proteins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified using solid phase Human Plasminogen
Purity:
> 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Human Plasminogen
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Specificity:
Human Plasminogen
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Host:
Goat
Immunogen:
Purified Human IgM, µ chain
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (µ) chains on human IgM
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin human serum proteins · light chains on all human immunoglobulins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Chicken IgG/Y, whole molecule
Buffer:
30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity.
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on chicken IgG/Y · light chains on all chicken immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin chicken serum immunoglobulins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Donkey
Immunogen:
Purified Mouse IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20°C.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum immunoglobulins · IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Accession Number:
AAA51107
Trademark:
DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Rat IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.8, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rat IgG · light chains on all rat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rat serum proteins · IgG from human or mouse · highly cross absorbed against mouse IgG
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Rabbit
Immunogen:
Purified Mouse IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum proteins · human serum proteins
Country Of Origin:
Rabbit serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Donkey
Immunogen:
Purified Rabbit IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.8, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rabbit IgG · light chains on all rabbit immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rabbit serum proteins · mouse IgG or serum proteins
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Chicken
Immunogen:
Purified Mouse IgG, whole molecule
Buffer:
PBS, 1% BSA & 0.1% proclin 150
Preservative:
0.1% (v/v) Kathon CG
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum immunoglobulins
Country Of Origin:
Chicken Ig fraction was prepared using serum from healthy hens of US origin.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Donkey
Immunogen:
Purified Sheep IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20°C.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on sheep IgG · light chains on all sheep immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin sheep serum proteins · IgG from human, mouse or rabbit
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Trademark:
DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Donkey
Immunogen:
Purified Mouse IgG, whole molecule
Buffer:
PBS, 1% BSA & 0.1% proclin 150
Preservative:
0.1% (v/v) Kathon CG
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum immunoglobulins · IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Mouse IgG, whole molecule
Buffer:
30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum proteins · bovine, goat, human, rabbit or rat IgG
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Rabbit
Immunogen:
Purified Goat IgG, Fc fragment
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on goat IgG
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin goat serum immunoglobulins · light chains on all goat immunoglobulins · Goat IgG F(ab)'2 fragment
Country Of Origin:
Rabbit serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified using solid phase Guinea Pig IgG
Purity:
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Guinea Pig IgG, whole molecule
Buffer:
30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on guinea pig IgG · light chains on all guinea pig immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin guinea pig serum immunoglobulins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
This antibody reacts with Human IgE (ε chain). IgE evaluation of patients with suspected diseases associated with elevations in total immunoglobulin E (IgE), including allergic disease, primary immunodeficiencies, infections, malignancies, or other inflammatory diseases.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Human IgE, (ε) chain
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (ε) chains on human IgE
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin human serum immunoglobulins · light chains on all human immunoglobulins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Donkey
Immunogen:
Purified Rat IgG, whole molecule
Buffer:
PBS, 1% BSA & 0.1% proclin 150
Preservative:
0.1% (v/v) Kathon CG
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rat IgG · light chains on all rat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rat serum proteins · IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit or sheep
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
This antibody reacts with Human IgE (ε chain). IgE evaluation of patients with suspected diseases associated with elevations in total immunoglobulin E (IgE), including allergic disease, primary immunodeficiencies, infections, malignancies, or other inflammatory diseases.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Human IgE, (ε) chain
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.8, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (ε) chains on human IgE
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin human serum immunoglobulins · light chains on all human immunoglobulins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Rat IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rat IgG · light chains on all rat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rat serum proteins · IgG from human or mouse
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Human IgA, (α chain)
Buffer:
PBS, 1% BSA & 0.1% proclin 150
Preservative:
0.1% (v/v) Kathon CG
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.
Specificity:
Based on IEP, this antibody reacts with: · heavy (α) chains on human IgA
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin human serum immunoglobulins · light chains on all human immunoglobulins · serum proteins from bovine, mouse or rabbit
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Goat anti-Dog IgM Fc, Unconjugated is a secondary antibody which binds to dog IgM FC part in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Reconstituted material can be stoored in 4°Cup to 7 days.
Host Animal:
Goat
Species Reactivity:
Dog IgM, Fc part
Immunogen:
Purified normal IgM isolated from pooled dog serum
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail. No preservative added.
Application Details:
Immunoprecipitation, In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum, In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted in 2 mm diameter peripheral wells, Precipitin titre 1:64 when tested against pooled normal dog serum in agar-block immunodiffusion titration
Purity:
Protein G purified IgG.
Reconstitution:
For reconstitution add 1 ml of sterile water
Not reactive in:
The antibody does not cross-react with any other component of the dog immunoglobulin system
Goat anti-Dog IgG Fc, Unconjugated is a secondary antibody which binds to dog IgG FC part in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Reconstituted material can be stoored in 4°Cup to 7 days.
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of antibody has not been characterized in details. No preservative added, as it may interfere with the antibody activity.
Application Details:
Immunoprecipitation, In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum, In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted in 2 mm diameter peripheral wells, Precipitating titre 1:64 when tested against pooled normal dog serum in agar-block immunodiffusion titration
Purity:
Protein G purified goat IgG.
Reconstitution:
For reconstitution add 1 ml of sterile water
Not reactive in:
The antiserum does not cross-react with any other component of the dog immunoglobulin system
Nitrotyrosine can be detected in proteins from a variety of tissues, usually in association with pathological conditions. Reaction of nitric oxide with superoxide produces peroxynitrite, which can undergo heterolytic cleavage into nitronium and hydroxyl ions. Nitration of tyrosine residues by nitronium ion forms nitrotyrosine groups in the respective proteins. Nitrotyrosine is thus a marker for inflammation-associated tissue damage.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid at 1 mg/ml.
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Nitrotyrosine ,Nitrotyrosine
Immunogen:
NO2-Tyr-CH2-Thyroglobulin
Applications:
Immunohistochemistry (IHC) paraffin, Iimmunohistochemistry (IHC) frozen sections, Western blot (WB)
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Host:
Goat
Immunogen:
Purified Mouse IgG, whole molecule
Buffer:
30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum immunoglobulins · serum proteins from bovine, horse, human rabbit or swine
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified using solid phase Guinea Pig IgG
Purity:
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Guinea Pig IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20°C.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on guinea pig IgG · light chains on all guinea pig immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin guinea pig serum immunoglobulins · IgG from bovine, chicken, goat, hamster, horse, human, mouse, rabbit, rat or sheep
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Accession Number:
NP_001166520
Trademark:
DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Host:
Goat
Immunogen:
Purified Rat IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rat IgG · light chains on all rat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rat serum proteins · IgG from human or mouse · highly cross absorbed against mouse IgG
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Mouse IgG, whole molecule
Buffer:
30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum immunoglobulins · serum proteins from bovine, horse, human rabbit or swine
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Goat anti-mouse IgG (H&L) , DyLight 594 Conjugated, min. cross-reactivity to bovine, goat, human, rabbit, rat IgG (highly absorbed against rat IgG) is a secondary antibody conjugated to DyLight 594, which binds to all mouse IgG (H&L) in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase mouse IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG light chains on all mouse immunoglobulins.No reactivity is observed to: non-immunoglobulin mouse serum proteins IgG from bovine, goat, human, rabbit or rat.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in Human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 7.8. The individual Human Cytokeratins are numbered 1 to 20. The various epithelia in the Human body usually express Cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used in the distinction of different types of epithelial malignancies. The Cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
OVTL12/30
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Ramaekers, F. et al. 1990 Am J Pathol 136, 641-55
References 2:
van Niekerk, CC et al. 1991 J Pathol 165, 145-52
References 3:
van de Molengraft, F.J et al. 1993 Histopathology 22, 35-8
References 4:
van Niekerk, CC et al. 1997 Cancer Detect Prev 21, 247-57
Stem Cell Factor Receptor (SCFR) is a member of the receptor tyrosine kinase family. Binding of SCFR to its ligand Stem Cell Factor (PE-1242-5) induces receptor dimerization, autophosphorylation and signal transduction. SCFR is essential for the development of normal hematopoietic cells and plays an important role in the survival, proliferation and differentiation of mast cells, melanocytes and germ cells.
Product Type:
Protein
Format:
Purified SCFR consists of approximately 15-45% carbohydrate by weight.
Lyophilized products should be stored at 2 to 8°C. Following reconstitution, short-term storage at 2-8°C and longer-term storage of aliquots at -18 to -20°C is recommended. Repeated freeze thawing is not recommended.
Purification:
>95%, as determined by SDS-PAGE, visualised by Coomassie Brilliant Blue
Tyrosine Kinase Receptor B (TrkB)-Fc Chimera, Human, Purified Recombinant Protein, suitable for IP and to Block.
Background Info:
Tyrosine Kinase Receptor B (TrkB) is a high affinity receptor for brain-derived neurotrophic factor (BDNF) and neurotrophin-4/5. It also binds neurotrophin 3 (NT-3) with lower affinity. TrkB does not bind nerve growth factor (NGF). TrkB is involved in the development and/or maintenance of the nervous system and is widely expressed, predominantly in nervous tissue. TrkB is a heavily glycosylated type I transmembrane protein. The TrkB-Fc chimera contains 13 potential N-linked glycosylation sites.
Product Type:
Protein
Format:
The chimera consists of 20-40% carbohydrate by weight.
Applications:
Block,IP
Application Details:
The TrkB-Fc chimera bound to protein A sepharose beads is able to pull down its ligand BDNF. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Beros J et al. (2021) Age Related Response of Neonatal Rat Retinal Ganglion Cells to Reduced TrkB Signaling in vitro and in vivo. Front Cell Dev Biol. 9:671087. Application: Rat, Block. Beros J (2020) Pretreatment of ovaries with collagenase before vitrification keeps the ovarian reserve by maintaining cell-cell adhesion integrity in ovarian follicles. PhD Thesis. Application: Rat, Block.
Storage:
Lyophilized products should be stored at 2 to 8°C. Following reconstitution, short-term storage at 2-8°C is recommended and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Purification:
>95%, as determined by SDS-PAGE and visualized by silver stain.
Goat anti-Mouse IgG (H&L), DyLight 488 conjugated, min. cross-reactivity to bovine, goat, human, rabbit, rat IgG (higly absorbed against rat IgG) is a secondary antibody conjugated to DyLight 488, which binds to mouse IgG (H&L) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
For reconstitution add 1,1 ml of sterile water,Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Mouse IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on mouse IgG • light chains on all mouse immunoglobulins .Based on IEP, no reactivity is observed to: • non-immunoglobulin mouse serum proteins • bovine, goat, human, rabbit or rat IgG Based on ELISA, this antibody has <1% reactivity to: • rat IgG .
Chicken anti-Peptide YY (PYY) Polyclonal Antibody (Unconjugated), suitable for ELISA.
Background Info:
Peptide YY (PYY) is secreted from endocrine cells in the lower small intestine, colon and pancreas. PYY inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility (Ref: SWISS-Prot).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A peptide from the C-terminus of human mature Peptide YY (24-36 aa) .
Applications:
ELISA
Antibody Isotype:
IgY
Application Details:
ELISA . Suggested dilution at 1:2,000 to 1:10,000 (detection antibody). Biosensis recommends that the optimal working dilution should be determined by the end user.
EXO-Prep is a new and efficient method for exosome isolation from biofluids (i.e plasma serum and urine) and from cell culture supernatants. Exosomes are quicky isolated from your sample by an incubation step with EXO-Prep precipitation reagent. Exosome pellet is subsequently collected by one-step centrifugation and can be easily resuspended in PBS 1X. Solubilized exosomes are suitable for different downstream analyses such as proteomic or nucleic acids marker profiling, NTA, electron microscopy etc.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
EXO-Prep is a new and efficient method for exosome isolation from biofluids (i.e plasma serum and urine) and from cell culture supernatants. Exosomes are quicky isolated from your sample by an incubation step with EXO-Prep precipitation reagent. Exosome pellet is subsequently collected by one-step centrifugation and can be easily resuspended in PBS 1X. Solubilized exosomes are suitable for different downstream analyses such as proteomic or nucleic acids marker profiling, NTA, electron microscopy etc.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Chicken anti-Peptide YY (PYY) Polyclonal Antibody (Unconjugated), suitable for ELISA.
Background Info:
Peptide YY (PYY) is secreted from endocrine cells in the lower small intestine, colon and pancreas. PYY inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility (Ref: SWISS-Prot).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A peptide from the N-terminus of human mature Peptide YY (3-18 aa).
Applications:
ELISA
Antibody Isotype:
IgY
Application Details:
ELISA . Suggested dilution at 1:2,000 to 1:10,000 (detection antibody). Biosensis recommends that the optimal working dilution should be determined by the end user.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Dog IgG, whole molecule
Buffer:
PBS, 1% BSA & 0.1% proclin 150
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 0.55 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
2-8 °C
Shelf Life:
Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on dog IgG · light chains on all dog immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin dog serum immunoglobulins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Rat IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rat IgG · light chains on all rat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rat serum proteins · IgG from Goat, Hamster, Human, Mouse, or Rabbit · Serum from Bovine, Goat, Horse, Human, Mouse, or Rabbit
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Dog IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on dog IgG · light chains on all dog immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin dog serum immunoglobulins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
The human immunoglobulins consist of two identical heavy chains (~50 kD) and two identical light chains, which are linked together by disulphide bonds. The light chains can be either kappa or lambda. The five immunoglobulins IgA, IgD, IgE, IgG and IgM differ in their heavy chains, and IgA and IgM differ as they can occur in polymeric forms. The heavy chain of IgG is named the gamma-chain. In humans, IgG consists of four sub classes that differ only marginally in their amino acid composition. Antibodies to IgG have been reported to be useful in the identification of plasma cells, lymphoid cells containing IgG and classifying B cell derived neoplasms. The normal B cell population is polyclonal, expressing a range of different immunoglobulins. In contrast, the majority of B cell neoplasms are characterized by the proliferation of monoclonal cells expressing one type of light chain, whereas more than one type of heavy chain can be expressed by the same cell.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
RWP49
Concentration:
Greater than or equal to 59 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Karamchandani JR et al. American Journal of Clinical Pathology. 2012; 137: 699-711
References 2:
Janeway C et al.Chapter 3. Garland Science. New York and London. 2001
References 3:
Fridman W et al. FASEB Journal. 1991; 5: 2684-2690
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Dog IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.8, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on dog IgG · light chains on all dog immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin dog serum immunoglobulins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Cat IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.8, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on cat IgG · light chains on all cat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin cat serum immunoglobulins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Cat IgG, whole molecule
Buffer:
PBS, 1% BSA & 0.1% proclin 150
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 0.55 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
2-8 °C
Shelf Life:
Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on cat IgG · light chains on all cat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin cat serum immunoglobulins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Cat IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 0.55 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on cat IgG · light chains on all cat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin cat serum immunoglobulins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Creatine kinase is an enzyme consisting of three major isoenzymes, CK-BB (brain), CK-MB (heart), and CK-MM (skeletal muscle). CK-MM has been described as a marker for muscle damage in your heart, brain, or skeleton after a crush injury, seizures, muscular dystrophy, muscle inflammation, or another skeletal muscle disorder.
Concentration:
> 4.5 mg/ml (E 1% at 280 nm = 13.0)
Conjugate:
Unconjugated
Form:
Clear, colorless liquid, 0.2 µm filtered
Purification:
Affinity purified using solid phase Human CKMM
Purity:
> 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Human CKMM
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Specificity:
Based on ELISA: · Human CK-MM · Human CK-MB
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Olea europaea L.
Immunogen:
KLH-conjugated synthetic peptide chosen from available beta-conglutin sequences.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Zafra et al. (2018). Histological features of the olive seed and presence of 7S-type seed storage proteins as hallmarks of the olive fruit development. Front. Plant Sci., 12 October 2018 |
Dehydrin ERD10 belongs to dehydrin protein family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Lyophilized antibody can be stored at -20 °C for up to 3 years. Re-constituted antibody can be stored at 4°Cfor several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Brassica napus
Expected Species:
Brassica juncea, Brassica rapa, Capsella bursa-pastoris, Noccaea caerulescensSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from Brassica napus ERD10, UniProt: Q69BP4
CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where it serves as a multifunctional lipopolysaccharide receptor. It is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-18
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD20 is a cell surface 33-37 (depending on the degree of phosphorylation) kDa non-glycosylated surface phosphoprotein expressed on mature and most malignant B cells, but not stem cells or plasma cells (low number of the CD20 has been also detected on a subpopulation of T lymphocytes and it can be expressed on follicular dendritic cells). Its expression on B cells is synchronous with the expression of surface IgM. CD20 regulates transmembrane calcium conductance (probably functioning as a component of store-operated calcium channel), cell cycle progression and B-cell proliferation. It is associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following mAb treatment.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-97
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD45RA is a high molecular weight isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RA is expressed e.g. on naïve T cells and normal plasma cells.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
MEM-56
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD53 is a tetraspanin family transmembrane glycoprotein expressed in the lymphoid-myeloid lineage. This molecule has been reported to form complexes with other leukocyte surface proteins such as CD2, CD19, CD21, MHC II, VLA-4 or tetraspanins CD37, CD81 and CD82, thus probably modulating various signaling processes. CD53 is involved in radioresistancy of tumour cells and its triggering has anti-apoptotic effect. In thymus, CD53 is up-regulated in response to positive selection signals during T cell development, and is strongly expressed upon macrophage exposure to bacterial lipopolysaccharide, whereas stimulation of neutrophils results in down-regulation of CD53 expression.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-53
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
MEM-111
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-112
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD59 (protectin) is a small (18-20 kDa) GPI-anchored ubiquitously expressed inhibitor of the membrane attack complex (MAC). It is thus the key regulator that preserves the autologous cells from terminal effector mechanism of the complement cascade. CD59 associates with C5b-8 complex and thereby counteracts appropriate formation of cytolytic pore within the plasma membrane. CD59 is also an low-affinity ligand of human CD2 and causes T cell costimulation.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
MEM-43
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Rabbit IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20°C.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rabbit IgG · light chains on all rabbit immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rabbit serum proteins · serum proteins from bovine, goat, human, mouse, or rat · IgG from bovine, goat, human, mouse or rat
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Trademark:
DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Rabbit IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20°C.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rabbit IgG · light chains on all rabbit immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rabbit serum proteins · serum proteins from bovine, goat, human, mouse, or rat · IgG from bovine, goat, human, mouse or rat
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Trademark:
DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Rabbit IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20°C.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rabbit IgG · light chains on all rabbit immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rabbit serum proteins · serum proteins from bovine, goat, human, mouse, or rat · IgG from bovine, goat, human, mouse or rat
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Trademark:
DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Rabbit IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20°C.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rabbit IgG · light chains on all rabbit immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rabbit serum proteins · serum proteins from bovine, goat, human, mouse, or rat · IgG from bovine, goat, human, mouse or rat
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Trademark:
DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Host:
Rabbit
Immunogen:
Purified goat IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 0.55 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20°C.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on goat IgG · light chains on all goat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin goat serum immunoglobulins
Country Of Origin:
Rabbit serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Trademark:
DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Donkey
Immunogen:
Purified Goat IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20°C.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on goat IgG · light chains on all goat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin goat serum immunoglobulins · IgG from human, mouse, rabbit or rat
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Trademark:
DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Donkey
Immunogen:
Purified Goat IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20°C.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on goat IgG · light chains on all goat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin goat serum immunoglobulins · IgG from human, mouse, rabbit or rat
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Trademark:
DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Donkey
Immunogen:
Purified Goat IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20°C.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on goat IgG · light chains on all goat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin goat serum immunoglobulins · IgG from human, mouse, rabbit or rat
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Trademark:
DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Troponin I (Human Cardiac)-(Amino Acid Sequence 27-40)
Concentration:
4-6 mg/ml
Form:
Clear, colorless liquid, 0.2 µm filtered
Purification:
Affinity purified using solid-phase peptide 3 (-RAYATEPHAKKKS-)
Purity:
>95% based on HPLC
Host:
Goat
Immunogen:
Purified Human Cardiac Troponin, I subunit (Part# Hu-090-M)
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
36 Months
Specificity:
Human Cardiac Troponin I (cTNI) (Amino Acid Sequence 67-79)
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Country Of Origin:
US Origin
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkynje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Antibody Isotype:
IgG2a kappa
Monosan Range:
MONOSAN
Clone:
MEM-57
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD22, also known as Siglec-2 (sialic acid-binding immunoglobulin-like lectin-2) is a transmembrane glycoprotein binding alpha2,6-linked sialic acid-bearing ligands. Intracellular domain of CD22 recruits protein tyrosine phosphatase SHP-1 through the immunoreceptor tyrosine-based inhibitory motifs (ITIMs), thus setting a treshold for B cell receptor-mediated activation. CD22 also regulates B-cell response by involvement in controlling the CD19/CD21-Src-family protein tyrosine kinase amplification pathway and CD40 signaling. CD22 exhibits hallmarks of clathrin-mediated endocytic pathway.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-01
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD50 (intracellular adhesion molecule 3, ICAM-3) is a transmembrane glycoprotein expressed by leukocytes, that serves as a counter-receptor for the lymphocyte function-associated antigen (LFA)-1 integrin. Besides functioning as an adhesive molecule that mediates e.g. the contact between T cells and antigen presenting cells, ICAM-3 regulates affinity of LFA-1 for ICAM-1 and induces T cell activation and proliferation. ICAM-3 plays an essential role in the initiation of the immune response both on T cells and antigen presenting cells and interacts also with CD209 (dendritic cell-specific ICAM-3-grabbing nonintegrin, DC-SIGN), a C-type lectin of dendritic cells and macrophages; this process is involved in dialogue between dendritic cells and granulocytes.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-171
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where it serves as a multifunctional lipopolysaccharide receptor. It is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-15
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD29 (beta1 integrin subunit, GPIIa) forms non-covalently linked heterodimers with at least 6 different alpha chains (alpha1-alpha6, CD49a-f) determining the binding properties of beta1 (VLA) integrins. These integrins mediate cell adhesion to collagen, fibronectin, laminin and other extracellular matrix (ECM) components. This interaction hinders cell death, whereas disruption of anchorage to ECM leads to apoptosis. Decreased expression of most beta1 integrins correlates with acquiring multidrug resistance of tumour cells during selection in presence of antitumour drug. In platelets, translocation of intracellular pool of beta1 integrins to the plasma membrane following thrombin stimulation. These integrins are also up-regulated in leukocytes during emigration and extravascular migration and appear to be critically involved in regulating the immune cell trafficking from blood to tissue, as well as in regulating tissue damage and disease symptoms related to inflammatory bowel disease. Through a beta1 integrin-dependent mechanism, fibronectin and type I collagen enhance cytokine secretion of human airway smooth muscle in response to IL-1beta.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-101A
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
The antibody HL-37 reacts with polymorphic determinant on human HLA-DQ1 and HLA-DQ3 molecules (recognized epitope was found on isolated beta chain of DQ1), but does not react with HLA-DQ2. DQ is the isotype of human MHC Class II molecules expressed on antigen-presenting cells (APC; dendritic cells, B lymphocytes, monocytes, macrophages).
Antibody Isotype:
IgG3
Monosan Range:
MONOSAN
Clone:
HL-37
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD48 (Blast-1) belongs to the CD2 subset of the Ig superfamily, which includes CD2, CD2F-10, CD58, CD84, CD150, CD229, CD244 and others. These molecules bind to the same or another members of their family, thus mediate homotypic or heterotypic adhesion. CD48 is a GPI-anchored protein broadly expressed on hematopoietic cells and serves as a high affinity ligand for 2B4 and low affinity ligand for CD2. 2B4-CD48 interaction among NK cells and NK-T cells regulates cell proliferation. Signaling through CD48 results in eosinophil activation and CD48 expression is increased in several infectious diseases.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-102
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
MEM-115
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
The CD8 T cell coreceptor (monomer approx. 32-34 kDa) is expressed as alpha/beta heterodimer on majority of MHC I-restricted conventional T cells and thymocytes and as alpha/alpha homodimer on subsets of memory T cells, intraepithelial lymphocytes, NK cells and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha-beta but not alpha-alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
MEM-31
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD10 (neutral endopeptidase – NEP, common acute lymphocytic leukemia antigen – CALLA, membrane metallo-endopeptidase – MME, enkefalinase) is a 100-kDa cell surface zinc metalloprotease, cleaving peptide bonds on the N-terminus of hydrophobic amino acids and inactivating multiple physiologically active peptids. CD10 is expressed on various normal cell types, including lymphoid precursor cells, germinal center B lymhocytes, and some epithelial cells, and its expression level serves as a marker for diagnostics of many carcinomas. CD10 is also a differentiation antigen for early B-lymphoid progenitors in the B-cell differentiation pathway and has a key role in regulation of growth, differentiation and signal transduction of many cellular systems.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-78
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation; binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-25
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD17, lactosylceramide, is an ubiquitous glycosphingolipid with uncharged disaccharide headgroup, highly enriched in lipid raft-derived structures. Besides playing a pivotal role in the biosynthesis of complex glycosphingilipids, lactosylceramide is involved in cell-cell and cell-matrix interactions and in signaling events linked to cell differentiation, development, apoptosis and oncogenesis. Lactosylceramide regulates integrin functions and production of nitric oxide. Its expression defines successive stages in the maturation of myeloid cells.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
MEM-68
Concentration:
1 mg/ml
Storage buffer:
Tris buffered saline (TBS) solution with 15 mM sodium azide
ACTH or Adrenocorticotropic hormone is synthesized from pre-pro-opiomelanocortin (pre-POMC). ACTH is produced and secreted from corticotrophs in the anterior lobe (or adenohypophysis) of the pituitary gland. The anti-ACTH immunohistochemical reagent could be useful in the study of neoplastic and non-neoplastic pituitary diseases
Monosan Range:
MONOSAN Ready To Use
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Pizarro CB, et al. Braz J Med Biol Res. 2004; 37:235-43
References 2:
Kageyama K, et al. Am J Med Sci. 2002; 324:326-30
References 3:
Fan X, et al. J Histochem Cytochem. 2002; 50:1509- 16
References 4:
Japon MA, et al. J Clin Endocrinol Metab. 2002; 87:1879-84
CD206 (macrophage mannose receptor, MMR), also known as mannose receptor C1 (MRC1), is a type I transmembrane glycoprotein serving as pattern recognition receptor for carbogydrate groups on the surface of bacteria, fungi and other pathogens. Expressed mainly on tissue macrophages and dendritic cells, CD206 mediates endocytosis of these pathogens and presentation of their antigens to the adaptive immune system. CD206 can also be detected in a soluble form in human plasma and is elevated in patients with acute sepsis.
Transferrin is a monomeric glycoprotein of approximately 77 kDa, which serves as an iron-transporter. In normal plasma, transferrin has a concentration of 25-50 µmol / liter, and is usually about one-third saturated with iron, thus providing a large buffering capacity in case of an acute increase in plasma iron levels. Cells take up transferrin-iron complexes (holotransferrin) using transferrin receptor dimers. Upon binding of holotransferrin, the receptor is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with its receptor (CD71) and recycles to the cell surface, where apotransferrin is released upon exposure to normal pH. Internalization of labeled transferrin thus represents an usefull approach to study endocytosis. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. Iron/transferrin complex is essential in haemoglobin synthesis and for certain types of cell division.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
HTF-14
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage:
2-8°C
References 1:
Bartek J., et al., Immunol. Lett. 1982; 7: 231.
References 2:
Bartek, J., et al., Br. J. Haematol 1985; 59: 435-441.
References 3:
Nováková, M., et al., Cell Motil Cytoskeleton 1996;33(1):38-51
From every molecule of proinsulin, one molecule of insulin plus one molecule of C-peptide are produced. C-peptide is released into the blood stream in equal amounts to insulin.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
C-PEP-01
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
MON5023 reacts only with mature insulin and has no reactivity with the C-Peptide and insulin chains. Insulin and glucagon are pancreatic endocrine hormones secreted by islet cells within the pancreas. The stimulus for insulin secretion is a HIGH blood glucose. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
IN-05
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Human serum albumin (65-67 kDa) is the most abundant protein in human blood plasma (produced in the liver). It has a serum half-life of approximately 20 days.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
AL-01
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms – FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
CD146, also known as MCAM (melanoma cell adhesion molecule) or MUC18, is a heavily glycosylated transmembrane glycoprotein with more than 50% of the mass from carbohydrates. It is expressed on epithelial and endothelial cells, fibroblasts, multipotent mesenchymal stromal cells, activated T cells and activated keratinocytes, and on some cancer cells, especially melanoma. The presence of CD146 on circulating blood cells has been confined to the activated T cells rather than circulating endothelial cells. CD146 mediates heterophilic cell adhesion and regulates monocyte transendothelial migration.SpecificityThe antibody MEM-M6/2 recognizes extracellular Ig domain D1 of CD147 (Neurothelin), a 50-60 kDa type I transmembrane glycoprotein primarily expressed on all leukocytes, red blood cells, platelets and endothelial cells; it is not expressed by resting lymphocytes.
The antibody MEM-M6/1 is a high-affinity antibody capable of binding to unstimulated peripheral blood T cells.Application detailsWestern blotting: Nonreducing conditions. <br>Flow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
P1H12
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Human CD6 antigen purified by immunoaffinity chromatography from HBP-ALL cells followed by preparative SDS-PAGE of non-boiled non-reduced sample (excised piece of gel corresponding to the 100 kDa zone).
CD6, also known as T12, is a member of the scavenger receptor superfamily found on T and B cell subsets, thymocytes, and acute lymphocytic leukemia cells (ALL). CD6 interacts with its ligand CD166/ALCAM (activated leukocyte cell adhesion molecule) and serves as a coreceptor for T cell activation and stabilizer of the immunological synapse. CD6-ALCAM mediated cell adhesion is also important for T cell proliferation. CD6 may exert some its functions via association with CD5, probably by fine-tuning CD5 signaling. Ligation of CD6 has antiapoptotic role in chronic lymphocytic leukemia B cells.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-98
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
TNF-alpha is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells. It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha easily forms stable trimers, but also other multimeric complexes. In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.SpecificityThe mouse monoclonal antibody TPX2-01 recognizes the epitope EPFVPKKEKKS (aa 636-646 in human) of TPX2, a microtubule-associated intracellular critical regulator of mitosis, which is overexpressed in many types of tumors and is a marker of worse prognosis in various cancers.Application detailsELISA: Biotinylated MAb11 can be used as a detection antibody in combination with capture antibody MAb1. <br>Immunohistochemistry (frozen sections): paraformaldehyde-fixed, saponin-treated frozen tissue sections. <br>Flow cytometry: Intracellular staining.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
MAb11
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD117 / c-Kit (stem cell factor receptor) is a 145 kDa receptor tyrosine kinase that regulates cell proliferation, adhesion, chemotaxis, apoptosis and other cell processes. Mutations of CD117 / c-Kit can lead to growth and progression of tumours. After binding of its ligand, SCF (stem cell factor), CD117 / c-Kit is autophosphorylated on its intracellular domains and activated. CD117 is expressed on pluripotent hematopoietic progenitor cells, mast cells and various cancer cells, e.g. acute myeloid leukemia cells.SpecificityThe mouse monoclonal antibody 12D3 recognizes an extracellular epitope of CD118, a transmembrane glycoprotein, which associates with CD130 to form the functional high affinity LIF receptor.Application detailsFlow cytometry: Recommended dilution: 1 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
104D2
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD116 (GM-CSF R alpha) is the low affinity receptor for granulocyte-macrophage colony-stimulating factor (GM-CSF). CD116 heterodimerizes with CD131, the common beta chain subunit shared with IL-3 and IL5- receptors, to form the high affinity GM-CSF receptor. CD116 is expressed by myeloid cells including macrophages, neutrophils, eosinophils, dendritic cells, and their precursors, as well as on endothelial cells. It is being used as a specific marker of myeloid leukemias.SpecificityThe mouse monoclonal antibody 104D2 detects extracellular part of CD117 / c-Kit protooncogen.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
4H1
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
CD114 / G-CSFR (granulocyte colony-stimulating factor receptor, also known as CSF3R) is a type I transmembrane glycoprotein which upon binding of its ligand (G-CSF, granulocyte colony-stimulating factor) homodimerizes and activates signaling transduction to mediate cell proliferation, survival, and differentiation. It is expressed by granulocytes at all stages of their differentiation, as well as by monocytes, dendritic cells, and mature platelets. Among non-hematopoietic cells, it is expressed e.g. by endothelial cells, placenta, trophoblasts, and many tumor cell lines. This antigen is a target for stem cell mobilization for blood stem cell transplantation, for enhancing recovery of myelopoiesis following chemotherapy and in the treatment of patients with severe chronic neutropenia.SpecificityThe mouse monoclonal antibody 4H1 recognizes an extracellular epitope of human CD116, the GM-CSF receptor alpha subunit (approx. 80 kDa) expressed e.g. by neutrophils, eosinophils, monocytes and macrophages.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
LMM741
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD112, also known as nectin-2, is a transmembrane glycoprotein involved in organization of adherens junctions. It also serves as a target molecule for entry of certain strains of herpes simplex virus (HSV) and pseudorabies virus (PRV). It is homologous to CD155, which serves as a target molecule for polio virus. CD112 seems to play a role in neural tube formation, with N-cadherin. Inside the cell, CD112 is connected with actin cytoskeleton through afadin. Variations in the CD112 gene have been associated with differences in the severity of multiple sclerosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.SpecificityThe mouse monoclonal antibody LMM741 recognizes an extracellular epitope of CD114 (colony stimulating factor 3 receptor), a 130 kDa transmembrane glycoprotein expressed on granulocytes and their differentiation stages, on monocytes, platelets, endothelial cells and placenta. It is absent from lymphocytes and erythrocytes.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
R2.525
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD111, also known as nectin-1, is a calcium-independent cell-cell adhesion transmembrane glycoprotein involved in organization of adherens junctions and tight junctions in epithelial and endothelial cells. It also serves as a target molecule for entry of herpes simplex virus (HSV-1, HSV-2) and pseudorabies virus (PRV) into epithelial and neuronal cells. CD111 is connected with actin cytoskeleton through afadin. Mutations in the gene for CD111 cause cleft lip and palate/ectodermal dysplasia 1 syndrome (CLPED1) as well as non-syndromic cleft lip with or without cleft palate (CL/P). Alternative splicing results in multiple transcript variants encoding proteins with distinct C-termini.SpecificityThe mouse monoclonal antibody R2.525 recognizes an extracellular epitope on CD112, a type I transmembrane glycoprotein expressed by myelomonocytic and megakaryocytic cells, and by CD34+ hematopoietic progenitors.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
R1.302
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD106 / VCAM-1 (vascular cell adhesion molecule-1) is an Ig-like cell surface adhesion molecule binding VLA-4 integrin. VCAM-1 is a potent T cell costimulatory molecule taking part in their positive selection and survival, as well as in adhesion, transendothelial migration and activation of peripheral T cells. VCAM-1 is also involved in endothelial cell-cell contacts. Whereas VCAM-1 normally mediates leukocyte extravasion to sites of tissue inflammation, tumour cells can use overexpressed VCAM-1 to escape T cell immunity. Soluble form of VCAM-1 (sVCAM-1) is an inflammatory marker and can be used also in prognosis of subsequent cariovascular events following acute coronary syndromes.SpecificityThe mouse monoclonal antibody W7C5 recognizes CD109, an approximately 165 kDa GPI-anchored extracellular protein expressed mainly on various hematopoietic cells, activated T lymphoblasts, activated platelets, and endothelial cells.Application detailsFlow cytometry: Recommended dilution: 4-6 ?g/ml; positive control: TNF-alpha activated HUVEC cells. <br>Immunohistochemistry (frozen sections): Acetone fixation. <br>ELISA: Capture mAb for soluble CD106.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
STA
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD103 / integrin alphaE is an integrin subunit that is expressed on intraepithelial lymphocytes, epithelial dendritic cells, lamina propria-derived dendritic cells, a subpopulation of lamina propria T cells, a small subset of peripheral lymphocytes, namely T reg cells, and on activated and TGF-beta stimulated lymphocytes. CD103 is in mature form cleaved into two disulfide-linked chains (C-terminal 150 kDa chain and N-terminal 25 kDa chain). It heterodimerizes with integrin beta7 subunit to form alphaE/beta7 integrin (mucosal lymphocyte 1 antigen), which through binding E-cadherin mediates homing of lymphocytes to the intestinal epithelium, and, in addition to the role in adhesion, may serve as an accessory molecule for intraepithelial lymphocyte activation.SpecificityThe antibody MEM-229 recognizes an extracellular epitope of CD105 (Endoglin), a 90 kDa type I integral membrane homodimer glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow; it is also present on syncytiotrophoblast on placenta throughout pregnancy.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
Ber-ACT8
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD102 / ICAM-2 (intracellular cell adhesion molecule-2), a counter receptor of LFA-1 (CD11a/CD18), is a transmembrane glycoprotein with two extracellular IgC-like domains and intracellular C-terminal tail. It is involved in lymphocyte recirculation and homing to the sites of inflammation. Through interaction with integrins it provides to the immune cells costimulatory signals. Expression of CD102 on blood cells (lymphocytes, monocytes, thrombocytes) is lower than on endothelium and follicular dendritic cells. CD102 levels are upregulated in lymph nodes with malignant infiltration.SpecificityThe mouse monoclonal antibody Ber-ACT8 recognizes an extracellular epitope of CD103 (alpha E integrin), a type I transmembrane glycoprotein primarily found on intestinal intraepithelial lymphocytes.Application detailsFlow cytometry: Recommended dilution: 2-4 ?g/ml.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
CBR-IC2/2
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD101 is a type I transmembrane glycoprotein, which forms disulfide-linked homodimers. It is expressed on activated T cells, as well as on granulocytes, monocytes, dendritic cells or mucosal T cells. It plays a major role in the activation of T cells by skin dendritic cells. Function of CD101 has not been fully elucidated, but in mice its knock-out results in liver autoimmune disease induced by Novosphingobium aromaticivorans.SpecificityThe mouse monoclonal antibody CBR-IC2/2 recognizes an extracellular epitope of CD102 (ICAM-2), an approximately 55 kDa type I transmembrane glycoprotein expressed mainly on vascular endothelial cells and folicular dendritic cells, in lower amount on lymphocytes, monocytes and platelets.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
BB27
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD100, also known as semaphorin 4D, is a homodimerizing type I transmembrane glycoprotein containing an extracellular semaphorin domain. It is expressed on most hematopoietic cells with the exception of immature bone marrow cells, erythrocytes and platelets. A 120 kDa soluble form is generated from the transmembrane form by proteolytic cascade following primary T and B cell activation. It seems CD100 acts through dampening CD72-mediated negative signaling. CD100 promotes angiogenesis, invasive growth, proliferation and anti-apoptosis of cancer cells in vitro. Higher expression levels of CD100 correlate with poor survival in soft tissue sarcoma patients.SpecificityThe mouse monoclonal antibody BB27 recognizes an extracellular epitope of CD101, a 140 kDa disulfide-bonded homodimeric protein expressed on activated T cells, and some other cell types, such as granulocytes and cells of the monocyte/macropgage lineage.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
133-1C6
Concentration:
1 mg/ml
Format:
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD10 (neutral endopeptidase – NEP, common acute lymphocytic leukemia antigen – CALLA, membrane metallo-endopeptidase – MME, enkefalinase) is a 100-kDa cell surface zinc metalloprotease, cleaving peptide bonds on the N-terminus of hydrophobic amino acids and inactivating multiple physiologically active peptids. CD10 is expressed on various normal cell types, including lymphoid precursor cells, germinal center B lymhocytes, and some epithelial cells, and its expression level serves as a marker for diagnostics of many carcinomas. CD10 is also a differentiation antigen for early B-lymphoid progenitors in the B-cell differentiation pathway and has a key role in regulation of growth, differentiation and signal transduction of many cellular systems.SpecificityThe mouse monoclonal antibody 133-1C6 recognizes an extracellular epitope of CD100, an approximately 150 kDa (when reduced) semaphorin family member expressed mainly on lymphocytes, NK cells, monocytes/macrophages and granulocytes, but also on some non-hematopoietic cells.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
LT10
Concentration:
1 mg/ml
Format:
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Beta2-microglobulin non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.SpecificityThe mouse monoclonal antibody HC3aRZ8 recognizes an extracellular epitope of C3aR, a transmembrane chemotactic receptor for C3a anaphylatoxin.Application detailsImmunohistochemistry (paraffin sections): Recommended dilution: 5 ?g/ml; positive tissue: liver. <br>Western blotting: Recommended dilution: 2 ?g/ml; non-reducing conditions preferred. <br>Flow cytometry: Recommended dilution: 1 ?g/ml; positive control: PBL cell line, negative control: DAUDI human lymphoma cell line, erythrocytes. <br>ELISA: Working dilution should be determinated by investigator.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
B2M-02
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
C-11
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratin 18 belongs to type I family (acidic cytokeratins).
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
DA-7
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
C-43
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
C-51
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
C-50
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratin 18 belongs to type I family (acidic cytokeratins).
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
DC-10
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
C-46
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
SpecificityThe antibody MEM-E/07 recognizes an extracellular epitope on native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells.
The published results revealed that the antibody cross-reacts with some classical MHC Class I molecules (MHC Class Ia): HLA-B7 (strongly), HLA-B8 (moderately), HLA-B27, -B44 (weakly).Application detailsWestern blotting: Non-reducing conditions. <br>Flow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
HL-38
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.SpecificityThe mouse monoclonal antibody BB7.1 recognizes an extracellular antigen of HLA-B7 antigen. Although highly specific, it can cross-react with HLA-B42 antigen.Application detailsFlow cytometry: Recommended dilution: 1 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-81
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
FOLR2 (folate receptor beta) is a cell surface protein that was originally thought to be specific for placenta, but it can be also expressed in other tissues, including hematopoietic cells. Its expression is increased in malignant tissues. FOLR2 may play a role in the transport of methotrexate in synovial macrophages in rheumatoid arthritis patients. FOLR2 is a marker for macrophages generated in the presence of M-CSF (M2), including M2-like tumor-associated macrophages, which exert potent immunosuppressive functions within the tumor environment, but not GM-CSF (M1), and whose expression correlates with increased folate uptake ability.SpecificityThe mouse monoclonal antibody 3G3 recognizes N-terminal region of FoxP3, a 47-55 kDa transcription factor (intracellular antigen), which is the master regulator in the development and function of regulatory T cells.Application detailsWestern blotting: Non-reducing conditions. <br>Flow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
EM-35
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
DR3, also known as APO-3, TRAMP or TNFRSF25, is a death domain-containing receptor of TNFR family, which is expressed preferentially in peripheral blood leukocytes and in the lymphocyte-enriched tissues. Its expression has been shown to be especially up-regulated in activated T cells. DR3 participates e.g. in the removal of self-reactive T cells in the thymus. The ligand for DR3 is TL1A (TNF-like ligand 1A), which is expressed in a variety of cell types (induced by inflammatory stimuli), and can also be released as a soluble factor. The TL1A/DR3 axis has been shown to costimulate T cells to produce a wide variety of cytokines and leads to T cell differentiation towards Th1 and Th17 types.SpecificityThe mouse monoclonal antibody DBN-N-03 recognizes drebrin, an approximately 100-125 kDa intracellular regulator of actin cytoskeleton.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
JD3
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratins 10 and 13 both belong to type I family (acidic cytokeratins).SpecificityThe mouse monoclonal antibody A53-B/A2 recognizes the rod domain of cytokeratin 19 (40 kDa), an intracellular antigen constituting intermmediate cytoskeleton filaments. Cytokeratin 19 is not expressed in hepatocytes; it is often co-expressed with cytokeratin 7.Application detailsImmunohistochemistry (paraffin sections): Standard ABC technique (DAB+), recommended dilution: 10 ?g/ml (1:100), positive tissue: tonsil. Pretreatment: 0.1% pepsin in 0.1 M HCl for 30 min at room temperature or Target Retrieval Solution - High pH. The antibody DE-K13 reacts exclusively with Cytokeratin 13. <br>Immunohistochemistry (frozen sections): Use at an assay dependent dilution. <br>Western blotting: recommended dilution: 1-3 ?g/ml.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
DE-K13
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.SpecificityThe mouse monoclonal antibody DE-K13 reacts with cytokeratin 10 (56.5 kDa) and cytokeratin 13 (53 kDa). Cytokeratins are members of intermediate filaments subfamily of intracellular proteins, represented in epithelial tissues. DE-K13 recognizes only cytokeratin 13 on formalin-fixed, paraffin-embedded tissue sections.Application detailsImmunohistochemistry (paraffin sections): Recommended dilution: 10 ?g/ml; positive tissue: skin, pretreatment: trypsin/temperature 37 °C.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
VIK-10
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Cyclin D1 (PRAD1, Bcl-1) is a cytoplasmic and nuclear protein, which is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Whereas during G1 phase cyclin D1 accumulates in the nucleus, it translocates into the cytoplasm during S phase. Without growth factor-mediated stimulation, cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest.SpecificityThe antibody VIK-10 reacts with cytokeratin 10 (56.5 kDa; intracellular antigen). Cytokeratins are members of intermediate filaments subfamily represented in epithelial tissues.Application detailsImmunohistochemistry: Recommended dilution: 5-10 ?g/ml. <br>Flow cytometry: Recommended dilution: 3-12 µg/ml. Intracellular staining.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
DCS-6
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence.SpecificityThe mouse monoclonal antibody 8C11 recognizes an extracellular epitope of CD325 (N-cadherin), a transmembrane protein associated with actin cytoskeleton system, which is expressed mainly by neurons, osteoblasts, endothelial cells, and stem cells.Application detailsFlow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
IVA26
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1).SpecificityThe mouse monoclonal antibody IVA26 recognizes an extracellular epitope on MHC class I (alpha + beta2m chains) expressed in various animals.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
IVA94
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD41 (platelet glycoprotein IIb, integrin alpha IIb) is composed of two subunits (120 kDa transmembrane alpha chain and 23 kDa extracellular beta chain) and interacts with CD61 (platelet glycoprotein IIIa, integrin beta 3) in the presence of calcium to form a functional adhesive protein receptor. CD41/CD61 complex is one of the earliest markers of the megakaryocytic lineage. Upon blood vessel damage, this receptor binds to a variety of proteins including von Willebrand factor, fibrinogen, fibronectin and vitronectin, and it is involved in platelet aggregation.SpecificityThe antibody IVA94 reacts with an extracellular epitope of CD62L antigen (bovine). CD62L (L-selectin) is a 74-95 kDa single chain type I glycoprotein expressed on most peripheral blood B lymphocytes, T lymphocytes, monocytes and granulocytes; it is also present on a subset of NK cells and certain hematopoietic malignant cells.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG2
Monosan Range:
MONOSAN
Clone:
IVA30
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
SpecificityThe antibody TU-12 recognizes an epitope located within aa 345-430 of C-terminal domain of beta-tubulin in various species.Application detailsImmunohistochemistry (paraffin sections): Recommended dilution: 10 ?g/ml; standard ABC technique (DAB+), heat retrieval (microwave oven), incubation: overnight at 4°C; positive tissue: human pituitary gland.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
B31.15
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.SpecificityThe mouse monoclonal antibody EM-22 reacts with C-terminal part of beta-catenin (intracellular antigen), an 88 kDa multifunctional protein involved both in cell adhesion and in activation of transcription.Application detailsWestern blotting: Recommended dilution: 1-2 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
AE3
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. CThey are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.SpecificityThe mouse monoclonal antibody HHF35 recognizes muscle-specific alpha and gamma actin (42 kDa) in various species. This antibody stains skeletal, smooth and myocardial cells as well as myoepithelial cells and pericytes of small vessels. It is a widely used marker of muscle and muscle-derived cells.Application detailsFlow cytometry: Recommended dilution: 1-5 ?g/ml. Intracellular staining.<br>Western blotting: Recommended dilution: 1-2 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
AE1
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD89 (Fc-alpha-R) is a type I transmembrane glycoprotein serving as a receptor for IgA. Soluble CD89 is detectable in serum and retains its IgA binding capacity. For signal transduction the association with FcR gamma chain homodimers is needed. CD89 is expressed on granulocytes, monocytes, macrophages, dendritic cells and myeloid cell lines. Its expression is upregulated in presence of IgA immune complexes, stimulators (such as LPS, PMA), TNF alpha, IL1 beta or GM-CSF, and it is downregulated in presence of TGF beta and suramin. Binding of IgA-opsonized targets to CD89 leads to phagocytic and cytotoxic processes of the immunologic defense.SpecificityThe mouse monoclonal antibody A59 recognizes an extracellular epitope of CD89, a 55-100 kDa glycoprotein serving as a receptor for IgA and expressed mainly on granulocytes, monocytes and macrophages.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
A59
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD42b (GPIb alpha) composes together with GPIb beta, GPIX and GPV the GPIb-IX-V receptor complex critical in the process of platelet-rich thrombus formation by tethering the platelet to a thrombogenic surface. CD42b binds to von Willebrand factor (VWF) exposed at a site of vascular injury, as well as to thrombin, coagulation factors XI and XII, high molecular weight kininogen, TSP-1, integrin Mac-1 and P-selectin. The extracellular domain of CD42b by its interactions also contributes to metastasis. Specificity: The mouse monoclonal antibody A59 recognizes an extracellular epitope of CD89, a 55-100 kDa glycoprotein serving as a receptor for IgA and expressed mainly on granulocytes, monocytes and macrophages. Application details: Flow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
AK2
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage:
2-8°C
References 1:
Vettore S et al. Haematologica 2008, 93(11): 1743-7
References 2:
Welsh JD et al. J Thromb Haemost 2012, 10(11):2344-53
CD45RB is an of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RB is expressed e.g. in microglia and inflammatory cells.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-143
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of immunoglobulin family serving as adhesive molecule which is ubiquitously expressed in nervous system, usually as 120 kDa, 140 kDa or 180 kDa isoform, and it is also found on T cells and NK cells. Polysialic modification results in reduction of CD56-mediated cell adhesion and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity. CD56 is a widely used neuroendocrine marker with a high sensitivity for neuroendocrine tumours and ovarian granulosa cell tumours.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
MEM-188
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-241
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH. CD71 is also involved in uptake of non-transferrin bound iron.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-75
Concentration:
1 mg/ml
Storage buffer:
Tris buffered saline (TBS) solution with 15 mM sodium azide
CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation; binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-83
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD45RB is an of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RB is expressed e.g. in microglia and inflammatory cells.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-55
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD55 (decay-accelerating factor, DAF) is a GPI-anchored membrane glycoprotein that protects autologous cells from classical and alternative pathway of complement cascade. Bidirectional interactions between CD55 and CD97 are involved in T cell regulation and CD55 can still regulate complement when bound to CD97. In tumours, besides protection agains complement, CD55 promotes neoangiogenesis, tumorigenesis, invasiveness and evasion of apoptosis.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
MEM-118
Concentration:
1 mg/ml
Storage buffer:
Tris buffered saline (TBS) solution with 15 mM sodium azide
Beta2-microglobulin non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
B2M-01
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD59 (protectin) is a small (18-20 kDa) GPI-anchored ubiquitously expressed inhibitor of the membrane attack complex (MAC). It is thus the key regulator that preserves the autologous cells from terminal effector mechanism of the complement cascade. CD59 associates with C5b-8 complex and thereby counteracts appropriate formation of cytolytic pore within the plasma membrane. CD59 is also an low-affinity ligand of human CD2 and causes T cell costimulation.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
MEM-43/5
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD27 is a transmembrane 55 kDa protein of the nerve growth factor-receptor family, expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. This sCD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
LT27
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-28
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD15 (Lewis x), also known as stage specific embryonic antigen-1 (SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
MEM-158
Concentration:
1 mg/ml
Storage buffer:
Tris buffered saline (TBS) solution with 15 mM sodium azide
CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.SpecificityThe antibody BU15 reacts with an extracellular epitope of CD11c (alphaX, p150), a 150 kDa integrin expressed mainly on dendritic cells and tissue macrophages.Application detailsFlow cytometry: Recommended dilution: 1 ?g/ml; positive control: peripheral blood lymphocytes. <br> Immunoprecipitation: Positive control: granulocytes, CD11b/CD18 transfectants. <br>Immunoprecipitation with the antibody MEM-174 was successfuly performed using immuno-affinity sorbents (minicolumns). The final immunosorbent should contain 1-5 mg of antibody/1 ml of sorbent gel (e.g. agarose beads). It is recommended to stimulate positive control material by LPS 1-2 days before to achieve better expression of target antigen.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
MEM-174
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD120a / TNF R1, also known as TNFR55 or TNFRSF1A, is a 55 kDa receptor for tumor necrosis factor alpha and it is expressed in most tissues. By binding its trimeric ligand the CD120a protein forms trimers and the conformation change leads to dissociation of the inhibitory factor SODD from its intracellular death domain and in formation of signaling platform. CD120a can mediate apoptosis, and function as a regulator of inflammation. Germline mutations of the extracellular domains of this receptor were found to be associated with the autosomal dominant periodic fever syndrome. The impaired receptor clearance is thought to be a mechanism of the disease.SpecificityThe mouse monoclonal antibody TU27 recognizes an extracellular epitope of CD122 (IL-2R beta), a 70-75 kDa type I transmembrane glycoprotein constitutively expressed by NK cells and a T cell subset, and upregulated upon activation.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
H398
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD122 (IL-2/IL-15R beta) constitutes together with CD132 (common gamma chain) and with CD25 (IL-2/IL15R alpha) the intermediate (CD122+CD132) and the high affinity (CD122+CD132+CD25) IL-2 and IL-15 receptor complex. CD122 is expressed on NK cells and lymphocytes, but at low level, unless the cell is activated. The cytoplasmic part of CD122 binds to Src-family and Jak-family kinases. The biological effect of CD122 ligation depends on whether IL-2 or IL-15 is bound to the receptor complex.SpecificityThe mouse monoclonal antibody 6H6 recognizes an extracellular epitope of CD123 (interleukin 3 receptor alpha), a 60-70 kDa transmembrane protein expressed by myeloid precursors, megakaryocytes, macrophages, dendritic cells, mast cells, basophils, and some B cells. This antibody does not inhibit IL-3 binding to its receptor.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
TU27
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD123 is the alpha chain of interleukin 3 receptor (IL-3R alpha). This subunit heterodimerizes with the interleukin 3 receptor beta chain (CD131), which is shared with other receptors. CD123 interacts with IL-3 specifically, but with low affinity, and association with the beta subunit confers high affinity binding to the receptor heterodimer. Both chains are required for signaling, but receptor activation and signal transduction depend on IL-3 binding to CD123 as the initial step.SpecificityThe mouse monoclonal antibody AH9R7 recognizes an extracellular epitope of CD129 / IL-9R alpha, a 57 kDa type I transmembrane glycoprotein expressed at low levels by lymphocytes, blood cell progenitors, eosinophils, mast cells, epithelial cells, muscle cells and neurons.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
6H6
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD129 serves as the high affinity alpha subunit of IL-9 receptor. It associates with CD132, the common gamma chain shared by receptors of many different cytokines. CD129 is expressed at low levels by T and B cells, blood cell progenitors, eosinophils, mast cells, epithelial cells, muscle cells and neurons. Its signaling (through JAK/STAT pathways) results in proliferative and anti-apoptotic response, which is critical e.g. for intrathymic T cell development and survival of various cell types. The gene for CD129 is located at the pseudoautosomal regions of X and Y chromosomes and it may be related with the development of asthma.SpecificityThe mouse monoclonal antibody W6B3C1 recognizes the extracellular glycosylated epitope 1 on human CD133 (CD133/1), a 120 kDa glycoprotein of prominin family, expressed e.g. on progenitor cells. This antibody is important for identification of stem cells and tumor cells.Application detailsFlow cytometry: It is recommended to use bright fluorochromes or signal multiplying detection.
Antibody Isotype:
IgG2b kappa
Monosan Range:
MONOSAN
Clone:
AH9R7
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms – FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-154
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage:
2-8°C
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