Highly specific and reproducible dye-based qPCR Mix with blue visualisation dye for easy pipetting. Suitable for ROX-dependent and ROX-independent qPCR cyclers.
Background Info:
A universal ready-to-use 5X-concentrated solution for dye-based qPCR detection of DNA targets using EvaGreen® dsDNA intercalating dye and SYBR®/FAM detection channel. The mix is optimised for highly specific results and reduced primer-dimer formation with excellent amplification of GC-rich regions. The Mix contains dUTPs to prevent cross-contamination when used with UNG treatment, and a visible dye to ease reaction set-up. It contains an internal reference based on ROX dye and is compatible with most qPCR instruments, including those that require low and high ROX level for signal normalization.
Product Type:
Dye-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
high sensitivity with low DNA concentrations robust amplification of GC-rich targets blue visualization dye to ease pipetting reduced primer dimer formation contains dUTP to prevent cross-contamination when used in combination with UNG one qPCR mix for all cyclers (except capillary) reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start 5x EvaGreen® qPCR buffer with 12. 5 mM MgCl2: 1x PCR solution 2.5 mM MgCl2 dNTPs, including dUTP: mix allows UNG treatment to prevent carryover contamination from previous runs. IMPORTANT: UNG is not included in the 5x HOT FIREPol® EvaGreen® Supermix EvaGreen® dye Internal reference based on ROX dye GC-enhancer Blue visualisation dye
Robust and reliable hot-start Master Mix with higher fidelity and longer amplification range for more demanding reactions. Includes two tracking dyes.
Background Info:
A ready-to-use 5X-concentrated solution for more demanding endpoint PCR assays. In addition to the hot-start Taq polymerase HOT FIREPol® this master mix contains a proofreading enzyme which offers enhanced fidelity and performance. The mix has regular and Ready-to-Load version and different MgCl? options for easier optimization. Ready-to-Load mix ncludes loading dyes for direct loading to gel.
Product Type:
Endpoint PCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
increased yield, sensitivity and specificity up to 5x higher fidelity suitable for templates up to 5 kb reduced primer dimer formation Ready-to-Load mix available reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start Proofreading enzyme: to enhance fidelity 5x Blend Master Mix Buffer with 7. 5 mM MgCl2: 1x PCR solution 1.5 mM MgCl2 1 mM dNTPs of each: 1x PCR solution 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP Bovine serum albumine (BSA) to enhance PCR reaction Blue dye: migration equivalent to 3.5-4.5 kb DNA fragment Yellow dye: migration rate in excess of primers in 1% agarose gel: <35-45 bp Compound that increases sample density for direct loading
Hot-start Master Mix designed to provide highly specific high-yield amplification of GC-rich templates.
Background Info:
A ready-to-use 5X-concentrated solution for endpoint PCR assays, optimised for GC-rich templates. It contains a hot-start Taq DNA polymerase HOT FIREPol® and allows amplification of up to 5 kb DNA templates. Separate tubes of 25 mM MgCl? and an additive for difficult templates (100% DMSO) are included with the mix.
Product Type:
Endpoint PCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
excellent amplification with templates up to 79% GC content suitable for templates up to 5 kb vials of 100% DMSO and 25 mM MgCl? enable flexibility in reaction optimization reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start 5x HOT FIREPol® GC Buffer with 7.5 mM MgCl2: 1x PCR solution 1.5 mM MgCl2 dNTPs: dATP, dCTP, dGTP and dTTP Bovine serum albumin (BSA) to enhance PCR reaction In separate vials: 100% DMSO is included in the kit in a separate vial. DMSO is recommended as a PCR additive for templates with high GC content. In some cases, DMSO is also required to relax secondary structures. 25 mM MgCl2 for further optimization
Vials included in the kit:
HOT FIREPol® GC Master Mix 1x20 ml | 5000 rxn 25 mM MgCl2 20x0.5 ml DMSO 100% 1x10 ml
Robust and reliable hot-start Master Mix with higher fidelity and longer amplification range for more demanding reactions. Includes two tracking dyes.
Background Info:
A ready-to-use 5X-concentrated solution for more demanding endpoint PCR assays. In addition to the hot-start Taq polymerase HOT FIREPol® this master mix contains a proofreading enzyme which offers enhanced fidelity and performance. The mix has regular and Ready-to-Load version and different MgCl? options for easier optimization. Ready-to-Load mix ncludes loading dyes for direct loading to gel.
Product Type:
Endpoint PCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
increased yield, sensitivity and specificity up to 5x higher fidelity suitable for templates up to 5 kb reduced primer dimer formation Ready-to-Load mix available reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start Proofreading enzyme: to enhance fidelity 5x Blend Master Mix Buffer with 12. 5 mM MgCl2: 1x PCR solution 2.5 mM MgCl2 1 mM dNTPs of each 1x PCR solution 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP Bovine serum albumine (BSA) to enhance PCR reaction Blue dye: migration equivalent to 3.5-4.5 kb DNA fragment Yellow dye: migration rate in excess of primers in 1% agarose gel: <35-45 bp Compound that increases sample density for direct loading
Robust and reliable hot-start Master Mix with higher fidelity and longer amplification range for more demanding reactions. Includes two tracking dyes.
Background Info:
A ready-to-use 5X-concentrated solution for more demanding endpoint PCR assays. In addition to the hot-start Taq polymerase HOT FIREPol® this master mix contains a proofreading enzyme which offers enhanced fidelity and performance. The mix has regular and Ready-to-Load version and different MgCl? options for easier optimization. Ready-to-Load mix ncludes loading dyes for direct loading to gel.
Product Type:
Endpoint PCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
increased yield, sensitivity and specificity up to 5x higher fidelity suitable for templates up to 5 kb reduced primer dimer formation Ready-to-Load mix available reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start Proofreading enzyme: to enhance fidelity 5x Blend Master Mix Buffer with 10 mM MgCl2: 1x PCR solution 2 mM MgCl2 1 mM dNTPs of each: 1x PCR solution 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP Bovine serum albumin (BSA) to enhance PCR reaction Blue dye: migration equivalent to 3.5-4.5 kb DNA fragment Yellow dye: migration rate in excess of primers in 1% agarose gel: <35-45 bp Compound that increases sample density for direct loading
Probe-based qPCR Mix optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays
Background Info:
A ready-to-use 5X-concentrated solution for singleplex and multiplex endpoint PCR assays. It contains a hot-start Taq DNA polymerase HOT FIREPol® and allows amplification of up to 5 kb DNA templates. The mix has two versions - regular and Ready-to-Load mix which includes loading dyes for direct loading to gel.
Product Type:
Endpoint PCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
sensitive detection of up to 18 targets per reaction suitable for templates up to 5 kb increased sensitivity and yield reduced primer dimer formation Ready-to-Load mix available reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase 5x Multiplex Buffer with 10 mM MgCl2 (1x PCR solution 2 mM MgCl2) dNTPs BSA Compound that increases sample density for direct loading (loading dyes not included)
Sensitive qPCR Mix designed for effective quantification of DNA targets with probe-based chemistry.
Background Info:
A cost-effective ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 2 targets in one reaction. It has three versions to match different instruments. ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization. Capillary-mix is optimised for capillary based systems.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cost-effective solution for less demanding assays suitable for assays with 1-2 targets high specificity and sensitivity compatible with most cyclers different product versions: no ROX, ROX, capillary reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
5x Probe qPCR buffer with 15 mM MgCl2: 1x PCR solution 3 mM MgCl2
Sensitive qPCR Mix designed for effective quantification of DNA targets with probe-based chemistry.
Background Info:
A cost-effective ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 2 targets in one reaction. It has three versions to match different instruments. ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization. Capillary-mix is optimised for capillary based systems.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cost-effective solution for less demanding assays suitable for assays with 1-2 targets high specificity and sensitivity compatible with most cyclers different product versions: no ROX, ROX, capillary reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
5x Probe qPCR buffer with 15 mM MgCl2: 1x PCR solution 3 mM MgCl2
Sensitive qPCR Mix designed for effective quantification of DNA targets with probe-based chemistry.
Background Info:
A cost-effective ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 2 targets in one reaction. It has three versions to match different instruments. ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization. Capillary-mix is optimised for capillary based systems.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cost-effective solution for less demanding assays suitable for assays with 1-2 targets high specificity and sensitivity compatible with most cyclers different product versions: no ROX, ROX, capillary reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
5x Probe qPCR buffer with 15 mM MgCl2: 1x PCR solution 3 mM MgCl2
A ready-to-use 5X-concentrated solution for High Resolution Melt (HRM) analysis of DNA targets using EvaGreen® dsDNA intercalating dye and SYBR®/FAM detection. The mix has two versions to match different instruments. The ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization.
Product Type:
Dye-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
excellent resolution in HRM assays allows detection of DNA sequence variations contains sensitive EvaGreen® dye compatible with most cyclers different product versions: no ROX, ROX reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start 5x EvaGreen® qPCR buffer with 12. 5 mM MgCl2, 1x PCR solution 2.5 mM MgCl2 dNTPs: dATP, dCTP, dGTP and dTTP EvaGreen® dye Bovine serum albumin (BSA) to enhance qPCR reaction
Highly sensitive dye-based qPCR Mix with improved detection of low-copy targets. Compatible with most qPCR platforms.
Background Info:
A highly sensitive ready-to-use 5X reaction mix for dye-based qPCR detection of DNA targets using SolisGreen® dsDNA intercalating dye and SYBR®/FAM detection channel. The mix has excellent performance with low template amounts. It contains contains an internal reference based on ROX dye and is compatible with qPCR instruments that require low ROX level for signal normalization. SolisGreen® qPCR mixes are suitable for commercial and diagnostic applications and require no additional licensing.
Product Type:
Dye-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
enhanced performance with low DNA concentrations bright and sensitive SolisGreen® dye suitable for commercial applications one qPCR mix for all cyclers, except high ROX reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start 5x qPCR buffer with 12.5 mM MgCl2: 1x PCR solution 2.5 mM MgCl2 dNTPs: dATP, dCTP, dGTP and dTTP SolisGreen® dye Bovine serum albumin (BSA) to enhance PCR reaction Internal reference based on ROX dye
High performing probe-based qPCR Mix for AT-rich, regular and GC-rich templates.
Background Info:
A universal ready-to-use 5X reaction mix for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 2 targets in one reaction with excellent amplification of GC-rich regions. The mix contains dUTPs to prevent carry-over contamination when used with UNG treatment, and an internal reference based on ROX dye and is compatible with most qPCR instruments, including those that require low and high ROX level for signal normalization. The mix is not compatible with Probes detected in ROX/JUN/Texas Red channel.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
suitable for assays with 1-2 targets high specificity and sensitivity superior results with templates with up to 75% GC content contains dUTP to prevent cross-contamination when used with UNG one qPCR mix for all cyclers (except capillary) reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start
5x Probe Universal qPCR buffer with 15 mM MgCl2: 1x PCR solution 3 mM MgCl2
dNTPs including dUTP: Mix allows UNG treatment to prevent carryover contamination from previous runs. IMPORTANT: UNG is not included in the 5x HOT FIREPol® Probe Universal qPCR Mix
Reference dye based on ROX: For multiplex application - if ROX dye is used as one of the fluorophores, the internal reference might interfere with the signal.
In separate vial
100% DMSO is included in the kit in a separate vial. DMSO is recommended as a PCR additive for templates with high GC content. In some cases, DMSO is also required to relax secondary structures.
Vials included in the kit:
HOT FIREPol® Probe Universal qPCR Mix 1x20 ml | 5000 rxn DMSO 100% 1 10 ml
Ready-to-use solution for simple dye-based qPCR assays, incorporating EvaGreen® dye. Available for ROX, no ROX and cappillay cyclers.
Background Info:
A cost-effective ready-to-use 5X-concentrated solution for dye-based qPCR detection of DNA targets using EvaGreen® dsDNA intercalating dye and SYBR®/FAM detection channel. The mix has three versions to match different instruments. The ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization. The capillary-mix is optimised for capillary based systems.
Product Type:
Dye-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cost-effective solution for less demanding assays suitable for most dye-based applications high sensitivity and specificity excellent efficiency compatible with most cyclers different product versions: no ROX, ROX, capillary reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start 5x EvaGreen® qPCR buffer with 12. 5 mM MgCl2: 1x PCR solution 2.5 mM MgCl2 dNTPs: dATP, dCTP, dGTP and dTTP EvaGreen® dye Internal reference based on ROX dye
Ready-to-use solution for simple dye-based qPCR assays, incorporating EvaGreen® dye. Available for ROX, no ROX and cappillay cyclers.
Background Info:
A cost-effective ready-to-use 5X-concentrated solution for dye-based qPCR detection of DNA targets using EvaGreen® dsDNA intercalating dye and SYBR®/FAM detection channel. The mix has three versions to match different instruments. The ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization. The capillary-mix is optimised for capillary based systems.
Product Type:
Dye-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cost-effective solution for less demanding assays suitable for most dye-based applications high sensitivity and specificity excellent efficiency compatible with most cyclers different product versions: no ROX, ROX, capillary reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start 5x EvaGreen® qPCR buffer with 12. 5 mM MgCl2: 1x PCR solution 2.5 mM MgCl2 dNTPs: dATP, dCTP, dGTP and dTTP EvaGreen® dye
A ready-to-use 5X-concentrated solution for High Resolution Melt (HRM) analysis of DNA targets using EvaGreen® dsDNA intercalating dye and SYBR®/FAM detection. The mix has two versions to match different instruments. The ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization.
Product Type:
Dye-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
excellent resolution in HRM assays allows detection of DNA sequence variations contains sensitive EvaGreen® dye compatible with most cyclers different product versions: no ROX, ROX reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start 5x EvaGreen® qPCR buffer with 12. 5 mM MgCl2: 1x PCR solution 2.5 mM MgCl2 dNTPs: dATP, dCTP, dGTP and dTTP EvaGreen® dye ROX reference dye Bovine serum albumin (BSA) to enhance qPCR reaction
Ready-to-use solution for simple dye-based qPCR assays, incorporating EvaGreen® dye. Available for ROX, no ROX and cappillay cyclers.
Background Info:
A cost-effective ready-to-use 5X-concentrated solution for dye-based qPCR detection of DNA targets using EvaGreen® dsDNA intercalating dye and SYBR®/FAM detection channel. The mix has three versions to match different instruments. The ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization. The capillary-mix is optimised for capillary based systems.
Product Type:
Dye-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cost-effective solution for less demanding assays suitable for most dye-based applications high sensitivity and specificity excellent efficiency compatible with most cyclers different product versions: no ROX, ROX, capillary reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start 5x EvaGreen® qPCR buffer with 12.5 mM MgCl2: 1x PCR solution 2.5 mM MgCl2 dNTPs: dATP, dCTP, dGTP and dTTP EvaGreen® dye Bovine serum albumine (BSA) to enhance qPCR reaction
Highly specific and reproducible dye-based qPCR Mix with blue visualisation dye for easy pipetting. Suitable for ROX-dependent and ROX-independent qPCR cyclers.
Background Info:
A universal ready-to-use 5X-concentrated solution for dye-based qPCR detection of DNA targets using EvaGreen® dsDNA intercalating dye and SYBR®/FAM detection channel. The mix is optimised for highly specific results and reduced primer-dimer formation with excellent amplification of GC-rich regions. The Mix contains dUTPs to prevent cross-contamination when used with UNG treatment, and a visible dye to ease reaction set-up. It contains an internal reference based on ROX dye and is compatible with most qPCR instruments, including those that require low and high ROX level for signal normalization.
Product Type:
Dye-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
high sensitivity with low DNA concentrations robust amplification of GC-rich targets blue visualization dye to ease pipetting reduced primer dimer formation contains dUTP to prevent cross-contamination when used in combination with UNG one qPCR mix for all cyclers (except capillary) reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start 5x EvaGreen® qPCR buffer with 12. 5 mM MgCl2: 1x PCR solution 2.5 mM MgCl2 dNTPs, including dUTP: mix allows UNG treatment to prevent carryover contamination from previous runs. IMPORTANT: UNG is not included in the 5x HOT FIREPol® EvaGreen® Supermix EvaGreen® dye Internal reference based on ROX dye GC-enhancer Blue visualisation dye
Ultra-fast endpoint PCR Master Mix that includes novel in-silico designed SolisFAST® DNA Polymerase for robust and accurate target detection.
Background Info:
A ready-to-use 5X-concentrated solution for fast and ultra-fast singleplex and multiplex endpoint PCR assays. It contains a novel SolisFAST® DNA Polymerase with fast hot-start (30 sec to 3 min) and 2-4 times faster extension rates (15-30 sec/kb) compared to the wild-type Taq DNA polymerase (60 sec/kb) as well as HOT FIREPol® and FIREPol® DNA polymerases. The mix allows amplification of up to 5 kb DNA templates and has two versions - regular and Ready-to-Load mix which includes loading dyes for direct loading to gel.
Product Type:
Endpoint PCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
PCR results in 20 minutes fast hot-start (30 sec-3 min) fast amplification (15-30 sec/kb) sensitive detection of up to 18 targets per reaction suitable for templates up to 5 kb reliable results in Sanger sequencing applications Ready-to-Load mix available for direct loading to gel UNG-mix available to prevent carry-over contamination reaction set-up and shipment without ice
Mix/Kit Components:
SolisFAST® DNA Polymerase: In silico designed analogue of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild-type Taq DNA polymerase. PCR buffer: Includes 17.5 mM MgCl2 (1x PCR solution 3.5 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dTTP) and additives that maximize efficiency of PCR
Ultra-fast endpoint PCR Master Mix that includes novel in-silico designed SolisFAST® DNA Polymerase for robust and accurate target detection. Suitable for UNG treatment to prevent cross-contaminaton
Background Info:
A ready-to-use 5X-concentrated solution for fast and ultra-fast singleplex and multiplex endpoint PCR assays. It contains a novel SolisFAST® DNA Polymerase with fast hot-start (30 sec to 3 min) and 2-4 times faster extension rates (15-30 sec/kb) compared to the wild-type Taq DNA polymerase (60 sec/kb) as well as HOT FIREPol® and FIREPol® DNA polymerases. The mix allows amplification of up to 5 kb DNA templates and has two versions - regular and Ready-to-Load mix which includes loading dyes for direct loading to gel.
Product Type:
Endpoint PCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
PCR results in 20 minutes fast hot-start (30 sec-3 min) fast amplification (15-30 sec/kb) sensitive detection of up to 18 targets per reaction suitable for templates up to 5 kb reliable results in Sanger sequencing applications Ready-to-Load mix available for direct loading to gel UNG-mix available to prevent carry-over contamination reaction set-up and shipment without ice
Mix/Kit Components:
SolisFAST® DNA Polymerase: In silico designed analogue of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild-type Taq DNA polymerase PCR Buffer: Includes 17.5 mM MgCl2 (1x PCR solution 3.5 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dUTP), UNG, additives that maximize efficiency of PCR
Ultra-fast endpoint PCR Master Mix that includes novel in-silico designed SolisFAST® DNA Polymerase for robust and accurate target detection. Includes two tracking dyes.
Background Info:
A ready-to-use 5X-concentrated solution for fast and ultra-fast singleplex and multiplex endpoint PCR assays. It contains a novel SolisFAST® DNA Polymerase with fast hot-start (30 sec to 3 min) and 2-4 times faster extension rates (15-30 sec/kb) compared to the wild-type Taq DNA polymerase (60 sec/kb) as well as HOT FIREPol® and FIREPol® DNA polymerases. The mix allows amplification of up to 5 kb DNA templates and has two versions - regular and Ready-to-Load mix which includes loading dyes for direct loading to gel.
Product Type:
Endpoint PCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
PCR results in 20 minutes fast hot-start (30 sec-3 min) fast amplification (15-30 sec/kb) sensitive detection of up to 18 targets per reaction suitable for templates up to 5 kb reliable results in Sanger sequencing applications Ready-to-Load mix available for direct loading to gel UNG-mix available to prevent carry-over contamination reaction set-up and shipment without ice
Mix/Kit Components:
SolisFAST® DNA Polymerase: In silico designed analogue of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild-type Taq DNA polymerase. PCR buffer: Includes 17.5 mM MgCl2 (1x PCR solution 3.5 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dTTP), additives that maximize efficiency of PCR Green loading dye: Mix of blue and yellow dye to allow tracking of DNA during electrophoresis. The mix is not recommended for use in applications where spectrophotometric measurements are necessary (e.g. Sanger sequencing). Migration speed: blue dye TBE: ~3.5 kb, TAE: ~4.5 kb; yellow dye TBE: ~35 bp, TAE: ~45 bp).
Ultra fast endpoint PCR Master Mix that includes novel in-silico designed SolisFAST® DNA Polymerase for robust and accurate target detection. Suitable for UNG treatment to prevent cross-contamination. Includes two tracking dyes.
Background Info:
A ready-to-use 5X-concentrated solution for fast and ultra-fast singleplex and multiplex endpoint PCR assays. It contains a novel SolisFAST® DNA Polymerase with fast hot-start (30 sec to 3 min) and 2-4 times faster extension rates (15-30 sec/kb) compared to the wild-type Taq DNA polymerase (60 sec/kb) as well as HOT FIREPol® and FIREPol® DNA polymerases. The mix allows amplification of up to 5 kb DNA templates and has two versions - regular and Ready-to-Load mix which includes loading dyes for direct loading to gel.
Product Type:
Endpoint PCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
PCR results in 20 minutes fast hot-start (30 sec-3 min) fast amplification (15-30 sec/kb) sensitive detection of up to 18 targets per reaction suitable for templates up to 5 kb reliable results in Sanger sequencing applications Ready-to-Load mix available for direct loading to gel UNG-mix available to prevent carry-over contamination reaction set-up and shipment without ice
Mix/Kit Components:
SolisFAST® DNA Polymerase: In silico designed analogue of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild-type Taq DNA polymerase. PCR buffer: Includes 17.5 mM MgCl2 (1x PCR solution 3.5 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dUTP), UNG, additives that maximize efficiency of PCR Green loading dye: Mix of blue and yellow dye to allow tracking of DNA during electrophoresis. The mix is not recommended for use in applications where spectrophotometric measurements are necessary (e.g. Sanger sequencing). Migration speed: blue dye TBE: ~3.5 kb, TAE: ~4.5 kb; yellow dye TBE: ~35 bp, TAE: ~45 bp).
Probe-based qPCR Mix optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays
Background Info:
A ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 4 targets in one reaction with enhanced amplification of GC-rich regions. It contains dUTPs to prevent carry-over contamination when used with UNG treatment, and has three versions to match different instruments and assay requirements. ROX-mix is compatible with most qPCR instruments, including those that require low and high ROX level for signal normalization, Purple-mix is compatible with instruments that use Mustang Purple for signal normalisation.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
analyze 1-4 targets in 1 reaction high specificity and sensitivity robust amplification of GC-rich targets contains dUTP to prevent carry-over contamination when used in combination with UNG compatible with most cyclers (see table on page 17) different product versions: no ROX, ROX, Purple reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
5x Multiplex qPCR buffer with 15 mM MgCl2: 1x PCR solution 3mM MgCl2
dNTPs including dUTP: the mix allows UNG treatment to prevent carryover contamination from previous runs. IMPORTANT: UNG is not included in the 5x HOT FIREPol® Multiplex qPCR Mix
Ultra-fast probe-based qPCR Master Mix that includes novel in-silico designed SolisFAST® DNA Polymerase for robust and accurate target quantification.
Background Info:
Fast and sensitive ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix contains a novel SolisFAST® DNA Polymerase with fast hot-start and 2-4 times faster extension rates (15-30 sec/kb) compared to the wild-type Taq DNA polymerase (60 sec/kb) as well as HOT FIREPol® and FIREPol® DNA polymerases, and is optimised for sensitive detection of up to 5 targets in one reaction. It has three versions to match different instruments and assay requirements. ROX-mix is compatible with qPCR instruments that require low and high ROX level for signal normalization, Purple-mix is compatible with instruments that use Mustang Purple for signal normalisation. dUTP and UNG containing mixes are available upon request to prevent carry-over contamination.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
qPCR results 2x faster fast hot-start (30 sec-3 min) fast amplification (15-30 sec/kb) analyze 1-5 targets in 1 reaction mix with dUTP and UNG available to prevent carry-over contamination compatible with most cyclers different product versions: no ROX, ROX, Purple reaction set-up and shipment without ice
Mix/Kit Components:
SolisFAST® DNA Polymerase: In silico- designed analogue of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild-type Taq DNA polymerase. qPCR buffer: Includes 15 mM MgCl2 (1x PCR solution 3 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dTTP), additives that maximize efficiency of PCR
Ultra fast probe-based qPCR Master Mix that includes novel in-silico designed SolisFAST® DNA Polymerase for robust and accurate target quantification. Suitable for UNG treatment to prevent cross-contamination.
Background Info:
Fast and sensitive ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix contains a novel SolisFAST® DNA Polymerase with fast hot-start and 2-4 times faster extension rates (15-30 sec/kb) compared to the wild-type Taq DNA polymerase (60 sec/kb) as well as HOT FIREPol® and FIREPol® DNA polymerases, and is optimised for sensitive detection of up to 5 targets in one reaction. It has three versions to match different instruments and assay requirements. ROX-mix is compatible with qPCR instruments that require low and high ROX level for signal normalization, Purple-mix is compatible with instruments that use Mustang Purple for signal normalisation. dUTP and UNG containing mixes are available upon request to prevent carry-over contamination.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
qPCR results 2x faster fast hot-start (30 sec-3 min) fast amplification (15-30 sec/kb) analyze 1-5 targets in 1 reaction mix with dUTP and UNG available to prevent carry-over contamination compatible with most cyclers different product versions: no ROX, ROX, Purple reaction set-up and shipment without ice
Mix/Kit Components:
SolisFAST® DNA Polymerase: In silico- designed analog of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start, and faster extension rates compared to the wild-type Taq DNA polymerase. qPCR buffer: Includes 16.5 mM MgCl2 - 1x PCR solution 3.3 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dUTP), UNG and additives that maximize efficiency of PCR Reference dye: Purple dye is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR
Probe-based qPCR Mix optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays
Background Info:
A ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 4 targets in one reaction with enhanced amplification of GC-rich regions. It contains dUTPs to prevent carry-over contamination when used with UNG treatment, and has three versions to match different instruments and assay requirements. ROX-mix is compatible with most qPCR instruments, including those that require low and high ROX level for signal normalization, Purple-mix is compatible with instruments that use Mustang Purple for signal normalisation.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
analyze 1-4 targets in 1 reaction high specificity and sensitivity robust amplification of GC-rich targets contains dUTP to prevent carry-over contamination when used in combination with UNG compatible with most cyclers (see table on page 17) different product versions: no ROX, ROX, Purple reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
5x Multiplex qPCR buffer with 15 mM MgCl2: 1x PCR solution 3mM MgCl2
dNTPs including dUTP: the mix allows UNG treatment to prevent carryover contamination from previous runs. IMPORTANT: UNG is not included in the 5x HOT FIREPol® Multiplex qPCR Mix
Purple dye: If Cy5 or similar dye is used as one of the fluorophores, Purple passive reference dye might interfere with the signal. A version without passive reference or with ROX reference dye is available
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