GLDP (Glycine decarboxylase P protein) catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor. Is involved in response to cadmium ion. Alternative names: AtGLDP1, AtGLDP2, Glycine dehydrogenase (aminomethyl-transferring) 1, Glycine dehydrogenase (aminomethyl-transferring) 2, Glycine cleavage system P protein 1, Glycine cleavage system P protein 2, Glycine decarboxylase 1, Glycine decarboxylase 2, Glycine decarboxylase P-protein 1, Glycine decarboxylase P-protein 2.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
So far only leaf tissue has been used for immunolocalization and western blot experiments
Application Details:
1:50 (IL), 1 : 5000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l, of sterile water
Molecular Weight:
113 kDa
Not reactive in:
Tecticornia spp., Salsola soda
Selected references:
Khoshravesh et al. (2020). The Evolutionary Origin of C4 Photosynthesis in the Grass Subtribe Neurachninae.Plant Physiol. 2020 Jan;182(1):566-583. doi: 10.1104/pp.19.00925.
ClpR4 (ATP-dependent Clp protease proteolytic subunit-related protein 4, chloroplastic) is involved in plastid protein homeostasis. Alternative names: ClpP4, Protein HAPPY ON NORFLURAZON 5.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica oleracea, Cajanus cajan, Capsella rubella, Coffea canephora, Cucumis sativus, Erythranthe guttata, Eutrema salsugineum, Genlisea aurea, Glycine max, Jatropha curcas, Medicago truncatula, Morus notabilis, Populus trichocarpa, Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Vitis viniferaSpecies of your interest not listed? Contact us
Immunogen:
BSA-conjugated peptide derived from ClpR4 of Arabidopsis thaliana, TAIR: AT4G17040, UniProt: Q8LB10
For western blot detection image refer to the article below
Application Details:
1 : 4000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
33,4 | 24,5 kDa
Not reactive in:
Zea mays
Selected references:
Sj gren et al. (2004). Inactivation of the clpC1 gene encoding a chloroplast Hsp100 molecular chaperone causes growth retardation, leaf chlorosis, lower photosynthetic activity, and a specific reduction in photosystem content. Plant Physiol. 2004 Dec;136(4):4114-26. Epub 2004 Nov 24.
EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase, chloroplastic) is an enzyme (EC:2.5.1.19)which is involved in glyphosate metabolic process. Localized in chloroplast stroma. Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate. Alternative name: 5-enolpyruvylshikimate-3-phosphate synthase.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Amaranthus palmeri
Expected Species:
Asimina triloba, Erigeron annuus, Chlamydomonas reinhardtii, Genlisea aurea, Gossypium raimondii, Lolium rigidum, Musa acuminata, Nannochloropsis gaditana, Nicotiana tabacum, Nicotiana sylvestris, Oryza sativa subsp. indica, Plantago lanceolata, Sorghum halepense, Zea mays, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide, derived from Amaranthus palmeri EPSP synthase, UniProt: M1K439
Fern ndez-Escalada et al. (2016). Characterization of the Amaranthus palmeri Physiological Response to Glyphosate in Susceptible and Resistant Populations. J Agric Food Chem. 64 (1): 95-106. doi: 10.1021/acs.jafc.5b04916.
Double-stranded RNA-binding protein 4 (DRB4) plays a role in RNA-mediated post-transcriptional gene silencing (PTGS). It assists DCL4 during biogenesis of trans-acting small interferring RNAs (ta-siRNAs) and is necessary for DCL4 activity. DRB4 is also involved in RNA silencing of RNA and DNA viruses.Alternative name: dsRNA-binding protein 4, DBR4
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica napus,Brassica oleracea, Camelina sativa, Capsella rubella, Eutrema salsugineum Species of your interest not listed? Contact us
FNRL | Ferredoxin-NADP+ oxidoreductase-like (FNRs; EC:1.18.1.2) is a member of the plant-type FNR family. It contains a two-domain scaffold that forms the basis of an extended superfamily of flavin adenine dinucleotide (FAD) dependent oxidoreductases. FNRL is soluble protein in chloroplast stroma.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica cretica, Capsella rubella, Eutrema salsugineum, Noccaea caerulescens Species of your interest not listed? Contact us
SUMO3 (Small ubiquitin-related modifier 3) is a small polypeptide that becomes covalently attached to various intracellular proteins leading to their post-translational modification.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C (short tem, months) or at -80°C(long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Immunogen:
Full length SUMO3 of Arabidopsis thaliana, UniProt: Q9FLP5, TAIR: At5g55170
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Kurepa et al. (2003). The small ubiquitin-like modifier (SUMO) protein modification system in Arabidopsis. Accumulation of SUMO1 and -2 conjugates is increased by stress. J Biol Chem. 2003 Feb 28;278(9):6862-72.
Cysteine protease Cp2 shows proteolytic activity and is involved in programmed cell death in plant during pathogen infection.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Carica papaya
Expected Species:
Cajanus cajan, Cicer arietinum, Cucumis sativus, Glycine soja, Gossypium hirsutum, Medicago truncatula, Phaseolus vulgaris, Trifolium pratense, Vicia sativa, Vigna radiata var. radiata Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from cysteine protease sequence of Carica papaya UniProt: H6USN1
Calcium-transporting ATPase 1, endoplasmic reticulum-type is a magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the endoplasmic reticulum lumen. Also regulate manganese ion homeostasis by pumping it into endomembrane compartments. The enzyme can also transport zinc. Alternative names: ACA3, ARABIDOPSIS THALIANA ER-TYPE CA2+-ATPASE 1, ATECA1, ECA1, ER-TYPE CA2+-ATPASE 1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Lyophilized antibody can be stored at -20 °C for up to 3 years. Re-constituted antibody can be stored at 4°Cfor several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide derived from Arabidopsis thaliana ECA1 sequence, cytosolic loop, between TM4 and TM5, UniProt:P92939, TAIR: At1g07810
The protein shows tissue specific expression, shown on a blot included in Wu et al. (2000). Lowest amounts of ECA1 are found in siliques.
Application Details:
1 : 10 000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l, of sterile water
Molecular Weight:
116,3 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Wu et al. (2000). An endoplasmic reticulum-bound Ca(2+)/Mn(2+) pump, ECA1, supports plant growth and confers tolerance to Mn(2+) stress. Plant Physiol. 2002 Sep;130(1):128-37.Liang et al. (1997). ECA1 complements yeast mutants defective in Ca2+ pumps and encodes an endoplasmic reticulum-type Ca2+-ATPase in Arabidopsis thaliana. Proc Natl Acad Sci U S A. 1997 Aug 5;94(16):8579-84.
ACA2 (Calcium-transporting ATPase 2) is a magnesium-dependent enzyme ((EC:3.6.3.8), which catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the endoplasmic reticulum. Alternative name: Ca(2+)-ATPase isoform 2
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Immunogen:
GST-fusion of ACA2 peptide, purified by SDS-PAGE of Arabidopsis thaliana ACA2 protein sequence, UniProt: O81108, TAIR: AT4G37640
ACA2 can be found in a good levels in: roots and flowers while the levels in leaves and siliques are very low. Harper et al. 1998.This antibody is recognizing both full length and trunctated forms.
Application Details:
1 : 10 000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l, of sterile water
Molecular Weight:
110 | 110 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Hwang et al. (2000). Calmodulin activation of an endoplasmic reticulum-located calcium pump involves an interaction with the N-terminal autoinhibitory domain.Plant Physiol. 2000 Jan;122(1):157-68.Hwang et al. (2000). Calmodulin activation of an endoplasmic reticulum-located calcium pump involves an interaction with the N-terminal autoinhibitory domain.Plant Physiol. 2000 Jan;122(1):157-68.Harper et al. (1998). A novel calmodulin-regulated Ca2+-ATPase (ACA2) from Arabidopsis with an N-terminal autoinhibitory domain. J Biol Chem. 1998 Jan 9;273(2):1099-106. (this paper contains a blot of tissue specific expression of ACA2).Harper et al. (1998). A novel calmodulin-regulated Ca2+-ATPase (ACA2) from Arabidopsis with an N-terminal autoinhibitory domain. J Biol Chem. 1998 Jan 9;273(2):1099-106. (this paper contains a blot of tissue specific expression of ACA2)
ATG16 (Autophagy-related protein 16) may play a role in autophagy process.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C (short tem, months) or at -80°C(long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Immunogen:
N-terminal part of of ATG16 of Arabidopsis thaliana UniProt: Q6NNP0, TAIR: At5g50230
endo-beta 1,4 glucanase (hyperthermophilic enzyme) is an enzyme with cellulase activity involved in polysaccharide catabolic process.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Thermotoga neapolitana
Expected Species:
Arabidopsis thaliana Species of your interest not listed? Contact us
Immunogen:
His-tagged, recombinant from endo-beta 1,4 glucanase (hyperthermophilic enzyme) of Thermotoga neapolitana, overexpressed in E.coli, UniProt: B9K8J9
Gamma CAH (Gamma Carbonic anhydrase) proteins are localized in the inner mitochondrial membrane. They are in a range of 25-30 kDa.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Oryza sativa
Expected Species:
Arachis duranensis, Brachypodium distachyon, Brassica oleracea, Brassica rapa, Camelina sativa, Capsella rubella, Citrus sinensis, Daucus carota, Erythranthe guttatus, Fragaria vesca subsp. vesca, Gossypium hirsutum, Jatropha curcas, Juglans regia, Lupinus angustifolius, Malus x domestica, Medicago truncatula, Nelumbo nucifera, Oryza brachyantha, Populus euphratica, Prunus mume, Pyrus x bretschneideri, Raphanus sativus, Sesamum indicum, Setaria italica, Solanum tuberosum, Tarenaya hassleriana, Theobroma cacao, Triticum aestivum, Zea mays, Zostera muelleri Species of your interest not listed? Contact us
Chen et al. (2019). Composition of Mitochondrial Complex I during the Critical Node of Seed Aging in Oryza sativa. Journal of Plant Physiology Volume 236, May 2019, Pages 7-14.K hn et al. (2015). Complete Mitochondrial Complex I Deficiency Induces an Up-Regulation of Respiratory Fluxes That Is Abolished by Traces of Functional Complex I. Plant Physiol. 2015 Aug;168(4):1537-49. doi: 10.1104/pp.15.00589. Epub 2015 Jul 1.
ATG13a (Autophagy-related protein 13a) is involved in autophagy in a nutritional condition dependent manner. The ATG1-ATG13 protein kinase complex regulates downstream events required for autophagosome enclosure and/or vacuolar delivery. Alternative names: AtAPG13a.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C (short tem, months) or at -80°C(long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Noccaea caerulescensSpecies of your interest not listed? Contact us
Immunogen:
Full length, recombinant ATG13a of Arabidopsis thaliana protein sequence UniProt: F4IXZ6, TAIR: At3g49590 with N-terminal HIS-tag.
Antibody may also recognise ATG13b; multiple bands observed due to phosphorylation
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l, of sterile water
Molecular Weight:
68,4 | 70-80 kDa
Not reactive in:
Oryza sativa
Selected references:
Suttangkakul et al. (2011). The ATG1/ATG13 protein kinase complex is both a regulator and a target of autophagic recycling in Arabidopsis. Plant Cell. 2011 Oct;23(10):3761-79. doi: 10.1105/tpc.111.090993.
ATG12b (Autophagy-related protein 12b) is an Ubiquitin-like protein involved in cytoplasm to vacuole transport (Cvt) and autophagy vesicles formation. Alternative names: Ubiquitin-like protein ATG12B, APG12-like protein b, AtAPG12b.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C (short tem, months) or at -80°C(long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Immunogen:
Recombinant, full length ATG12b ofArabidopsis thaliana protein sequence UniProt: Q9LVK3-1, TAIR: At3g13970
This antibody may also recognise ATG12a; in wild-type, ATG12 is also conjugated to ATG5
Application Details:
1 : 3000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l, of sterile water
Molecular Weight:
10,4 | 12 and 50 kDa in wilde type plants and 12 kDa in some autophagy deficient mutants
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Chung et al. (2010). ATG8 lipidation and ATG8-mediated autophagy in Arabidopsis require ATG12 expressed from the differentially controlled ATG12A AND ATG12B loci. Plant J. 2010 May;62(3):483-93. doi: 10.1111/j.1365-313X.2010.04166.x.
ClpR3 (ATP-dependent Clp protease proteolytic subunit-related protein 3, chloroplastic) is a serine-type endopeptidase located in chloroplast stoma. Alternative names: ClpR3, nClpP8.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica napus, Capsella rubella , Populus trichocarpa, Vitis viniferaSpecies of your interest not listed? Contact us
Immunogen:
BSA-conjugated peptide derived from ClpR3 of Arabidopsis thaliana, TAIR: AT1G09130 , UniProt:Q8L770
For western blot detection image refer to the article below
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
36 | 28,5 kDa
Not reactive in:
Zea mays
Selected references:
Sj gren et al. (2004). Inactivation of the clpC1 gene encoding a chloroplast Hsp100 molecular chaperone causes growth retardation, leaf chlorosis, lower photosynthetic activity, and a specific reduction in photosystem content. Plant Physiol. 2004 Dec;136(4):4114-26. Epub 2004 Nov 24.
ATG7 (Autophagy-related protein 7) is an E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 and ATG8 for its conjugation with phosphatidylethanolamine. Acts in the senescence process, degradation of damaged peroxisomes and non-selective degradation of chlorophylls and photosynthetic proteins during stress-induced leaf yellowing.Alternative names: Ubiquitin-like modifier-activating enzyme atg7, ATG12-activating enzyme E1 atg7, AtAPG7, Protein PEROXISOME UNUSUAL POSITIONING 4.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C (short tem, months) or at -80°C(long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica rapa, Capsella rubella , Citrus clementina, Noccaea caerulescens Species of your interest not listed? Contact us
Immunogen:
Recombinant ATG7 of Arabidopsis thaliana, UniProt: Q94CD5-1, TAIR: At5g45900, overexpressed in E.coli, collected in inclusion bodies, which were solubilized before immunization
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Doelling et al. (2002). The APG8/12-activating enzyme APG7 is required for proper nutrient recycling and senescence in Arabidopsis thaliana. J Biol Chem. 2002 Sep 6;277(36):33105-14.
Special application note:
Protein name was changed from APG7 to ATG7 after Doelling paper was published.
LUT5 (beta-carotene hydroxylase) is a heme-containing cytochrome P450 involved in the biosynthesis of xanthophylls. Enzyme is specific for beta-ring hydroxylation of alpha- and beta-carotene and displays a low activity toward the epsilon-rings of alpha-carotene. Alternative names: Cytochrome P450 97A3, Protein LUTEIN DEFICIENT 5, chloroplastic.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
His-tagged, recombinant LUT5 of Arabidopsis thaliana, overexpressed in E.coli, UniProt: Q93VK5,TAIR: AT1G31800
FBA (Fructose-bisphosphate aldolase, chloroplastic) is an enzyme involved in step 4 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Standard extraction protocol for leaf tissue can be applied. The antibody is directed on the unique peptide present in a chloroplast form of aldolase; it does not react with a cytosolic form in the Lolium-Festuca species.
Application Details:
1 :4000-1 : 8000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
42 | 38 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Fukayama et al. (2018). Expression level of Rubisco activase negatively correlates with Rubisco content in transgenic rice. Photosynth Res. 2018 May 30. doi: 10.1007/s11120-018-0525-9.Perlikowski et al. (2016). Water deficit affects primary metabolism differently in two Lolium multiflorum/Festuca arundinacea introgression forms with a distinct capacity for photosynthesis and membrane regeneration. Frontiers in Plant Science 7:1063. doi: 10.3389/fpls.2016.01063
Special application note:
This product can be sold containing ProClin if requested.
PPH1/TAP38 (Protein phosphatase 1) is a choroplast protein phosphatase TAP38/PPH1, required for efficient dephosphorylation of the LHCII anthena and state transition from state 2 to state 1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Cajanus cajan, Cephalotus follicularis, Cicer arietinum, Cucumis melo, Glycine soja, Gossypium hirsutum, Ilex paraguariensis, Mesembryanthemum crystallinum, Nelumbo nucifera, Nicotiana tabacum, Noccaea caerulescens, Populus trichocarpa, Ricinus communis, Theobroma caca, Vigna radiata var. radiata Species of your interest not listed? Contact us
ATG3 (Autophagy-related protein 3) is a E2 conjugating enzyme responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8. This step is required for the membrane association of ATG8. Alternative names: Autophagy-related E2-like conjugation enzyme ATG3, AtAPG3, Protein autophagy 3.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C (short tem, months) or at -80°C(long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica campestris, Eutrema salsugineum, Helianthus annuus, Nicotiana tabacum, Noccaea caerulescens, Populus trichocarpa, Salvia splendens, Sisymbrium irio, Solanum tuberosum Species of your interest not listed? Contact us
Immunogen:
Full length recombinant ATG3 of Arabidopsis thaliana protein sequence UniProt: Q0WWQ1-1, TAIR: At5g61500, overexpressed in E.coli
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Phillips et al. (2008). The ATG12-conjugating enzyme ATG10 Is essential for autophagic vesicle formation in Arabidopsis thaliana. Genetics. 2008 Mar;178(3):1339-53. doi: 10.1534/genetics.107.086199.
XLG2 protein is involved in G protein-coupled receptor signaling pathway and is involved in defense response, hypersensitive response and response to bacterium.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
Recombinant XLG2 of Arabidopsis thaliana UniProt: C6KIE6, TAIR: AT4G34390
ATG1a (Serine/threonine-protein kinase ATG1a) is a serine/threonine protein kinase involved in autophagy in a nutritional condition-dependent manner. The ATG1-ATG13 protein kinase complex regulates downstream events required for autophagosome enclosure and/or vacuolar delivery. Becomes a target of autophagy under nutrient starvation and connects autophagy to plant nutritional status. Alternative names: Autophagy-related protein 1a, AtAPG1a.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C (short tem, months) or at -80°C(long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Noccaea caerulescensSpecies of your interest not listed? Contact us
Immunogen:
Full length, N-terminally HIS-tagged ATG1a of Arabidopsis thaliana protein sequence UniProt: Q94C95, TAIR: At3g61960, overexpressed in E.coli and injected as a gel piece
Antibody may also recognise ATG1b and ATG1c; does not recognise ATG1t, Suttangkakul et al. (2011).
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l, of sterile water
Molecular Weight:
69,6 | 70 kDa
Not reactive in:
Oryza sativa
Selected references:
Suttangkakul et al. (2011). The ATG1/ATG13 protein kinase complex is both a regulator and a target of autophagic recycling in Arabidopsis. Plant Cell. 2011 Oct;23(10):3761-79. doi: 10.1105/tpc.111.090993.
Phosphoglucomutase is an enzyme which participates in both the breakdown and synthesis of glucose.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Zea mays
Expected Species:
Arabidopsis thaliana, Glycine max, Triticum aestivum Species of your interest not listed? Contact us
Immunogen:
Recombinant phosphoglucomutase of Zea mays, UniProt: P93804
Immunoprecipitation was performed by using Dynabeads Protein A: briefly 100 l suspension was washed with 200 l TTBS (Tris Buffered saline, 50 mM Tris-HCl pH 7.6 and 165 mM NaCl with 0.1% Tween 80) using the magnetic stands for concentrating the magnetic beads. After wash the beads were preincubated with 20 l primary antibodies in 180 l TTBS at room temperature for 30 minutes (minimum 15 minues). A first wash was followed afterwards with 200 l TTBS and hence a real incubation with 200 l plant extract (supernatant 20,000 x g for 3 min.), 200 l of TTBS and further 50 l YeastBuster reagent (Novagen) containing a mixture of detergents to break and solubilize the mitochondria membrane. This incubation at room temperature was allowed to be under mild shaking to allow the beads to be in suspension. Hence supernatant was aspirated away by the use of the magnetic stand and two further washesing steps with 200 l TTBS were performed prior mixing with 100 l SDS-Sample buffer.Antibody is recognizing recombinant PGM1, overexpressed in E.coli.
EIN3 (Ethylene insensitive 3) acts as a positive regulator in the ethylene response pathway and is required for ethylene responsiveness in adult plant tissues. Activated by phosphorylation by MPK3 and MPK6 (PubMed:18273012). Down-regulated by KIN10 that controls its protein stability under a phosphorylation-dependent manner. Alternative names: Protein ethylene insensitive 3.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C (short tem, months) or at -80°C(long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Populus trichocarpa
Immunogen:
Recombinant EIN3 of Arabidopsis thaliana , UniProt: O24606, TAIR: At3g20770
Western blot condtions for anti-EIN3 antibodies are described in Smalle et al. 2002.Recommended membrane for blocking: nitrocellulose, blocking reagent: 10 % non-fat milk, antibody incubation buffer: PBS-T.
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l, of sterile water
Molecular Weight:
71,4 | 78 kDa
Not reactive in:
Medicago truncatula
Selected references:
Park et al. (2023) Ethylene-triggered subcellular trafficking of CTR1 enhances the response to ethylene gas. Nat Commun. 2023;14(1):365. Published 2023 Jan 23. doi:10.1038/s41467-023-35975-6Gagne et al. (2004). Arabidopsis EIN3-binding F-box 1 and 2 form ubiquitin-protein ligases that repress ethylene action and promote growth by directing EIN3 degradation. Proc Natl Acad Sci U S A. 2004 Apr 27;101(17):6803-8.
PR-5 (Pathogenesis-related protein 5) is involved in plant pathogen defence.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana UniProt: P28493,TAIR:AT1G75040The peptide is not found in other thautamin-like proteins.
Propeptide 25,3 kD, processing aa 1-23, mature peptide 22,8 kD
UniProt number:
P28493
TAIR number:
AT1G75040
Research area:
Arabidopsis thaliana antibodies
Code:
Stress20
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