AKIN beta-1 (AKINB1) is a member of SnRK1/SNF1/AMPK family in plants. It is a regulatory subunit of the putative trimeric SNF1-related protein kinase (SnRK) comples which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide derived from Arabidopsis thaliana AKIN beta-1 Q84VQ1, At5g21170
Belda-Palaz n et al. (2020) A dual function of SnRK2 kinases in the regulation of SnRK1 and plant growth. Nat Plants. 2020 Nov;6(11):1345-1353. doi: 10.1038/s41477-020-00778-w. Epub 2020 Oct 19. PMID: 33077877.Crozet et al. (2016). SUMOylation represses SnRK1 signaling in Arabidopsis. Plant J. 2016 Jan;85(1):120-133. doi: 10.1111/tpj.13096.Emanuelle et al. (2015). SnRK1 from Arabidopsis thaliana is an atypical AMPK. Plant J. 2015 Mar 3. doi: 10.1111/tpj.12813.
AKIN beta-2 (AKINB2) is a member of SnRK1/SNF1/AMPK family in plants. It is a regulatory subunit of the putative trimeric SNF1-related protein kinase (SnRK) comples which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide derived from Arabidopsis thaliana AKIN beta-2 Q9SCY5
KC1 is a regulatory K+ channel subunit that assembles with different inward-rectifying K+ channels to affect their activities. The protein is expressed in extremely low amounts, e.g. a few houndred per cell. Alternative names: AKT4, AtKC1, potassium channel TKC
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide derived from Arabidopsis thaliana KC1 protein P92960, At4g32650
In the work of Honsbein et al, 125I has been used for detection of KC1 since this was the only way to get enough signal after 2-phase partitioning, ECL+ has been used with the protein after expression in Sf9 insect cells (1: 1000 primary antibody dilution) and in yeast with no problem (single band detected), but these are relatively high expression systems, In native plant material ion channels are expressed in ridiculously small quantities (a few hundred proteins per cell)
Application Details:
1 : 50 with 125I (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 100 l of sterile water
Molecular Weight:
75,5 | 75 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Honsbein et al. (2009). A tripartite SNARE-K+ channel complex mediates in channel-dependent K+ nutrition in Arabidopsis. The Plant Cell 21:2859-2877.
Special application note:
For detection images please, refer to the publication belowAntibody detects native and recombinant KC1
The Glycine decarboxylase complex (GDC) is abundant in mitochondria matrix of C3 leaves and functions in photorespiratory carbon recovery. GDC enzyme can account for up to 50% of matrix protein, and is responsible for the most prominent metabolic activity in the mitochondria of illuminated leaves, photorespiration. GDC is a multienzyme complex composed of four component enzymes, the P-, H-, T-, and L-proteins and is responsible for the conversion of glycine produced in the peroxisome to serine in the mitochondria during photorespiratory cycle. The H-protein plays a key role as a mobile substrate that commutes between the other subunits, allowing its lipoic acid “arm” to visit the active sites of the other three components.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
This antibody can be used on total cell extract of Arabidopsis thaliana.
Application Details:
1 : 5 000 (TP), (WB)
Purity:
Total IgG. Protein G purified in PBS pH 7.4.
Reconstitution:
For reconstitution add 200 l of sterile water
Molecular Weight:
16 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Sch fer et al. (2021) Assessment of Mitochondrial Protein Topology and Membrane Insertion. Methods Mol Biol. 2022;2363:165-181. doi: 10.1007/978-1-0716-1653-6_13. PMID: 34545493.Przybyla-Toscano et al. (2021) Protein lipoylation in mitochondria requires Fe-S cluster assembly factors NFU4 and NFU5. Plant Physiol. 2021 Oct 28:kiab501. doi: 10.1093/plphys/kiab501. Epub ahead of print. PMID: 34718778.Guralnick et al. (2020). The Development of Crassulacean Acid Metabolism (CAM) Photosynthesis in Cotyledons of the C4 Species, Portulaca grandiflora (Portulacaceae). Plants (Basel). 2020 Jan 2;9(1). pii: E55. doi: 10.3390/plants9010055. (tissue printing)R thor et al. (2019). Arabidopsis seedlings display a remarkable resilience under severe mineral starvation using their metabolic plasticity to remain self-sufficient for weeks. Plant J. 2019 Mar 22. doi: 10.1111/tpj.14325.Lynch et al. (2017). Multifaceted plant responses to circumvent Phe hyperaccumulation by downregulation of flux through the shikimate pathway and by vacuolar Phe sequestration. Plant J. 2017 Dec;92(5):939-950. doi: 10.1111/tpj.13730. (Petunia hybrida cv. Mitchell)
Special application note:
Cellular [compartment marker] of mitochondrial matrix
CBP20 (Nuclear cap-binding protein subunit 2)is a component of the cap-binding complex (CBC), involved in various processes such as pre-mRNA splicing and RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs). Alternative names: 20 kDa nuclear cap-binding protein, NCBP 20 kDa subunit
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Glycne max, Hordeum vulgare, Lotus corniculatus, Nicotiana tabacum, Oryza sativa, Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Zea mays Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide, derived with Arabidopsis thaliana CBP20 protein Q9xFD1, At5g44200
Thioredoxin Reductase (TR, TrxR) is the only known enzyme (EC 1.8.1.9) which is reducing thoredoxin (Trx) Involved in chloroplast protection against oxidative damage. Activity of thoredoxin is essential for growth and survival of the cell. There seem to be one isoform of NtrC protein in Arabidopsis thaliana, which includes an N-terminal reductase domain and a C-terminal domain related to thioredoxin proteins. Arabidopsis thaliana and Chlmydomonas reinhardtii NtrC proteins are ca. 568 amino acids long, but include ca. 80 amino acid signal peptides, for mature protein size of ca. 488 amino acids.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
KLH-conjugated peptide derived from N-terminal domain of NtrC of Chlamydomonas reinhardtii A8HNQ7The peptide is perfectly conserved in Arabidopsis thaliana NTRC, UniProt: , TAIR: AT2G41680
55.3 kDa (Chlamydomonas reinhardtii), 57.9 kDa (Arabidopsis thaliana), further processed into the matured form
Special application note:
Peptide target chosen from the N-terminal domain, nearly fully conserved within 3-4 NtrA-related proteins from Arabidopsis thalina and with NtrC related proteins from Chlamydomonas reinhardtii and Synechococcus sp. 7942 and other cyanobacteria
DHAR1 ( Dehydroascorbate Reductase 1) the protein is induced by jasmonic acid and oxidative chemical stresses and is a key component of the ascorbate recycling system. Involved in redox homeostasis under biotic and abiotic inducers. Localized in mitochondria. Synonymes: glutathione-dependent dehydroascorbate reductase 1, chloride intracellular channel homolog 1, CLIC homolog 1, glutathione-dependent dehydroascorbate reductase 1, AtDHAR1, GSH-dependent dehydroascorbate reductase 1, mtDHAR, AT1G19570.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Nicotiana tabacum, Populus trichocarpa, Ricinus communis, Solanum tuberosum, Triticum aestivum, Zinnia elegans Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from known DHAR1 sequence of Arabidopsis thaliana Q9FWR4, At1g19570
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Szymańska et al. (2019). SNF1-Related Protein Kinases SnRK2.4 and SnRK2.10 Modulate ROS Homeostasis in Plant Response to Salt Stress. Int J Mol Sci. 2019 Jan 2;20(1). pii: E143. doi: 10.3390/ijms20010143.Witzel et al. (2017). Temporal impact of the vascular wilt pathogen Verticillium dahliae on tomato root proteome. J Proteomics. 2017 Oct 3;169:215-224. doi: 10.1016/j.jprot.2017.04.008.Wang et al. (2014). Proteomic analysis of salt-responsive proteins in the leaves of mangrove Kandelia candel during short-term stress. PLoS One. 2014 Jan 8;9(1):e83141. doi: 10.1371/journal.pone.0083141. eCollection 2014.
AKT1 is a highly selective inward-rectifying potassium channel located in cell membrane, that mediates potassium uptake by plant roots in response to low potassium conditions.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide derived from Arabidopsis thaliana AKT1 Q38998 , At2g26650
In the work of Honsbein et al, 125I has been used for detection of KC1 since this was the only way to get enough signal after 2-phase partitioning, ECL+ has been used with the protein after expression in Sf9 insect cells (1: 1000 primary antibody dilution) and in yeast with no problem (single band detected), but these are relatively high expression systems, In native plant material ion channels are expressed in ridiculously small quantities (a few hundred proteins per cell)
Application Details:
1 : 50 with 125I (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 100 l of sterile water
Molecular Weight:
96,9 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Safiarian et al. (2015). Lost in traffic? The K+ channel of lily pollen, LilKT1, is detected at the endomembranes inside yeast cells, tobacco leaves and lily pollen. Front. Plant Sci. | doi: 10.3389/fpls.2015.00047.Honsbein et al. (2009). A tripartite SNARE-K+ channel complex mediates in channel-dependent K+ nutrition in Arabidopsis. The Plant Cell 21:2859-2877.
Special application note:
For detection images please, refer to the publication belowAntibody detects native and recombinant AKT1
Photosystem I (PSI) of chloroplasts is a multisubunit membrane-protein complex that catalyzes the electron transfer from the reduced plastocyanin (or cytochrome c6) in the thylakoid lumen to the oxidized ferredoxin (or flavodoxin) in the chloroplast stroma. PsaN is necessary for docking plastocyanin to the PSI complex. PSI-N is the only subunit located entirely on the lumenal side of PSI.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophikized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Hansson et al. (2007). Knock-out of the chloroplast encoded PSI-J Subunit of Photosystem I in Nicotiana tabacum: PSI-J is required for efficient electron transfer and stable accumulation of photosystem I. FEBS J. 274: 1734-1746.
VPS35 (Vacuolar Protein Sorting-associated protein 35) plays a role in vesicular protein sorting. Component of the membrane-associated retromer complex which is essential in endosome-to-Golgi retrograde transport. Also involved in the efficient sorting of seed storage proteins globulin 12S and albumin 2S. The VPS29-VPS26-VPS35 subcomplex may be involved in recycling of specific cargos from endosome to the plasma membrane. Arabidopsis thaliana has three VPS35 homologs designated VPS35a, VPS35b and VPS35c, and among them,VPS35b plays most important role in these processes. Localised to pre-vacuolar compartments (PVC).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 2 mg/ml.
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Molecular Weight:
89 | 98 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Yamazaki et al. (2008). Arabidopsis VPS35, a retromer component, is required for vacuolar protein sorting and involved in plant growth and leaf senescence. Plant Cell Physiol. 2008 Feb;49(2):142-56. doi: 10.1093/pcp/pcn006. (Immunofluorescence, Immunoprecipitation, Western blot)
VSP29 (Vacuolar protein sorting-associated protein 29) plays a role in vesicular protein sorting. Component of the membrane-associated retromer complex which is essential in endosome-to-Golgi retrograde transport. Required for the auxin-carrier protein PIN2 sorting to the lytic vacuolar pathway and the PIN1 recycling to the plasma membrane. Also involved in the efficient sorting of seed storage proteins globulin 12S and albumin 2S. The VPS29-VPS26-VPS35 subcomplex may be involved in recycling of specific cargos from endosome to the plasma membrane. Cellular localisation: endosome and Golgi apparatus.Alternative names: MAIGO1, Vesicle protein sorting 29
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 2 mg/ml.
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Molecular Weight:
21 | 25 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Yamazaki et al. (2008). Arabidopsis VPS35, a retromer component, is required for vacuolar protein sorting and involved in plant growth and leaf senescence. Plant Cell Physiol. 2008 Feb;49(2):142-56. doi: 10.1093/pcp/pcn006. (Immunoprecipitation, Western blot, Arabidopsis thaliana)Shimada et al. (2006). et al. (2006). AtVPS29, a putative component of a retromer complex, is required for the efficient sorting of seed storage proteins. Plant Cell Physiol. 2006 Sep;47(9):1187-94. doi: 10.1093/pcp/pcj103. (Western blot, Arabidopsis thaliana)
Plant vacuole V-ATPase is responsible for energization of transport of ions and metabolites, and acts as well 'house-keeping' and as a stress response enzyme. V-ATPase is a multi-subunit enzyme composed of a membrane sector and a cytosolic catalytic sector. It is related to the FoF1 ATP synthase. Alternative protein names: Vacuolar proton pump subunit E, Protein EMBRYO DEFECTIVE 2448
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
KLH-conjugated synthetic peptide chosen from subunit E of plant V-ATPase including Arabidopsis thaliana At4g11150. Peptide is conserved in vacuolar H+-ATPase subunit E, isoform 1 to 3 (VHA-E1).
V-ATPase is very sensitive for the redox of the SDS buffer. We recommend using at least 50-100 mM DTT freshly prepared before handling the sample.2 hours incubation with primary antibody is recommended over over night incubation which can contribute to increased background.
Application Details:
1: 600 (IF), 1 : 1000-1 : 3000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 100 l of sterile water
Molecular Weight:
26 | 31 kDa (Arabidopsis thaliana)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Thioredoxin Reductase (TR, TrxR) is the only known enzyme (EC 1.8.1.9) which is reducing thoredoxin (Trx). Activity of thoredoxin is essential for growth and survival of the cell. There seem to be one isoform of NtrC protein in Arabidopsis which includes an N-terminal reductase domain and a C-terminal domain related to thioredoxin proteins. Arabidopsis thaliana and Chlmydomonas reinhardtii NtrC proteins are ca. 568 amino acids long, but include ca. 80 amino acid signal peptides, for mature protein size of ca. 488 amino acids.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Expected Species:
Chlamydia sp., Marchantia polymorpha, Nannochloropsis gaditana, Ostreococcus luminarius, Physcomitrium patens, Synechococcus sp.Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from N-terminal domain of NtrC of Chlamydomonas reinhardtii A8HNQ7
Antibody will react with long NtrC of Chlamydomonas reinhardtii proteins and shorter NtrA-type rpoteins, which would be distinguished by migration size.
Application Details:
1 : 500 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 100 l of sterile water
Molecular Weight:
55,3 | 49 kDa (following processing)
Not reactive in:
Arabidopsis thaliana
Special application note:
Peptide target chosen from the N-terminal domain, nearly fully conserved within 3-4 NtrA-related proteins from Arabidopsis thalina and with NtrC related proteins from Chlamydomonas reinhardtii and Synechococcus sp. 7942 and other cyanobacteria
2S seed storage protein 3, one of major seed storage proteins is synthesized on the endoplasmic reticulum as precursor and then transported to storage vacuoles, where it is processed by an asparaginyl endopeptidase to produce two mature polypeptides referred to as large and small subunits which are linked by disulfide bonds. Subcellular localisation: vacuole.Alternative names: 2S albumin storage protein, NWMU2-2S albumin 3
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 2 mg/ml.
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
Conjugated peptide, derived from N-propeptide of Arabidopsis thaliana 2S3P, UniProt: P15459, TAIR: At4g27160
Applications:
ELISA (ELISA), Immunolocalization (IL) using electron microscopy, Western blot (WB)
Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Molecular Weight:
18,7 | 17-20 kDa (2S albumin precursors)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Shirakawa et al. (2014). CONTINUOUS VASCULAR RING (COV1) is a trans-Golgi network-localized membrane protein required for Golgi morphology and vacuolar protein sorting. Plant Cell Physiol. 2014 Apr;55(4):764-72. doi: 10.1093/pcp/pct195. (Western blot, Arabidopsis thaliana) Li et al. (2006). MAIGO2 is involved in exit of seed storage proteins from the endoplasmic reticulum in Arabidopsis thaliana. Plant Cell. 2006 Dec;18(12):3535-47. doi: 10.1105/tpc.106.046151.( Immunolocalisation by electron microscopy, Western blot, Arabidopsis thaliana)
VSP (Vegetative storage protein 1) may function as somatic storage protein during early seedling development. Expression of VSP1 is enhanced by wounding and protein is localized to vacuole.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
Full length, purified recombinant His6-tagged VSP1 of Arabidopsis thaliana UniProt: O49195, TAIR: At5g24780
Reactivity of this antibody to VSP2 has not been determined, Sequence conservation of VSP1 and VSP2 is 86 % therefore, it is most likely that this antibody will also recognize VSP2,
Application Details:
1: 1000 - 1: 2000 (WB)
Purity:
Total IgG, purified on Protein A in PBS, 50 % glycerol, filter sterilized.
Molecular Weight:
28 | 27 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Matsushima et al. (2002). An endoplasmic reticulum-derived structure that is induced under stress conditions in Arabidopsis.Plant Physiol. 2002 Dec;130(4):1807-14. doi: 10.1104/pp.009464.
2S seed storage protein 3, one of major seed storage proteins is synthesized on the endoplasmic reticulum as precursor and then transported to storage vacuoles, where it is processed by an asparaginyl endopeptidase to produce two mature polypeptides referred to as large and small subunits which are linked by disulfide bonds. Subcellular localisation: vacuole.Alternative names: 2S albumin storage protein, NWMU2-2S albumin 3
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 2 mg/ml.
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Capsella rubella Species of your interest not listed? Contact us
Immunogen:
Conjugated peptide, derived of Arabidopsis thaliana 2S3 large subunit, UniProt: P15459, TAIR: At4g27160
Applications:
ELISA (ELISA), Immunolocalization (IL) using electron microscopy, Western blot (WB)
Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Molecular Weight:
18, 7 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Takagi et al. (2013). MAIGO5 functions in protein export from Golgi-associated endoplasmic reticulum exit sites in Arabidopsis. Plant Cell . 2013 Nov;25(11):4658-75. doi: 10.1105/tpc.113.118158. (Immunolocalisation by electron microscopy, Western blot, Arabidopsis thaliana)
One of the several classes of mitochondrial proteases is membrane bound, ATPdependent FtsH protease. Their function is very important for the control of protein quality and quantity by degradation of unassembled subunits. Other names: AtFtsH3, cell division protease ftsH homolog 3, mitochondrial, AtFtsH10, cell division protease ftsH homolog 10, mitochondrial
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide dereived from sequences of Arabidopsis thaliana FtsH3 and FtsH10 with localization to mitochondria Q84WU8, At2g29080 and Q8VZI8, At1g07510
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Kolodziejczak et al. (2018). m-AAA Complexes Are Not Crucial for the Survival of Arabidopsis Under Optimal Growth Conditions Despite Their Importance for Mitochondrial Translation. Plant Cell Physiol. 2018 May 1;59(5):1006-1016. doi: 10.1093/pcp/pcy041.Piechota et al. (2010). Identification and characetization of high-molecular-weight complexes fromed by m-AAA proteases and prohibitins in mitochondria of Arabidopsis thaliana. J Biol Chem. 2010 Apr 23;285(17):12512-21. doi: 10.1074/jbc.M109.063644.
Special application note:
Blue-native (2D BN/SDS-PAGE) methodology is described in Piechota et al. 2010
PBP1 (PYK10-binding protein 1) is inhibitor-type lectin that may regulate the correct polymerization of BGLU23/PYK10 upon tissue damage. Activates BGLU21, BGLU22 and BGLU23. PBP1 is localised to cytoplasm.Alternative names: Jacalin-related lectin 30, Jasmonic acid-induced protein.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 2 mg/ml.
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
Conjugated peptide, derived from C-terminus of Arabidopsis thaliana PBP1, UniProt: O04314, TAIR: At3g16420
Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Molecular Weight:
32 | 35 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Nagano et al. (2005). Activation of an ER-body-localized beta-glucosidase via a cytosolic binding partner in damaged tissues of Arabidopsis thaliana. Plant Cell Physiol. 2005 Jul;46(7):1140-8. doi: 10.1093/pcp/pci126. (Immunofluorescence, Western Blot, Arabidopsis thaliana)
Oleosins may have a structural role to stabilize the lipid body during desiccation of the seed by preventing coalescence of the oil. Probably interacts with both lipid and phospholipid moieties of lipid bodies. May also provide recognition signals for specific lipase anchorage in lipolysis during seedling growth. Oleosins also increase the viability of over-wintering oilseeds by preventing abnormal fusion of oil bodies during imbibition in the spring. Cellular localisation: surface of oil bodies.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 2 mg/ml.
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica oleracea, Raphanus sativusSpecies of your interest not listed? Contact us
Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Molecular Weight:
18,5 | 17 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Shimada et al. (2010). A rapid and non-destructive screenable marker, FAST, for identifying transformed seeds of Arabidopsis thaliana. (Immunolocalisation)Shimada et al. (2008). A novel role for oleosins in freezing tolerance of oilseeds in Arabidopsis thaliana. Plant J. 2008 Sep;55(5):798-809. doi: 10.1111/j.1365-313X.2008.03553.x. (Western blot)
Oleosins may have a structural role to stabilize the lipid body during desiccation of the seed by preventing coalescence of the oil. Probably interacts with both lipid and phospholipid moieties of lipid bodies. May also provide recognition signals for specific lipase anchorage in lipolysis during seedling growth. Oleosins also increase the viability of over-wintering oilseeds by preventing abnormal fusion of oil bodies during imbibition in the spring. Cellular localisation: surface of oil bodies.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 2 mg/ml.
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Camelina sativa, Capsella rubella, utrema salsugineum. Raphanus sativus Species of your interest not listed? Contact us
Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Molecular Weight:
21 | 19 kDa
Not reactive in:
Glycine max
Selected references:
Shimada et al. (2008). A novel role for oleosins in freezing tolerance of oilseeds in Arabidopsis thaliana. Plant J. 2008 Sep;55(5):798-809. doi: 10.1111/j.1365-313X.2008.03553.x.
Major 12S seed storage protein CRC (globulin) is synthesized on the endoplasmic reticulum as precursor and then transported to storage vacuoles, where it is processed at a conserved Asn-Gly peptide bond by an asparaginyl endopeptidase to produce two mature polypeptides referred to as alpha and beta subunits that are joined together by a disulfide bond. Phosphorylated in seeds on some Tyr residues in response to abscisic acid (ABA). 12S CRC protein is cleaved to alpha and beta chains. Cellular localisation: vacuole.Alternative protein names: Cruciferin 3, Cruciferin C Legumin-type globulin Cruciferin1, Legumin-type globulin storage protein CRU1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 2 mg/ml.
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
Native, 12S globulin alpaha subunit purified from Arabidopsis thaliana and excised from SDS-PAGE gel. UniProt: Q96318, TAIR: At4g28520
Applications:
ELISA (ELISA), Immunolocalization (IL) using electron microscopy, Immunohistochemistry (IHC), Western blot (WB)
Assay dependent (ELISA), 1: 50 (IL by electron microscopy), 1: 100 (IHC), 1: 3000 - 1: 10 000 (WB)
Purity:
Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Molecular Weight:
58 | 30 kDa (alpha subunit)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Shirakawa et al. (2014). CONTINUOUS VASCULAR RING (COV1) is a trans-Golgi network-localized membrane protein required for Golgi morphology and vacuolar protein sorting. Plant Cell Physiol. 2014 Apr;55(4):764-72.doi: 10.1093/pcp/pct195. (Immunohistochemistry, Western blot)Li et al. MAG2 and three MAG2-INTERACTING PROTEINs form an ER-localized complex to facilitate storage protein transport in Arabidopsis thaliana. Plant J. 2013 Dec;76(5):781-91.doi: 10.1111/tpj.12347. (Immunolocalisation by electron microscopy, Western blot)
One of the several classes of mitochondrial proteases is membrane bound, ATPdependent FtsH protease. Their function is very important for the control of protein quality and quantity by degradation of unassembled subunits. Synonyme: cell division protease ftsH homolog 4, mitochondrial
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Brassica oleracea var. botrytis
Expected Species:
Brassica sp. Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from sequence of Arabidopsis thaliana FtsH4 UniProt: O80983, TAIR: At2g26140
Opalińska et al. (2017). Identification of Physiological Substrates and Binding Partners of the Plant Mitochondrial Protease FTSH4 by the Trapping Approach. Int J Mol Sci. 2017 Nov 18;18(11). pii: E2455. doi: 10.3390/ijms18112455.Dolzblasz et al. (2016). The mitochondrial protease AtFTSH4 safeguards Arabidopsis shoot apical meristem function. Sci Rep. 2016 Jun 20;6:28315. doi: 10.1038/srep28315.Rurek et al. (2015). Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, organellar translation and ultrastructure. Biochim Biophys Acta. 2015 Jan 21. pii: S0005-2728(15)00016-X. doi: 10.1016/j.bbabio.2015.01.005.Zhang et al. (2014). Perturbation of auxin homeostasis caused by mitochondrial FtSH4 gene-mediated peroxidase accumulation regulates Arabidopsis architechture. Mol Plant. 2014 Jan 30.Kwasniak et al. (2013). Silencing of the Nuclear RPS10 Gene Encoding Mitochondrial Ribosomal Protein Alters Translation in Arabidiopsis Mitochondria. Plant Cell, May 30.
BG1 (Beta-glucosidase 1) hydrolyzes abscisic acid glucose ester (ABA-GE) which represents the predominant form of conjugated ABA (biologically inactive). No activity with beta-D-glucopyranosyl zeatin. The hydrolysis of ABA-GE in the endoplasmic reticulum (ER) forms free ABA and contributes to increase its cellular levels under dehydration conditions. ABA-GE hydrolyzing activity is enhanced by dehydration stress-induced polymerization into higher molecular weight forms. The ABA produced by BGLU18 contributes to the initiation of intracellular signaling as well as the increase in the extracellular ABA level. Localised to ER lumen.Alternative names: AtBG1, Beta-glucosidase 18, Beta-glucosidase homolog 1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 2 mg/ml.
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
Purified recombinant BG1 of Arabidopsis thaliana, residues 27-528 with a His6-thioredoxin tagged, UniProt: Q9SE50, TAIR: At1g52400
Applications:
Immunolocalization (IL) using electron microscopy, Western blot (WB)
NAI2 TSA1-like protein, C-terminal is responsible for the ER body formation. Regulates the number and shape of the ER bodies and the accumulation of PYK10 in ER bodies, but is not involved in the expression of PYK10. Interacts directly or indirectly with MEB1 and MEB2. Expressed in roots. Detected in shoot apex. Localised to ER lumen.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 2 mg/ml.
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
Purified recombinant C-terminal part of NAI2 of Arabidopsis thaliana, residues 636-772 with a His tag, UniProt: Q9LSB4, TAIR: At3g15950
Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Molecular Weight:
85 | 120 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Yamada et al. (2008). NAI2 is an endoplasmic reticulum body component that enables ER body formation in Arabidopsis thaliana. Plant Cell . 2008 Sep;20(9):2529-40. doi: 10.1105/tpc.108.059345.
Special application note:
Signal peptide of 24 amino acids is removed from the mature protein
MEB2 (Membrane protein of ER body 2) may sequester excess cytosolic iron and manganese into endoplasmic reticulum to reduce metal ion toxicity. Not essential for the accumulation of ER body components, including PYK10.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 2 mg/ml.
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
Purified recombinant MEB2 of Arabidopsis thaliana, residues 1-325 with a His tag, UniProt: F4KFS7 , TAIR: At5g24290
Due to N-linked glycosylation at 9 asparagine residues 19-amino acids are cut of as signal peptide from N-terminus of MEB2,
Application Details:
1:1000 - 1: 2000 (IHC), 1: 10 000 (WB)
Purity:
Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Molecular Weight:
61 | 82 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Yamada et al. (2013). Identification of two novel endoplasmic reticulum body-specific integral membrane proteins. Plant Physiol . 2013 Jan;161(1):108-20. doi: 10.1104/pp.112.207654.
UniProt number:
F4KFS7
TAIR number:
At5g24290
Research area:
Arabidopsis thaliana antibodies
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