This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human COL3A1 (AA: 24-153) expressed in E. Coli.
This gene encodes the pro-alpha2 chain of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in this gene, however, tend to be less severe than mutations in the gene for the alpha1 chain of type I collagen (COL1A1) reflecting the different role of alpha2 chains in matrix integrity. Three transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human COL1A2 (AA: 23-79) expressed in E. Coli.
This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008]
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human COL1A1 (AA: 1219-1464) expressed in E. Coli.
This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008]
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human COL1A1 (AA: 1219-1464) expressed in E. Coli.
This gene encodes a protein with a markedly acidic C terminus; the basic N-terminus is highly homologous to the N-terminus of a related gene, CNN1. Members of the CNN gene family all contain similar tandemly repeated motifs. This encoded protein is associated with the cytoskeleton but is not involved in contraction.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CNN3 (AA: 26-130) expressed in E. Coli.
This gene encodes a protein with a markedly acidic C terminus; the basic N-terminus is highly homologous to the N-terminus of a related gene, CNN1. Members of the CNN gene family all contain similar tandemly repeated motifs. This encoded protein is associated with the cytoskeleton but is not involved in contraction.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CNN3 (AA: 26-130) expressed in E. Coli.
This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini. [provided by RefSeq, Aug 2017]
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human C-MYC (AA: (290-439)) expressed in E. Coli.
This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signaling, glycoprotein turnover, and roles in inflammation and immune response. The protein encoded by this gene is a negative regulator of granulocyte and monocyte function. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. This gene is closely linked to other CTL/CTLD superfamily members in the natural killer gene complex region on chromosome 12p13.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CLL1 (AA: extra 65-265) expressed in E. Coli.
This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signaling, glycoprotein turnover, and roles in inflammation and immune response. The protein encoded by this gene is a negative regulator of granulocyte and monocyte function. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. This gene is closely linked to other CTL/CTLD superfamily members in the natural killer gene complex region on chromosome 12p13.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CLL1 (AA: extra 65-265) expressed in E. Coli.
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. This gene encodes a component of tight junction strands, which is a member of the claudin family. The protein is an integral membrane protein and is one of the entry cofactors for hepatitis C virus. The gene methylation may be involved in esophageal tumorigenesis. This gene is adjacent to another family member CLDN9 on chromosome 16.[provided by RefSeq, Aug 2010]
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CLDN6 (AA: extra(29-81) and extra(138-160)) expressed in E. Coli.
The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CKM (AA: 1-381) expressed in E. Coli.
The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CKM (AA: 1-381) expressed in E. Coli.
The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. There is a related pseudogene located immediately adjacent to this locus.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD99 (AA: 1-185) expressed in E. Coli.
The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. There is a related pseudogene located immediately adjacent to this locus.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD99 (AA: 1-185) expressed in E. Coli.
This gene encodes a member of the EGF-TM7 subfamily of adhesion G protein-coupled receptors, which mediate cell-cell interactions. These proteins are cleaved by self-catalytic proteolysis into a large extracellular subunit and seven-span transmembrane subunit, which associate at the cell surface as a receptor complex. The encoded protein may play a role in cell adhesion as well as leukocyte recruitment, activation and migration, and contains multiple extracellular EGF-like repeats which mediate binding to chondroitin sulfate and the cell surface complement regulatory protein CD55. Expression of this gene may play a role in the progression of several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms with 3 to 5 EGF-like repeats have been observed for this gene. This gene is found in a cluster with other EGF-TM7 genes on the short arm of chromosome 19.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD97 (AA: extra 419-552) expressed in E. Coli.
The protein encoded by this gene belongs to the immunoglobulin superfamily. It is a type I membrane protein. The protein may play a role in the adhesive interactions of activated T and NK cells during the late phase of the immune response. It may also function in antigen presentation. Alternative splicing generates multiple transcript variants encoding distinct isoforms.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD96 (AA: extra 321-519) expressed in E. Coli.
The protein encoded by this gene belongs to the immunoglobulin superfamily. It is a type I membrane protein. The protein may play a role in the adhesive interactions of activated T and NK cells during the late phase of the immune response. It may also function in antigen presentation. Alternative splicing generates multiple transcript variants encoding distinct isoforms.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD96 (AA: extra 321-519) expressed in E. Coli.
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD8a (AA: 22235) expressed in E. Coli.
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD8a (AA: 22235) expressed in E. Coli.
This gene encodes the receptor for urokinase plasminogen activator and, given its role in localizing and promoting plasmin formation, likely influences many normal and pathological processes related to cell-surface plasminogen activation and localized degradation of the extracellular matrix. It binds both the proprotein and mature forms of urokinase plasminogen activator and permits the activation of the receptor-bound pro-enzyme by plasmin. The protein lacks transmembrane or cytoplasmic domains and may be anchored to the plasma membrane by a glycosyl-phosphatidylinositol (GPI) moiety following cleavage of the nascent polypeptide near its carboxy-terminus. However, a soluble protein is also produced in some cell types. Alternative splicing results in multiple transcript variants encoding different isoforms. The proprotein experiences several post-translational cleavage reactions that have not yet been fully defined.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD87 (AA: 23-305) expressed in E. Coli.
This gene encodes an immunoglobulin-like cell surface protein that is expressed predominantly on plasmacytoid dendritic cells (PDCs) and modulates the function of these cells in the immune response. Expression of this gene is downregulated by interleukin 3 (IL3). This gene is one of a cluster of highly related genes located at chromosomal region 19q13.4.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD85G (AA: extra 24-178) expressed in E. Coli.
This gene encodes an immunoglobulin-like cell surface protein that is expressed predominantly on plasmacytoid dendritic cells (PDCs) and modulates the function of these cells in the immune response. Expression of this gene is downregulated by interleukin 3 (IL3). This gene is one of a cluster of highly related genes located at chromosomal region 19q13.4.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD85G (AA: extra 24-178)) expressed in E. Coli.
The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. [provided by RefSeq, Aug 2011]
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD80 (AA: 35-288) expressed in E. Coli.
This gene encodes a transmembrane protein which is a member of the immunoglobulin superfamily. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD7 (AA: 26-180) expressed in E. Coli.
This gene encodes a transmembrane protein which is a member of the immunoglobulin superfamily. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD7 (AA: extra 26-180) expressed in E. Coli.
The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD61 (AA: extra 27-179) expressed in E. Coli.
The protein encoded by this gene is a member of the glucuronyltransferase gene family. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1 (human natural killer-1, also known as CD57 and LEU7). Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD57 (AA: 28-334) expressed in E. Coli.
The protein encoded by this gene is a member of the glucuronyltransferase gene family. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1 (human natural killer-1, also known as CD57 and LEU7). Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD57 (AA: 28-334) expressed in E. Coli.
The protein encoded by this gene is a member of the glucuronyltransferase gene family. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1 (human natural killer-1, also known as CD57 and LEU7). Alternate transcriptional splice variants have been characterized.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD57 (AA: 35-191) expressed in E. Coli.
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It contributes to the transduction of CD2-generated signals in T cells and natural killer cells and has been suggested to play a role in growth regulation. Familial deficiency of this gene has been linked to an immunodeficiency associated with recurrent infectious diseases caused by bacteria, fungi and viruses. Alternative splicing results in multiple transcript variants.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD53 (AA: extra mix) expressed in E. Coli.
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It contributes to the transduction of CD2-generated signals in T cells and natural killer cells and has been suggested to play a role in growth regulation. Familial deficiency of this gene has been linked to an immunodeficiency associated with recurrent infectious diseases caused by bacteria, fungi and viruses. Alternative splicing results in multiple transcript variants.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD53 (AA: extra mix) expressed in E. Coli.
This gene encodes a member of the scavenger receptor cysteine-rich (SRCR) superfamily. Members of this family are secreted or membrane-anchored proteins mainly found in cells associated with the immune system. This protein is a type-I transmembrane glycoprotein found on the surface of thymocytes, T lymphocytes and a subset of B lymphocytes. The encoded protein contains three SRCR domains and may act as a receptor to regulate T-cell proliferation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2016]
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD5 (AA: 403-495) expressed in E. Coli.
This gene encodes a member of the scavenger receptor cysteine-rich (SRCR) superfamily. Members of this family are secreted or membrane-anchored proteins mainly found in cells associated with the immune system. This protein is a type-I transmembrane glycoprotein found on the surface of thymocytes, T lymphocytes and a subset of B lymphocytes. The encoded protein contains three SRCR domains and may act as a receptor to regulate T-cell proliferation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2016]
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD5 (AA: 403-495) expressed in E. Coli.
This gene encodes a member of the scavenger receptor cysteine-rich (SRCR) superfamily. Members of this family are secreted or membrane-anchored proteins mainly found in cells associated with the immune system. This protein is a type-I transmembrane glycoprotein found on the surface of thymocytes, T lymphocytes and a subset of B lymphocytes. The encoded protein contains three SRCR domains and may act as a receptor to regulate T-cell proliferation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2016]
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD5 (AA: extra 25372) expressed in E. Coli.
The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 5 subunit. This subunit associates with the beta 1 subunit to form a fibronectin receptor. This integrin may promote tumor invasion, and higher expression of this gene may be correlated with shorter survival time in lung cancer patients. Note that the integrin alpha 5 and integrin alpha V subunits are encoded by distinct genes.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°Chttp://archive.promab.com/john/index.php for long term storage
Immunogen:
Purified recombinant fragment of human CD49E (AA: extra 111-253) expressed in E. Coli.
The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 5 subunit. This subunit associates with the beta 1 subunit to form a fibronectin receptor. This integrin may promote tumor invasion, and higher expression of this gene may be correlated with shorter survival time in lung cancer patients. Note that the integrin alpha 5 and integrin alpha V subunits are encoded by distinct genes.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD49E (AA: extra 111-253) expressed in E. Coli.
The gene encodes a member of the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 4 subunit. This subunit associates with a beta 1 or beta 7 subunit to form an integrin that may play a role in cell motility and migration. This integrin is a therapeutic target for the treatment of multiple sclerosis, Crohn's disease and inflammatory bowel disease. Alternative splicing results in multiple transcript variants.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD49D (AA: extra 833-977) expressed in E. Coli.
The gene encodes a member of the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 4 subunit. This subunit associates with a beta 1 or beta 7 subunit to form an integrin that may play a role in cell motility and migration. This integrin is a therapeutic target for the treatment of multiple sclerosis, Crohn's disease and inflammatory bowel disease. Alternative splicing results in multiple transcript variants.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD49D (AA: extra 833-977) expressed in E. Coli.
The protein encoded by this gene is a type I membrane protein and is a regulatory part of the complement system. The encoded protein has cofactor activity for inactivation of complement components C3b and C4b by serum factor I, which protects the host cell from damage by complement. In addition, the encoded protein can act as a receptor for the Edmonston strain of measles virus, human herpesvirus-6, and type IV pili of pathogenic Neisseria. Finally, the protein encoded by this gene may be involved in the fusion of the spermatozoa with the oocyte during fertilization. Mutations at this locus have been associated with susceptibility to hemolytic uremic syndrome. Alternatively spliced transcript variants encoding different isoforms have been described.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD46 (AA: extra 35-179) expressed in E. Coli.
The protein encoded by this gene is a type I membrane protein and is a regulatory part of the complement system. The encoded protein has cofactor activity for inactivation of complement components C3b and C4b by serum factor I, which protects the host cell from damage by complement. In addition, the encoded protein can act as a receptor for the Edmonston strain of measles virus, human herpesvirus-6, and type IV pili of pathogenic Neisseria. Finally, the protein encoded by this gene may be involved in the fusion of the spermatozoa with the oocyte during fertilization. Mutations at this locus have been associated with susceptibility to hemolytic uremic syndrome. Alternatively spliced transcript variants encoding different isoforms have been described.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD46 (AA: extra 35-179) expressed in E. Coli.
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD45R (AA: extra 378-577) expressed in E. Coli.
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD45R (AA: extra 378-577) expressed in E. Coli.
This gene encodes a highly sialylated glycoprotein that functions in antigen-specific activation of T cells, and is found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It contains a mucin-like extracellular domain, a transmembrane region and a carboxy-terminal intracellular region. The extracellular domain has a high proportion of serine and threonine residues, allowing extensive O-glycosylation, and has one potential N-glycosylation site, while the carboxy-terminal region has potential phosphorylation sites that may mediate transduction of activation signals. Different glycoforms of this protein have been described. In stimulated immune cells, proteolytic cleavage of the extracellular domain occurs in some cell types, releasing a soluble extracellular fragment. Defects in expression of this gene are associated with Wiskott-Aldrich syndrome.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD43 (AA: extra 20-253) expressed in E. Coli.
This gene encodes a highly sialylated glycoprotein that functions in antigen-specific activation of T cells, and is found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It contains a mucin-like extracellular domain, a transmembrane region and a carboxy-terminal intracellular region. The extracellular domain has a high proportion of serine and threonine residues, allowing extensive O-glycosylation, and has one potential N-glycosylation site, while the carboxy-terminal region has potential phosphorylation sites that may mediate transduction of activation signals. Different glycoforms of this protein have been described. In stimulated immune cells, proteolytic cleavage of the extracellular domain occurs in some cell types, releasing a soluble extracellular fragment. Defects in expression of this gene are associated with Wiskott-Aldrich syndrome.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD43(AA: extra(20-169)) expressed in E. Coli.
This gene encodes a small membrane glycoprotein found on the surface of human platelets. It forms a 1-to-1 noncovalent complex with glycoprotein Ib, a platelet surface membrane glycoprotein complex that functions as a receptor for von Willebrand factor. The complete receptor complex includes noncovalent association of the alpha and beta subunits with the protein encoded by this gene and platelet glycoprotein V. Defects in this gene are a cause of Bernard-Soulier syndrome, also known as giant platelet disease. These patients have unusually large platelets and have a clinical bleeding tendency.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD42A (AA: extra 17-147) expressed in E. Coli.
This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2014]
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD40 (AA: extra(21-193)) expressed in E. Coli.
This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2014]
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD40 (AA: extra(21-193)) expressed in E. Coli.
The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined. [provided by RefSeq, Feb 2009]
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD3D (AA: 127-171) expressed in E. Coli.
The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined. [provided by RefSeq, Feb 2009]
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD3D (AA: 127-171) expressed in E. Coli.
The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD3D (AA: 127-171) expressed in E. Coli.
This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. The encoded glycoprotein is a small type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif. It functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD369 (AA: extra 66-247) expressed in E. Coli.
This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. The encoded glycoprotein is a small type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif. It functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD369 (AA: extra 66-247) expressed in E. Coli.
This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. [provided by RefSeq, Jul 2008]
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD368 (AA: extra 39-215) expressed in E. Coli.
This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. [provided by RefSeq, Jul 2008]
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD368 (AA: extra 39-215) expressed in E. Coli.
This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type 2 transmembrane protein may play a role in inflammatory and immune response. Multiple transcript variants encoding distinct isoforms have been identified for this gene. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD367 (AA: extra 70-237) expressed in E. Coli.
The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. Alternative splicing results in multiple transcript variants.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD363 expressed in E. Coli.
The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. Alternative splicing results in multiple transcript variants.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD363 expressed in E. Coli.
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human EPCAM (AA: extra(116-265)) expressed in E. Coli.
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human EPCAM (AA: extra(116-265)) expressed in E. Coli.
This gene encodes a methyltransferase that methylates lysine residues of histone H3. Methylation of H3 at lysine 9 by this protein results in recruitment of additional epigenetic regulators and repression of transcription. This gene was initially thought to be two different genes, NG36 and G9a, adjacent to each other in the HLA locus. Alternative splicing results in multiple transcript variants.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human EHMT2 (AA: 317-471) expressed in E. Coli.
This gene encodes a methyltransferase that methylates lysine residues of histone H3. Methylation of H3 at lysine 9 by this protein results in recruitment of additional epigenetic regulators and repression of transcription. This gene was initially thought to be two different genes, NG36 and G9a, adjacent to each other in the HLA locus. Alternative splicing results in multiple transcript variants.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human EHMT2 (AA: 317-471) expressed in E. Coli.
EVI2B is required for granulocyte differentiation and functionality of hematopoietic progenitor cells through the control of cell cycle progression and survival of hematopoietic progenitor cells.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD361 (AA: extra 22-202) expressed in E. Coli.
The protein encoded by this gene is a cytokine receptor for interleukin 21 (IL21). It belongs to the type I cytokine receptors, and has been shown to form a heterodimeric receptor complex with the common gamma-chain, a receptor subunit also shared by the receptors for interleukin 2, 4, 7, 9, and 15. This receptor transduces the growth promoting signal of IL21, and is important for the proliferation and differentiation of T cells, B cells, and natural killer (NK) cells. The ligand binding of this receptor leads to the activation of multiple downstream signaling molecules, including JAK1, JAK3, STAT1, and STAT3. Knockout studies of a similar gene in mouse suggest a role for this gene in regulating immunoglobulin production. Three alternatively spliced transcript variants have been described.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD360 (AA: extra 20-232) expressed in E. Coli.
The protein encoded by this gene is a cytokine receptor for interleukin 21 (IL21). It belongs to the type I cytokine receptors, and has been shown to form a heterodimeric receptor complex with the common gamma-chain, a receptor subunit also shared by the receptors for interleukin 2, 4, 7, 9, and 15. This receptor transduces the growth promoting signal of IL21, and is important for the proliferation and differentiation of T cells, B cells, and natural killer (NK) cells. The ligand binding of this receptor leads to the activation of multiple downstream signaling molecules, including JAK1, JAK3, STAT1, and STAT3. Knockout studies of a similar gene in mouse suggest a role for this gene in regulating immunoglobulin production. Three alternatively spliced transcript variants have been described.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD360 (AA: extra 20-232) expressed in E. Coli.
The CRTAM gene is upregulated in CD4 (see MIM 186940)-positive and CD8 (see CD8A; MIM 186910)-positive T cells and encodes a type I transmembrane protein with V and C1-like Ig domains (Yeh et al., 2008 [PubMed 18329370]).
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD355 (AA: extra 18-287) expressed in E. Coli.
This gene encodes a receptor belonging to the Ig superfamily that is expressed on myeloid cells. This protein amplifies neutrophil and monocyte-mediated inflammatory responses triggered by bacterial and fungal infections by stimulating release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD354 (AA: extra 21-205) expressed in E. Coli.
This gene encodes a receptor belonging to the Ig superfamily that is expressed on myeloid cells. This protein amplifies neutrophil and monocyte-mediated inflammatory responses triggered by bacterial and fungal infections by stimulating release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD354 (AA: extra 21-205) expressed in E. Coli.
This gene encodes a member of the CD2 family of cell surface proteins involved in lymphocyte activation. These proteins are characterized by Ig domains. This protein is expressed in lymphoid tissues, and studies of a similar protein in mouse suggest that it may function during B cell lineage commitment. The gene is found in a region of chromosome 1 containing many CD2 genes.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD353 (AA: extra 23-233) expressed in E. Coli.
The protein encoded by this gene is a type I transmembrane protein, belonging to the CD2 subfamily of the immunoglobulin superfamily. This encoded protein is expressed on Natural killer (NK), T, and B lymphocytes. It undergoes tyrosine phosphorylation and associates with the Src homology 2 domain-containing protein (SH2D1A) as well as with SH2 domain-containing phosphatases (SHPs). It functions as a coreceptor in the process of NK cell activation. It can also mediate inhibitory signals in NK cells from X-linked lymphoproliferative patients. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD352 (AA: extra 22-226) expressed in E. Coli.
The protein encoded by this gene is a type I transmembrane protein, belonging to the CD2 subfamily of the immunoglobulin superfamily. This encoded protein is expressed on Natural killer (NK), T, and B lymphocytes. It undergoes tyrosine phosphorylation and associates with the Src homology 2 domain-containing protein (SH2D1A) as well as with SH2 domain-containing phosphatases (SHPs). It functions as a coreceptor in the process of NK cell activation. It can also mediate inhibitory signals in NK cells from X-linked lymphoproliferative patients. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD352 (AA: extra 22-226) expressed in E. Coli.
The protein encoded by this gene is a type I transmembrane protein, belonging to the CD2 subfamily of the immunoglobulin superfamily. This encoded protein is expressed on Natural killer (NK), T, and B lymphocytes. It undergoes tyrosine phosphorylation and associates with the Src homology 2 domain-containing protein (SH2D1A) as well as with SH2 domain-containing phosphatases (SHPs). It functions as a coreceptor in the process of NK cell activation. It can also mediate inhibitory signals in NK cells from X-linked lymphoproliferative patients. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD352 (AA: extra 22-226) expressed in E. Coli.
FCAMR as a receptor for the Fc fragment of IgA and IgM. Binds IgA and IgM with high affinity and mediates their endocytosis. May function in the immune response to microbes mediated by IgA and IgM.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD351 (AA: extra 221-450) expressed in E. Coli.
This gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This protein may play a role as a positive regulator of the Wingless type MMTV integration site signaling pathway. A transcript variant retaining intronic sequence and encoding a shorter isoform has been described, however, its expression is not supported by other experimental evidence.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD344 (AA: extra 37-222) expressed in E. Coli.
The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD34 (AA: extra 32-290) expressed in E. Coli.
The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD339 (AA: extra 47-212) expressed in E. Coli.
The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD339 (AA: extra 47-212) expressed in E. Coli.
The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD339 (AA: extra 47-212) expressed in E. Coli.
NCR2 is a Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD336 (AA: extra 22-192) expressed in E. Coli.
This gene encodes a member of the immunoglobulin receptor superfamily and is one of several Fc receptor-like glycoproteins clustered on the long arm of chromosome 1. The encoded protein contains three extracellular C2-like immunoglobulin domains, a transmembrane domain and a cytoplasmic domain with two immunoreceptor-tyrosine activation motifs. This protein may play a role in the regulation of cancer cell growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD307A (AA: extra 17-202) expressed in E. Coli.
The protein encoded by this gene is a member of the immunoglobulin superfamily. It was identified by its similarity to leukocyte-associated immunoglobulin-like receptor 1, a membrane-bound receptor that modulates innate immune response. The protein encoded by this locus is a soluble receptor that may play roles in both inhibition of collagen-induced platelet aggregation and vessel formation during placental implantation. This gene maps to a region of 19q13.4, termed the leukocyte receptor cluster, which contains 29 genes in the immunoglobulin superfamily. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2013]
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD306 (AA: 22-152) expressed in E. Coli.
This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type 2 transmembrane protein may play a role in dendritic cell function. Two transcript variants encoding distinct isoforms have been identified for this gene.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD303 (AA: extra 45-213) expressed in E. Coli.
This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type 2 transmembrane protein may function as a cell surface antigen. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD301 (AA: extra 158-316) expressed in E. Coli.
The CMRF35 antigen, which was identified by reactivity with a monoclonal antibody, is present on monocytes, neutrophils, and some T and B lymphocytes (Jackson et al., 1992 [PubMed 1349532]).
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD300C (AA: 21-183) expressed in E. Coli.
This gene encodes a member of the CD300 glycoprotein family of cell surface proteins found on leukocytes involved in immune response signaling pathways. This gene is located on chromosome 17 in a cluster with all but one of the other family members. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD300A (AA: extra 18-180) expressed in E. Coli.
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD30 (AA: extra 19-379) expressed in E. Coli.
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD30 (AA: extra 19-379) expressed in E. Coli.
This gene encodes a transmembrane receptor and is often referred to as L-SIGN because of its expression in the endothelial cells of the lymph nodes and liver. The encoded protein is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses, with a large impact on public health. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain and C-type lectin carbohydrate recognition domain. The extracellular region consisting of the C-type lectin and neck domains has a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells. The neck region is important for homo-oligomerization which allows the receptor to bind multivalent ligands with high avidity. Variations in the number of 23 amino acid repeats in the neck domain of this protein are common and have a significant impact on ligand binding ability. This gene is closely related in terms of both sequence and function to a neighboring gene (GeneID 30835; often referred to as DC-SIGN or CD209). DC-SIGN and L-SIGN differ in their ligand-binding properties and distribution. Alternative splicing results in multiple variants.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD299 (AA: extra 237-399) expressed in E. Coli.
The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is preferentially expressed in immune cell rich tissues, such as spleen, lymph node, bone marrow and peripheral blood leukocytes. Studies in mice and human indicate that this receptor mediates cellular response to unmethylated CpG dinucleotides in bacterial DNA to mount an innate immune response.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD289 (AA: extra 26-193) expressed in E. Coli.
The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection, and induces the activation of NF-kappaB and the production of type I interferons. It may thus play a role in host defense against viruses. Use of alternative polyadenylation sites to generate different length transcripts has been noted for this gene. [provided by RefSeq, Jul 2008]
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD283 (AA: 24-217) expressed in E. Coli.
The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. This protein is a cell-surface protein that can form heterodimers with other TLR family members to recognize conserved molecules derived from microorganisms known as pathogen-associated molecular patterns (PAMPs). Activation of TLRs by PAMPs leads to an up-regulation of signaling pathways to modulate the host's inflammatory response. This gene is also thought to promote apoptosis in response to bacterial lipoproteins. This gene has been implicated in the pathogenesis of several autoimmune diseases. Alternative splicing results in multiple transcript variants.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD282 (AA: extra 21-197) expressed in E. Coli.
Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function (By similarity).
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD275 (AA: extra 19-256) expressed in E. Coli.
This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD274 (AA: 24-153) expressed in E. Coli.
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD27 (AA: extra 20-144) expressed in E. Coli.
The protein encoded by this gene is a lymphocyte-specific member of the tumor necrosis factor (TNF) receptor superfamily. It interacts with calcium-modulator and cyclophilin ligand (CAML). The protein induces activation of the transcription factors NFAT, AP1, and NF-kappa-B and plays a crucial role in humoral immunity by interacting with a TNF ligand. This gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD267 (AA: extra(1-165)) expressed in E. Coli.
The protein encoded by this gene is a lymphocyte-specific member of the tumor necrosis factor (TNF) receptor superfamily. It interacts with calcium-modulator and cyclophilin ligand (CAML). The protein induces activation of the transcription factors NFAT, AP1, and NF-kappa-B and plays a crucial role in humoral immunity by interacting with a TNF ligand. This gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD267 (AA: extra(1-165)) expressed in E. Coli.
The protein encoded by this gene is a lymphocyte-specific member of the tumor necrosis factor (TNF) receptor superfamily. It interacts with calcium-modulator and cyclophilin ligand (CAML). The protein induces activation of the transcription factors NFAT, AP1, and NF-kappa-B and plays a crucial role in humoral immunity by interacting with a TNF ligand. This gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD267 (AA: extra(1-165)) expressed in E. Coli.
The protein encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF17/BCMA, a member of the TNF receptor family. This protein and its receptor are both found to be important for B cell development. In vitro experiments suggested that this protein may be able to induce apoptosis through its interaction with other TNF receptor family proteins such as TNFRSF6/FAS and TNFRSF14/HVEM. Alternative splicing results in multiple transcript variants. Some transcripts that skip the last exon of the upstream gene (TNFSF12) and continue into the second exon of this gene have been identified; such read-through transcripts are contained in GeneID 407977, TNFSF12-TNFSF13.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD256 (AA: 105-250) expressed in E. Coli.
CD248 (CD248 Molecule) is a Protein Coding gene. Gene Ontology (GO) annotations related to this gene include calcium ion binding and extracellular matrix binding. An important paralog of this gene is CD93.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD248 (AA: extra 18-180) expressed in E. Coli.
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. Mutations in this gene are associated with colchicine resistance and Inflammatory bowel disease 13. Alternative splicing and the use of alternative promoters results in multiple transcript variants.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD243 (AA: 1149-1280) expressed in E. Coli.
This gene encodes Lutheran blood group glycoprotein, a member of the immunoglobulin superfamily and a receptor for the extracellular matrix protein, laminin. The protein contains five extracellular immunoglobulin domains, a single transmembrane domain, and a short C-terminal cytoplasmic tail. This protein may play a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. Polymorphisms in this gene define some of the antigens in the Lutheran system and also the Auberger system. Inactivating variants of this gene result in the recessive Lutheran null phenotype, Lu(a-b-), of the Lutheran blood group. Two transcript variants encoding different isoforms have been found for this gene.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD239 (AA: extra 32-197) expressed in E. Coli.
This gene encodes Lutheran blood group glycoprotein, a member of the immunoglobulin superfamily and a receptor for the extracellular matrix protein, laminin. The protein contains five extracellular immunoglobulin domains, a single transmembrane domain, and a short C-terminal cytoplasmic tail. This protein may play a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. Polymorphisms in this gene define some of the antigens in the Lutheran system and also the Auberger system. Inactivating variants of this gene result in the recessive Lutheran null phenotype, Lu(a-b-), of the Lutheran blood group. Two transcript variants encoding different isoforms have been found for this gene.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD239 (AA: extra 32-197) expressed in E. Coli.
This gene encodes a member of the plexin family. Plexins are transmembrane receptors for semaphorins, a large family of proteins that regulate axon guidance, cell motility and migration, and the immune response. The encoded protein and its ligand regulate melanocyte adhesion, and viral semaphorins may modulate the immune response by binding to this receptor. The encoded protein may be a tumor suppressor protein for melanoma. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jan 2011]
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD232 (AA: extra 35-234) expressed in E. Coli.
This gene encodes a member of the plexin family. Plexins are transmembrane receptors for semaphorins, a large family of proteins that regulate axon guidance, cell motility and migration, and the immune response. The encoded protein and its ligand regulate melanocyte adhesion, and viral semaphorins may modulate the immune response by binding to this receptor. The encoded protein may be a tumor suppressor protein for melanoma. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jan 2011]
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD232 (AA: extra 35-234) expressed in E. Coli.
The protein encoded by this gene is a membrane glycosylphosphatidylinositol-anchored glycoprotein that tends to aggregate into rod-like structures. The encoded protein contains a highly unstable region of five tandem octapeptide repeats. This gene is found on chromosome 20, approximately 20 kbp upstream of a gene which encodes a biochemically and structurally similar protein to the one encoded by this gene. Mutations in the repeat region as well as elsewhere in this gene have been associated with Creutzfeldt-Jakob disease, fatal familial insomnia, Gerstmann-Straussler disease, Huntington disease-like 1, and kuru. An overlapping open reading frame has been found for this gene that encodes a smaller, structurally unrelated protein, AltPrp. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD230 (AA: 23-230) expressed in E. Coli.
The enzyme encoded by this gene is a type I gamma-glutamyltransferase that catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. The enzyme is composed of a heavy chain and a light chain, which are derived from a single precursor protein. It is expressed in tissues involved in absorption and secretion and may contribute to the etiology of diabetes and other metabolic disorders. Multiple alternatively spliced variants have been identified. There are a number of related genes present on chromosomes 20 and 22, and putative pseudogenes for this gene on chromosomes 2, 13, and 22. [provided by RefSeq, Jan 2014]
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD224 (AA: extra 381-470) expressed in E. Coli.
This gene encodes a receptor for both insulin-like growth factor 2 and mannose 6-phosphate. The binding sites for each ligand are located on different segments of the protein. This receptor has various functions, including in the intracellular trafficking of lysosomal enzymes, the activation of transforming growth factor beta, and the degradation of insulin-like growth factor 2. Mutation or loss of heterozygosity of this gene has been association with risk of hepatocellular carcinoma. The orthologous mouse gene is imprinted and shows exclusive expression from the maternal allele; however, imprinting of the human gene may be polymorphic, as only a minority of individuals showed biased expression from the maternal allele (PMID:8267611). [provided by RefSeq, Nov 2015]
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD222 (AA: 160-311) expressed in E. Coli.
This gene encodes a receptor for both insulin-like growth factor 2 and mannose 6-phosphate. The binding sites for each ligand are located on different segments of the protein. This receptor has various functions, including in the intracellular trafficking of lysosomal enzymes, the activation of transforming growth factor beta, and the degradation of insulin-like growth factor 2. Mutation or loss of heterozygosity of this gene has been association with risk of hepatocellular carcinoma. The orthologous mouse gene is imprinted and shows exclusive expression from the maternal allele; however, imprinting of the human gene may be polymorphic, as only a minority of individuals showed biased expression from the maternal allele (PMID:8267611). [provided by RefSeq, Nov 2015]
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD222 (AA: 160-311) expressed in E. Coli.
The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This receptor specifically binds interleukin 18 (IL18), and is essential for IL18 mediated signal transduction. IFN-alpha and IL12 are reported to induce the expression of this receptor in NK and T cells. This gene along with four other members of the interleukin 1 receptor family, including IL1R2, IL1R1, ILRL2 (IL-1Rrp2), and IL1RL1 (T1/ST2), form a gene cluster on chromosome 2q. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD218A (AA: extra 22-185) expressed in E. Coli.
Interleukin 17A (IL17A) is a proinflammatory cytokine secreted by activated T-lymphocytes. It is a potent inducer of the maturation of CD34-positive hematopoietic precursors into neutrophils. The transmembrane protein encoded by this gene (interleukin 17A receptor; IL17RA) is a ubiquitous type I membrane glycoprotein that binds with low affinity to interleukin 17A. Interleukin 17A and its receptor play a pathogenic role in many inflammatory and autoimmune diseases such as rheumatoid arthritis. Like other cytokine receptors, this receptor likely has a multimeric structure. Alternative splicing results in multiple transcript variants encoding different isoforms.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD217 (AA: extra 33-320) expressed in E. Coli.
This gene encodes a cytokine receptor that specifically binds interleukin 15 (IL15) with high affinity. The receptors of IL15 and IL2 share two subunits, IL2R beta and IL2R gamma. This forms the basis of many overlapping biological activities of IL15 and IL2. The protein encoded by this gene is structurally related to IL2R alpha, an additional IL2-specific alpha subunit necessary for high affinity IL2 binding. Unlike IL2RA, IL15RA is capable of binding IL15 with high affinity independent of other subunits, which suggests distinct roles between IL15 and IL2. This receptor is reported to enhance cell proliferation and expression of apoptosis inhibitor BCL2L1/BCL2-XL and BCL2. Multiple alternatively spliced transcript variants of this gene have been reported.[provided by RefSeq, Apr 2010]
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD215 (AA: extra 31-205) expressed in E. Coli.
The protein encoded by this gene is a subunit of the interleukin 13 receptor. This subunit forms a receptor complex with IL4 receptor alpha, a subunit shared by IL13 and IL4 receptors. This subunit serves as a primary IL13-binding subunit of the IL13 receptor, and may also be a component of IL4 receptors. This protein has been shown to bind tyrosine kinase TYK2, and thus may mediate the signaling processes that lead to the activation of JAK1, STAT3 and STAT6 induced by IL13 and IL4.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD213A1 (AA: extra 22-343) expressed in E. Coli.
This gene encodes a membrane protein, which functions as a receptor for Epstein-Barr virus (EBV) binding on B and T lymphocytes. Genetic variations in this gene are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9). Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD21 (AA: extra 740-964) expressed in E. Coli.
Dendritic cells (DCs) are the most potent antigen-presenting cells. Immature DCs efficiently capture antigens and differentiate into interdigitating dendritic cells (IDCs) in lymphoid tissues that induce primary T-cell responses (summary by de Saint-Vis et al., 1998 [PubMed 9768752]).
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD208 (AA: 218-381) expressed in E. Coli.
The protein encoded by this gene is expressed only in Langerhans cells which are immature dendritic cells of the epidermis and mucosa. It is localized in the Birbeck granules, organelles present in the cytoplasm of Langerhans cells and consisting of superimposed and zippered membranes. It is a C-type lectin with mannose binding specificity, and it has been proposed that mannose binding by this protein leads to internalization of antigen into Birbeck granules and providing access to a nonclassical antigen-processing pathway. Mutations in this gene result in Birbeck granules deficiency or loss of sugar binding activity. [provided by RefSeq, Aug 2010]
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD207 (AA: 65-328) expressed in E. Coli.
This gene encodes the class A macrophage scavenger receptors, which include three different types (1, 2, 3) generated by alternative splicing of this gene. These receptors or isoforms are macrophage-specific trimeric integral membrane glycoproteins and have been implicated in many macrophage-associated physiological and pathological processes including atherosclerosis, Alzheimer's disease, and host defense. The isoforms type 1 and type 2 are functional receptors and are able to mediate the endocytosis of modified low density lipoproteins (LDLs). The isoform type 3 does not internalize modified LDL (acetyl-LDL) despite having the domain shown to mediate this function in the types 1 and 2 isoforms. It has an altered intracellular processing and is trapped within the endoplasmic reticulum, making it unable to perform endocytosis. The isoform type 3 can inhibit the function of isoforms type 1 and type 2 when co-expressed, indicating a dominant negative effect and suggesting a mechanism for regulation of scavenger receptor activity in macrophages.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD204 (AA: extra 275-451) expressed in E. Coli.
This gene encodes a receptor that belongs to the protein tyrosine kinase Tie2 family. The encoded protein possesses a unique extracellular region that contains two immunoglobulin-like domains, three epidermal growth factor (EGF)-like domains and three fibronectin type III repeats. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Mutations in this gene are associated with inherited venous malformations of the skin and mucous membranes. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD202B (AA: extra 571-748) expressed in E. Coli.
This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. The gene is preferentially expressed by immature dendritic cells and memory T cells. The ligand of this receptor is macrophage inflammatory protein 3 alpha (MIP-3 alpha). This receptor has been shown to be important for B-lineage maturation and antigen-driven B-cell differentiation, and it may regulate the migration and recruitment of dentritic and T cells during inflammatory and immunological responses. Alternatively spliced transcript variants that encode the same protein have been described for this gene.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD196 expressed in E. Coli.
The protein encoded by this gene is a receptor for monocyte chemoattractant protein-1, a chemokine which specifically mediates monocyte chemotaxis. Monocyte chemoattractant protein-1 is involved in monocyte infiltration in inflammatory diseases such as rheumatoid arthritis as well as in the inflammatory response against tumors. The encoded protein mediates agonist-dependent calcium mobilization and inhibition of adenylyl cyclase. This protein can also be a coreceptor with CD4 for HIV-1 infection. This gene is located in the chemokine receptor gene cluster region of chromosome 3.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD192 expressed in E. Coli.
The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. This receptor also binds to chemokine (C-X-C motif) ligand 1 (CXCL1/MGSA), a protein with melanoma growth stimulating activity, and has been shown to be a major component required for serum-dependent melanoma cell growth. This receptor mediates neutrophil migration to sites of inflammation. The angiogenic effects of IL8 in intestinal microvascular endothelial cells are found to be mediated by this receptor. Knockout studies in mice suggested that this receptor controls the positioning of oligodendrocyte precursors in developing spinal cord by arresting their migration. This gene, IL8RA, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36. Alternatively spliced variants, encoding the same protein, have been identified.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD182 expressed in E. Coli.
The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. This receptor also binds to chemokine (C-X-C motif) ligand 1 (CXCL1/MGSA), a protein with melanoma growth stimulating activity, and has been shown to be a major component required for serum-dependent melanoma cell growth. This receptor mediates neutrophil migration to sites of inflammation. The angiogenic effects of IL8 in intestinal microvascular endothelial cells are found to be mediated by this receptor. Knockout studies in mice suggested that this receptor controls the positioning of oligodendrocyte precursors in developing spinal cord by arresting their migration. This gene, IL8RA, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36. Alternatively spliced variants, encoding the same protein, have been identified.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD182 expressed in E. Coli.
The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. Knockout studies in mice suggested that this protein inhibits embryonic oligodendrocyte precursor migration in developing spinal cord. This gene, IL8RB, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36. [provided by RefSeq, Jul 2008]
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD181 expressed in E. Coli.
This gene encodes an integrin beta chain, which combines with multiple different alpha chains to form different integrin heterodimers. Integrins are integral cell-surface proteins that participate in cell adhesion as well as cell-surface mediated signalling. The encoded protein plays an important role in immune response and defects in this gene cause leukocyte adhesion deficiency. Alternative splicing results in multiple transcript variants.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD18 (AA: extra 559-700) expressed in E. Coli.
This gene encodes an integrin beta chain, which combines with multiple different alpha chains to form different integrin heterodimers. Integrins are integral cell-surface proteins that participate in cell adhesion as well as cell-surface mediated signalling. The encoded protein plays an important role in immune response and defects in this gene cause leukocyte adhesion deficiency. Alternative splicing results in multiple transcript variants.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD18 (AA: extra 559-700) expressed in E. Coli.
The protein encoded by this gene belongs to the immunoglobulin superfamily and is expressed selectively at the early stages of B cell development, namely, in proB and early preB cells. This gene encodes the iota polypeptide chain that is associated with the Ig-mu chain to form a molecular complex which is expressed on the surface of pre-B cells. The complex is thought to regulate Ig gene rearrangements in the early steps of B-cell differentiation. Alternative splicing results in multiple transcript variants.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD179A (AA: extra 20-145) expressed in E. Coli.
This gene encodes a glycosyl-phosphatidylinositol (GPI)-linked cell surface glycoprotein that plays a role in neutrophil activation. The protein can bind platelet endothelial cell adhesion molecule-1 and function in neutrophil transmigration. Mutations in this gene are associated with myeloproliferative diseases. Over-expression of this gene has been found in patients with polycythemia rubra vera. Autoantibodies against the protein may result in pulmonary transfusion reactions, and it may be involved in Wegener's granulomatosis. A related pseudogene, which is adjacent to this gene on chromosome 19, has been identified.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD177 (AA: extra 22-161) expressed in E. Coli.
This gene encodes a glycosyl-phosphatidylinositol (GPI)-linked cell surface glycoprotein that plays a role in neutrophil activation. The protein can bind platelet endothelial cell adhesion molecule-1 and function in neutrophil transmigration. Mutations in this gene are associated with myeloproliferative diseases. Over-expression of this gene has been found in patients with polycythemia rubra vera. Autoantibodies against the protein may result in pulmonary transfusion reactions, and it may be involved in Wegener's granulomatosis. A related pseudogene, which is adjacent to this gene on chromosome 19, has been identified.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD177 (AA: extra 22-161) expressed in E. Coli.
The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Mutations in this gene are responsible for the majority of Lewis antigen-negative phenotypes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008]
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD174 (AA: 199-361) expressed in E. Coli.
The protein encoded by this gene is a member of the signal-regulatory protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. Alternatively spliced transcript variants encoding different isoforms have been described.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD172G (AA: extra 29-360) expressed in E. Coli.
The protein encoded by this gene is a member of the signal-regulatory protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. Alternatively spliced transcript variants encoding different isoforms have been described.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD172G (AA: extra 29-360) expressed in E. Coli.
The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of this gene results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD171 (AA: 20-197) expressed in E. Coli.
This gene encodes a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. These cell surface lectins are characterized by structural motifs in the immunoglobulin (Ig)-like domains and sialic acid recognition sites in the first Ig V set domain. The encoded protein is a member of the CD33-related subset of Siglecs and inhibits the activation of several cell types including monocytes, macrophages and neutrophils. Binding of group B Streptococcus (GBS) to the encoded protein plays a role in GBS immune evasion.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD170 (AA: extra 17-230) expressed in E. Coli.
This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a lectin-like adhesion molecule that binds glycoconjugate ligands on cell surfaces in a sialic acid-dependent manner. It is a type I transmembrane protein expressed only by a subpopulation of macrophages and is involved in mediating cell-cell interactions. Alternative splicing produces a transcript variant encoding an isoform that is soluble rather than membrane-bound; however, the full-length nature of this variant has not been determined.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD169 (AA: extra 20-197) expressed in E. Coli.
The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD163 (AA: extra 42-259) expressed in E. Coli.
The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD163 (AA: extra 42-259) expressed in E. Coli.
The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. Alternative splicing results in multiple transcript variants.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CHRM5 expressed in E. Coli.
The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The clinical implications of this receptor are unknown; however, stimulation of this receptor is known to increase cyclic AMP levels.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CHRM5 expressed in E. Coli.
The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 3 controls smooth muscle contraction and its stimulation causes secretion of glandular tissue. Alternative promoter use and alternative splicing results in multiple transcript variants that have different tissue specificities.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CHRM3 (AA: extra mix) expressed in E. Coli.
The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Two other peptides, catestatin and chromofungin, have antimicrobial activity and antifungal activity, respectively. Two transcript variants encoding different isoforms have been found for this gene.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CHGA (AA: 278-457) expressed in E. Coli.
The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Two other peptides, catestatin and chromofungin, have antimicrobial activity and antifungal activity, respectively. Two transcript variants encoding different isoforms have been found for this gene.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CHGA (AA: 278-457) expressed in E. Coli.
The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. Somatic mutations in this gene are associated with serous endometrial tumors. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014]
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CHD4 (AA: 1-194) expressed in E. Coli.
This gene encodes a cell surface glycoprotein that represents the founding member of the carcinoembryonic antigen (CEA) family of proteins. The encoded protein is used as a clinical biomarker for gastrointestinal cancers and may promote tumor development through its role as a cell adhesion molecule. Additionally, the encoded protein may regulate differentiation, apoptosis, and cell polarity. This gene is present in a CEA family gene cluster on chromosome 19. Alternative splicing results in multiple transcript variants.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CEACAM5 (AA: 35-165) expressed in E. Coli.
This gene is a member of the caudal-related homeobox transcription factor gene family. The encoded protein is a major regulator of intestine-specific genes involved in cell growth an differentiation. This protein also plays a role in early embryonic development of the intestinal tract. Aberrant expression of this gene is associated with intestinal inflammation and tumorigenesis. [provided by RefSeq, Jan 2012]
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CDX2 (AA: 1-180) expressed in E. Coli.
This gene is a member of the caudal-related homeobox transcription factor gene family. The encoded protein is a major regulator of intestine-specific genes involved in cell growth an differentiation. This protein also plays a role in early embryonic development of the intestinal tract. Aberrant expression of this gene is associated with intestinal inflammation and tumorigenesis. [provided by RefSeq, Jan 2012]
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CDX2 (AA: 1-180) expressed in E. Coli.
This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CDKN2A/P16 (AA: 1-156) expressed in E. Coli.
This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CDKN2A (AA: 1-156) expressed in E. Coli.
This gene is imprinted, with preferential expression of the maternal allele. The encoded protein is a tight-binding, strong inhibitor of several G1 cyclin/Cdk complexes and a negative regulator of cell proliferation. Mutations in this gene are implicated in sporadic cancers and Beckwith-Wiedemann syndorome, suggesting that this gene is a tumor suppressor candidate. Three transcript variants encoding two different isoforms have been found for this gene.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CDKN1C expressed in E. Coli.
This gene is imprinted, with preferential expression of the maternal allele. The encoded protein is a tight-binding, strong inhibitor of several G1 cyclin/Cdk complexes and a negative regulator of cell proliferation. Mutations in this gene are implicated in sporadic cancers and Beckwith-Wiedemann syndorome, suggesting that this gene is a tumor suppressor candidate. Three transcript variants encoding two different isoforms have been found for this gene.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CDKN1C expressed in E. Coli.
Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies among haplotypes, although several "framework" genes are found in all haplotypes (KIR3DL3, KIR3DP1, KIR3DL4, KIR3DL2). The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus, KIR proteins are thought to play an important role in regulation of the immune response. This gene is one of the "framework" loci that is present on all haplotypes. Alternate alleles of this gene are represented on multiple alternate reference loci (ALT_REF_LOCs). Alternative splicing results in multiple transcript variants, some of which may not be annotated on the primary reference assembly.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD158D (AA: extra 22-120) expressed in E. Coli.
Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies among haplotypes, although several "framework" genes are found in all haplotypes (KIR3DL3, KIR3DP1, KIR3DL4, KIR3DL2). The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus, KIR proteins are thought to play an important role in regulation of the immune response. This gene is one of the "framework" loci that is present on all haplotypes. Alternate alleles of this gene are represented on multiple alternate reference loci (ALT_REF_LOCs). Alternative splicing results in multiple transcript variants, some of which may not be annotated on the primary reference assembly.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD158D (AA: extra 22-120) expressed in E. Coli.
This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene may be involved in cell adhesion during neurodegeneration, and it is thought to be a target for allergic respiratory diseases, including asthma. Alternative splicing results in multiple transcript variants.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD156 (AA: extra 17-156) expressed in E. Coli.
This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene may be involved in cell adhesion during neurodegeneration, and it is thought to be a target for allergic respiratory diseases, including asthma. Alternative splicing results in multiple transcript variants.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD156 (AA: extra 17-156) expressed in E. Coli.
The protein encoded by this gene is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. The external domain mediates cell attachment to the extracellular matrix molecule vitronectin, while its intracellular domain interacts with the dynein light chain Tctex-1/DYNLT1. The gene is specific to the primate lineage, and serves as a cellular receptor for poliovirus in the first step of poliovirus replication. Multiple transcript variants encoding different isoforms have been found for this gene.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD155 (AA: 21-343) expressed in E. Coli.
The product of this gene transfers fucose to N-acetyllactosamine polysaccharides to generate fucosylated carbohydrate structures. It catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15). [provided by RefSeq, Jan 2009]
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD15 (AA: 1-147) expressed in E. Coli.
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes, including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region containing five fibronectin type III repeats, a single transmembrane region, and a single intracytoplasmic catalytic domain, and thus represents a receptor-type PTP. This protein is present in all hematopoietic lineages, and was shown to negatively regulate T cell receptor signaling possibly through interfering with the phosphorylation of Phospholipase C Gamma 1 and Linker for Activation of T Cells. This protein can also dephosphorylate the PDGF beta receptor, and may be involved in UV-induced signal transduction. Multiple transcript variants encoding different isoforms have been found for this gene.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD148 (AA: extra 36-210) expressed in E. Coli.
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes, including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region containing five fibronectin type III repeats, a single transmembrane region, and a single intracytoplasmic catalytic domain, and thus represents a receptor-type PTP. This protein is present in all hematopoietic lineages, and was shown to negatively regulate T cell receptor signaling possibly through interfering with the phosphorylation of Phospholipase C Gamma 1 and Linker for Activation of T Cells. This protein can also dephosphorylate the PDGF beta receptor, and may be involved in UV-induced signal transduction. Multiple transcript variants encoding different isoforms have been found for this gene.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD148 (AA: extra 36-210) expressed in E. Coli.
The protein encoded by this gene is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. The encoded protein is also a member of the immunoglobulin superfamily. Multiple transcript variants encoding different isoforms have been found for this gene.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD147 (AA: extra 138-323) expressed in E. Coli.
The protein encoded by this gene is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. The encoded protein is also a member of the immunoglobulin superfamily. Multiple transcript variants encoding different isoforms have been found for this gene.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD147 (AA: extra 138-323) expressed in E. Coli.
This gene encodes an enzyme involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This enzyme plays a key role in the renin-angiotensin system. Many studies have associated the presence or absence of a 287 bp Alu repeat element in this gene with the levels of circulating enzyme or cardiovascular pathophysiologies. Multiple alternatively spliced transcript variants encoding different isoforms have been identified, and two most abundant spliced variants encode the somatic form and the testicular form, respectively, that are equally active.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD143 (AA: extra 30-182) expressed in E. Coli.
The protein encoded by this intronless gene is an endothelial-specific type I membrane receptor that binds thrombin. This binding results in the activation of protein C, which degrades clotting factors Va and VIIIa and reduces the amount of thrombin generated. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD141 (AA: extra 297-505) expressed in E. Coli.
The protein encoded by this gene is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggest that this gene plays a critical role in regulating myocyte apoptosis. Alternatively spliced transcript variants have been described. A related pseudogene has been identified on chromosome 17. [provided by RefSeq, May 2014]
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD130 (AA: extra 73-231) expressed in E. Coli.
The protein encoded by this gene is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggest that this gene plays a critical role in regulating myocyte apoptosis. Alternatively spliced transcript variants have been described. A related pseudogene has been identified on chromosome 17. [provided by RefSeq, May 2014]
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD130 (AA: extra 73-231) expressed in E. Coli.
The protein encoded by this gene is a receptor for interleukin 7 (IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukins 2, 4, 7, 9, and 15. This protein has been shown to play a critical role in V(D)J recombination during lymphocyte development. Defects in this gene may be associated with severe combined immunodeficiency (SCID). Alternatively spliced transcript variants have been found.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD127 (AA: extra 21-239) expressed in E. Coli.
The protein encoded by this gene is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4. Several alternatively spliced transcript variants encoding four distinct isoforms have been reported.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD125 (AA: extra 21-196) expressed in E. Coli.
This gene encodes the alpha chain of the interleukin-4 receptor, a type I transmembrane protein that can bind interleukin 4 and interleukin 13 to regulate IgE production. The encoded protein also can bind interleukin 4 to promote differentiation of Th2 cells. A soluble form of the encoded protein can be produced by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. Allelic variations in this gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema. Polymorphisms in this gene are also associated with resistance to human immunodeficiency virus type-1 infection. Alternate splicing results in multiple transcript variants.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD124 (AA: 26-232) expressed in E. Coli.
The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. The function of IAPs in TNF-receptor signalling is unknown, however, c-IAP1 is thought to potentiate TNF-induced apoptosis by the ubiquitination and degradation of TNF-receptor-associated factor 2, which mediates anti-apoptotic signals. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD120B (AA: extra 23-257) expressed in E. Coli.
The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. The function of IAPs in TNF-receptor signalling is unknown, however, c-IAP1 is thought to potentiate TNF-induced apoptosis by the ubiquitination and degradation of TNF-receptor-associated factor 2, which mediates anti-apoptotic signals. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD120B (AA: extra 23-257) expressed in E. Coli.
This gene belongs to the beta-2 integrin family of membrane glycoproteins, which are are composed of non-covalently linked alpha and beta subunits to form a heterodimer. It encodes the alpha subunit of the cell surface heterodimers and is involved in the activation and adhesion functions of leukocytes. The gene is located about 11kb downstream of the integrin subunit alpha X gene, another member of the integrin family. It is expressed in the tissue and circulating myeloid leukocytes. Alternative splicing results in multiple transcript variants.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD11D (AA: extra 18-172) expressed in E. Coli.
ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. Two transcript variants encoding different isoforms have been found for this gene.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD11a (AA: extra 26-194) expressed in E. Coli.
This gene encodes a protein that belongs to the type I cytokine receptor family. This protein combines with a high-affinity converter subunit, gp130, to form a receptor complex that mediates the action of the leukemia inhibitory factor, a polyfunctional cytokine that is involved in cellular differentiation, proliferation and survival in the adult and the embryo. Mutations in this gene cause Schwartz-Jampel syndrome type 2, a disease belonging to the group of the bent-bone dysplasias. A translocation that involves the promoter of this gene, t(5;8)(p13;q12) with the pleiomorphic adenoma gene 1, is associated with salivary gland pleiomorphic adenoma, a common type of benign epithelial tumor of the salivary gland. Multiple splice variants encoding two different isoforms have been found for this gene.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD118 (AA: 45-188) expressed in E. Coli.
This gene encodes a protein that belongs to the type I cytokine receptor family. This protein combines with a high-affinity converter subunit, gp130, to form a receptor complex that mediates the action of the leukemia inhibitory factor, a polyfunctional cytokine that is involved in cellular differentiation, proliferation and survival in the adult and the embryo. Mutations in this gene cause Schwartz-Jampel syndrome type 2, a disease belonging to the group of the bent-bone dysplasias. A translocation that involves the promoter of this gene, t(5;8)(p13;q12) with the pleiomorphic adenoma gene 1, is associated with salivary gland pleiomorphic adenoma, a common type of benign epithelial tumor of the salivary gland. Multiple splice variants encoding two different isoforms have been found for this gene.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD118 (AA: 45-188) expressed in E. Coli.
This gene encodes a member of the nectin family of proteins, which function as adhesion molecules at adherens junctions. This family member interacts with other nectin-like proteins and with afadin, a filamentous actin-binding protein involved in the regulation of directional motility, cell proliferation and survival. This gene plays a role in ocular development involving the ciliary body. Mutations in this gene are believed to result in congenital ocular defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD113 (AA: extra 282-404) expressed in E. Coli.
This gene encodes a member of the semaphorin family of proteins. The encoded preproprotein is proteolytically processed to generate the mature glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein. The encoded protein is found on activated lymphocytes and erythrocytes and may be involved in immunomodulatory and neuronal processes. The encoded protein carries the John Milton Hagen (JMH) blood group antigens. Mutations in this gene may be associated with reduced bone mineral density (BMD). Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD108 (AA: 48-222) expressed in E. Coli.
CD101 (CD101 Molecule) is a Protein Coding gene. Diseases associated with CD101 include Langerhans Cell Histiocytosis and Histiocytosis. Among its related pathways are Innate Lymphoid Cell Differentiation Pathways and Innate Immune System. Gene Ontology (GO) annotations related to this gene include hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides. An important paralog of this gene is IGSF3.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD101 (AA: extra 22-168) expressed in E. Coli.
CD101 (CD101 Molecule) is a Protein Coding gene. Diseases associated with CD101 include Langerhans Cell Histiocytosis and Histiocytosis. Among its related pathways are Innate Lymphoid Cell Differentiation Pathways and Innate Immune System. Gene Ontology (GO) annotations related to this gene include hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides. An important paralog of this gene is IGSF3.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD101 (AA: extra 22-168) expressed in E. Coli.
The protein encoded by this gene is a type II transmembrane glycoprotein and a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). The encoded protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD10 (AA: extra 549-750) expressed in E. Coli.
The protein encoded by this gene is a type II transmembrane glycoprotein and a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). The encoded protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD10 (AA: 52-246) expressed in E. Coli.
The protein encoded by this gene is a type II transmembrane glycoprotein and a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). The encoded protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD10 (AA: extra 549-750) expressed in E. Coli.
The protein encoded by this gene is a type II transmembrane glycoprotein and a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). The encoded protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD10 (AA: extra 549-750) expressed in E. Coli.
This gene encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. This gene, which encodes a 100-kD type II transmembrane glycoprotein, exists in a single copy of greater than 45 kb. The 5' untranslated region of this gene is alternatively spliced, resulting in four separate mRNA transcripts. The coding region is not affected by alternative splicing.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD10 (AA: 321-496) expressed in E. Coli.
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. [provided by RefSeq, Apr 2016]
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CCNE1 (AA: 1-100) expressed in E. Coli.
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. [provided by RefSeq, Apr 2016]
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CCNE1 (AA: 1-100) expressed in E. Coli.
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of human cancers.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CCND1 (AA: 1-295) expressed in E. Coli.
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of human cancers.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CCND1 (AA: 1-295) expressed in E. Coli.
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CASP9 (AA: 331-416) expressed in E. Coli.
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family of enzymes. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic acid residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspases 7, 8 and 10, and is thought to function as a downstream enzyme in the caspase activation cascade. Alternative splicing of this gene results in multiple transcript variants that encode different isoforms.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CASP6 (AA: 194293) expressed in E. Coli.
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family of enzymes. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic acid residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspases 7, 8 and 10, and is thought to function as a downstream enzyme in the caspase activation cascade. Alternative splicing of this gene results in multiple transcript variants that encode different isoforms.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CASP6 (AA: 194293) expressed in E. Coli.
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CAPN2 (AA: 489-700) expressed in E. Coli.
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CAPN1 (AA: 501-714) expressed in E. Coli.
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CAPN1 (AA: 501-714) expressed in E. Coli.
Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CALR (AA: 18-417) expressed in E. Coli.
This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CALD1 (AA: 26-207) expressed in E. Coli.
This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CALD1 (AA: 26-207) expressed in E. Coli.
The protein encoded by this gene is a tyrosine kinase and cell surface receptor for fibroblast growth factors. The encoded protein is involved in the regulation of several pathways, including cell proliferation, cell differentiation, cell migration, lipid metabolism, bile acid biosynthesis, vitamin D metabolism, glucose uptake, and phosphate homeostasis. This protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment, and a cytoplasmic tyrosine kinase domain. The extracellular portion interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. [provided by RefSeq, Aug 2017]
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD334 (AA: extra 22-369) expressed in E. Coli.
The protein encoded by this gene is a tyrosine kinase and cell surface receptor for fibroblast growth factors. The encoded protein is involved in the regulation of several pathways, including cell proliferation, cell differentiation, cell migration, lipid metabolism, bile acid biosynthesis, vitamin D metabolism, glucose uptake, and phosphate homeostasis. This protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment, and a cytoplasmic tyrosine kinase domain. The extracellular portion interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. [provided by RefSeq, Aug 2017]
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD334 (AA: extra 22-369) expressed in E. Coli.
SIGLEC7 (Sialic Acid Binding Ig Like Lectin 7) is a Protein Coding gene. Diseases associated with SIGLEC7 include Congenital Disorder Of Glycosylation, Type Iic. Among its related pathways are Hematopoietic Stem Cells and Lineage-specific Markers and Innate Immune System. Gene Ontology (GO) annotations related to this gene include carbohydrate binding. An important paralog of this gene is SIGLEC12.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD328 (AA: extra(19-142)) expressed in E. Coli.
This gene encodes a member of the SIGLEC (sialic acid binding immunoglobulin-like lectin) family of proteins. The encoded transmembrane receptor binds sialyl-TN glycans and leptin. Placental expression of the encoded protein is upregulated in preeclampsia.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD327 (AA: extra 27-347) expressed in E. Coli.
This gene belongs to the immunoglobulin superfamily, and the junctional adhesion molecule (JAM) family. The protein encoded by this gene is a type I membrane protein that is localized at the tight junctions of both epithelial and endothelial cells. It acts as an adhesive ligand for interacting with a variety of immune cell types, and may play a role in lymphocyte homing to secondary lymphoid organs. Alternatively spliced transcript variants have been found for this gene.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD322 (AA: extra 29-238) expressed in E. Coli.
This gene belongs to the immunoglobulin superfamily, and the junctional adhesion molecule (JAM) family. The protein encoded by this gene is a type I membrane protein that is localized at the tight junctions of both epithelial and endothelial cells. It acts as an adhesive ligand for interacting with a variety of immune cell types, and may play a role in lymphocyte homing to secondary lymphoid organs. Alternatively spliced transcript variants have been found for this gene.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD322 (AA: extra 29-238) expressed in E. Coli.
Inhibits the binding of prostaglandin F2-alpha (PGF2-alpha) to its specific FP receptor, by decreasing the receptor number rather than the affinity constant. Functional coupling with the prostaglandin F2-alpha receptor seems to occur (By similarity).
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD315 (AA: extra 46-221) expressed in E. Coli.
Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. They can also regulate specific humoral and cell-mediated immunity. NK cells preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. The NKG2 gene family is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells. This gene encodes a member of the NKG2 family. The encoded transmembrane protein is characterized by a type II membrane orientation (has an extracellular C terminus) and the presence of a C-type lectin domain. It binds to a diverse family of ligands that include MHC class I chain-related A and B proteins and UL-16 binding proteins, where ligand-receptor interactions can result in the activation of NK and T cells. The surface expression of these ligands is important for the recognition of stressed cells by the immune system, and thus this protein and its ligands are therapeutic targets for the treatment of immune diseases and cancers. Read-through transcription exists between this gene and the upstream KLRC4 (killer cell lectin-like receptor subfamily C, member 4) family member in the same cluster.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD314 (AA: extra 73-216) expressed in E. Coli.
Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. They can also regulate specific humoral and cell-mediated immunity. NK cells preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. The NKG2 gene family is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells. This gene encodes a member of the NKG2 family. The encoded transmembrane protein is characterized by a type II membrane orientation (has an extracellular C terminus) and the presence of a C-type lectin domain. It binds to a diverse family of ligands that include MHC class I chain-related A and B proteins and UL-16 binding proteins, where ligand-receptor interactions can result in the activation of NK and T cells. The surface expression of these ligands is important for the recognition of stressed cells by the immune system, and thus this protein and its ligands are therapeutic targets for the treatment of immune diseases and cancers. Read-through transcription exists between this gene and the upstream KLRC4 (killer cell lectin-like receptor subfamily C, member 4) family member in the same cluster.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD314 (AA: extra 73-216) expressed in E. Coli.
This gene encodes a member of the immunoglobulin receptor superfamily and the Fc-receptor like family. This gene and several other Fc receptor-like gene members are clustered on the long arm of chromosome 1. The encoded protein is a single-pass type I membrane protein and contains 8 immunoglobulin-like C2-type domains. This gene is implicated in B cell development and lymphomagenesis. Alternatively spliced transcript variants encoding different isoforms have been identified.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD307E (AA: extra 16-158) expressed in E. Coli.
This gene encodes a member of the immunoglobulin receptor superfamily and is one of several Fc receptor-like glycoproteins clustered on the long arm of chromosome 1. The encoded protein contains immunoreceptor-tyrosine activation motifs and immunoreceptor-tyrosine inhibitory motifs in its cytoplasmic domain and may play a role in regulation of the immune system. Mutations in this gene have been associated with rheumatoid arthritis, autoimmune thyroid disease, and systemic lupus erythematosus. Alternative splicing results in multiple transcript variants.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD307C (AA: extra 18-153) expressed in E. Coli.
This gene encodes a member of the immunoglobulin receptor superfamily and is one of several Fc receptor-like glycoproteins clustered on the long arm of chromosome 1. The encoded protein contains immunoreceptor-tyrosine activation motifs and immunoreceptor-tyrosine inhibitory motifs in its cytoplasmic domain and may play a role in regulation of the immune system. Mutations in this gene have been associated with rheumatoid arthritis, autoimmune thyroid disease, and systemic lupus erythematosus. Alternative splicing results in multiple transcript variants.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD307C (AA: extra 18-153) expressed in E. Coli.
This gene encodes a member of the immunoglobulin receptor superfamily and is one of several Fc receptor-like glycoproteins clustered on the long arm of chromosome 1. The encoded protein has four extracellular C2-type immunoglobulin domains, a transmembrane domain and a cytoplasmic domain that contains one immunoreceptor-tyrosine activation motif and two immunoreceptor-tyrosine inhibitory motifs. This protein may be a prognostic marker for chronic lymphocytic leukemia. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD307B (AA: extra 20-253) expressed in E. Coli.
This gene encodes a member of the immunoglobulin receptor superfamily and is one of several Fc receptor-like glycoproteins clustered on the long arm of chromosome 1. The encoded protein contains three extracellular C2-like immunoglobulin domains, a transmembrane domain and a cytoplasmic domain with two immunoreceptor-tyrosine activation motifs. This protein may play a role in the regulation of cancer cell growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD307A (AA: extra 17-202) expressed in E. Coli.
This gene encodes the A-chain polypeptide of serum complement subcomponent C1q, which associates with C1r and C1s to yield the first component of the serum complement system. C1q deficiency is associated with lupus erythematosus and glomerulonephritis. C1q is composed of 18 polypeptide chains which include 6 A-chains, 6 B-chains, and 6 C-chains. Each chain contains an N-terminal collagen-like region and a C-terminal C1q globular domain. Alternate splicing results in multiple transcript variants.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human C1QA (AA: 96-245) expressed in E. Coli.
Butyrophilin is the major protein associated with fat droplets in the milk. It is a member of the immunoglobulin superfamily. It may have a cell surface receptor function. The human butyrophilin gene is localized in the major histocompatibility complex (MHC) class I region of 6p and may have arisen relatively recently in evolution by the shuffling of exons between 2 ancestral gene families
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human BTN1A1 (AA: extra 27-242) expressed in E. Coli.
Butyrophilin is the major protein associated with fat droplets in the milk. It is a member of the immunoglobulin superfamily. It may have a cell surface receptor function. The human butyrophilin gene is localized in the major histocompatibility complex (MHC) class I region of 6p and may have arisen relatively recently in evolution by the shuffling of exons between 2 ancestral gene families
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human BTN1A1 (AA: extra 27-242) expressed in E. Coli.
This gene encodes a protein that belongs to the pro-apoptotic subfamily within the Bcl-2 family of proteins. The encoded protein binds to Bcl-2 and possesses the BH3 domain. The protein directly targets mitochondria and causes apoptotic changes, including loss of membrane potential and the release of cytochrome c.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human BNIP3L (AA: 1-187) expressed in E. Coli.
This gene was identified by its translocation in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. The protein encoded by this gene contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB. This protein is reported to interact with other CARD domain containing proteins including CARD9, 10, 11 and 14, which are thought to function as upstream regulators in NF-kappaB signaling. This protein is found to form a complex with MALT1, a protein encoded by another gene known to be translocated in MALT lymphoma. MALT1 and this protein are thought to synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to the same pathogenetic process that leads to the malignancy. Alternative splicing results in multiple transcript variants.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human BCL10 (AA: 98-234) expressed in E. Coli.
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. The association and the ratio of BAX to BCL2 also determines survival or death of a cell following an apoptotic stimulus. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Dec 2019]
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human BAX(AA: (13-160)) expressed in E. Coli.
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. The association and the ratio of BAX to BCL2 also determines survival or death of a cell following an apoptotic stimulus. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human BAX expressed in E. Coli.
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. The association and the ratio of BAX to BCL2 also determines survival or death of a cell following an apoptotic stimulus. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human BAX expressed in E. Coli.
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. The association and the ratio of BAX to BCL2 also determines survival or death of a cell following an apoptotic stimulus. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Dec 2019]
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human BAX (AA:(13-160)) expressed in E. Coli.
This gene encodes a protein belonging to the B7 costimulatory protein family. Proteins in this family are present on the surface of antigen-presenting cells and interact with ligand bound to receptors on the surface of T cells. Studies have shown that high levels of the encoded protein has been correlated with tumor progression. A pseudogene of this gene is located on chromosome 20. Multiple transcript variants encoding different isoforms have been found for this gene.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human B7H4 (AA: extra 25-259) expressed in HEK293 cells.
This gene encodes a protein belonging to the B7 costimulatory protein family. Proteins in this family are present on the surface of antigen-presenting cells and interact with ligand bound to receptors on the surface of T cells. Studies have shown that high levels of the encoded protein has been correlated with tumor progression. A pseudogene of this gene is located on chromosome 20. Multiple transcript variants encoding different isoforms have been found for this gene.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human B7H4 (AA: extra 25-259) expressed in HEK293 cells.
The autosomal dominant cerebellar ataxias (ADCA) are a heterogeneous group of neurodegenerative disorders characterized by progressive degeneration of the cerebellum, brain stem and spinal cord. Clinically, ADCA has been divided into three groups: ADCA types I-III. ADCAI is genetically heterogeneous, with five genetic loci, designated spinocerebellar ataxia (SCA) 1, 2, 3, 4 and 6, being assigned to five different chromosomes. ADCAII, which always presents with retinal degeneration (SCA7), and ADCAIII often referred to as the `pure' cerebellar syndrome (SCA5), are most likely homogeneous disorders. Several SCA genes have been cloned and shown to contain CAG repeats in their coding regions. ADCA is caused by the expansion of the CAG repeats, producing an elongated polyglutamine tract in the corresponding protein. The expanded repeats are variable in size and unstable, usually increasing in size when transmitted to successive generations. The function of the ataxins is not known. This locus has been mapped to chromosome 6, and it has been determined that the diseased allele contains 40-83 CAG repeats, compared to 6-39 in the normal allele, and is associated with spinocerebellar ataxia type 1 (SCA1). Alternative splicing results in multiple transcript variants, with one variant encoding multiple distinct proteins, ATXN1 and Alt-ATXN1, due to the use of overlapping alternate reading frames.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human ATXN1 (AA: 645-815) expressed in E. Coli.
The autosomal dominant cerebellar ataxias (ADCA) are a heterogeneous group of neurodegenerative disorders characterized by progressive degeneration of the cerebellum, brain stem and spinal cord. Clinically, ADCA has been divided into three groups: ADCA types I-III. ADCAI is genetically heterogeneous, with five genetic loci, designated spinocerebellar ataxia (SCA) 1, 2, 3, 4 and 6, being assigned to five different chromosomes. ADCAII, which always presents with retinal degeneration (SCA7), and ADCAIII often referred to as the `pure' cerebellar syndrome (SCA5), are most likely homogeneous disorders. Several SCA genes have been cloned and shown to contain CAG repeats in their coding regions. ADCA is caused by the expansion of the CAG repeats, producing an elongated polyglutamine tract in the corresponding protein. The expanded repeats are variable in size and unstable, usually increasing in size when transmitted to successive generations. The function of the ataxins is not known. This locus has been mapped to chromosome 6, and it has been determined that the diseased allele contains 40-83 CAG repeats, compared to 6-39 in the normal allele, and is associated with spinocerebellar ataxia type 1 (SCA1). Alternative splicing results in multiple transcript variants, with one variant encoding multiple distinct proteins, ATXN1 and Alt-ATXN1, due to the use of overlapping alternate reading frames.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human ATXN1 (AA: 645-815) expressed in E. Coli.
The protein encoded by this gene is a serine/threonine kinase and DNA damage sensor, activating cell cycle checkpoint signaling upon DNA stress. The encoded protein can phosphorylate and activate several proteins involved in the inhibition of DNA replication and mitosis, and can promote DNA repair, recombination, and apoptosis. This protein is also important for fragile site stability and centrosome duplication. Defects in this gene are a cause of Seckel syndrome 1.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human ATR (AA: 366-550) expressed in E. Coli.
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human ATG4B (AA: 1-221) expressed in E. Coli.
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human ATG4B (AA: 1-221) expressed in E. Coli.
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human ATG4A (AA: 258-398) expressed in E. Coli.
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human ATG4A (AA: 258-398) expressed in E. Coli.
The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD14 (AA: 230-345) expressed in E. Coli.
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contributes to the clonal expansion, survival, and development of T cells. It can also induce proliferation in peripheral monocytes, enhance T cell apoptosis induced by TCR/CD3 triggered activation, and regulate CD28 co-stimulation to promote Th1 cell responses. The expression of this receptor is induced by lymphocyte activation. TRAF adaptor proteins have been shown to bind to this receptor and transduce the signals leading to activation of NF-kappaB.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD137 (AA: 214-255) expressed in E. Coli.
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contributes to the clonal expansion, survival, and development of T cells. It can also induce proliferation in peripheral monocytes, enhance T cell apoptosis induced by TCR/CD3 triggered activation, and regulate CD28 co-stimulation to promote Th1 cell responses. The expression of this receptor is induced by lymphocyte activation. TRAF adaptor proteins have been shown to bind to this receptor and transduce the signals leading to activation of NF-kappaB.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD137 (AA: 214-255) expressed in E. Coli.
The protein encoded by this gene is an important signaling component of many interleukin receptors, including those of interleukin -2, -4, -7 and -21, and is thus referred to as the common gamma chain. Mutations in this gene cause X-linked severe combined immunodeficiency (XSCID), as well as X-linked combined immunodeficiency (XCID), a less severe immunodeficiency disorder.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD132 (AA: extra 23-262) expressed in E. Coli.
The protein encoded by this gene is an important signaling component of many interleukin receptors, including those of interleukin -2, -4, -7 and -21, and is thus referred to as the common gamma chain. Mutations in this gene cause X-linked severe combined immunodeficiency (XSCID), as well as X-linked combined immunodeficiency (XCID), a less severe immunodeficiency disorder.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD132 (AA: extra 23-262) expressed in E. Coli.
The protein encoded by this gene is the common beta chain of the high affinity receptor for IL-3, IL-5 and CSF. Defects in this gene have been reported to be associated with protein alveolar proteinosis (PAP).
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD131 (AA: extra 17-149) expressed in E. Coli.
The protein encoded by this gene is the common beta chain of the high affinity receptor for IL-3, IL-5 and CSF. Defects in this gene have been reported to be associated with protein alveolar proteinosis (PAP).
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human CD131 (AA: extra 17-149) expressed in E. Coli.
Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human ATG10 (AA: 1-125) expressed in E. Coli.
This gene encodes a subunit of the asialoglycoprotein receptor. This receptor is a transmembrane protein that plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins with exposed terminal galactose or N-acetylgalactosamine residues. The asialoglycoprotein receptor may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery. The asialoglycoprotein receptor is a hetero-oligomeric protein composed of major and minor subunits, which are encoded by different genes. The protein encoded by this gene is the less abundant minor subunit. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human ASGR2 (AA: 80-311) expressed in E. Coli.
Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human ARG1 (AA: (1-322)) expressed in E. Coli.
Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
Product Type:
Antibodies Primary
Storage Temp:
4°C -20°C for long term storage
Immunogen:
Purified recombinant fragment of human ARG1 (AA: (1-322)) expressed in E. Coli.
Histamine is an important messenger molecule and neuromodulator with four known G protein-coupled receptors (GPCRs), H1, H2, H3 and H4. Histamine receptor H3 (HRH3) is a presynaptic autoreceptor that regulates histamine release from histaminergic neurons via negative feedback [1] as well as a heteroreceptor that regulates the release of other neurotransmitters [2,3,4]. It is also expressed postsynaptically in the modulation of dopamine signalling.
HRH3 is expressed predominantly in the cortex, thalamus, hypothalamus, hippocampus, amygdala and basal ganglia. Dysregulated central histaminergic signalling has been associated with a number of psychiatric disorders including sleep disorders, ADHD, Alzheimer's disease, Parkinson's disease and schizophrenia.
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal Antibody
Format:
100 µg in 100 µl Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
Storage Temp:
Store at -20°C. Avoid freeze / thaw cycles.
Host Animal:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Expected Species:
Guinae Pig, Cat
Immunogen:
A synthetic peptide from the C-terminal region of human HRH3
If you would like further information regarding the immunogen used in the production of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
[1] Morisset S, Rouleau A, Ligneau X, Gbahou F, Tardivel-Lacombe J, Stark H, Schunack W, Ganellin CR, Schwartz JC, Arrang JM. High constitutive activity of native H3 receptors regulates histamine neurons in brain. Nature. 2000; 408:860-4. [2] Schlicker E, Betz R, Gothert M. Histamine H3 receptor-mediated inhibition of serotonin release in the rat brain cortex. Naunyn Schmiedebergs Arch Pharmacol. 1988; 337:588-90. [3] Schlicker E, Fink K, Hinterthaner M, Gothert M. Inhibition of noradrenaline release in the rat brain cortex via presynaptic H3 receptors. Naunyn Schmiedebergs Arch Pharmacol. 1989; 340:633-8. [4] Schlicker E, Fink K, Detzner M, Gothert M. Histamine inhibits dopamine release in the mouse striatum via presynaptic H3 receptors. J Neural Transm Gen Sect. 1993; 93:1-10.
153-4D9 reacts with human CD84 (Mw 74 kDa). CD84 is expressed on mature B-cells and on Bcell line but not on plasma cell lines. Immunohistochemistry demonstrated that is strongly stains tissue macrophages. It is also expressed on platelets and at low levels on blood T-cells. It is a highly N-glycosylated protein and belongs to immunoglobulin superfamily. It may play a role in leukocyte activation but cellular expression does not significantly increase after activation. 153- 4D9 was clustered at the Vth WLDA.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
153-4D9
Concentration:
100 ug/ul
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Schlossman SF et al. (eds) Leukocyte Typing V. p.699-700, Oxford University Press, Oxford, (1995)
References 2:
De la Fuente, M, et al, Blood 90(6): 2398-2405 (1997)
152-1D5 reacts with human CD84 (Mw 74 kDa). CD84 is expressed on mature B-cells and on Bcell lines but not on plasma cell lines. Immunohistochemistry demonstrated that it strongly stains tissue macrophages. It is also expressed on platelets and at low levels on blood T-cells. It is a highly N-glycosylated protein and belongs to immunoglobulin superfamily. It may play a role in leukocyte activation but cellular expression does not significantly increase after activation. 152- 1D5 was clustered at the Vth WLDA.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
152-1D5
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Schlossman SF et al. (eds) Leukocyte Typing V. p.699-700, (1995)
References 2:
De la Fuente, M, et al, Blood 90(6): 2398-2405 (1997)
CDw78 (also called Ba antigen, Leu21 or LO panB a) is present on some immature and some mature B-cells. The antigen appears on B-cell progenitors preceding CD10, CD19, CD22, and CD37. It is expressed on resting B-cells and reappears and persists in the cytoplasm and on the cell surface until cytoplasmic Ig appears. Its expression is greatly increased after B-cell activation in vitro. It is also found on tissue macrophages and on epithelial cells, but not on T-cells, NK cells, monocytes, granulocytes, thymocytes or bone marrow stromal fibroblasts nor myeloid tissues. 60-3G2 was typed at CD workshop IV.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
60-3G2
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Pinchouk VG. et al, Anticancer Research 8: 1377-1380 (1988)
References 2:
Gluzman DF. et al, Tissue Antigens 33: 151 (1989)
References 3:
Sidorenko SP. et al, Neoplasma 39: 3-9 (1992)
References 4:
Moldenhauer et al, Leucocyte Typing IV, pp 155 162, (1989)
References 5:
Pezzuto et al, Leucocyte Typing IV, pp 165 174, (1989)
NK-1 reacts with CD57, a 110 kDa antigen present on the surface of human NK-cells and Tcell subsets. In adult peripheral blood 20% of mononuclear cells are positive. Other positive tissues include peripheral nerves (myelin-associated glycoprotein) and prostate. Anti-CD57 also stains neuroendocrine cells and their derived tumors, including carcinoid tumor and medulloblastomas. Anti-CD57 can also be useful in separating type B3 thymoma from thymic carcinoma when combined with a panel that includes antibodies against GLUT1, CD5, and CEA.
IPO-24 reacts with CD37, a B lineage restricted antigen, strongly expressed throughout B-cell ontogeny from pre B-cell until plasmablasts. Plasma cells are negative. IPO-24 reacts with human B-cell lines Daudi, Raji, Namalva, Ramos and CABIL, whereas T-cell lines CEM, Molt-4, Jurkat, MT-4, H9 and non-lymphoid cell lines MeWo and A-431 are negative. IPO-24 stains Bcell follicles and the most strongly labelled elements are mantle zone lymphoid cells. Neutrophils, macrophages, thymocytes and dendritic reticulum cells are negative.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
IPO-24
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
G. Moldenhauer G. et al., CD37 Workshop Panel report in: Schlossman et al., Leucocyte Typing V, B9, 544-545, (1995)
References 2:
Sidorenko S.P. et al., J. Tumor Marker Oncology 5: 232 (1990)
References 3:
Sidorenko S.P. et al., Haemat. Transfusiol. 35 N4: 19-22 (1990)
1.3.3.22 Reacts with human CD81 (TAPA-1), a 24 kDa member of the TM4 superfamily of proteins with four transmembrane domains. MAbs to CD81 have been shown to have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas. CD81 can associate with CD37 and/or CD53, or on the surface
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
1.3.3.22
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Kishimoto T. et al., eds. Leukocyte Typing VI, Garland Publishing, (1997)
CD28, a glycoprotein of 44-88 kDa, is the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. While 204-12 remained unclustered at the VIth International Workshop on human leukocyte differentiation antigens, the antibody has later been verified as CD28 specific.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
204-12
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Kishimoto T. et al., eds. Leukocyte Typing VI, Garland Publishing, (1997)
Moon-4 is specific for CD29, an adhesion receptor and costimulatory molecule. This antigen was initially characterized as gpIIa on platelets and as the common ?1 subunit of the very late antigen (VLA) protein family. CD29 forms a non-covalent heterodimeric complex with integrin ? subunits. It is also mediates adhesion to invasin and thrombospondin.
Antibody Isotype:
IgG1,kappa
Monosan Range:
MONOSAN
Clone:
Moon-4
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Deaglio, S. et. al, J Immunol 160(1): 395-402 (1998)
RIV12 reacts with CD1b, a 44KDa type 1 glycoprotein associated with beta2-microglobulin. It is expressed on dendritic cells, Langerhans cells, thymocytes and T acute lymphoblastic leukemia cells. CD1, type 1 membrane protein, has structural similarity to the MHC class 1 molecule and has been shown to present lipid antigens for recognition by T lymphocytes. CD1b is also expressed in Langerhans interdigitating cells.
143-44 recognizes a protein of 32 kDa, identified as CD8a. The CD8 molecule consists of ? and ?-chains, which are disulphide-linked into heterodimers or homodimers. CD8 is expressed on a T-cell subset (cytotoxic/suppressor T-cells), thymocytes and NK-cells. The majority of CD8+ T-cells express CD8 as heterodimer. Some subpopulation of CD8+ T-cells as well as NK-cells may express homodimer. CD8 functions as a co-receptor in concert with TCR for binding the MHC class I/peptide complex. The HIV-2 envelope glycoprotein binds CD8 ?-chain but not ?-chain. 124-1D1 was assigned at the IVth International Workshop.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
143-44
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Knapp, W. et. al. Leucocyte Typing IV, p1076, Oxford Univ. Press (1989)
IBL-3/25 is directed against the ?-chain of CD8. The CD8 complex consists of a disulphide-linked ?/? heterodimer with a MW of 30 kDa or an ?/? homodimer with a MW of 32 kDa. The CD8 molecule binds to HLA class I molecules during interaction of CD8+ T-cells with antigenpresenting cells or with target cells. CD8+ T-cells include most of the cytotoxic T-cells.
Antibody Isotype:
IgG-K
Monosan Range:
MONOSAN
Clone:
IBL-3/25
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Knapp W. et. al. Leukocyte Typing IV, p342-343, (1989)
References 2:
Parnes JR, Semin Immunol 6: 221-229 (1994)
References 3:
Delon J. et al. Immunity 9(4): 467-73 (1998)
References 4:
Akimoto H, et al. Immunology 95(2): 214-218 (1998)
EBS-CD-007 recognized a protein of 32 kDa, identified as CD8b. The CD8 molecule consists of ? and ? chains, which are disulphide-linked into heterodimers or homodimers. CD8 is expressed on a T-cell subset (cytotoxic-suppressor T-cells), thymocytes and NK cells. The majority of CD8+ T-cells express CD8 as heterodimer. Some subpopulation of CD8+ T-cells as well as NKcells may express homodimer. CD8 functions as a co-receptor is concert with TCR for binding the MHC class I/peptide complex. HIV-2 envelope glycoprotein binds CD8 ? chain but not ? chain.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-007
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Knapp W. et. al. Leukocyte Typing IV, p342-343, Oxford University Press, 1989
References 2:
Parnes JR, Semin Immunol 6: 221-229 (1994)
References 3:
Hadi Hossein Nataj Arab et al., Gastroenterol Hepatol Bed Bench. 8(2): 132139 (2015)
EBS-CD-045 reacts with human CD100, a 150 kDa homodimer cell-surface antigen that is expressed on resting and PHA-stimulated T-cells. It is absent from bone marrow, erythrocytes, eosinophils and endothelial cells. The protein is weakly expressed on NK-cells, EBV transformed B-cells, monocytes and tumor T-cell lines. It plays a role in homotypic cell adhesion and in T-cell activation.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-045
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Hall K, et al. P. Natl. Acad. Sci. USA 93: 11780 (1996)
References 2:
Mizrahi S, et al. PLoS One. 2(9): e818 (2007).
References 3:
Yoshino N, et al.. Exp. Anim. (Tokyo) 49: 97 (2000)
CD109 is a GPI-anchored member of the alpha-2-macroglobulin (A2M) and complement family of proteins. It is expressed on activated T-cells, platelets, hematopoietic stem cells, megakaryocyte precursors, vascular endothelial cells, basal and myoepithelial cells of secretory glands, and squamous cell carcinomas. A 170-180 kDa precursor is autocatalytically reduced to 150 kDa and 120 kDa forms. On keratinocytes CD109 binds TGF-beta and associates with TGFbeta RI and TGF-beta RII, resulting in inhibition of TGF-beta signalling. Polymorphisms of CD109 include the platelet-specific Gov antigen and the blood group ABH antigens. Alloantibodies directed against these antigens result in unsuccessful platelet transfusions, neonatal alloimmune thrombocytopenia, and post-transfusion purpura.
EBS-CD-060 reacts with a peptide epitope on the extracellular domain of human Glycophorin A, a 39 kDa sialoglycoprotein, present on red cells and erythroid precursor cells. Glycophorin A is the carrier of blood group M and N specificities, while Glycophorin B carries S and U specificities. Providing a mucin like coat, Glycophorin may play a role in preventing red cell aggregation in the circulation. Glycophorin also acts as receptors for Sendai and Parvovirus
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-060
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Cartron JP et al, Transfus Med Rev 6(2): 63-92 (1992)
Ep-CAM (also called ESA, EGP40, 17-1A antigen, KSA, GA7333-2) is a 40 kDa epithelial protein expressed on baso-lateral cell surfaces in very many epithelial tissues (but absent from mesothelial tissues). The 324AA have 3 potential glycosylation sites and is a transmembrane glycoprotein. The extracellular domain has a cysteine-rich repeat and a small domain with homology to nidogen. It is a homophilic cell-cell adhesion molecule (Ep-CAM). EBS-CD-061 reacts with most epithelial cells and carcinomas.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-061
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Edwards DP et. al. Cancer Res 46:1306-17 (1986)
References 2:
Litvinov et al. J. Cell. Biol. 125: 437-446 (1994)
203-6 Reacts with human CD27, a disulphide-linked 120 kDa dimer. CD27 is a lymphocyte-specific member of the TNF-R/NGF-R superfamily, and is expressed on a subset of human thymocytes and on the majority of mature T-lymphocytes. CD27 is highly expressed on activated T and B-cells. 203- 6 was clustered at the VIth WLDA.
Antibody Isotype:
IgG3-K
Monosan Range:
MONOSAN
Clone:
203-6
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Kishimoto T. et al., eds. Leukocyte Typing VI, p509-514, Garland Publishing, Inc, (1997)
CD36 is a 80-90 kDa protein, expressed on platelets, monocytes and macrophages, microvascular endothelial cells, erythrocyte precursors, mammary epithelial cells, and some macrophage derived dendritic cells. CD36 acts as a receptor for thrombospondin (TSP), collagen types I, IV and V, P.falciparum malaria-infected erythrocytes, and sickle erythrocytes. CD36 plays a role in platelet aggregation, macrophage foam cell development, inflammation, and the tissue ischemia observed in sickle cell disease and cerebral malaria.
EBS-T-232 reacts with SITTTE in the VNTR domain of human MUC3. The mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats (VNTRs) encoded by 60 base pairs (MUC1), 69 base pairs (MC2) and 51 base pairs (MUC3). Cancer cells of colon, breast and stomach, normal cells of salivary gland, breast, lung, and gastrointestinal tract are positive for MUC3.
Antibody Isotype:
IgG2b-K
Monosan Range:
MONOSAN
Clone:
EBS-T-232
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Apostolopoulos, V. et al. J. Gastroenterol. Hepatol. 1995; 10 (5): 555-561
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in Human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 7.8. The individual Human Cytokeratins are numbered 1 to 20. The various epithelia in the Human body usually express Cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used in the distinction of different types of epithelial malignancies. The Cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. RCK105 reacts exclusively with Cytokeratin 7 which is present in a subgroup of glandular epithelia and their tumors, as well as transitional epithelium and transitional carcinoma.
EBS-CD-044 reacts with CD99 or MIC2. Human thymocytes, PBLs and some T-ALL isolates and cell lines are positive. It is also present on pancreas and on Ewing sarcoma, which forms the practical application of the antibody. It is involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes.
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-044
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Rajasekariah et al. in: Leukocyte Typing III A. McMichael (ed), Oxford University Press, Oxford (1987)
VU-3C6 reacts with the protein core of MUC1, an apical cell side epithelial marker which is upregulated or switched on in the majority of carcinomas. The dominant epitope of VU-3- C6 is GVTSAPDTRPAP, located in the VNTR domain of MUC1. Binding of VU-3C6 is enhanced after glycosylation of the DTR motif.
VU-3D1 reacts with the protein core of MUC1, an apical cell side epithelial marker which is upregulated or switched on in the majority of carcinomas. The dominant epitope of VU3D1 is SAPDTRPA, located in the VNTR domain of MUC1.
VU-4H5 reacts with the protein core of MUC1, an apical cell side epithelial marker which is upregulated or switched on in the majority of carcinomas. The dominant epitope of VU-4H5 is PDTR, located in the VNTR domain of MUC1. In tissue sections, VU-4H5 also displays prominent staining of the cytoplasm.
VU-11D1 reacts with the protein core of MUC1, an apical cell side epithelial marker which is upregulated or switched on in the majority of carcinomas. The dominant epitope of VU11D1 is TSAPDTRP, located in the VNTR domain of MUC1. Binding of VU-11D1 is enhanced after glycosylation of the DTR motif.
VU-11E2 reacts with the protein core of MUC1, an apical cell side epithelial marker which is upregulated or switched on in the majority of carcinomas. The dominant epitope of VU11E2 is TSAPDTRP, located in the VNTR domain of MUC1. Binding of VU-11E2 is enhanced after glycosylation of the DTR motif
VU-12E1 reacts with the protein core of MUC1, an apical cell side epithelial marker which is upregulated or switched on in the majority of carcinomas. The dominant epitope of VU-12- E1 is PDTRPAP, located in the VNTR domain of MUC1. Binding of VU-12E1 is enhanced after glycosylation of the DTR motif
VU-13F11 reacts with the protein core of MUC1, an apical cell side epithelial marker which is upregulated or switched on in the majority of carcinomas. The dominant epitope of VU-13F11 has not yet been determined but is located in the VNTR domain of MUC1 (confirmed by ELISA).
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
VU-13F11
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Schol D. et al, Tumor Biol. 19(Suppl 1): 35-45 (1998)
VU-2G7 reacts with the protein core of MUC1, an apical cell side epithelial marker which is upregulated or switched on in the majority of carcinomas. The dominant epitope of VU2G7 includes the PDTR motif, located in the VNTR domain of MUC1. Binding of VU-2G7 is significantly enhanced when the threonine of the PDTR motif bears a GalNAc
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
2G7
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Ryuko, K. et al. Tumor Biol. 21(4): 197-210 (2000)
References 2:
Karsten, U. et al. Cancer. Res. 58(12): 2541-2549 (1998)
EBS-C-002 reacts with NuMA or Nuclear Mitotic Apparatus protein, which at the onset of mitosis redistributes from the nucleus to two centrosomal structures at the poles of the mitotic spindle, where it plays a vital role in establishing and maintaining its bipolar structure. After anaphase the protein redistributes from the spindle polar region into the reforming nucleus and concentrates initially at the site where nuclear lamins and perichomatin have been reported to assemble. In contrast to mitotic cells, post-mitotic neurons display NuMA both in the nucleus and in the cytoplasm. Due to release from dead cells, NuMA is also used as oncological marker in serum and urine. In addition, chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 has been detected in patients with acute promyelocytic leukemia.
1-13M1 recognizes the peptide core of gastric mucin M1/MUC5AC), and more specifically with the a epitope, which is the most abundant amongst the a, b, c, d, e, f, and h protein core epitopes defined by Bara for M1. MUC5AC is present in primary ovarian mucinous cancer and gastric cancer, but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification pancreatic carcinoma and precancerous changes vs. normal pancreas.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
1-13M1
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bara, J. et al., Cancer Res.46: 3983-3989 (1986)
References 2:
Bara, J. et al., Biochem. J. 254: 185-193 (1988)
References 3:
Bara, J. et al., Int. J. Cancer 47: 304-310 (1991)
References 4:
Bara, J. et al., J. Immunol. Methods 149: 105-113 (1992)
References 5:
Guyonnet Duperat V. et al., Biochem. J. 305: 211 219 (1995)
2-11M1 recognizes the peptide core of gastric mucin M1/MUC5AC, and more specifically with the b epitope amongst the a, b, c, d, e, f, g, and h protein core epitopes defined by Bara for M1. 2-11M1 and 9-13M1 react exclusively with epitopes located in the N-terminal cysteine-rich part of the peptide core MUC5AC. MUC5AC is present in primary ovarian mucinous cancer and gastric cancer, but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
2-11M1
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bara, J. et al., Cancer Res.46: 3983-3989 (1986)
References 2:
Bara, J. et al., Biochem. J. 254: 185-193 (1988)
References 3:
Bara, J. et al., Int. J. Cancer 47: 304-310 (1991)
References 4:
Bara, J. et al., J. Immunol. Methods 149: 105-113 (1992)
References 5:
Guyonnet Duperat V. et al., Biochem. J. 305: 211 219 (1995)
2-12M1 recognizes the peptide core of gastric mucin M1/MUC5AC, and more specifically with the c epitope amongst the a, b, c, d, e, f, g, and h protein core epitopes defined by Bara for M1. 2-12M1 and 45M1 both specifically react with epitopes located in the Cterminal cysteine rich part of the peptide core of gastric mucin (MUC5AC). MUC5AC is present in primary ovarian mucinous cancer and gastric cancer, but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. AntiMUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
2-12M1
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bara, J. et al., Cancer Res.46: 3983-3989 (1986)
References 2:
Bara, J. et al., Biochem. J. 254: 185-193 (1988)
References 3:
Bara, J. et al., Int. J. Cancer 47: 304-310 (1991)
References 4:
Bara, J. et al., J. Immunol. Methods 149: 105-113 (1992)
References 5:
Guyonnet Duperat V. et al., Biochem. J. 305: 211 219 (1995)
45M1 recognizes the peptide core of gastric mucin M1 (now: MUC5AC), and more specifically with the h epitope amongst the a, b, c, d, e, f, g and h protein core epitopes defined by Bara for M1. 45M1 and 2-12M1 both specifically react with epitopes located in the C-terminal cysteine rich part of the peptide core of MUC5AC. MUC5AC is present in primary ovarian mucinous cancer and gastric cancer, but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
45M1
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bara, J. et al., Int. J. Cancer 47: 304-310 (1991)
References 2:
Bara, J. et al., J. Immunol. Methods 149: 105-113 (1992)
58M1 recognizes the peptide core of gastric mucin M1 (now: MUC5AC), and more specifically with the e epitope amongst the a, b, c, d, e, f, g and h protein core epitopes defined by Bara for M1. MUC5AC is present in primary ovarian mucinous cancer and gastric cancer, but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
58M1
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bara, J. et al., Cancer Res.46: 3983-3989 (1986)
References 2:
Bara, J. et al., Biochem. J. 254: 185-193 (1988)
References 3:
Bara, J. et al., Int. J. Cancer 47: 304-310 (1991)
References 4:
Bara, J. et al., J. Immunol. Methods 149: 105-113 (1992)
References 5:
Guyonnet Duperat V. et al., Biochem. J. 305: 211 219 (1995)
9-13M1 recognizes the peptide core of gastric mucin M1/MUC5AC), and more specifically with the d epitope amongst the a, b, c, d, e, f, g and h protein core epitopes defined by Bara for M1. 9-13M1 and 2-11M1 react exclusively with epitopes located in the the Nterminal cysteine-rich part of the peptide core MUC5AC. MUC5AC is present in primary ovarian mucinous cancer and gastric cancer, but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification pancreatic carcinoma and pre-cancerous changes vs. normal pancreas
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
9-13M1
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bara, J. et al., Cancer Res.46: 3983-3989 (1986)
References 2:
Bara, J. et al., Biochem. J. 254: 185-193 (1988)
References 3:
Bara, J. et al., Int. J. Cancer 47: 304-310 (1991)
References 4:
Bara, J. et al., J. Immunol. Methods 149: 105-113 (1992)
References 5:
Guyonnet Duperat V. et al., Biochem. J. 305: 211 219 (1995)
Skp2 (S-phase Kinase-associated Protein 2) belongs to the family of F-box proteins that interact with the Cyclin A-Cdk2 complex. Skp2 is essential for the G1-S transition in both transformed cells and diploid fibroblasts. Biochemical and genetic experiments have demonstrated that Skp2 is required for the ubiquitination and consequent degradation of p27 in cultured mammalian cells and in vitro reconstitution assays. In normal tissues, Skp2 is expressed in tonsil and placenta.
202-36 Reacts with human CD26 (Dipeptidyl peptidase IV), a 110 kDa protein which occurs in the cell membrane of T-lymphocyte and is upregulated after activation. CD26 is also present on endothelial cells and also expressed on activated B-cells and natural killer cells and abundantly on epithelia. CD26 is implicated in a variety of biological functions including T-cell activation, cell adhesion with extracellular matrix such as fibronectin or collagens, and in HIV infection. Crosslinking of CD26 using this antibody dramatically enhances the anti-CD3-induced IL-2 production. In Western blotting, this mAb reacts with only glycosylated CD26, but not with deglycosylated froms. It does not prevent ADA binding to CD26. The mAb was clustered at the VIth WLDA.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
202-36
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Kishimoto T. et al., eds. Leukocyte Typing VI, Garland Publishing, (1997)
Bra4F1 reacts with CD15 (220 kDa). CD15 is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. CD15 is further expressed in Reed-Sternberg cells in classic Hodgkins disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes. It is also expressed on a wide variety of other tumor cells including myeloid leukemia, breast, colorectal, and lung cancer cells. Bra4F1 was clustered at the IVth International Workshop on Leucocyte Differentiation Antigens.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
BRA4F1
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Chorvath B. et al., Neoplasma 36(6), 633-642 (1989)
References 2:
Leukocyte typing IV, Oxford Univ. Press, 868, 870 and 877 (1989)
87-6F9 Reacts with CD11a, a transmembrane molecule with 1145 amino acid residues and a MW of 180 kDa. CD11a is expressed on lymphocytes, granulocytes, monocytes and macrophages. Levels on memory T-cells tend to increase. CD11a plays a key role in mediating leukocyte adhesion to endothelium during inflammatory response through binding to ICAM 1 (CD54). Other ligands are ICAM-2 and ICAM-3. It is also involved in many other T-cell functions and immune phenomena. When paired with CD18, it forms the integrin alphaLbeta2 adhesion. 87-6F9 Potently blocks LFA-1 dependent homotypic cell aggregation and was typed in the IVth International Leucocyte Typing Workshop
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
87-6F9
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Knapp et al. (Eds.), Leukocyte Typing IV, 1989 : sections N1 (pp. 543.551)
References 2:
Petruzzelli L et al. J Immunol 155(2): 854-866 (1995)
References 3:
Edwards CP et al. J Biol Chem 273(44): 28937-44 (1998)
References 4:
Shang XZ et al,. Eur J Immunol 28(6): 1970-1979 (1998)
CB-T6 specifically reacts with CD1a, an MHC like transmembrane glycoprotein of 49 kDa, that presents lipid molecules and is non-covalently associated with beta2-microglobulin. The antigen is strongly expressed on cortical thymocytes and dendritic cells and disappears at later stages of maturation.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
CBT6
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Crispe N. et al. Immunol. Today 17: 522-525 (1996)
References 2:
Yakimchuk et al. Eur J Immunol. 41(3): 694705 (2011). doi:10.1002/eji.201040808
CB-30 reacts with CD66e or CEA with MW of 80-200 kDa. CEA is present in fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Ant-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbladder, urachus, salivary gland, ovary, and endocervix.
C3 has been characterized in the ISOBM TD-2 workshop and assigned by K. Nunstad to a group of antibodies with low affinity to human alpha fetoprotein. Human alpha fetoprotein is an oncofetal protein of 70 kDa and expressed in fetal liver but normally absent in healthy adult tissues. It is expressed in all yolk sac tumors, in some other germ cell tumors and in hepatocellular carcinomas.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
C3
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Tsung K., et al. J. Immunol. Methods 39: 363-368 (1980)
References 2:
Michell B.et al. Eur. J. Cancer Clin. Oncol. 19: 1239-1246 (1983)
References 3:
Yazova A.K. et al. Immunol. Lett. 25: 325-330 (1990)
References 4:
Nustad K. Et al. Tumor Biol 19: 293 -300 (1998)
References 5:
Yakimenko E.F. et al. Tumor Biol 19: 301-309 (1998)
D10 has been characterized in the ISOBM TD-2 workshop and assigned by K. Nustad to group D of a cluster of 6 major epitopes of human alpha fetoprotein. Human alpha fetoprotein is an oncofetal protein of 70 kDa. It is expressed in fetal liver and is normally absent in healthy adult tissues. It is positive on all yolk sac tumors, on some other germ cell tumors and on hepatocellular carcinomas.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
D10
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Tsung K., et al. J. Immunol. Methods 39: 363-368 (1980)
References 2:
Michell B.et al. Eur. J. Cancer Clin. Oncol. 19: 1239-1246 (1983)
References 3:
Yazova A.K. et al. Immunol. Lett. 25: 325-330 (1990)
References 4:
Nustad K. Et al. Tumor Biol 19: 293 -300 (1998)
References 5:
Yakimenko E.F. et al. Tumor Biol 19: 301-309 (1998)
Cris-3 reacts with CD11a, a transmembrane molecule with 1145 amino acid residues and a MW of 180 kDa. CD11a is expressed on lymphocytes, granulocytes, monocytes and macrophages. Levels on memory T-cells ten to increase. CD11a plays a key role in mediating leukocyte adhesion to endothelium during inflammatory responses through binding to ICAM-1 (CD54). Other ligands are ICAM-2 and ICAM-3. It is also involved in many other T-cell functions and immune phenomena. When paired with CD18, it forms the integrin alphaLbeta2 heterodimer also called LFA-1. LFA-1 is chiefly responsible for lymphocyte adhesion.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
CRIS-3
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Reinherz et. al., EL. Leukocyte Typing II. New York: Springer-Verlag (1985)
Hyb-8 recognizes free biotin and biotinylated proteins (HS biotin, NHS amidocaproylbiotin). The length of the spacer does not affect mAb binding. The antibody also react with biotinylated DNA probes.
AFT14 reacts with Aflatoxin B1 and B2, a 55 kDA protein secreted by Aspergillus. The aflatoxins are a group of closely related mycotoxins that are widely distributed in nature. The most important of the group is aflatoxin B1 (AFB1), which has a range of biological activities, including acute toxicity, teratogenicity, mutagenicity and carcinogenicity. In order for AFB1 to exert its effects, it must be converted to its reactive epoxide by the action of the mixed function mono-oxygenase enzyme systems (cytochrome P450- dependent) in the tissues (in particular, the liver) of the affected animal. This epoxide is highly reactive and can form derivatives with several cellular macromolecules, including DNA, RNA, and protein. Cytochrome p450 enzymes may additionally catalyse the hydroxylation (to AFQ1 and AFM1) and demethylation (to AFP1) of the parent AFB1 molecule, resulting in products less toxic than AFB1. Conjugation of AFB1 to glutathione (mediated by glutathione Stransferase) and its subsequent excretion is regarded as an important detoxification pathway in animals. Aflatoxins are well recognized as a cause of liver cancer, but they have additional important toxic effects. Aflatoxin B1 is a potent hepatocarcinogenic and mutagenic mycotoxin of Aspergillus flavus.
Antibody Isotype:
IgG2a,kappa
Monosan Range:
MONOSAN
Clone:
AFT14/CBL03
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
F S Chu and I Ueno, Appl Environ Microbiol 33(5): 11251128 (1977)
References 2:
Groopman, JD. et al, Proc. Natl. Acad. Sci. USA 81: 7728-7731 (1984)
The neurofilament (NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. RNF403 reacts exclusively with the phosphorylated isoform of the160 kD neurofilament protein.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
RNF403
Concentration:
1 mg/ml
Storage buffer:
PBS with 0.09% sodium azide
Storage:
2-8°C
References 1:
Bauwens et al. Ann Otol Rhinol Laryngol 1992;101:479-486
In lung cancer diagnosis Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is sofar not found in Human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non-SCLC (cell cultures).RNL-4 reacts with peripheral nerves and ganglia of various tissues and cross-reacts with smooth muscle cells and myoepithelium. In the central nervous system it reacts with the neurohypophysis and pars intermedia of the pituitary gland, and a weak diffuse staining was observed in neurons of the granular and molecular layer of the cerebellar cortex, while glial cells, cerebellar medulla and Purkinje cells are negative. Reticulon-1 has been found to indicate neuronal differentiation and to be downregulated in neurological pathologies.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
RNL-4
Concentration:
1 mg/ml
Storage buffer:
PBS with 0.09% sodium azide
Storage:
2-8°C
References 1:
Senden et al. Eur J Cell Biol 1996;69:197-213
References 2:
Senden et al. J Pathol 1997;182:13-21
References 3:
Hens et al. Cell Tissue Res 1998;292:229-237
References 4:
Kim et al. Biochem Biophys Res Comm 2000;276:329-334
In lung cancer diagnosis Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is sofar not found in Human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non-SCLC (cell cultures).RNL-2 recognizes an epitope located within the region of amino acids 421-589 of the neuro-endocrine specific protein Reticulon-1A (NSP-A), which is also present in the N-terminal part of Reticulon-1B (NSP-B). In normal tissues, RNL-2 reacts with brain Purkinje cells, pancreatic islet cells, cells in the pituitary gland and some (peripheral) nerve fibers. In addition, a few epithelia show positive staining.
In lung cancer diagnosis Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is sofar not found in Human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non-SCLC (cell cultures).RNL-2 recognizes an epitope located within the region of amino acids 421-589 of the neuro-endocrine specific protein Reticulon-1A (NSP-A), which is also present in the N-terminal part of Reticulon-1B (NSP-B). In normal tissues, RNL-2 reacts with brain Purkinje cells, pancreatic islet cells, cells in the pituitary gland and some (peripheral) nerve fibers. In addition, a few epithelia show positive staining.
Smoothelin is a constituent of the smooth muscle cell (SMC) cytoskeleton. Antibodies directed to smoothelin are useful tools to monitor SMC differentiation. Smoothelin is exclusively expressed in fully differentiated (contractile) SMCs. RNA and protein analyses revealed a broad species distribution of this protein. Smoothelin has also been detected in smooth-muscle neoplasms. Cells with SMC-like characteristics, such as myofibroblasts and myoepithelial cells, as well as skeletal and cardiac muscle do not contain smoothelin. Confocal scanning laser microscopy of tissue sections and cells in culture show a filamentous organization of smoothelin colocalizing with actin stress fibers. In immunoblots two molecular weight isoforms are detected i.e. a 59 kDa isoform specific for visceral SMC (smoothelin A), and an isoform with a molecular weight of approximately 100 kDa in vascular SMC (smoothelin B). Human smoothelin is encoded by a single copy gene which is loCated on chromosome 22.
Pen 9 reacts mainly with the thiazolidin ring of penicillin, but not with the lactam ring. The nature of the side chain in the penicilloyl group does not affect antibody binding as was shown by testing Pen 9 against benzylpenicillin, ampicillin, amoxillin and 6-aminopenicillanic acid. The presence of carrier protein is not essential for the presentation of the antigen associated with Pen 9.
Antibody Isotype:
IgG1-kappa
Monosan Range:
MONOSAN
Clone:
Pen 9
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
P. de Haan et al., Int. Archs Allergy appl. Immun. 76: 42-46 (1985)
PN-15 reacts with a lectin receptor like glycoprotein of 200 kDa (gp200), present in proximal renal tubules and on urothelium. The antigen is carbohydrate in nature. Other normal tissues that display the antigen include breast, parathyroid glands, thymus and epididymis. Among renal carcinomas 93% of primary and 84% of metastatic carcinomas are positive. Bladder cancers are also largely positive. Other tumor types include breast cancer, teratocarcinomas and parathyroid adenomas. The antigen, also called DEC-205, was assigned to CD205 at CD workshop VII. In the immune system it can facilitate tolerance to self-antigens through uptake of apoptosis derived material by dendritic cells, which in turn present fragments through MHC II and MHC I, either inducing or repressing immune responses, depending on the nature of concomitant signals.
Antibody Isotype:
IgG2b-kappa
Monosan Range:
MONOSAN
Clone:
PN-15
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Yoshida, S.O. et al, Cancer Res 49: 1802-1809 (1989)
References 2:
Li, G, et al, Anticancer Res. 20(4): 2773-8 (2000)
References 3:
Batchelder C.A. et al, Anat Rec (Hoboken) 297(8): 1392-1406 (2014)
References 4:
Cykowski M.D. et al, Ultrastruct Pathol 39(1): 69-77 (2015)
AE-2 recognizes double stranded DNA expressed in the nucleus of eukaryotes. Dilution advice: Flow cytometry (1-2 µg/million cells in 0,1 ml, fix cells in 4% PFA for 10 min, at 4°C, permeabilize with 0,2% saponin or digitonin for 15 min, at 4°C). ? Immunofluorescence (1-2 µg/ml). ? Immunohistology (1-2 µg/ml for 30 min at RT; staining of formalin-fixed tissues requires incubating tissue sections in 4N HCl, for 30 minutes).
Antibody Isotype:
IgG3
Monosan Range:
MONOSAN
Clone:
AE-2
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Epstein, AL et al. In Progress on nonhistone protein research. 1: 117-137 (1987)
FR4D11 reacts with high affinity to CD10 or CALLA, a cell surface enzyme with neutral metalloendopeptidase activity, inactivating a variety of biologically active peptides. CD10 is a 100 kDa glycoprotein, expressed on 70% of pre-B ALL-cells (common ALL), but also on early lymphoid progenitor-cells in bone marrow and fetal liver. Other normal CD10 positive tissues include renal epithelium, fibroblasts and germinal centre B-cells. Density of CD10 antigen has been shown to be related to cell differentiation and may have prognostic value for B-cell lineage acute leukemia. CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
FR4D11
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Brown, B. et al., J. Natl. Canc. Inst,.55: 1281-1289 (1975)
References 2:
Tran-Paterson, R. et al., Blood, 76: 775-782 (1990)
References 3:
Doerken, B. et al., in Knapp, W. et. al. (eds)., Leucocyte Typing IV, Oxford Univ. Press, pp 33-34
Integrin ?M (also designated complement component receptor 3 ?-chain; CD11b (p170); macrophage antigen ? polypeptide; cell surface glycoprotein Mac-1 ?-subunit; CR3 ?-chain; MAC1A; MO1A and ITGAM) is a cell adhesion molecule that acts as a receptor for cell surface ligands such as intracellular adhesion molecules (ICAMs) or soluble ligands. Integrins are heterodimeric proteins that contain an ?-chain and a ?-chain. Integrin ?M combines with Integrin ?2 (CD18) to form a leukocyte-specific integrin referred to as macrophage receptor-1 (Mac-1) or inactivated-C3b (iC3b) receptor 3 (CR3). Integrin ?M-?2 is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-010
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Beekhuizen H, et al., J Immunol. 145(2):510-8 (1990)
References 2:
Argenbright LW et al., J Leukoc Biol. 49(3):253-7 (1991)
References 3:
Zhou L, et al. J Biol Chem. 269(25):17075-9 (1994)
References 4:
Miller LJ, et al. J Immunol. 137(9):2891-900 (1986)
Integrin ?X (CD11c, leukocyte surface antigen p150/95, CR4, Axb2) is a type 1 transmembrane protein that traditionally combines with ?2 chain to form a leukocyte-specific integrin known as inactivated-C3b (iC3b) receptor 4 (CR4). Integrin ?X/?2 shares similar properties of the Integrin ?M/?2 in mediating adherence of neutrophils and monocytes to stimulated endothelial cells and in phagocytosis of complement coated particles. Abnormal expression of Integrin ?X is characteristic of hairy cell leukemia (HCL) and is dependent upon activation of proto-oncogenes Ras and JunD. Integrin ?x is present on dendritic cells, macrophages and NK-cells. Upon activation, DCs present in skin (Langerhans cells_, lining of nose, lung, stomach, intestine and blood can migrate to lymphoid tissues and interact with T and B-cells to initiate and shape the immune response.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-011
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Cabañas C, et al., Hybridoma 7(2):167-76 (1988)
References 2:
Cabañas C, et al., Immunol Lett. 20(3):193-76 (1988)
References 3:
Zhou JQ, et al. Blood 82:800-6 (1993)
References 4:
Nicolaou, F., et al. Blood 101: 4033-4041 (2003)
References 5:
Edwards, A.D. et al. J. Immunol. 171: 47-60 (2003)
EBS-CD-12 recognizes an extracellular epitope on an integral membrane glycoprotein of 150 kDa, identified as CD13 (also known as aminopeptidase-N). CD13 is present on most cells of myeloid origin including granulocytes, monocytes, mast cells, and GM-progenitor cells. It is also expressed by the majority of AML, CML in myeloid blast crisis, and in a smaller fraction of lymphoid leukemias. CD13 is also present on fibroblasts; endothelial cells, epithelial cells from renal proximal tubules and intestinal brush border, bone marrow stromal cells, osteoclasts, and cells lining bile duct canaliculi. CD13 plays a role in metabolism of biologically active peptides, in phagocytosis, and in bactericidal/tumoricidal activities. It also serves as a receptor for human coronaviruses (hCoV) and human cytomegalovirus (hCMV).
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-012
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Favaloro EJ, et al., Exp Hematol. 13:1695-701 (1993)
References 2:
Favaloro EJ, et al., Clin Chim Acta.220(1):81-90 (1993)
References 3:
Lachance C., et al. J Virol. 72(8):6511-9. (1998)
References 4:
Koch AE, et. al. Am J Pathol. 138(1): 165-73.( 1991)
References 5:
Principe, S. et al, Proteome Res. 6;11(4): 2386-96 (2012)
EBS-CD-013 specifically recognizes CD16. This molecule also named low affinity Fc-receptor for IgG (FcgammaRIII) exhibits two truncated Ig-like domains and is 50-80 kDa. It is highly expressed on NK-cells, granulocytes and macrophages. EBS-CD-013 binds to 15% peripheral lymphocytes of healthy donors (NK-cells), granulocytes, macrophages. CD16 represents the functional receptor structure for antibody-dependent cellular cytotoxicity.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-013
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Deaglio S. et al., Blood 99(7): 2490-8 (2002)
References 2:
Zilber MT et al. Proc Natl Acad Sci U S A 97(6): 2840-5 (2000)
References 3:
Wirthmueller U et al. J Exp Med. 175(5): 1381-90 (1992)
CB16 specifically recognizes CD 16. This molecule also named low affinity Fc-receptor for IgG (FcgammaRIII) exhibits two truncated Ig-like domains and is 50-80 kDa. It is highly expressed on NK-cells, granulocytes and macrophages. CB16 binds to 15% peripheral lymphocytes of healthy donors (NK-cells), granulocytes, macrophages. CD16 represents the functional receptor structure for antibody-dependent cellular cytotoxicity
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
CB16
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Deaglio S. et al., Blood 99(7): 2490-8 (2002)
References 2:
Zilber MT et al. Proc Natl Acad Sci U S A 97(6): 2840-5 (2000)
References 3:
Wirthmueller U et al. J Exp Med. 175(5): 1381-90 (1992)
CDw17 is an intermediate glycosphingolipid from the metabolism of higher gangliosides that localizes to sphingolipid-sterol rafts. CDw17 is found on monocytes, granulocytes, basophils, platelets, a subset of peripheral B-cells (CD19+) and tonsil dendritic cells. It is rapidly down regulated on activated granulocytes and is upregulated on IL-2 activated T-lymphocytes. CDw17 binds to bacteria and may function in phagocytosis. It may also be involved in angiogenesis. Aberrant levels of glycosphingolipids are a feature of cancer cells and may influence integrin clustering and internalization.
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-014
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Knapp W. Leukocyte Typing IV, Oxford Univ. Press, (1989)
It recognizes a transmembrane glycoprotein of 95 kDa, identified as CD18 or intergrin-2 (Workshop III). It complexes non-covalently with either L, M, or X integrin (CD11a, b, or c) to form the heterodimers. LFA-1, MAC-1, and p150,95, respectively. LFA-1 is the receptor for three members of the Ig supergene family of proteins, ICAM-1 (CD54, ICCAM-2 (CD102), and Mac-1 and p150,95 bind to ICAM-1, fibrinogen, and IC3b. ICAM-3 (CD50). CD18/CD11 heterodimeric molecules are involved with cell/cell and cell/extracellular adhesion in immune and inflammatory responses. This Mab blocks these cellular interactions. 68-5A5 was clustered at the IIIrd International Workshop on human leucocyte differentiation antigens.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
68-5A5
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
McMichael A.J.et al., Leucocyte typing III, Oxford University Press, Oxford, (1987)
CB19 is specific for the antigen CD19. This antigen has a MW of 120 kDa and contains a 280 residue extracellular domain and a 240 residue cytoplasmic domain. It is a critical signal transduction molecule that regulates B-lymphocyte development, activation, and differentiation. It plays a dominant role in establishing signalling thresholds for antigen receptors and other surface receptors on B-lymphocytes. This antigen is lost upon terminal differentiation to plasma cells.
RIV12 reacts with CD1b, a 44KDa type 1 glycoprotein associated with beta2-microglobulin. It is expressed on dendritic cells, Langerhans cells, thymocytes and T acute lymphoblastic leukemia cells. CD1, type 1 membrane protein, has structural similarity to the MHC class 1 molecule and has been shown to present lipid antigens for recognition by T lymphocytes. CD1b is also expressed in Langerhans interdigitating cells.
CD2 is a transmembrane glycoprotein that is expressed on peripheral blood T lymphocytes, NK cells and thymocytes. Interaction between CD2 and its counter receptor LFA3 (CD58) on opposing cells optimizes immune system recognition, thereby facilitating communication between helper T lymphocytes and antigen presenting cells, as well as between cytolytic effectors and target cells. EBSCD-003 reacts with human T-cells, leukemic T-cells and T-cell lines. EBS-CD-003 also reacts with some, if not all, E-RFC-receptors on K and NK cells.
Antibody Isotype:
IgG2b-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-003
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Thurlow PJ, et al., transplantation 36: 293-298 (1983)
References 2:
Kozarsky KF, et al, Cell Immunol 150: 235-246 (1993)
References 3:
Schlossman SF et al. Leukocyte Typing?V Oxford?University Press:?342-352 (1995)
109-3C2 binds with CD20 which is a 30/33 kDa non-glycosylated transmembrane phosphoprotein with three extensive hydrophobic regions. CD20 is involved in regulation of B-cell activation. It is expressed on the surface of all B-cells beginning at the pro-B phase (CD45R+, CD117+) and progressively increasing in concentration until maturity. Plasma cells are negative. CD20 is retained on many B-cell malignancies. CD20 positive cells are also sometimes found in cases of Hodgkins disease, myeloma, and thymoma. 109-3C2 has been clustered at IVth and Vth HLDA Workshops.
Antibody Isotype:
IgG3-K
Monosan Range:
MONOSAN
Clone:
109-3C2
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Knapp W. Leukocyte Typing IV, Oxford Univ. Press, (1989)
References 2:
Schlossman SF et al. Leukocyte Typing?V Oxford?University Press:?342-352 (1995)
93-1B3 binds with CD20 which is a 30/33 kDa non-glycosylated transmembrane phosphoprotein with three extensive hydrophobic regions. CD20 is involved in regulation of Bcell activation. It is expressed on the surface of all B-cells beginning at the pro-B phase (CD45R+, CD117+) and progressively increasing in concentration until maturity. Plasma cells are negative. CD20 is retained on many B-cell malignancies. CD20 positive cells are also sometimes found in cases of Hodgkins disease, myeloma, and thymoma. 93-1B3 has been clustered at the IIIrd and Vth HLDA Workshops.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
93-1B3
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Cobbold, S. et al., in leucocyte typing III, Oxford University Press (1987)
References 2:
Schlossman SF et al. Leukocyte Typing?V Oxford?University Press:?342-352 (1995)
FR5A10 reacts with CD21, a 140 kDa cell surface molecule which acts as a receptor for EBV, for human complement factor C3d (CR2) and for IFN-alpha. It is a glycoprotein, made up of multiple (n=15) Short Consensus Repeats (S.C.R.) sequences. FR5A10 has been assigned to (S.C.R.) sequences. FR5A10 has been assigned to S.C.R. numbers 5-8. FR5A10 is highly specific to CR2 and shows no cross-reaction with CR1. CD21 is expressed strongly on mature B-cells, follicular dendritic cells and weakly on immature thymocytes and T-lymphocytes. In B-cell ontogeny, CD21 appears after the preB-stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells. CD21 expression is also gradually lost after stimulation of B-cells in vitro.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
FR5A10
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Schlossman SF et al. Leukocyte Typing?V Oxford?University Press:?342-352 (1995)
References 2:
Aubry JP et al. Leukocyte Typing V, p535-536, Oxford University Press, Oxford, (1995)
FR10B4 reacts with high affinity to CD22, which is expressed in the cytoplasma of all B-cells, appearing as early as cell-surface CD19 during B-cell development. Its present on the surface of most mature sIg+ B-cells with especially high expression on hairy cell and prolymphocytic leukemia cells. CD22 is a member of the immunoglobulin super-family and acts as an adhesion molecule: BL-CAM. On frozen sections, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, while germinal centre B-cells react relatively weakly.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
FR10B4
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Campana, D., et al.Leucocyte Typing IV, Oxford Univ. Press, (1989), pp 190-192
EBS-CD-017 reacts with CD25 (55 kDa) which associates as alpha chain with CD122 and the common gamma chain (CD132) to form the high-affinity IL-2 receptor complex. With respect to lymphomas, CD25 is present on malignant cells of Hodgkins disease, HTLV-1+ adult T-cell leukemia, cutaneous T-cell lymphoma, and hairy cell leukemia. Increased levels of soluble CD25 are observed in leukemias/lymphomas and inflammatory/ autoimmune diseases. CD25 alone appears to function as a low affinity receptor and associates with CD122 (IL-2R chain, p75) and CD132 (common chain) to form the high affinity IL-2 receptor complex. CD25 antibodies detect three epitope regions, A, B and C. EBS-CD-017 recognizes B epitope, which is located at residue 3-104 of CD25 and does not block IL-2 binding to CD25. CD25 antibodies have been used successfully as a carrier for cytotoxic drugs enabling specific delivery to IL-2RA displaying target cells.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-017
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Yamamura T. et al, Eur J Surg 168(1): 49-54 (2002)
References 2:
Lundin K. et al, Anal Biochem 299(1): 92-7 (2001)
References 3:
Raivio E. et al, APMIS 105(2): 108-14 (1997)
References 4:
Boutin B. et al, Neuropediatrics 20(4): 202-6 (1989)
B-B12 reacts with 5 invariable CD3-chains: CD3y or gp26, CD3d or gp20, CD3e or gp20, CD3f or p16 (homodimer), CD3n or p28. Molecular mass: 25-28, 20 and 16 kDa. The main reactivity is with T cells, including thymocytes, mature T cells and T cell lines.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
B-B12
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Meuer SC et al, Nature 303(5920): 808-810 (1983)
References 2:
Reinherz et al, Cell 30: 715 (1982)
References 3:
Borst et al, J. Biol. Chem. 258: 5135 (1983)
References 4:
Van den Elsen et al, Nature 312 (5993): 413-8 (1984)
FS12 reacts with CD31 (PECAM-1), a 130-140 kDa member of the immunoglobulin gene superfamily that is expressed on cells of the vasculature, including platelets, endothelial cells, myeloid cells and certain lymphocyte subsets.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
FS12
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Sandilands, G. et al. Clin. and Exp. Immunology, 162(3): 516-27 (2010)
References 2:
Kishimoto T. et al., eds. Leukocyte Typing VI, Garland Publishing, Inc, (1997)
CD33 is a 67 kDa glycoprotein of the sialic acid-binding immunoglobulin-like lectin (SIGLEC) family, displaying the immune-receptor tyrosine-based inhibitory motif (ITIM), able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies. ITIMs are also used for ubiquitinmediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. CD33 is expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. It is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-022
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Knapp W. Leukocyte Typing IV, Oxford Univ. Press, (1989)
References 2:
Favaloro EJ et al, Dis Markers 5(4): 215-25 (1987)
References 3:
Favaloro EJ et al, Br J Haematol 69(2): 163-71 (1988).
EBS-CD-025 reacts with a 45 kDa glycopeptide which is a type II membrane glycoprotein with a transmembrane sequence near the regulation of lymphocyte adhesion to endothelial cells. Its ligand is CD31. CD38 is found on early cells of B and T lineages and on activated B- and tcells. It is not found on most mature resting peripheral lymphocytes. Also positive are thymus cells and Ig secreting plasma cells. The CD34+ and CD38- population of hematopoietic stems cells defines the most pluripotent cells (e.g. blast colony forming cells). EBS-CD-025 antibody blocks the EBS-CD-026 epitope, and vice versa.
EDU-2 reacts with human CD4, a 55 kDa single chain transmembrane glycoprotein that contains four extracellular immunoglobulin-like domains and is mainly expressed on T-helper lymphocytes, but also on cortical thymus cells, microglial and dendritic cells. It binds to HLA class II molecules during the interaction of CD4+ T cells with antigen-presenting cells or target cells. CD4 also serves as the primary cellular receptor for human immunodeficiency virus (HIV). EDU-2 was assigned to CD4 at the International Leucocyte Typing Workshops II and IV.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
EDU-2
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Knapp W. Leukocyte Typing IV, Oxford Univ. Press, (1989)
References 2:
Reinberz EL et al. eds. Leykcocyte Typing II, Springer-Verlag, Berlin, (1985)
ACTH (same as corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. 2F6 is specific to synacthen (aa 1-24 of ACTH); does not react with CLIP (aa 17-39 of ACTH). ACTH is also produced by cells of the immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
2F6
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Kimitsuki K. et al. J Vet Med Sci 76(1) 133-138 (2014).
84-3C1 reacts with a 95/115 kDa protein on T-cells and thymocytes and a 115/135 kDa molecule on neutrophils and platelets. 70-90% of T-cell lymphomas and from 22-37% of Bcell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a Blineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma. In addition, expression is altered in Wiskott Aldrich syndrome. A proportion of AIDS patients have antibodies to CD43. A soluble form called galactoglycoprotein is present in serum. 85-3C1 was typed at the 3rd International Workshop on Human Leucocyte Differentiation antigens.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
84-3C1
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Cobbold, S. et al. In Leucocyte typing III, Oxford University Press, pp 789-803 (1987)
References 2:
Stross, WP. et al. J. Clin. Path. 42: 953-961 (1989)
145-23 reacts with CD44, a type 1 transmembrane glycoprotein providing cell-cell and cellmatrix adhesion while linked to the cytoskeleton. It is involved in haematopoiesis, lymphocyte homing and activation, and tumor metastasis. It binds to fibrin, hyaluronate and other matrix proteins. On tumors CD44H is highly expressed, suggesting an important role in progression for this isoform. CD44 also forms the protein backbone of the human erythrocyte Lutheran antigen system. The epitope of 145-23 is resistant to (chemo)trypsin digestion.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
145-23
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Nishina S. et al., Graefes Archiv. Clin. Exp. Opthalm. 235: 92-96 (1997)
References 2:
Horny H.P. et al. Virchows Arch. 429: 91-94 (1996)
CD45 glycoprotein have various molecular weight on various cell types: B-cells 240 kDa, thymocytes 180 kDa, T-cells multiple bands. Reduced in PAGE gels: 180 and 240 kDa. Isoforms are produced by alternative splicing of domains 4, 5 and 6. Various isoform are expressed differently on different lymphocytes. All hematopoietic cells express CD45 proteins except erythrocytes. Relevant epitopes are termed CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45R or CD45R0 (exon 4-6 spliced out).
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
Bra55
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Chorváth et al., Neoplasma 34(6), 685-692, (1987)
References 2:
Chorváth et al., Neoplasma 35(5), 495-501, (1988
References 3:
Chorváth et al. Leukocyte Typing IV, pp. 634-637, (1989)
EBS-CD-028 reacts with CD46 or Membrane Cofactor Protein (MCP; 52-74 kDa) All nucleated human cells carry CD46, which has multiple common protein isoforms of 52 kDa to 66 kDa, and 74 kDa on placental cells and some transformed cells. CD46 acts as a cofactor for complement factor I, a serine protease, which protects autologous cell against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. It may further be involved in the fusion of the spermatozoa with the oocyte during fertilization. CD46 acts as a co-stimulatory factor for T-cells, which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity. A number of viral and bacterial pathogens seem to exploit this property and directly induce an immunosuppressive phenotype in T-cells by binding to CD46.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-028
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Iwata, K., et al. J. Biol. Chem. 270: 15148-15152 (1995)
References 2:
Liszewski, M.K., et al. Adv. Immunol. 61: 201-283 (1996)
References 3:
Liszewski, M.K., et al. J. Immunol. 156: 4415-4421 (1996)
Cris-1 reacts with a 67 kDa protein, consistent with human CD5. Cris-1 was assigned at the Ist and IIIrd International Leucocyte Typing Workshops. CD5 is a pan T-cell marker that also reacts with a Bcell marker subset and a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10%) of diffuse large Bcell lymphoma. CD5 aberrant expression is useful in the diagnosis of mature T-cell neoplasms.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
Cris-1
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Proceedings of the first international workshop and conference on human leukocyte differentiation antigens. Oxford University Press, Oxford (1983).
References 2:
Leukocyte Typing III, McMichael A. J. et al. (Eds.), Oxford University Press, Oxford (1987)
References 3:
Alberola-Ila J, et al, J Immunol. 148(5): 1287-93 (1992)
References 4:
Arrizabalaga P. et al, Nephron 53: 41-49 (1989)
References 5:
Guarne A. et al, Protein Science 5: 167-169 (1996)
101-1D2 reacts with the cellular adhesion molecule CD50 (ICAM-3), a single chain polypeptide with a MW of 12 kDa. The protein is heavily glycosylated and resistant to phosphatidylinositol phospholipase C treatment so probably not PO-anchored. 101-1D2 was provisionally clustered as CDw50 at the IV and confirmed as CD50 at the V international workshop on human leucocyte differentiation antigens.
5H4-E2 reacts with hCG beta chain, which is specific for hCG. The alpha chain is identical among hCG, TSH, FSH and LH. While hCG is secreted in large quantities by normal trophoblasts, it is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. Besides trophoblastic tumors e.g. choriocarcinoma, large cell carcinoma, adenocarcinoma and squamous cell carcinoma of the lung are also positive in 90%, 60% and 20% of cases respectively. In hCG producing tumors, including also certain testicular and embryonic carcinomas, the beta chain is produced in higher quantities than the alpha or dimeric chains. hCG expression by non-trophoblastic tumors may indicate aggressive behavior.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
5H4-E2
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
McDonald EA et. al. Endocrinology 150:4358-65 (2009)
BM-5 is specific marker for human myeloid cells and an early marker of myeloid differentiation. It recognizes a nuclear and cytoplasmic antigen present in granulocytes, monocytes, and myeloid precursor cells. It also reacts with a subset of myeloid leukemia cells. BM-5 has no reactivity with any other cell type in human tissues.
BM-4 recognizes a nuclear antigen expressed in human granulocytes (83%) monocytes (20%) and myeloid precursor cells residing in lymphoid and non-lymphoid tissues. BM-4 is an early marker of myeloid differentiation. It also reacts with a subset of myeloid leukemia cells. BM-4 has no reactivity with any other cell type in human tissues.
Induction studies using HL-60 cells showed that BM-3 identifies a nuclear antigen which is expressed during the early phases of myeloid differentiation, making BM-3 an early marker of myeloid differentiation. It is found in 98% of human granulocytes, in 80% of human monocytes and in myeloid precursor cells, residing in lymphoid and non-lymphoid tissues. It also reacts with a subset of myeloid leukemia cells. BM-3 has no reactivity with any other cell type in human tissues. In experiments using S-35 methionine labeled human myeloid leukemia cells BM-3 immunoprecipitated a 13 kDa protein.
CD53 is a 33-55 kDa protein expressed on monocytes and macrophages, dendritic cells, osteoblasts and osteoclasts, and on B- and T-cells from every stage of differentiation but is absent from platelets, red blood cells. CD53 appears to be the marker with widest reactivity as well as the marker with the strictest specificity to hematopoietic cells. 161-2 Partially inhibits T-cell proliferation induced by CD3 UCHT-1 antibody. 161-2 was typed in Kobe, Japan at the VIth International Workshop on human leucocyte differentiation antigens.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
161-2
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Kishimoto T. et al., eds. Leukocyte Typing VI, p509-514, Garland Publishing, Inc, (1997)
100-1A5 reacts with CD1b, a 44KDa type 1 glycoprotein associated with beta2-microglobulin. It is expressed on dendritic cells, Langerhans cells, thymocytes and T acute lymphoblastic leukemia cells. CD1, type 1 membrane protein, has structural similarity to the MHC class 1 molecule and has been shown to present lipid antigens for recognition by T lymphocytes. CD1b is also expressed in Langerhans interdigitating cells. 100-1A5 also reacts with pyramidal cells in the brain and was typed at the IVth International CD Workshop.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
100-1A5
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Knapp et. al., W (1989). Leucocyte typing IV. Oxford University Press
Until recently, immunological markers for myeloid cells have been lacking, especially those which identify different levels of cellular differentiation. The BM series provides a new panel of monoclonal antibodies which stain early precursor and mature forms of human myeloid cells. This panel of monoclonal antibodies reacts with antigenic determinants present in normal myeloid cells and leukemias of similar derivation. BM-2 recognizes a cytoplasmic antigen expressed in mature human granulocytes (polys) residing in lymphoid and non-lymphoid tissues. It does not react with any other cell type in human tissues.
BM-1 is reactive in B5 fixed, paraffin embedded tissue sections to human myeloid cells and derived malignancies. The antibody reacts with a 183 kDa cytoplasmic antigen with DNAbinding characteristics which is expressed in most myeloid precursor cells and myeloid leukemias. BM-1 is positive on myeloid precursors in bone marrow but lymph nodes are negative. Tissue granulocytes are positive as well as scattered cells in peripheral cortex and interlobular septae of adult and fetal thymus. Portal regions of fetal liver (18 weeks) are also positive
19-OLE reacts with H type 2 antigen, the basis of the ABO blood group system, involving three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity. It is expressed on endothelial cells, epithelial cells and granulocytes. Increased expression of this antigen has been observed on some tumor tissues such as gastric carcinomas, urothelial carcinomas, and colon carcinomas.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
19-OLE
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bara, J. et al. Blood transfusion and immunohaematology, Ph Rouger, D Anstee and Ch Salmon (Eds), Arnette, France 30 (5), p. 685-692, (1987)
References 2:
Marionneau, S. et al. Gasterenterology, 122: 1967-1877 (2002)
References 3:
Garcher, K. et al. Invest Ophthalmol Vis Sci. 35: 1184-1191 (1994)
CD147 is a transmembrane glycoprotein of the immunoglobulin superfamily. It is expressed more intensely on thymocytes than on mature peripheral blood T cells. CD147 is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. CD147 is involved in the regulation of matrix remodeling at the epidermal-dermal interface. It stimulates the production of interstitial collagenase, gelatinase A, stromelysin-1 and various metalloproteinases (MMPs) by fibroblasts. These enzymes, which are typically increased during tissue degradation and wound healing, are important factors in cancer invasion and metastasis.
A9E4 reacts with GnRH receptors in the anterior pituitary. GnRH stimulates the gonadotrophs of the anterior pituitary to secrete luteinising hormone (LH) as well as follicle-stimulating hormone (FSH). The receptor contains seven hydrophobic transmembrane domains connected by hydrophilic extracellular, and intracellular loops, characteristic of G-protein coupled receptors. Some cancers like ovarian and breast cancers sometimes carry GnRH receptors
Antibody Isotype:
IgG1,kappa
Monosan Range:
MONOSAN
Clone:
A9E4
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Karande, AA, et al, Molec. Cell. Endocrinol. 114: 51-56 (1995)
133-1C6 reacts with human CD100, a 100 kDa homodimer cell-surface antigen that is expressed on resting and PHA-stimulated T cells. It is absent from bone marrow, erythrocytes, eosinophils and endothelial cells. The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor T cell lines. It plays a role in homotypic cell adhesion and in T cell activation.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
133-1G6
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Knapp, W. et. al. (eds)., Leucocyte Typing IV, Oxford Univ. Press, pp 193-196 (1989)
References 2:
Kishimoto T et al. (eds) Leukocyte Typing VI, Garland Publishing, New York, (1997)
CD98 exists as a heterodimer containing a disulphide-linked glycosylated heavy chain and a nonglycosylated light chain. It is a member of the solute carrier family and encodes a cell surface, transmembrane protein. The protein exists as the heavy chain of a heterodimer, covalently bound through disulphide bonds to one of several possible light chains. The encoded transporter plays a role in regulation of intercellular calcium levels and transport L-type amino acids. Alternatively spliced transcript variants, encoding different isoforms, have been characterized. Monocytes express high levels of CD98 antigen. Peripheral blood T- and B-cells, as well as NKcells and granulocytes express low levels of CD98. Activation of T-cells and NK-cells leads to upregulation of CD98. RBCs are negative. IPO-T10 was typed at the VIth International Workshop and Conference on Human Leukocyte Differentiation Antigens
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
IPO-T10
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
The VIth International Workshop and Conference on Human Leukocyte Differentiation Antigens, Kobe, Japan (1996)
References 2:
Woodhead VE et al. Int Immunol.12(7):1051-61 (2000)
B-R18 specifically recognizes CD95. CD95 is a cell surface glycoprotein with a MW of 40-45 kDa and contains 8 kDa of N-glyosidic-linked polysaccharide. It is a receptor for TNFSF6/FASLG, a member of the nerve growth factor receptor/tumor necrosis factor superfamily, mediating receptor-triggered apoptosis. CD95 is preferentially expressed by the CD54RAlow and CD45ROhigh subset of memory T-cells, but it also found on peripheral monocytes. It is further found on human B-cell lines like pre-B cells, EBV infected cells, Burkitt cells and plasmacytoma cells. It also binds to human T-cell lines, myeloid cell lines, hepatocyte carcinoma and endothelial cells.
7LE reacts with Lewis a blood group antigen, a carbohydrate determinant present on both glycolipids and glycoproteins, expressed a.o. in colonic and kidney epithelial cells. Lewis a may be useful for detection of gastrointestinal, pancreatic and colorectal carcinomas.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
7LE
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Creuzot-Garcher et al. Invest Oph(thalmol Vis Sci. 40(8):1631-6 (1999)
References 2:
Torrado et al. Cancer Epidemiol Biomarkers Prev. 1(3): 199-205 (1992)
References 3:
Torrado et al. Gastroenterology, 102 (2): 424-430 (1992)
CD90 (Thy-1) is an 18-35 kDa GPI-anchored glycoprotein and a member of the immunoglobulin superfamily. It may contribute to inhibition of proliferation/differentiation of hematopoietic stem cells and neuron memory formation in the CNS. It consists of a single Ig domain (112 amino acids; 25-35 kDa) inserted into the cell membrane via a GPI-anchor. Expressed by hematopoietic stem cells and neurons in all species studied. Its highly expressed in connective tissue and various fibroblasts and stromal cell lines, expressed only on small % fetal thymocytes, 10-40% of CD34+ cells in bone marrow, and
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
AF-9
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Cervelló I et al. PLoS One 6: e21221 (2011)
References 2:
Lehmann GM et al. Am J Physiol Cell Physiol 299: C672-81 (2010)
2-25LE reacts with Lewis b antigen which is highly expressed in stomach, colon, small intestine, lung and kidney and to a lesser extent in salivary gland, bladder, uterus and liver
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
2-25LE
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bara et al. Cancer Res. 46: 3983-3898 (1986)
References 2:
Bara et al. Biochem. J. 254: 185-193 (1988)
References 3:
Creuzot-Garcher et al. Invest Oph(thalmol Vis Sci. 40(8):1631-6 (1999)
References 4:
Good et al. Vox Sanguinis 62(3): 180-189 (1992)
References 5:
Torrado et al. Gastroenterology, 102 (2): 424-430 (1992)
F4-31C2 reacts with CD54 or ICAM1 (Intercellular Adhesion Molecule 1). ICAM1 belongs to the immunoglobulin superfamily, C2 subset, is a transmembrane molecule of 90 kDa with 7 potential N-glycosylation sites. It is expressed on resting monocytes and endothelial cells and in response to inflammatory cytokines such as TNF-alpha, IL1 and IFN-gamma, can be highly upregulated on many other cells, e.g. on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrin CD11a/CD18 (LFA 1) and to CD1b/CD18 (Mac 1). The interaction of CD54 with LFA 1 enhances antigen specific T-cell activation. CD54 also binds to CD43, fibrinogen, most human rhinoviruses and to Plasmodium falciparum infected erythrocytes. ICAM1 may also be related to progression and metastasis of tumors.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
F4-31C2
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Johnson, J.P., et al., in Knapp, W., et al. Leucocyte Typing IV, pp 681-683 (1989)
This antibody reacts with the C-terminus of Nucleophosmin (B23). NPM1 (Nucleophosmin 1, B23, nutramin, NO38) is a ubiquitously expressed phosphoprotein involved in ribosome assembly/transport, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity, centrosome duplication, and regulation of p53. NPM1 continuously shuttles between the nucleus, cytoplasm, nucleolus and chaperoning core histones from the nucleus to the cytoplasm.
F4-29D9 reacts with CD55 or DAF (Decay Accelerating Factor). All leucocytes as well as human erythrocytes, fibroblasts, platelets, endothelial cells and neuroectodermal cells are positive for DAF. F4-29D9 also recognizes an antigen on spermatozoid cells. It is a glycosylphosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. CD55 also serves as receptor for CD97 and for echovirus and coxsackie B virus. DAF is deficient in both granulocytes and monocytes in patients with paroxysmal nocturnal haemoglobinuria.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
F4-29D9
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Kishimoto T. et al., eds. Leukocyte Typing VI, p509-514, Garland Publishing, Inc, (1997)
References 2:
B.E. Loveland - in Leucocyte Typing VI - Part 6 CD55 Workshop Panel Report pp519-520, (1997)
References 3:
Ruix-Delgado, GJ et al, Hematology 14: 33-7 (2009)
EBS-CD-033 reacts with an extracellular domain (close to the cell membrane) of CD56/NCAM. Only 2 isoforms of NCAM are recognized by EBS-CD-033 (180 kDa and 145 kDa). EBS-CD-033 was used successfully for immunoscintigraphy and immunotherapy of SCLC xenografts in nude mice. EBS-CD-033 recognizes NCAM in retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell lung carcinomas. NCAM is also expressed on some mesodermally derived tumors (a.o. rhabdomyosarcoma). Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-033
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Schol DJ. et al, Int. J. Cancer Suppl.2: 35-40 (1988)
References 2:
Kibbelaar RE. et al, Eur J Cancer 27(4): 431-5 (1991)
References 3:
Brezicka FT. et al, APMIS. 99(9): 797-802 (1991)
References 4:
Takaku H. et al. Am J Gastroenterol. 94(5): 1402-4 (1999)
References 5:
Kaufman O. et al, Hum. Pathol. 28(12): 1373-1378 (1997)
CD59, or protectin, is a 18-22 kDa cell surface molecule on an GPI anchor. It regulates complementmediated cell lysis and is supposed to protect normal and tumor cells from cytotoxic attack by homologous complement through binding to C8 and C9. CD59 is expressed on leucocytes, vascular epithelium, a variety of epithelial cells and placenta. B-cell express low levels. The expression of CD59 on erythrocytes is important for their survival. Genetic defects in GPI-anchor attachment, that cause a reduction or loss of CD59 and CD55 on erythrocytes produce the symptoms of the disease Paroxysmal nocturnal hemoglobinuria (PNH). 193-27 Was typed at the VIth International Workshop on human leucocyte differentiation antigens.
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
193-27
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Kishimoto T. et al., eds. Leukocyte Typing VI, p509-514, Garland Publishing, Inc, (1997)
References 2:
Shichishima T. et al. Br J Haematol, 85(2):378-386 (1993)
References 3:
Navenot JM. et al. Transfusion 38(4):337-342 (1998)
References 4:
Murray EW et al, J Biol Chem, 273(39):25279-25284 (1998)
EBS-CD-005 recognizes the CD6 molecule, a single chain transmembrane glycoprotein of 120 kDa, which is expressed on the majority of mature T-cells an mature thymocytes. A B-cell subset is weakly positive and B-CLLs may also be reactive. CD6 is a type 1 transmembrane glycoprotein that is tyrosine phosphorylated during TCR-mediated T-cell activation. CD6 shows significant homology to CD5. Antibodies to CD6 are used to deplete T-cells from bone marrow transplants to prevent graft versus host disease.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-005
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Yssel et al., Cell. Immunol. 105: 161 (1987)
References 2:
Kamoun et al., J. Immunol. 127: 987 (1981)
References 3:
Reinherz et al., Proc. Nat. Acad. Sci. 79: 6047 (1982)
EBS-CD-034 reacts with a complex of CD41 and CD61, i.e. alpha IIb integrin and the integrin beta chain. The MW of the GPIIIa is 105 kDa unreduced and 90 kDa reduced. The integrin beta 3 chain can also form a complex called the vitronectin receptor with integrin alpha V: the CD51/CD61 complex. Ligands are fibrinogen, fibronectin, von Willebrand factor, vitronectin and thrombospondin. Residues 237-248 of GPIIIa or CD61 are critical in adhesive protein binding. The CD51/CD61 complex is also found on endothelial cells, some B-cells, monocytes/macrophages and tumor cells.
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-034
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Burns et al., Cell 45, 269 280 (1986)
References 2:
McMichael AJ et al. (eds) Leukocyte Typing III, Oxford University Press, Oxford, (1987)
References 3:
Schlossman S. et al. (eds) Leukocyte Typing V, Oxford University Press (1995)
EBS-CD-036 reacts with CD63 and is mainly used in combination with EBS-CD-147 and/or anti-PEM (MUC1) to identify melanoma from carcinoma in paraffin sections. Melanomas are EBS-CD-036 and EBS-CD-147 positive, but PEM negative. EBS-CD-036 reacts in frozen sections with melanoma and breast cancers, smooth muscle and lung (weakly). In paraffin sections melanomas (primary skin, uveal and choroidal), melanoma metastases, clear cell CA, carcinoids, skin nevi, medullary CA of thyroid, prostate CA, some breast, ovary, lung, colorectal and bladder CA positive. Normal tissues that are positive include: mast cells, sweat glands, Islets of Langerhans, pituitary, pancreas, peribronchial glads, Paneth cells and prostate glands.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-036
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Vennegoor C. et al., Int. J. Cancer 35: 287-295 (1985)
References 2:
Palmer AA et al., Pathology 17: 335-339 (1985)
References 3:
Hagen EC et al., Histopathology 10: 689-700 (1986)
References 4:
Duffield, A., et al. Proc. Natl. Acad. Sci. USA 100: 15560-15565 (2003)
C2 has been characterized in the ISOBM TD-2 workshop and assigned by K. Nustad to group E of a cluster of 6 major epitopes of human alpha fetoprotein. Human alpha fetoprotein is an oncofetal protein of 70 kDa. It is expressed in fetal liver and is normally absent in health adult tissues. It is positive on all yolk sac tumors, on some other germ cell tumors and on hepatocellular carcinomas.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
C2
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Tsung K., et al. J. Immunol. Methods 39: 363-368 (1980)
References 2:
Michell B.et al. Eur. J. Cancer Clin. Oncol. 19: 1239-1246 (1983)
References 3:
Yazova A.K. et al. Immunol. Lett. 25: 325-330 (1990)
References 4:
Nustad K. Et al. Tumor Biol 19: 293 -300 (1998)
References 5:
Yakimenko E.F. et al. Tumor Biol 19: 301-309 (1998)
BF12 recognizes 40 kDa CD7, a member of the immunoglobulin gene superfamily and expressed on the majority of immature and mature T-lymphocytes, and T-cell leukemia. It is also found on natural killer cells, a small subpopulation of normal B-cells and on malignant B-cells. CD7 associates directly with phosphoinositol 3-kinase. CD7 ligation induces production of D-3 phosphoinositides and tyrosine phosphorylation.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
BF12
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Wang, MY et. al, Bone Marrow Transplant. 9(5): 319-23 (1992)
124-1D1 recognizes 40 kDa CD7, a member of the immunoglobulin gene superfamily and expressed on the majority of immature and mature T-lymphocytes, and T-cell leukemia. It is also found on natural killer cells, a small subpopulation of normal B-cells and on malignant B-cells. CD7 associates directly with phospoinositol and tyrosine phosphorylation. 124-1D1 was assigned at the IVth International Workshop.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
124-1D1
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Knapp, W. et. al. Leucocyte Typing IV, p541, 667-670, 1087, Oxford Univ. Press (1989)
The transferrin receptor is a type II membrane glycoprotein existing as a homodimer of 180- 190 kDa with interchain disculphide bonding. The ligand is the serum iron transport protein transferrin. CD71 is expressed weakly on all resting leucocytes but is upregulated on all cells upon activation, reflecting the iron dependence of proliferation. In other tissues CD71 is expressed on most dividing cells, but also strongly on brain endothelium and alveolar macrophage. CD71 expression can reflect clinical behaviour or response to therapy in a number of malignancies including leukemia, lymphoma and breast cancer.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-040
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Van de Rijn et al. Cytogenet.Cell Genet. 36: 525 (1983)
References 2:
Oudermans et al. Cancer. 58: 1252 (1986)
References 3:
Beguin Y, et al, Leukemia (12): 2019-25 (1993)
References 4:
Rittenhouse-Diakun K, et al. Photochem Photobiol. 61(5): 523-8 (1995)
CD80 is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is included by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation.
Antibody Isotype:
IgG2b-K
Monosan Range:
MONOSAN
Clone:
C80-99
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Peach, R.J., et al. J. Biol. Chem. 270: 21181-21187 (1995)
References 2:
Fargeas, C.A., et al. J. Exp. Med. 182: 667-675 (1995)
EBS-CD-42 reacts with CD86, a member of the immunoglobulin superfamily, and highly expressed on monocytes, dendritic cells and stimulated B-cells. It is probably the major CD28 ligand. Furthermore, EBS-CD-042 blocks binding of soluble CD152 to CD86.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-042
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Giguère JF et al, J Virol 78: 6222-32 (2004)
References 2:
Mauri D et al, J Immunol 155: 118-27 (1995)
References 3:
Schlossman S. et al. (eds) Leukocyte Typing V, Oxford University Press (1995)
References 4:
Kishimoto T. et al., eds. Leukocyte Typing VI, p509-514, Garland Publishing, Inc, (1997)
References 5:
Sandilands GP et al, Immunology 108: 329337 (2003)
RIV11 recognizes a protein of 32 kDa, identified as CD8a. The CD8 molecule consists of ? and ? chains, which are disulphide-linked into heterodimers or homodimers. CD8 is expressed on a T-cell subset (cytotoxic/suppressor T-cells), thymocytes and NK-cells. The majority of CD8+ T-cells express CD8 as heterodimer. Some subpopulations of CD8+ T-cells as well NK-cells may express homodimer. CD8 functions as a co-receptor in concert with TCR for binding the MHC class I/peptide complex. HIV-2 envelope glycoprotein binds CD8 ? chain but not CD8 ? chain.
EBS-CD-009 recognizes a protein of 32 kDa, identified as CD8a. The CD8 molecule consists of ? and ?chain, which are disulphide-linked into heterodimers or homodimers. CD8 is expressed on a T-cell subset (cytotoxic/suppressor T-cells), thymocytes and NK-cells. The majority of CD8+ T-cells express CD8 as heterodimer. Some subpopulation of SD8+ T-cells as well as NK-cells may express homodimer. CD8 functions as a co-receptor in concert with TCR for binding the MHC class I/peptide complex. HIV-2 envelope glycoprotein binds CD8 ?-chain but not ?-chain.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-009
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Knapp W. et. al. Leukocyte Typing IV, p342-343, (1989)
References 2:
Parnes JR, Semin Immunol 6: 221-229 (1994)
References 3:
Delon J. et al. Immunity 9(4): 467-73 (1998)
References 4:
Akimoto H, et al. Immunology 95(2): 214-218 (1998)
The immunocytochemical detection of bromodeoxyuridine (BrdU) incorpoRated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorpoRated BrdU with specific anti-BrdU monoclonal antibodies is an accuRate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorpoRated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic prolifeRative information (such as the S-phase transit Rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis. IIB5 reacts with bromodeoxyuridine (BrdU) also when incorporated into nuclear DNA.The antibody is known to cross-react with Iododeoxyuridine (IdU). Although we have no specific information concerning chlorodeoxyuridine (CldU), it is to be expected that also this antigen is recognized by IIB5.Detection of BrdU incorporated into the DNA needs certain retrieval methods that open up the nucleus and the DNA allow the antibody to reach the antigen. see ref. 1, 5.
Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The Cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. RCK103 is a Cytokeratin antibody recognizing (amongst others) Cytokeratin 5. This monoclonal antibody stains basal cells in combined and stRatified epithelial tissues. It recognizes the stem cell population, including the so-called amplifying cells in the prostate epithelium.
G4E4 recognizes an epitope within the 74-182 C-terminal sequence (11kD peptide fragment) of human serum Cellular Retinol Binding Protein 1 (CRBP 1), a single-chain glycoprotein belonging to the superfamily of hydrophobic molecule transporter proteins, which is responsible for transport of retinol (vitamin A1) from the liver to peripheral target tissues, like the eye, where it mediates the cellular uptake. CRBP 1 is synthesized by hepatic parenchymal cells where it becomes bound to its ligand retinol and is then released into the circulation, where it binds further to the protein transthyretin, to form a transporting complex, which is big enough not to be lost by filtration through the kidney glomeruli. It is detected in nearly all tissues with higher expression in adult ovary, pancreas, pituitary gland, adrenal gland, and fetal liver.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
G4E4
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Reddy B. et al. Biochem. Int. 21: 367-376 (1990)
References 2:
Reddy B. et al. Molec. Immunol. 29: 511-516 (1992)
References 3:
Reddy B. et al. Molec. Immunol. 30: 1355-1360 (1993)
UACA (Uveal Autoantigen with Coiled-coil domains and Ankyrin repeats) is a 1,416 amino acid nuclear membrane protein. It was originally identified as an autoantigen in patients with panuveitis, a characteristic of Vogt-Koyanagi-Harada disease, and in patients with Graves' disease. UACA was also later identified as Nucling, a mRNA differentially expressed in F9 embryonal carcinoma cells, and that is up-regulated during cardiac muscle differentiation. UACA appears to function as a pro-apoptotic protein that recruits the apaf-1- pro-caspase-9 complex for the induction of apoptosis to mediate the cell-death pathway.
Antibody Isotype:
IgG1,kappa
Monosan Range:
MONOSAN
Clone:
AE-5
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Yamada, K., et al. Biochem. Biophys. Res. Commun. 280: 1169-1176 (2001)
RAb-50 reacts with SAD-Vnukovo and Pitman-Moore strains of rabies virus. It shows conformation dependent specificity for the viral envelope glycoprotein. In immunofluorescence test, SAD-Vnukovo strain is recognized by the antibody, while in Western blot, both strains are recognized by the antibody. In ELISA only SAD-Vnukovo strain is recognized. RAb-50 can neutralize the CVS strain of rabies virus.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
RAB-50
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Macikova, I. et al, Acta virol. 36: 541-550 (1992)
References 2:
Sajjanar B, et al, Neuropeptides. 57: 59-64 (2016)
References 3:
Chen Li, et al, Acta Pharmacol Sin. 32(3): 329337 (2011)
SFL23.6 is directed against an erythroid cell surface antigen, which is not glycophorin A. It shows a well-defined reactivity with cells of the erythroid lineage at all stages of maturation in the peripheral blood, bone marrow, and fetal liver. Non-erythroid lineages are negative by flow cytometry. SFL23.6 is positive on erythroleukemias and can be used to distinguish bone marrow nucleated erythroid precursors from malignant cells in bone marrow specimens
PAb 122 binds to the C-terminus (aa370-378) of both wild type and mutated p53. When microinjected into nuclei, PAb 122 blocked re-entry into the S-phase of the cell cycle. Mutation and/or allelic loss of p53 is one of the causes of a variety of mesenchymal and epithelial tumors. p53 Localizes in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution.
WA-1 reacts with human and other mammalian p21, a tumor suppressor protein, belonging to the CDI family. The intracellular protein p21 is a 21 kDa protein, also known as wild-type p53-activated fragment 1 (WAF1). It is an inhibitor of cyclin-dependent kinases (Cdks) and of proliferating-cell nuclear antigen (PCNA). It is induced by wild type p53, but not by mutated p53, by mezerein (anti-leukemic compound) and by interferon-ß. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma and thyroid carcinoma).
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
WA-1
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Kovaric, J. et al, Int. J. Oncol. 9(suppl.), 835 (1996)
Monoclonal antibody EP-3 recognizes an antigen associated with the cytoplasm of human macrophages resident in the thymus and other lymphoid tissues. Monoclonal antibody EP-3 produces a strong cytoplasmic staining pattern of cortical thymic macrophages in formalin fixed, paraffin embedded tissues specimens. It may therefore be used as a marker of this cell type and of tumors derived from the macrophage.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
EP-3
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Fleming MG, et al, J Cutan Pathol. 17(2): 77-81 (1990)
LN-5 reacts with human macrophages and displays lymph node germinal center and mantle zone B cell reactivity. It reacts with interdigitating reticulum cells, with tingible body and sinus histiocytes. It further reacts with certain tumor cells and also with normal nonlymphoid tissue like chief cells of the stomach and spermatogonia. LN-5 is negative on Hodgkins disease and non-Hodgkins lymphomas.
58-15 Recognizes riboncleoproteins (RNP), found predominantly in nuclear ribonucleoprotein (nRNP) particles, one of the main components of nucleoli. It identifies cells active in the cell cycle and hence can be used to measure the mitotic activity of cell populations. Since the antibody can be used in paraffin embedded tissue sections, it can identify actively cycling cells within routinely fixed tissue specimens. 58-15 Can be considered a pan nRNP antibody. Pan nRNP antibodies provide detection for a range of RNP proteins.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
58-15
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Clevenger, C.V. et al. J Histochem Cytochem 32: 757-765 (1984)
References 2:
Clevenger, C.V. et al. Cytometry 6: 208-214 (1985)
References 3:
Maryam A and Nigel WF, J Virol. 75: 12070-12080, (2001)
IPO-M6 reacts with human leukemia cell line HL-60 and immuno-precipitates two proteins with MW of 48 and 52 kDa. IPO-M6 does not stain B cell lines Daudy, PHS, Namalwa, RPMI-1788 and T-cell lines CCRF-HSB2, Jurkat and Molt-4. IPO-M6 can be applied for staining of monocytes and up to 10 % of lymphocytes from peripheral blood of healthy donors. Blast cells of patients with AMMonL (M5 following FAB classification), AMMonL (M4) and hairy cells leukemia are IPO-M6 positive. The antigen, defined by IPO-M6, is particularly expressed on blood cells from patients with infectious mononucleosis and CLL. Histiocytes and macrophages are also positive. Malignant cells from patients with AML (M1 and M2), T-ALL, B ALL are IPO-M6 negative.
1108-1 Recognizes a 55-50 kDa polypeptide associated with the early antigen of Epstein-Barr virus (EBV). p55 Has beenshown to be a phosphoprotein and p55-50 has strong DNA-binding activity preferentially to single-stranded DNA. Epstein-Barr virus is the causitive agent of infectious mononucleosis and is associated with two human neoplasms, Burkitt's lymphoma and nasopharyngeal carcinoma. Several EBV-related antigens associated with early or late functions of the viral genome have been identified. The early antigen may be virally or chemically induced in EBV infected cells and is the first detectable marker of EBV infection in human cells.
The alpha interferons are involved in virus resistance in target cells for these viruses. They are known to block cell proliferation and to regulate MHC class I antigen expression. The IFN? family has over 20 genes and pseudogenes in two families (I and II), one with a mature length of 166aa and one of 172aa. Cells producing IFN? are lymphocytes, monocytes, macrophages and cell lines such as Namalwa and KGI. Bioassays for IFN? include cytopathic effect blocking, by viruses such as VSV, SFV and BMCV, on their target cells. A number of receptors for IFN? are now known and seem to be expressed on most cell types. N27 is specific for human IFN?2 and does not cross react with human IFN?1. N27 reacts with linear peptide 43aa-53aa, placing the epitope outside the immunodominant regions I and II.
Antibody Isotype:
IgG1,kappa
Monosan Range:
MONOSAN
Clone:
N27
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Kontsek, P. et al., Mol Immunol. 29: 863-870 (1992)
References 2:
Kontsek, P. et al., Immunol. Lett. 35: 281-284 (1993)
The alpha interferons are involved in virus resistance in target cells for these viruses. They are known to block cell proliferation and to regulate MHC class I antigen expression. The IFN? family has over 20 genes and pseudogenes in two families (I and II), one with a mature length of 166aa and one of 172aa. Cells producing IFN? are lymphocytes, monocytes, macrophages and cell lines such as Namalwa and KGI. Bioassays for IFN? include cytopathic effect blocking, by viruses such as VSV, SFV and BMCV, on their target cells. A number of receptors for IFN? are now known and seem to be expressed on most cell types. 2-48 Is specific for human interferon ? 1 and does not cross react with human interferon ? 2.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
Feb-48
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Kontsek, P. et al., Mol Immunol. 29: 863-870 (1992)
The alpha interferons are involved in virus resistance in target cells for these viruses. They are known to block cell proliferation and to regulate MHC class I antigen expression. The IFN? family has over 20 genes and pseudogenes in two families (I and II), one with a mature length of 166aa and one of 172aa. Cells producing IFN? are lymphocytes, monocytes, macrophages and cell lines such as Namalwa and KGI. Bioassays for IFN? include cytopathic effect blocking, by viruses such as VSV, SFV and BMCV, on their target cells. A number of receptors for IFN? are now known and seem to be expressed on most cell types. 2-52 Is specific for human IFN?1 and does not cross react with human IFN?2.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
Feb-52
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Kontsek, P. et al., Mol Immunol. 29: 863-870 (1992)
Insulin is a protein consisting of an ?-chain of 21 amino acids and a ?-chain of 30 amino acids and produced in the ?-cells of the pancreas. E2-E3 is a specific insulin antibody as tested by ELISA and on human pancreatic tissue.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
E2-E3
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
De la Tour, D, et al, Mol. Endoc. 15: 476-483 (2001)
References 2:
Rajagopal, J, et al, Science 299: 363 (2003)
References 3:
Morisset, J, et al, J. Histochem. Cytochem. 51: 1501-1513 (2003)
The alpha interferons are involved in virus resistance in target cells for these viruses. They are known to block cell proliferation and to regulate MHC class I antigen expression. The IFN? family has over 20 genes and pseudogenes in two families (I and II), one with a mature length of 166aa and one of 172aa. Cells producing IFN? are lymphocytes, monocytes, macrophages and cell lines such as Namalwa and KGI. Bioassays for IFN? include cytopathic effect blocking, by viruses such as VSV, SFV and BMCV, on their target cells. A number of receptors for IFN? are now known and seem to be expressed on most cell types. N39 is specific for human IFN?2 and does not cross react with human IFN?1. N39 is directed against immunodominant epitope site I (aa112-148).
Antibody Isotype:
IgG1,kappa
Monosan Range:
MONOSAN
Clone:
N39
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Kontsek, P. et al., Mol Immunol. 29: 863-870 (1992)
References 2:
Kontsek, P. et al., Immunol. Lett. 35: 281-284 (1993)
Insulin is a protein consisting of an ?-chain of 21 amino acids and a ?-chain of 30 amino acids and produced in the ?-cells of the pancreas. 2D11-H5 is a specific insulin antibody as tested by ELISA and on human pancreatic tissue.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
2D11-H5
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
De la Tour, D, et al, Mol. Endoc. 15: 476-483 (2001)
References 2:
Rajagopal, J, et al, Science 299: 363 (2003)
References 3:
Morisset, J, et al, J. Histochem. Cytochem. 51: 1501-1513 (2003)
F1G4 reacts with GnRH receptors in the anterior pituitary. GnRH stimulates the gonadotrophs of the anterior pituitary to secrete luteinising hormone (LH) as well as follicle-stimulating hormone (FSH). The receptor contains seven hydrophobic transmembrane domains connected by hydrophilic extracellular, and intracellular loops characteristic of G protein couple receptors. Some cancers like ovarian and breast cancers sometimes carry GnRH receptors.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
F1G4
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Karande, A.A., et al., Molec. Cell. Endocrinol. 114: 51-56 (1995)
EP-4 recognizes the HLA-B27 cell surface antigen on human cells. HLA-B27 has been found to be highly associated (60%) with ankylosing spondylitis (Bekhterev's disease, MarieStrümpell disease or "bamboo spine"). While rheumatoid factor tests will be negative, testing for the presence of HLA-B27 in a patient can confirm the diagnosis of ankylosing spondylitis. Also postgonococcal arthritis and acute anterior uveitis are associated with HLA-B27.
Antibody Isotype:
IgM,kappa
Monosan Range:
MONOSAN
Clone:
EP-4
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Hansen JA et al. Hematol Oncol Clin North Am, 4(3): 507-515 (1990)
References 2:
El-Shabrawi, Y. et al. Ophthalmology 113: 695-700 (2006).
TU-02 reacts with the N-terminal domain of alpha-tubulin. Tubulin isotypes have been identified with tissue specific expression. Immunocytochemical studies using several mAbs revealed remarkable heterogeneity of tubulin within various nervous tissues. TU-02 reacts with tubulin in fixed tissues only, not in unfixed or live tissues or cells. Interphase microtubules are also stained by TU-02 in fixed tissues.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
TU-02
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Dráber, P. et. al. Eur.J.Cell.Biol. 41: 82-88 (1986)
References 2:
Dráber, P. et. al. Histochemistry 87: 151-155 (1987)
References 3:
Dráber, P. et. al. J. Cell Science 92: 519-528 (1989)
References 4:
Smertenko et al. Eur. J. Cell. Biol. 72: 104-112 (1997)
LN-1 reacts with CDw75, a neuraminidase sensitive cell surface sialoglycan which is present on the cell membrane and cytoplasm of germininal center B-cells and derived lymphomas. CDw75 has a function in cell adhesion and is the ligand for CD22. LN-1 reacts with RBC precursors in bone marrow, ductal and ciliated epithelial cells of kidney, breast, prostate, pancreas, lung, and with gioblastomas and astrocytomas, and also Reed Sternberg cells in Hodgkins disease.
Antibody Isotype:
IgM,kappa
Monosan Range:
MONOSAN
Clone:
LN-1
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Epstein AL et. al. J of Immunology 133: 1028-1036 (1984)
MBS-12 specifically detects AFP. This protein is one of the major serum proteins in the early life of mammals and through to be fetal counterpart of albumin. AFP production is reactivated in the adult during liver regeneration and hepatocarcinogenesis, though in some individuals it persists into adulthood naturally. It is positive on all yolk sac tumors, on some other germ cell tumors and on hepatocellular carcinomas.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MBS-12
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Stefanova, I. et al, J. Immunol. Methods. 111: 67-73 (1988)
D11 reacts specifically with human monocytes and macrophages, in all sorts of tissues. The 125/135 kDa antigen is present on the cell membrane as well as within cytoplasmic structures including lysosomes, and is different from CD68. Among tumors, histiocytomas and histiocytic lymphomas are positive. In ALL, positive reaction with D11 indicates B-lineage derivation. AML are negative.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
D11
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Rudinskaya T.D. et al. Immunol Lett. 33(1): 1-7 (1992)
References 2:
Frenkel M.A. et al. Gematol Transfuziol. 40(4): 13-16 (1995)
References 3:
Tupitsyn N.N et al., Int J Cancer. 68(2): 160-163 (1996)
References 4:
Petrovichev N.N et al., Acta Cytol. 41(2): 357-363 (1997)
EBS-IF-007 is human specific and reacts with rod domain 2B, aa 311-335, of keratin 19 (40 kDa). Keratin 19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-keratin 19 reacts with a wide variety of corresponding epithelial malignancies.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
EBS-IF-007
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Böttger, V. et al., Eur. J. Biochem. 231: 475-485 (1995)
References 2:
Karsten et al., Eur.J.Cancer Clin. Oncol. 21(6), 733-740 (1985)
References 3:
Kasper et al., Histochemistry 89(4), 369-377 (1988)
1011 Is specific for the major vault protein, a 104-kDa highly conserved protein interacting with estrogen receptor. It is one of a series of four mAbs which recognize different epitopes of the protein. Major vault proteins have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for >70% of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
1011
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Abbondanza, C. et al, J. Cell Biol. 141, 1301-1310 (1998)
C-04 Reacts with keratin 18 (45kDa) present in a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
C-04
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bártek et al. J. of Pathol. 164: 215-24 (1991)
References 2:
Bártková et al. Neoplasma 38(4): 439-46 (1991)
References 3:
Lauerová et al. Hybridoma 7: 495-504 (1988)
References 4:
Taylor Papadimitriou et.al. J. Cell Sci. 94: 403-13 (1989)
References 5:
Kova?ík et al. J. Tumor Marker Oncol. 5: 219 (1990)
HE-182 reacts with human blood group H type 5 and type 6 as confirmed with Chembiomed synthetic oligosaccharide-BSA conjugate in ELISA. It is expressed on endothelial cells, epithelial cells and granulocytes. Increased expression of this antigen has been observed on some tumor tissues such as gastric carcinomas, urothelial carcinomas, and colon carcinomas
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
HE-182
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Rouher Ph et al. Blood transfusion and immuohaematology, Arnette France 30 (5): 353-720 (1987)
HE-193 recognizes human blood group A (monofucosyl and difucosyl A antigens with chain types 1, 2, 3, 4, 5, 6,) and Forssmann antigen, which is normally not found in humans, but can appear on malignancies. De novo or increased expression of these antigens have been observed on some tumor tissues such as gastric carcinomas, urothelial carcinomas, and colon carcinomas. HE-193 does not react with normal tissue sections of donors with blood group B and 0 but it reacts specifically with malignant tissues in these individuals. HE-193 is applicable for red cell agglutination, tissue staining and immunofluorescence tests
HEB-29 shows specific staining of erythrocytes and vascular epithelium of blood group B and AB controls and no staining in group A or O controls. However, tumors in A or O individuals may carry B antigen and stain with HEB-29. HEB-29 is applicable for red cell agglutination, tissue staining and immunofluorescence tests.
IPO-74 reacts with human leukemia cell line HL-60 and shows a band at MW 127 kDa (reduced) both in immunoprecipitation and Western blotting. In peripheral blood only subsets of B cells, T cells, neutrophilic and eosinophilic granulocytes are positive. All monocytes are positive. Cell lines U937 and K562 are positive, while Daudy, Raji, Jurkat and Molt-4 are negative, as are IL2-dependent T cell lines/clones.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
IPO-74
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Fleming MG, et al, J Cutan Pathol. 17(2): 77-81 (1990)
LN-6 reacts with vimentin, a 58kDa protein, and specifically with a non-hematopoietic epitope of vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Vimentin is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells, and endothelium. Antibody against vimentin is useful as part of an antibody panel for differential diagnosis of tumors of unknown origin. It does not react with leukocyte common antigen-positive tissues such as lymphomas and leukemias.
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
LN-6
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Stathopoulos, E, et al, J. Histochem. Cytochem. 37: 1363-1370 (1989)
E3 reacts with keratin 17 (45 kDa). The antibody reveals myoepithelial cells, basal cells and proliferating epithelia in some benign epithelial tumors as well as malignant carcinomas.
PLAP is a tissue specific, throphoblast-derived, 58 kDa, glycosyl-phosphatidylinositol (GPI)- anchored, dimeric, Zn2+ metallated glycoprotein, only found in humans, orangutans and chimpanzees, that catalyzes the hydrolysis of phosphomonoesters into an inorganic phosphate and an alcohol. It is present in the placenta and serum of pregnant women and in high frequency in gynecological and testicular cancers and in lower frequency in other tumors. The three tissue-specific APs in humans, PLAP, germ cell AP (GCAP) and intestinal AP, are 90-98% homologous. Non tissue specific AP is found in kidney, liver and bone. F5C2 binds equally well to all common allelic variants (S,F, FS and I) of PLAP and to some variants of AP from normal human testis, while antibody H7E8 reacts with all variants of AP in normal human testis.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
F5C2
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Millan J.L. et al, Eur. J. Biochem. 136: 1-7 (1983)
TV-1 recognizes fibronectin in frozen and paraffin sections of human, pig, mouse and rat tissues. Specifically, it stains this extracellular adhesive glycoprotein in connective tissues and blood vessels. TV-1 does not recognize the soluble dimeric form of fibronectin (plasma fibronectin) but strongly stains matrix fibronectin in tissues. Cellular fibronectin is widely distributed in the stroma of many malignant tumors
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
TV-1 (2755-8, EP-5)
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Epstein, AL. et al. Cancer Res. 55(12): 2673-80 (1995)
EP-4 recognizes the HLA-B27 cell surface antigen on human cells. HLA-B27 has been found to be highly associated (60%) with ankylosing spondylitis (Bekhterev's disease, MarieStrümpell disease or "bamboo spine"). While rheumatoid factor tests will be negative, testing for the presence of HLA-B27 in a patient can confirm the diagnosis of ankylosing spondylitis. Also postgonococcal arthritis and acute anterior uveitis are associated with HLA-B27.
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
EP-4
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Hansen JA et al. Hematol Oncol Clin North Am, 4(3): 507-515 (1990)
References 2:
El-Shabrawi, Y. et al. Ophthalmology 113: 695-700 (2006)
PLAP is a tissue specific, throphoblast-derived, 58 kDa, glycosyl-phosphatidylinositol (GPI)- anchored, dimeric, Zn2+ metallated glycoprotein, only found in humans, orangutans and chimpanzees, that catalyzes the hydrolysis of phosphomonoesters into an inorganic phosphate and an alcohol. It is present in the placenta and serum of pregnant women and in high frequency in gynecological and testicular cancers and in lower frequency in other tumors. The three tissue-specific APs in humans, PLAP, germ cell AP (GCAP) and intestinal AP, are 90-98% homologous. Non tissue specific AP is found in kidney, liver and bone. H7E8 binds equally well to all common allelic variants (S,F, FS and I) of PLAP as to AP from normal human testis, while antibody F5C2 reacts with some samples of normal human testis only.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
H7E8
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Millan J.L. et al, Eur. J. Biochem. 136: 1-7 (1983)
EBS-IF-004 reacts specifically with keratin 14 (50kDa) in immunoblotting. In tissue sections EBS-IF-004 is positive with basal cells of non-keratinizing stratified epithelia, basal cells and suprabasal cells of the epidermis and gingiva, myoepithelial cells and squamous cell carcinomas.
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
EBS-IF-004
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Ivanyi, D. et al. Am. J. Vet. Res. 53: 304-314 (1992)
References 2:
Ivanyi, D. et al. Cancer Res. 50(16): 5143-5152 (1990)
References 3:
Balm A.J.M. et al., Eur. Arch. Otorhinolaryngol. 253: 227-233 (1996)
The CD74 cluster, established during the IVth and Vth Leukocyte Typing Workshops, comprises four species of proteins (MW 41/35/33 kDa), all coded by a single gene, consisting of nine exons. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. In tissue sections anti-CD74 show a binding pattern very similar to that of anti-HLA-DR. It binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. EBS-CD-041 epitope is localized in the extracellular domain of CD74.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-041
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Epstein A.L. et al. J. Immunol. 133: 1028-1036 (1984)
References 2:
Marder, R.J. et al. Lab. Invest. 52: 497-504 (1985)
References 3:
Lazova R. et al. Cancer 79: 2115-2124 (1997).
References 4:
Pich, A. et al. Eur J Basic Appl Histochem. 35(1): 81-9 (1991)
EBS-I-014 reacts with flagellar core protein of Borrelia species including B.burgdorferi. B.burgdorferi is a species of bacteria of the spirochete class of the genus Borrelia causing Lyme disease, a vector-borne, multisystem inflammatory disease which is transmitted to humans by the bite of ticks of the Ixodes ricinus complex
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
EBS-I-014
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Busch, U. et al, J. Clin. Microbiol. 34: 1072-1078 (1996)
EBS-I-001 reacts with a soluble excreted antigen in ELISA. This determinant is unaffected by frozen storage of specimens, unlike antibodies to flagellar antigens which require freshly cultured organisms.
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
EBS-I-001
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Altekruse, SF, at al, Emerg Infect Dis. 5: 28-35 (1999)
FR4A5 reacts with CD15 (220 kDa). CD15 contains the pentasaccharide lacto-n-fucopentaose III and plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance. It is also expressed on a wide variety of other tumor cells including myeloid leukemia, breast, colorectal, and lung cancer cells. Cross reactivity has been observed with Glc?1-6glc?1-4Glc, Tn, blood group H1, and maltose.
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
FR4A5
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Manimala J.C. et al. Glycobiology. 17(8): 17C-23C (2007)
References 2:
Gildersleeve J. et al. Glycobiology. 18(0): 746 (2008)
EBS-I-002 reacts with a soluble excreted antigen in ELISA. This determinant is unaffected by frozen storage of specimens, unlike antibodies to flagellar antigens which require fresh cultured organism. Positive: C. jejuni, type 1 (K807, K858, K634) Type 2, C. coli, C. hyointestinalis, C lardis. Cross reacts with Staphylococcus aureus and weakly with Pseudomonas fluorescens. Negative: C. fetus, C. fetus intestinals, C. faecalis, H. pylori, Listeria monocytogenes, Actinomyces israelii, E. coli, Lactobacillus casei, Bacillus cereus, C. freundi, Salmonella Virchow, Streptococcus faecalis and Enterobacter aerogenes.
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
EBS-I-002
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Baily E.L. et al. Mol. Ecol 24(1): 208-21 (2015)
References 2:
Haddock G et al. microbiology 156(10): 3079-84 (2010)
References 3:
Altekruse, SF, at al, Emerg Infect Dis. 5: 28-35 (1999)
3-3A reacts with determinants of chain A type 3 and 4 and chain H type 3 and 4, but not with type 1 and 2 chain structures. It is not reactive with immunodominant A trisaccharide. Increased expression of this antigen has been observed on some tumor tissues such as gastric carcinomas, urothelial carcinomas, and colon carcinomas. 3-3A does not react with normal tissue sections of donors with blood group B and 0 but it reacts specifically with malignant tissues in these individuals.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
3-3A
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bara, J. et al. Biochem. J. 254: 185-193 (1988)
References 2:
Yasumsds, I. et al. Glycoconjugate J. 3: 187-202 (2000)
References 3:
Rouger et al. Blood transfusion and immunohematol. 30(5): 252-720 (1987)
568 Reacts with the 8th type III repeat in the cell-binding domain of human fibronectin (220 kDa monomer; 440 kDa, dimer). Fibronectins are present in basement membranes, intestinal connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
568
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Christensen, L. et al, APMIS 98(7): 615-623 (1990)
References 2:
Christensen, L. et al, APMIS suppl. 26: 1-39 (1992)
References 3:
Yong, JL. et al, Int J Clin Exp Pathol 3(2): 210-216 (2010).
EBS-O-166 specifically reacts with tenascin-C, an extracellular matrix glycoprotein of 210 kD. It recognizes those forms of tenascin that are produced by both normal and hyperproliferative (also neoplastic) tissues. Tenascin/hexabrachion/cytotactin is an extracellular matrix glycoprotein, widely expressed during embryogenesis. In adults, it is restricted to certain epithelial-stromal interfaces and increases markedly in hyperproliferative diseases and in stroma of many neoplasms, including gliomas, breast, squamous and lung carcinomas.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-O-166
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Verstraeten, AA et al., Br. J. Derm. 127(6), 571-574 (1992)
1032 Is specific for the major vault protein, a 104-kDa highly conserved protein interacting with estrogen receptor. It is one of a series of four mAbs which recognize different epitopes of the protein. Major vault proteins have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for >70% of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
1032
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Abbondanza, C. et al, J. Cell Biol. 141, 1301-1310 (1998)
Until recently, immunological markers for myeloid cells have been lacking, especially those which identify different levels of cellular differentiation. The BM series provides a new panel of monoclonal antibodies which stain early precursor and mature forms of human myeloid cells. This panel of monoclonal antibodies reacts with antigenic determinants present in normal myeloid cells and leukemias of similar derivation. BM-2 recognizes a cytoplasmic antigen expressed in mature human granulocytes (polys) residing in lymphoid and non-lymphoid tissues. It does not react with any other cell type in human tissues.
1E8-G6 reacts with TGF-alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. 1E8-G6 is completely blocked by the peptide used for immunization. TGFalpha (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. In sections both cytoplasma and cell membranes are stained
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
1E8-G6
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bebók, Zs, et al. Breast Cancer Res.Treatm. 29: 229-235 (1994)
EBS-I-100 reacts with C. difficile Toxin A, but not with V. cholerae subunit a, V. cholerae toxin, Pseudomonas aeruginosa exotoxin A, H-LT, P-LT. C. difficile is a major nosocomial pathogen that causes antibiotic-associated colitis and mediates inflammatory diarrhea by releasing two large protein enterotoxins (toxin A and toxin B) that are able to disrupt intestinal epithelial cells via their transferase activity and ability to monoglucosylate members of the Rho family. C. difficile toxin A is a toxin that is composed of 39 repeats that are responsible for binding to intestinal epithelial cell surface carbohydrates. C. difficile toxin A causes significant apoptosis of colonocytes which contributes to the formation of ulcers and pseudo-membranes in a pathway that involves p38-dependent activation of p53 and induction of p21, leading to cytochrome c release and caspase-3 activation through Bak activation.
Antibody Isotype:
IgG3-K
Monosan Range:
MONOSAN
Clone:
EBS-I-100
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Kim H, et al, Gastroenterology 129: 1875-1888 (2005)
References 2:
Carter JP, et al, Gut Microbes. 1(1): 5864 (2010)
Ten to forty percent of the patients with acquired immunodeficiency syndrome (AIDS) develop cytomegalovirus (CMV) infections. In some patients with AIDS, CMV is detected in the bronchoalveolar lavage fluid (BALF), urine, and other specimens, even when there are no symptoms of CMV disease. An indicator of active CMV infection is needed to facilitate the diagnosis of CMV disease in patients with AIDS or HIV infection. CMV p65 antigen was detected in the leukocytes of both the peripheral blood and BALF during the early phase of CMV disease.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
CMV221
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Hanabusa H, et al, Kansenshogaku Zasshi. 68(9): 1105-12 (1994)
Ten to forty percent of the patients with acquired immunodeficiency syndrome (AIDS) develop cytomegalovirus (CMV) infections. In some patients with AIDS, CMV is detected in the bronchoalveolar lavage fluid (BALF), urine, and other specimens, even when there are no symptoms of CMV disease. An indicator of active CMV infection is needed to facilitate the diagnosis of CMV disease in patients with AIDS or HIV infection. CMV p65 antigen was detected in the leukocytes of both the peripheral blood and BALF during the early phase of CMV disease
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
CMV-224
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Hanabusa H, et al, Kansenshogaku Zasshi. 68(9): 1105-12 (1994)
TU-02 reacts with the N-terminal domain of alpha-tubulin. Tubulin isotypes have been identified with tissue specific expression. Immunocytochemical studies using several mAbs revealed remarkable heterogeneity of tubulin within various nervous tissues. TU-02 reacts with tubulin in fixed tissues only, not in unfixed or live tissues or cells. Interphase microtubules are also stained by TU-02 in fixed tissues.
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
TU-02
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Dráber, P. et. al. Eur.J.Cell.Biol. 41: 82-88 (1986)
References 2:
Dráber, P. et. al. Histochemistry 87: 151-155 (1987)
References 3:
Dráber, P. et. al. J. Cell Science 92: 519-528 (1989)
References 4:
Smertenko et al. Eur. J. Cell. Biol. 72: 104-112 (1997)
108-2C5 recognizes an intralocus determinant present on a limited number of HLA-A locusencoded gene products (HLA-A2, -A3, A28, -A29, -A30, -A31 and -Aw33). Furthermore, by testing its reactivity with HLA-A2 natural variants and mutants, the importance of amino acid residues 79 and/or 80 of the alpha1 domain was demonstrated in the formation of an intralocus HLA-A determinant.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
108-2C5
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Lozano, F. et al., Immunogenetics 30: 50-53 (1989)
References 2:
Lozano, F. et al., Tissue Antigens 35: 193-195 (1990)
References 3:
Domenech, N. et al., Human Immunol 30: 140-146 (1991)
References 4:
Ryschich, E, et al, Clin Cancer Res. 11(2 Pt 1): 498-504 (2005)
UACA (Uveal Autoantigen with Coiled-coil domains and Ankyrin repeats) is a 1,416 amino acid nuclear membrane protein. It was originally identified as an autoantigen in patients with panuveitis, a characteristic of Vogt-Koyanagi-Harada disease, and in patients with Graves' disease. UACA was also later identified as Nucling, a mRNA differentially expressed in F9 embryonal carcinoma cells, and that is up-regulated during cardiac muscle differentiation. UACA appears to function as a pro-apoptotic protein that recruits the apaf-1- pro-caspase-9 complex for the induction of apoptosis to mediate the cell-death pathway.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
AE-5
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Yamada, K., et al. Biochem. Biophys. Res. Commun. 280: 1169-1176 (2001)
VEGF-21 reacts with Vascular Endothelial Growth Factor, also known as Vascular Permeability Factor (VEGF/ VPF) and is the key mediator of angiogenesis. The MWs are 19- 22kDa (reducing) and 38kDa-44kDa (non-reducing). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis. In addition to endothelial cells, VEGF and VEGF receptors are expressed on numerous non-endothelial cells including tumor cells.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
VEGF-21
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Tischer E. et al. J. Biol. Chem 266: 11947-11954 (1991)
EBS-huA recognizes the third constant domain (CH3) of the alpha chain of human IgA and reacts with both IgA1 and IgA2 isotypes and not with other types of immunoglobulins. IgA type antibodies are secreted by B-cells associated with mucosal epithelia and therefore indicate malignancy if found in lymphoid infiltrates at other locations. Detection of IgA antibodies to EBV in serum may indicate nasopharyngeal carcinoma. Rising IgA anti EBV levels are associated with progression of disease.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-huA
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Biewenga J. et al, Mol. Immunol. 23: 761-767 (1986)
References 2:
Biewenga J. et al, Adv. Exp. Med. Biol. 216B: 1239-1249 (1987)
References 3:
Mestecky J. et al, J. Immunol. Meth. 193: 103-148 (1996)
References 4:
Oortwijn B.D. et al, Mol Immunol. 44(5):966-73 (2007)
Ten to forty percent of the patients with acquired immunodeficiency syndrome (AIDS) develop cytomegalovirus (CMV) infections. In some patients with AIDS, CMV is detected in the bronchoalveolar lavage fluid (BALF), urine, and other specimens, even when there are no symptoms of CMV disease. An indicator of active CMV infection is needed to facilitate the diagnosis of CMV disease in patients with AIDS or HIV infection. CMV p65 antigen was detected in the leukocytes of both the peripheral blood and BALF during the early phase of CMV disease.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
CMV-227
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Hanabusa H, et al, Kansenshogaku Zasshi. 68(9): 1105-12 (1994)
616 Reacts with the N-terminal fibrin and heparin-binding domain. Specificity was established by Western blotting with purified 29 kDa domain from two different sources. Fibronectins, 220 kDa monomer; 440 kDa dimer, are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
616
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Grant, M B, et al. Diabetes, 47: 1311-7 (1998)
References 2:
Homandberg, G.A. et al. Osteoarthritis and Cartilage 6: 231244 (1998)
References 3:
Klueh, U, et al. Biomaterials 24(22) : 3877-84 (2003)
References 4:
Ljubimov, A.V. et al. Exp. Cell Res. 165: 53040 (1986)
References 5:
Mirmalek-Sani SH. et al. Biomaterials 165: 548895 (2013)
License is required for use outside research field. Fibrinogen is a protein produced by the liver which helps stop bleeding by helping blood clots to form. Fibrinogen gets deiminated (conversion from arginin to citrullin) by Peptidyl Arginine Deïminase (PAD) in inflamed joints in patients that develop rheumatoid arthritis. Citrulline, while being an amino acid, is not built into proteins during protein synthesis, as it is not coded for by DNA, yet several proteins are known to contain citrulline. Proteins that normally contain citrulline residues include myelin basic protein (MBP), filaggrin, and several histone proteins, while other proteins, like fibrin and vimentin can get deiminated during cell death and tissue inflammation. Patients with rheumatoid arthritis often (at least 80% of them) develop an immune response against proteins containing citrulline. Although the origin of this immune response is not known, detection of antibodies reactive with citrulline containing proteins or peptides is now becoming an important help in the diagnosis of rheumatoid arthritis.
Chicken IgY is specific to chickens and is the counterpart to IgG from mammals. Chickens transfer high quantities of IgY into the egg yolk and harvesting antibodies from eggs eliminates the need for the invasive bleeding procedure. One weeks eggs can contain 10 times more antibodies than the volume of rabbit blood obtained from one weekly bleeding. Due to the phylogenetic distance between birds and mammals, there is greater potential of producing a higher percentage of specific antibody against mammalian antigens when using chickens [1]. Since chicken IgY does not cross-react with mammalian IgG [2] and does not bind bacterial or mammalian Fc receptors [3], non-specific binding is reduced, and the need for cross-species immunoabsorptions is also eliminated [4].
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
08C
Concentration:
n/a
Storage buffer:
lyophilized in a 10 mM ammonium
Storage:
2-8°C
References 1:
Jensenius et al. J. Immunol Meth 1981; 46:63
References 2:
Ambrosius et al. Vet Immunol. Immunopathol;17:57
References 3:
Larsson et al, J. Immunol Meth 1988; 108:205
References 4:
Larsson et al. Comp. Immun.Microbiol Infe. Dis 1990; 13:199
License is required for use outside research field. Fibrinogen is a protein produced by the liver which helps stop bleeding by helping blood clots to form. Fibrinogen gets deiminated (conversion from arginin to citrullin) by Peptidyl Arginine Deïminase (PAD) in inflamed joints in patients that develop rheumatoid arthritis. Citrulline, while being an amino acid, is not built into proteins during protein synthesis, as it is not coded for by DNA, yet several proteins are known to contain citrulline. Proteins that normally contain citrulline residues include myelin basic protein (MBP), filaggrin, and several histone proteins, while other proteins, like fibrin and vimentin can get deiminated during cell death and tissue inflammation. Patients with rheumatoid arthritis often (at least 80% of them) develop an immune response against proteins containing citrulline. Although the origin of this immune response is not known, detection of antibodies reactive with citrulline containing proteins or peptides is now becoming an important help in the diagnosis of rheumatoid arthritis.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
23H2
Concentration:
n/a
Storage buffer:
lyophilized in a 10 mM ammonium bicarbonate buffer. Each vial contains 2 mg BSA
SC-05 reacts with a reduction resistant epitope on 80 kDa human secretory component (both free and bound to SIgA). Secretory component is differentially expressed in epithelium, thus SC-05 can identify subpopulations of epithelial cells and epithelial differentiation. Secretory component negative cell lines are not stained with SC-05.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
SC-05
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Kvale, D. et al, Int J Cancer 42(4): 638-641 (1988)
References 2:
Bartek, J. et al, Histochem 91(3): 235-244 (1989)
References 3:
Bartek, J. et al, Histochem J 22(10): 537-534 (1990)
License is required for use outside research field. Fibrinogen is a protein produced by the liver which helps stop bleeding by helping blood clots to form. Fibrinogen gets deiminated (conversion from arginin to citrullin) by Peptidyl Arginine Deïminase (PAD) in inflamed joints in patients that develop rheumatoid arthritis. Citrulline, while being an amino acid, is not built into proteins during protein synthesis, as it is not coded for by DNA, yet several proteins are known to contain citrulline. Proteins that normally contain citrulline residues include myelin basic protein (MBP), filaggrin, and several histone proteins, while other proteins, like fibrin and vimentin can get deiminated during cell death and tissue inflammation. Patients with rheumatoid arthritis often (at least 80% of them) develop an immune response against proteins containing citrulline. Although the origin of this immune response is not known, detection of antibodies reactive with citrulline containing proteins or peptides is now becoming an important help in the diagnosis of rheumatoid arthritis.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
20B2
Concentration:
n/a
Storage buffer:
lyophilized in a 10 mM ammonium bicarbonate buffer. Each vial contains 2 mg BSA
618 Recognizes an epitope contained within the gelatin binding domain of human fibronectin Fibronectins, 220 kDa monomer; 440 kDa dimer, are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
618
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Ljubimov, A.V. et al. Exp. Cell Res. 165,53040 (1986)
EBS-I-024 reacts with a conserved domain on EBNA1 (AA 430-442), present in all EBV isolates. EBNA1 is expressed in all viral latency stages in vitro and in vivo.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-I-024
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Chen, MR et al. J. Virol. 67(8): 4875-85 (1993)
References 2:
Chen, MR et al. J. Biomed. Sci. 5(3): 173-9 (1998)
References 3:
Sivachandra, N, et al, J. Virol. 86(1): 60-68 (2012)
License is required for use outside research field. Fibrinogen is a protein produced by the liver which helps stop bleeding by helping blood clots to form. Fibrinogen gets deiminated (conversion from arginin to citrullin) by Peptidyl Arginine Deïminase (PAD) in inflamed joints in patients that develop rheumatoid arthritis. Citrulline, while being an amino acid, is not built into proteins during protein synthesis, as it is not coded for by DNA, yet several proteins are known to contain citrulline. Proteins that normally contain citrulline residues include myelin basic protein (MBP), filaggrin, and several histone proteins, while other proteins, like fibrin and vimentin can get deiminated during cell death and tissue inflammation. Patients with rheumatoid arthritis often (at least 80% of them) develop an immune response against proteins containing citrulline. Although the origin of this immune response is not known, detection of antibodies reactive with citrulline containing proteins or peptides is now becoming an important help in the diagnosis of rheumatoid arthritis.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
3D1
Concentration:
n/a
Storage buffer:
lyophilized in a 10 mM ammonium bicarbonate buffer. Each vial contains 2 mg BSA
EBS-I-025 reacts with a conserved repeat domain on LMP1 (AA 268-286), present in all EBV isolates. LMP1 is expressed in most viral latency stages in vitro and in vivo
Protein tyrosine phosphatases (PTPs) are the enzymes that are instrumental in determining the spatial and temporal balance between the tyrosine phosphorylated and non-phosphorylated targets, and thus coordinately regulate these cellular responses to extracellular cues. The Ptprr gene gives rise to 4 different neuronal phosphatases which differ in length of their Nterminal part and subcellular localization. PTPBR7 (72 kDa) is receptor-like, PTP-SL (60 kDa) is membrane associated and PTPPBS? (42 and 37 kDa) is a cytosolic phoaphatase. This antibody is directed to the common part of these proteins.
Antibody Isotype:
IgG2
Monosan Range:
MONOSAN
Clone:
6A6
Concentration:
n/a
Storage buffer:
lyophilized in a 10 mM ammonium bicarbonate buffer. Each vial contains 2 mg BSA
EBS-huKappa reacts with kappa light chain In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of kappa to lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-huKappa
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Takahashi, H. et al. Pathol Res Prac 189, 300-311 (1993)
References 2:
Momose, H., et al. Hum. Pathol 23: 1115-1119 (1992)
MUC4 (mucin 4) is a large membrane-anchored glycoprotein of the mucin family that is expressed by epithelial cells in various normal tissues including lung, bronchus, stomach, colon, and cervix. MUC4 is generally not detected in normal pancreas, but is expressed in the vast majority of pancreatic neoplasms, such as pancreatic ductal adenocarcinoma. Additionally, expression in various carcinomas has been reported, including gastric adenocarcinoma, colon adenocarcinoma, and lung adenocarcinoma.
Fluorescent proteins, like EBFP, can be used as protein "tags" to study the subcellular localization of proteins and/or their translocation upon stimulation and/or as markers for transfections in transient and stable expression systems. In immunoblot it cross reacts with GFP and YFP.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
7F10
Concentration:
n/a
Storage buffer:
lyophilized in a 10 mM ammonium bicarbonate buffer. Each vial contains 2 mg BSA
Fluorescent proteins, like EBFP, can be used as protein "tags" to study the subcellular localization of proteins and/or their translocation upon stimulation and/or as markers for transfections in transient and stable expression systems. In immunoblot it cross reacts with GFP and YFP.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
3A6
Concentration:
n/a
Storage buffer:
lyophilized in a 10 mM ammonium bicarbonate buffer. Each vial contains 2 mg BSA
EBS-huLambda reacts with human immunoglobulin lambda light chain (22,5 kDa). EBShuLambda does not react with T cells, monocytes, granulocytes or erythrocytes.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-huLambda
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Baryshnikov A. et al. Gemat.Transf. (Russian) N8, 4-7 (1990)
References 2:
Martinova T.et al., In: Problems in med biotech. and immunol. Infect.diseases. 11, 182-186, (1996)
EBS-IF-001 Does not react with keratin polypeptides in immunoblotting. The specificity for keratin was established on the basis of antibody reactivity with intracytoplasmic intermediate filaments in epithelial, vimentin negative, human and feline cells. Distribution of the epitope strongly suggests that EBS-IF-001 reacts with keratins 5 and 14, most probably with a heterotypic complex formed by these keratins. EBS-IF-001 reacts positively with basal cells of all stratified epithelia, with myoepithelial cells and with most squamous cell carcinomas. It is useful in immunohistochemistry for typing of basal cells.
The exosome, present in both the nucleus and cytoplasm of all eukaryotic cells, is a complex of 3'-5' exoribonucleases containing at least nine core components. Recently, it has been demonstrated, mainly by analyses in yeast, that the nuclear exosome is essential for rRNA processing and sn(o)RNA biogenesis. Furthermore, it is involved in the degradation of improperly processed mRNAs. The cytoplasmic exosome participates in normal mRNA turnover and in the degradation of inherently instable mRNAs that contain AU-rich elements. Therefore, the exosome plays a key role in RNA metabolism.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
15B3
Concentration:
n/a
Storage buffer:
lyophilized in a 10 mM ammonium bicarbonate buffer. Each vial contains 2 mg BSA
The nuclei of eukaryotic cells contain several classes of small RNA-protein complexes. SnRNPs (small nuclear ribonucleoproteins) are involved in splicing of pre-mRNAs (the excision of introns) in the nucleoplasm. RPP20 is part of such a complex. SnoRNPs (small nucleolar ribonucleoproteins) are involved in the maturation of pre-ribosomal RNA in the nucleoli. Pre-rRNA processing and modification require both the snoRNA and the protein constituents (such as Rpp20) of these complexes. Generally, snoRNPs contain a number of common proteins, which are shared with other snoRNPs of the same family, next to several particle-specific proteins. On average, snoRNPs contain about 6-10 protein subunits
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
1F11
Concentration:
n/a
Storage buffer:
lyophilized in a 10 mM ammonium bicarbonate buffer. Each vial contains 2 mg BSA
EBS-O-109 reacts with human ?-2 microglobulin, a 22 kDa protein, which associates noncovalently with the 44kDa ?1-chain of the HLA Class I complex found on all nucleated cells and on platelets. There is no reaction with erythrocytes, neither with non-human primate cells. The detection of ?-2 microglobulin in body fluids has been used as a tumor marker, renal failure marker and for monitoring patients with HIV infection.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
EBS-O-109
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Brodsky FM et al, lmmunol Rev 47: 3-61 (1979)
References 2:
Ryschich, E, et al, Clin Cancer Res. 11(2 Pt 1): 498-504 (2005)
Betts RL, et al. Monoclonal hybridoma antibodies: Techniques and applications. Edited by D. Hurrel. Uniscience series program. C.R.C. Press, Cleveland, OH: (1983), pp. 193-222
Giardiasisis a diarrheal illness caused by a single celled microscopic protozoan parasite, Giardia lamblia, also known as Giardia intestinalis. Giardia lamblia exists in two forms, an active form called a trophozoite, and an inactive form called a cyst. The active trophozoite attaches to the lining of the small intestine and is responsible for causing the signs and symptoms of giardiasis. The trophozoite cannot live long outside of the body and spread of infection is via the cyst, which is excreted in the host's feces. When it is ingested, stomach acid activates the cyst, and the cyst develops into the disease causing trophozoite in the new host. Giardiasis is diagnosed by finding cysts or trophozoites in the feces.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-I-039
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Misra, V, et al, Indian J. Pathol. Microbiol. 49: 519-523 (2006)
Membrane fusion is mediated by envelope glycoproteins for enveloped viruses like herpes simplex. Four of at least 10 viral glycoproteins are necessary and sufficient to facilitate fusion of herpes simplex to target cells. These four glycoproteins include glycoprotein B (gB), glycoprotein D (gD), glycoprotein H (gH) and glycoprotein L (gL). Fusion is dependent upon the expression of a gD receptor on target cell membranes.postive: HSV-1, Negative HSV-2.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
EBS-I-042
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bystricka, M, et al, Acta Virol. 43: 399-402 (1999)
Monoclonal antibody HPV-13E2 reacts with HPV-16 E6 peptide (MHQKRTAMFQDPPQERPRKLPQLC). Infection with specific types of HPV has been associated with an increased risk of developing cervical neoplasia. HPV types 6 and 11 have been associated with relatively benign diseases such as genital warts but types 16 and 18 are strongly associated with cervical, vaginal, and vulvar malignancies
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
HPV-13E2
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Beldermann, F. Virusprotein E6 des humanen Papillomavirus HPV-16, Thesis, University of Heidelberg, Germany (1995).
Monoclonal antibody HPV-4B12 reacts with HPV-16 E6 peptide (MHQKRTAMFQDPPQERPRKLPQLC). Infection with specific types of HPV has been associated with an increased risk of developing cervical neoplasia. HPV types 6 and 11 have been associated with relatively benign diseases such as genital warts but types 16 and 18 are strongly associated with cervical, vaginal, and vulvar malignancies.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
HPV-4B12
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Beldermann, F. Virusprotein E6 des humanen Papillomavirus HPV-16, Thesis, University of Heidelberg, Germany (1995).
Monoclonal antibody HPV-4G3 reacts with HPV-18 E6 peptide. Infection with specific types of HPV has been associated with an increased risk of developing cervical neoplasia. HPV types 6 and 11 have been associated with relatively benign diseases such as genital warts but types 16 and 18 are strongly associated with cervical, vaginal, and vulvar malignancies.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
HPV-4G3
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Beldermann, F. Virusprotein E6 des humanen Papillomavirus HPV-16, Thesis, University of Heidelberg, Germany (1995).
EBS-T-001 reacts with BCL10. Having an N-terminal caspase recruitment domain (CARD), BCL10 can induce apoptosis and activate NF-?B. It is found on subpopulations of normal B and T cells, and is associated with MALT1, a paracaspase that, like BCL10, can be found translocated in MALT lymphoma. In such cases either BCL10 or MALT1 or both are highly expressed, depending on the site of translocation. MALT lymphomas lacking this translocation exhibit much lower levels of expression. BCL10 has been shown to be functionally conserved all the way back to zebrafish
BLA36 is a developmentally regulated 36 kDa antigen expressed on the plasma membrane of B lymphocytes, Reed-Sternberg, and mononuclear Hodgkins cells. It also gives strong staining of B cell lymphomas including follicular center cell lymphomas (large and small cell types), mantle zone lymphomas, and immunoblastic lymphomas.
EBS-IF-003 reacts with 53kDa (CK13) and 56.6kDa (CK10) cytokeratin proteins as indicated by immunoblotting. On formalin-fixed, paraffin-embedded tissue sections EBS-IF-003 recognizes only CK13. On cryostat sections EBS-IF-003 serves as differentiation-related marker of all stratified epithelia; it stains all suprabasal cells in both cornifying and noncornifying stratified epithelia and more differentiated cells of squamous carcinomas. In paraffin sections EBS-IF-003 does not stain CK10 positive, CK13 negative epithelia, as for example epidermis.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
EBS-IF-003
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Ivanyi D. et al, J. Pathol. 159: 7-12 (1989)
References 2:
Ivanyi D. et al, Am. J. Vet. Res. 53: 304-314 (1992)
References 3:
Ivanyi D. et al, Am. J. Vet. Res. 54: 1095-1102 (1993)
References 4:
Kozaki, M et al, J. Vet. Med. Sci. 63(1): 1-4 (2001)
Bra23/9 reacts with a monomorphic determinant of human major histocompatibility (MHC) class I antigens (HLA-A, B and C). Human MHC class I antigens are expressed constitutively on all nucleated cells and platelets and are absent on erythrocytes. MHC class I antigens play a role in class I MHCassociated antigen presentation, inhibition of NK cell cytotoxicity, tumor surveillance, and tissue allotransplantation.
1014 Is specific for the major vault protein, a 104-kDa highly conserved protein interacting with estrogen receptor. It is one of a series of four mAbs which recognize different epitopes of the protein. Major vault proteins have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for >70% of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
1014
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Abbondanza, C. et al, J. Cell Biol. 141, 1301-1310 (1998)
MHC class II molecules are encoded by polymorphic MHC genes and consist of a non-covalent complex of an ? and ? chain. Helper T lymphocytes bind antigenic peptides presented by MHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes.
Antibody Isotype:
IgG2b-K
Monosan Range:
MONOSAN
Clone:
BraFB6
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Babusikova O et al., Neoplasma 32(6): 657-62 (1985)
A-492 reacts with adiponectin, an adipocytokin; adipocytokines are hormones produced in adipose tissue. Adiponectin is abundantly present in plasma and has an insulin like effect on glucose levels in the blood. Plasma adiponectin levels are found in insulin resistant patients who are obese, have diabetes mellitus type 2 or HIV-lipodystrophy. In women adiponectin levels tend to be higher than in men, which may be due to androgens suppressing adiponectin levels. Furthermore adiponectin and leptin are both indicated in regulating body weight through direct action on the hypothalamus, influencing appetite. Obese people have low adiponectin levels while levels in anorexia patients are high. Adiponectin acts as ligand for various receptors, two of which have been identified, one probably involved in carbohydrate assimilation, the other in tuning the rate of metabolism.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
A-492
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Dos Santos, E. et al, Oncol. Rep. 20: 971-977 (2008)
A-493 react with adiponectin, an adipocytokine. Adipocytokines are hormones produced in adipose tissue. Adiponectin is abundantly present in plasma and has insulin like effect on glucose levels in the blood. Plasma adiponectin levels are low in insulin resistant patients who are obese, have diabetes mellitus type 2 or HIV-lipodystrophy. In women adiponectin levels tend to be higher than men, which may be due to androgens suppressing adiponectin levels. Furthermore adiponectin and leptin are both indicated in regulating body weight through direct action on the hypothalamus, influencing appetite. Obese people have low adiponectin levels while levels in anorexia patients are high. Adiponectin acts as ligand for various receptors, two of which have been identified, one probably involved in carbohydrate assimilation, the other in tuning the rate of metabolism.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
A-493
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Dos Santos, E. et al, Oncol. Rep. 20: 971-977 (2008)
The alpha interferons are involved in virus resistance in target cells for these viruses. They are known to block cell proliferation and to regulate MHC class I antigen expression. The IFN? family has over 20 genes and pseudogenes in two families (I and II), one with a mature length of 166aa and one of 172aa. Cells producing IFN? are lymphocytes, monocytes, macrophages and cell lines such as Namalwa and KGI. Bioassays for IFN? include cytopathic effect blocking, by viruses such as VSV, SFV and BMCV, on their target cells. A number of receptors for IFN? are now known and seem to be expressed on most cell types. 2-52 Is specific for human IFN?1 and does not cross react with human IFN?2.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
Feb-52
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Kontsek, P. et al., Mol Immunol. 29: 863-870 (1992)
C-51 reacts with keratin 8 (52.5 kDa) + 18 (45 kDa) polypeptides and recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
C-51
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bártek et al. J. Pathol. 164: 215-24 (1991)
References 2:
Bártková et al. Neoplasma 38(4): 439-46 (1991)
References 3:
Wendl, J. et al. Anat. Histol Embryol. 164: 215-24 (1991)
References 4:
Thangappan, R et al. Cell. Polif. 42: 770-779 (2009)
MHC class II molecules are encoded by polymorphic MHC genes and consist of a non-covalent complex of an ? and ? chain. Helper T lymphocytes bind antigenic peptides presented by MHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes.
Antibody Isotype:
IgG3-K
Monosan Range:
MONOSAN
Clone:
Bra14
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Chorvath B et al. Neoplasma 34(4):417-425 (1987)
References 2:
Horejsi V et al. Tissue Antigens 32(1):6-11 (1988)
EBS-O-104 reacts with a monomorphic determinant of human major histocompatibility (MHC) class I antigens (HLA-A, B and C). Human MHC class I antigens are expressed constitutively on all nucleated cells lymphocytes such as lymphocytes, thymocytes, granulocytes, and bone marrow cells and also platelets but are absent on erythrocytes. MHC class I antigens play a role in class I MHCassociated antigen presentation, inhibition of NK cell cytotoxicity, tumor surveillance, and tissue allotransplantation.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
EBS-O-104
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Young NT et al. Hum Immunol 52(1): 1-11 (1997)
References 2:
Krensky AM et al, Transplant Proc 28(6): 3026-8 (1996)
References 3:
Hansen JA et al, Hematol Oncol Clin North Am 4(3): 507-515 (1990)
EBS-O-114 reacts with HLA Class II. The antibody was shown to strongly block cytotoxic activity of T4+ cytotoxic T cell clones. Distribution studies on cell lines suggested that EBS-O-114 is directed against a DQ rather than a DR antigen.
MHC class II molecules are encoded by polymorphic MHC genes and consist of a non-covalent complex of an ? and ? chain. Helper T lymphocytes bind antigenic peptides presented by MHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes.
Antibody Isotype:
IgG2b-K
Monosan Range:
MONOSAN
Clone:
LN-3
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Marder, R.L. et al. Lab. Invest. 52: 497-504 (1985)
References 2:
Andrade, R.E. et al. Human Pathology 19: 932-941 (1988)
References 3:
Azumi N. et al. Human Pathology 19: 1376-1382 (1988)
MoBU-1 is reactive with cells containing incorporated 5-bromodeoxyuridine (BrdU) showing a clear, nucleus confined, speckled pattern. BrdU is an analogue of thymidine and can be introduced to proliferating cells in vitro or in vivo, which in turn incorporate BrdU into the DNA during S phase, prior to cell division. Immunocytochemical staining with MoBU-1 will show the degree of proliferation, detecting nucleated cells which have incorporated BrdU in place of thymidine into their DNA.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
MoBU-1 (85-2C8)
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Harms et al. Acta Histochemica, Suppl. Band 36, 353-359 (1988)
EBP-333 reacts with C/EBP? or CCAAAT/enhancer-binding protein beta, a transcription factor which is not only critical for normal macrophage functioning and differentiation, but affects a variety of other factors as well, such as cytokines (IL-6; IL-4; IL-5 and TNF-alpha), neurotransmitters and other neuronal factors and processes like muscle repair and the development of multi drug resistance in tumors (P-glycoprotein). Observations have implied that manipulation of the transcription factors involved may make it possible to modulate multidrug resistance, while leaving normal function of P-glycoprotein intact.
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
EBP-333
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Couturier, C et al, Arterioscler Thromb Vasc Biol. 20(12): 2559-65 (2000)
121SLE reacts with CA19-9 (>400 kDa) or sialyl Lea structure, which is synthesized from type 1 blood group precursor chains and is present in individuals expressing the Lea and/or Leb blood group antigens. 121SLE also binds to some extend to the afuco version of SLe a (LSTa; CA50). In normal tissues, CA19-9 is present in ductal epithelium of the breast, kidney, salivary, gland and sweat glands. Its expression is greatly enhanced in serum as well as in the majority of tumor cells in gastrointestinal (GI) carcinomas, including adenocarcinomas of the stomach, intestine and pancreas. 121SLE was typed in the ISOBM TD-6 workshop.
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
121SLE
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Rye PD. et al, Tumor Biol 19 (5): 390-420, (1998).
References 2:
Christopher MG. et al, Cytojournal 8: 7 (2011)
References 3:
Rouger et al. Blood transfusion and immunohematol. 30(5): 353-720 (1987)
4D11 reacts with CRASH, a 308 AA glycoprotein, sharing 32% homology with human asparaginase and identical to Asparaginase-Like Protein (ALP) found in rat sperm. CRASH is found in a variety of tumors but only occasionally in normal tissues. Especially ovarian cancers are highly positive. CRASH is further found in brain tumors, some colonic cancers, some lung cancers, some prostate cancers, uterine cancers, some breast cancers and some thyroid cancers. It was not found in serum. In Western blot 3 bands are demonstrated of 45 kDa, 28 kDa and 15 kDa.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
4D11
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Weidle, UH et al, Anticancer Res. 29(4): 951-63 (2009).
7B6 reacts with CRASH, a 308 AA glycoprotein sharing 32% homology with human asparaginase and identical to Asparaginase-Like Protein (ALP) found in rat sperm. CRASH is found in a variety of tumors but only occasionally in normal tissues. Especially ovarian cancers are highly positive. CRASH is further found in brain tumors, some colonic cancers, some lung cancers, some prostate cancers, uterine cancers, some breast cancers and some thyroid cancers. It was not found in serum. In Western blot 3 bands are demonstrated of 45 kDa, 28 kDa and 15 kDa.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
7B6
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Weidle, UH et al, Anticancer Res. 29(4): 951-63 (2009).
IPO-38 reacts with a 12-14 kDa protein, as found in Western blots of Raji cells, and appears in the mitotic cycle earlier than Ki-67. Lymphocytes, induced to early G1 phase by 12h exposure to PHA, will become positive while non-stimulated lymphocytes remain negative. Mononuclear cells and granulocytes of healthy donors are negative, while various forms of leukemia and lymphoma including Hodgkins disease are positive for IPO-38, as are many solid tumors such as some breast, gastric and colonic cancers for which it may serve as tumor progression marker.
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
IPO-38
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Sidorenko, S.P. et al., Gematol. Transfuziol. 35: 19-22 (1990)
References 2:
Sidorenko, S.P. et al., Experem. Oncol. 16: 145-150 (1994)
References 3:
Thosaporn W., et al., Oral Dis. 10(1): 22-6 (2004)
References 4:
Hao Y. et al.,J Proteome Res. Sep;7(9): 3668-77 (2008)
References 5:
Makohon N.V. et al., Fiziol Zh. 54(6): 49-57 (2008)
MFG-06 reacts with a glycoprotein of 40-45 kDa, known as Milk fat globule-EGF factor 8 protein (MFG-E8), lactadherin, p47 or milk fat globule 1 antigen. It is present on normal epithelial cells in various organs and considered a differentiation marker in carcinomas. It contains one EGF-like domain and 2 F5/8 type C domains. Functioning as a specific ligand for Integrin ?5 and Integrin ?3, MFG-E8 is thought to be involved in gamete interactions and cell attachment, possibly playing a role in fertilization and apoptosis. Additionally, MFG-E8 binds to rotavirus and inhibits its replication, thereby protecting the cell from viral infection. Overexpression of MFG-E8 is associated with breast cancer, suggesting that MFG-E8 may be related to tumorigenesis or progression. Among testicular carcinomas, seminomas are negative.
EBS-C-003 recognizes Ku protein or XRCC5/6 (X-ray repair cross complementing protein 5/6), involved in Pol II-directed transcription by virtue of its DNA binding activity, serving as the regulatory component of the DNA-associated protein kinase that phosphorylates Pol II and transcription factor Sp. Ku proteins also activate transcription from the U1 small nuclear RNA and the human transferrin receptor gene promoters. It serves as autoantigen in patients with rheumatic diseases.
EBS-O-148 can be used in ELISA, frozen sections, FACS and Western blot, however no method is known to date to retrieve the antigen in paraffin sections. Anti-hemoglobin antibodies can be used in diagnosis of anemias and as tumor marker in stool.
EBS-T-005 reacts with MADER or Melanoma-Associated Delayed Early Response protein, encoded by the NAB2 gene, belonging to the family of NGFI-A binding proteins. They modulate transcription induced by some members of the EGR (early growth response) family of transactivators. NAB proteins can form homo- or hetero-multimers with other EGR or NAB proteins through a conserved N-terminal domain, and repress transcription through two partially redundant C-terminal domains.
EBS-T-006 reacts with MADER or Melanoma-Associated Delayed Early Response protein, encoded by the NAB2 gene, belonging to the family of NGFI-A binding proteins. They modulate transcription induced by some members of the EGR (early growth response) family of transactivators. NAB proteins can form homo- or hetero-multimers with other EGR or NAB proteins through a conserved N-terminal domain, and repress transcription through two partially redundant C-terminal domains.
EBS-T-007 reacts with paracaspase MALT1, crucial for B- and T-cell activation/proliferation and activation of transcription factor NF-?B. It has an N-terminal death domain, two central immunoglobulin-like domains involved in the binding to the B-cell lymphoma 10 (BCL10) protein and a caspase-like domain. MALT1 and BCL10, can both be found translocated in MALT lymphoma. In such cases either BCL10 or MALT1 or both are highly expressed, depending on the site of translocation. Normal cells and MALT lymphomas lacking translocations exhibit much lower levels of expression.
47-8D3 reacts with macrophages and detects the well-known leukocyte L1, cystic fibrosis antigen. Detecting a single protein band of 14 kDa in Western blots of lysates of human monocytes and granulocytes, the antigen was identified as the calcium-binding protein MRP14, which is a member of the S100 family involved a.o. in regulating the cell cycle. MRP14 is also implicated in the abnormal differentiation of myeloid cells in the stroma of cancer. It is further found on squamous mucosal epithelia. When associated with MRP8 it forms the heterodimer calprotectin.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
47-8D3
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Flavell DJ. et al., J. Histochem. Cytochem. 35: 1217-1226 (1987)
References 2:
Facchetti F. et al., Am. J. Clin. Pathol. 92: 42-50 (1989)
References 3:
Bardadin KA. et al., J. Pathol. 164: 253-259 (1991)
References 4:
Goebeler M. et al., J. Leukocyte Biol. 55: 259-261 (1994)
POH-1 detects the three bands within the 34kDa region corresponding to the p34 protein cyclin dependent kinase 1 (cdk1) and its cleavage products. It is positive in immunoblotting on HeLa cell lysate and negative on fibroblast (LEP strain) cell lysate. A cdc2 homolog, the cdk2 protein kinase, does not react with POH-1. Activated cdk1 / p34cdc2 performs specific functions during mitosis, including nuclear envelope breakdown and chromosome condensation.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
POH-1
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Luká, J, et al. Eur. J. Biochem., 207: 169-176 (1992)
Bp53-12 reacts with an N-terminal epitope (aa 16-25) of both wild-type and mutated p53. This epitope is revealed in tissue sections only after formalin fixation. Mutation and/or allelic loss of p53 is one of the causes of a variety of mesenchymal and epithelial tumors. p53 Localizes in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution.
Antibody Isotype:
IgG2a-A
Monosan Range:
MONOSAN
Clone:
Bp53-12
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bártek J. et al, J Pathol. 169(1):27-34 (1993)
References 2:
Vogelstein and Kinzler, Cell 70: 523-526, (1992)
References 3:
Hollstein et al, Science 253: 49-53: (1991)
References 4:
Lane, D.P, Nature 358: 15-16: (1992)
References 5:
Donehower et al, Biochemic. Biophys. Acta 1155: 181-182, (1993)
EBS-T-059 recognizes an oncofetal antigen of 220kDa, identified as a tumor-associated glycoprotein (TAG72) with properties of a mucin and usually expressed by adenocarcinomas, but not by mesotheliomas and thus helps in the differential diagnosis of malignancies of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma. TAG72 is further used as serum marker for gastric cancer.
MAPKs are involved in directing cellular responses to a diverse array of potentially harmful stimuli, such as mitogens, osmotic stress, heat shock, proinflammatory cytokines, but also growth factors (mammals). Possibly located exclusively in the cell nucleus, they regulate cell functions including proliferation, gene expression, differentiation, mitosis, cell survival, and apoptosis. In order to become active, they require usually multiple phosphorylation events in their activation loops, including phosphorylation by MAP2 kinases (Ste-7 kinases), which in turn are phosphorylated by the MAP3 kinase family, of which many are located at the cell membrane. Thus through this pathway, stimuli can effectively be conveyed from the cell membrane to the nucleus. Inactivation of MAPKs takes place by several phosphorylases, including dedicated phophorylases.
MHC class II molecules are encoded by polymorphic MHC genes and consist of a non-covalent complex of an ? and ? chain. Helper T lymphocytes bind antigenic peptides presented by MHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes.
Antibody Isotype:
IgG2-K
Monosan Range:
MONOSAN
Clone:
EBS-O-110
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Sparrow RL, et al., Transplantation 42: 647-652 (1986)
References 2:
Chorvath B et al. Neoplasma 34(4): 417-425 (1987)
References 3:
Horejsi V et al. Tissue Antigens 32(1): 6-11 (1988)
1027 Is specific for the major vault protein, a 104-kDa highly conserved protein interacting with estrogen receptor. It is one of a series of four mAbs which recognize different epitopes of the protein. Major vault proteins have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for >70% of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
1027
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Abbondanza, C. et al, J. Cell Biol. 141, 1301-1310 (1998)
MHC class II molecules are encoded by polymorphic MHC genes and consist of a non-covalent complex of an ? and ? chain. Helper T lymphocytes bind antigenic peptides presented by MHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
Bra30
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Chorvath B et al. Neoplasma 34(4): 417-425 (1987)
References 2:
Horejsi V et al. Tissue Antigens 32(1): 6-11 (1988)
CDw78 (also called Ba antigen, Leu21 or LO panB a) is present on some immature and some mature B-cells. The antigen appears on B-cell progenitors preceding CD10, CD19, CD22, and CD37. It is expressed on resting B-cells and reappears and persists in the cytoplasm and on the cell surface until cytoplasmic Ig appears. Its expression is greatly increased after B-cell activation in vitro. It is also found on tissue macrophages and on epithelial cells, but not on T-cells, NK cells, monocytes, granulocytes, thymocytes or bone marrow stromal fibroblasts nor myeloid tissues. 60-3G2 was typed at CD workshop IV.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
60-3G2
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Pinchouk VG. et al, Anticancer Research 8: 1377-1380 (1988)
References 2:
Gluzman DF. et al, Tissue Antigens 33: 151 (1989)
References 3:
Sidorenko SP. et al, Neoplasma 39: 3-9 (1992)
References 4:
Moldenhauer et al, Leucocyte Typing IV, pp 155 162, (1989)
References 5:
Pezzuto et al, Leucocyte Typing IV, pp 165 174, (1989).
C11 reacts with keratins : 4,5,6,8,10,13 and 18. This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
C-11
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Vojt?ek, B. et al. Folia Biol., 35(6): 373-382, (1989)
References 2:
Vojt?ek, B. et al. Neoplasma 37(3): 333-342 (1990)
References 3:
Kova?ík J. et al. Int. J. Cancer, Suppl. 3: 50-55, (1988)
References 4:
Kova?ík J. et al. J. Tumor Marker Oncol. 5: 219 (1990)
The alpha interferons are involved in virus resistance in target cells for these viruses. They are known to block cell proliferation and to regulate MHC class I antigen expression. The IFN? family has over 20 genes and pseudogenes in two families (I and II), one with a mature length of 166aa and one of 172aa. Cells producing IFN? are lymphocytes, monocytes, macrophages and cell lines such as Namalwa and KGI. Bioassays for IFN? include cytopathic effect blocking, by viruses such as VSV, SFV and BMCV, on their target cells. A number of receptors for IFN? are now known and seem to be expressed on most cell types. 2-48 Is specific for human interferon ? 1 and does not cross react with human interferon ? 2.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
Feb-48
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Kontsek, P. et al. Mol Immunol. 29: 863-870 (1992)
CDw78 (also called Ba antigen, Leu21 or LO panB a) is present on some immature and some mature B-cells. The antigen appears on B-cell progenitors preceding CD10, CD19, CD22 and CD37. It is expressed on resting B-cells and reappears and persists in the cytoplasm and on the cell surface until cytoplasmic Ig appears. Its expression is greatly increased after B-cell activation in vitro. It is also found on tissue macrophages and on epithelial cells, but not on Tcells, NK-cells, monocytes, granulocytes, thymocytes or bone marrow stromal fibroblasts nor myeloid tissues.
Antibody Isotype:
IgG3-K
Monosan Range:
MONOSAN
Clone:
IPO-10
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Pinchouk V.G. et al, Anticancer Res. 8: 1377-1380 (1988)
HE-10 preferably reacts with determinants of chain A type 3 and 4 and chain H type 3 and 4, but not with type 1 and 2 chain structures. It is not reactive with immunodominant A trisaccharide. Increased expression of this antigen has been observed on some tumor tissues such as gastric carcinomas, urothelial carcinomas, and colon carcinomas. HE-10 does not react with normal tissue sections of donors with blood group B and 0 but it reacts specifically with malignant tissues in these individuals. HE-10 is applicable for red cell agglutination, tissue staining and immunofluorescence tests.
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
HE-10
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
N?mec M. et al. Vox Sang 52:125-8 (1987)
References 2:
Vanák J. et al. Neoplasma 36(4): 479-487 (1989)
References 3:
Tichý, M. et al. Neoplasma 37(4): 451-459 (1990)
References 4:
Tichý, M. et al. Acta Univ Palacki Olomuc Fac Med. 126: 57-69 (1990)
47-8D3 reacts with macrophages and detects the well-known leukocyte L1, cystic fibrosis antigen. Detecting a single protein band of 14 kDa in Western blots of lysates of human monocytes and granulocytes, the antigen was identified as the calcium-binding protein MRP14, which is a member of the S100 family involved a.o. in regulating the cell cycle. MRP14 is also implicated in the abnormal differentiation of myeloid cells in the stroma of cancer. It is further found on squamous mucosal epithelia. When associated with MRP8 it forms the heterodimer calprotectin.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
47-8D3
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Flavell DJ. et al., J. Histochem. Cytochem. 35: 1217-1226 (1987)
References 2:
Facchetti F. et al., Am. J. Clin. Pathol. 92: 42-50 (1989)
References 3:
Bardadin KA. et al., J. Pathol. 164: 253-259 (1991)
References 4:
Goebeler M. et al., J. Leukocyte Biol. 55: 259-261 (1994)
Laminins are large hetero-trimeric, non-collagenous glycoproteins found in basement membranes and composed of ?, ?, and ? chains. A5 reacts specifically with ? chain 1. Alterations of basement membrane integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
A5
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Ljubimov JY et. al. Cancer Res. 61(14): 5601-5610 (2001)
References 2:
Ljubimov AV et. al. Int J Cancer 50: 562-566 (1992)
C-46 reacts with keratins 7 (54kDa) and 17 (46kDa). On formalin-fixed paraffin embedded sections C46 reacts with keratin 7 only. Strongly positive on most simple epithelia except for stomach, small intestine and colon mucosa, hepatocytes, pancreatic acini, renal tubules, also positive on some non-cornifying epithelia.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
C-46
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bártek et al. J. of Pathol. 164: 215-24 (1991)
References 2:
Vojt?ek et al. Neoplasma 37(3): 333-342 (1990)
References 3:
Taylor Papadimitriou J. et. al. J. Cell Sci. 94: 403-13 (1989)
References 4:
Kova?ík et al. Int. J. Cancer Suppl. 3: 50-55, (1988)
References 5:
Kova?ík et al. J. Tumor Marker Oncol. 5: 219, (1990)
MHC class II molecules are encoded by polymorphic MHC genes and consist of a non-covalent complex of an ? and ? chain. Helper T lymphocytes bind antigenic peptides presented by MHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumulating in endosomal/lysosomal compartments and on the surface of B-cells, HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
EBS-O-111
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Thompson CJ et al., Human Immunol 6: 133-150 (1983)
The Neuropeptide Y Y1 Receptor was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat cortex, arcuate and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/500 - 1/1000 in PBS - Bn/Av-HRP detection.
The antibody was characterized by immunohistochemistry and Western blot. Western blot showed one immunoreactive band of 40 kD and a single high molecular weight band, presumably a precursor molecule. Preincubation of the antibody with an excess of the synthetic peptide blocked staining. Immunohistochemical staining of rat brain correlates well with Northern analysis, in situ hybridization and receptor autoradiography. BlastP database sequence homology searches confirmed that this sequence is unique to rat, mouse and human NPY Y1 receptors.
The 5-HT7 Receptor Antibody was raised against synthetic peptide sequence corresponding to amino acids 8-23 of the rat 5-HT7 receptor coupled to carrier protein with glutaraldehyde. The ImmunoStar 5-HT7 receptor antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat cortex and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/100 - 1/300 in PBS - biotin/avidin-HRP Technique. Note: use of Triton X-100 or other detergents is not recommended. The antibody was characterized by immunohistochemistry. Immunohistochemical staining of rat brain correlates well with Northern blot analysis, in situ hybridization and receptor autoradiography studies. Immunolabeling is completely abolished by preadsorption with synthetic rat 5-HT7 receptor (8-23). BlastP database sequence homology searches indicate that the amino acid sequence is unique to rat 5-HT7A, 5-HT7B and 5-HT7C. There is also significant sequence overlap with the mouse and human forms.
The 5-HT Antibody was raised in rabbit against 5-HTP coupled to BSA with paraformaldehyde. The antibody has a proven maximum biotin-streptavidin/HRP staining at a 1/1000 - 1/2000 dilution in rat raphe nuclei. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. The specificity of the antiserum was evaluated using a model system of gelatin-indole plugs by a method similar to published procedures (Shipper and Tilders, 1983). Results showed that the 5-HTP antibody dose dependently stained 5-HTP but did not stain any concentration of 5-HT or 5-HIAA. The antiserum was also tested by pre-adsorption with indole/paraformaldehyde/BSA conjugates. Staining was completely blocked by pre-adsorption with 5-HTP conjugate and unaffected by 5-HIAA or 5-HT conjugate.
The 5-HT 2C Receptor Antibody was raised against synthetic peptide sequence corresponding to amino acids 439-460 of the rat 5-HT2C receptor coupled to KLH and bovine thyroglobulin. The ImmunoStar 5-HT2C receptor antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat choroid plexus and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are /500 - 1/1000 in PBS - Bn/Av-HRP technique. Intensification methods such as nickel will approximately double the dilution factor as recommended. The antibody was characterized by immunohistochemistry and Western blot. Western blotting revealed a single band of approximately 70 kD. Preincubation of the antibody with an excess of the synthetic peptide blocked staining. Immunohistochemical staining of rat brain correlates well with Northern analysis, in situ hybridization and receptor autoradiography. BlastP database sequence homology searches confirmed that this sequence is unique to rat, mouse and human 5-HT2C receptors.
The GAT-2 antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat retina and leptomeninges using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/100-1/200 in PBS/0.3% Triton X-100Â - Cy3 Technique and 1/500 - 1/1,000 in PBS/0.3% Triton X-100 - biotin/avidin-HRP Technique. The antiserum has been characterized as specific to GAT-2; please see references listed below. GAT-2 immunolabeling is completely abolished by soluble pre-adsorption with synthetic rat GAT-2 (594-602) at a concentration of 10-5 M.
Raised against a C-terminal synthetic peptide sequence corresponding to amino acids 894-907 of rat GluR1 coupled to bovine thyroglobulin with gluraraldehyde.
Product Type:
Antibody - Antibodies
Antibody Type:
polyclonal
Format:
Lyophilised
Host Animal:
Rabbit
Immunogen:
Rat GluR1 (894-907)
Applications:
Immunohistochemistry, Immunocytochemistry, Western Blot
The GluR1 (lonotropic Glutamate Receptor1) Antibody was raised against a C-terminal synthetic peptide sequence corresponding to amino acids 894-907 of rat GluR1 coupled to bovine thyroglobulin with gluraraldehyde. The antibody produces strong labeling of GluR1 at dilutions of 1/4,000 - 1/6,000 using biotin-streptavidin peroxidase technique in rat cortex and hippocampus. Western blot analysis of GluR1 transfected cells and rat brain homogenates the antibody specifically labels a single band at Uapproximately 102 kD. Western blot analysis of GluR2, 3, 4, 4C, 5, 6, and 7 transfected cells revealed no immunolabeling. Immunolabeling of the above non-NMDA transfected cells demonstrates specificity for GluR1. Additionally, immunolabeling for GluR1 is completely abolished by pre-adsorption with synthetic rat GluR1 (894-907) at 5 µg per mL of diluted antibody.
The neuronal nitric oxide synthase C-terminal antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus, striatum, cortex and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/1,000 - 1/1,500 in PBS/0.3% Triton X-100 - Cy3 Technique and 1/8,000 - 1/12,000 in PBS/0.3% Triton X-100 - Bn/Av-HRP Technique. By Western blot analysis of brain homogenates the antibody specifically labels a band of approximately 155 kD. Immuno-labeling is completely abolished by pre-adsorption with synthetic human nNOS (1419-1433) at 5 µg per mL of diluted antibody. No cross reactivity with other forms of NOS were observed. The nNOS antiserum has been used successfully in human, rat, mouse, guinea pig, cat, and monkey tissue. Detection of nNOS from other species will depend upon sequence homology.
Raised against synthetic peptide sequence corresponding to amino acids 17-34 of the rat 5-HT5A receptor coupled to carrier protein with glutaraldehyde.
Product Type:
Antibody - Antibodies
Antibody Type:
polyclonal
Format:
Liquid
Host Animal:
Rabbit
Species Reactivity:
Clam, Rat
Immunogen:
Rat 5-HT5A receptor (17-34)
Applications:
Immunohistochemistry, Immunocytochemistry, immunofluorescence, Western Blot
The 5-HT 5A Receptor Antibody was raised against synthetic peptide sequence corresponding to amino acids 17-34 of the rat 5-HT5A receptor coupled to carrier protein with glutaraldehyde. The ImmunoStar 5-HT5A Receptor was quality control tested using standard immunohisto-chemical methods. The antiserum demonstrates strongly positive labeling of rat cortex and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/100 - 1/300 in PBS/0.3% Triton X-100Â - Cy3 and 1/200Â - 1/400 in PBS/0.3% Triton X-100Â - Bn/Av-HRP. Intensification methods such as nickel will approximately double the dilution factor as recommended. The antibody was characterized by immunoblotting and immunohistochemistry. Immunoblots of rat brain extracts revealed the presence of two bands at molecular weights of 41 and 47 kD. The lower weight band agrees with the calculated molecular weight based on amino acid sequence. The higher weight may represent glycosylated receptor protein. Immunohistochemical staining of rat brain correlates well with Northern blot analysis and in situ hybridization studies. Immunolabeling is completely abolished by preadsorption with synthetic rat 5-HT5A receptor (17-34). BlastP database sequence homology searches indicate that the amino acid sequence is unique to rat and mouse 5-HT5A receptor.
The Calretinin Antibody was raised to chick calretinin fusion protein. The antibody has a proven maximum biotin-avidin/HRP staining at a 1/2000 - 1/4000 dilution in rat cortex, hippocampus and hypothalamus.
The ImmunoStar N-terminal neuronal nitric oxide synthase antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus, striatum, cortex and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/1,000 - 1/2,000 in PBS/0.3% Triton X-100 - biotin/avidin-HRP. By Western blot analysis of brain homogenates the antibody specifically labels a band of approximately 155 kD. Immunolabeling is completely abolished by pre-adsorption with synthetic human nNOS (134-148) at 5 µg per mL of diluted antibody. No cross reactivity with other forms of NOS was observed.
The 5-HT 1A Receptor Antibody was raised against synthetic peptide sequence corresponding to amino acids 294-312 of the rat 5-HT1A receptor. The antiserum is provided as 100 µL of affinity purified serum containing 1% BSA. The antiserum demonstrates strongly positive labeling of rat cortex, arcuate and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/100 - 1/200 in PBS - Bn/Av-HRP detection. Intensification methods such as nickel will approximately double the dilution factor as recommended. The antibody was characterized by immunohistochemistry and Western blot. Western blot showed a single band of approximately 45 kD. Preincubation of the antibody with an excess of the synthetic peptide blocked staining. Immunohistochemical staining of rat brain correlates well with Northern analysis, in situ hybridization and receptor autoradiography. BlastP database sequence homology searches confirmed that this sequence is unique to rat, mouse and human 5-HT1A receptors.
The ImmunoStar 5HT 6-receptor antibody was quality control tested using standard immunohistochemical methods. The antiserum demonstrates significant labeling of rat cortex, amygdala and hippocampus and other areas using indirect immunofluorescent and biotin/avidin-HRP techniques. The addition of intensifying reagents such as nickel ammonium sulfate to the chromogen solution will approximately double the dilution factor as recommended. Immunolabeling is completely abolished by preadsorption with synthetic rat 5HT6 receptor (CLERPPGTPRHPPGPPLW). Immunolabeling of western blot revealed a single band of approximately 53kD.
The histochemical antibody for Vesicular Monoamine Trnasporter 2 (VMAT2) is generated in a rabbit from a synthetic peptide corresponding to rat VMAT2 496-515 coupled to carrier protein. The antiserum is provided as l00 µL of lyophilized whole serum.
Product Type:
Antibody - Antibodies
Antibody Type:
polyclonal
Format:
Lyophilised
Host Animal:
Rabbit
Species Reactivity:
Rat
Expected Species:
100% sequence homology with rat, 85% with mouse and 93% with human
Immunogen:
Synthetic peptide corresponding to rat VMAT2 496-515 coupled to carrier protein.
Applications:
Immunohistochemistry, Immunocytochemistry, Western Blot
The VMAT2 antiserum was quality control tested using standard immunohistochemical methods in rat brain and adrenal medulla using biotin/avidin-HRP techniques. Specificity of the antiserum was demonstrated by soluble pre-adsorption and Western blot. Tissue staining is completely eliminated by pretreatment of the diluted antibody with an excess of rat VMAT2 peptide residues 496-515. Western blot analysis of immunoprecipitated rat brain homogenates demonstrates a dense immunoreactive band of approximately 55 kD and a minor band of approximately 75 kD.
The histochemical antibody for Vesicular Monoamine Transporter 1 (VMAT1) is generated in a rabbit from a synthetic peptide corresponding to rat VMAT1 502-521 coupled to carrier protein. The antiserum is provided as l00 µL of lyophilized whole serum.
Product Type:
Antibody - Antibodies
Antibody Type:
polyclonal
Format:
Lyophilised
Host Animal:
Rabbit
Species Reactivity:
Rat
Expected Species:
100% sequence homology with rat, 90% with mouse and human
Immunogen:
Synthetic peptide corresponding to rat VMAT1 502-521 coupled to carrier protein.
Applications:
Immunohistochemistry, Immunocytochemistry, Western Blot
The VMAT1 antiserum was quality control tested using standard immunohistochemical methods in rat adrenal medulla using biotin/avidin-HRP techniques; the antiserum shows no reactivity in rat CNS. Specificity of the antiserum was demonstrated by soluble pre-adsorption and Western blot. Tissue staining is completely eliminated by pretreatment of the diluted antibody with an excess of rat VMAT1 peptide residues 502-521. Western blot analysis of immunoprecipitated rat adrenal homogenates demonstrates a dense immunoreactive band of approximately 55 kD and a minor band of approximately 75 kD.
The Beta-Endorphin Antibody was raised to synthetic human beta endorphin coupled to KLH with carbodiimide. The antibody produces a strong indirect immunofluorescent staining at a 1/200 - 1/400 dilution and a 4+ biotin-streptavidin/HRP staining at a 1/1000 - 1/2000 dilution in rat anterior pituitary. Staining is completely eliminated by pretreatment of the diluted antibody with 10-6 M of Ã-Endorphin. Pre-adsorption of the diluted antibody with 10-6M of the following substances had no effect on -Endorphin labeling: methionine enkephalin, leucine enkaphalin, dynorphin A, dynorphin B, gamma-endorphin, alpha-endorphin, ACTH and alpha-melanocyte stimulating hormone.
The Substance P antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat substantia nigra and spinal cord using biotin/avidin-HRP techniques. Recommended primary dilutions are 1/500-1/1000 in PBS/0.3% Triton X-100 - Cy3 Fluorchrome and 1/6000-1/8000 in PBS/0.3% Triton X-100 - biotin/avidin-HRP Technique. The specificity of the antiserum for Substance P was demonstrated using soluble pre-adsorption with the peptides in question at a final concentration of 10 µg of peptide per mL of diluted antiserum. Substance P immunolabeling was completely abolished by pre-adsorption with Substance P. Pre-adsorption with the following peptides resulted in no reduction of immunostaining: neurokinin A, neurokinin B, somatostatin and neuropeptide K.
The antibody has a proven strong indirect immunofluorescent staining at a 1/400-1/600 dilution and a proven 4+ biotin-streptavidin/HRP staining at 1/1,000-1/1,200 dilution in rat globus pallidus and amygdala. Staining is completely eliminated by pretreatment with 100 µg of methionine enkephalin per mL of diluted antiserum. Pretreatment with 100 µg of leucine enkephalin only partially blocks staining.
The antibody has a proven strong fluorescent staining at a 1/200-1/400 dilution and a strong Biotin-Streptavidin/HRP staining at a 1/4000-1/6000 dilution in rat amygdala, cortex, and suprachiasmatic nucleus. The specificity of the antiserum was examined by soluble pre-adsorption with the peptides in question at a final concentration of 10-5 M. VIP immunolabeling was completely abolished by pre-adsorption with VIP. Pre-adsorption with the following peptides resulted in no reduction of immunostaining: Secretin, gastric inhibitory polypeptide, somatostatin, glucagon, insulin, ACTH, gastrin 34, FMRF-amide, rat GHRF, human GHRF, peptide histidine isoleucine 27, rat pancreatic polypeptide, motilin, peptide YY, substance P, neuropeptide Y, and CGRP. The histochemical antibody for VIP is generated in a rabbit against porcine VIP conjugated to bovine thyroglobulin with carbodiimide. The antibody is provided as 100 µL of lyophilized whole serum, and 0.09% sodium azide.
The ACTH Antibody was raised to ACTH (1-39) purified from porcine pituitary. The antibody produces a maximum fluorescein staining at a 1/100 - 1/200 dilution and a 4+ biotin-streptavidin/HRP staining at a 1/500 - 1/1000 dilution in rat anterior/intermediate pituitary. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. The recommened dilution series are 1/100-1/200 in PBS/0.3% Triton X-100 - FITC Technique and 1/500-1/1000 in PBS/0.3% Triton X-100 - Bn-SA/HRP Technique. Staining is completely eliminated by pretreatment of the diluted antibody with 100 µg/mL of ACTH.
The antibody produces a strong postive labeling of LHRH at dilutions of 1/200-1/400 using indirect immunofluorescence and at dilutions of 1/2,000 - 1,4,000 using biotin-streptavidin/HRP in rat hypothalamus (median eminence). Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. Staining is completely eliminated by pretreatment of the diluted antibody with 5 µg of LHRH per mL of diluted antiserum.
The 5-HT Rabbit Antibody was raised against serotonin coupled to BSA with paraformaldehyde. The ImmunoStar serotonin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus, raphe nuclei and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/1,000-1/2,000 in PBS/0.3% Triton X-100 - Cy3 Technique and 1/20,000-1/40,000 in PBS/0.3% Triton X-100 - biotin/avidin-HRP Technique. Staining is completely eliminated by pretreatment of the diluted antibody with 25 ug of serotonin/BSA. Cross reactivity of Serotonin antisera was examined. With 5µg, 10µg and 25µg amounts the following substances did not react with Serotonin antisera diluted 1/20,000 using the Bn-SA/HRP labeling method: 5-hydroxytryptophan, 5-hydroxyindole -3- acetic acid, and dopamine.
The antibody has a proven strong fluorescent staining at a 1/400 - 1/800 dilution and a proven strong biotin-streptavidin/HRP staining at a 1/2000 - 1/4000 dilution in rat hypothalamus. Staining is completely eliminated by pretreatment of the diluted antibody with 10 µg/mL of arginine vasopressin. Pre-adsorption with as much as 100 µg/mL of oxytocin had no effect on immunolabeling.
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