GRF (General regulatory factor 2) or 14.3.3’s are 30KDa proteins involved in protein interactions with target proteins containing phosphorylated target sites. Functions of 14.3.3’s include acting as adaptors or scaffolds, stimulating protein-protein interaction, altering target protein activity, causing conformational changes of target proteins, regulating subcellular localisation and also facilitating transport (for example nuclear import/export and transport in the endomembrane system). A variety of apparently unrelated biological activities, including a role in development and signal transduction - brassinosteroid mediated signaling pathway.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
This product can be sold containing proclin if requested.
Application Details:
1: 2000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 µl of sterile water.
Related products:
AS08 317 | 14.3.3C | 14.3.3-like protein C, rabbit antibody specific to Hordeum vulgareMatching secondary antibody for chemiluminescence detectionPlant protein extraction buffer Secondary antibodies | AgriseraSuperDeal
Molecular Weight:
20-28 kDa (depending upon an isoform)
Not reactive in:
This antibody does not bind to 14-3-3-like protein D of Nicotiana tabacum.
Selected references:
Gupta and Shaw (2020). Biochemical and molecular characterisations of salt tolerance components in rice varieties tolerant and sensitive to NaCl: the relevance of Na+ exclusion in salt tolerance in the species . Funct Plant Biol. 2020 Jul 30.doi: 10.1071/FP20089 Dongxu et al. (2020). Magnesium reduces cadmium accumulation by decreasing the nitrate reductase-mediated nitric oxide production in Panax notoginseng roots. Journal of Plant Physiology. Available online 7 February 2020, 153131Pertl-Obermeyer et al. (2018). Dissecting the subcellular membrane proteome reveals enrichment of H+ (co-)transporters and vesicle trafficking proteins in acidic zones of Chara internodal cells. PLoS One. 2018 Aug 29;13(8):e0201480. doi: 10.1371/journal.pone.0201480. Obroucheva (2017). Participation of Plasma Membrane H+-ATPase in Seed Germination. Internat. J. of Cell Science & Molecular Biol. Vol. 2 Issue 3. DOI : 10.19080/IJCSMB.2017.02.555589.Barkla et al. (2016). Single-cell-type quantitative proteomic and ionomic analysis of epidermal bladder cells from the halophyte model plant Mesembryanthemum crystallinum to identify salt-responsive proteins. BMC Plant Biol. 2016 May 10;16(1):110. doi: 10.1186/s12870-016-0797-1.
Special application note:
This antibody is recognizing recombiant GRF of Lilium longiflorum Lil1433_0 accession: AF191746, Lil1433_2, accession: EF397608 and recombinant GRF1,2,3,5 and 6 of Arabidopsis thaliana: GRF1 14-3-3 chi (At4g09000.1), GRF2 14-3-3 omega (At1g78300.1), GRF3 14-3-3 psi (At5g38480.1), GRF5 14-3-3 upsilon (At5g16050.1), GRF6 14-3-3 lambda (At5g10450.2), GRF8 14-3-3 kappa (At5g65430.1), GRF11 14-3-3 omicron (At1g34760.1).There is also very weak reaction to Physcomitrella patens Pp14-3-3 Pp1s 73_133V6 (closest homolog to AtGRF6) and Chlamydomonas reinhardtii Cr 14-3-3 Cre 12.g559250 (closest homolog to AtGRF6).
ABF3 (Abscisic acid-insensitive 5-like 6) binds to the ABA-responsive element (ABRE) and mediates stress-responsive ABA signaling.Alternative names: Abscisic acid responsive elements-binding factor 3, ABRE-binding factor 3, Dc3 promoter-binding factor 5, AtDPBF5, bZIP transcription factor 37, AtbZIP37
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
ABI5 (abscisic acid insensitive 5) is involved in ABA-regulated gene expression during seed development and subsequent vegetative stage and acts as the major mediator of ABA repression of growth. Binds to the embryo specification element and the ABA-responsive element (ABRE) of the Dc3 gene promoter and to the ABRE of the Em1 and Em6 genes promoters. Alternative names: ABI5, ABA INSENSITIVE 5, GIA1, GROWTH-INSENSITIVITY TO ABA 1, Dc3 promoter-binding factor 1, AtDPBF1, GROWTH-INSENSITIVITY TO ABA 1, bZIP transcription factor 39, AtbZIP39.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica oleracea Species of your interest not listed? Contact us
Immunogen:
Recombinant HIS-tagged, full length ABI5 in gel slice, of Arabidopsis thaliana UniProt: Q9SJN0-1, TAIR: At2g36270, overexpressed in E.coli
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Stone et al. (2006). KEEP ON GOING, a RING E3 ligase essential for Arabidopsis growth and development, is involved in abscisic acid signaling. Plant Cell. 2006 Dec;18(12):3415-28.
ACC (1-Aminocyclopropane-1-carboxylic acid) plays an important role in the biosynthesis of the plant hormone ethylene. It is synthesized by the enzyme ACC synthase ( EC 4.4.1.14) from methionine and converted to ethylene by ACC oxidase (EC 1.14.17.4).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Details of used immunogold protocol are provided under an image.
Application Details:
1 : 100 (IG)
Purity:
Serum
Reconstitution:
For reconstitution add 200 µl of sterile water.
Related products:
AS14 2774 | Anti-ACC synthase 7 | 1-aminocyclopropane-1-carboxylate synthase 7, rabbit antibodiescollection of antibodies against plant hormonesPlant and algal protein extraction buffer Secondary antibodies | AgriseraSuperDeal
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Wilmowicz et al. (2019). Abscisic acid and ethylene in the control of nodule-specific response on drought in yellow lupine. Environmental and Experimental Botany Available online 2 October 2019, 103900.Serova et al. (2018). Early nodule senescence is activated in symbiotic mutants of pea (Pisum sativum L.) forming ineffective nodules blocked at different nodule developmental stages. Protoplasma. 2018 Apr 3. doi: 10.1007/s00709-018-1246-9.
1-aminocyclopropane-1-carboxylate synthase (ACS) enzymes catalyze the conversion of S-adenosyl-L-methionine (SAM) into 1-aminocyclopropane-1-carboxylate (ACC), a direct precursor of ethylene. (EC:4.4.1.14). Synonymes: S-adenosyl-L-methionine methylthioadenosine-lyase 7.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Acd1 (accelerated cell death 1) EC=1.14.12.20 is an enzyme involved in chlorophyll breakdown, pheophorbide a oxygenase. This enzyme seems to induce cell death in Arabidopsis leaves in the dark. Synonymes:PaO, Lls1, lethal leaf-spot 1 homolog, pheide a oxygenase
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica napus, Solanum lycopersicum, Nicotiana tabacum Species of your interest not listed? Contact us
Immunogen:
Recombinant PaO from Arabidopsis thaliana Q9FYC2, At3g44880
This antibody works on total cell extracts and can be used as a senescence marker. Predicted size of Acd1 precursor protein is about 61 kD including the transit peptide, but it must be processed to a smaller size. Using fresh extracts is recommended to decrease possible cross-reaction with Rubisco.
Application Details:
1 : 5000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 µl of sterile water.
Related products:
collection of antibodies to proteins involved in senescencePlant protein extraction buffer Secondary antibodies | AgriseraSuperDeal
Molecular Weight:
61 | 54 kDa
Not reactive in:
Pinus strobus
Selected references:
Kim et al. (2013). Mutation of the Arabidopsis NAC016 Transcription Factor Delays Leaf Senescence.'Plant Cell Physiol. Aug 21.Nagane et al. (2010). Involvement of AtNAP1 in thre reulation of chlorophyll degradation in Arabiopsis thaliana. Planta (4):939-949.Hirashima et al. (2009). Light-independent cell death induced by accumulation of pheophorbide a in Arabidopsis thaliana. Plant Cell Physiol. (4):719-729.
Special application note:
The protein level is moderately induced during dark-induced senescence.
Aconitase is a single subunit enzyme of the tricarboxylic acid cycle (or Krebs cycle) in the mitochondria. A cytosolic isoform is also part of the glyoxylate cycle. Aconitase catalyzes the dehydration / hydration of citrate to iso-citrate, via cis-aconitate as an intermediate. The reaction is facilitated by an iron-sulphur cluster in the active site of the enzyme. The iron-sulphur cluster is somewhat unstable, especially under oxidative stress, and loss of the cofactor leads to degradation of the protein.Alternative names: ACO, citrate hydro-lyase 1,2,3
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana ACO1,ACO2 and ACO3 isoforms, Brassica oleracea, Solanum lycopersicum
Expected Species:
Cucurbita maxima, Nicotiana tabacum, Ricinus communis, Solanum tuberosum, Vitis vinifera, Oryza sativa, Zea mays, Picea sitcHensis, Populus trichocarpa Species of your interest not listed? Contact us
Immunogen:
Arabidopsis ACO1 (AT4G35830, Q42560), codon 120 – 898 (C-terminus), was cloned in fusion with a N-terminal 6xHis tag, and over-expressed in E. coli. All recombinant protein accumulated in inclusion bodies, which were purified by centrifugation and solubilised in 6 M guanidine-HCl. The protein was refolded by dilution in 100 mM Tris-HCl 8.5, 10% (v/v) glycerol, 2 mM dithiothreitol, and concentrated prior to immunisation.
The antibody recognises all three Arabidopsis aconitase isoforms (ACO1, ACO2 and ACO3, see Bernard et al 2009). Possible differences in affinity have not been precisely quantified. Sensitivity threshold is between 2 and 10 ng for WB / ECL (see figure). Antibodies will recognize aconitase isoforms in denaturing and native gel electrophoresis.
Application Details:
1 : 5 000 -1 : 10 000 (WB). At higher concentrations the antibody binds aspecifically resulting in non-specific signals around 60 kDa, including Rubisco subunits.
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water.
Related products:
collection of antibodies to proteins located in mitochondriaPlant protein extraction bufferSecondary antibodies | AgriseraSuperDeal
Molecular Weight:
98 kDa. Note that ACO1, ACO2 and ACO3 cannot be distinguished in size by standard SDS-PAGE.
Not reactive in:
Chlamydomonas reinhardii, cyanobacteria
Selected references:
Rurek et al. (2018). Mitochondrial Biogenesis in Diverse Cauliflower Cultivars under Mild and Severe Drought Involves Impaired Coordination of Transcriptomic and Proteomic Response and Regulation of Various Multifunctional Proteins. Preprints 2018, 2018010276 (doi: 10.20944/preprints201801.0276.v1).Setién et al. (2014). Root phosphoenolpyruvate carboxylase and NAD-malic enzymes activity increase the ammonium-assimilating capacity in tomato. J Plant Physiol. 171:49-63.Birke et al. (2012). Cysteine biosynthesis, in concert with a novel mechanism, contributes to sulfide detoxification in mitochondria of Arabidopsis thaliana. Biochem J. May 2, ahead of print.
Special application note:
Arabidopsis expresses three highly similar aconitase isozymes (ACO1/ AT4G35830, ACO2/AT4G26970 and ACO3/AT2G05710), of which ACO1 is the cytosolic isoform, while ACO2 and ACO3 are predominantly located in the mitochondria (Arnaud et al 2007, Bernard et al 2009). The combined abundance and activity of the mitochondrial aconitases is about 3 times higher than the cytosolic pool (Bernard et al 2009).The Arabidopsis isoforms are more similar in amino acid sequence to mammalian iron-regulatory proteins (IRP-1) than to the mammalian and yeast mitochondrial aconitases.
Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Chlamydomonas reinhardtii (too high background for this species)
Selected references:
Khajuria et al. (2020). Photochemical Efficiency Is Negatively Correlated With the Δ 9- Tetrahydrocannabinol Content in Cannabis Sativa L. Plant Physiol Biochem. 2020 Apr 8;151:589-600. doi: 10.1016/j.plaphy.2020.04.003.Molnár et al. (2020). Nitro-oxidative Signalling Induced by Chemically Synthetized Zinc Oxide Nanoparticles (ZnO NPs) in Brassica Species. Chemosphere, 251, 126419 Roustan et al. (2020). Protein sorting into protein bodies during barley endosperm development is putatively regulated by cytoskeleton members, MVBs and the HvSNF7s. Sci Rep. 2020 Feb 5;10(1):1864. doi: 10.1038/s41598-020-58740-x.Dalmadi et al. (2019). AGO-unbound cytosolic pool of mature miRNAs in plant cells reveals a novel regulatory step at AGO1 loading. Nucleic Acids Res. 2019 Aug 8. pii: gkz690. doi: 10.1093/nar/gkz690.Patankar et al. (2019). Functional Characterization of Date Palm Aquaporin Gene PdPIP1;2 Confers Drought and Salinity Tolerance to Yeast and Arabidopsis. Genes (Basel). 2019 May 22;10(5). pii: E390. doi: 10.3390/genes10050390.Scherer et al. (2019). Pulsed electric field (PEF)-assisted protein recovery from Chlorella vulgaris is mediated by an enzymatic process after cell death. Algal Research, Volume 41, August 2019, 101536.Czobor et al. (2019). Comparison of the response of alternative oxidase and uncoupling proteins to bacterial elicitor induced oxidative burst. PLoS One. 2019 Jan 10;14(1):e0210592. doi: 10.1371/journal.pone.0210592.Deng et al. (2019). Integrated proteome analyses of wheat glume and awn reveal central drought response proteins under water deficit conditions. J Plant Physiol. 2019 Jan;232:270-283. doi: 10.1016/j.jplph.2018.11.011.Wang et al. (2018). A role of GUNs-Involved retrograde signaling in regulating Acetyl-CoA carboxylase 2 in Arabidopsis. Biochem Biophys Res Commun. 2018 Nov 2;505(3):712-719. doi: 10.1016/j.bbrc.2018.09.144.Borovik and Grabelnych (2018). Mitochondrial alternative cyanide-resistant oxidase is involved in an increase of heat stress tolerance in spring wheat. J Plant Physiol. 2018 Dec;231:310-317. doi: 10.1016/j.jplph.2018.10.007.Pan et al. (2018). Comparative proteomic investigation of drought responses in foxtail millet. BMC Plant Biol. 2018 Nov 29;18(1):315. doi: 10.1186/s12870-018-1533-9.López‐Calcagno et al. (2018). Overexpressing the H‐protein of the glycine cleavage system increases biomass yield in glasshouse and field‐grown transgenic tobacco plants. Plant Biotechnology Journal, May 2018. Li et al. (2018). Comparative proteomic analysis of key proteins during abscisic acid-hydrogen peroxide-induced adventitious rooting in cucumber (Cucumis sativus L.) under drought stress. Journal of Plant Physiology Volume 229, October 2018, Pages 185-194.Adhikari et al. (2018). Sulfate improves cadmium tolerance by limiting cadmium accumulation, modulation of sulfur metabolism and antioxidant defense system in maize. Environmental and Experimental Botany Volume 153, September 2018, Pages 143-162.Brandt et al. (2018). Extended darkness induces internal turnover of glucosinolates in Arabidopsis thaliana leaves. PLoS One. 2018 Aug 9;13(8):e0202153. doi: 10.1371/journal.pone.0202153.Jespersen et al. (2017). Metabolic Effects of Acibenzolar-S-Methyl for Improving Heat or Drought Stress in Creeping Bentgrass. Front Plant Sci. 2017 Jul 11;8:1224. doi: 10.3389/fpls.2017.01224. eCollection 2017. (western blot, Agostis stolonifera cv. ‘Penncross’)Qiu et al. (2015). Soy 14-3-3 protein SGF14c, a new regulator of tolerance to salt–alkali stress. Plant Biotechnology Reports pp 1-9.Shaw et al. (2015). β-aminobutyric acid mediated drought stress alleviation in maize (Zea mays L.). Environ Sci Pollut Res Int. 2015 Sep 29.Buxa et al. (2015). Phytoplasma infection in tomato is associated with re-organization of plasma membrane, ER stacks, and actin filaments in sieve elements. ront Plant Sci. 2015; 6: 650. Published online 2015 Aug 19.Zheng et al. (2014). iTRAQ-based quantitative proteomics analysis revealed alterations of carbohydrate metabolism pathways and mitochondrial proteins in a male sterile cybrid pummelo. J Proteome Res. 2014 May 13.
Special application note:
Antibody available in 3 various pack sizes: 50, 100 and 150 µl - Please inquire.This product can be sold containing ProClin if requested.
Alcohol dehydrogenase (ADH) is an enzyme playing a crucial role in the fermentative metabolism in plants subjected to low oxygen stress. It is known to be synthesized preferentially under low oxygen conditions.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Allyl alcohol dehydrogenase of Nicotiana tabacum, accession 75206691 and in Chlamydomonas reinhardtii.
Selected references:
Gil-Monreal et al. (2019). ERF-VII transcription factors induce ethanol fermentation in response to amino acid biosynthesis-inhibiting herbicides. J Exp Bot. 2019 Aug 6. pii: erz355. doi: 10.1093/jxb/erz355.Bui et al. (2019). Conservation of ethanol fermentation and its regulation in land plants. J Exp Bot. 2019 Feb 28. pii: erz052. doi: 10.1093/jxb/erz052.De la Rosa et al. (2019), A dicistronic precursor encoding miR398 and the legume-specific miR2119 coregulates CSD1 and ADH1 mRNAs in response to water deficit. Plant Cell Environ. 2019 Jan;42(1):133-144. doi: 10.1111/pce.13209.Giuntoli et al. (2014). A trihelix DNA binding protein counterbalances hypoxia-responsive transcriptional activation in Arabidopsis. PLoS Biol. 2014 Sep 16;12(9):e1001950. doi: 10.1371/journal.pbio.1001950. eCollection 2014.
Special application note:
This product can be sold containing ProClin if requested.
Alcohol dehydrogenase (E.C.:1.1.1.1) is an important enzyme for plants and microbes. In microalgae and bacteria the conversion of Acetyl-CoA to ethanol under conditions of oxygen deprivation is catalyzed by the dual function enzyme alcohol/acetaldehyde dehydrogenase (ADH/ALDH; E.C.:1.1.1.1 /1.2.1.10). This reaction results in NAD+ recycling and allows glycolysis to proceed.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Kurylo et al. (2018). Endogenous rRNA Sequence Variation Can Regulate Stress Response Gene Expression and Phenotype. Cell Rep. 2018 Oct 2;25(1):236-248.e6. doi: 10.1016/j.celrep.2018.08.093.Laurenceau et al. (2015). Conserved Streptococcus pneumoniae Spirosomes Suggest a Single Type of Transformation Pilus in Competence. PLoS Pathog. 2015 Apr 15;11(4):e1004835. doi: 10.1371/journal.ppat.1004835.Kukuczka et al. (2014). Proton Gradient Regulation5-Like1-Mediated Cyclic Electron Flow Is Crucial for Acclimation to Anoxia and Complementary to Nonphotochemical Quenching in Stress Adaptation. Plant Physiol. 2014 Jun 19;165(4):1604-1617.
Special application note:
Selected peptide is well conserved in Escherichia coli ADHE (P0A9Q7), most of the microbial dual function aldehyde/alcohol dehydrogenases (ADHE) and Iron-containing alcohol dehydrogenases are also conserved in a peptide used to elicit ADH antibody.
AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure. Buffer for extraction of AGO proteins: Paudel et al. 2018. The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.TCA acetone precipitation method
Dunker et al. (2020). Oomycete small RNAs bind to the plant RNA-induced silencing complex for virulence. Elife. 2020 May 22;9:e56096.doi: 10.7554/eLife.56096. Niedojadlo et al. (2020). Dynamic distribution of ARGONAUTE1 (AGO1) and ARGONAUTE4 (AGO4) in Hyacinthus orientalis L. pollen grains and pollen tubes growing in vitro. Protoplasma. 2020 Jan 8. doi: 10.1007/s00709-019-01463-2.You et al. (2019). FIERY1 promotes microRNA accumulation by suppressing rRNA-derived small interfering RNAs in Arabidopsis. Nat Commun. 2019 Sep 27;10(1):4424. doi: 10.1038/s41467-019-12379-zDalmadi et al. (2019). AGO-unbound cytosolic pool of mature miRNAs in plant cells reveals a novel regulatory step at AGO1 loading. Nucleic Acids Res. 2019 Aug 8. pii: gkz690. doi: 10.1093/nar/gkz690.Li (2019). The Isolation of Total and Membrane-Bound Polysomes from Arabidopsis and the Detection of Their Associated AGO1 and sRNAs. Methods Mol Biol. 2019;1932:317-333. doi: 10.1007/978-1-4939-9042-9_23.Sprunck et al. (2019). Elucidating small RNA pathways in Arabidopsis thaliana egg cells. http://dx.doi.org/10.1101/525956Chen et al. (2018). Structural and biochemical insights into small RNA 3' end trimming by Arabidopsis SDN1. Nat Commun. 2018 Sep 4;9(1):3585. doi: 10.1038/s41467-018-05942-7. (Immunoprecipitation)Bologna et al. (2018). Nucleo-cytosolic Shuttling of ARGONAUTE1 Prompts a Revised Model of the Plant MicroRNA Pathway. Mol Cell. 2018 Feb 15;69(4):709-719.e5. doi: 10.1016/j.molcel.2018.01.007.Zhang et al. (2017). RISC-interacting clearing 3'- 5' exoribonucleases (RICEs) degrade uridylated cleavage fragments to maintain functional RISC in Arabidopsis thaliana. Elife. 2017 May 2;6. pii: e24466. doi: 10.7554/eLife.24466.Schalk et al. (2017). Small RNA-mediated repair of UV-induced DNA lesions by the DNA DAMAGE-BINDING PROTEIN 2 and ARGONAUTE 1. Proc Natl Acad Sci U S A. 2017 Mar 21. pii: 201618834. doi: 10.1073/pnas.1618834114.Dolata et al. (2016). Salt Stress Reveals a New Role for ARGONAUTE1 in miRNA Biogenesis at the Transcriptional and Posttranscriptional Levels. Plant Physiol. 2016 Sep;172(1):297-312. doi: 10.1104/pp.16.00830. (ChIP and Immunolocalization)Pumplin et al. (2016). DNA Methylation Influences the Expression of DICER-LIKE4 Isoforms, Which Encode Proteins of Alternative Localization and Function. The Plant Cell November 14, 2016 tpc.00554.2016 . Advance Publication November 14, 2016.Minoia et al. (2014). Specific Argonautes Selectively Bind Small RNAs Derived from Potato Spindle Tuber Viroid and Attenuate Viroid Accumulation In Vivo. J Virol. 2014 Oct 15;88(20):11933-45. doi: 10.1128/JVI.01404-14. Epub 2014 Aug 6.Haveckeret al. (2010). The Arabidopsis RNA-directed DNA methylation argonautes functionally diverge based on their expression and interaction with target loci. Plant Cell. 2010 Feb;22(2):321-34. doi: 10.1105/tpc.109.072199. (small-RNA-IP-Seq)
Special application note:
Antibody binds microRNA and tasiRNAs, preference for 21nt miRNAs with 5'U.To detect AGO1 in Nicotiana benthamiana, please inquire.Recommended for detection of AGO1: extreme low femtogram range chemiluminescent detection reagent
AGO10 is involved in miRNA binding, and in RNA-mediated posttranscriptional gene silencing (PTGS).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
A. lyrata, B. napus, C. rubella, C. clementina, C. sinensis, E. salsugineum, G. arboreum, G. raimondii. N. benthamianaSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana AGO10 protein sequence, Uniprot: Q9XGW1, TAIR: AT5G43810
AGO expression may be cell/tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Seedlings can be used as a negative control. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.
AGO2 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi). AGO2 is probably involved in antiviral RNA silencing.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Capsella rubella, Solanum tuberosum Species of your interest not listed? Contact us
AGO2 protein is strongly induced by stress. AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure. Antibody incubation should be done over night in 4°C. Use of material with enriched AGO2 levels is recommened.
Wang et al. (2019). The PROTEIN PHOSPHATASE4 Complex Promotes Transcription and Processing of Primary microRNAs in Arabidopsis. Plant Cell. 2019 Feb;31(2):486-501. doi: 10.1105/tpc.18.00556.Dalmadi et al. (2019). AGO-unbound cytosolic pool of mature miRNAs in plant cells reveals a novel regulatory step at AGO1 loading. Nucleic Acids Res. 2019 Aug 8. pii: gkz690. doi: 10.1093/nar/gkz690.You et al. (2019). FIERY1 promotes microRNA accumulation by suppressing rRNA-derived small interfering RNAs in Arabidopsis. Nat Commun. 2019 Sep 27;10(1):4424. doi: 10.1038/s41467-019-12379-z. (immunoprecipiation)
AGO4 (Argonaute 4) belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur adhering to the cap or sides of the tubes.
AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure. Use a 6 % gel for protein separation, which is run longer to avoid a cross-reactivity at ca. 40 kDa.Binds endogenous siRNAs, preference for 24nt siRNAs with 5' A.Note that the AGO4 antibody reacts with the NEB prestained protein marker.
Application Details:
1 : 100 (ICC), 5 µg of antibody per 1 gram of a fresh tissue (IP),1 : 2000-1 : 5000 (WB)
NiedojadÃ??o et al. (2020). Dynamic distribution of ARGONAUTE1 (AGO1) and ARGONAUTE4 (AGO4) in Hyacinthus orientalis L. pollen grains and pollen tubes growing in vitro. Protoplasma. 2020 Jan 8. doi: 10.1007/s00709-019-01463-2.Sprunck et al. (2019). Elucidating small RNA pathways in Arabidopsis thaliana egg cells. http://dx.doi.org/10.1101/525956Yang et al. (2017). The developmental regulator PKL is required to maintain correct DNA methylation patterns at RNA-directed DNA methylation loci. Genome Biol. 2017 May 31;18(1):103. doi: 10.1186/s13059-017-1226-y.Li et al. (2016). Biogenesis of phased siRNAs on membrane-bound polysomes in Arabidopsis. Elife. 2016 Dec 12;5. pii: e22750. doi: 10.7554/eLife.22750.Zhai et al. (2015). A One Precursor One siRNA Model for Pol IV-Dependent siRNA Biogenesis. Cell. 2015 Oct 8;163(2):445-55. doi: 10.1016/j.cell.2015.09.032.Han et al. (2014). SUVR2 is involved in transcriptional gene silencing by associating with SNF2-related chromatin-remodeling proteins in Arabidopsis. Cell Res. 2014 Nov 25. doi: 10.1038/cr.2014.156. (coimmunoprecipitation)Zhang et al. (2013). DTF1 is a core component of RNA-directed DNA methylation and may assist in the recruitment of Pol IV. PNAS, May 14.Havecker et al. (2010) The RNA-directed DNA methylation Arabidopsis Argonautes functionally diverge based on expression and interaction with target loci. Plant Cell 22(2): 321-34.
AGO5 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi). Probably involved in antiviral RNA silencing.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
peptide of Arabidopsis thaliana AGO5 UniProt: Q9SJK3, TAIR:AT2G27880
Applications:
Immunofluorescebce (IF), Immunoprecipitation (IP), Western blot (WB)
AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.
Application Details:
1 : 100 (IF), 5 ug per gram floral tissue (IP), 1 : 1500 (WB)
AGO6 (Argonaute 6) belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide derived from Arabidopsis thaliana AGO6 UniProt: O48771 TAIR:At2g32940
Sprunck et al. (2019). Elucidating small RNA pathways in Arabidopsis thaliana egg cells. http://dx.doi.org/10.1101/525956 Havecker el al. (2010). The RNA-directed DNA methylation Arabidopsis Argonautes functionally diverge based on expression and interaction with target loci. Plant Cell. 2010 Feb;22(2):321-34. doi: 10.1105/tpc.109.072199.
Special application note:
There can be a reaction with NEB broad range prestained protein ladder depending upon secondary antibody used.AGO6 accumulates to lower levels in the nrpd1a mutant (Havecker at el. 2010). In addition, a non-specific band migrates to a similar location and thus protein was run on a 6% denaturing gel to obtain good separation.If other secondary antibody is used than in the figure below, cross-reacting pattern can be different.
AGO9 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana AGO9 protein sequence UniProt: Q84YI4, TAIR:At5g21150.
Applications:
Immunolocalization, whole-mount (IL), Immunoprecipitation (IP), Western blot (WB)
AGO expression may be cell/tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Seedlings can be used as a negative control. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.
Application Details:
5 µg of antibody per 1 gram of a fresh tissue (IP), 1 : 10 000 (WB)
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Sprunck et al. (2019). Elucidating small RNA pathways in Arabidopsis thaliana egg cells. http://dx.doi.org/10.1101/525956Su et al. (2017). The THO Complex Non-Cell-Autonomously Represses Female Germline Specification through the TAS3-ARF3 Module. Curr Biol. 2017 Jun 5;27(11):1597-1609.e2. doi: 10.1016/j.cub.2017.05.021.Havecker et al. (2010) The RNA-directed DNA methylation Arabidopsis Argonautes functionally diverge based on expression and interaction with target loci. Plant Cell 22(2): 321-34.
AHK2 (Histidine kinase 2) is a cytokinin receptor, multi-pass membrane protein which transmits the stress signal to a downstream MAPK cascade. Alternative name: Authentic HIS-Kinase 2.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Nicotiana benthamiana, Vitis viniferaSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana AHK2 protein sequence, TAIR: AT5G35750, UniProt: Q9C5U2
AKIN beta gamma is a regulatory subunit of SNF1-related protein kinase (SnRK) complex. Involved in signal transduction cascade in carbohydrate metabolism. Alternative names: ATSNF4, homolog of yeast sucrose nonfermenting 4, KIN??, SNF4.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
This antibody is recognizing well recombinant AKIN beta gamma as well as the over-expressed protein in protoplasts. It has not been confirmed working on endogenous cell extract.
AKIN10 (E.C.= 2.7.11.1) is a catalytic subunit of the putative trimeric SNF1-related protein kinase (SnRK) complex, which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants. Synonymes: AKIN alpha-2
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated synthetic peptide derived from C-terminal part of Arabidopsis thaliana AKIN10 sequence UniProt: Q38997, TAIR: At3g01090
AKIN10 antibody works best when first the SnRK1 complex is immunorecipitated with this or another antibody and then anti-AKIN10 antibody is used to probe with. When this antibody is used against a lysate, the dominant rubisco protein can mask any immunoreactivity.
Application Details:
1: 50 (IL), 1 : 500 (WB)
Purity:
Affinity purified serum in PBS, pH 7.4
Reconstitution:
For reconstitution add 50 µl/tube of sterile water.
Related products:
AS09 460 | Anti-AKINB1 | SNF1-related protein kinase regulatory subunit beta-1, rabbit antibodiesAS09 462 | Anti-AKINB2 | SNF1-related protein kinase regulatory subunit beta-2, rabbit antibodiesAS15 2859 | Anti-AKIN ß3 | SNF1-related protein kinase regulatory subunit beta-3, rabbit antibodiesAS09 463 | Anti-AKIN beta gamma, rabbit antibodiesAS10 920 | Anti-AKIN11 | SNF1-related protein kinase catalytic subunit alpha KIN11, rabbit antibodiesAS09 613 | Anti-AKING1 | SNF1-related protein kinase regulatory subunit gamma 1, rabbit antibodiescollection of antibodies to proteins involved in signal transductionPlant protein extraction buffer | AgriseraSuperDeal
Krasnoperova et al. (2019). Potential Involvement of KIN10 and KIN11 Catalytic Subunits of the SnRK1 Protein Kinase Complexes in the Regulation of Arabidopsis γ-Tubulin. Cytology and Genetics, Volume 53, Issue 5, pp 349–356. (immunolocalization)Blanco et al. (2019). Dual and dynamic intracellular localization of Arabidopsis thaliana SnRK1.1. J Exp Bot. 2019 Apr 15;70(8):2325-2338. doi: 10.1093/jxb/erz023.Frank et al. (2018). Circadian Entrainment in Arabidopsis by the Sugar-Responsive Transcription Factor bZIP63. Curr Biol. 2018 Aug 20;28(16):2597-2606.e6. doi: 10.1016/j.cub.2018.05.092.Chan et al. (2017). SnRK1 phosphorylation of FUSCA3 positively regulates embryogenesis, seed yield, and plant growth at high temperature in Arabidopsis. Journal of Experimental Botany, erx233, https://doi.org/10.1093/jxb/erx233.Nukarinen et al. (2016). Quantitative phosphoproteomics reveals the role of the AMPK plant ortholog SnRK1 as a metabolic master regulator under energy deprivation. Sci Rep. 2016 Aug 22;6:31697. doi: 10.1038/srep31697.Castro et al. (2015). SIZ1-Dependent Post-Translational Modification by SUMO Modulates Sugar Signalling and Metabolism in Arabidopsis thaliana. Plant Cell Physiol. 2015 Oct 14. pii: pcv149.Emanuelle et al. (2015). SnRK1 from Arabidopsis thaliana is an atypical AMPK. Plant J. 2015 Mar 3. doi: 10.1111/tpj.12813.Rodrigues et al. (2013). ABI1 and PP2CA Phosphatases Are Negative Regulators of Snf1-Related Protein Kinase1 Signaling in Arabidopsis. Plant Cell, Oct 31.
AKIN11 (E.C.= 2.7.11.1) is a catalytic subunit of the putative trimeric SNF1-related protein kinase (SnRK) complex, which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants. Synonymes: AKIN alpha-1
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
AKIN beta-1 (AKINB1) is a member of SnRK1/SNF1/AMPK family in plants. It is a regulatory subunit of the putative trimeric SNF1-related protein kinase (SnRK) comples which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide derived from Arabidopsis thaliana AKIN beta-1 Q84VQ1, At5g21170
AKIN beta-2 (AKINB2) is a member of SnRK1/SNF1/AMPK family in plants. It is a regulatory subunit of the putative trimeric SNF1-related protein kinase (SnRK) comples which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide derived from Arabidopsis thaliana AKIN beta-2 Q9SCY5
AKING1 is a member of SnRK1/SNF1/AMPK family in plants. It is a regulatory subunit of the putative trimeric SNF1-related protein kinase (SnRK) comples which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide derived from Arabidopsis thaliana AKING1UniProt: Q8LBB2, TAIR: At3g48530
Emanuelle et al. (2015). SnRK1 from Arabidopsis thaliana is an atypical AMPK. Plant J. 2015 Mar 3. doi: 10.1111/tpj.12813.Ramon et al. (2013). The hybrid Four-CBS-Domain KINβγ-subunit functions as the canonical γsubunit of the plant energy sensor SnRK1. Plant j. April 1.
Fructose-1,6 bisphosphate aldolase (ALD) is an enzyme catalazying a key reaction of glycolysis and energy production, converting D-fructose- 1,6-bisphospate into dihydroxyacetone phosphate and D-glyceraldehyde-3-phosphate. This enzyme is present in plant and animal tissues. Plant enzyme is a class I aldolase which does not require a bivalent metal cofactor. It is located to outer mitochondrial membrane.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Glycne max, Oryza sativa, Picea sitchensis, Physcomitrella patens, Pisum sativum, Populus jackii, Spinacia oleracea, Vitis vinifera, Zea mays Species of your interest not listed? Contact us
Immunogen:
overexpressed cytosolic fructose 1,6 bisphosphate aldolase (ALD) based on the sequence from Arabidopsis thaliana Q9LF98, At3g52930
This product can be sold containing ProClin if requested.
Application Details:
1 : 5000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water.
Related products:
AS10 748 | Anti-ADH/ALDH | Alcohol/acetaldehyde dehydrogenase (bacterial/algal), rabbit antibodiesAS13 2731 | Anti-FBA | Fructose-bisphosphate aldolase class 2, rabbit antibodiesAS16 4093 | Anti- FBA | Fructose-bisphosphate aldolase (chloroplastic), rabbit antibodiescollection of antibodies to enzymes involved in carbohydrate metabolism | AgriseraSuperDeal
Molecular Weight:
38 | 38 kDa
Not reactive in:
Synechocystis sp.
Selected references:
Wang et al. (2018). iTRAQ-based quantitative proteomics analysis of an immature high-oleic acid near-isogenic line of rapeseed. Molecular Breeding January 2018, 38:2.Kamies et al. (2017). A Proteomic Approach to Investigate the Drought Response in the Orphan Crop Eragrostis tef. Proteomes. 2017 Nov 15;5(4). pii: E32. doi: 10.3390/proteomes5040032.Foley et al. (2017). A Global View of RNA-Protein Interactions Identifies Post-transcriptional Regulators of Root Hair Cell Fate.Dev Cell. 2017 Apr 24;41(2):204-220.e5. doi: 10.1016/j.devcel.2017.03.018.Parveen et al. (2016). Chickpea Ferritin CaFer1 Participates in Oxidative Stress Response, and Promotes Growth and Development. Sci Rep. 2016 Aug 9;6:31218. doi: 10.1038/srep31218.Yam et al. (2016). Characterization of the Plasmodium Interspersed Repeats (PIR) proteins of Plasmodium chabaudi indicates functional diversity. Sci Rep. 2016 Mar 21;6:23449. doi: 10.1038/srep23449.Dixit (2015). Sulfur alleviates arsenic toxicity by reducing its accumulation and modulating proteome, amino acids and thiol metabolism in rice leaves. Sci Rep. 2015 Nov 10;5:16205. doi: 10.1038/srep16205.Vera-Estrella et al. (2014). Comparative 2D-DIGE analysis of salinity responsive microsomal proteins from leaves of salt-sensitive Arabidopsis thaliana and salt-tolerant Thellungiella salsuginea. J Proteomics. 2014 Jun 2. pii: S1874-3919(14)00288-7. doi: 10.1016/j.jprot.2014.05.018.
α-Amylases are hydrolytic enzymes responsible for the mobilization of the starch into metabolizable sugars. This process provides the energy for the growth of roots and shoots and is crucial during germination of cereal seeds.These enzymes are coded by a multigene family and even thought other amylolytic enzyme participate in the process of starch breakdown, the contribution of α-amylase is the prerequisite for the initiation of this process.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Oryza sativa, Panicum virgatum
Expected Species:
Cereals, Kalanchoe laxiflora Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from known Oryza sativa P17654
AS10 716 | Anti-Ramy 3D | Alpha-amylase isozyme 3D, rabbit antibodiesPlant protein extraction buffer Secondary antibodies | AgriseraSuperDeal
Molecular Weight:
43-48 kDa (depending upon the isoform)
Not reactive in:
Pinus strobus
Selected references:
Ye et al. (2018). Natural variation in the promoter of rice calcineurin B-like protein10 (OsCBL10) affects flooding tolerance during seed germination among rice subspecies. Plant J. 2018 May;94(4):612-625. doi: 10.1111/tpj.13881.
Special application note:
Antibody will detect all alpha amylase isoforms from rice, barley and other cereals.
The mitochondrial AOX (alternative oxidase) of the unicellular green alga Chlamydomonas reinhardtii is encoded by two different genes, the Aox1 and Aox2. The alternative respiratory pathway is comprised of a single homodimeric protein – AOX – and functions as a mechanism to decrease the formation of reactive oxygen species (ROS) produced during respiratory electron transport. Alternative oxidase expression is influenced by different stress stimuli.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Expected Species:
Aspergilus niger, Gonium pectorale, Monoraphidium neglectum, Nannochloropsis gaditana, Ostreococcus lucimarinus, Tetrabaena socialis, Volvox carteri f. nagariensis Species of your interest not listed? Contact us
Immunogen:
whole presumed mature AOX1 protein from from Chlamydomonas reinhardtii UniProt: O65000 fused to GST
AS04 054 | Anti-plant alternative oxidase 1 and 2, polyclonal rabbit antibodiesCollection of antibodies to Chlamydomonas proteinsAlgal protein extraction buffer Secondary antibodies | AgriseraSuperDeal
Molecular Weight:
36 | 36 kDa (Chlamydomonas reainhardii)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Perlaza et al. (2019). The Mars1 kinase confers photoprotection through signaling in the chloroplast unfolded protein response. Elife. 2019 Oct 15;8. pii: e49577. doi: 10.7554/eLife.49577.Kaye et al. (2019). The mitochondrial alternative oxidase from Chlamydomonas reinhardtii enables survival in high light.J Biol Chem. 2019 Jan 25;294(4):1380-1395. doi: 10.1074/jbc.RA118.004667.Zalutskaya et al. (2015). The Chlamydomonas reinhardtii alternative oxidase 1 is regulated by heat stress. Plant Physiol Biochem. 2015 Dec;97:229-34. doi: 10.1016/j.plaphy.2015.10.014.Dang et al. (2014). Combined Increases in Mitochondrial Cooperation and Oxygen Photoreduction Compensate for Deficiency in Cyclic Electron Flow in Chlamydomonas reinhardtii. Plant Cell. 2014 Jul 2. pii: tpc.114.126375.Inman (2013). Characterization of the Alternative Oxidase from the Psychrophilic Green Alga Chlamydomonas sp. UWO241. Thesis.Bohne et al. (2013). Reciprocal Regulation of Protein Synthesis and Carbon Metabolism for Thylakoid Membrane Biogenesis. PLOS, Open Access.
Special application note:
Cellular [compartment marker] of Chlamydomonas reinhardtii mitochondrial inner membrane
Alternative oxidases (AOX) are quinol oxidases located in the inner mitochondrial membrane of plants. They function as terminal oxidases in the alternate electron transport pathway, oxidizing ubiquinone to reduce oxygen to water.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
According to Konert et al. (2015) AOX antibody is recognizing AOX1A and AOX1D.This product can be sold containing ProClin if requested.
Application Details:
1 : 750 (IL), 1 : 1000 for 10-20 µg of mitochondrial protein/lane detection (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 µl of sterile water.
Related products:
AS06 152 Anti-AOX1 | alternative oxidase from Chlamydomonas reinhardtii, rabbit antibodiesAS04 054PRE | AOX1/2 | plant alternative oxidase 1 and 2, pre-immune serumAS04 054S | AOX | AOX positive control/quantitation standardPlant protein extraction bufferSecondary antibodies | AgriseraSuperDeal
Molecular Weight:
36-40 | 36-40 for Arabidopsis thaliana
Not reactive in:
Candidia albicans, Chlamydomonas reinhardtii (use an antibody to algal AOX1, AS06 152)
Selected references:
Makino et al. (2020). Induction of Terminal Oxidases of Electron Transport Chain in Broccoli Heads Under Controlled Atmosphere Storage. Foods, 9 (4) Marchetti et al. (2020). Mitochondrial Pentatricopeptide Repeat Protein, EMB2794, Plays a Pivotal Role in NADH Dehydrogenase Subunit nad2 mRNA Maturation in Arabidopsis Thaliana. Plant Cell Physiol DOI: 10.1093/pcp/pcaa028 Garmash et al. (2020). Altered levels of AOX1a expression result in changes in metabolic pathways in Arabidopsis thaliana plants acclimated to low dose rates of ultraviolet B radiation. Plant Sci. 2020 Feb;291:110332. doi: 10.1016/j.plantsci.2019.110332.Kuang et al. (2019). Quantitative Proteome Analysis Reveals Changes in the Protein Landscape During Grape Berry Development With a Focus on Vacuolar Transport Proteins. Front Plant Sci. 2019 May 15;10:641. doi: 10.3389/fpls.2019.00641. eCollection 2019.Tward et al. (2019). Identification of the alternative oxidase gene and its expression in the copepod Tigriopus californicus. Comp Biochem Physiol B Biochem Mol Biol. 2019 Feb;228:41-50. doi: 10.1016/j.cbpb.2018.11.003.Réthoré et al. (2019). Arabidopsis seedlings display a remarkable resilience under severe mineral starvation using their metabolic plasticity to remain self-sufficient for weeks. Plant J. 2019 Mar 22. doi: 10.1111/tpj.14325.Luévano-Martínez et al. (2019). Mitochondrial alternative oxidase is determinant for growth and sporulation in the early diverging fungus Blastocladiella emersonii. Fungal Biology, Vol 123, Issue 1, 59-65.Córdoba et al. (2019). Different Types of CA Domains Are Present in Complex I from Immature Seeds and Adult Plants in Arabidopsis thaliana. Plant Cell Physiol. 2019 Jan 22. doi: 10.1093/pcp/pcz011.Czobor et al. (2019). Comparison of the response of alternative oxidase and uncoupling proteins to bacterial elicitor induced oxidative burst. PLoS One. 2019 Jan 10;14(1):e0210592. doi: 10.1371/journal.pone.0210592.Hu et al. (2018). OsNDUFA9 encoding a mitochondrial complex I subunit is essential for embryo development and starch synthesis in rice. Plant Cell Rep. 2018 Dec;37(12):1667-1679. doi: 10.1007/s00299-018-2338-x.Borovik and Grabelnych (2018). Mitochondrial alternative cyanide-resistant oxidase is involved in an increase of heat stress tolerance in spring wheat. J Plant Physiol. 2018 Dec;231:310-317. doi: 10.1016/j.jplph.2018.10.007.Umekawa and Ito (2018). Thioredoxin o-mediated reduction of mitochondrial alternative oxidase in the thermogenic skunk cabbage Symplocarpus renifolius. J Biochem. 2018 Oct 5. doi: 10.1093/jb/mvy082.Hu et al. (2018). OsNDUFA9 encoding a mitochondrial complex I subunit is essential for embryo development and starch synthesis in rice.Plant Cell Rep. 2018 Aug 27. doi: 10.1007/s00299-018-2338-x.Zhu et al. (2018). Mitochondrial alternative oxidase-dependent autophagy involved in ethylene-mediated drought tolerance in Solanum lycopersicum. Plant Biotechnol J. 2018 May 4. doi: 10.1111/pbi.12939.Garmash et al. (2017). Expression profiles of genes for mitochondrial respiratory energy-dissipating systems and antioxidant enzymes in wheat leaves during de-etiolation. J Plant Physiol. 2017 Aug;215:110-121. doi: 10.1016/j.jplph.2017.05.023.Vishwakarma et al. (2016). A discrete role for alternative oxidase under hypoxia to increase nitric oxide and drive energy production. Free Radic Biol Med. 2018 Mar 28. pii: S0891-5849(18)30148-5. doi: 10.1016/j.freeradbiomed.2018.03.045.Zhao et al. (2016). Nitrogen deprivation induces cross-tolerance of Poa annua callus to salt stress. Biologia Plantarum 60 (3): 543–554.Solti et al. (2016). Does a voltage-sensitive outer envelope transport mechanism contributes to the chloroplast iron uptake? Planta. 2016 Dec;244(6):1303-1313. Epub 2016 Aug 19.Zhang et al. (2016). A High Temperature-Dependent Mitochondrial Lipase EXTRA GLUME1 Promotes Floral Phenotypic Robustness against Temperature Fluctuation in Rice (Oryza sativa L.). PLoS Genet. 2016 Jul 1;12(7):e1006152. doi: 10.1371/journal.pgen.1006152. eCollection 2016.Meng et al. (2016). Physiological and proteomic responses to salt stress in chloroplasts of diploid and tetraploid black locust (Robinia pseudoacacia L.). Sci Rep. 2016 Mar 15;6:23098. doi: 10.1038/srep23098Pavlovič et al. (2016). Light-induced gradual activation of photosystem II in dark-grown Norway spruce seedlings. Biochim Biophys Acta. 2016 Feb 18. pii: S0005-2728(16)30028-7. doi: 10.1016/j.bbabio.2016.02.009.Konert et al.(2015). Protein phosphatase 2A (PP2A) regulatory subunit B'γ interacts with cytoplasmic ACONITASE 3 and modulates the abundance of AOX1A and AOX1D in Arabidopsis thaliana. New Phytol. 2015 Feb;205(3):1250-63. doi: 10.1111/nph.13097. Epub 2014 Oct 13.
Special application note:
Mitochondrion inner membrane marker. Possibly in the inner surface of the inner mitochondrial membrane.Protocol for a plant mitochondria preparation can be found here. In protein samples which are older than few months AOX enzyme can undergo intensive dimerization. Such preparations should not be used to work with this antibody.
APX plays a key role in plant antioxidant system by reducing hydrogen peroxide to water. Cellular localization includes chloroplast (tAPX and sAPX), cytosol (cAPX) and peroxisome (pAPX).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Brassica rapa subsp. oleifera Stromal APX; Glycine max, Glycine soja L-ascorbate peroxidase T, chloroplastic; Medicago truncatula thylakoid-bound APX; Mesembryanthemum crystallinum, Pisum sativum Chloroplast stromal ascorbate peroxidase 12; Solanum lycopersicum thylakoid-bound APX; Spinacia oleracea stromal APX; Theobroma cacao L-APX T isoform 3; Vitis viniferaSpecies of your interest not listed? Contact us
Immunogen:
BSA-conjugated synthetic peptide derived from Arabidopsis thaliana tAPX (thylakoidal ascorbate peroxidase) UniProt: Q42593-1, TAIR:At1g77490 and sAPX (stromal/mitochondrial ascorbate peroxidase) UniProt: Q42592-1 TAIR: At4g08390 Five out of twelve amino acids are also identical with cAPX1 (At1g07890), cPX2 (At3g09640) and pAPX (At4g35000)
Tokarz et al. (2020). Can Ceylon Leadwort ( Plumbago zeylanica L.) Acclimate to Lead Toxicity?-Studies of Photosynthetic Apparatus Efficiency. Int J Mol Sci. 2020 Mar 9;21(5):1866.doi: 10.3390/ijms21051866. Molnár et al. (2020). Nitro-oxidative Signalling Induced by Chemically Synthetized Zinc Oxide Nanoparticles (ZnO NPs) in Brassica Species. Chemosphere, 251, 126419 Jedelská et al. (2019). Tomato Root Growth Inhibition by Salinity and Cadmium Is Mediated By S-Nitrosative Modifications of ROS Metabolic Enzymes Controlled by S-Nitrosoglutathione Reductase. Biomolecules. 2019 Aug 21;9(9). pii: E393. doi: 10.3390/biom9090393.Szymańska et al. (2019). SNF1-Related Protein Kinases SnRK2.4 and SnRK2.10 Modulate ROS Homeostasis in Plant Response to Salt Stress. Int J Mol Sci. 2019 Jan 2;20(1). pii: E143. doi: 10.3390/ijms20010143.Deng et al. (2019). Integrated proteome analyses of wheat glume and awn reveal central drought response proteins under water deficit conditions. J Plant Physiol. 2019 Jan;232:270-283. doi: 10.1016/j.jplph.2018.11.011.Balfagón et al. (2018). Involvement of ascorbate peroxidase and heat shock proteins on citrus tolerance to combined conditions of drought and high temperatures. Plant Physiol Biochem. 2018Cunha et al. (2016). Salinity and osmotic stress trigger different antioxidant responses related to cytosolic ascorbate peroxidase knockdown in rice roots. Environmental and Experimental Botany, Volume 131, November 2016, Pages 58-67.Ko et al. (2016). Constitutive expression of a fungus-inducible carboxylesterase improves disease resistance in transgenic pepper plants. Planta. 2016 Aug; 244(2):379-92. doi: 10.1007/s00425-016-2514-6. Epub 2016 Apr 13.Yin et al. (2016). Comprehensive Mitochondrial Metabolic Shift during the Critical Node of Seed Ageing in Rice. PLoS One. 2016 Apr 28;11(4):e0148013. doi: 10.1371/journal.pone.0148013. eCollection 2016.Vuleta et al. (2016). Adaptive flexibility of enzymatic antioxidants SOD, APX and CAT to high light stress: The clonal perennial monocot Iris pumila as a study case. Plant Physiol Biochem. 2016 Mar;100:166-73. doi: 10.1016/j.plaphy.2016.01.011. Epub 2016 Jan 19Hattab et al. (2015). Characterisation of lead-induced stress molecular biomarkers in Medicago sativa plants. Environm. Exp. Botany. Volume 123, March 2016, Pages 1–12.Parys et al. (2014). Metabolic Responses to Lead of Metallicolous and Nonmetallicolous Populations of Armeria maritima. Arch Environ Contam Toxicol. 2014 Jul 29.Feifei et al. (2014). Comparison of Leaf Proteomes of Cassava (Manihot esculenta Crantz) Cultivar NZ199 Diploid and Autotetraploid Genotypes. PLoS One. 2014 Apr 11;9(4):e85991. doi: 10.1371/journal.pone.0085991. eCollection 2014.Sobrino-Plata et al. (2014). Glutathione is a key antioxidant metabolite to cope with mercury and cadmium stress. Plant Soil, DOI 10.1007/s11104-013-2006-4.
APX plays a key role in plant antioxidant system by reducing hydrogen peroxide to water.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Expected Species:
Coccomyxa subellipsoidea C-169, Micromonas pusilla (strain CCMP1545), Nannochloropsis gaditana, Ostreococcus lucimarinus (strain CCE9901), Ulva fasciata, Volvox carteri Species of your interest not listed? Contact us
The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. It is a small GTPase that undergoes a GDP/GTP nucleotide exchange cycle and it is an important regulator of cellular trafficking.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
References describing immunolocalization (IF) and (IG) studies:Pimpl et al (2000). In Situ Localization and in Vitro Induction of Plant COPI-Coated Vesicles. Plant Cell. 2000 Nov;12(11):2219-36.Ritzenthaler et al. (2002). Reevaluation of the Effects of Brefeldin A on Plant Cells Using Tobacco Bright Yellow 2 Cells Expressing Golgi-Targeted Green Fluorescent Protein and COPI Antisera. Plant Cell. 2002 Jan;14(1):237-61.
Application Details:
1 : 1000 (IF). 1 : 100 (IG), 1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 µl of sterile water.
Related products:
AS08 327 | Anti-Sec21 (gamma subunit, COP vesicles)(Golgi marker in immunolocalization and COP1 marker in western blot), rabbit antibodiesAS08 325PRE | Arf1 | ADP-ribosylation factor 1, pre-immune serumrecommended secondary antibodyPlant and algal protein extraction buffer Secondary antibodies | AgriseraSuperDeal
Molecular Weight:
21 kDa (Arabidopsis thaliana)
Not reactive in:
Microsporidia sp.
Selected references:
Hurny et al. (2020). SYNERGISTIC ON AUXIN AND CYTOKININ 1 Positively Regulates Growth and Attenuates Soil Pathogen Resistance. Nat Commun. 2020 May 1;11(1):2170. doi: 10.1038/s41467-020-15895-5. (immunolocalization)Kuang et al. (2019). Quantitative Proteome Analysis Reveals Changes in the Protein Landscape During Grape Berry Development With a Focus on Vacuolar Transport Proteins. Front Plant Sci. 2019 May 15;10:641. doi: 10.3389/fpls.2019.00641. eCollection 2019.Singh et al. (2018). A single class of ARF GTPase activated by several pathway-specific ARF-GEFs regulates essential membrane traffic in Arabidopsis. PLoS Genet. 2018 Nov 15;14(11):e1007795. doi: 10.1371/journal.pgen.1007795.Gonzaga Heredia-Martinez et al. (2018). Chloroplast damage induced by the inhibition of fatty acid synthesis triggers autophagy in Chlamydomonas. Plant Physiol, Sept. 2018.Lynch et al. (2017). Multifaceted plant responses to circumvent Phe hyperaccumulation by downregulation of flux through the shikimate pathway and by vacuolar Phe sequestration. Plant J. 2017 Dec;92(5):939-950. doi: 10.1111/tpj.13730.Vincent et al. (2017). A genome-scale analysis of mRNAs targeting to plant mitochondria: upstream AUGs in 5' untranslated regions reduce mitochondrial association. Plant J. 2017 Dec;92(6):1132-1142. doi: 10.1111/tpj.13749.Ma et al. (2016). Phosphatidylserine Synthase Controls Cell Elongation Especially in the Uppermost Internode in Rice by Regulation of Exocytosis. PLoS One. 2016 Apr 7;11(4):e0153119. doi: 10.1371/journal.pone.0153119. eCollection 2016.Yüzbaşıoğlu et al. (2016). Functional specialization of Arf paralogs in nodules of model legume, Medicago truncatula. Plant Growth Regul. DOI: 10.1007/s10725-016-0227-2.Marais et al. (2015). The Qb-SNARE Memb11 interacts specifically with Arf1 in the Golgi apparatus of Arabidopsis thaliana. J Exp Bot. 2015 Jul 24. pii: erv373.Wang et al. (2015). UDP-D-galactose synthesis by UDP-glucose 4-epimerase 4 is required for organization of the trans-Golgi network/early endosome in Arabidopsis thaliana root epidermal cells. J. Plant Res. 2015 May 27. (immunogold application)
Special application note:
Cellular [compartment marker] of Golgi in immunolocalization and COP1 in western blot
ATG12b (Autophagy-related protein 12b) is an Ubiquitin-like protein involved in cytoplasm to vacuole transport (Cvt) and autophagy vesicles formation. Alternative names: Ubiquitin-like protein ATG12B, APG12-like protein b, AtAPG12b.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Immunogen:
Recombinant, full length ATG12b ofArabidopsis thaliana protein sequence UniProt: Q9LVK3-1, TAIR: At3g13970
10.4 | 12 and 50 kDa in wilde type plants and 12 kDa in some autophagy deficient mutants
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Chung et al. (2010). ATG8 lipidation and ATG8-mediated autophagy in Arabidopsis require ATG12 expressed from the differentially controlled ATG12A AND ATG12B loci. Plant J. 2010 May;62(3):483-93. doi: 10.1111/j.1365-313X.2010.04166.x.
ATG13a (Autophagy-related protein 13a) is involved in autophagy in a nutritional condition dependent manner. The ATG1-ATG13 protein kinase complex regulates downstream events required for autophagosome enclosure and/or vacuolar delivery. Alternative names: AtAPG13a.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Noccaea caerulescens Species of your interest not listed? Contact us
Immunogen:
Full length, recombinant ATG13a of Arabidopsis thaliana protein sequence UniProt: Q9SCK0, TAIR: At3g49590 with N-terminal HIS-tag.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Suttangkakul et al. (2011). The ATG1/ATG13 protein kinase complex is both a regulator and a target of autophagic recycling in Arabidopsis. Plant Cell. 2011 Oct;23(10):3761-79. doi: 10.1105/tpc.111.090993.
ATG16 (Autophagy-related protein 16) may play a role in autophagy process.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Immunogen:
N-terminal end of of ATG16 of Arabidopsis thaliana with the PB1 and ZZ domain, UniProt: Q6NNP0-1, TAIR: At5g50230
ATG1a (Serine/threonine-protein kinase ATG1a) is a serine/threonine protein kinase involved in autophagy in a nutritional condition-dependent manner. The ATG1-ATG13 protein kinase complex regulates downstream events required for autophagosome enclosure and/or vacuolar delivery. Becomes a target of autophagy under nutrient starvation and connects autophagy to plant nutritional status. Alternative names: Autophagy-related protein 1a, AtAPG1a.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Noccaea caerulescens Species of your interest not listed? Contact us
Immunogen:
Full length, N-terminally HIS-tagged ATG1a of Arabidopsis thaliana protein sequence UniProt: Q94C95-1, TAIR: At3g61960, overexpressed in E.coli and injected as a gel piece
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Suttangkakul et al. (2011). The ATG1/ATG13 protein kinase complex is both a regulator and a target of autophagic recycling in Arabidopsis. Plant Cell. 2011 Oct;23(10):3761-79. doi: 10.1105/tpc.111.090993.
ATG3 (Autophagy-related protein 3) is a E2 conjugating enzyme responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8. This step is required for the membrane association of ATG8. Alternative names: Autophagy-related E2-like conjugation enzyme ATG3, AtAPG3, Protein autophagy 3.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica campestris, Eutrema salsugineum, Helianthus annuus, Nicotiana tabacum, Noccaea caerulescens, Populus trichocarpa, Salvia splendens, Sisymbrium irio, Solanum tuberosum Species of your interest not listed? Contact us
Immunogen:
Full length recombinant ATG3 of Arabidopsis thaliana protein sequence UniProt: Q0WWQ1-1, TAIR: At5g61500, overexpressed in E.coli
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Phillips et al. (2008). The ATG12-conjugating enzyme ATG10 Is essential for autophagic vesicle formation in Arabidopsis thaliana. Genetics. 2008 Mar;178(3):1339-53. doi: 10.1534/genetics.107.086199.
ATG4 (Autophagy protein 4) is a protein with cyteine-type endopeptidase activity involved in autophagy process.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Expected Species:
Coccomyxa subellipsoidea C-169, Tetraselmis sp. GSL018, Volvox carteri Species of your interest not listed? Contact us
Immunogen:
recombiant ATG4 of Chlamydomonas reinhardtii, Gene ID Cre12.g510100
Technical details how to work with this antibody are provided here: Pérez-Pérez et al. (2016). Control of Autophagy in Chlamydomonas Is Mediated through Redox-Dependent Inactivation of the ATG4 Protease. Plant Physiol. 2016 Dec;172(4):2219-2234.
Chen et al. (2016). The role of nitric oxide signalling in response to salt stress in Chlamydomonas reinhardtii. Planta. 2016 Sep;244(3):651-69. doi: 10.1007/s00425-016-2528-0. Epub 2016 Apr 26.Pérez-Pérez et al. (2016). Control of Autophagy in Chlamydomonas Is Mediated through Redox-Dependent Inactivation of the ATG4 Protease. Plant Physiol. 2016 Dec;172(4):2219-2234.
Special application note:
This antibody is recognizing 25 ng of recombinant CrATG4This product can be sold containing ProClin if requested.
ATG5 (Autophagy protein 5) is a protein required for autophagy. Involved in a negative feedback loop that modulates NPR1-dependent salicylic acid (SA) signaling and limits senescence and immunity-related programmed cell death (PCD) in plants and a complete proteolysis of chloroplast stroma proteins in senescent leaves, in the degradation of damaged peroxisomes. Alternative names: Protein autophagy 5, AtAPG5.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Immunogen:
Full length, recombinant ATG5 of Arabidopsis thaliana protein sequence UniProt: Q9FFI2-1, TAIR: At5g17290, overexpressed in E.coli
38.5 | 50 kDa (in wilde type plants) and ca. 38 kDa in autophagy defective mutants
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Thompson et al. (2005). Autophagic nutrient recycling in Arabidopsis directed by the ATG8 and ATG12 conjugation pathways. Plant Physiol. 2005 Aug;138(4):2097-110.
ATG5 (Autophagy-related protein 5) the protein forms a conjugate with ATG12 with an essential role in plant nutrient recycling. It is involved in complete proteolysis of chloroplast stroma proteins in senescent leaves and degradation of damaged peroxisomes.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Recombinant ATG5 of Arabidopsis thaliana
Immunogen:
Recombinant ATG5 of Arabidopsis thaliana, UniProt:Q9FFI2, TAIR:AT5G17290
AS14 2811 | Anti-ATG8A | Autophagy-related protein 8a, rabbit antibodiesAS12 1854 | Anti-NPR1 | Nonexpresser of PR genes 1 (Arabidopsis thaliana), rabbit antibodiesAS16 4108 | Anti-NPR1 | Nonexpresser of PR genes 1 (other species)Collection of antibodies to plant proteasomePlant and algal protein extraction bufferSecondary antibodies | AgriseraSuperDeal
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Young et al. (2019). A facile forward-genetic screen for Arabidopsis autophagy mutants reveals twenty-one loss-of-function mutations disrupting six ATG genes. Autophagy. 2019 Jun;15(6):941-959. doi: 10.1080/15548627.2019.1569915.
Special application note:
This antibody is so far recognizing recombinant ATG5 from Arabidopsis thaliana and reactivity on endogenous protein needs to be confrimed. It does not react with 6xHis-ATG7.This product can be sold containing ProClin if requested.
ATG7 (Autophagy-related protein 7) is an E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 and ATG8 for its conjugation with phosphatidylethanolamine. Acts in the senescence process, degradation of damaged peroxisomes and non-selective degradation of chlorophylls and photosynthetic proteins during stress-induced leaf yellowing.Alternative names: Ubiquitin-like modifier-activating enzyme atg7, ATG12-activating enzyme E1 atg7, AtAPG7, Protein PEROXISOME UNUSUAL POSITIONING 4.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica rapa, Capsella rubella , Citrus clementina, Noccaea caerulescens Species of your interest not listed? Contact us
Immunogen:
Recombinant ATG7 of Arabidopsis thaliana, UniProt: Q94CD5-1, TAIR: At5g45900, overexpressed in E.coli, collected in inclusion bodies, which were solubilized before immunization
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Doelling et al. (2002). The APG8/12-activating enzyme APG7 is required for proper nutrient recycling and senescence in Arabidopsis thaliana. J Biol Chem. 2002 Sep 6;277(36):33105-14.
Special application note:
Protein name was changed from APG7 to ATG7 after Doelling paper was published.
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Actinidia chinensis var. chinensis, Cajanus cajan, Capsicum annuum, Corchorus olitorius, Cucumis melo, Glycine max, Glycine soja, Gossypium arboreum, Juglans regia, Malus domestica, Nelumbo nucifera, Nicotiana tabacum, Nicotiana benthamiana, Nicotiana sylvestris, Noccaea caerulescens, Prunus yedoensis var. nudiflora, Solanum chacoense, Vigna radiata Species of your interest not listed? Contact us
Immunogen:
Recombinant Arabidopsis thaliana ATG7 UniProt Q94CD5, TAIR AT5G45900
ATG8 (Autophagy-related protein 8) is involved in degradation and recycling of intracellular components in a process of autophagy. ATG8 is a molecular autophagy marker in Chlamydomonas reinhardtii (Pérez-Pérez et al. 2010, Plant Physiol. 152: 1874-88).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Aponogeton madagascariensis, Chlamydononas reinhardtii, Populus trichocarpa, Solanum lycopersicum
Expected Species:
Brassica napus, Micromonas sp., Physcomitrella patens, Pinus sitchensis, Solanum tuberosum, Volvox carteri Species of your interest not listed? Contact us
Immunogen:
Fragment of recombinant ATG8 from Chlamydomonas reinhardtii, UniProt: A8JB85, conserved from 70-80 % in following ATG protein from Arabidopsis thaliana: ATG8a UniProt: Q8LEM4 ATG8B UniProt: Q9XEB5 ATG8c UniProt: Q8S927 ATG8d UniProt: Q9SL04, ATG8e UniProt: Q8S926 ATG8f UniProt: Q8VYK7 and conserved below 70 % in: ATG8g UniProt: Q9LZZ9 ATG8h Uniprot: Q8S925
For Arabidopsis thaliana the signal obtained using ATG8 antibodies is cleaner in case of roots compare to leaf material. For best results please follow extraction protocol described in Álvarez et al. (2012). ATG8 signal corresponds to the two bands of 17 kDa.Preparation of a cell extract from Arabidopsis thaliana:A. Plants were first subjected to autophagy activating conditions: nutrient (nitrogen or carbon) limitation or oxidative stress in order to activate this degradative process.B. Total protein extracts can be obtained as described by Álvarez. Leaves are grinded in liquid nitrogen with a minimal volume of extraction buffer (100 mM Tris-HCl pH 8, 400 mM sucrose, 1 mM EDTA, 0.1 mM phenylmethylsulfonyl fluoride (PMSF), 10 mg/ml sodium deoxycholate, 10 µg/ml of leupeptin, 10 µg/ml of pepstatin A, 4% (v/v) protease inhibitor cocktail from Roche).C. Cell debris is removed by centrifuging at 500 g for 10 min at 4°C.Important note:It is recommendable to use bigger gels in order to get a better resolution of ATG8 bands. Midi-protean gels are better than mini-gels. There are 9 ATG8 isoforms and this antibody will likely recognizes all of them.For immunolocalization protocol, please inquire.
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Kazibwe et al. (2020). TOR mediates the autophagy response to altered nucleotide homeostasis in a ribonuclease mutant. J Exp Bot. 2020 Sep 9;eraa410.doi: 10.1093/jxb/eraa410.Upadhyaya and Jagadeeshwar Rao (2019). Reciprocal regulation of photosynthesis and mitochondrial respiration by TOR kinase in Chlamydomonas reinhardtii. Plant Direct Volume 3, Issue 11.Shull et al. (2019). Anatase TiO2 nanoparticles induce autophagy and chloroplast degradation in thale cress (Arabidopsis thaliana). Environ Sci Technol. 2019 Jul 29. doi: 10.1021/acs.est.9b01648. Wojciechowska et al. (2018). Autophagy counteracts instantaneous cell death during seasonal senescence of the fine roots and leaves in Populus trichocarpa. BMC Plant Biol. 2018 Oct 29;18(1):260. doi: 10.1186/s12870-018-1439-6. (immunolocalization)Chen et al. (2016). The role of nitric oxide signalling in response to salt stress in Chlamydomonas reinhardtii. Planta. 2016 Sep;244(3):651-69. doi: 10.1007/s00425-016-2528-0. Epub 2016 Apr 26.Gorovits et al. (2016). Tomato yellow leaf curl virus confronts host degradation by sheltering in small/midsized protein aggregates. Virus Res. 2016 Feb 2;213:304-13. doi: 10.1016/j.virusres.2015.11.020. Epub 2015 Dec 1
Special application note:
This product can be sold containing ProClin if requested.This antibody is recognizing 1 ng of recombinant CrATG8
ATG9 (Autophagy-related protein 9) is required for autophagy that plays an essential role in plant nutrient recycling. Alternative name: AtAPG9.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Plant ATG9 is most likely N-glycosylated, and glycosylated membrane proteins can migrate as more than one form in SDS-PAGE (smear); the protein most likely forms functional dimers which could be detected also after western blotting. Usage of microsomal fraction is highly recommended.
ATG9 (Autophagy-related protein 9) is required for autophagy that plays an essential role in plant nutrient recycling. Alternative name: AtAPG9.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica campestris, Brassica rapa subsp. pekinensis, Capsella rubella, Eutrema salsugineum Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from Arabidopsis thaliana ATG9 protein sequence, N-terminal part, UniProt: Q8RUS5, TAIR: AT2G31260
ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. AtpA is the largest subunit of the membrane-extrinsic ATP synthase subcomplex.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Dicots and (including Lilium superbum) chloroplast AtpA; may cross-react with mitochondrial AtpA; Algae, Nannochloropsis gaditana, CyanobacteriaSpecies of your interest not listed? Contact us
Immunogen:
Recombinant maize chloroplast AtpA P05022
Applications:
Immunogold (IG), Immunoprecipitation (IP), Western blot (WB)
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Pao et al. (2018). Lamelloplasts and minichloroplasts in Begoniaceae: iridescence and photosynthetic functioning. J Plant Res. 2018 Mar 2. doi: 10.1007/s10265-018-1020-2. (ImmunoGold)Zhang et al. (2017). Nitric oxide induces monosaccharide accumulation through enzyme S-nitrosylation. Plant Cell Environ. 2017 Sep;40(9):1834-1848. doi: 10.1111/pce.12989.Jeon et al. (2017). Functional characterization of chloroplast-targeted RbgA GTPase in higher plants. Plant Mol Biol. 2017 Nov;95(4-5):463-479. doi: 10.1007/s11103-017-0664-y.Murcha et al. (2016). Plant specific Preprotein and Amino Acid Transporter proteins are required for tRNA import into mitochondria. Plant Physiol. 2016 Oct 27. pii: pp.01519.2016.Camejo et al. (2015). Proteomic identification of mitochondrial carbonylated proteins in two maturation stages of pepper fruits. Proteomics. 2015 Aug;15(15):2634-42. doi: 10.1002/pmic.201400370.Yang et al. (2015). Purification and biochemical characterization of the ATP synthase from Heliobacterium modesticaldum. Protein Expr Purif. 2015 May 12. pii: S1046-5928(15)00111-4. doi: 10.1016/j.pep.2015.05.006.
Special application note:
Sequence of the protein used for eliciting this antibody is also conserved in Arabidopsis thaliana AtpA P56757
AAA2 domain is present in ATP binding proteins involved in stress response and being members of clpA/clpB family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Acinetobacter baumannii, Algae, Brassica napus, Cannabis sativa, Clostridium sp., Cyanobacteria, E.coli K-12, Galdieria sulphuraria, Manihot esculenta, Nicotiana plumbaginifolia, Saccharomyces cerevisiae, Salmonella typhimurium, Trichodesmium erythraeum, Triticum aestivum, Vitis vinifera, Zosteria marina, Yrsinia sp. Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from available plant, algal (chloroplastic and mitochondrial) and bacterial sequences of beta subunits of F-type ATP synthases, including Arabidopsis thaliana chloroplastic ATP synthase subunit beta UniProt: P19366, TAIR: AtCg00480 and Arabidopsis thaliana mitochondrial ATP synthase subunit beta-1, UniProt: P83483, TAIR: At5g08670 as well as Chlamydomonas reinhardtii, UniProt: P06541 and A8IQU3
Applications:
Blue Native-PAGE (BN-PAGE), Immunofluorescence (IF), Western blot (WB)
Blue Native gel electrophoresis (BN-PAGE) has been performed on samples solubilized with digitonin (4:1) and loaded at 100 µg/well. Gel thickness was 2 mm with 4.5-16 % gradient.Antibody is recognizing mitochondrial form of AtpB Subota el. al (2011).This antibody can be used as a loading control for bacteria, Bacillus cereus.
Picariello et al. (2020). TIM, a Targeted Insertional Mutagenesis Method Utilizing CRISPR/Cas9 in Chlamydomonas Reinhardtii. PLoS One. 2020 May 13;15(5):e0232594. doi: 10.1371/journal.pone.0232594.Pattanaik et al. (2020). Introduction of a green algal squalene synthase enhances squalene accumulation in a strain of Synechocystis sp. PCC 6803. Metabolic Engineering Communications,Volume 10, June 2020, e00125Mares et al. (2020). Hydrosoluble phylloplane components of Theobroma cacao modulate the metabolism of Moniliophthora perniciosa spores during germination.Fungal Biol. 2020 Jan;124(1):73-81. doi: 10.1016/j.funbio.2019.11.008.Gabilly et al. (2019). Regulation of photoprotection gene expression in Chlamydomonas by a putative E3 ubiquitin ligase complex and a homolog of CONSTANS. Proc Natl Acad Sci U S A. 2019 Aug 12. pii: 201821689. doi: 10.1073/pnas.1821689116.Vojta and Fulgosi (2019). Topology of TROL protein in thylakoid membranes of Arabidopsis thaliana. Physiol Plant. 2019 Jan 20. doi: 10.1111/ppl.12927.Roth et al. (2019). Regulation of Oxygenic Photosynthesis during Trophic Transitions in the Green Alga Chromochloris zofingiensis. Plant Cell. 2019 Feb 20. pii: tpc.00742.2018. doi: 10.1105/tpc.18.00742.Aihara et al. (2019). Algal photoprotection is regulated by the E3 ligase CUL4-DDB1DET1. Nat Plants. 2019 Jan;5(1):34-40. doi: 10.1038/s41477-018-0332-5.Kong et al. (2018) Interorganelle Communication: Peroxisomal MALATE DEHYDROGENASE2 Connects Lipid Catabolism to Photosynthesis through Redox Coupling in Chlamydomonas. Plant Cell. 2018 Aug;30(8):1824-1847. doi: 10.1105/tpc.18.00361Schöttler et al. (2017). The plastid-encoded PsaI subunit stabilizes photosystem I during leaf senescence in tobacco. J Exp Bot. 2017 Feb 1;68(5):1137-1155. doi: 10.1093/jxb/erx009.Jespersen et al. (2017). Metabolic Effects of Acibenzolar-S-Methyl for Improving Heat or Drought Stress in Creeping Bentgrass. Front Plant Sci. 2017 Jul 11;8:1224. doi: 10.3389/fpls.2017.01224. eCollection 2017. (western blot, Agostis stolonifera cv. ‘Penncross’)Fristedt et al. (2015). The thylakoid membrane protein CGL160 supports CF1CF0 ATP synthase accumulation in Arabidopsis thaliana. PLoS One. 2015 Apr 2;10(4):e0121658. doi: 10.1371/journal.pone.0121658. Rurek et al. (2015). Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, organellar translation and ultrastructure. Biochim Biophys Acta. 2015 Jan 21. pii: S0005-2728(15)00016-X. doi: 10.1016/j.bbabio.2015.01.005.Eom et al. (2014). Bacillus subtilis HJ18-4 from Traditional Fermented Soybean Food Inhibits Bacillus cereus Growth and Toxin-Related Genes. J Food Sci. 2014 Nov;79(11):M2279-87. doi: 10.1111/1750-3841.12569. Epub 2014 Oct 30.Lintala et al. (2013). Arabidopsis tic62 trol mutant lacking thylakoid bound ferredoxin-NADP+ oxidoreductase shows distinct metabolic phenotype. Mol Plant Sep 16.Teng et al. (2013). Mitochondrial Genes of Dinoflagellates Are Transcribed by a Nuclear-Encoded Single-Subunit RNA Polymerase. PLOS ONE, June 2013. (immuofluorescence)Rasala et al. (2013). Expanding the spectral palette of fluorescent proteins for the green microalga Chlamydomonas reinhardtii. Plant J. March 23.Heinnickel et al. (2013). Novel thylakoid membrane greencut protein cpld38 impacts accumulation of the cytochrome b6f complex and associated regulatory processes. J. Biol. Chem. Jan 9.
Special application note:
The anti-AtpB antibody will detect the mitochondrial form of the F1 ATP synthase subcomplex, as well as the chloroplastic CF1 Atp Synthase, and most known bacterial F-type Atp Synthases. Peptide used for antibody production is located in a beta sheet, which is partly exposed near the surface of the AtpB protein.Anti-AtpB antibody was used as a loading control in Chlamydomonas reinhardtii and Synechocystis sp. PCC6803.This product can be sold containing proclin if requested
ATP synthase produces ATP from ADP in the presence of a proton gradient across the membrane. F-type ATPases have two components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex. Alternative name of gamma subunit is also: F-ATPase gamma subunit.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Apparent molecular weight of subunit gamma (and as general rule most of ATP synthase subunits) is quite different between Chlamydomonas (42 kDa) and higher plants (38 kDa in spinach), see figure in Lemaire et al. (1989).
Storti et al. (2020). The activity of chloroplast NADH dehydrogenase-like complex influences the photosynthetic activity of the moss Physcomitrella patens. doi.org/10.1101/2020.01.29.924597Pralon et al. (2019). Plastoquinone homoeostasis by Arabidopsis proton gradient regulation 6 is essential for photosynthetic efficiency. Commun Biol. 2019 Jun 20;2:220. doi: 10.1038/s42003-019-0477-4.Li et al. (2019). A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nat Genet. 2019 Apr;51(4):627-635. doi: 10.1038/s41588-019-0370-6.Liang et al. (2018). Thylakoid-Bound Polysomes and a Dynamin-Related Protein, FZL, Mediate Critical Stages of the Linear Chloroplast Biogenesis Program in Greening Arabidopsis Cotyledons. Plant Cell. 2018 Jul;30(7):1476-1495. doi: 10.1105/tpc.17.00972. Epub 2018 Jun 7.Storti et al. (2018). Role of cyclic and pseudo-cyclic electron transport in response to dynamic light changes in Physcomitrella patens. Plant Cell Environ. 2018 Nov 29. doi: 10.1111/pce.13493.Schmid et al. (2018). PUMPKIN, the sole Plastid UMP Kinase, Associates with Group II Introns and Alters Their Metabolism. Plant Physiol. 2018 Nov 8. pii: pp.00687.2018. doi: 10.1104/pp.18.00687.Nikkanen et al. (2018). Regulation of chloroplast NADH dehydrogenase-like complex by NADPH-dependent thioredoxin system. CSH, BioRixiv. doi.org/10.1101/261560Nikkanen et al. (2016). Crosstalk between chloroplast thioredoxin systems in regulation of photosynthesis. Plant Cell Environ. 2016 Aug;39(8):1691-705. doi: 10.1111/pce.12718.Naranjo et al. (2015). The chloroplast NADPH thioredoxin reductase C, NTRC, controls non-photochemical quenching of light energy and photosynthetic electron transport in Arabidopsis. Plant Cell Environ. 2015 Oct 17. doi: 10.1111/pce.12652.Dwyer et al. (2012). Antisense reductions in the PsbO protein of photosystem II leads to decreased quantum yield but similar maximal photosynthetic rates. J. Ex. Bot. 63(13):4781-95.
Special application note:
This product can be sold containing ProClin if requested.
This blocking peptide can be used as a control to neutralize AtpC | gamma subunit of ATP synthase before immunolocalization or western blot. Furter details are provided below.ATP synthase produces ATP from ADP in the presence of a proton gradient across the membrane. F-type ATPases have two components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex. Alternative name of gamma subunit is also: F-ATPase gamma subunit.
Product Type:
Antibody
Format:
Liquid
Storage Temp:
Store lyophilized/reconstituted at -20°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
AS08 312 | Anti-AtpC | gamma subunit of ATP synthase, rabbit antibodies | AgriseraSuperDeal
Molecular Weight:
2 kDa
Special application note:
Estimation of the ratio between antibodies and a peptide : In neutralisation assay use 1.07 µl of AtpC peptide for 10 ml of reaction volume. In case of any questions please contact support@agrisera.com
ATP synthase produces ATP from ADP in the presence of a proton gradient across the membrane. F-type ATPases have two components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.Alternative names: ATPase subunit II, ATP synthase F(0) sector subunit b'
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Chlamydomonas reinhardtii
Expected Species:
Algae, Oryza sativa, Sorghum bicolor, Zea mays, Volvox carteri Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated mix of synthetic peptide derived from Arabidopsis thaliana AtpG Q0WMW8, At4g32260 and Chlamydomonas reinhardtii ATP synthase subunit b' A8J785
F-type ATPase (ATP synthase) is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. Multiple copies of the c subunit build up the ring structure (in spinach a 14-mer of ~112 kDa) of the membrane bound Fo-part of the enzyme.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Chlamydomonas reinhardtii
Expected Species:
Algae, Cannabis sativa, Glycine max, Hordeum vulgare, Oryza sativa, Ostreococcus tauri, Physcomitrella patens, Pinus thunbergii, Pisum sativum, Populus alba, Zea mays, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptides derived from AtpH subunit c of Arabidopsis thaliana UniProt: P56760, TAIR: AtCg00140 and Chlamydomonas reinhardtii UniProt: Q37304
Please note that increased incubation at 95ºC (20-30 min) prior to loading is recommended to break the multimeric c-mer structure, detection of partial ring structures (e.g. 5 or 6 subunits) may occur.
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Schulz et al. (2017). Molecular architecture of the N-type ATPase rotor ring from Burkholderia pseudomallei. EMBO Rep. 2017 Apr;18(4):526-535. doi: 10.15252/embr.201643374.
Special application note:
This product can be sold containing ProClin if requested.
AUX1 (Auxin transporter protein 1) is a carrier protein involved in proton-driven auxin influx. Synthesized in developing leaves and transpported to tips. Involved in lateral root formation, trichoblast polarization and root hair elongation. Required for gravitropism and thigmotropism, especially in roots, by modulating responses to auxin, ethylene and cytokinins. Needed for ammonium-mediated root-growth inhibition. Alternative names: AUX1, AUXIN RESISTANT 1, WAV5, WAVY ROOTS 5, PIR1, MAP1, MODIFIER OF ARF7/NPH4 PHENOTYPES 1, Auxin influx carrier protein 1, Polar auxin transport inhibitor-resistant protein 1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana (recombinant AUX1)
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide derived from protein sequence of Arabidopsis thaliana AUX1. UniProt: Q96247, TAIR: AT2G38120
BAK1 (Brassinosteroid insensitive 1-associated receptor kinase 1) controls expression of genes associated with innate immunity in the absence of pathogens or elicitors. Phosphorylated Brl1. Involved in programmed cell death (PCD). Subcellular localization: cell membrane. Alternative names: BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1, BAK1, BRI1-ASSOCIATED RECEPTOR KINASE, RKS10, RECEPTOR KINASES LIKE SERK 10, SERK3, SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE 3, ELG, ELONGATED, ATSERK3, SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE 3, ATBAK1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Solanum lycopersicum
Expected Species:
Thelungiella halophila Species of your interest not listed? Contact us
Extra Information on CE extraction buffer: CE buffer does not need to be made freshly everytime. Aliquots can be kept at -20°C. Na2MoO4 and NaF are phosphatase inhibitors, included to prevent lose phosphorylation form our protein of interest during extraction. EDTA chelates metal ions and thus inhibits many enzymes which need metal ions as co-factors and inhibits the action of proteases. Protease inhibitor coctail is Sigma product number P 9599 which is used in dilution 1: 100.Antibody is reconizing AtBAK1-Myc in total extracts.
Zhang et al. (2019). An important role of L -fucose biosynthesis and protein fucosylation genes in Arabidopsis immunity. New Phytol. 2018 Dec 15. doi: 10.1111/nph.15639.Hu et al. (2018). A group of receptor kinases are essential for CLAVATA signalling to maintain stem cell homeostasis. Nat Plants. 2018 Apr;4(4):205-211. doi: 10.1038/s41477-018-0123-z. (CoIP)Bundy et al. (2016). A mutation in the catalytic subunit of the glycosylphosphatidyl inositol transamidase disrupts growth, fertility and stomata forma formation in Arabidopsis. Plant Physiology. DOI:10.1104/pp.16.00339.Tateda et al. (2014). Salicylic Acid Regulates Arabidopsis Microbial Pattern Receptor Kinase Levels and Signaling.Plant Cell. 2014 Oct 14. pii: tpc.114.131938.
Special application note:
Antibodies bind to AtBAK1-Myc. They do not cross react with AtSOBIR.This product can be sold containing proclin if requested.
BetaCA1 (Beta carbonic anhydrase 1) (chloroplastic) - is a zinc metalloenzyme that interconvert CO2 and HCO3 (-). Alternative names: ARABIDOPSIS THALIANA SALICYLIC ACID-BINDING PROTEIN 3, ATBCA1, ATSABP3, BETA CARBONIC ANHYDRASE 1, CA1, CARBONIC ANHYDRASE 1, SABP3, SALICYLIC ACID-BINDING PROTEIN 3.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Solanum lycopersicum Q5NE20 Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide, derived in the part C-terminus of BetaCA1 of Arabidopsis thaliana, UniProt: P27140, TAIR: At3g01500
Extraction method – Grind 50 mg of leaf tissue in a sterile microcentrifuge tube using a sterile plastic pestle. Add 132μL of Protein Extraction Buffer (1x TE, 1.2 %SDS, 2.7% sucrose, 7.5 μg mL-1 bromophenol blue) to the ground leaf tissue. Vortex the sample and keep on ice for 15 mins. Centrifuge at 14,000 rpm for five minutes using a benchtop centrifuge. Collect the supernatant and in a new sterile 0.5 ml microcentrifuge tube and discard the pellet.This antibody does not recognize betaCA2.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
DiMario et al. (2016). The Cytoplasmic Carbonic Anhydrases βCA2 and βCA4 Are Required for Optimal Plant Growth at Low CO2. Plant Physiol. 2016 May;171(1):280-93. doi: 10.1104/pp.15.01990.
BIN2 (Brasinosteroid insensitive 2) plays an important role in regulating brassinosteroid signalling which is important for plant growth. It is also implied to mediate auxing signalling pathway. Phosphorylation of BZR1 and BZR2/BES2 by BIN2 increases degradation of these proteins by the proteasome.Alternative names:ATSK21, DWARF 12, DWF12, SHAGGY-LIKE KINASE 21, SK21, UCU1, ULTRACURVATA 1
Product Type:
Antibody
Antibody Type:
Polyclonal
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER. BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel. This antibody has so far not worked in IP.
Application Details:
1 : 8000 (ELISA), 1 : 600 (IF), 1 : 2000 (WB)
Purity:
Affinity purified serum in PBS, pH 7.4
Reconstitution:
For reconstitution add 50 µl of sterile water.
Related products:
AS09 615 | Anti-BiP2 | lumenal-binding protein 2, goat antibodiesAS09 614 | Anti-BiP2 | lumenal-binding protein 2, chicken antibodiesAS09 481PRE | BiP | lumenal-binding protein, pre-immune serumantibodies to plant endomembrane system proteins Plant protein extraction bufferSecondary antibodies | AgriseraSuperDeal
Molecular Weight:
73.5 | 80 kDa
Not reactive in:
Ostreococcus tauri
Selected references:
Hurny et al. (2020). SYNERGISTIC ON AUXIN AND CYTOKININ 1 Positively Regulates Growth and Attenuates Soil Pathogen Resistance. Nat Commun. 2020 May 1;11(1):2170. doi: 10.1038/s41467-020-15895-5. (immunolocalization)Yang et al. (2020). PROTEIN PHOSPHATASE 95 Regulates Phosphate Homeostasis by Affecting Phosphate Transporter Trafficking in Rice. Plant Cell. 2020 Jan 9. pii: tpc.00685.2019. doi: 10.1105/tpc.19.00685.Jang et al. (2020). 1Molecules and CellsCrABCA2 Facilitates Triacylglycerol Accumulation in Chlamydomonas reinhardtii under Nitrogen Starvation. Mol Cells. 2020 Jan 31;43(1):48-57. doi: 10.14348/molcells.2019.0262.Mares et al. (2020). Hydrosoluble phylloplane components of Theobroma cacao modulate the metabolism of Moniliophthora perniciosa spores during germination.Fungal Biol. 2020 Jan;124(1):73-81. doi: 10.1016/j.funbio.2019.11.008.Dalmadi et al. (2019). AGO-unbound cytosolic pool of mature miRNAs in plant cells reveals a novel regulatory step at AGO1 loading. Nucleic Acids Res. 2019 Aug 8. pii: gkz690. doi: 10.1093/nar/gkz690.Feng et al. (2019). Analyses of transgenic fibroblast growth factor 21 mature rice seeds. J-STAGe, Online ISSN : 1347-3735.Bastiaan-Net et al. (2018). IgE Cross-Reactivity of Cashew Nut Allergens. Int Arch Allergy Immunol. 2018 Oct 26:1-14. doi: 10.1159/000493100.Wang et al. (2018). Resistance protein Pit interacts with the GEF OsSPK1 to activate OsRac1 and trigger rice immunity. Proc Natl Acad Sci U S A. 2018 Nov 16. pii: 201813058. doi: 10.1073/pnas.1813058115.Pertl-Obermeyer et al. (2018). Dissecting the subcellular membrane proteome reveals enrichment of H+ (co-)transporters and vesicle trafficking proteins in acidic zones of Chara internodal cells. PLoS One. 2018 Aug 29;13(8):e0201480. doi: 10.1371/journal.pone.0201480. Qiao et al. (2018). Two Crinivirus-Conserved Small Proteins, P5 and P9, Are Indispensable for Efficient Lettuce infectious yellows virus Infectivity in Plants. Viruses. 2018 Aug 28;10(9). pii: E459. doi: 10.3390/v10090459.Mares et al. (2017). Proteomic analysis during of spore germination of Moniliophthora perniciosa, the causal agent of witches' broom disease in cacao. BMC Microbiol. 2017 Aug 17;17(1):176. doi: 10.1186/s12866-017-1085-4.Gelová et al. (2017). Antibody-mediated modulation of cytokinins in tobacco: organ-specific changes in cytokinin homeostasis. J Exp Bot. 2017 Dec 23. doi: 10.1093/jxb/erx426.Nagel et al. (2017). Arabidopsis SH3P2 is an ubiquitin-binding protein that functions together with ESCRT-I and the deubiquitylating enzyme AMSH3. Proc Natl Acad Sci U S A. 2017 Aug 7. pii: 201710866. doi: 10.1073/pnas.1710866114.Lomin et al. (2017). Studies of cytokinin receptor–phosphotransmitter interaction provide evidences for the initiation of cytokinin signalling in the endoplasmic reticulum. Functional Plant Biology, CSIRO Publications. (Nicotiana benthamiana, western blot)Zhang et al. (2017). Control of secondary cell wall patterning involves xylan deacetylation by a GDSL esterase. Nat Plants. 2017 Mar 3;3:17017. doi: 10.1038/nplants.2017.17. (Oryza sativa, immunolocalization, western blot)Je et al. (2016). Signaling from maize organ primordia via FASCIATED EAR3 regulates stem cell proliferation and yield traits. Nat Genet. 2016 Jul;48(7):785-91. doi: 10.1038/ng.3567. Epub 2016 May 16.
BRI1 (Protein BRASSINOSTEROID INSENSITIVE 1) is a receptor which binds brassinolide and has a dual specificity kinase activity acting on both serine/threonine- and tyrosine-containing substrates. Involved in a signaling cascade including expression of light- and stress-regulated genes, promotion of cell elongation, normal leaf and chloroplast senescence, and flowering. Alternative names:BRI1, BRASSINOSTEROID INSENSITIVE 1, CBB2, CABBAGE 2, DWF2, DWARF 2, BIN1, BR INSENSITIVE 1, ATBRI1, Brassinosteroid LRR receptor kinase
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized in PBS pH 7.4.
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica rapa Species of your interest not listed? Contact us
Antibody was tested on bri1-1 and bri1-5 mutants. Bri1-1 is a point mutation in the kinase domain that renders the protein non-functional and plants compensate for that by over-accumulating the non-functional receptor. Bri1-5 is a mutant in the extracellular domain and the bri1-5 protein is retained in the ER. The bri1-5 plants contain less protein than the wild type and show an intermediate brassinosteroid deficient phenotype. Also BRI1-5 migrates higher than wild type BRI1 in SDS-PAGE, because it carries ER-type high mannose N-glycans. For IP: 15 µl GFP-trap beads was used for 200 mg plant material to precipitate GFP-tagged protein followed by detection with Co-IPed BRI1 on Western with 1:5000 diluted anti-BRI1 antibody.Protein extraction has to be done efficiently as this step is crucial, recommended material to buffer ratio: 15 µl/µg or less.
Chen et al. (2019). BES1 is activated by EMS1-TPD1-SERK1/2-mediated signaling to control tapetum development in Arabidopsis thaliana. Nat Commun. 2019 Sep 13;10(1):4164. doi: 10.1038/s41467-019-12118-4.Hou et al. (2019). Less Conserved LRRs Is Important for BRI1 Folding. Front Plant Sci. 2019 May 21;10:634. doi: 10.3389/fpls.2019.00634. eCollection 2019.Chen et al. (2019). BZR1 Family Transcription Factors Function Redundantly and Indispensably in BR Signaling but Exhibit BRI1-Independent Function in Regulating Anther Development in Arabidopsis. Mol Plant. 2019 Jun 20. pii: S1674-2052(19)30207-2. doi: 10.1016/j.molp.2019.06.006.
Special application note:
This product can be sold containing proclin if requested.
YFP (Yellow Fluorescent Protein) is a genetic mutant of green fluorescent protein (GFP). YFP has an excitation peak at 514 nm and emission peak at 527 nm.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized in PBS pH 7.4
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
C-YFP tagged proteins from Arabidopsis thaliana
Immunogen:
KLH-conjugated synthetic peptide derived from C-terminal of YFP protein. This peptide is conserved in pGWB541 Vector.
AS11 1776 | anti-N-YFP | N-terminal of YFP, rabbit antibodiesCollection of antibodies to fluorsecent tagsCollection of antibodies to tag proteinsPlant and algal protein extraction buffer Secondary antibodies | AgriseraSuperDeal
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
CAH3 - carbonic anhydrase is a zinc-containing metalloenzyme that catalyzes the reversible interconversion of CO2 and HCO3?. It plays an important role in many physiological functions that involve decarboxylation or carboxylation reactions, including both photosynthesis and respiration.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Expected Species:
Chlamydomonas reinhardtii (only)
Immunogen:
Recombinant carbonic anhydrase of Chlamydomonas reinhardtii A8J4Z8 after cleavage of a fusion protein
Applications:
immunofluorescence (IF), Immunogold (IG), Western blot (WB)
AS11 1737 | Anti-Beta-CA1, beta-CA2 | carbonic anhydrase | mitochondrial | Chlamydomonas, rabbit antibodiesantibodies to other photosynthetic proteinsCollection of antibodies to Chlamydomonas proteinsAlgal protein extraction buffer Secondary antibodies | AgriseraSuperDeal
Molecular Weight:
33.4 | 29 kDa
Not reactive in:
Arabidopsis thaliana, Oryza sativa
Selected references:
Terentyev (2020: The Main Structural and Functional Characteristics of Photosystem-II-Enriched Membranes Isolated From Wild Type and cia3 Mutant Chlamydomonas reinhardtii. Life (Basel). 2020 May 14;10(5):E63. doi: 10.3390/life10050063..Terentyev et al. (2019). Carbonic anhydrase CAH3 supports the activity of photosystem II under increased pH. Biochim Biophys Acta Bioenerg. 2019 Jul 1;1860(7):582-590. doi: 10.1016/j.bbabio.2019.06.003.Correa-Galvis et al. (2016). Photosystem II Subunit PsbS Is Involved in the Induction of LHCSR Protein-dependent Energy Dissipation in Chlamydomonas reinhardtii. J Biol Chem. 2016 Aug 12;291(33):17478-87. doi: 10.1074/jbc.M116.737312.Mitchell et al. (2014). Dynamics of carbon concentrating mechanism induction and protein re-localisation during the dark to light transition in synchronised Chlamydomonas. Plant Physiol. 2014 Aug 8. pii: pp.114.246918.Tirumani et al. (2014). Regulation of CCM genes in Chlamydomonas reinhardtii during conditions of light-dark cycles in synchronous cultures. Plant Mol Biol. 2014 Mar 4.Shutova et al. (2008). The photosystem II-associated Cah3 in Chlamydomonas enhances the O2evolution rate by proton removal. EMBO J. 27:782-791.
Special application note:
This antibody recognizes native and recombinant carbonic anhydrase of Chlamydomonas reinhardtii.
CAH6 (Carbonic anhydrase 6) is a zinc-containing metalloenzymes that catalyze the reversible hydration of CO2. There are three evolutionary not related families of carbonic anhydrases: alpha, beta and gamma. CAH6 belonds to the beta family and initially was localized to chloroplasts Mitra et al. (2004).However, lately localization to flagella has been confirmed Mackinder et al. 2017.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Expected Species:
Gonium pectorale, Volvox carteri Species of your interest not listed? Contact us
Immunogen:
Recombinant, full length CAH6 of Chlamydomonas reinhardtii, excised from the gel, UniProt: Q6S7R9
Lately localization to flagella has been confirmed for CAH6 Mackinder et al. 2017.Extraction method – harvest the cells and add SDS sample buffer.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Mackinder et al. 2017. A Spatial Interactome Reveals the Protein Organization of the Algal CO2-Concentrating Mechanism. Cell. 2017 Sep 21;171(1):133-147.e14. doi: 10.1016/j.cell.2017.08.044.Mitra et al. (2004). Identification of a new chloroplast carbonic anhydrase in Chlamydomonas reinhardtii. Plant Physiol. 2004 May;135(1):173-82.
Ascorbate peroxidase (APX) is the enzyme catalyzing the ascorbate-dependent reduction of hydrogen peroxide. Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in fruits and photosynthetic tissues. AA is found in every compartment of the plant cell including the apoplast.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized in PBS pH 7.4
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Brassica. juncea, Citrus sinensis, Fragaria ananassa, Gossypium hirsutum, Solanum tuberosum, Sorghum bicolor, Pinus pinaster, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from N-terminal of Zea mays cytosolic APX UniProt: Q41772
Applications:
Immunoprecipitation (IP), Immunolocalization (IL), Western blot (WB)
Bègue et al. (2019). CDC48 regulates ascorbate peroxidase in tobacco. J Exp Bot. 2019 Mar 1. pii: erz097. doi: 10.1093/jxb/erz097.Wang et al. (2018). Resistance protein Pit interacts with the GEF OsSPK1 to activate OsRac1 and trigger rice immunity. Proc Natl Acad Sci U S A. 2018 Nov 16. pii: 201813058. doi: 10.1073/pnas.1813058115. Balážová et al. (2018). Zinc oxide nanoparticles phytotoxicity on halophyte from genus Salicornia. Plant Physiol Biochem. 2018 Sep;130:30-42. doi: 10.1016/j.plaphy.2018.06.013.Adhikari et al. (2018). Sulfate improves cadmium tolerance by limiting cadmium accumulation, modulation of sulfur metabolism and antioxidant defense system in maize. Environmental and Experimental Botany Volume 153, September 2018, Pages 143-162.Ferrer et al. (2018). Differential Pb tolerance in metallicolous and non-metallicolous Zygophyllum fabago populations involves the strengthening of the antioxidative pathways. Environ & Exp Botany, Vo. 150, June 2018, Pages 141-151.Aroca et al. (2015). S-sulfhydration: a new post-translational modification in plant systems. Plant Physiology March 2015 pp.00009.2015.Terrile et al. (2014). Nitric oxide-mediated cell death is triggered by chitosan in F. eumartii spores. Pest Manag Sci. 2014 Apr 25. doi: 10.1002/ps.3814.Tsaniklidis et al. (2013). L-Ascorbic acid metabolism in parthenocarpic and seeded cherry tomatoes. Plant Growth Regul,DOI 10.1007/s10725-013-9845-0. (Solanum lycopersicum, immunolocalization)
Catalase is an enzyme found in most living organisms which is catalazying decomposition of hydrogen peroxide to water and oxygen.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii, Scenedesmus sp.
Expected Species:
Coccomyxa subellipsoidea C-169, Nannochloropsis gaditana, Ulva prolifera, Zosteria marina Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide chosen from Chlamydomonas reinhardtii catalase sequence, UniProt: A8J537
Catalase is an enzyme found in most living organisms which is catalazying decomposition of hydrogen peroxide to water and oxygen. In plant cells catalase is found in peroxisomes. This enzyme is involved in photorespiration and symbiotic nitrogen fixation.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Aponogeton madagascariensis, Brassica oleracea, Hordeum vulgare, Lupinus albus, Moniliophthora perniciosa, Musa acuminate, Musa paradisiaca L., Nicotiana bentamina, Nicotiana tabacum, Oryza sativa, Paulownia tomentosa, Plumbago zeylanica, Setaria italica L. P. Beauv, Solanum lycopersicum, Spinacia oleracea, Triticum aestivum, Zea mays, Vitis vinifera
To obtain reactivity with Solanum lycopersicum urea gel needs to be apply. Please, contact us for more details.To decrease background signal this antibody needs to be incubated in PBS-T NOT TBS-T. For reference, check image in application example below.
Tokarz et al. (2020). Can Ceylon Leadwort ( Plumbago zeylanica L.) Acclimate to Lead Toxicity?-Studies of Photosynthetic Apparatus Efficiency. Int J Mol Sci. 2020 Mar 9;21(5):1866.doi: 10.3390/ijms21051866. Boussardon et al. (2020). Tissue-specific Isolation of Arabidopsis/plant Mitochondria - IMTACT (Isolation of Mitochondria Tagged in Specific Cell Types). Plant J. 2020 Feb 14. doi: 10.1111/tpj.14723. Online ahead of print.Rodriguez et al. (2020). Autophagy mediates temporary reprogramming and dedifferentiation in plant somatic cells. bioRxiv doi.org/10.1101/747410Calero-Muñoz et al. (2019). Cadmium induces reactive oxygen species-dependent pexophagy in Arabidopsis leaves. Plant Cell Environ. 2019 Sep;42(9):2696-2714. doi: 10.1111/pce.13597.Mares et al. (2020). Hydrosoluble phylloplane components of Theobroma cacao modulate the metabolism of Moniliophthora perniciosa spores during germination.Fungal Biol. 2020 Jan;124(1):73-81. doi: 10.1016/j.funbio.2019.11.008.Calero-Muñoz et al. (2019). Cadmium induces ROS-dependent pexophagy in Arabidopsis leaves. Plant Cell Environ. 2019 May 31. doi: 10.1111/pce.13597.Szymańska et al. (2019). SNF1-Related Protein Kinases SnRK2.4 and SnRK2.10 Modulate ROS Homeostasis in Plant Response to Salt Stress. Int J Mol Sci. 2019 Jan 2;20(1). pii: E143. doi: 10.3390/ijms20010143.Bastow et al. (2018). Vacuolar Iron Stores Gated by NRAMP3 and NRAMP4 Are the Primary Source of Iron in Germinating Seeds. Plant Physiol. 2018 Jul;177(3):1267-1276. doi: 10.1104/pp.18.00478.Pan et al. (2018). Comparative proteomic investigation of drought responses in foxtail millet. BMC Plant Biol. 2018 Nov 29;18(1):315. doi: 10.1186/s12870-018-1533-9.Su et al. (2018). The Arabidopsis catalase triple mutant reveals important roles of catalases and peroxisome derived signaling in plant development. J Integr Plant Biol. 2018 Mar 25. doi: 10.1111/jipb.12649.Kang et al. (2018). Autophagy-related (ATG) 11, ATG9 and the phosphatidylinositol 3-kinase control ATG2-mediated formation of autophagosomes in Arabidopsis. Plant Cell Rep. 2018 Jan 19. doi: 10.1007/s00299-018-2258-9.Mares et al. (2017). Proteomic analysis during of spore germination of Moniliophthora perniciosa, the causal agent of witches' broom disease in cacao. BMC Microbiol. 2017 Aug 17;17(1):176. doi: 10.1186/s12866-017-1085-4.Sultan et al. (2017). The Reverse Transcriptase/RNA Maturase Protein MatR Is Required for the Splicing of Various Group II Introns in Brassicaceae Mitochondria. Plant Cell. 2016 Nov;28(11):2805-2829.Zhang et al. (2017). Global analysis of protein lysine succinylation profiles in common wheat. BMC Genomics. 2017 Apr 20;18(1):309. doi: 10.1186/s12864-017-3698-2. (Triticum aestivum L., immunoprecipitation)Kneeshaw et al. (2017). Nucleoredoxin guards against oxidative stress by protecting antioxidant enzymes. Proc Natl Acad Sci U S A. 2017 Jul 19. pii: 201703344. doi: 10.1073/pnas.1703344114.Dauphinee et al. (2017). Remodelling of lace plant leaves: antioxidants and ROS are key regulators of programmed cell death. Planta. 2017 Apr 7. doi: 10.1007/s00425-017-2683-y. (Aponogeton madagascariensis)Yin et al. (2016). Comprehensive Mitochondrial Metabolic Shift during the Critical Node of Seed Ageing in Rice. PLoS One. 2016 Apr 28;11(4):e0148013. doi: 10.1371/journal.pone.0148013. eCollection 2016.Lee et al. (2016). Superoxide serves as a putative signal molecule for plant cell division: overexpression of CaRLK1 promotes the plant cell cycle via accumulation of O2 − and decrease in H2O2. Physiol Plantarum. doi:10.1111/ppl.12487
Special application note:
This antibody is recognizing all three isoforms of Arabidopsis thaliana catalase. Catalase-2 is a main isoform expressed in leaf tissue and localized to peroxisomes. This antibody contains 0.1 % ProClin.
CBP20 (Nuclear cap-binding protein subunit 2)is a component of the cap-binding complex (CBC), involved in various processes such as pre-mRNA splicing and RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs). Alternative names: 20 kDa nuclear cap-binding protein, NCBP 20 kDa subunit
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Glycne max, Hordeum vulgare, Lotus corniculatus, Nicotiana tabacum, Oryza sativa, Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Zea mays Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide, derived with Arabidopsis thaliana CBP20 protein Q9xFD1, At5g44200
CBP80 is a component of the cap-binding complex (CBC), which binds co-transcriptionally to the 5' cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing. Alternative names: 80 kDa nuclear cap-binding protein, NCBP 80 kDa subunit, abscisic acid-hypersensitive protein 1, ABA-hypersensitive protein 1, Protein ENSALADA
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
CCA1 (Circadian clocl associated 1) encodes a transcriptional repressor that performs overlapping functions with LHY in a regulatory feedback loop that is closely associated with the circadian oscillator of Arabidopsis. Binds to the evening element in the promoter of TOC1 and represses TOC1 transcription. CCA1 and LHY colocalize in the nucleus and form heterodimers in vivo. CCA1 and LHY function synergistically in regulating circadian rhythms of Arabidopsis.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Immunogen:
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana CCA1 protein sequence, UniProt:P92973, TAIR:AT2G46830
Important note about protein extractionTranscription factors are best isolated by freezing nucelar extract in liquid nitrogen after addition of extraction buffer, followed by thawing directly at 100°C heating block, not at RT. Centrifugation for 5 minutes will remove cell debris. Protein concenration is measure ad rest is frozen in liquid Nitrogen, in aliquots. Frozen samples are thawed at RT and loaded as soon as they are in liquid form, without heating. Such precautions are necessary to take to be able to detect a transcription factor using antibodies.
Application Details:
1: 500 (Co-IP), 1 : 500 (WB)
Purity:
Affinity purified serum in PBS, pH 7.4
Reconstitution:
For reconstitution add 50 µl of sterile water.
Related products:
AS13 2661 | Anti-LHY | Late elongated hypocotyl, rabbit antibodiesAS13 2646 | Anti-TOC1 | TIMING OF CAB EXPRESSION 1, rabbit antibodiesPlant protein extraction buffer Secondary antibodies | AgriseraSuperDeal
Molecular Weight:
67 | 80 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Hung er al. (2018). The Arabidopsis LDL1/2-HDA6 histone modification complex is functionally associated with CCA1/LHY in regulation of circadian clock genes. Nucleic Acids Res. 2018 Aug 14. doi: 10.1093/nar/gky749.
Copper is an essential element for function of chloroplasts. It is a co-factor for superoxide dismutase (SOD) and for plastocyanin. Copper is transported to chloroplasts with a help of Copper Chaperones, which carry out the delivery of copper from transporters to targets. In the last step chaperone binds to a copper dependent enzyme and inserts the copper ions into its active site. The Arabidopsis thaliana gene AtCCS encodes a copper chaperone for SOD. The AtCCS protein was localized to chloroplasts where it may supply copper to the stromal Cu/ZnSOD.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Glycne max, Solanum tuberosum Species of your interest not listed? Contact us
Immunogen:
The antibody was raised to the Arabidopsis thaliana full length CCs protein Q4ZJI5, fused to His-tag. Overexpressed using pET28a vector in Bl21 E. coli (Cd+) and purified using His-column. After purification, the His tag was cleaved using thrombin and then theprotein was purified using Resource-Q column connected to HPLC.
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Speth et al. (2013). RACK1 scaffold proteins influence miRNA abundance in Arabidopsis. Plant J. Aug 13.Abdel-Ghany et al (2005). .AtCCS is a functional homolog of the yeast copper chaperone Ccs1/Lys7. FEBS Lett. 11:2307-12.
Programmed cell death (PCD) is a fundamentally important biological process required to maintain the integrity and homeostasis of multicellular organisms during normal development and as a response to adverse environments. Cdc2 kinase, plays a major role in driving the cell cycle. Synonymes (for Arabidopsis thaliana): CDC2A, CDKA-1
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
collection of antibodies for DNA/RNA metabolism and cell cyclePlant protein extraction buffer Secondary antibodies | AgriseraSuperDeal
Molecular Weight:
34-36 (Zea mays)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Syu et al. (2014). Impacts of size and shape of silver nanoparticles on Arabidopsis plant growth and gene expression. Plant Physiology and Biochemistry 2014. In print.
CesA4 (IRX5) (EC=2.4.1.12) is a catalytic subunit of xylem-specific cellulose synthase enzyme, involved in secondary cell wall biosynthesis - cellulose synthase trerminal complexes. It interacts with CESA7 and CESA8 which is required for a functyional complex and localization in secondary cell wall deposition sites. Expressed in young plants, stems and flowers but not in leaves, roots and shoots. Amounts are increasing as stems mature. Synonymes: Protein IRREGULAR XYLEM 5, IRX5, NWS2.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Betula luminifera, Brassica napus, Capsella rubella, Eutrema salsugineum, Gossypium hirsutum, Nelumbo nucifera, Noccaea caerulescens, Vitis vinifera Species of your interest not listed? Contact us
Otulak-Kozieł et al. (2018). Plant Cell Wall Dynamics in Compatible and Incompatible Potato Response to Infection Caused by Potato Virus Y (PVYNTN). Int J Mol Sci. 2018 Mar 15;19(3). pii: E862. doi: 10.3390/ijms19030862.Tsuchiya et al. (2015). Distribution of XTH, expansin, and secondary-wall-related CesA in floral and fruit abscission zones during fruit development in tomato (Solanum lycopersicum). Front Plant Sci. 2015 May 15;6:323. doi: 10.3389/fpls.2015.00323.
Special application note:
This antibody is detecting both, recombinant and edogenous CesA4 (IRX5) protein.
CesA7 (IRX3) is a catalytic subunit of xylem-specific cellulose synthase, involved in secondary cell wall biosynthesis - cellulose synthase trerminal complexes. It interacts with CESA4 and CESA8 which is required for a functyional complex and localization in secondary cell wall deposition sites. Occurs in secondary cell wall developing tissues such as xylems and interfascicular regions in young plants stems and flowers. Not expressed in leaves, roots and shoots. Synonymes: Protein IRREGULAR XYLEM 3, IRX3, Protein FRAGILE FIBER 5.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Solanum lycopersicum
Expected Species:
Brassica napus, Eucalyptus grandis, Nicotiana tabacum, Populus sp. Species of your interest not listed? Contact us
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Tsuchiya et al. (2015). Distribution of XTH, expansin, and secondary-wall-related CesA in floral and fruit abscission zones during fruit development in tomato (Solanum lycopersicum). Front Plant Sci. 2015 May 15;6:323. doi: 10.3389/fpls.2015.00323.
CesA8 (IRX1) is a catalytic subunit of an enzyme involved in secondary cell wall biosynthesis - cellulose synthase trerminal complexes. It interacts with CESA4 and CESA7 which is required for a functyional complex. The protein is needed for xylem cell wall thickening. Occurs in secondary cell wall developing tissues such as xylems and interfascicular regions. Not expressed in leaves and not found in embryos. Synonymes: Protein IRREGULAR XYLEM 1, IRX1, Protein LEAF WILTING 2, LEW2 .
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Zhang et al. (2016). Golgi-localized STELLO proteins regulate the assembly and trafficking of cellulose synthase complexes in Arabidopsis. Nat Commun. 2016 Jun 9;7:11656. doi: 10.1038/ncomms11656.Tsuchiya et al. (2015). Distribution of XTH, expansin, and secondary-wall-related CesA in floral and fruit abscission zones during fruit development in tomato (Solanum lycopersicum). Front Plant Sci. 2015 May 15;6:323. doi: 10.3389/fpls.2015.00323.
Special application note:
This antibody is detecting both, recombinant and edogenous CesA8 (IRX1) protein.
Fructose-1,6 bisphosphatase (FBPase) (EC=3.1.311) is one of the regulatory enzymes in the sucrose biosynthetic pathway. In non-photosynthetic tissues, it regulates the rate of gluconeogenesis. In photosynthetic tissues, two FBPase isozymes (chloroplastic and cytosolic) play key roles in carbon assimilation and metabolism. In photosynthetic tissues cFBPase (cytosolic fructose 1,6 bisphosphatase) converts triose phosphates from the chloroplast to sucrose during light hours. Alternative name: D-fructose-1,6-bisphosphate 1-phosphohydrolase
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Capsella rubella, Pisum sativum, Ricinus communis, Glycine max, Phaseolus vulgaris, Sesamum indicum, Spinacia oleracea, Populus trichocarpa, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
Overexpressed cytosolic fructose 1,6 bisphosphatase (cFBPase) derived from the sequence from Arabidopsis thaliana cFBPase UniProt: Q9MA79, TAIR: AT1G43670
This antibody does not react with chloroplastic form of FBPase.Will this antibody be good as a cytosolic (non-microsomal control) in Arabidopsis thaliana roots? Although it has never been tested there is every likelihood that cFBPase will be expressed at reasonable levels even in roots. Even though the biosynthetic flux through to Sucrose may not be high as in mesophyll cells, central metabolism will still be active in young roots and the Sucrose etc being supplied externally still needs to be utilised.
Application Details:
1 : 5 000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 µl of sterile water
Related products:
Collection of other antibodies which can be used as markers of cytoplasmPlant protein extraction bufferSecondary antibodies | AgriseraSuperDeal
Molecular Weight:
45 | 37 kDa (Arabidopsis thaliana)
Not reactive in:
Chlamydomonas reinhardtii
Selected references:
Shahbaz and Pilon (2019). Conserved Cu-MicroRNAs in Arabidopsis thaliana Function in Copper Economy under Deficiency. Plants (Basel). 2019 May 29;8(6). pii: E141. doi: 10.3390/plants8060141.Patir-Nebioglu et al. (2019). Pyrophosphate modulates plant stress responses via SUMOylation. Elife. 2019 Feb 20;8. pii: e44213. doi: 10.7554/eLife.44213.Liu et al. (2019). IMPORTIN β4 mediates nuclear import of GRF-INTERACTING FACTORs to control ovule development in Arabidopsis. Plant Physiol. 2019 Jan 18. pii: pp.01135.2018. doi: 10.1104/pp.18.01135.Seguel et al. (2018). PROHIBITIN 3 forms complexes with ISOCHORISMATE SYNTHASE 1 to regulate stress-induced salicylic acid biosynthesis in Arabidopsis. Plant Physiol. Jan 2018. DOI:10.1104/pp.17.00941Lynch et al. (2017). Multifaceted plant responses to circumvent Phe hyperaccumulation by downregulation of flux through the shikimate pathway and by vacuolar Phe sequestration. Plant J. 2017 Dec;92(5):939-950. doi: 10.1111/tpj.13730.Duan et al. (2017). A Lipid-Anchored NAC Transcription Factor Is Translocated into the Nucleus and Activates Glyoxalase I Expression during Drought Stress. Plant Cell. 2017 Jul;29(7):1748-1772. doi: 10.1105/tpc.17.00044. (Nicotiana benthamiana)Steffens et al. (2017). Physical, Functional and Genetic Interactions between the BEACH Domain Protein SPIRRIG and LIP5 and SKD1 and Its Role in Endosomal Trafficking to the Vacuole in Arabidopsis. Front Plant Sci. 2017 Nov 20;8:1969. doi: 10.3389/fpls.2017.01969.Xing et al. (2016). Proteome Profile of Starch Granules Purified from Rice (Oryza sativa) Endosperm. PLoS One. 2016 Dec 19;11(12):e0168467. doi: 10.1371/journal.pone.0168467.LaMontagne et al. (2016). Isolation of Microsomal Membrane Proteins from Arabidopsis thaliana. Curr. Protoc. Plant Biol. 1:217-234. doi: 10.1002/cppb.20020.Ma et al. (2016). Phosphatidylserine Synthase Controls Cell Elongation Especially in the Uppermost Internode in Rice by Regulation of Exocytosis. PLoS One. 2016 Apr 7;11(4):e0153119. doi: 10.1371/journal.pone.0153119. eCollection 2016.de Michele et al. (2016). Free-Flow Electrophoresis of Plasma Membrane Vesicles Enriched by Two-Phase Partitioning Enhances the Quality of the Proteome from Arabidopsis Seedlings. J Proteome Res. 2016 Mar 4;15(3):900-13. doi: 10.1021/acs.jproteome.5b00876. Epub 2016 Feb 4.
Special application note:
Kinetic and allosteric properties of the plant cytosolic FBPase are remarkably similar to the mammalian and yeast FBPase, but differ greatly from those of the chloroplastic FBPase. The antibody could detect FBPase from the human COS-7 cell line transfected with FBP1 expressing vector.This product can be sold containing ProClin if requested.
CGL160 (Conserved in green lineage 160) is a protein encoded in the nucleus and localized in chloroplast. It interacts with components of the CF1 sub-complex. Synonymes: ATCGL160.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Immunogen:
part of Arabidopsis thaliana recombinant CGL160 derived from a following sequence UniProt: O82279, TAIR: At2g31040
AS08 370 | Anti-ATPase | ATP synthase, whole enzyme, rabbit antibodycollection of antibodies to GreenCut proteinsPlant and algal protein extraction buffer Secondary antibodies | AgriseraSuperDeal
Molecular Weight:
38.6 | 34 kDa (without transit peptide)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Galvis et al. (2020). H+ transport by K+ EXCHANGE ANTIPORTER3 promotes photosynthesis and growth in chloroplast ATP synthase mutants. Plant Physiol. pp.01561.2019. doi: 10.1104/pp.19.01561.Fristedt et al. (2015). The Thylakoid Membrane Protein CGL160 Supports CF1CF0 ATP Synthase Accumulation in Arabidopsis thaliana. PLoS One. 2015 Apr 2;10(4):e0121658. doi: 10.1371/journal.pone.0121658.
Chalcone synthase (CHS) is an essential enzyme in flavonoid biosynthesis. Required for the accumulation of purple anthocyanins in leaves and stems. Also involved in the regulation of auxin transport and the modulation of root gravitropism. Alternative names: Naringenin-chalcone synthase, Protein TRANSPARENT TESTA 4.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Cannabis sativa, Brassica sp, Gossypium hirsutum, Nicotiana tabacum, Oryza sativa, Solanum tuberosum, Petroselinum sp., Pisum sativum, Triticum aestivum, Zea mays, Vitis vinifera Species of your interest not listed? Contact us
Plant protein extraction buffer Secondary antibodies | AgriseraSuperDeal
Molecular Weight:
43.1 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Nabbie et al. (2017). Lambda Protein Affects Anthocyanin Production in Arabidopsis thaliana during Drought Stress. Journal of Agricultural Science; Vol. 9, No. 7; 2017 (immunolocalization, western blot)
Clathrin is a protein involved in intracellular trafficking and plays a major role in the formation of coated vesicles. It consists of three clathrin heavy chains and three light chains. Clathrin-coated vesicles (CCV) selectively sort cargo at the cell membrane, trans-Golgi network, and endosomal compartments for multiple membrane traffic pathways.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Amborella trichopoda, Brassica napus, Capsella rubella, Citrus aurantium var. sinensis, Eucalyptus grandis, Glycine max, Chlorella variabilis, Leucaena glauca, Lotus japonicus, Medicago tribuloides, Mimulus guttatus, Musa malaccensis, Oryza sativa, Panicum italicum, Physcomitrella patens, Phaseolus vulgaris, Pisum sativum, Populus balsamifera, Populus trichocarpa, Ricinus communis, Selaginella moellendorffii, Sisymbrium salsugineum, Solanum lycopersicum, Theobroma cacao, Triticum aestivum, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from available plant clathrin heavy chain sequences including Arabidopsis thaliana clathrin heavy chain 1 UniProt: Q0WNJ6, TAIR:At3g11130, clathrin heavy chain 2 UniProt: Q0WLB5,TAIR:At3g08530
Applications:
Immunolocalization (IL), Immunofluorescence (IF), Western blot (WB)
Ranjan et al. (2019). Transient Internalization and Microtubule-Dependent Trafficking of a Ciliary Signaling Receptor from the Plasma Membrane to the Cilium. Curr Biol. 2019 Aug 2. pii: S0960-9822(19)30867-X. doi: 10.1016/j.cub.2019.07.022.Wattelet-Boyer et al. (2016). Enrichment of hydroxylated C24- and C26-acyl- chain sphingolipids mediates PIN2 apical sorting at trans-Golgi network subdomains. Nat Commun. 2016 Sep 29;7:12788. doi: 10.1038/ncomms12788.Derbyshire et al. (2015). Proteomic Analysis of Microtubule Interacting Proteins over the Course of Xylem Tracheary Element Formation in Arabidopsis. Plant Cell. 2015 Oct 2. pii: tpc.15.00314.Grones et al. (2015). Auxin-binding pocket of ABP1 is crucial for its gain-of-function cellular and developmental roles. J Exp Bot. 2015 Apr 28. pii: erv177.McLoughlin et al. (2013). Identification of novel candidate phosphatidic acid binding proteins involved in the salt stress response of Arabidopsis thaliana roots. Biochem J. Jan 17.
ClpB-P is involved in chloroplast biogenesis. Synonymes: ALBINO AND PALE GREEN 6, APG6, AT5G15450, CASEIN LYTIC PROTEINASE B3, CASEIN LYTIC PROTEINASE B-P, CLPB3, CLPB-P, T20K14_60, T20K14.60
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Populus trichocarpa, Ricinus communis, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from ClpB-P of Arabidopsis thaliana Q9LF37, At5g15450
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Han et al. (2015). A nuclear-encoded chloroplast-targeted S1 RNA-binding domain protein affects chloroplast rRNA processing and is crucial for the normal growth of Arabidopsis thaliana. Plant J. 2015 Jul;83(2):277-89. doi: 10.1111/tpj.12889. Epub 2015 Jun 15.
Special application note:
There is a cross-reacting band at ca. 95 kDa - therefore longer electrophoresis time is adviced to obtain good separation.
ClpC is a chloroplastic protein of the Hsp100 family. It is believed to function as a housekeeping enzyme, both in its capacity as an independent molecular chaperone and as the regulatory component of the Clp protease.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Algae (red), Catalpa bungei, Nicotiana tabacum, Ostreococcus sp., Oryza sativa, Populus trichocarpa, Physcomitrella patens, Pisum sativum, Solanum tuberosum, Zea mays Species of your interest not listed? Contact us
Immunogen:
Recombinant ClpC (C-terminal domain overexpressed as fusion with maltose-binding protein) Q55023
Applications:
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western blot (WB)
Jiang et al. (2020). Plastid chaperone HSP90C guides precursor proteins to the SEC translocase for thylakoid transport. J Exp Bot. 2020 Aug 27;eraa399.doi: 10.1093/jxb/eraa399. Lee et al. (2018). Prolines in Transit Peptides Are Crucial for Efficient Preprotein Translocation into Chloroplasts. Plant Physiol. 2018 Jan;176(1):663-677. doi: 10.1104/pp.17.01553. Epub 2017 Nov 20.Hu et al. (2015). Site-specific Nitrosoproteomic Identification of Endogenously S-Nitrosylated Proteins in Arabidopsis. Plant Physiol. 2015 Feb 19. pii: pp.00026.2015.Rosano et al. (2011). Insights into the Clp/HSP100 chaperone system from chloroplasts of Arabidopsis thaliana. J Biol Chem. Aug 26;286(34):29671-80. (Western blot, Arabidopsis thaliana)Karradt et al. (2008) NblA, a Key Protein of Phycobilisome Degradation, Interacts with ClpC, a HSP100 Chaperone Partner of a Cyanobacterial Clp Protease. J Biol Chem 283: 32394-32403.Porankiewicz & Clarke (1997) Induction of the heat shock protein ClpB affects cold acclimation in the cyanobacterium Synechococcs sp. strain PCC7942. J Bacteriol 179:5111-5117.
Special application note:
Anti-ClpC antibodies will also recognized Arabidopsis thaliana isoform ClpC1 (At5g50920) and ClpC2 (At3g48870).
CNX1/2 (calnexin homolog 1/2) is a calcium-binding protein involved in protein folding. It interacts with newly synthesized glycoproteins in the endoplasmic reticulum.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Aliquite upon arrival to avoid repeated freeze-thaw cycles and store -20°C; Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Brassica napus, Coccomyxa suellipsoidea, Hordeum vulgare, Glycine max, Medicago truncatula, Oryza sativa, Petunia inflata, Physcomitrella patens, Picea sitcHensis, Pisum sativum, Populus trichocarpa, Ricinus communis, Stereum hirsutum, Zea mays, Vitis viniferaSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana CNX1 UniProt: P29402 TAIR: AT5G61790, CNX2 UniProt: Q38798, TAIR: AT5G07340. This peptide is NOT present in calreticulins.
CNX1 60.5 kD, processing aa 1-20, mature peptide 58.1 kDCNX2 60.5/61.4 kD, processing aa 1-25, mature peptides 57.6/58.6 kD
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Kramer et al. (2020). N6-methyladenosine and RNA secondary structure affect transcript stability and protein abundance during systemic salt stress in Arabidopsis. Plant Direct . 2020 Jul 24;4(7):e00239.doi: 10.1002/pld3.239.Collins et al. (2020). EPSIN1 Modulates the Plasma Membrane Abundance of FLAGELLIN SENSING2 for Effective Immune Responses . Plant Physiol. 2020 Feb 24. pii: pp.01172.2019. doi: 10.1104/pp.19.01172Butler et al. (2019). Soybean resistance locus Rhg1 confers resistance to multiple cyst nematodes in diverse plant species. Phytopathology. 2019 Aug 12. doi: 10.1094/PHYTO-07-19-0225-R.Howden et al. (2017), Quantitative analysis of the tomato nuclear proteome during Phytophthora capsici infection unveils regulators of immunity. New Phytol. 2017 Jul;215(1):309-322. doi: 10.1111/nph.14540. Foley et al. (2017). A Global View of RNA-Protein Interactions Identifies Post-transcriptional Regulators of Root Hair Cell Fate.Dev Cell. 2017 Apr 24;41(2):204-220.e5. doi: 10.1016/j.devcel.2017.03.018.LaMontagne et al. (2016). Isolation of Microsomal Membrane Proteins from Arabidopsis thaliana. Curr. Protoc. Plant Biol. 1:217-234. doi: 10.1002/cppb.20020.
CO (CONSTANS) is a putative transcription factor which is involved in the long day flowering pathway and has been implied to control mediation between circadian clock and flowering. It regulates flowering time by interactions with SOC1 (suppressor of CO 1), TF1 (terminal flower 1), and FT (flowering locus T). Furthermore it is involved in proline and ethylene biosynthesis.
Product Type:
Antibody
Antibody Type:
Polyclonal
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Goat
Species Reactivity:
Arabidopsis thaliana (recombianant CO)
Expected Species:
Camelina sativa, Chrysanthemum sp. Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from protein sequence of Arabidopsis thaliana CO protein, UniProt: Q39057, TAIR: At5g15840
COI1 (Coronate insensitive 1) is an esential component of jasmonate receptor. Required for jasmonate-regulated plant fertility and defense processes, and for coronatine and/or other elicitors perceptions/responses. Alternative names: F-box/LRR-repeat protein 2, AtCOI1, AtFBL2.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized in PBS pH 7.4
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica rapa, Glycine max, Medicago tribuloides, Nicotiana tabacum, Oryza sativa, Pisum sativum, Populus trichocarpa, Ricinus communis, Solanum lycopersicum, Vitis vinifera Species of your interest not listed? Contact us
Conglutin gamma, an oligomeric protein, is one of storage proteins of lupin seeds, called also lupin-specific globulin.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Villa et al. (2020). Immunoreactivity of Lupine and Soybean Allergens in Foods as Aected by Thermal Processing. Foods. 2020 Feb 27;9(3). pii: E254. doi: 10.3390/foods9030254.Tomczak et al. (2019). Differences in the immunoreactivity of milk from local farms and from points of purchase. Eur Food Res Technol, Nov 2019.Foley et al. (2015). Analysis of conglutin seed storage proteins across lupin species using transcriptomic, protein and comparative genomic approaches. BMC Plant Biol. 2015 Apr 19;15:106. doi: 10.1186/s12870-015-0485-6.Czubiński et al. (2015). Digestion susceptibility of seed globulins isolated from different lupin species. European Food Research and Technology pp 1-13.
Cor14b is a nuclear encoded protein localized in chloroplast whose expression level has been associated with frost and photoinhibition tolerance in monocots.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Hordeum vulgare
Immunogen:
KLH-conjugated synthetic peptide conserved in cor14b sequence of Hordeum vulgare, cor14c UniProt Q8H0Q4 and Triticum aestivum, REP13 of Secale cereale
collection of antibodies to various plant stress proteinsPlant protein extraction buffer Secondary antibodies | AgriseraSuperDeal
Molecular Weight:
16 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Rapacz et al. (2008). The effect of cold acclimation on photosynthetic apparatus and the expression of COR14b in four genotypes of barley (Hordeum vulgare) contrasting in their tolerance to freezing and high light treatment in cold. Ann. Bot. 101: 689-699.
COX11 (cytochrome c oxidase assembly protein) , involved in respiratory chain complex IV assembly.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardii
Expected Species:
Physcomitrella patens Species of your interest not listed? Contact us
Immunogen:
Recombinant fragment of Chlamydomonas reinhardtii COX11 UniProt: A8JCS0
Cytochrome c oxidase (COX) catalyzes the reduction of oxygen to water in the respiratory chain in the inner mitochondrial membrane. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 (COXII) transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Alternative name: cytochrome c oxidase subunit 2
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized in PBS pH 7.4
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Cucumis melo, Glycine max, Oryza sativa, Physcomitrella patens, Pisum sativum, Triticum aestivum, Vigna radiata Species of your interest not listed? Contact us
Immunogen:
KLH-conugated synthetic peptide fully conserved in all available protein sequences from eudicots including Arabidopsis thaliana AtmG00160, monocots including Oryza sativa P04373 and Physcomitrella patens Q1XGA9
Antibody detects COXII protein most optimally in membrane fractions. The signal is weak in a in total protein extract.Blue Native gel electrophoresis (BN-PAGE) has been performed on samples solubilized with digitonin (4:1) and loaded at 100 µg/well. Gel thickness was 2 mm with 4.5-16 % gradient.
Application Details:
1 : 1000 (BN-PAGE), 1 : 1000 (WB)
Purity:
Affinity purified serum
Reconstitution:
For reconstitution add 50 µl of sterile water.
Related products:
AS04 052 | Anti-COXII (plant), chicken antibodiesAS04 053A-200 | Anti-COXII | cytochrome oxidase subunit II (plant), rabbit antibodiesAS04 053PRE | COXII | cytochrome oxidase subunit II, pre-immune serumAS04 053P COXII | cytochrome oxidase subunit II | Blocking peptide Antibodies to other mitochondrial proteinsPlant protein extraction buffer | AgriseraSuperDeal
Molecular Weight:
29.4 | 30 kDa (for Arabidopsis thaliana)
Not reactive in:
Saccharina japonica
Selected references:
Makino et al. (2020). Induction of Terminal Oxidases of Electron Transport Chain in Broccoli Heads Under Controlled Atmosphere Storage. Foods, 9 (4) Wang et al. (2020) Rerouting of ribosomal proteins into splicing in plant organelles. BioRxiv, DOI: 10.1101/2020.03.03.974766 .Barua et al. (2019). Dehydration-responsive nuclear proteome landscape of chickpea (Cicer arietinum L.) reveals phosphorylation-mediated regulation of stress response. Plant Cell Environ. 2019 Jan;42(1):230-244. doi: 10.1111/pce.13334.Waltz et al. (2019). Small is big in Arabidopsis mitochondrial ribosome. Nat Plants. 2019 Jan;5(1):106-117. doi: 10.1038/s41477-018-0339-y.Shull et al. (2019). Anatase TiO2 nanoparticles induce autophagy and chloroplast degradation in thale cress (Arabidopsis thaliana). Environ Sci Technol. 2019 Jul 29. doi: 10.1021/acs.est.9b01648.Wang et al. (2019). SMALL KERNEL4 is required for mitochondrial cox1 transcript editing and seed development in maize. J Integr Plant Biol. 2019 Jul 23. doi: 10.1111/jipb.12856.Chen et al. (2019). PPR-SMR1 is required for the splicing of multiple mitochondrial introns and interacts with Zm-mCSF1 and is essential for seed development in maize. J Exp Bot. 2019 Jun 28. pii: erz305. doi: 10.1093/jxb/erz305.Waltz et al. (2019). Small is big in Arabidopsis mitochondrial ribosome. Nat Plants. 2019 Jan;5(1):106-117. doi: 10.1038/s41477-018-0339-y.Gayen et al. (2018). Dehydration-induced proteomic landscape of mitochondria in chickpea reveals large-scale coordination of key biological processes. J Proteomics. 2018 Sep 19. pii: S1874-3919(18)30349-X. doi: 10.1016/j.jprot.2018.09.008Barua et al. (2018). Dehydration-responsive nuclear proteome landscape of chickpea (Cicer arietinum L.) reveals phosphorylation-mediated regulation of stress response. Plant Cell Environ. 2018 May 10. doi: 10.1111/pce.13334.Migocka et al. (2018). Cucumber metal tolerance protein 7 (CsMTP7) is involved in the accumulation of Fe in mitochondria under Fe excess. Plant J. 2018 Jun 22. doi: 10.1111/tpj.14006.Dai et al. (2018). Maize Dek37 Encodes a P-Type PPR Protein That Affects Cis-splicing of Mitochondrial nad2 Intron 1 and Seed Development. Genetics. 2018 Jan 4. pii: genetics.300602.2017. doi: 10.1534/genetics.117.300602.Nagel et al. (2017). Arabidopsis SH3P2 is an ubiquitin-binding protein that functions together with ESCRT-I and the deubiquitylating enzyme AMSH3. Proc Natl Acad Sci U S A. 2017 Aug 7. pii: 201710866. doi: 10.1073/pnas.1710866114.Garmash et al. (2017). Expression profiles of genes for mitochondrial respiratory energy-dissipating systems and antioxidant enzymes in wheat leaves during de-etiolation. J Plant Physiol. 2017 Aug;215:110-121. doi: 10.1016/j.jplph.2017.05.023.Weißenberger et al. (2017). The PPR protein SLOW GROWTH 4 is involved in editing of nad4 and affects the splicing of nad2 intron 1. Plant Mol Biol. 2017 Mar;93(4-5):355-368. doi: 10.1007/s11103-016-0566-4.Cai et al. (2017). Emp10 encodes a mitochondrial PPR protein that affects the cis-splicing of nad2 intron 1 and seed development in maize. Plant J. 2017 Mar 27. doi: 10.1111/tpj.13551.Schimmeyer et al. (2016). L-Galactono-1,4-lactone dehydrogenase is an assembly factor of the membrane arm of mitochondrial complex I in Arabidopsis. Plant Mol Biol. 2016 Jan;90(1-2):117-26. doi: 10.1007/s11103-015-0400-4. Epub 2015 Oct 31.Li et al. (2016). Characterization of a novel β-barrel protein (AtOM47) from the mitochondrial outer membrane of Arabidopsis thaliana. J Exp Bot. 2016 Nov;67(21):6061-6075. Epub 2016 Oct 6.Pavlovič et al. (2016). Light-induced gradual activation of photosystem II in dark-grown Norway spruce seedlings. Biochim Biophys Acta. 2016 Feb 18. pii: S0005-2728(16)30028-7. doi: 10.1016/j.bbabio.2016.02.009.Li et al. (2015). Autophagic recycling plays a central role in maize nitrogen remobilization. Plant Cell.Ã? 2015 May;27(5):1389-408. doi: 10.1105/tpc.15.00158. Epub 2015 May 5.
Special application note:
Cellular [compartment marker] of mitochondrial inner membrane
COXII - mitochondrial cytochrome oxidase subunit II is located in inner mitochondrial membrane. The enzyme is a component of the respiratory chain complex involved in the transfer of electrons from cytochrome c to oxygen.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Immunogen:
GST fusion with the aminoacids 4-153 of the subunit 2b of the Chlamydomonas reinhardtii cytochrome oxidase, UniProt: Q9AU05
AS04 052 | Anti-plant COXII, chicken antibodiesCollection of antibodies to Chlamydomonas proteinsAlgal protein extraction buffer Secondary antibodies | AgriseraSuperDeal
Molecular Weight:
15 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Ma et al. (2020). An ortholog of the Vasa intronic gene is required for small RNA-mediated translation repression in Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A. 2020 Jan 7;117(1):761-770. doi: 10.1073/pnas.1908356117.Jokel et al. (2020). Elimination of the flavodiiron electron sink facilitates long-term H2 photoproduction in green algae. Biotechnol Biofuels. 2019 Dec 5;12:280. doi: 10.1186/s13068-019-1618-1.Upadhyaya and Jagadeeshwar Rao (2019). Reciprocal regulation of photosynthesis and mitochondrial respiration by TOR kinase in Chlamydomonas reinhardtii. Plant Direct Volume 3, Issue 11.Jokel et al. (2018). Hunting the main player enabling Chlamydomonas reinhardtii growth under fluctuating light. Plant J. 2018 Mar 25. doi: 10.1111/tpj.13897.Uhmeyer et al. (2017). Impaired Mitochondrial Transcription Termination Disrupts the Stromal Redox Poise in Chlamydomonas. Plant Physiol. 2017 Jul;174(3):1399-1419. doi: 10.1104/pp.16.00946.Volgusheva et al. (2017). Comparative analyses of H2 photoproduction in magnesium- and sulfur-starved Chlamydomonas reinhardtii cultures. Physiol Plant. 2017 Apr 7. doi: 10.1111/ppl.12576.Schulz-Raffelt et al. (2016). Hyper-accumulation of starch and oil in a Chlamydomonas mutant affected in a plant-specific DYRK kinase. Biotechnol Biofuels. 2016 Mar 8;9:55. doi: 10.1186/s13068-016-0469-2. eCollection 2016.Kropat et al. (2015). Copper economy in Chlamydomonas: Prioritized allocation and reallocation of copper to respiration vs. photosynthesis. Proc Natl Acad Sci U S A. 2015 Feb 2. pii: 201422492.Dang et al. (2014). Combined Increases in Mitochondrial Cooperation and Oxygen Photoreduction Compensate for Deficiency in Cyclic Electron Flow in Chlamydomonas reinhardtii. Plant Cell. 2014 Jul 2. pii: tpc.114.126375.Johnson et al. (2014). Proton Gradient Regulation 5-Mediated Cyclic Electron Flow under ATP- or Redox-Limited Conditions: A Study of ΔATPase pgr5 and ΔrbcL pgr5 Mutants in the Green Alga Chlamydomonas reinhardtii. Plant Physiol. 2014 May;165(1):438-52. doi: 10.1104/pp.113.233593. Epub 2014 Mar 12.
Special application note:
Cellular [compartment marker] of mitochondrial inner membrane for Chlamydomonas reinhardtiiThis product can be sold containing ProClin if requested.
CP43' is encoded by the IsiA gene. This chlorophyll (Chl)-binding protein is induced under iron deficiency conditions in certain cyanobacterial strains. It acts as dissipater of light energy protecting photosystem II from excessive excitation under iron-deficient conditions.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized in PBS pH 7.4
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
This product can be sold containing proclin if requested
Application Details:
1 : 500 -1 : 1000 (WB)
Purity:
Affinity purified serum
Reconstitution:
For reconstitution add 100 µl of sterile water.
Related products:
AS06 110 | Anti-PsbC (CP43), rabbit antibodiescollection of antibodies to other PSII proteins Algal protein extraction buffer | AgriseraSuperDeal
Molecular Weight:
37 | 27 kDa (in a Novex gel system)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Xing et al. (2017). Deletion of CGLD1 Impairs PSII and Increases Singlet Oxygen Tolerance of Green Alga Chlamydomonas reinhardtii. Front. Plant Sci., 15 December 2017.Li et al. (2017). The identification of IsiA proteins binding chlorophyll d in the cyanobacterium Acaryochloris marina. Photosynth Res. 2017 Apr 4. doi: 10.1007/s11120-017-0379-6.Cheng and He (2014). PfsR Is a Key Regulator of Iron Homeostasis in Synechocystis PCC 6803. PLoS One. 2014 Jul 10;9(7):e101743. doi: 10.1371/journal.pone.0101743. eCollection 2014.Selão et al. (2014). Subcellular Localization of Monoglucosyldiacylglycerol Synthase in Synechocystis sp. PCC6803 and Its Unique Regulation by Lipid Environment. PLoS One. 2014 Feb 6;9(2):e88153. doi: 10.1371/journal.pone.0088153. eCollection 2014.Hakkila et al. (2013). Group 2 sigma factor mutant ΔsigCDE of the cyanobacterium Synechocystis sp. PCC 6803 reveals functionality of both carotenoids and flavodiiron proteins in photoprotection of photosystem II. Plant Cell Physiol. Sept 4.Fraser et al. (2013). Photophysiological and Photosynthetic Complex Changes during Iron Starvation in Synechocystis sp. PCC 6803 and Synechococcus elongatus PCC 7942. PLoS One.
Special application note:
Peptide used to elicit this antibody is also perfectly or highly conserved in known Pcb chlorophyll a/b binding proteins from Prochlorococcus and similar proteins from other cyanobacteria. Peptide target is partially conserved in CP43/PsbC. CP43' and CP43 can be distinguished by their size.
CDPKs are large group of kinases found/existing? only in plants and protists. They are involved in decoding Ca2+ signals in many stress conditions. CDPKs are composed of 4 domains: N-terminal variable domain, kinase domain, autoinhibitory domain and calmodulin-resembling domain (Calcium binding domain) - EF-hand domain.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Hordeum vulgare, Populus sp., Triticum aestivum
Expected Species:
Brassica napus, Nicotiana tabacum, Oryza sativa, Solanum lycopersicum, Solanum tuberosum Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from Calcium-dependent protein kinase EF domain including Arabidopsis thalaiana TAIR: At4g35310 and At5g04870
CPK1 (CALCIUM-DEPENDENT PROTEIN KINASE1) is a calcium-dependent protein kinase that can phosphorylate phenylalanine ammonia lyase (PAL), a key enzyme in pathogen defense. Alternative names: AtCDPK 1, CDPK 1, Calcium-dependent protein kinase isoform AK1,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Noccaea caerulescen, Triticum sp. Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from Arabidopsis thaliana CPK1 protein sequence, UniProt: Q06850-1, TAIR AT5G04870
CPN60 alpha 1 (chaperonin 60 subunit alpha 1, chloroplastic) binds small and large subunits of Rubisco. Assists in protein folding and required for proper chloroplast development. Synonymes: protein SCHLEPPERLESS, RuBisCO large subunit-binding protein subunit alpha 1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Aegilops squarrosa, Avicena marina, Brassica napus, Canavalia lineata, Narcissus pseudonarcissus, Oryza sativa, Ricinus communis, Trifolium pRatense, Triticum aestivum Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from known CPN60 sequences, including Arabidopsis thaliana UniProt P21238 . TAIR AT2G28000.The peptide is conserved in chloroplastic CPN60A1 but NOT the close relative CPN60A2.
collection of antibodies to RUBISCOcollection of antibodies to proteins involved in photosynthesis Plant protein extraction buffer Secondary antibodies | AgriseraSuperDeal
Molecular Weight:
57.1 kDa (mature protein)
Not reactive in:
Cyanobacteria, algae
Selected references:
Jiang et al. (2020). Plastid chaperone HSP90C guides precursor proteins to the SEC translocase for thylakoid transport. J Exp Bot. 2020 Aug 27;eraa399.doi: 10.1093/jxb/eraa399. Lande et al. (2019). Dehydration-induced alterations in chloroplast proteome and reprogramming of cellular metabolism in developing chickpea delineate interrelated adaptive responses. Plant Physiology and Biochemistry Volume 146, January 2020, Pages 337-348.Dogra et al. (2019). Impaired PSII proteostasis triggers an UPR-like response in the var2 mutant of Arabidopsis thaliana. J Exp Bot. 2019 Apr 16. pii: erz151. doi: 10.1093/jxb/erz151.
CpxA (Conjugative Plasmid Expression) is the 458 amino acid protein, a bacterial inner membrane histidine protein kinase, responsible for sensing misfolded proteins in the periplasm. It is part of CpxA/CpxR two component signal transduction system, where CpxR is the cognate response regulator. In sensing bacterial envelope stress, the Cpx pathway regulates the production of a number of periplasmic folding and trafficking factors to maintain outer membrane integrity. It is also responsible for global control of virulence factor expression. Alternative name: Integral membrane sensor signal transduction histidine kinase.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Yersinia pseudotuberculosis
Immunogen:
Recombinant CpxA (residues 26 to 168) over-expressed as a N-terminal fusion with His(x6)-tag, which was removed prior to immunization. The antigen originates from enteropathogenic Yersinia pseudotuberculosis YPIII; B1JQV1, NCBI annotated locus tag YPK_4133.
Plant protein extraction buffer Secondary antibodies | AgriseraSuperDeal
Molecular Weight:
51.8 | 52-53 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Special application note:
The strains were grown in LB broth until late stationary phase (absorbance at 600nm of ~1.5). To avoid interference from cross-reacting bands, aim to maximize protein separation with the region of 35 to 55 kDa. CpxA could be routinely detected from a 20 µL of sample that was derived from 1ml of pelleted bacteria resuspended in 200 µl of 1x loading buffer. Re-use of diluted working strength antibody is not recommended.
Antioxidant system works as a defense against oxidative stress. SOD (superoxide dismutase) catalyzes the dismutation of superoxide into oxygen and H202,. SODs are classified, according to their metal cofactor, as FeSOD, MnSOD, or Cu / ZnSOD. Chloroplasts generally contain Cu/ZnSOD and, in a number of plant species, FeSOD
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Cucumis melo, Glycne max, Pinus pinaster Species of your interest not listed? Contact us
Immunogen:
overexpressed Arabidopsis thaliana Cu/ZnSOD O78310, At2g28190 with an N-terminal His-tag. Purified via Ni-column. The His-tag was cleaved and the protein was recovered via ion exchange on a 10 ml resource-Q (Pharmacia) column using a NaCl gradient. Pure fractions after dialysis against PBS has been used for immunization.
Note: Antibody is recognizing 19 kDa protein Csd2 (chloroplastic enzyme) and gives also low but noticeable reactivity to Csd1 (15 kDa) a cytosolic form
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Konkolewska et al. (2020). Combined use of companion planting and PGPR for the assisted phytoextraction of trace metals (Zn, Pb, Cd). Shinozaki et al. (2020). Autophagy Increases Zinc Bioavailability to Avoid Light-Mediated ROS Production under Zn Deficiency. Plant Physiol. 2020 Jan 15. pii: pp.01522.2019. doi: 10.1104/pp.19.01522.Mermod et al. (2019). SQUAMOSA promoter-binding protein-like 7 mediates copper deficiency response in the presence of high nitrogen in Arabidopsis thaliana. Plant Cell Rep. 2019 May 15. doi: 10.1007/s00299-019-02422-0.Witzel et al. (2017). Temporal impact of the vascular wilt pathogen Verticillium dahliae on tomato root proteome. J Proteomics. 2017 Oct 3;169:215-224. doi: 10.1016/j.jprot.2017.04.008.Vuleta et al. (2016). Adaptive flexibility of enzymatic antioxidants SOD, APX and CAT to high light stress: The clonal perennial monocot Iris pumila as a study case. Plant Physiol Biochem. 2016 Mar;100:166-73. doi: 10.1016/j.plaphy.2016.01.011. Epub 2016 Jan 19Dmitrović et al. (2015). Essential oils of two Nepeta species inhibit growth and induce oxidative stress in ragweed (Ambrosia artemisiifolia L.) shoots in vitro. Acta Physiologiae Plantarum, February 2015, 37:64Tuteja et al. (2015). Heterologous expression and biochemical characterization of a highly active and stable chloroplastic CuZn-superoxide dismutase from Pisum sativum. BMC Biotechnology 2015, 15:3 doi:10.1186/s12896-015-0117-0Parys et al. (2014). Metabolic Responses to Lead of Metallicolous and Nonmetallicolous Populations of Armeria maritima. Arch Environ Contam Toxicol. 2014 Jul 29.Momčilović et al. (2014). Improved procedure for detection of superoxide dismutase isoforms in potato, Solanum tuberosum L. Acta Physiologiae Plantarum, August 2014, Volume 36, Issue 8, pp 2059-2066.Huey-wen et al. (2014). Harpin Protein, an Elicitor of Disease Resistance, Acts as a Growth Promoter in Phalaenopsis Orchids. Journal of Plant Growth Regulation May 2014.Yang et al. (2012). Quantitative proteomic analysis reveals that antioxidation mechanisms contribute to cold tolerance in plantain (Musa paradisiaca L.; ABB Group) seedlings. Mol Cell Proteomics. 2012 Dec;11(12):1853-69. doi: 10.1074/mcp.M112.022079.
Special application note:
This product can be sold containing ProClin if requested.
Curvature thylakoid 1A (CURT1A) belongs to a protein family, conserved in plants and cyanobaceria. There are four Arabidopsis thaliana CURT1 proteins: CURT1A,B,C and D. It is proposed that CURT1 proteins modify thylakoid architecture by inducing membrane curvature at grana margins.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Pisum sativum
Expected Species:
Nicotiana tabacum, Zea mays Species of your interest not listed? Contact us
Curvature thylakoid 1B (CURT1B) belongs to a protein family, conserved in plants and cyanobaceria. There are four Arabidopsis thaliana CURT1 proteins: CURT1A,B,C and D. It is proposed that CURT1 proteins modify thylakoid architecture by inducing membrane curvature at grana margins.Alternative names: Photosystem I protein P, Thylakoid membrane phosphoprotein 14 kDa, PSAP
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Nicotiana tabacum, Zea mays Species of your interest not listed? Contact us
Curvature thylakoid 1C (CURT1C) belongs to a protein family, conserved in plants and cyanobaceria. There are four Arabidopsis thaliana CURT1 proteins: CURT1A,B,C and D. It is proposed that CURT1 proteins modify thylakoid architecture by inducing membrane curvature at grana margins.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Nicotiana tabacum, Zea mays Species of your interest not listed? Contact us
Curvature thylakoid 1D (CURT1D) belongs to a protein family, conserved in plants and cyanobaceria. There are four Arabidopsis thaliana CURT1 proteins: CURT1A,B,C and D. It is proposed that CURT1 proteins modify thylakoid architecture by inducing membrane curvature at grana margins.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Nicotiana tabacum, Zea mays Species of your interest not listed? Contact us
Cytochrome c is located in inner mitochondrial membrane. It is a small heme protein which, unlike other cytochromes, is highly soluble. This protein is an essential component of the electron transport chain, where it undergoes oxidation and reduction without binding oxygen.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles and Store at -80°C. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
cytc1 and cytc2 from following species: A. theoprasi, Brassica napus, Brassica oleracea, Cannabis sativa, C. maxima, Chlamydomonas reinhardtii (peptide target partially conserved), Lupinus luteus, Medicago truncatula, Nicotiana tabacum, Oryza sativa, Ostreococcus (peptide target partially conserved), P. aurea, Physcomitrella patens, Ricinus communis, S. nigra, Solanum lycopersivum, Vitis vinifera. Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana cytochrome c protein sequence, UniProt:D7KMK0 (C-1) D7LY03 (C-2), TAIR: At1g22840 (Cytc1) and At4g10040 (Cytc2)
Dai et al. (2020). Pentatricopeptide repeat protein DEK46 is required for multi-sites mitochondrial RNA editing and maize seed development. J Exp Bot. 2020 Jul 25;eraa348.doi: 10.1093/jxb/eraa348. Wang et al. (2020) Rerouting of ribosomal proteins into splicing in plant organelles. BioRxiv, DOI: 10.1101/2020.03.03.974766 .Doronina et al. (2019). Structural and Functional Features of the Wheat Embryo Sac?s Antipodal Cells during Differentiation. Russ J Dev Biol 50, 194?208. (immunolocalization)Waltz et al. (2019). Small is big in Arabidopsis mitochondrial ribosome. Nat Plants. 2019 Jan;5(1):106-117. doi: 10.1038/s41477-018-0339-y.Rurek et al. (2018). Mitochondrial Biogenesis in Diverse Cauliflower Cultivars under Mild and Severe Drought Involves Impaired Coordination of Transcriptomic and Proteomic Response and Regulation of Various Multifunctional Proteins. Preprints 2018, 2018010276 (doi: 10.20944/preprints201801.0276.v1).Dai et al. (2018). Maize Dek37 Encodes a P-Type PPR Protein That Affects Cis-splicing of Mitochondrial nad2 Intron 1 and Seed Development. Genetics. 2018 Jan 4. pii: genetics.300602.2017. doi: 10.1534/genetics.117.300602.Opalińska et al. (2017). Identification of Physiological Substrates and Binding Partners of the Plant Mitochondrial Protease FTSH4 by the Trapping Approach. Int J Mol Sci. 2017 Nov 18;18(11). pii: E2455. doi: 10.3390/ijms18112455.Schimmeyer et al. (2016). L-Galactono-1,4-lactone dehydrogenase is an assembly factor of the membrane arm of mitochondrial complex I in Arabidopsis. Plant Mol Biol. 2016 Jan;90(1-2):117-26. doi: 10.1007/s11103-015-0400-4. Epub 2015 Oct 31.Li et al. (2016). Characterization of a novel β-barrel protein (AtOM47) from the mitochondrial outer membrane of Arabidopsis thaliana. J Exp Bot. 2016 Nov;67(21):6061-6075. Epub 2016 Oct 6.
Cytochrome c6 is known as a redox carrier of the thylakoid lumen of cyanobacteria and some eukaryotic algae that can substitute for plastocyanin in electron transfer.Cytochrome c6 is situated between the two membrane-bound complexes cytochrome b6f and photosystem I (PSI) in oxygenic photosynthesis.Cytochrome c6 (cyt c6) in Chlamydomonas reinhardtii is synthesized only under conditions of copper deficiency when plastocyanin cannot be synthesized.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Expected Species:
Chlorella vulgaris, Cladophora glomerata, Pediastrum duplexSpecies of your interest not listed? Contact us
Immunogen:
GST fusion of recombinant Chlamydomonas reinhardtii cytochrome c6 A8J9G6
Note: antibody will work better with nitrocellulose than with PVDF membrane. Recommended load per well min. 6 µg. Primary antibody incubation needs to be done over night. For detection image please refer to the reference below
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 200 µl of sterile water.
Related products:
AS06 122 | Anti-CRD1 | cyanobacterial homolog of plant CHL27 cyclase, marker of thylakoid and envelope membranes, rabbit antibodiesAS05 084 | Anti-PsbA rabbit antibodies, marker of thylakoid membraneCollection of antibodies to Chlamydomonas proteins | AgriseraSuperDeal
Molecular Weight:
15 kDa
Not reactive in:
Arabidopsis thaliana
Selected references:
Kropat et al. (2015). Copper economy in Chlamydomonas: Prioritized allocation and reallocation of copper to respiration vs. photosynthesis. Proc Natl Acad Sci U S A. 2015 Feb 2. pii: 201422492.Merchant et al. (1998) Copper-Responsive Gene Expression in Photosynthetic Microorganisms. Methods in Enzymology 297:263-279.
Special application note:
Cellular [compartment marker] of thylakoid lumen for Chlamydomonas reinhardtiiThis product can be sold containing proclin if requested.
Multi-subunit complex of cytb6/f is a crucial component for the photosynthetic electron transport chain of higher plants, green algae and cyanobacteria. This complex is catalyzing oxidation of quinols and the reduction the reduction of plastocyanin. This reaction allows to establish the proton force required for the ATP synthesis. Four major subunits build the complex: the petA gene product corresponding to a c-type cytochrome (cytf), the petB gene product corresponding to a b-type/c’-type cytochrome with three haems (cyt b6), the petD gene product (subunit IV, or suIV), and the petC gene product, corresponding to the Rieske/Iron/sulfur protein.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Reaction with cyanobacteria: Synechocystis 6803 and Synechococcus 7942 possible to obtain on total cell extract when using antibody at 1: 500 and longer exposure time.
Application Details:
1 : 2000-1 : 50 000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 µl of sterile water.
Related products:
AS08 306 | Anti-cytochrome f (higher plants), rabbit antibodiesCollection of antibodies to Arabidopsis proteinsCollection of antibodies to Chlamydomonas proteinsPlant and algal protein extraction buffer Secondary antibodies | AgriseraSuperDeal
Molecular Weight:
34 | 31-32 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Redekop et al. (2020). PsbS Contributes to Photoprotection in Chlamydomonas Reinhardtii Independently of Energy Dissipation . Biochim Biophys Acta Bioenerg . 2020 Jun 1;1861(5-6):148183.doi: 10.1016Liu et al. (2020). Acid treatment combined with high light leads to increased removal efficiency of Ulva prolifera. Algal Research,Volume 45, January 2020, 101745Storti et al. (2020). The activity of chloroplast NADH dehydrogenase-like complex influences the photosynthetic activity of the moss Physcomitrella patens. doi.org/10.1101/2020.01.29.924597Koh et al. (2019). Heterologous synthesis of chlorophyllÃ? bÃ? inÃ? Nannochloropsis salinaÃ? enhances growth and lipid production by increasing photosynthetic efficiency. Biotechnol Biofuels.Ã? 2019 May 14;12:122. doi: 10.1186/s13068-019-1462-3. eCollection 2019.Dall'Osto et al. (2019). Combined resistance to oxidative stress and reduced antenna size enhance light-to-biomass conversion efficiency in Chlorella vulgaris cultures.Biotechnol Biofuels. 2019 Sep 16;12:221. doi: 10.1186/s13068-019-1566-9.Fristedt et al. (2015). The thylakoid membrane protein CGL160 supports CF1CF0 ATP synthase accumulation in Arabidopsis thaliana. PLoS One. 2015 Apr 2;10(4):e0121658. doi: 10.1371/journal.pone.0121658. Storti et al. (2018). Role of cyclic and pseudo-cyclic electron transport in response to dynamic light changes in Physcomitrella patens. Plant Cell Environ. 2018 Nov 29. doi: 10.1111/pce.13493.Kong et al. (2018) Interorganelle Communication: Peroxisomal MALATE DEHYDROGENASE2 Connects Lipid Catabolism to Photosynthesis through Redox Coupling in Chlamydomonas. Plant Cell. 2018 Aug;30(8):1824-1847. doi: 10.1105/tpc.18.00361Jokel et al. (2018). Hunting the main player enabling Chlamydomonas reinhardtii growth under fluctuating light. Plant J. 2018 Mar 25. doi: 10.1111/tpj.13897.Du et al. (2018). Galactoglycerolipid Lipase PGD1 Is Involved in Thylakoid Membrane Remodeling in Response to Adverse Environmental Conditions in Chlamydomonas. Plant Cell. 2018 Feb;30(2):447-465. doi: 10.1105/tpc.17.00446.Schöttler et al. (2017). The plastid-encoded PsaI subunit stabilizes photosystem I during leaf senescence in tobacco. J Exp Bot.Ã? 2017 Feb 1;68(5):1137-1155. doi: 10.1093/jxb/erx009.Zou et al. (2017). An Animal-Like Cryptochrome Controls the Chlamydomonas Sexual Cycle. Plant Physiol. 2017 Jul;174(3):1334-1347. doi: 10.1104/pp.17.00493.Georg et al. (2017). Acclimation of Oxygenic Photosynthesis to Iron Starvation Is Controlled by the sRNA IsaR1. Curr Biol. 2017 May 22;27(10):1425-1436.e7. doi: 10.1016/j.cub.2017.04.010. (Synechocystis PCC6803) Tyuereva et al. (2017). The absence of chlorophyll b affects lateral mobility of photosynthetic complexes and lipids in grana membranes of Arabidopsis and barley chlorina mutants. Photosynth Res. 2017 Apr 5. doi: 10.1007/s11120-017-0376-9. (Hordeum vulgare, western blot)Ferroni et al. (2016). Light acclimation in the lycophyte Selaginella martensii depends on changes in the amount of photosystems and on the flexibility of the light-harvesting complex II antenna association with both photosystems. New Phytol. 2016 Apr 5. doi: 10.1111/nph.13939.Suorsa et al. (2015). Light acclimation involves dynamic re-organisation of the pigment-protein megacomplexes in non-appressed thylakoid domains. Plant J. 2015 Aug 29. doi: 10.1111/tpj.13004.Charuvi et al. (2015). Photoprotection Conferred by Changes in Photosynthetic Protein Levels and Organization during Dehydration of a Homoiochlorophyllous Resurrection Plant. Plant Physiol. 2015 Apr;167(4):1554-65. doi: 10.1104/pp.114.255794.Hojka et al. (2014). Inducible repression of nuclear-encoded subunits of the cytochrome b6f complex in tobacco reveals an extraordinarily long lifetime of the complex. Plant Physiol. 2014 Jun 24. pii: pp.114.243741.Dang et al. (2014). Combined Increases in Mitochondrial Cooperation and Oxygen Photoreduction Compensate for Deficiency in Cyclic Electron Flow in Chlamydomonas reinhardtii. Plant Cell. 2014 Jul 2. pii: tpc.114.126375.
Multi-subunit complex of cytb6/f is a crucial component for the photosynthetic electron transport chain of higher plants, green algae and cyanobacteria. This complex is catalyzing oxidation of quinols and the reduction the reduction of plastocyanin. This reaction allows to establish the proton force required for the ATP synthesis. Four major subunits build the complex: the petA gene product corresponding to a c-type cytochrome (cytf), the petB gene product corresponding to a b-type/c’-type cytochrome with three haems (cyt b6), the petD gene product (subunit IV, or suIV), and the petC gene product, corresponding to the Rieske/Iron/sulfur protein.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
DCL1 (EC=3.1.26) is a ribonuclease (RNase) III involved in RNA-mediated post-transcriptional gene silencing (PTGS). Functions in the microRNAs (miRNAs) biogenesis pathway by cleaving primary miRNAs (pri-miRNAs) and precursor miRNAs (pre-miRNAs). Synonymes: Protein ABNORMAL SUSPENSOR 1, Protein CARPEL FACTORY, Protein SHORT, INTEGUMENTS 1, Protein SUSPENSOR 1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
DCL3 (EC=3.1.26) is a ribonuclease (RNase) III involved in RNA-mediated post-transcriptional gene silencing (PTGS). Functions in the microRNAs (miRNAs) biogenesis pathway by cleaving primary miRNAs (pri-miRNAs) and precursor miRNAs (pre-miRNAs). Synonymes: Endoribonuclease Dicer homolog 3.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
DEG15 is a peroxisomal Deg-protease with endopeptidase activity. This protease cleaves specifically substrates with Cys in the P1 and P2 position and acts as peroxisomal processing peptidase. Alternative names: At1g28320/F3H9_2
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Micromonas sp., Oryza sativa, Populus balsamifera, Solanum lycopersicum, Sorghum vulgare, Ricinus communis, Vitis vinifera, Zea mays Species of your interest not listed? Contact us
Immunogen:
synthetic peptide derived from Arabidopsis thaliana DEG15, Q8VZD4 (At1g28320)
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Schuhmann et al. (2008). The DEG15 serine protease cleaves peroxisomal targeting signal 2-containing proteins in Arabidopsis.Plant Physiol. 4: 1847-1856.Helm et al. (2007). Dual specificities of the glyoxysomal/peroxisomal processing protease Deg15 in higher plants.PNAS 27: 11501-11506.
Protease Do-like 7 (DegP7) EC=3.4.21 is a serine/cysteine protease involved in photoiunhibition process.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Brassica oleracea
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from known Arabidopsis thaliana DegP7 UniProt: Q8RY22-1 , TAIR: At3g03380
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Schuhmann et al. (2011). A new principle of oligomerization of plant DEG7 protease based on interactions of degenerated protease domains. Biochem. J. 435:167-174.
Dehydrins are stress proteins involved in formation of plant protective reactions against dehydration. They are normally synthesized in maturating seeds during their dessication, as well as in vegetative tissues of plants treated with abscisic acid or exposed to environmental stress factors that result in cellular dehydration.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Brassica oleracea, Cucumis sativus, Lycopersicon esculentum, Pinus sylvestris, Pistacia vera
Expected Species:
Glycine max, Hordeum vulgare, Nicotiana tabacum, Oryza sativa, Pisum sativum, Populus sp., Zea mays Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide sequence TGEKKGIMDKIKEKLPGQH of K-segment conserved in a wide range of plant species
For reconstitution add 50 µl of sterile, deionized water.
Related products:
AS07 206 | Anti-dehydrin rabbit antibodies (serum)AS10 206S | Dehydrin blocking peptidecollection of antibodies to plant stress proteins | AgriseraSuperDeal
Molecular Weight:
9-200 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
ZamaniBahramabadi et al. (2019). Dehydrin Content in Fresh and Desiccated Pistachio (Pistacia vera L.) Seeds. Iranian Journal of Science and Technology, Transactions A: Science, pp 1–7.Szegő et al. (2019). Diverse responsiveness of dehydrin genes to abscisic acid and water stress treatments in cucumber F1 cultivar hybrids. The J of Horticult Science and Biotechnology. June 2019. Goñi et al. (2018). Ascophyllum nodosum extract biostimulants and their role in enhancing tolerance to drought stress in tomato plants. Plant Physiol Biochem. 2018 May;126:63-73. doi: 10.1016/j.plaphy.2018.02.024.
DnaK2 belongs to the family of heat shock proteins 70 and functions as a chaperone involved in stress response. Dnak2 is heat induced but also present at high level under normal growth conditions.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Synechocystis PCC 6803
Expected Species:
Anabaena sp., Crocosphaera watsonii, Cyanothece sp. PCC 7424, Lyngbya sp. PCC 8106, Microcoleus chthonoplastes PCC 7420, Nodularia spumigena CCY 9414, Nostoc sp., Synechococcus sp. PCC 7942 and other cyanobacterial strains Species of your interest not listed? Contact us
Immunogen:
full length recombinant DnaK2 protein derived from Synechocystis PCC 6803 DnaK2 protein sequence UniProt: P22358
DRP5B (dynamin related protein 5B), EC=3.6.5.5 is involved in plastid and peroxisome division process and required for the last steps of plastid division, especially in mesophyll-cell. Necessary for peroxisome activities. Alternative names: Dynamin-like protein ARC5, Dynamin-related protein 5B, Protein ACCUMULATION AND REPLICATION OF CHLOROPLASTS 5.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Glycne max, Manihot esculenta, Mesostigma viride, Oryza sativa, Physcomitrella patens, Sorghum bicolor, Triticum aestivum, Zea mays, Vitis vinifera Species of your interest not listed? Contact us
EDS1 (Enhanced disease susceptibility 1) is a protein with lipase activity involved in aerencyma formation, lipid metabolic process, response to hypoxia and systemic acquired resistance. Alternative names: Putative disease resistance protein EDS1, Putative uncharacterized protein T17F15.40.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized in PBS pH 7.4
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
collection of antibodies to pathogen attackcollection of antibodies to hypoxiaPlant protein extraction buffer Secondary antibodies | AgriseraSuperDeal
Molecular Weight:
71.6 kDa | 72 kDa
Not reactive in:
Nicotiana benthamiana
Selected references:
Chang et al. (2019). PBS3 Protects EDS1 from Proteasome-Mediated Degradation in Plant Immunity. Mol Plant. 2019 Feb 11. pii: S1674-2052(19)30055-3. doi: 10.1016/j.molp.2019.01.023.Chakraborty et al. (2018). Epigenetic and transcriptional control of chickpea WRKY40 promoter activity under Fusarium stress and its heterologous expression in Arabidopsis leads to enhanced resistance against bacterial pathogen. Plant Science, doi.org/10.1016/j.plantsci.2018.07.014
Special application note:
This product can be sold containing ProClin if requested
EF1A (elongation factor 1-alpha) belongs to the GTP-binding elongation factor family and is localized in the cytoplasm. It is an essential enzyme in elongation phase of protein synthesis. There are four genes encoding EF1A in Arabidopsis, sequences of genes 1,2 and 3 are identical. Synonymes:eEF-1A.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
This product can be sold containing ProClin if requested.Following protein resolubilization with urea/thiourea/CHAPS signal strength of recognized band is decreased. Recommended protein extraction conditions are without urea.
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 200 µl of sterile water.
Related products:
AS11 1633 | Anti-EF1A | elongation factor 1-alpha / EF-1-alpha, rabbit antibodies made to a recombinant EF1A from Arabidopsis thalianaAS10 679 | Anti-eEF1B-alpha2 | elongation factor 1B-alpha 2, rabbit antibodiesAS10 678 | Anti-eEF1B-alpha1 an 2 | elongation factor 1B-alpha 1 and 2, rabbit antibodiesAS10 677 | Anti-eEF1B-beta1 and 2 | elongation factor 1-beta1 and 1-beta2, rabbit antibodiesAS07 265 | Anti-eEF1b | elongation factor eEF1b-beta protein, rabbit antibodiesAS10 676 | Anti-eEF1B-gamma1 and 2 | elongation factor 1-gamma 1 and 2, rabbit antibodiesPlant and algal protein extraction buffer Secondary antibodies | AgriseraSuperDeal
Molecular Weight:
49.5 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Ren et al. (2020). GPA5 Encodes a Rab5a Effector Required for Post-Golgi Trafficking of Rice Storage Proteins. Plant Cell. 2020 Jan 16. pii: tpc.00863.2019. doi: 10.1105/tpc.19.00863.Djukić et al. (2019). Expression of protein synthesis elongation factors in winter wheat and oat in response to heat stress. Journal of Plant Physiology Volume 240, September 2019, 153015.Foley et al. (2017). A Global View of RNA-Protein Interactions Identifies Post-transcriptional Regulators of Root Hair Cell Fate.Dev Cell. 2017 Apr 24;41(2):204-220.e5. doi: 10.1016/j.devcel.2017.03.018.
eEF1B-beta protein belongs to a family of elongation factors, proteins which are involved in translational elongation. eEF1B-beta protein is localized in plant cytosol. Alternative names: EF-1-delta 2, elongation factor 1B-beta 2
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Zea mays
Expected Species:
Brassica campestris, Hordeum vulgare, Oryza sativa Pisum sativum, Ricinus communis Species of your interest not listed? Contact us
Immunogen:
Recombinant eEF1B-beta protein from Arabidopsis thaliana with no affinity tag, UniProt: Q9SI20,TAIR: At2g18110
AS11 1633 | Anti-EF1A | elongation factor 1-alpha / EF-1-alpha, rabbit antibodiesAS10 679 | Anti-eEF1B-alpha2 | elongation factor 1B-alpha 2, rabbit antibodiesAS10 678 | Anti-eEF1B-alpha1 an 2 | elongation factor 1B-alpha 1 and 2, rabbit antibodiesAS10 677 | Anti-eEF1B-beta1 and 2 | elongation factor 1-beta1 and 1-beta2, rabbit antibodiesAS10 676 | Anti-eEF1B-gamma1 and 2 | elongation factor 1-gamma 1 and 2, rabbit antibodiescollection of antibodies to DNA/RNA metabolism | AgriseraSuperDeal
Molecular Weight:
25 | 30 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Lázaro-Mixteco et al. (2012). The Absence of Heat Shock Protein HSP101 Affects the Proteome of Mature and Germinating Maize Embryos. J. Proteome Res. May 11, ahead of print.
EF-G1 (elongation factor G1) is catalyzing the GTP-dependent ribosomal translocation step during translation elongation. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. Portein is located in cytoplasm.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
AS11 1633 | Anti-EF1A | elongation factor 1-alpha / EF-1-alpha, rabbit antibodiesAS10 678 | Anti-eEF1B-alpha1 an 2 | elongation factor 1B-alpha 1 and 2, rabbit antibodiesAS10 677 | Anti-eEF1B-beta1 and 2 | elongation factor 1-beta1 and 1-beta2, rabbit antibodiesAS07 265 | Anti-eEF1b | elongation factor eEF1b-beta protein, rabbit antibodiesAS10 676 | Anti-eEF1B-gamma1 and 2 | elongation factor 1-gamma 1 and collection of antibodies to DNA/RNA metabolism Algal protein extraction bufferSecondary antibodies | AgriseraSuperDeal
Molecular Weight:
76.7 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Bárria et al. (2019). Pneumococcal RNase R globally impacts protein synthesis by regulating the amount of actively translating ribosomes. RNA Biol. 2019 Feb;16(2):211-219. doi: 10.1080/15476286.2018.1564616.
EF1A (elongation factor 1-alpha) belongs to the GTP-binding elongation factor family and is localized in the cytoplasm. It is an essential enzyme in elongation phase of protein synthesis. There are four genes encoding EF1A in Arabidopsis, sequences of genes 1,2 and 3 are identical. Synonymes:eEF-1A.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
AS10 679 | Anti-eEF1B-alpha2 | elongation factor 1B-alpha 2, rabbit antibodiesAS10 678 | Anti-eEF1B-alpha1 an 2 | elongation factor 1B-alpha 1 and 2, rabbit antibodiesAS10 677 | Anti-eEF1B-beta1 and 2 | elongation factor 1-beta1 and 1-beta2, rabbit antibodiesAS07 265 | Anti-eEF1b | elongation factor eEF1b-beta protein, rabbit antibodiesAS10 676 | Anti-eEF1B-gamma1 and 2 | elongation factor 1-gamma 1 and 2, rabbit antibodiesPlant protein extraction buffer | AgriseraSuperDeal
Molecular Weight:
49.5 kDa
Not reactive in:
Recombinant wheat EF1A
Selected references:
Djukić et al. (2019). Resolving subcellular plant metabolism. Plant J. 2019 Jul 30. doi: 10.1111/tpj.14472.Zhen et al. (2018). 2D-DIGE comparative proteomic analysis of developing wheat grains under high-nitrogen fertilization revealed key differentially accumulated proteins that promote storage protein and starch biosyntheses. Anal Bioanal Chem. 2018 Jul 30. doi: 10.1007/s00216-018-1230-4.Wang et al. (2016). GOLGI TRANSPORT 1B Regulates Protein Export from the Endoplasmic Reticulum in Rice Endosperm Cells. Plant Cell. 2016 Nov;28(11):2850-2865. (Oryza sativa, western blot)
EF1A (elongation factor 1-alpha) belongs to the GTP-binding elongation factor family and is localized in the cytoplasm. It is an essential enzyme in elongation phase of protein synthesis. There are four genes encoding EF1A in Arabidopsis, sequences of genes 1,2 and 3 are identical. Synonymes: eEF-1A.
Product Type:
Antibody
Format:
Liquid
Storage Temp:
Store at -20°C
Immunogen:
full length, recombinant EF1A of Arabidopsis thaliana UniProt: P0DH99-1
eIF4A (Eukaryotic initiation factor eIF4A) is involved in protein biosynthesis.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
EIN2 (Ethylene-insensitive protein 2) is a central factor in signaling pathways regulated by ethylene including various processes like: plant defense and development, senescence, nucleotide sugar flux and tropisms. Alternative names: EIN2, ETHYLENE INSENSITIVE 2, PIR2, CKR1, CYTOKININ RESISTANT 1, ERA3, ENHANCED RESPONSE TO ABA3, ORE3, ORESARA 3, ORE2, ORESARA 2, ATEIN2.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brachypodium distachyon, Cucumis sativus, Glycine max, Hordeum vulgare, Medicago truncatula, Nicotiana tabacum, Solanum lycopersicum, Oryza sativa, Phtheirospermum japonicum, Populus trichocarpa, Ricinus communis, Solanum lycopresicum, Sorghum bicolor, Triticum aestivum, , Zea mays, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide chosen from EIN2 of Arabidopsis thaliana, UniProt: Q9S814, TAIR: AT5G03280Chosen peptide is not to be found in EIN3.
EIN3 (Ethylene insensitive 3) acts as a positive regulator in the ethylene response pathway and is required for ethylene responsiveness in adult plant tissues. Activated by phosphorylation by MPK3 and MPK6 (PubMed:18273012). Down-regulated by KIN10 that controls its protein stability under a phosphorylation-dependent manner. Alternative names: Protein ethylene insensitive 3.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Immunogen:
Recombinant EIN3 of Arabidopsis thaliana , UniProt: O24606-1, TAIR: At3g20770
Western blot condtions for anti-EIN3 antibodies are described in Smalle et al. 2002.Recommended membrane for blocking: nitrocellulose, blocking reagent: 10 % non-fat milk, antibody incubation buffer: PBS-T.
Gagne et al. (2004). Arabidopsis EIN3-binding F-box 1 and 2 form ubiquitin-protein ligases that repress ethylene action and promote growth by directing EIN3 degradation. Proc Natl Acad Sci U S A. 2004 Apr 27;101(17):6803-8.
Early light-induced proteins (ELIPs) are light stress-induced proteins related to the chlorophyll a/b binding protein family from higher plants and green algae located in the thylakoid membranes and involved in photosynthesis.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Min. 30 µg of total protein has to be loaded per lane. Please note that level of Elip proteins varies considerably between different plant species and therefore protein load/well has to be adjusted.
Application Details:
1 : 500-1 : 1000 (WB)
Purity:
Affinity purified serum in PBS pH 7.4
Reconstitution:
For reconstitution add 50 µl of sterile water.
Related products:
AS03 036 | Anti-Elip2 | early light inducible protein 2, rabbit antibodiescollection of antibodies to proteins involved in photosynthesisPlant protein extraction buffer Secondary antibodies | AgriseraSuperDeal
Early light-induced proteins (ELIPs) are light stress-induced proteins related to the chlorophyll a/b binding protein family from higher plants and green algae located in the thylakoid membranes and involved in photosynthesis.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Immunogen:
Short peptide chosen from a sequence of early light-induced protein 2 of Arabidopsis thaliana AAD28779.1
Western blot images are presented in respective publications.
Application Details:
1 : 500 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 200 µl of sterile water.
Related products:
Photosynthetic antibody collectionPlant protein extraction buffer | AgriseraSuperDeal
Molecular Weight:
21 kDa
Not reactive in:
Other plant species than Arabidopsis thaliana
Selected references:
Yao et al. (2015). Ultraviolet-B protection of ascorbate and tocopherol in plants related with their function on the stability on carotenoid and phenylpropanoid compounds. Plant Physiology and Biochemistry Volume 90, May 2015, Pages 23–31.Andersson et al. (2003). Light stress-induced one-helix protein of the chlorophyll a/b-binding family associated with photosystem I. Plant Physiol. 132:811-820.Heddad & Adamska (2000). Light stress-regulated two-helix proteins in Arabidopsis thaliana related to the chlorophyll a/b-binding gene family. PNAS 97:3741-3746.
Special application note:
To obtain a signal with this antibody plants have to be exposed to a fluorescent light source HQI-E bulb 400W/D, above 800 mE.
EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase, chloroplastic) is an enzyme (EC:2.5.1.19)which is involved in glyphosate metabolic process. Localized in chloroplast stroma. Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate. Alternative name: 5-enolpyruvylshikimate-3-phosphate synthase.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arachis hypogaea, Cannabis sativa, Coffea arabica, Cucumis sativus, Glycine max, Glycine soja, Malus domestica, Nicotiana tabacum, Zea mays, Vitis vinifera Species of your interest not listed? Contact us
ERD7 (Early Response to Dehydration ) and its homologs are important for plant stress responses and development and associated with modification of membrane lipid composition.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
To induce detactable levels of ERD7. plants need to be exposed to low temperature of 4 ºC for 24 h.
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 µl, of sterile water.
Related products:
collection of antibodies to proteins involved in the stress response | AgriseraSuperDeal
Molecular Weight:
49 | 58 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Dios Barajas-Lopez et al. (2020). EARLY RESPONSE TO DEHYDRATION 7 Remodels Cell Membrane Lipid Composition During Cold Stress in Arabidopsis. Plant Cell Physiol. 2020 Nov 3;pcaa139. doi: 10.1093/pcp/pcaa139.
FBPase1 (Fructose-1,6-bisphosphatase 1, chloroplastic (chloroplastic marker in photosynthetic tissues) involved in carbohydrate biosynthesis. Alternative names: D-fructose-1,6-bisphosphate 1-phosphohydrolase, HCEF1 (High Cyclic Electron Flow 1).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Abrus precatorius, Actinidia chinensis, Arabis nemorensis, Beta vulgaris, Brassica napus, Capsella rubella, Cephalotus follicularis, Eucalyptus grandis, Gossypium tomentosum, Hibiscus syriacus, Manihot esculenta, Morella rubra, Mucuna pruriens, Nelumbo nucifera, Parasponia andersonii, Populus sp., Prunus dulcis, Prunus persica, Salvia splendens, Syzygium oleaosum, Vitis vinifera Species of your interest not listed? Contact us
Antioxidant system works as a defense against oxidative stress. SOD (superoxide dismutase) catalyzes the dismutation of superoxide into oxygen and H,O,. SODs are classified, according to their metal cofactor, as FeSOD, MnSOD, or Cu / ZnSOD. Chloroplasts generally contain Cu/ZnSOD and, in a number of plant species, FeSOD
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Algae, Dunaliella salina, Glycine max, Helianthus annuus, Nannochloropsis gaditana, Solanum lycopersicum, Physcomitrella patens, Pinus pinaster, Populus balsamifera, Volvox carteri Species of your interest not listed? Contact us
Immunogen:
overexpressed Chlamydomonas reinhardtii thioredoxine fusion protein A8IGH1, FeSOD excised from a gel piece
The antibody will detect FeSOD enzyme only in plants grown on low Cu (0.1 μM).Reference: Salah et al (2005) Two P-type ATPases are required for copper delivery in Arabidopsis thaliana chloroplasts. Plant Cell, 17, 1233-1251Out of three FeSOD isoforms, FeSOD2 and FeSOD3 are not expressed in the roots. In roots of Arabidopsis thaliana, FeSOD1 is detected Takáč et al. (2018)This product can be sold containing ProClin if requested
Application Details:
1 : 1500-1 : 5000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 µl of sterile water.
Related products:
AS06 170 | Anti-CSD2 | chloroplastic Cu/Zn superoxide dismutase, rabbit antibodiesAS07 219 | Anti-CCS | chloroplastic copper chaperone for SOD, rabbit antibodiesAS09 524 | Anti-MnSOD | manganese superoxide dismutase, rabbit antibodiesCollection of antibodies to Arabidopsis proteinsCollection of antibodies to Chlamydomonas proteinsPlant and algal protein extraction buffer | AgriseraSuperDeal
Molecular Weight:
25 | 22 kDa
Not reactive in:
Cyanobacteria
Selected references:
Konkolewska et al. (2020). Combined use of companion planting and PGPR for the assisted phytoextraction of trace metals (Zn, Pb, Cd). Jokel et al. (2020). Elimination of the flavodiiron electron sink facilitates long-term H2 photoproduction in green algae. Biotechnol Biofuels. 2019 Dec 5;12:280. doi: 10.1186/s13068-019-1618-1.Shull et al. (2019). Anatase TiO2 nanoparticles induce autophagy and chloroplast degradation in thale cress (Arabidopsis thaliana). Environ Sci Technol. 2019 Jul 29. doi: 10.1021/acs.est.9b01648.Mermod et al. (2019). SQUAMOSA promoter-binding protein-like 7 mediates copper deficiency response in the presence of high nitrogen in Arabidopsis thaliana. Plant Cell Rep. 2019 May 15. doi: 10.1007/s00299-019-02422-0.Chen et al. (2018). The molecular chaperon AKR2A increases the mulberry chilling-tolerant capacity by maintaining SOD activity and unsaturated fatty acids composition. Sci Rep. 2018 Aug 14;8(1):12120. doi: 10.1038/s41598-018-30379-9.Bastow et al. (2018). Vacuolar Iron Stores Gated by NRAMP3 and NRAMP4 Are the Primary Source of Iron in Germinating Seeds. Plant Physiol. 2018 Jul;177(3):1267-1276. doi: 10.1104/pp.18.00478.Hura et al. (2018). Rieske iron-sulfur protein of cytochrome-b6f is involved in plant recovery after drought stress. Rieske iron-sulfur protein of cytochrome-b6f is involved in plant recovery after drought stress.Balážová et al. (2018). Zinc oxide nanoparticles phytotoxicity on halophyte from genus Salicornia. Plant Physiol Biochem. 2018 Sep;130:30-42. doi: 10.1016/j.plaphy.2018.06.013.Jokel et al. (2018). Hunting the main player enabling Chlamydomonas reinhardtii growth under fluctuating light. Plant J. 2018 Mar 25. doi: 10.1111/tpj.13897.Volgusheva et al. (2017). Comparative analyses of H2 photoproduction in magnesium- and sulfur-starved Chlamydomonas reinhardtii cultures. Physiol Plant. 2017 Apr 7. doi: 10.1111/ppl.12576.Momčilović et al. (2014). Improved procedure for detection of superoxide dismutase isoforms in potato, Solanum tuberosum L. Acta Physiologiae Plantarum, August 2014, Volume 36, Issue 8, pp 2059-2066.Dang et al. (2014). Combined Increases in Mitochondrial Cooperation and Oxygen Photoreduction Compensate for Deficiency in Cyclic Electron Flow in Chlamydomonas reinhardtii. Plant Cell. 2014 Jul 2. pii: tpc.114.126375.
FIE (fertilization-independent endosperm) belongs to polycomb group (PcG) protein which act by forming multiprotein complexes, required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Recombinant FIE protein
Expected Species:
Arabidopsis thaliana, Triticum aestivum Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana FIE protein sequence, UniProt: Q9LT47, TAIR: At3g20740
This antibody so far has been confirmed to detect recombinant FIE protein, GFP-tagged. Optimization of protein extraction and detection using most sensitive reagents may result in recognition of endogenous FIE.
Application Details:
1 : 1000 (WB)
Purity:
Affinity purified serum in PBS, pH 7.4
Reconstitution:
For reconstitution add 50 µl of sterile water.
Related products:
collection of antibodies to proteins involved in plant developmentPlant protein extraction buffer Secondary antibodies | AgriseraSuperDeal
Molecular Weight:
41 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
FLS2 (Flagellin-sensitive 2) is a pattern-recognition receptor and a single-pass membrane protein. Alternative names: FLAGELLIN-SENSITIVE 2, FLAGELLIN-SENSING 2, FLS2, LRR receptor-like serine/threonine-protein kinase FLS2
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Capsella rubella, Glycine max, Hordeum vulgare, Populus trichocarpa, Solanum lycopersivum, Vitis vinifera Species of your interest not listed? Contact us
AS12 1858 | Anti-BAK1 | Brassinosteroid insensitive 1-associated receptor kinase 1, rabbit antibodies collection of antibodies involved in response pathogen attackPlant protein extraction buffer Secondary antibodies | AgriseraSuperDeal
Molecular Weight:
126 kDa (without propeptide)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Hilleary et al. (2020). Tonoplast-localized Ca2+ pumps regulate Ca2+ signals during pattern-triggered immunity in Arabidopsis thaliana. Proc Natl Acad Sci U S A . 2020 Aug 4;117(31):18849-18857.doi: 10.1073/pnas.2004183117. Zhang et al. (2019). An important role of L -fucose biosynthesis and protein fucosylation genes in Arabidopsis immunity. New Phytol. 2018 Dec 15. doi: 10.1111/nph.15639.Yang et al. (2019). A Plant Immune Receptor Degraded by Selective Autophagy. Mol Plant. 2019 Jan 7;12(1):113-123. doi: 10.1016/j.molp.2018.11.011.Zhang et al. (2018). An important role of L -fucose biosynthesis and protein fucosylation genes in Arabidopsis immunity. New Phytol. 2018 Dec 15. doi: 10.1111/nph.15639.Zhang et al. (2018). The MAP4 Kinase SIK1 Ensures Robust Extracellular ROS Burst and Antibacterial Immunity in Plants. Cell Host Microbe. 2018 Sep 12;24(3):379-391.e5. doi: 10.1016/j.chom.2018.08.007.Lal et al. (2018). The Receptor-like Cytoplasmic Kinase BIK1 Localizes to the Nucleus and Regulates Defense Hormone Expression during Plant Innate Immunity. Cell Host Microbe. 2018 Apr 11;23(4):485-497.e5. doi: 10.1016/j.chom.2018.03.010.
FNR (ferredoxin-NADP+-oxidoreductase) catalyzes reduction of NADP+ using Fd that has accepted electrons from photosystem II in the final step of linear photosynthetic electron transport. In higher plants FNRs are encoded by a small multiple gene family (two chloroplast-targeted FNR in Arabidopsis and rice and three isoenzymes in maize). All forms are evenly distributed between the thylakoids and stroma.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
For detection in Chlorella sorokiniana, Synechococcus PCC 6803 higher load per well needs to be applied.This antibody recognizes all Arabidopsis thaliana FNR isoforms.This product can be sold with ProClin if requested
Application Details:
1 : 1000-1 : 3000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 µl of sterile water.
Related products:
Other antibodies to proteins involved in photosynthetic electron transferSecondary antibodies | AgriseraSuperDeal
Molecular Weight:
35 kDa
Not reactive in:
Oryza sativa, Spinacia oleracea, Zea mays
Selected references:
Zhang et al. (2020). Enhanced Relative Electron Transport Rate Contributes To Increased Photosynthetic Capacity In Autotetraploid Pak Choi. Plant Cell Physiol. 2020 Jan 6. pii: pcz238. doi: 10.1093/pcp/pcz238.
Special application note:
Arabidopsis has four FNR proteins, two of them are found in leaves (LFNR1 and LFNR2) while the other two in roots (RFNR1 and RFNR2). Absence of one of leaf FNR results in a decrease in the amount of FNR while absence of both of them is lethal.
FNRL | Ferredoxin-NADP+ oxidoreductase-like (FNRs; EC:1.18.1.2) is a member of the plant-type FNR family. It contains a two-domain scaffold that forms the basis of an extended superfamily of flavin adenine dinucleotide (FAD) dependent oxidoreductases. FNRL is soluble protein in chloroplast stroma.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica cretica, Capsella rubella, Eutrema salsugineum, Noccaea caerulescens Species of your interest not listed? Contact us
AS08 306 | Anti-Cyt f | Cytochrome f protein (PetA) of thylakoid Cyt b6/f-complex (higher plants), rabbit antibodiesAS06 121 | Anti-FDX1 | Ferredoxin, rabbit antibodies AS06 141 | Anti-PC | Plastocyanin, rabbit antibodiesCollection of antibodies to proteins involved in electron transfer | AgriseraSuperDeal
Molecular Weight:
31.9 | 28 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Koskela et al. (2018). Arabidopsis FNRL protein is an NADPH-dependent chloroplast oxidoreductase resembling bacterial ferredoxin-NADP+ reductases. Physiol Plant. 2018 Feb;162(2):177-190. doi: 10.1111/ppl.12621.
In angioperms the 20 kDa protein FT (coded by the gene flowering locus T) promotes flowering under long-day conditions. It has been shown to contribute to flowering initiation upon its expression in leafs followed by transport to apical meristems.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Betula luminifera, Brassica napa, Brassica oleracea, Eucalyptus sp., Hordeum vulgare, Jatropha curcas, Nicotiana tabacum, Oryza sativa, Persea americana var. americana, Populus tomentosa, Solanum tuberosum, Zea mays Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived fromA.thaliana FT protein sequence(Q9SXZ2, At1g65480); please note that this antibody will cross-react with the highly homologous TSF (twin sister of FT) protein
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Nakamura et al. (2019). High-Resolution Crystal Structure of Arabidopsis FLOWERING LOCUS T Illuminates Its Phospholipid-Binding Site in Flowering. iScience. 2019 Nov 22;21:577-586. doi: 10.1016/j.isci.2019.10.045.Liang and Ow et al. (2019). Nucleocytoplasmic OXIDATIVE STRESS 2 can relocate FLOWERING LOCUS T. Biochemical and Biophysical Research Communications Volume 517, Issue 4, 1 October 2019, Pages 735-740
FtsH belong to a family of ATP dependent peptidases. Localized in a chloroplast are following isoforms: FTSH1 (synonymes AAA, FTSH, FTSH Protease 1), Ftsh2 (VAR2, VARIEGATED 2), FtsH5 (VAR1, VARIEGATED 1), FtsH6 (FTSH PROTEASE 6), FtsH7, FtsH8. FtsH9.Localized in mitochondria are following isoforms: FtsH3 (FTSH Protease 3), FtsH4, FtsH10, FtsH11.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Diatoms including Phaeodactylum tricornutum, dicots, Chlamydomonas reinhardtii, Monocots; Nannochloropsis gaditana Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide dereived from sequences of all known FtsH isoforms of Arabidopsis thaliana: FtsH1 Q39102 (At1g50250), FtsH2 O80860 (At2g30950), FtsH3 Q84WU8 (At2g29080), FtsH4 O80983 (At2g26140), FtsH5 Q9FH02 (At5g42270), FtsH6 Q1PDW5 (At5g15250), FtsH7 Q9SD67 (At3g47060), FtsH8 Q8W585 (At1g06430), FtsH9 Q9FIM2 (At5g58870), FtsH10 Q8VZI8 (At1g07510), FtsH11 Q9FGM0 (At5g53170) as well as 4 FtsH isoforms of Synechocystis sp. PCC6803NP_440330.1, NP_442160.1, NP_440797.1, NP_440525.1
For detection on a diatom samples, load of 5-10 µg/well is required as well as usage of enchanced chemiluminescence.The antibody also detects the chloroplast-encoded FtsH from Thalassiosira pseudonana (diatom) along with 3 related isoforms encode in the nuclear genome of Thalassiosira pseudonana.For detection in BlueNative, recommened dilution is 1: 500.
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Dogra et al. (2019). Oxidative post-translational modification of EXECUTER1 is required for singlet oxygen sensing in plastids. Nat Commun. 2019 Jun 27;10(1):2834. doi: 10.1038/s41467-019-10760-6. Li et al. (2016). A Hard Day's Night: Diatoms Continue Recycling Photosystem II in the Dark. Front. Mar. Sci., 08 November 2016Tietz et al. (2015). Functional Implications of Photosystem II Crystal Formation in Photosynthetic Membranes. J Biol Chem. 2015 Apr 20. pii: jbc.M114.619841.Campbell et al. (2013). Photosystem II protein clearance and FtsH function in the diatom Thalassiosira pseudonana. Photosynth. Res. March 16.
One of the several classes of mitochondrial proteases is membrane bound, ATPdependent FtsH protease. Their function is very important for the control of protein quality and quantity by degradation of unassembled subunits. Other names: AtFtsH3, cell division protease ftsH homolog 3, mitochondrial, AtFtsH10, cell division protease ftsH homolog 10, mitochondrial
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide dereived from sequences of Arabidopsis thaliana FtsH3 and FtsH10 with localization to mitochondria Q84WU8, At2g29080 and Q8VZI8, At1g07510
Applications:
Blue Native PAGE (BN-PAGE/SDS-PAGE), Western blot (WB)
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Kolodziejczak et al. (2018). m-AAA Complexes Are Not Crucial for the Survival of Arabidopsis Under Optimal Growth Conditions Despite Their Importance for Mitochondrial Translation. Plant Cell Physiol. 2018 May 1;59(5):1006-1016. doi: 10.1093/pcp/pcy041.Piechota et al. (2010). Identification and characetization of high-molecular-weight complexes fromed by m-AAA proteases and prohibitins in mitochondria of Arabidopsis thaliana. J Biol Chem. 2010 Apr 23;285(17):12512-21. doi: 10.1074/jbc.M109.063644.
Special application note:
Blue-native (2D BN/SDS-PAGE) methodology is described in Piechota et al. 2010
FtsZ (cell division GTPase) is a well characterized protein of the bacterial cell division apparatus. This protein accumulates early in dividing cells, and has a crucial role during septum formation in most bacteria. It has also been accepted as the bacterial cytoskeletal counterpart to eukaryotic microtubules. Synonymes: sifB, SulB.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Vedyaykin et al. (2020). SulA is able to block cell division in Escherichia coli by a mechanism different from sequestration. Biochem Biophys Res Commun . DOI: 10.1016/j.bbrc.2020.03.012 Ranjit et al. (2020). Chlamydial MreB Directs Cell Division and Peptidoglycan Synthesis in Escherichia coli in the Absence of FtsZ Activity. mBio. 2020 Feb 18;11(1). pii: e03222-19. doi: 10.1128/mBio.03222-19. (Immunofluorescence)Sekar et al. (2018). Synthesis and degradation of FtsZ quantitatively predict the first cell division in starved bacteria. Mol Syst Biol. 2018 Nov 5;14(11):e8623. doi: 10.15252/msb.20188623.Mückl et al. (2018). Filamentation and restoration of normal growth in Escherichia coli using a combined CRISPRi sgRNA/antisense RNA approach. PLoS One. 2018 Sep 11;13(9):e0198058. doi: 10.1371/journal.pone.0198058. eCollection 2018.Pende et al. (2014). Size-independent symmetric division in extraordinarily long cells. Nat Commun. 2014 Sep 15;5:4803. doi: 10.1038/ncomms5803.Söderström et al. (2014). Disassembly of the divisome in Escherichia coli: Evidence that FtsZ dissociates before compartmentalisation. Mol Microbiol. 2014 Feb 7. doi: 10.1111/mmi.12534. (western blot and immunofluorescence)
FtsZ (cell division GTPase) is a well characterized protein of the bacterial cell division apparatus. This protein accumulates early in dividing cells, and has a crucial role during septum formation in most bacteria as well as in chloroplasts. It has also been accepted as the bacterial cytoskeletal counterpart to eukaryotic microtubules.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
This antibody can be used as a loading control antibody in cyanobacteria. Immunofluorescence has been done by labelling Synechococcus elongatus cells at 30°C for 2 hours with FtsZ antibodies diluted to 1: 500 in blocking buffer. Detection images can be found in Kabeya et al (2010).This product can be sold containing ProClin if requested.
Kurmayer et al. (2020). Chemically labeled toxins or bioactive peptides show a heterogeneous intracellular distribution and low spatial overlap with autofluorescence in bloom-forming cyanobacteria. Sci Rep. 2020 Feb 17;10(1):2781. doi: 10.1038/s41598-020-59381-w. (Immunofluorescence)Zhan et al. (2018). Photobleaching Enables Super-resolution Imaging of the FtsZ Ring in the Cyanobacterium Prochlorococcus. J Vis Exp. 2018 Nov 6;(141). doi: 10.3791/58603.MacCready et al. (2016). Robust Min-System Oscillation in the Presence of Internal Photosynthetic Membranes in Cyanobacteria. Molecular Microbiology November 5 2016. doi: 10.1111/mmi.13571Probst et al. (2014). Biology of a widespread uncultivated archaeon that contributes to carbon fixation in the subsurface. Nat Commun. 2014 Nov 26;5:5497. doi: 10.1038/ncomms6497.Miyagishima et al. (2014). DipM is required for peptidoglycan hydrolysis during chloroplast division. BMC Plant Biol. 2014 Mar 6;14(1):57. (immunofluorescence)Plominsky et al. (2013). Dinitrogen Fixation Is Restricted to the Terminal Heterocysts in the Invasive Cyanobacterium Cylindrospermopsis raciborskii CS-505. PLOS ONE, Open Access.Kabeya et al (2010). The YlmG protein has a conserved function related to the distribution of nucleoids in chloroplasts and cyanobacteria. BMC Plant Biology 10:57.
Special application note:
To detect E.coli FtsZ protein we recommend a following product: AS10 715 | anti-FtsZ procaryotic cell division GTPase (bacterial), rabbit antibodyTo detect FtsZ protein in higher plants following antibodies are recommended: AS09 413 | Anti-FtsZ1 and 2 | Plant cell division protein FtsZ1 and FtsZ2, rabbit antibodiesAS13 2651 | Anti-FtsZ2 | Plant cell division protein ftsZ2, rabbit antibodies
FtsZ1 (cell division protein FtsZ homolog 1) is required for plastid cell division. Localization: pollen grain and plastids of vegetative cells. Alternative names: Chloroplast FtsZ, Protein accumulation and replication of chloroplasts 10, Protein plastid movement impaired4.FtsZ2 (cell visision protein FtsZ homolog 2) is required for plastid cell division. Present in two isoforms: FtsZ2-1 and FtsZ2-2. Alternative names: Plastid division protein FTSZ2.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Hordeum vulgare
Expected Species:
Chlamydomons reinhardtii, Cucumis sativus, Gentiana lutea, Glycine max, Gossypium arobretum, Jatropha manihot, Lilium longiflorum, Lupinus angustifolius, Manihot esculenta, Marchantia aquatica, Medicago truncatula, Morus notabilis, Nannochloropsis gaditana, Nicotiana tabacum, Oryza sativa, Physcomitrella patens, populus trichocarpa, Ricinus communis, Solanum lycopersicum, Sorgum bicolor, Theobroma cacao, Triticum uRatum, Zea mays, Yellow gentian, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
Recombinant part of Arabidopsis thalina FtsZ conserved in FtsZ1 Q42545 At5g55280 and FtsZ2 including FtsZ2-1 O82533, At2g36250 and FtsZ2-2 Q9LXJ0, At3g52750 and in a wide range of FtsZ proteins from other plant species.
FtsZ2 belongs to FtsZ family and binds to FtsZ1 and exhibits GTPase activity. Component of a plastid division machinery.Alternative names: Plastid division protein FTSZ2-1, AtFtsZ2-1, Plastid division protein FTSZ2-2, AtFtsZ2-2.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Hordeum vulgare
Expected Species:
Glycne max, Jatropha manihot, Ricinus communis Species of your interest not listed? Contact us
Immunogen:
Recombinant part of Arabidopsis thalina FtsZ2 conserved in FtsZ2-1 UniProt: O82533, TAIR: At2g36250, FtsZ2-2 UniProt: Q9LXJ0, TAIR: AT3G52750
This antibody specifically cross-reacts against fucose residues bound to the protein N-glycans in alpha 1,3. This residue is characterisitc of the plant protein N-glycans and is absent in protein N-glycans from animals. This residue is added in the Golgi apparatus.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Higher plants, Pheodactylum tricornutum (diatom)
Expected Species:
Higher plants, Phaeodactylum tricornutum
Immunogen:
core fucose residues bound to the N-glycan in alpha 1,3
Applications:
ELISA (ELISA), Immunolocalization (IL), Western blot (WB)
Controls:PLA2 (phospholipase 2 from bee venom) which contains only α1.3 fucose, Sigma, product number P9279.Type II - horseradish peroxidase which contains β1.2 Xylose and α1.3 fucose, Sigma, product number P8250.The antibody does not recognize alpha 1,6-fucose.
Application Details:
0.5 µg/ml (ELISA), 1 : 40 (IL), 1 ug/10 ml (WB)
Purity:
Affinity purified serum in PBS, pH 7.4
Reconstitution:
For reconstitution add 50 µl of sterile water.
Related products:
AS07 267 | Anti-xylose, rabbit antibodiesPlant and algal protein extraction buffer | AgriseraSuperDeal
Molecular Weight:
10 - 100 for various glycoproteins
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Zhang et al. (2018). An important role of L -fucose biosynthesis and protein fucosylation genes in Arabidopsis immunity. New Phytol. 2018 Dec 15. doi: 10.1111/nph.15639.Jansing et al. (2018). CRISPR/Cas9-mediated knockout of six glycosyltransferase genes in Nicotiana benthamiana for the production of recombinant proteins lacking β-1,2-xylose and core α-1,3-fucose. Plant Biotechnol J. 2018 Jul 3. doi: 10.1111/pbi.12981.Nakanishi et al. (2017). Protection of Human Colon Cells from Shiga Toxin by Plant-based Recombinant Secretory IgA. Sci Rep. 2017 Apr 3;7:45843. doi: 10.1038/srep45843. (ELISA)Hanania et al. (2017). Establishment of a tobacco BY2 cell line devoid of plant specific xylose and fucose as a platform for the production of biotherapeutic proteins. Plant Biotechnol J. 2017 Feb 3. doi: 10.1111/pbi.12702.Ebert et al. (2015). Identification and Characterization of a Golgi-Localized UDP-Xylose Transporter Family from Arabidopsis. Plant Cell. 2015 Mar 24. pii: tpc.114.133827.Lehtimäki et al. (2014). Posttranslational modifications of FERREDOXIN-NADP+ OXIDOREDUCTASE in Arabidopsis chloroplasts. Plant Physiol. 2014 Dec;166(4):1764-76. doi: 10.1104/pp.114.249094Baiet et all. (2010). N-glycans of Phaeodactylum tricornutum diatom and functional characterization of its N-acetylglucosaminyltransferase I enzyme. J Biol. Chem. Dec 17.
Special application note:
Alpha (1,3) fucose is present not only in plants but also in some invertebrates (such as nematodes, bees, etc.) . However, cross-reaction with glycoproteins from these organisms is weaker than the one observed in plants. This sugar residue does not exist in mammals, in their endogenous glycoproteins.
GAI is a probable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Less sensitive to GA. Synonymes:GRAS family protein 3, AtGRAS-3, Gibberelic acid-insensitive mutant protein, Restoration of growth on ammonia protein 2.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Immunogen:
KLH-conugated synthetic peptide, chosen from Arabidopsis thaliana GAI protein sequence UniProt: Q9LQT8, TAIR: At1g14920
Homogenization with thiourea and bead beater. Protocol for protein extraction from seeds can be requested here. GAI protein is present in very low levels therefore specific material should be used for analysis as well as chemiluminescence detection reagent in extreme low femtogram range, as AgriseraECLSuperBright.
Application Details:
1 : 1000 (WB) on transfected Arabidopsis thaliana protoplasts
Purity:
Affinity purified serum in PBS, pH 7.4
Reconstitution:
For reconstitution add 25 µl of sterile water.
Related products:
AS13 2638 | Anti-GID2 | F-BOX protein GID2 (SLEEPY 1), rabbit antibodiesAS11 1630 | Anti-RGA | DELLA protein RGA, rabbit antibodiesAS11 1802 | Anti-RGA-like protein | DELLA protein RGL1, rabbit antibodiesAS11 1803 | Anti-RGA-like protein 2 | DELLA protein RGL2, rabbit antibodiesPlant and algal protein extraction buffer Secondary antibodies | AgriseraSuperDeal
Gamma-glutamylcysteine synthase (gamma-ECS) (EC 6.3.2.2) catalyses the first step of glutathione (GSH) synthesis, producing gamma-glutamylcysteine (gamma-EC) from L-glutamate and cysteine. In the second step, catalyzed by GSH-S, glycine is added to the C-terminal end of (gamma-EC). GSH is the predominant non-protein thiol in all nearly where it acts as an antioxidant in stress responses and as a mobile pool of reduced sulfur. It is also important in the regulation of plant growth and development.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized in PBS pH 7.4
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Antibodies has been used in immunolocalization on Arabidopsis thaliana.
Application Details:
1 : 5000 (IL), 1 : 5000 (WB)
Purity:
Total IgG
Reconstitution:
For reconstitution add 100 µl of sterile water.
Related products:
AS11 1811 | Anti-Gamma-glutamyl-cysteine, rabbit antibodiescollection of antibodies to plant stress proteins | AgriseraSuperDeal
Molecular Weight:
50 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Shull et al. (2019). Anatase TiO2 nanoparticles induce autophagy and chloroplast degradation in thale cress (Arabidopsis thaliana). Environ Sci Technol. 2019 Jul 29. doi: 10.1021/acs.est.9b01648.Balážová et al. (2018). Zinc oxide nanoparticles phytotoxicity on halophyte from genus Salicornia. Plant Physiol Biochem. 2018 Sep;130:30-42. doi: 10.1016/j.plaphy.2018.06.013.Sobrino-Plata et al. (2014). Glutathione is a key antioxidant metabolite to cope with mercury and cadmium stress. Plant Soil, DOI 10.1007/s11104-013-2006-4.Ghanta et al. (2011). Nicotiana tabacum overexpressing γ-ECS exhibits biotic stress tolerance likely through NPR1-dependent salicylic acid-mediated pathway. Planta 233(5):895-910.
Glyceraldehyde-3-phosphate dehydrogenase is an enzyme that catalyzes the first step in glycolysis by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. This enzyme is essential for the carbohydrate metabolism and the maintenance of cellular ATP levels. Synonyme gene names: GAPC, GAPDH.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Use of this antibody as a loading control should be supported with specific experimental data.
Application Details:
1 : 1000 (WB)
Purity:
Affinity purified serum in PBS, pH 7.4
Reconstitution:
For reconstitution add 50 µl of sterile water.
Related products:
Collection of antibodies to proteins involved in carbohydrate metabolismSecondary antibodies | AgriseraSuperDeal | Loading15
Molecular Weight:
37 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Zhu et al. (2020). The RALF1-FERONIA Complex Phosphorylates eIF4E1 to Promote Protein Synthesis and Polar Root Hair Growth. Mol Plant. 2020 May 4;13(5):698-716. doi: 10.1016/j.molp.2019.12.014..
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Human, Mouse, Rabbit, Rat
Expected Species:
Dog
Immunogen:
KLH-conjugated peptide derived from the sequence of human glycogen debranching enzyme UniProt: P35573
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Sottnik et al. (2018). Elucidating the role of Agl in bladder carcinogenesis by generation and characterization of genetically engineered mice. Carcinogenesis. 2018 Nov 7. doi: 10.1093/carcin/bgy139.Richmond et al. (2018). Glycogen debranching enzyme (AGL) is a novel regulator of non-small cell lung cancer growth. Oncotarget, 2018, Vol. 9, (No. 24), pp: 16718-16730.Oldenburg et al. (2016). CD44 and RHAMM are essential for rapid growth of bladder cancer driven by loss of Glycogen Debranching Enzyme (AGL). BMC Cancer. 2016 Sep 5;16:713. doi: 10.1186/s12885-016-2756-5.Pagliarani et al. (2014). Glycogen storage disease type III: A novel Agl knockout mouse model. Biochim Biophys Acta. 2014 Aug 1. pii: S0925-4439(14)00250-6. doi: 10.1016/j.bbadis.2014.07.029.
Special application note:
This antibody can detect GDE in crude tissue homogenate in both, liver and muscle tissue and is able to immunoprecipiate GDE.Antibody will work as an excellent marker for glycogen. In tissues with no glycogen extression there will be no signal using this antibody.
GDP-L-Galactose Phosphorylase is a histidine triad (HIT) enzyme of the Smirnoff-Wheeler pathway of ascorbic acid synthesis in plants. Encoded by VTC2 gene. The enzyme catalyzes the conversion of GDP-L-galactose to L-galactose 1-phosphate in a reaction that consumes inorganic phosphate and produces GDP.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Brassica oleracea var. italica, Brassica rapa var. komatsuna, Citrus limon, Nicotiana tabacum, Spinacia oleracea, Zea mays, Chlamydomonas reinhardtii
Expected Species:
Sorghum bicolor, Oryza sativa, Physcomitrella patens Species of your interest not listed? Contact us
Immunogen:
KLH-conjuated synthetic peptide derived from known GDP-L-Galactose Phosphorylase sequences, including Arabidopsis thaliana Q8LKQ7 (At4g26850) and Chlamydomonas reinhardtii
GI (GIGANTEA) is involved in several developmental processes including regulation of circadian rhythm and photoperiodic flowering, phytochrome B signaling, circadian clock, carbohydrate metabolism and cold stress response.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized in PBS pH 7.4
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica campestris, Chrysanthemum morifolium, Dimocarpus longan, Festuca patensis, Gentiana triflora, Glycine soja, Hordeum vulgare, Liriodendron tulipifera, Lolium perenne, Lotus japonicus, Medicago truncatula, Plantago major, Populus balsamifera, Prunus dulcius, Ricinus communis, Secale cereale, Theobroma cacao, Triticum aestivum Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from protein sequence of Arabidopsis thaliana GI, UniProt:Q9SQI2,TAIR: AT1G22770
GID2 | F-BOX protein GID2 (SLEEPY 1) is an essential component of the SCF-type E3 ligase complex, which positively regulates the gibberellin signaling pathway. It mediates the ubiquitination and subsequent degradation of DELLA proteins (GAI, RGA and RGL2). Localized in nucleus and expressed in all tissues tested so far.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized in PBS pH 7.4
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica napus, Medicago truncatula, Glycine max, Ricinus communis, Populus trichocarpa, Vitis vinifera Species of your interest not listed? Contact us
Galactono-1,4-lactone dehydrogenase (GLDH) is the enzyme which catalyses last step of ascorbic acid (AA) synthesis in the mitochondria of plant cells. Alternative name: GaLDH
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized in PBS pH 7.4
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Mitochondrial, membrane or meristematic fractions were shown to be richer in GLDH expression
Application Details:
1 : 5000 (WB)
Purity:
Total IgG
Reconstitution:
For reconstitution add 100 µl of sterile water.
Related products:
Collection of antibodies to stress proteinsCollection of antibodies to mitochondrial proteins | AgriseraSuperDeal
Molecular Weight:
68 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Chen et al. (2019). Composition of Mitochondrial Complex I during the Critical Node of Seed Aging in Oryza sativa. Journal of Plant Physiology Volume 236, May 2019, Pages 7-14.Schimmeyer et al. (2016). L-Galactono-1,4-lactone dehydrogenase is an assembly factor of the membrane arm of mitochondrial complex I in Arabidopsis. Plant Mol Biol. 2016 Jan;90(1-2):117-26. doi: 10.1007/s11103-015-0400-4. Epub 2015 Oct 31.Ostaszewska-Bugajska et al. (2016). Changes in the OXPHOS system in leaf and root mitochondria of Arabidopsis thaliana subjected to long-term sulphur deficiency. Acta Physiologiae Plantarum 38:141.Bartoli et al. (2005). Ascorbate content in wheat leaves is not determined by maximal L-galactono-1, 4-lactone dehydrogenase (GalLDH) activity under drought stress. Plant Cell Environ 28:1073-1081.
GLDP (Glycine decarboxylase P protein) catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofacto. Is involved in response to cadmium ion. Alternative names: AtGLDP1, AtGLDP2, Glycine dehydrogenase (aminomethyl-transferring) 1, Glycine dehydrogenase (aminomethyl-transferring) 2, Glycine cleavage system P protein 1, Glycine cleavage system P protein 2, Glycine decarboxylase 1, Glycine decarboxylase 2, Glycine decarboxylase P-protein 1, Glycine decarboxylase P-protein 2.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
So far only leaf tissue has been used for immunolocalization and western blot experiments.
Application Details:
1:50 (IL), 1 : 5000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 µl, of sterile water.
Related products:
AS05 074 | Anti-GDC-H | H protein of glycine decarboxylase complex (GDC), rabbit antibodiesAS06 181 | Anti-GR | Glutathione reductase, rabbit antibodiescollection of antibodies to other mitochondrial proteinscollection of antibodies to proteins involved in a heavy metal stress response | AgriseraSuperDeal
Molecular Weight:
113 kDa
Not reactive in:
Tecticornia spp., Salsola soda
Selected references:
Khoshravesh et al. (2020). The Evolutionary Origin of C4 Photosynthesis in the Grass Subtribe Neurachninae.Plant Physiol. 2020 Jan;182(1):566-583. doi: 10.1104/pp.19.00925.
Glutamine synthetase (GLN or GS) is one of the key enzymes involved in nitrogen metabolism of plants. It catalyses the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. There are two general classes of glutamine synthetase in plants: GLN1, a cytosolic form and GLN2, a chloroplastic form. GLN1 is highly abundant in the vascular elements of roots nodules, flowers and fruits, functioning in the assimilation of ammonium and the biosynthesis of glutamine for nitrogen transport. GLN2 is encoded by a single gene and is highly abundant in leaf mesophyll chloroplasts. Here GLN functions in the assimilation of ammonia produced from photorespiration and the reduction of nitrate in the chloroplasts
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
KLH-conjugated synthetic peptide derived from a wide range of available sequences including all isoforms of Arabidopsis thaliana GLN1-1,1-2,1-3 and 1-4, (At5g37600, At1g66200, At3g17820, At5g16570)
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Silva et al. (2019). Characterization of plant glutamine synthetase S-nitrosation. Nitric Oxide. 2019 Apr 23;88:73-86. doi: 10.1016/j.niox.2019.04.006.Wang et al. (2018). Response of Gracilaria lemaneiformis to nitrogen deprivation. Algal Research Volume 34, September 2018, Pages 82-96.Witzel et al. (2017). Temporal impact of the vascular wilt pathogen Verticillium dahliae on tomato root proteome. J Proteomics. 2017 Oct 3;169:215-224. doi: 10.1016/j.jprot.2017.04.008.Silva et al. (2015). Possible role of glutamine synthetase of the prokaryotic type (GSI-like) in nitrogen signaling in Medicago truncatula. Volume 240, November 2015, Pages 98–108.Podgórska et al. (2013). Long-term ammonium nutrition of Arabidopsis increases the extrachloroplastic NAD(P)H/NAD(P)+ ratio and mitochondrial reactive oxygen species level in leaves but does not impair photosynthetic capacity. Plant Cell Environ. April 10.Brouwer et al. (2011) TheImpact ofLightIntensity onShade-InducedLeaf Senescence. Plant Cell Environ. Dec. 15 (ahead of print).Lang et al. (2011).Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as the basis for sub-cellular proteomics. Plant Cell Rep. Feb;30(2):205-15. (reactivity confirmed for Physcomitrella patens).
Special application note:
The antibody will recognize both, cytoplasmic and chloroplastic forms of the GS enzyme.
Glutamine synthetase (GLN or GS) is one of the key enzymes involved in nitrogen metabolism of plants. It catalyses the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. There are two general classes of glutamine synthetase in plants: GLN1, a cytosolic form and GLN2, a chloroplastic form. GLN2 is encoded by a single gene and is highly abundant in mesophyll cells of leaves for the assimilation of ammonia produced from photorespiration and the reduction of nitrate in the chloroplasts. GLN2 is a target for thioredoxin.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Brassica napus, Glycine max, Hordeum vulgare, Medicago truncatula, Pinus sylvestris, Phaseolus vulgaris, Physcomitrella patens, Populus sp., Triticum, aestivum, Zea mays Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide which is a part of part of the glutamine synthetase/guanido kinase superfamily catalytic region chosen from various available sequences, including Arabidopsis thaliana GLN2, UniProt: Q43127, TAIR: AT5G35630
Chen et al. (2018). TIC236 links the outer and inner membrane translocons of the chloroplast. Nature. 2018 Dec;564(7734):125-129. doi: 10.1038/s41586-018-0713-y.Dixit (2015). Sulfur alleviates arsenic toxicity by reducing its accumulation and modulating proteome, amino acids and thiol metabolism in rice leaves. Sci Rep. 2015 Nov 10;5:16205. doi: 10.1038/srep16205.Lee et al. (2013). Stromal protein degradation is incomplete in Arabidopsis thaliana autophagy mutants undergoing natural senescence. BMC Res Notes, Jan 17.Hu and Li (2012). The amino-terminal domain of chloroplast Hsp93 is important for its membrane association and functions in vivo. Plant Physiol. Apr;158(4):1656-65. doi: 10.1104/pp.112.193300. Epub 2012 Feb 21.
Special application note:
This product can be sold contacining proclin if requested
GluTR (glutamyl tRNA reductase) belongs to a family of oxidoreductases and is involved in porphyrin and chlorophyll biosynthesis. This enzyme class is called L-glutamate-semialdehyde: NADP+ oxidoreductase (L-glutamyl-tRNAGlu-forming).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Plant and algal protein extraction bufferSecondary antibodies | AgriseraSuperDeal
Molecular Weight:
58 | kDa (Arabidopsis thaliana)
Not reactive in:
Cyanobacteria
Selected references:
Agrawal et al. (2020). The Functions of Chloroplast Glutamyl-tRNA in Translation and Tetrapyrrole Biosynthesis. Plant Physiol. 2020 Feb 18. pii: pp.00009.2020. doi: 10.1104/pp.20.00009Montandon et al. (2019). In vivo trapping of proteins interacting with the chloroplast CLPC1 chaperone; potential substrates and adaptors. J Proteome Res. 2019 May 9. doi: 10.1021/acs.jproteome.9b00112.Nishimura et al. (2013). ClpS1 Is a Conserved Substrate Selector for the Chloroplast Clp Protease System in Arabidopsis. The Plant Cell June 2013.
Special application note:
Antibody reacts with recombinant GluTR isoforms: AtGluTR1, AtGluTR2 and NtGluTR1 (At - Arabidopsis thaliana, Nt - Nicotiana tabacum).
Glutamine oxoglutarate aminotransferase (abbreviated as GOGAT) is an enzyme involved in synthesis of glutamate from glutamine and alpha-ketoglutarate. GOGAT has two forms in plants: ferredoxin-dependent GOGAT (Fd-GOGAT) and NADH-dependent GOGAT (NADH-GOGAT). 95% of GOGAT found in plants is the Fd-GOGAT type. Fd-GOGAT is encoded by two genes, glu1 and glu2 found on chromosomes 5 and 2 respectively (in Arabidopsis). Fd-GOGAT (both forms) is highly conserved among plants, red algae, and cyanobacteria.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
KLH-conjugated synthetic peptide well conserved in known GOGAT sequences from different species including Arabidopsis thaliana Fd-GOGAT 1 Q9ZNZ7, At5g04140 and Fd-GOGAT 2 Q9T0P4, At2g41220
This antibody can be used as a plastidial marker for leaf material, not roots where levels of GOGAT are too low. A 40 kDa band present in A. thaliana sample is not competed away during antibody neutralization assay. In this assay free peptide used for antibody production is incubated together with anti-GOGAT antibodies. Due to the MW of this protein we suggest to use a gradient gel for protein separation and a longer transfer time.
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 µl of sterile water.
Related products:
Collection of antibodies to proteins involved in nitrogen metabolismPlant and algal protein extraction bufferSecondary antibodies | AgriseraSuperDeal
Molecular Weight:
177 | 170-180 kDa depending upon the species
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Touraine et al. (2019). Iron-sulfur protein NFU2 is required for branched-chain amino acid synthesis in Arabidopsis roots. J Exp Bot. 2019 Mar 27;70(6):1875-1889. doi: 10.1093/jxb/erz050.Wang et al. (2018). Genetic variations in ARE1 mediate grain yield by modulating nitrogen utilization in rice. Nat Commun. 2018 Feb 21;9(1):735. doi: 10.1038/s41467-017-02781-w.Nath et al. (2016). A Nitrogen-Fixing Subunit Essential for Accumulating 4Fe-4S-Containing Photosystem I Core Proteins. Plant Physiol. 2016 Dec;172(4):2459-2470. Epub 2016 Oct 26.Jayawardena et al. (2016). Elevated CO2 plus chronic warming reduces nitrogen uptake and levels or activities of nitrogen -uptake and -assimilatory proteins in tomato roots. Physiol Plant. 2016 Nov 28. doi: 10.1111/ppl.12532. [Epub ahead of print]Takabayashi et al. (2016) Direct interaction with ACR11 is necessary for post-transcriptional control of GLU1-encoded ferredoxin-dependent glutamate synthase in leaves. Sci Rep. 2016 Jul 14;6:29668. doi: 10.1038/srep29668.Yang et al. (2016). Rice Ferredoxin-Dependent Glutamate Synthase Regulates Nitrogen-Carbon Metabolomes and Is Genetically Differentiated between japonica and indica Subspecies. Mol Plant. 2016 Sep 24. pii: S1674-2052(16)30195-2. doi: 10.1016/j.molp.2016.09.004.Moscatelli et al. (2015). Characterisation of detergent-insoluble membranes in pollen tubes of Nicotiana tabacum (L.). Biol Open. 2015 Feb 20. pii: BIO201410249. doi: 10.1242/bio.201410249.Podgórska et al. (2013). Long-term ammonium nutrition of Arabidopsis increases the extrachloroplastic NAD(P)H/NAD(P)+ ratio and mitochondrial reactive oxygen species level in leaves but does not impair photosynthetic capacity. Plant Cell Environ. April 10.
Glycolate oxidase (GOX) is an enzyme which modulates reactive oxygen species-mediated signal transduction during nonhost resistance. There are three GOX isoforms in Arabidopsis thaliana: GOX1,2,3. Alternative name: Peroxisomal (S)-2-hydroxy-acid oxidase GLO.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Lupinus albus, Oryza sativa
Expected Species:
Glycine max, Hordeum vulgare, Medicago truncatula, Nicotiana tabacum, Phaseolus vulgaris, Solanum lycopersicum, Solanum tuberosum, Spinacia oleracea, Triticum sp. Zea mays, Vitis viniferaSpecies of your interest not listed? Contact us
collection of antibodies to proteins involved in pathogen attackAlgal protein extraction buffer | AgriseraSuperDeal
Not reactive in:
Gracilaria lemaneiformis
Selected references:
Umnajkitikorn et al. (2020). Silencing of OsCV (chloroplast vesiculation) maintained photorespiration and N assimilation in rice plants grown under elevated CO2. Plant Cell Environ . 2020 Apr;43(4):920-933. doi: 10.1111/pce.13723.
Glycogen phosphorylase (EC 2.4.1.1) is an enzyme which is catalyzing the rate limiting step in the degradation of glycogen in animals by releasing glucose-1-phosphate from the terminal alpha1,4-glycosidic bond.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Samples used to test this antibody were: muscle and liver homogenates, purified glycogen. This antiboy will recogize glycogen phosphorylase from both liver and muscle. It has been used in ICC on human primary myotubes.
