The NEXTflex RNA-Seq Barcodes Kits contain 6, 12, 24 or 48 unique barcodes, enabling the user to multiplex up to 48 samples per flow cell lane. These kits ship on dry ice. These barcodes can be used with RNA-Seq kits, directional RNA-Seq kits, Rapid RNA-Seq kits and the Rapid Directional RNA-Seq kits.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
The NEXTflex RNA-Seq 2.0 UDI Barcodes are adapters designed for use with the NEXTflex Rapid Directional RNA-seq Kit 2.0.
The NEXTFLEX® Unique Dual Index Barcodes utilize two unique 8 nt indexes on each UDI adapter. The 8 nt indices are designed with a Hamming Distance of at least three throughout the entire series, permitting error correction. Each NEXTFLEX® Unique Dual Index Barcode comprises the entire flow cell binding site, sequencing primer binding site, and index sequences, making PCR enrichment optional.
Patterned flow cells, such as those found on the NovaSeq® and HiSeq® 3000/4000 platforms, have been recognized to suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices during sequencing prevents such mis-assigned reads from appearing in final data sets, allowing for the highest assurance of data integrity.
Barcode sets available: NOVA-512920-eval16 NEXTflex RNA-Seq 2.0 UDI barcodes (Barcodes 1-8 in tubes) NOVA-512920-eval48A NEXTflex RNA-Seq 2.0 UDI barcodes (Barcodes 1-24 in tubes) NOVA-512920-eval48B NEXTflex RNA-Seq 2.0 UDI barcodes (Barcodes 24-48 in tubes) NOVA-512920 NEXTflex RNA-Seq 2.0 UDI barcodes (Barcodes 1-96 in a plate) with custom sets available up to 1920 barcoded adapters.
BENEFITS:- >>> Unique dual index adapters for multiplexing up to 384 Illumina® sequencing libraries >>> Mitigate index hopping or spread of signal that can occur during sequencing >>> Each lot of the NEXTFLEX® Unique Dual Index Barcodes is functionally validated and tested for index purity by sequencing >>> Compatible with both paired-end and single-read Illumina® sequencing
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 512914
The NEXTflex RNA-Seq Barcodes Kits contain 6, 12, 24 or 48 unique barcodes, enabling the user to multiplex up to 48 samples per flow cell lane. These kits ship on dry ice. These barcodes can be used with RNA-Seq kits, directional RNA-Seq kits, Rapid RNA-Seq kits and the Rapid Directional RNA-Seq kits.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
The NEXTflex RNA-Seq 2.0 UDI Barcodes are adapters designed for use with the NEXTflex Rapid Directional RNA-seq Kit 2.0.
The NEXTFLEX® Unique Dual Index Barcodes utilize two unique 8 nt indexes on each UDI adapter. The 8 nt indices are designed with a Hamming Distance of at least three throughout the entire series, permitting error correction. Each NEXTFLEX® Unique Dual Index Barcode comprises the entire flow cell binding site, sequencing primer binding site, and index sequences, making PCR enrichment optional.
Patterned flow cells, such as those found on the NovaSeq® and HiSeq® 3000/4000 platforms, have been recognized to suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices during sequencing prevents such mis-assigned reads from appearing in final data sets, allowing for the highest assurance of data integrity.
Barcode sets available: NOVA-512920-eval16 NEXTflex RNA-Seq 2.0 UDI barcodes (Barcodes 1-8 in tubes) NOVA-512920-eval48A NEXTflex RNA-Seq 2.0 UDI barcodes (Barcodes 1-24 in tubes) NOVA-512920-eval48B NEXTflex RNA-Seq 2.0 UDI barcodes (Barcodes 24-48 in tubes) NOVA-512920 NEXTflex RNA-Seq 2.0 UDI barcodes (Barcodes 1-96 in a plate) with custom sets available up to 1920 barcoded adapters.
BENEFITS:- >>> Unique dual index adapters for multiplexing up to 384 Illumina® sequencing libraries >>> Mitigate index hopping or spread of signal that can occur during sequencing >>> Each lot of the NEXTFLEX® Unique Dual Index Barcodes is functionally validated and tested for index purity by sequencing >>> Compatible with both paired-end and single-read Illumina® sequencing
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 512913
The NEXTflex RNA-Seq Barcodes Kits contain 6, 12, 24 or 48 unique barcodes, enabling the user to multiplex up to 48 samples per flow cell lane. These kits ship on dry ice. These barcodes can be used with RNA-Seq kits, directional RNA-Seq kits, Rapid RNA-Seq kits and the Rapid Directional RNA-Seq kits.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
The NEXTflex RNA-Seq 2.0 UDI Barcodes are adapters designed for use with the NEXTflex Rapid Directional RNA-seq Kit 2.0.
The NEXTFLEX® Unique Dual Index Barcodes utilize two unique 8 nt indexes on each UDI adapter. The 8 nt indices are designed with a Hamming Distance of at least three throughout the entire series, permitting error correction. Each NEXTFLEX® Unique Dual Index Barcode comprises the entire flow cell binding site, sequencing primer binding site, and index sequences, making PCR enrichment optional.
Patterned flow cells, such as those found on the NovaSeq® and HiSeq® 3000/4000 platforms, have been recognized to suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices during sequencing prevents such mis-assigned reads from appearing in final data sets, allowing for the highest assurance of data integrity.
Barcode sets available: NOVA-512920-eval16 NEXTflex RNA-Seq 2.0 UDI barcodes (Barcodes 1-8 in tubes) NOVA-512920-eval48A NEXTflex RNA-Seq 2.0 UDI barcodes (Barcodes 1-24 in tubes) NOVA-512920-eval48B NEXTflex RNA-Seq 2.0 UDI barcodes (Barcodes 24-48 in tubes) NOVA-512920 NEXTflex RNA-Seq 2.0 UDI barcodes (Barcodes 1-96 in a plate) with custom sets available up to 1920 barcoded adapters.
BENEFITS:- >>> Unique dual index adapters for multiplexing up to 384 Illumina® sequencing libraries >>> Mitigate index hopping or spread of signal that can occur during sequencing >>> Each lot of the NEXTFLEX® Unique Dual Index Barcodes is functionally validated and tested for index purity by sequencing >>> Compatible with both paired-end and single-read Illumina® sequencing
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 512914
The NEXTflex RNA-Seq Barcodes Kits contain 6, 12, 24 or 48 unique barcodes, enabling the user to multiplex up to 48 samples per flow cell lane. These kits ship on dry ice. These barcodes can be used with RNA-Seq kits, directional RNA-Seq kits, Rapid RNA-Seq kits and the Rapid Directional RNA-Seq kits.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
The NEXTflex RNA-Seq 2.0 UDI Barcodes are adapters designed for use with the NEXTflex Rapid Directional RNA-seq Kit 2.0.
The NEXTFLEX® Unique Dual Index Barcodes utilize two unique 8 nt indexes on each UDI adapter. The 8 nt indices are designed with a Hamming Distance of at least three throughout the entire series, permitting error correction. Each NEXTFLEX® Unique Dual Index Barcode comprises the entire flow cell binding site, sequencing primer binding site, and index sequences, making PCR enrichment optional.
Patterned flow cells, such as those found on the NovaSeq® and HiSeq® 3000/4000 platforms, have been recognized to suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices during sequencing prevents such mis-assigned reads from appearing in final data sets, allowing for the highest assurance of data integrity.
Barcode sets available: NOVA-512920-eval16 NEXTflex RNA-Seq 2.0 UDI barcodes (Barcodes 1-8 in tubes) NOVA-512920-eval48A NEXTflex RNA-Seq 2.0 UDI barcodes (Barcodes 1-24 in tubes) NOVA-512920-eval48B NEXTflex RNA-Seq 2.0 UDI barcodes (Barcodes 24-48 in tubes) NOVA-512920 NEXTflex RNA-Seq 2.0 UDI barcodes (Barcodes 1-96 in a plate) with custom sets available up to 1920 barcoded adapters.
BENEFITS:- >>> Unique dual index adapters for multiplexing up to 384 Illumina® sequencing libraries >>> Mitigate index hopping or spread of signal that can occur during sequencing >>> Each lot of the NEXTFLEX® Unique Dual Index Barcodes is functionally validated and tested for index purity by sequencing >>> Compatible with both paired-end and single-read Illumina® sequencing
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 512914
The NEXTflex Poly(A) Beads (magnetic separation beads) provide a convenient method for batch purification of pure, intact mRNA upstream of library preparation for RNA-Seq. NEXTflex Poly(A) Beads beads use oligo(dT) primer to isolate polyadenylated messenger RNAs from 1 µg 10 µg of previously isolated total RNA. Separation using a magnetic stand allows for high mRNA recovery. The intact mRNA is eluted in small volumes eliminating the need for precipitation. Ease of use and consistent results makes the NEXTflex Poly(A) Beads ideal for NGS library preparation applications.
These beads have been validated with the following kits:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
[BENEFITS]: >>> 10 ng - 5 ug total starting RNA >>> Conserve resources by selecting for mRNA prior to library prep >>> Magnetic bead-based protocol for easier wash step and higher recovery >>> No organic solvents or precipitation step required >>> Validated for NGS library preparation
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 512979
The NEXTflex Poly(A) Beads (magnetic separation beads) provide a convenient method for batch purification of pure, intact mRNA upstream of library preparation for RNA-Seq. NEXTflex Poly(A) Beads beads use oligo(dT) primer to isolate polyadenylated messenger RNAs from 1 µg 10 µg of previously isolated total RNA. Separation using a magnetic stand allows for high mRNA recovery. The intact mRNA is eluted in small volumes eliminating the need for precipitation. Ease of use and consistent results makes the NEXTflex Poly(A) Beads ideal for NGS library preparation applications.
These beads have been validated with the following kits:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
[BENEFITS]: >>> 10 ng - 5 ug total starting RNA >>> Conserve resources by selecting for mRNA prior to library prep >>> Magnetic bead-based protocol for easier wash step and higher recovery >>> No organic solvents or precipitation step required >>> Validated for NGS library preparation
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 512980
The NEXTflex Poly(A) Beads (magnetic separation beads) provide a convenient method for batch purification of pure, intact mRNA upstream of library preparation for RNA-Seq. NEXTflex Poly(A) Beads beads use oligo(dT) primer to isolate polyadenylated messenger RNAs from 1 µg 10 µg of previously isolated total RNA. Separation using a magnetic stand allows for high mRNA recovery. The intact mRNA is eluted in small volumes eliminating the need for precipitation. Ease of use and consistent results makes the NEXTflex Poly(A) Beads ideal for NGS library preparation applications.
These beads have been validated with the following kits:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
[BENEFITS]: >>> 10 ng - 5 ug total starting RNA >>> Conserve resources by selecting for mRNA prior to library prep >>> Magnetic bead-based protocol for easier wash step and higher recovery >>> No organic solvents or precipitation step required >>> Validated for NGS library preparation
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 512981
Several approaches have been used to remove ribosomal RNA (rRNA) from total RNA samples intended for use to produce libraries for NGS RNA-Seq experiments. Removal of rRNA avoids the waste of reagents and bioinformatic resources to sequence and align the ~85% of total RNA comprised of rRNA, which is generally not a target of interest in NGS sequencing experiments.
The NEXTFLEX® Poly(A) Beads 2.0 offer improved mRNA yields, lower rRNA contamination, and a more simplified protocol, verified from 10 ng - 5 µg of total RNA for a convenient method for the purification of pure, intact mRNA upstream of RNA-Seq library prep.
Poly(A)-tailed mRNA is isolated via magnetic beads conjugated to oligo(dT), and separation using a magnetic stand allows for high mRNA recovery. The intact mRNA is eluted in small volumes eliminating the need for precipitation. These beads have been validated with the new and improved NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0.
The NEXTFLEX® Poly(A) beads 2.0 are an easy and cost-effective method for removing rRNA in RNA-Seq libraries. This product takes advantage of the tract of adenosine residues present at the 3 ends of the vast majority of protein-coding mRNAs. Hybridization of these poly(A) tails to tracts of complementary thymidines (oligo dT) immobilized on solid supports allows the retrieval of poly(A)+ RNAs by recovering the solid support along with the hybridized RNA complexes.
The NEXTFLEX® Poly(A) Beads 2.0 contains enough material to extract mRNA from 8, 48 or 96 total RNA samples for subsequent RNA-seq library prep or other applications. The shelf life of all reagents is 12 months when stored properly. All components can safely be stored at 4°C.
These beads have been validated with the following kits: NEXTflex Directional RNA-Seq Kit NEXTflex Directional RNA-Seq Kit 2.0
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
[BENEFITS]: >>> 10 ng - 5 ug total starting RNA >>> Conserve resources by selecting for mRNA prior to library prep >>> Magnetic bead-based protocol for easier wash step and higher recovery >>> No organic solvents or precipitation step required >>> Validated for NGS library preparation
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Several approaches have been used to remove ribosomal RNA (rRNA) from total RNA samples intended for use to produce libraries for NGS RNA-Seq experiments. Removal of rRNA avoids the waste of reagents and bioinformatic resources to sequence and align the ~85% of total RNA comprised of rRNA, which is generally not a target of interest in NGS sequencing experiments.
The NEXTFLEX® Poly(A) Beads 2.0 offer improved mRNA yields, lower rRNA contamination, and a more simplified protocol, verified from 10 ng - 5 µg of total RNA for a convenient method for the purification of pure, intact mRNA upstream of RNA-Seq library prep.
Poly(A)-tailed mRNA is isolated via magnetic beads conjugated to oligo(dT), and separation using a magnetic stand allows for high mRNA recovery. The intact mRNA is eluted in small volumes eliminating the need for precipitation. These beads have been validated with the new and improved NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0.
The NEXTFLEX® Poly(A) beads 2.0 are an easy and cost-effective method for removing rRNA in RNA-Seq libraries. This product takes advantage of the tract of adenosine residues present at the 3 ends of the vast majority of protein-coding mRNAs. Hybridization of these poly(A) tails to tracts of complementary thymidines (oligo dT) immobilized on solid supports allows the retrieval of poly(A)+ RNAs by recovering the solid support along with the hybridized RNA complexes.
The NEXTFLEX® Poly(A) Beads 2.0 contains enough material to extract mRNA from 8, 48 or 96 total RNA samples for subsequent RNA-seq library prep or other applications. The shelf life of all reagents is 12 months when stored properly. All components can safely be stored at 4°C.
These beads have been validated with the following kits: NEXTflex Directional RNA-Seq Kit NEXTflex Directional RNA-Seq Kit 2.0
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
[BENEFITS]: >>> 10 ng - 5 ug total starting RNA >>> Conserve resources by selecting for mRNA prior to library prep >>> Magnetic bead-based protocol for easier wash step and higher recovery >>> No organic solvents or precipitation step required >>> Validated for NGS library preparation
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Several approaches have been used to remove ribosomal RNA (rRNA) from total RNA samples intended for use to produce libraries for NGS RNA-Seq experiments. Removal of rRNA avoids the waste of reagents and bioinformatic resources to sequence and align the ~85% of total RNA comprised of rRNA, which is generally not a target of interest in NGS sequencing experiments.
The NEXTFLEX® Poly(A) Beads 2.0 offer improved mRNA yields, lower rRNA contamination, and a more simplified protocol, verified from 10 ng - 5 µg of total RNA for a convenient method for the purification of pure, intact mRNA upstream of RNA-Seq library prep.
Poly(A)-tailed mRNA is isolated via magnetic beads conjugated to oligo(dT), and separation using a magnetic stand allows for high mRNA recovery. The intact mRNA is eluted in small volumes eliminating the need for precipitation. These beads have been validated with the new and improved NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0.
The NEXTFLEX® Poly(A) beads 2.0 are an easy and cost-effective method for removing rRNA in RNA-Seq libraries. This product takes advantage of the tract of adenosine residues present at the 3 ends of the vast majority of protein-coding mRNAs. Hybridization of these poly(A) tails to tracts of complementary thymidines (oligo dT) immobilized on solid supports allows the retrieval of poly(A)+ RNAs by recovering the solid support along with the hybridized RNA complexes.
The NEXTFLEX® Poly(A) Beads 2.0 contains enough material to extract mRNA from 8, 48 or 96 total RNA samples for subsequent RNA-seq library prep or other applications. The shelf life of all reagents is 12 months when stored properly. All components can safely be stored at 4°C.
These beads have been validated with the following kits: NEXTflex Directional RNA-Seq Kit NEXTflex Directional RNA-Seq Kit 2.0
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
[BENEFITS]: >>> 10 ng - 5 ug total starting RNA >>> Conserve resources by selecting for mRNA prior to library prep >>> Magnetic bead-based protocol for easier wash step and higher recovery >>> No organic solvents or precipitation step required >>> Validated for NGS library preparation
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
The patent pending, NEXTflex qRNA-Seq Kit, enables high precision gene expression analysis by RNA-Seq. Developed in conjunction with Cellular Research Inc., this new kit efficiently generates libraries equivalent to conventional RNA-Seq libraries, but with the added feature of molecular indexing. Similar to conventional RNA-Seq, sample RNA is converted to cDNA fragments. But prior to any PCR amplification steps, all DNA fragment ends are ligated to a pair of adapters chosen at random from a total set of 9,216 molecular indices. Individual DNA molecules of identical sequence become distinct through indexing, allowing for differentiation between re-sampling of the same molecule and sampling of a different molecule of identical sequence. Analysis using molecular indexing information provides an absolute, digital measurement of gene expression levels, irrespective of common amplification distortions observed in many RNA-Seq experiments.
[STOCHASTIC LABELING OF INDIVIDUAL DNA MOLECULES] The NEXTflex qRNA-Seq Kit contains two sets of 96 distinct molecular labels on the sequencing adapters. Each label or index consists of an 8 nucleotide barcode tag. In the ligation reaction, these 96 adapters are present in vast molar excess over the concentration of the cDNA fragments, and therefore serve as a non-depleting reservoir of molecular labels. Each end of a cDNA fragment independently and randomly chooses and ligates to a single label from this pool of 96 adapters to result in a total of 96 x 96 = 9,216 possible combinations across both ends. For every clone sequenced, paired-end reads reveals the chosen label on each end along with adjoining cDNA sequence. In addition to encoding DNA fragments at the molecular level, the kit also allows for the application of sample-specific barcodes during the library preparation PCR step. A more detailed description of the use of molecular indexing for RNA-Seq is available in our product application note.
[PROTOCOL] Using the NEXTflex qRNA-Seq Kit, mRNA or rRNA-depleted RNA is fragmented using a cationic buffer. Fragmented RNA undergoes first and second strand synthesis, followed by end-repair, adenylation, ligation and PCR.
[MULTIPLEXING] NEXTflex qRNA-Seq Kits contain barcodes so additional barcodes do not need to be purchased. The 8 rxn kit includes 4 barcodes for multiplexing. Four different versions of the 48 reaction kit are available, each contain 24 unique barcodes. Up to 96 samples can be multiplexed together.
For more information read our recent white paper written (Click the DataSheet link) in collaboration with Cellular Research, which demonstrates the capabilities of the NEXTflex qRNA-Seq Kit for quantitative transcriptome analysis.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
[BENEFITS] >>> Enables high precision measurement of RNA concentration >>> Enhanced Adapter Ligation Technology offers a larger number of unique sequencing reads >>> Input 10 - 100 ng of mRNA or rRNA-depleted total RNA >>> Embedded sample index barcodes for multiplexing of up to 96 samples >>> Automation-friendly workflow is compatible with liquid handlers >>> Functionally validated with Illumina sequencing platforms >>> Automation-friendly workflow is compatible with liquid handlers >>> Functionally validated with the Illumina GAIIx and HiSeq 2000 sequencing platforms
The NEXTflex qRNA-Seq Kit contains enough material to prepare 8 or 48 RNA samples for Illumina compatible paired end sequencing. The shelf life of all reagents is 6 months when stored properly. All components can safely be stored at -20°C.
For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 5130-11
The patent pending, NEXTflex qRNA-Seq Kit, enables high precision gene expression analysis by RNA-Seq. Developed in conjunction with Cellular Research Inc., this new kit efficiently generates libraries equivalent to conventional RNA-Seq libraries, but with the added feature of molecular indexing. Similar to conventional RNA-Seq, sample RNA is converted to cDNA fragments. But prior to any PCR amplification steps, all DNA fragment ends are ligated to a pair of adapters chosen at random from a total set of 9,216 molecular indices. Individual DNA molecules of identical sequence become distinct through indexing, allowing for differentiation between re-sampling of the same molecule and sampling of a different molecule of identical sequence. Analysis using molecular indexing information provides an absolute, digital measurement of gene expression levels, irrespective of common amplification distortions observed in many RNA-Seq experiments.
[STOCHASTIC LABELING OF INDIVIDUAL DNA MOLECULES] The NEXTflex qRNA-Seq Kit contains two sets of 96 distinct molecular labels on the sequencing adapters. Each label or index consists of an 8 nucleotide barcode tag. In the ligation reaction, these 96 adapters are present in vast molar excess over the concentration of the cDNA fragments, and therefore serve as a non-depleting reservoir of molecular labels. Each end of a cDNA fragment independently and randomly chooses and ligates to a single label from this pool of 96 adapters to result in a total of 96 x 96 = 9,216 possible combinations across both ends. For every clone sequenced, paired-end reads reveals the chosen label on each end along with adjoining cDNA sequence. In addition to encoding DNA fragments at the molecular level, the kit also allows for the application of sample-specific barcodes during the library preparation PCR step. A more detailed description of the use of molecular indexing for RNA-Seq is available in our product application note.
[PROTOCOL] Using the NEXTflex qRNA-Seq Kit, mRNA or rRNA-depleted RNA is fragmented using a cationic buffer. Fragmented RNA undergoes first and second strand synthesis, followed by end-repair, adenylation, ligation and PCR.
[MULTIPLEXING] NEXTflex qRNA-Seq Kits contain barcodes so additional barcodes do not need to be purchased. The 8 rxn kit includes 4 barcodes for multiplexing. Four different versions of the 48 reaction kit are available, each contain 24 unique barcodes. Up to 96 samples can be multiplexed together.
For more information read our recent white paper written (Click the DataSheet link) in collaboration with Cellular Research, which demonstrates the capabilities of the NEXTflex qRNA-Seq Kit for quantitative transcriptome analysis.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
[BENEFITS] >>> Enables high precision measurement of RNA concentration >>> Enhanced Adapter Ligation Technology offers a larger number of unique sequencing reads >>> Input 10 - 100 ng of mRNA or rRNA-depleted total RNA >>> Embedded sample index barcodes for multiplexing of up to 96 samples >>> Automation-friendly workflow is compatible with liquid handlers >>> Functionally validated with Illumina sequencing platforms >>> Automation-friendly workflow is compatible with liquid handlers >>> Functionally validated with the Illumina GAIIx and HiSeq 2000 sequencing platforms
The NEXTflex qRNA-Seq Kit contains enough material to prepare 8 or 48 RNA samples for Illumina compatible paired end sequencing. The shelf life of all reagents is 6 months when stored properly. All components can safely be stored at -20°C.
For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 5130-12
The patent pending, NEXTflex qRNA-Seq Kit, enables high precision gene expression analysis by RNA-Seq. Developed in conjunction with Cellular Research Inc., this new kit efficiently generates libraries equivalent to conventional RNA-Seq libraries, but with the added feature of molecular indexing. Similar to conventional RNA-Seq, sample RNA is converted to cDNA fragments. But prior to any PCR amplification steps, all DNA fragment ends are ligated to a pair of adapters chosen at random from a total set of 9,216 molecular indices. Individual DNA molecules of identical sequence become distinct through indexing, allowing for differentiation between re-sampling of the same molecule and sampling of a different molecule of identical sequence. Analysis using molecular indexing information provides an absolute, digital measurement of gene expression levels, irrespective of common amplification distortions observed in many RNA-Seq experiments.
[STOCHASTIC LABELING OF INDIVIDUAL DNA MOLECULES] The NEXTflex qRNA-Seq Kit contains two sets of 96 distinct molecular labels on the sequencing adapters. Each label or index consists of an 8 nucleotide barcode tag. In the ligation reaction, these 96 adapters are present in vast molar excess over the concentration of the cDNA fragments, and therefore serve as a non-depleting reservoir of molecular labels. Each end of a cDNA fragment independently and randomly chooses and ligates to a single label from this pool of 96 adapters to result in a total of 96 x 96 = 9,216 possible combinations across both ends. For every clone sequenced, paired-end reads reveals the chosen label on each end along with adjoining cDNA sequence. In addition to encoding DNA fragments at the molecular level, the kit also allows for the application of sample-specific barcodes during the library preparation PCR step. A more detailed description of the use of molecular indexing for RNA-Seq is available in our product application note.
[PROTOCOL] Using the NEXTflex qRNA-Seq Kit, mRNA or rRNA-depleted RNA is fragmented using a cationic buffer. Fragmented RNA undergoes first and second strand synthesis, followed by end-repair, adenylation, ligation and PCR.
[MULTIPLEXING] NEXTflex qRNA-Seq Kits contain barcodes so additional barcodes do not need to be purchased. The 8 rxn kit includes 4 barcodes for multiplexing. Four different versions of the 48 reaction kit are available, each contain 24 unique barcodes. Up to 96 samples can be multiplexed together.
For more information read our recent white paper written (Click the DataSheet link) in collaboration with Cellular Research, which demonstrates the capabilities of the NEXTflex qRNA-Seq Kit for quantitative transcriptome analysis.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
[BENEFITS] >>> Enables high precision measurement of RNA concentration >>> Enhanced Adapter Ligation Technology offers a larger number of unique sequencing reads >>> Input 10 - 100 ng of mRNA or rRNA-depleted total RNA >>> Embedded sample index barcodes for multiplexing of up to 96 samples >>> Automation-friendly workflow is compatible with liquid handlers >>> Functionally validated with Illumina sequencing platforms >>> Automation-friendly workflow is compatible with liquid handlers >>> Functionally validated with the Illumina GAIIx and HiSeq 2000 sequencing platforms
The NEXTflex qRNA-Seq Kit contains enough material to prepare 8 or 48 RNA samples for Illumina compatible paired end sequencing. The shelf life of all reagents is 6 months when stored properly. All components can safely be stored at -20°C.
For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 5130-13
The patent pending, NEXTflex qRNA-Seq Kit, enables high precision gene expression analysis by RNA-Seq. Developed in conjunction with Cellular Research Inc., this new kit efficiently generates libraries equivalent to conventional RNA-Seq libraries, but with the added feature of molecular indexing. Similar to conventional RNA-Seq, sample RNA is converted to cDNA fragments. But prior to any PCR amplification steps, all DNA fragment ends are ligated to a pair of adapters chosen at random from a total set of 9,216 molecular indices. Individual DNA molecules of identical sequence become distinct through indexing, allowing for differentiation between re-sampling of the same molecule and sampling of a different molecule of identical sequence. Analysis using molecular indexing information provides an absolute, digital measurement of gene expression levels, irrespective of common amplification distortions observed in many RNA-Seq experiments.
[STOCHASTIC LABELING OF INDIVIDUAL DNA MOLECULES] The NEXTflex qRNA-Seq Kit contains two sets of 96 distinct molecular labels on the sequencing adapters. Each label or index consists of an 8 nucleotide barcode tag. In the ligation reaction, these 96 adapters are present in vast molar excess over the concentration of the cDNA fragments, and therefore serve as a non-depleting reservoir of molecular labels. Each end of a cDNA fragment independently and randomly chooses and ligates to a single label from this pool of 96 adapters to result in a total of 96 x 96 = 9,216 possible combinations across both ends. For every clone sequenced, paired-end reads reveals the chosen label on each end along with adjoining cDNA sequence. In addition to encoding DNA fragments at the molecular level, the kit also allows for the application of sample-specific barcodes during the library preparation PCR step. A more detailed description of the use of molecular indexing for RNA-Seq is available in our product application note.
[PROTOCOL] Using the NEXTflex qRNA-Seq Kit, mRNA or rRNA-depleted RNA is fragmented using a cationic buffer. Fragmented RNA undergoes first and second strand synthesis, followed by end-repair, adenylation, ligation and PCR.
[MULTIPLEXING] NEXTflex qRNA-Seq Kits contain barcodes so additional barcodes do not need to be purchased. The 8 rxn kit includes 4 barcodes for multiplexing. Four different versions of the 48 reaction kit are available, each contain 24 unique barcodes. Up to 96 samples can be multiplexed together.
For more information read our recent white paper written (Click the DataSheet link) in collaboration with Cellular Research, which demonstrates the capabilities of the NEXTflex qRNA-Seq Kit for quantitative transcriptome analysis.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
[BENEFITS] >>> Enables high precision measurement of RNA concentration >>> Enhanced Adapter Ligation Technology offers a larger number of unique sequencing reads >>> Input 10 - 100 ng of mRNA or rRNA-depleted total RNA >>> Embedded sample index barcodes for multiplexing of up to 96 samples >>> Automation-friendly workflow is compatible with liquid handlers >>> Functionally validated with Illumina sequencing platforms >>> Automation-friendly workflow is compatible with liquid handlers >>> Functionally validated with the Illumina GAIIx and HiSeq 2000 sequencing platforms
The NEXTflex qRNA-Seq Kit contains enough material to prepare 8 or 48 RNA samples for Illumina compatible paired end sequencing. The shelf life of all reagents is 6 months when stored properly. All components can safely be stored at -20°C.
For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 5130-14
The patent pending, NEXTflex qRNA-Seq Kit, enables high precision gene expression analysis by RNA-Seq. Developed in conjunction with Cellular Research Inc., this new kit efficiently generates libraries equivalent to conventional RNA-Seq libraries, but with the added feature of molecular indexing. Similar to conventional RNA-Seq, sample RNA is converted to cDNA fragments. But prior to any PCR amplification steps, all DNA fragment ends are ligated to a pair of adapters chosen at random from a total set of 9,216 molecular indices. Individual DNA molecules of identical sequence become distinct through indexing, allowing for differentiation between re-sampling of the same molecule and sampling of a different molecule of identical sequence. Analysis using molecular indexing information provides an absolute, digital measurement of gene expression levels, irrespective of common amplification distortions observed in many RNA-Seq experiments.
[STOCHASTIC LABELING OF INDIVIDUAL DNA MOLECULES] The NEXTflex qRNA-Seq Kit contains two sets of 96 distinct molecular labels on the sequencing adapters. Each label or index consists of an 8 nucleotide barcode tag. In the ligation reaction, these 96 adapters are present in vast molar excess over the concentration of the cDNA fragments, and therefore serve as a non-depleting reservoir of molecular labels. Each end of a cDNA fragment independently and randomly chooses and ligates to a single label from this pool of 96 adapters to result in a total of 96 x 96 = 9,216 possible combinations across both ends. For every clone sequenced, paired-end reads reveals the chosen label on each end along with adjoining cDNA sequence. In addition to encoding DNA fragments at the molecular level, the kit also allows for the application of sample-specific barcodes during the library preparation PCR step. A more detailed description of the use of molecular indexing for RNA-Seq is available in our product application note.
[PROTOCOL] Using the NEXTflex qRNA-Seq Kit, mRNA or rRNA-depleted RNA is fragmented using a cationic buffer. Fragmented RNA undergoes first and second strand synthesis, followed by end-repair, adenylation, ligation and PCR.
[MULTIPLEXING] NEXTflex qRNA-Seq Kits contain barcodes so additional barcodes do not need to be purchased. The 8 rxn kit includes 4 barcodes for multiplexing. Four different versions of the 48 reaction kit are available, each contain 24 unique barcodes. Up to 96 samples can be multiplexed together.
For more information read our recent white paper written (Click the DataSheet link) in collaboration with Cellular Research, which demonstrates the capabilities of the NEXTflex qRNA-Seq Kit for quantitative transcriptome analysis.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
[BENEFITS] >>> Enables high precision measurement of RNA concentration >>> Enhanced Adapter Ligation Technology offers a larger number of unique sequencing reads >>> Input 10 - 100 ng of mRNA or rRNA-depleted total RNA >>> Embedded sample index barcodes for multiplexing of up to 96 samples >>> Automation-friendly workflow is compatible with liquid handlers >>> Functionally validated with Illumina sequencing platforms >>> Automation-friendly workflow is compatible with liquid handlers >>> Functionally validated with the Illumina GAIIx and HiSeq 2000 sequencing platforms
The NEXTflex qRNA-Seq Kit contains enough material to prepare 8 or 48 RNA samples for Illumina compatible paired end sequencing. The shelf life of all reagents is 6 months when stored properly. All components can safely be stored at -20°C.
For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 5130-15
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