The NEXTflex Small RNA-Seq Kit v3 uses patented and patent-pending technology to provide a reduced-bias small RNA library preparation solution for Illumina sequencing platforms with gel-free or low-input options. Bioo Scientifics approach to reducing ligation-associated bias involves the use of adapters with randomized bases at the ligation junctions, resulting in greatly decreased bias in comparison to standard protocols. This reduction in bias results in data that more accurately represents abundances of small RNAs in the starting material. In addition, reduction of bias allows more miRNAs to be detected with fewer total reads, increasing efficiency and reducing cost for small RNA sequencing.
PAGE purification, required for traditional small RNA library prep, is tedious, time consuming, limits throughput, and prevents start-to-finish automation. The NEXTflex Small RNA-Seq Kit v3 allows for gel-free small RNA library preparation. This is possible thanks to the dual approach used for adapter-dimer reduction. Unprecedented reduction of adapter-dimer formation allows completely gel-free small RNA library prep when starting with ?200 ng of total RNA. Libraries prepared with the NEXTflex Small RNA-Seq kit v3 have a higher proportion of reads mapping to miRNAs
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
The adapter-dimer reduction technology incorporated into this kit also allows low input library preparation. Library preparation with as little as 5 ng of total RNA is possible as additional PCR cycles can be performed without adapter-dimer products dominating the final library. Fig. 2 illustrated that expression values are reproducible across different sample inputs.
Completely gel-free protocol with normal input amounts Greater discovery/detection rates reduces sequencing cost Randomized adapters reduce ligation bias, resulting in more accurate data Low input (5 ng) gel-based protocol included AIR Ligase offers greater sequencing depth Use more of your RNA sample - 10.5 µL input volume Barcoded primers are included in the kit allowing multiplexing of up to 48 samples Functionally validated with Illumina sequencing platform
Replacement for kit 5132-03
The NEXTflex Small RNA Sequencing Kit v3 contains the reagents, including barcoded primers, to prepare 8 or 48 small RNA samples for Illumina®-compatible next-generation sequencing. The shelf life of all reagents is 6 months when stored properly at -20°C. Ships on dry ice.
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 5132-05
The NEXTflex Small RNA-Seq Kit v3 uses patented and patent-pending technology to provide a reduced-bias small RNA library preparation solution for Illumina sequencing platforms with gel-free or low-input options. Bioo Scientifics approach to reducing ligation-associated bias involves the use of adapters with randomized bases at the ligation junctions, resulting in greatly decreased bias in comparison to standard protocols. This reduction in bias results in data that more accurately represents abundances of small RNAs in the starting material. In addition, reduction of bias allows more miRNAs to be detected with fewer total reads, increasing efficiency and reducing cost for small RNA sequencing.
PAGE purification, required for traditional small RNA library prep, is tedious, time consuming, limits throughput, and prevents start-to-finish automation. The NEXTflex Small RNA-Seq Kit v3 allows for gel-free small RNA library preparation. This is possible thanks to the dual approach used for adapter-dimer reduction. Unprecedented reduction of adapter-dimer formation allows completely gel-free small RNA library prep when starting with ?200 ng of total RNA. Libraries prepared with the NEXTflex Small RNA-Seq kit v3 have a higher proportion of reads mapping to miRNAs
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
The adapter-dimer reduction technology incorporated into this kit also allows low input library preparation. Library preparation with as little as 5 ng of total RNA is possible as additional PCR cycles can be performed without adapter-dimer products dominating the final library. Fig. 2 illustrated that expression values are reproducible across different sample inputs.
Completely gel-free protocol with normal input amounts Greater discovery/detection rates reduces sequencing cost Randomized adapters reduce ligation bias, resulting in more accurate data Low input (5 ng) gel-based protocol included AIR Ligase offers greater sequencing depth Use more of your RNA sample - 10.5 µL input volume Barcoded primers are included in the kit allowing multiplexing of up to 48 samples Functionally validated with Illumina sequencing platform
Replacement for kit 5132-03
The NEXTflex Small RNA Sequencing Kit v3 contains the reagents, including barcoded primers, to prepare 8 or 48 small RNA samples for Illumina®-compatible next-generation sequencing. The shelf life of all reagents is 6 months when stored properly at -20°C. Ships on dry ice.
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 5132-06
The NEXTflex Small RNA-Seq Kit v3 uses patented and patent-pending technology to provide a reduced-bias small RNA library preparation solution for Illumina sequencing platforms with gel-free or low-input options. Bioo Scientifics approach to reducing ligation-associated bias involves the use of adapters with randomized bases at the ligation junctions, resulting in greatly decreased bias in comparison to standard protocols. This reduction in bias results in data that more accurately represents abundances of small RNAs in the starting material. In addition, reduction of bias allows more miRNAs to be detected with fewer total reads, increasing efficiency and reducing cost for small RNA sequencing.
PAGE purification, required for traditional small RNA library prep, is tedious, time consuming, limits throughput, and prevents start-to-finish automation. The NEXTflex Small RNA-Seq Kit v3 allows for gel-free small RNA library preparation. This is possible thanks to the dual approach used for adapter-dimer reduction. Unprecedented reduction of adapter-dimer formation allows completely gel-free small RNA library prep when starting with ?200 ng of total RNA. Libraries prepared with the NEXTflex Small RNA-Seq kit v3 have a higher proportion of reads mapping to miRNAs
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
The adapter-dimer reduction technology incorporated into this kit also allows low input library preparation. Library preparation with as little as 5 ng of total RNA is possible as additional PCR cycles can be performed without adapter-dimer products dominating the final library. Fig. 2 illustrated that expression values are reproducible across different sample inputs.
Completely gel-free protocol with normal input amounts Greater discovery/detection rates reduces sequencing cost Randomized adapters reduce ligation bias, resulting in more accurate data Low input (5 ng) gel-based protocol included AIR Ligase offers greater sequencing depth Use more of your RNA sample - 10.5 µL input volume Barcoded primers are included in the kit allowing multiplexing of up to 48 samples Functionally validated with Illumina sequencing platform
Replacement for kit 5132-03
The NEXTflex Small RNA Sequencing Kit v3 contains the reagents, including barcoded primers, to prepare 8 or 48 small RNA samples for Illumina®-compatible next-generation sequencing. The shelf life of all reagents is 6 months when stored properly at -20°C. Ships on dry ice.
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 5132-06
The NEXTFLEX® small RNA-seq v3 automation kit with UDIs uses the same popular chemistry of the NEXTFLEX small RNA-seq kit v3, which incorporates technologies that reduce small RNA ligation bias, increase ligation efficiency, reduce adapter-dimer formation, and build in a new dimension of data quality, integrity, and accuracy with the integration of Unique Dual Indexes (UDIs) for Illumina® sequencing on the MiSeq®, HiSeq® 2000/2500, and NovaSeq® platforms. The addition of UDI barcodes for small RNA sequencing allows confident multiplexing of up to 192 samples, all while mitigating the risk of index hopping and spread of signal that can occur on a patterned flow cell. The purity of each index sequence is validated by sequencing for high confidence in the resulting sequencing data quality.
The NEXTFLEX® small RNA-seq v3 automation kit with UDIs is compatible with cell-free RNA, such as RNA isolated from plasma. Users who wish to deplete the abundant tRNA fragments and Y RNA fragments found in many types of cell-free RNA should use the NEXTFLEX® tRNA/YRNA blocker (not included in kit). If NEXTFLEX® tRNA/YRNA blockers are of interest,
> Greater discovery/detection rate reduces sequencing costs > Randomized adapter technology reduces ligation bias and increases accuracy > As little as 200 ng input starting material for complete automation > Readily-available barcoded primer set allows multiplexing of up to 192 samples > Unique dual index barcoded primer set mitigates index hopping on the Illumina® NovaSeq®, MiSeq®, and HiSeq® 2000/2500 platforms > Individually sequence-validated indices ensure confidence in purity > Automated kit for the PerkinElmer® Sciclone® G3 NGS/NGSx and the Zephyr® G3 NGS workstation saves time and resources
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
The adapter-dimer reduction technology incorporated into this kit also allows low input library preparation. Library preparation with as little as 5 ng of total RNA is possible as additional PCR cycles can be performed without adapter-dimer products dominating the final library. Fig. 2 illustrated that expression values are reproducible across different sample inputs.
Completely gel-free protocol with normal input amounts Greater discovery/detection rates reduces sequencing cost Randomized adapters reduce ligation bias, resulting in more accurate data Low input (5 ng) gel-based protocol included AIR Ligase offers greater sequencing depth Use more of your RNA sample - 10.5 µL input volume Barcoded primers are included in the kit allowing multiplexing of up to 48 samples Functionally validated with Illumina sequencing platform
The NEXTflex Small RNA Sequencing Kit v3 contains the reagents, including barcoded primers, to prepare 8 or 48 small RNA samples for Illumina®-compatible next-generation sequencing. The shelf life of all reagents is 6 months when stored properly at -20°C. Ships on dry ice.
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
The NEXTFLEX® small RNA-seq v3 automation kit with UDIs uses the same popular chemistry of the NEXTFLEX small RNA-seq kit v3, which incorporates technologies that reduce small RNA ligation bias, increase ligation efficiency, reduce adapter-dimer formation, and build in a new dimension of data quality, integrity, and accuracy with the integration of Unique Dual Indexes (UDIs) for Illumina® sequencing on the MiSeq®, HiSeq® 2000/2500, and NovaSeq® platforms. The addition of UDI barcodes for small RNA sequencing allows confident multiplexing of up to 192 samples, all while mitigating the risk of index hopping and spread of signal that can occur on a patterned flow cell. The purity of each index sequence is validated by sequencing for high confidence in the resulting sequencing data quality.
The NEXTFLEX® small RNA-seq v3 automation kit with UDIs is compatible with cell-free RNA, such as RNA isolated from plasma. Users who wish to deplete the abundant tRNA fragments and Y RNA fragments found in many types of cell-free RNA should use the NEXTFLEX® tRNA/YRNA blocker (not included in kit). If NEXTFLEX® tRNA/YRNA blockers are of interest,
> Greater discovery/detection rate reduces sequencing costs > Randomized adapter technology reduces ligation bias and increases accuracy > As little as 200 ng input starting material for complete automation > Readily-available barcoded primer set allows multiplexing of up to 192 samples > Unique dual index barcoded primer set mitigates index hopping on the Illumina® NovaSeq®, MiSeq®, and HiSeq® 2000/2500 platforms > Individually sequence-validated indices ensure confidence in purity > Automated kit for the PerkinElmer® Sciclone® G3 NGS/NGSx and the Zephyr® G3 NGS workstation saves time and resources
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
The adapter-dimer reduction technology incorporated into this kit also allows low input library preparation. Library preparation with as little as 5 ng of total RNA is possible as additional PCR cycles can be performed without adapter-dimer products dominating the final library. Fig. 2 illustrated that expression values are reproducible across different sample inputs.
Completely gel-free protocol with normal input amounts Greater discovery/detection rates reduces sequencing cost Randomized adapters reduce ligation bias, resulting in more accurate data Low input (5 ng) gel-based protocol included AIR Ligase offers greater sequencing depth Use more of your RNA sample - 10.5 µL input volume Barcoded primers are included in the kit allowing multiplexing of up to 48 samples Functionally validated with Illumina sequencing platform
The NEXTflex Small RNA Sequencing Kit v3 contains the reagents, including barcoded primers, to prepare 8 or 48 small RNA samples for Illumina®-compatible next-generation sequencing. The shelf life of all reagents is 6 months when stored properly at -20°C. Ships on dry ice.
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
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