NEXTflex BRCA1 and BRCA2 Amplicon Panel for Ion Torrent-based Sequencing is a complete solution that targets all coding exons in BRCA1 and BRCA2 loci using 131 sequence validated PCR primer pairs.
This kit requires only 20 ng of DNA from fresh or frozen samples. The convenient predesigned panel allows researchers to focus on data generation and analysis, not on labor-intensive primer design and target selection. While competitor protocols typically require several hours to perform, the NEXTflex BRCA1 & BRCA2 Amplicon Panel makes it possible to produce targeted Ion Torrent-compatible libraries in only 4 hours. Additionally, the NEXTflex BRCA1 & BRCA2 Amplicon Panel protocol can be easily automated, further simplifying workflow.
This kit is not designed for use with DNA isolated from FFPE or cfDNA samples.
Validated BRCA1/2 panel with library prep reagents and 60 barcodes for sequencing on any Ion platform.
[BENEFITS] >>> Complete BRCA1 and BRCA2 coverage >>> 100% uniformity of all targeted coding exons and exon-intron boundaries >>> Simple Ion Torrent targeted library prep protocol - 4 hour protocol validated with low input samples >>> For detection of germline mutations multiplex up to 20 samples at 100x coverage on a single Ion 318TM Chip Kit v2 and for detection of somatic mutations multiplex up to 20 samples at 2000x coverage on Ion Proton >>> Complete solution for targeted sequencing including gene specific primers, library prep reagents, and up to 20 barcodes >>> Low input - only 20 ng DNA required from fresh or frozen tissue or blood
The NEXTflex BRCA1 & BRCA2 Amplicon Panel contains enough material to prepare 8, or 20 samples for Ion Torrent-compatible sequencing. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.
For larger volume requirements, customized and bulk packaging is available. Please email us to tech@nktscientific.com for further information.
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 4040-01
The NEXTflex BRCA1 & BRCA2 Amplicon Panel for FFPE is a complete solution that targets all coding exons in BRCA1 and BRCA2 loci, using 261 sequence validated PCR primer pairs.
This kit requires only 40 ng of DNA. The convenient predesigned panel allows researchers to focus on data generation and analysis, not on labor-intensive primer design and target selection. The NEXTflex BRCA1 & BRCA2 Amplicon Panel for FFPE protocol can be easily automated, further simplifying workflow.
Validated BRCA1/2 panel with library prep reagents and 60 barcodes for sequencing on any Ion platform.
[BENEFITS] >>> Complete BRCA1 and BRCA2 coverage >>> 99% uniformity of all targeted coding exons and exon-intron boundaries, validated on DNA isolated from FFPE samples >>> Simple Ion Torrent targeted library prep protocol - 4 hour protocol >>> For detection of somatic mutations, up to 20 samples can be multiplexed at 2000x coverage on the Ion Proton >>> Complete solution for targeted sequencing, including gene specific primers, library prep reagents, cleanup beads and up to 60 unique barcodes >>> Low input - Only 40 ng DNA isolated from FFPE samples required
The NEXTflex BRCA1 & BRCA2 Amplicon Panel contains enough material to prepare 8, or 20 samples for Ion Torrent-compatible sequencing. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.
For larger volume requirements, customized and bulk packaging is available. Please email us to tech@nktscientific.com for further information.
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 4041-01
NEXTflex BRCA1 and BRCA2 Amplicon Panel for Ion Torrent-based Sequencing is a complete solution that targets all coding exons in BRCA1 and BRCA2 loci using 131 sequence validated PCR primer pairs.
This kit requires only 20 ng of DNA from fresh or frozen samples. The convenient predesigned panel allows researchers to focus on data generation and analysis, not on labor-intensive primer design and target selection. While competitor protocols typically require several hours to perform, the NEXTflex BRCA1 & BRCA2 Amplicon Panel makes it possible to produce targeted Ion Torrent-compatible libraries in only 4 hours. Additionally, the NEXTflex BRCA1 & BRCA2 Amplicon Panel protocol can be easily automated, further simplifying workflow.
This kit is not designed for use with DNA isolated from FFPE or cfDNA samples.
Validated BRCA1/2 panel with library prep reagents and 60 barcodes for sequencing on any Ion platform.
[BENEFITS] >>> Complete BRCA1 and BRCA2 coverage >>> 100% uniformity of all targeted coding exons and exon-intron boundaries >>> Simple Ion Torrent targeted library prep protocol - 4 hour protocol validated with low input samples >>> For detection of germline mutations multiplex up to 20 samples at 100x coverage on a single Ion 318TM Chip Kit v2 and for detection of somatic mutations multiplex up to 20 samples at 2000x coverage on Ion Proton >>> Complete solution for targeted sequencing including gene specific primers, library prep reagents, and up to 20 barcodes >>> Low input - only 20 ng DNA required from fresh or frozen tissue or blood
The NEXTflex BRCA1 & BRCA2 Amplicon Panel contains enough material to prepare 8, or 20 samples for Ion Torrent-compatible sequencing. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.
For larger volume requirements, customized and bulk packaging is available. Please email us to tech@nktscientific.com for further information.
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 4040-02
NEXTflex BRCA1 and BRCA2 Amplicon Panel for Ion Torrent-based Sequencing is a complete solution that targets all coding exons in BRCA1 and BRCA2 loci using 131 sequence validated PCR primer pairs.
This kit requires only 20 ng of DNA from fresh or frozen samples. The convenient predesigned panel allows researchers to focus on data generation and analysis, not on labor-intensive primer design and target selection. While competitor protocols typically require several hours to perform, the NEXTflex BRCA1 & BRCA2 Amplicon Panel makes it possible to produce targeted Ion Torrent-compatible libraries in only 4 hours. Additionally, the NEXTflex BRCA1 & BRCA2 Amplicon Panel protocol can be easily automated, further simplifying workflow.
This kit is not designed for use with DNA isolated from FFPE or cfDNA samples.
Validated BRCA1/2 panel with library prep reagents and 60 barcodes for sequencing on any Ion platform.
[BENEFITS] >>> Complete BRCA1 and BRCA2 coverage >>> 100% uniformity of all targeted coding exons and exon-intron boundaries >>> Simple Ion Torrent targeted library prep protocol - 4 hour protocol validated with low input samples >>> For detection of germline mutations multiplex up to 20 samples at 100x coverage on a single Ion 318TM Chip Kit v2 and for detection of somatic mutations multiplex up to 20 samples at 2000x coverage on Ion Proton >>> Complete solution for targeted sequencing including gene specific primers, library prep reagents, and up to 20 barcodes >>> Low input - only 20 ng DNA required from fresh or frozen tissue or blood
The NEXTflex BRCA1 & BRCA2 Amplicon Panel contains enough material to prepare 8, or 20 samples for Ion Torrent-compatible sequencing. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.
For larger volume requirements, customized and bulk packaging is available. Please email us to tech@nktscientific.com for further information.
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 4040-03
NEXTflex BRCA1 and BRCA2 Amplicon Panel for Ion Torrent-based Sequencing is a complete solution that targets all coding exons in BRCA1 and BRCA2 loci using 131 sequence validated PCR primer pairs.
This kit requires only 20 ng of DNA from fresh or frozen samples. The convenient predesigned panel allows researchers to focus on data generation and analysis, not on labor-intensive primer design and target selection. While competitor protocols typically require several hours to perform, the NEXTflex BRCA1 & BRCA2 Amplicon Panel makes it possible to produce targeted Ion Torrent-compatible libraries in only 4 hours. Additionally, the NEXTflex BRCA1 & BRCA2 Amplicon Panel protocol can be easily automated, further simplifying workflow.
This kit is not designed for use with DNA isolated from FFPE or cfDNA samples.
Validated BRCA1/2 panel with library prep reagents and 60 barcodes for sequencing on any Ion platform.
[BENEFITS] >>> Complete BRCA1 and BRCA2 coverage >>> 100% uniformity of all targeted coding exons and exon-intron boundaries >>> Simple Ion Torrent targeted library prep protocol - 4 hour protocol validated with low input samples >>> For detection of germline mutations multiplex up to 20 samples at 100x coverage on a single Ion 318TM Chip Kit v2 and for detection of somatic mutations multiplex up to 20 samples at 2000x coverage on Ion Proton >>> Complete solution for targeted sequencing including gene specific primers, library prep reagents, and up to 20 barcodes >>> Low input - only 20 ng DNA required from fresh or frozen tissue or blood
The NEXTflex BRCA1 & BRCA2 Amplicon Panel contains enough material to prepare 8, or 20 samples for Ion Torrent-compatible sequencing. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.
For larger volume requirements, customized and bulk packaging is available. Please email us to tech@nktscientific.com for further information.
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 4040-04
The NEXTflex BRCA1 & BRCA2 Amplicon Panel for FFPE is a complete solution that targets all coding exons in BRCA1 and BRCA2 loci, using 261 sequence validated PCR primer pairs.
This kit requires only 40 ng of DNA. The convenient predesigned panel allows researchers to focus on data generation and analysis, not on labor-intensive primer design and target selection. The NEXTflex BRCA1 & BRCA2 Amplicon Panel for FFPE protocol can be easily automated, further simplifying workflow.
Validated BRCA1/2 panel with library prep reagents and 60 barcodes for sequencing on any Ion platform.
[BENEFITS] >>> Complete BRCA1 and BRCA2 coverage >>> 99% uniformity of all targeted coding exons and exon-intron boundaries, validated on DNA isolated from FFPE samples >>> Simple Ion Torrent targeted library prep protocol - 4 hour protocol >>> For detection of somatic mutations, up to 20 samples can be multiplexed at 2000x coverage on the Ion Proton >>> Complete solution for targeted sequencing, including gene specific primers, library prep reagents, cleanup beads and up to 60 unique barcodes >>> Low input - Only 40 ng DNA isolated from FFPE samples required
The NEXTflex BRCA1 & BRCA2 Amplicon Panel contains enough material to prepare 8, or 20 samples for Ion Torrent-compatible sequencing. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.
For larger volume requirements, customized and bulk packaging is available. Please email us to tech@nktscientific.com for further information.
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 4041-02
The NEXTflex BRCA1 & BRCA2 Amplicon Panel for FFPE is a complete solution that targets all coding exons in BRCA1 and BRCA2 loci, using 261 sequence validated PCR primer pairs.
This kit requires only 40 ng of DNA. The convenient predesigned panel allows researchers to focus on data generation and analysis, not on labor-intensive primer design and target selection. The NEXTflex BRCA1 & BRCA2 Amplicon Panel for FFPE protocol can be easily automated, further simplifying workflow.
Validated BRCA1/2 panel with library prep reagents and 60 barcodes for sequencing on any Ion platform.
[BENEFITS] >>> Complete BRCA1 and BRCA2 coverage >>> 99% uniformity of all targeted coding exons and exon-intron boundaries, validated on DNA isolated from FFPE samples >>> Simple Ion Torrent targeted library prep protocol - 4 hour protocol >>> For detection of somatic mutations, up to 20 samples can be multiplexed at 2000x coverage on the Ion Proton >>> Complete solution for targeted sequencing, including gene specific primers, library prep reagents, cleanup beads and up to 60 unique barcodes >>> Low input - Only 40 ng DNA isolated from FFPE samples required
The NEXTflex BRCA1 & BRCA2 Amplicon Panel contains enough material to prepare 8, or 20 samples for Ion Torrent-compatible sequencing. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.
For larger volume requirements, customized and bulk packaging is available. Please email us to tech@nktscientific.com for further information.
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 4041-03
The NEXTflex BRCA1 & BRCA2 Amplicon Panel for FFPE is a complete solution that targets all coding exons in BRCA1 and BRCA2 loci, using 261 sequence validated PCR primer pairs.
This kit requires only 40 ng of DNA. The convenient predesigned panel allows researchers to focus on data generation and analysis, not on labor-intensive primer design and target selection. The NEXTflex BRCA1 & BRCA2 Amplicon Panel for FFPE protocol can be easily automated, further simplifying workflow.
Validated BRCA1/2 panel with library prep reagents and 60 barcodes for sequencing on any Ion platform.
[BENEFITS] >>> Complete BRCA1 and BRCA2 coverage >>> 99% uniformity of all targeted coding exons and exon-intron boundaries, validated on DNA isolated from FFPE samples >>> Simple Ion Torrent targeted library prep protocol - 4 hour protocol >>> For detection of somatic mutations, up to 20 samples can be multiplexed at 2000x coverage on the Ion Proton >>> Complete solution for targeted sequencing, including gene specific primers, library prep reagents, cleanup beads and up to 60 unique barcodes >>> Low input - Only 40 ng DNA isolated from FFPE samples required
The NEXTflex BRCA1 & BRCA2 Amplicon Panel contains enough material to prepare 8, or 20 samples for Ion Torrent-compatible sequencing. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.
For larger volume requirements, customized and bulk packaging is available. Please email us to tech@nktscientific.com for further information.
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 4041-04
NGS Applications:
BRCA1 and BRCA2 Panels
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