Cholesterol 24-hydroxylase (CYP46A1) is a cytochrome P450 enzyme and endoplasmic reticulum protein expressed in brain tissues, It is important in brain cholesterol homeostasis being responsible for cholesterol elimination by its hydroxylation into 24S-hydroxycholesterol. Cholesterol cannot cross the blood-brain barrier, but 24S-hydroxycholesterol can, allowing it to travel via the circulatory system to the liver for catabolism. Increasing evidence suggests that CYP46A1 has a role in the pathogenesis and progression of neurodegenerative disorders with there being significant CYP46A1 expression in astrocytes and around amyloid plaques in Alzheimers disease, and increases in the level of CYP46A1 in the brain having a possible neuroprotective role in Huntingtons disease.
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal
Format:
50 µg in 50 µl PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Temp:
Store at -20°C. Avoid freeze / thaw cycles.
Host Animal:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Expected Species:
Pig, Bovine, Horse
If you would like us to check if this antibody is likely to bind to this protein from a different species please contact us. We are happy to check for you.
Immunogen:
Partial length recombinant human CYP46A1 from the N-terminal region
If you would like further information regarding the immunogen used in the productiion of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
Involved in the turnover of cholesterol. It converts cholesterol into 24S-hydroxycholesterol and, to a lesser extent, 25-hydroxycholesterol. Has also activity with xenobiotic compounds, such as clotrimazole.
Cytochrome P450 46A1 Antibody detects endogenous levels of total Cytochrome P450 46A1
Immunogen:
A synthesized peptide derived from human Cytochrome P450 46A1
Cross Reactivity:
Human,Mouse
Conjugate:
Unconjugated
purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink(TM) Coupling Resin (Thermo Fisher Scientific).
Concentration:
1mg/ml
Fragment:
Fab fragment
Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt
Cholesterol 24-hydroxylase (CYP46A1) is a cytochrome P450 enzyme and endoplasmic reticulum protein expressed in brain tissues, It is important in brain cholesterol homeostasis being responsible for cholesterol elimination by its hydroxylation into 24S-hydroxycholesterol. Cholesterol cannot cross the blood-brain barrier, but 24S-hydroxycholesterol can, allowing it to travel via the circulatory system to the liver for catabolism. Increasing evidence suggests that CYP46A1 has a role in the pathogenesis and progression of neurodegenerative disorders with there being significant CYP46A1 expression in astrocytes and around amyloid plaques in Alzheimers disease, and increases in the level of CYP46A1 in the brain having a possible neuroprotective role in Huntingtons disease.
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal
Format:
100 µg in 100 µl PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Temp:
Store at -20°C. Avoid freeze / thaw cycles.
Host Animal:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Expected Species:
Pig, Bovine, Horse
If you would like us to check if this antibody is likely to bind to this protein from a different species please contact us. We are happy to check for you.
Immunogen:
Partial length recombinant human CYP46A1 from the N-terminal region
If you would like further information regarding the immunogen used in the productiion of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
Involved in the turnover of cholesterol. It converts cholesterol into 24S-hydroxycholesterol and, to a lesser extent, 25-hydroxycholesterol. Has also activity with xenobiotic compounds, such as clotrimazole.
Cytochrome P450 46A1 Antibody detects endogenous levels of total Cytochrome P450 46A1
Immunogen:
A synthesized peptide derived from human Cytochrome P450 46A1
Cross Reactivity:
Human,Mouse
Conjugate:
Unconjugated
purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink(TM) Coupling Resin (Thermo Fisher Scientific).
Concentration:
1mg/ml
Fragment:
Fab fragment
Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt
Cytochrome P450 46A1 belongs to the cytochrome P450 family which are a group of monooxygenases. Cytochrome P450 46A1 is involved in the turnover of cholesterol converting it into 24S-hydroxycholesterol and, to a lesser extent, 25-hydroxycholesterol. This protein is expressed in the brain and mRNA has been found to be broadly distributed with higher levels in the gray matter zones and lower levels in white matter areas. It is not detected in fetal brain but its expression increases linearly with age. It localises to the endoplasmic reticulum membrane and the microsome membrane. The unprocessed precursor is 500 amino acids long and has a predicted molecular weight of 56.8KDa.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS pH 7.2
Storage Temp:
After reconstitution keep aliquots at -20 °C for higher stability or at 4 °C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Partial recombinant protein of human cytochrome P450 46A1 (amino acids 201 to 300) with a GST tag.
This antibody is recommended for WB and direct and sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Cholesterol 24-hydroxylase; CH24H; CYP46A1; CYP46
Accession Number:
Q9Y6A2_HUMAN
Reconstitution:
Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Shelf Life:
12 months after purchase
Specificity:
The specificity of this antibody has been confirmed by WB and ELISA against the antigen. | Other species have not yet been tested.
Purity:
Protein G purified immunoglobulin
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