HBM provides a ready to use kit for the qualitative FACS analysis of exosomes. The Exo-FACS assay for exosome characterisation consists of 4um beads used for the direct overall capture of pre-purified, validated and lyophilized exosomes from cell culture supernatants or human biological fluids. The characterization of exosomal proteins (membrane expressed or internal) is subsequently performed using appropriate detection antibodies against exosome associated antigens.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
FACS analysis
Storage Temp:
+ 4 C
Applications:
FACS Exosome isolation and exosome marker characterization via FACS. Exosome comprehensive profiling.
No initial exosome purification required User friendly and reliable and efficient Suitable for multiple marker analyses Available in custom format
HBM provides a ready to use kit for the qualitative FACS analysis of exosomes. The Exo-FACS assay for exosome characterisation consists of 4um beads used for the direct overall capture of pre-purified, validated and lyophilized exosomes from cell culture supernatants or human biological fluids. The characterization of exosomal proteins (membrane expressed or internal) is subsequently performed using appropriate detection antibodies against exosome associated antigens.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
FACS analysis
Storage Temp:
+ 4 C
Applications:
FACS Exosome isolation and exosome marker characterization via FACS. Exosome comprehensive profiling.
No initial exosome purification required User friendly and reliable and efficient Suitable for multiple marker analyses Available in custom format
The ExoTEST Ready to Use Kit is the ideal platform for capturing and quantifying exosomes from biological samples. It features ELISA plate(s) pre-coated with primary antibodies to exosome-specific markers, plus the secondary antibody, substrate reagents and the exosome standards. Plus we can also help you customise your own kit by choosing from the individual reagents available in our catalog.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
FACS analysis
Storage Temp:
+ 4 C
Applications:
FACS Exosome isolation and exosome marker characterization via FACS. Exosome comprehensive profiling.
ExoTEST has been developed to fill the need for a reliable method to quantify exosomes and examine their biomarkers. >>>> HansaBioMed also provide the ability to design and create your own kit by choosing from among the wide variety of reagents available in our catalog. 1-Select the exosome standard you need. 2-Select the primary antibody for exosome detection.
HBM provides a ready to use kit for the qualitative FACS analysis of exosomes. The Exo-FACS assay for exosome characterisation consists of 4um beads used for the direct overall capture of pre-purified, validated and lyophilized exosomes from cell culture supernatants or human biological fluids. The characterization of exosomal proteins (membrane expressed or internal) is subsequently performed using appropriate detection antibodies against exosome associated antigens.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
FACS analysis
Storage Temp:
+ 4 C
Applications:
FACS Exosome isolation and exosome marker characterization via FACS. Exosome comprehensive profiling.
ExoTEST has been developed to fill the need for a reliable method to quantify exosomes and examine their biomarkers. >>>> HansaBioMed also provide the ability to design and create your own kit by choosing from among the wide variety of reagents available in our catalog. 1-Select the exosome standard you need. 2-Select the primary antibody for exosome detection.
Up to 100 ul of biological sample can be loaded per well.
Concentrated cell culture supernatant samples are recommended prior capture according to our suggested protocol.
Assay type:
Sandwich enzyme-linked immunoassay.
Sensitivity:
The detection limit of an assay is lower than 0.35 ug of overall exosome which is an equivalent of less than 50 pg of targeted exosomes protein.
Product overview: ExoTEST Ready to Use Kit is the ideal platform for capturing and the quantifying exosomes from cell culture supernatant. ExoTEST Ready to Use Kit features pre-coated ELISA plate with primary antibodies detection directed against exosome-specific markers, namely ?-CD9. Secondary antibodies and substrate reagents are included in the kit. Exosome standards for assay calibration are also included in the kit. Exosome standards are purified by ultracentrifugation and microfiltration, quantified for overall protein content and exosome number and then lyophilized for long-term storage.
ExoTEST ready to use kits are reliable, easy to use, works on a variety of commercially available detection technologies.
Primary antibody: ?-CD9 mouse antibody.
Exosome standard: Lyophilized exosomes from COLO-1 Cell culture supernatant (see details).
Assay protocol: Kit contains all the reagents necessary for exosome capture and protein targeted detection.
Assay calibration: Kit contains exosome standards prepared from purified from proprietary human cell line for assay calibration. All exosome standards are lyophilized and quantified for overall protein content and exosomes number.
HansaBioMed also provides a range of exosome standards purified from different types of human cells that can be purchased separately or included in custom kits in order to meet the need for detection of specific exosome markers.
Background Info:
Sample type: Cell culture supernatant.
Starting material: Up to 100 ul of biological sample can be loaded per well. Concentrated cell culture supernatant samples are recommended prior capture according to our suggested protocol.
Assay type: Sandwich enzyme-linked immunoassay.
Sensitivity: The detection limit of an assay is lower than 0.35 ug of overall exosome which is an equivalent of less than 50 pg of targeted exosomes protein.
Product overview: ExoTEST Ready to Use Kit is the ideal platform for capturing and the quantifying exosomes from cell culture supernatant. ExoTEST Ready to Use Kit features pre-coated ELISA plate with primary antibodies detection directed against exosome-specific markers, namely ?-CD9. Secondary antibodies and substrate reagents are included in the kit. Exosome standards for assay calibration are also included in the kit. Exosome standards are purified by ultracentrifugation and microfiltration, quantified for overall protein content and exosome number and then lyophilized for long-term storage.
ExoTEST ready to use kits are reliable, easy to use, works on a variety of commercially available detection technologies.
Primary antibody: ?-CD9 mouse antibody.
Exosome standard: Lyophilized exosomes from COLO-1 Cell culture supernatant (see details).
Assay protocol: Kit contains all the reagents necessary for exosome capture and protein targeted detection.
Assay calibration: Kit contains exosome standards prepared from purified from proprietary human cell line for assay calibration. All exosome standards are lyophilized and quantified for overall protein content and exosomes number.
HansaBioMed also provides a range of exosome standards purified from different types of human cells that can be purchased separately or included in custom kits in order to meet the need for detection of specific exosome markers.
Product Type:
immunoplate
Storage Temp:
+ 4 C
Applications:
Exosome capture and quantification
Additional Info:
Kit contains all the reagents necessary for exosome capture and protein targeted detection. Assay calibration: Kit contains exosome standards prepared from purified from proprietary human cell line for assay calibration. All exosome standards are lyophilized and quantified for overall protein content and exosomes number. HansaBioMed also provides a range of exosome standards purified from different types of human cells that can be purchased separately or included in custom kits in order to meet the need for detection of specific exosome markers.
Application Details:
Exosome capture and quantification using the ExoTEST ensures dose-dependent exosome detection in a variety of samples. The quantification can be both relative and absolute as the assay calibration enables the calculation of the approximate exosome quantity for each sample.
Comprehensive exosome profiling by implementing different combinations of capture and detection antibodies. ExoTEST can be customized for assessing multiple antigens in a total exosome population within a sample. Informative protein profiles for the samples of interest can be combined with subsequent nucleic acid (mRNA or miRNA) extraction and downstream analysis from captured exosomes.
Specific diagnostic applications using the ExoTEST allows the quantitative assessment of total circulating exosomes and specific exosomal sub-populations, defined by differentially expressed antigens under normal and pathological conditions.
Starting material: Up to 100 ul of biological sample can be loaded per well. Unfractionated plasma sample can be directly used for capture. Concentrated urine samples are recommended prior capture according to our suggested protocol.
Assay type: Sandwich enzyme-linked immunoassay.
Sensitivity: The detection limit of an assay is lower than 0.35 ug of overall exosome which is an equivalent of less than 50 pg of targeted exosomes protein.
Product overview: ExoTEST Ready to Use Kit is the ideal platform for capturing and the quantifying exosomes from biological samples such as plasma, serum, urine and cell culture supernatant. ExoTEST Ready to Use Kit features pre-coated ELISA plate with primary antibodies detection directed against exosome-specific markers, namely ?-CD9. Secondary antibodies and substrate reagents are included in the kit. Exosome standards for assay calibration are also included in the kit. Exosome standards are purified by ultracentrifugation and microfiltration, quantified for overall protein content and exosome number and then lyophilized for long-term storage. ExoTEST ready to use kits are reliable, easy to use, works on a variety of commercially available detection technologies.
Primary antibody: ?-CD9 murine antibody.
Exosome standard: Lyophilized exosomes from COLO-1 Cell culture supernatant (see details).
Assay protocol: Kit contains all the reagents necessary for exosome capture and protein targeted detection.
Assay calibration: kit contains exosome standards for assay calibration. All exosome standards are lyophilized and quantified for overall protein content and exosomes number.
This standards enable both absolute and relative quantification exosomes in analyzed samples.
Product Type:
immunoplate
Storage Temp:
+ 4 C
Applications:
Exosome capture and quantification
Additional Info:
Kit contains all the reagents necessary for exosome capture and protein targeted detection. Assay calibration: Kit contains exosome standards for assay calibration. Depended on the sample type used, kit may include human plasma, serum or urine exosomes. All exosome standards are lyophilized and quantified for overall protein content and exosomes number. This standards enable both absolute and relative quantification exosomes in analyzed samples.
Application Details:
Exosome capture and quantification using the ExoTEST ensures dose-dependent exosome detection in a variety of samples. The quantification can be both relative and absolute as the assay calibration enables the calculation of the approximate exosome quantity for each sample.
Comprehensive exosome profiling by implementing different combinations of capture and detection antibodies. ExoTEST can be customized for assessing multiple antigens in a total exosome population within a sample. Informative protein profiles for the samples of interest can be combined with subsequent nucleic acid (mRNA or miRNA) extraction and downstream analysis from captured exosomes.
Specific diagnostic applications using the ExoTEST allows the quantitative assessment of total circulating exosomes and specific exosomal sub-populations, defined by differentially expressed antigens under normal and pathological conditions.
ExoTEST Ready to Use Kit is the ideal platform for capturing and the quantifying exosomes from biological samples such as plasma and urine. ExoTEST Ready to Use Kit features pre-coated ELISA plate with primary antibodies detection directed against exosome-specific markers, namely ?-CD9. Secondary antibodies and substrate reagents are included in the kit. Exosome standards for assay calibration are also included in the kit. Exosome standards are purified by ultracentrifugation and microfiltration, quantified for overall protein content and exosome number and then lyophilized for long-term storage. ExoTEST ready to use kits are reliable, easy to use, works on a variety of commercially available detection technologies.
Background Info:
Sample type: Plasma
Starting material: Up to 100 ul of biological sample can be loaded per well. Unfractionated plasma sample can be directly used for capture. Concentrated urine samples are recommended prior capture according to our suggested protocol.
Assay type: Sandwich enzyme-linked immunoassay.
Sensitivity: The detection limit of an assay is lower than 0.35 ug of overall exosome which is an equivalent of less than 50 pg of targeted exosomes protein.
Product overview: ExoTEST Ready to Use Kit is the ideal platform for capturing and the quantifying exosomes from biological samples such as plasma and urine.
ExoTEST Ready to Use Kit features pre-coated ELISA plate with primary antibodies detection directed against exosome-specific markers, namely ?-CD9. Secondary antibodies and substrate reagents are included in the kit.
Exosome standards for assay calibration are also included in the kit. Exosome standards are purified by ultracentrifugation and microfiltration, quantified for overall protein content and exosome number and then lyophilized for long-term storage.
ExoTEST ready to use kits are reliable, easy to use, works on a variety of commercially available detection technologies.
Primary antibody: ?-CD9 mouse antibody
Exosome standard: Lyophilized exosomes from plasma of healthy donors (see details).
Assay protocol: Kit contains all the reagents necessary for exosome capture and protein targeted detection.
Assay calibration: Kit contains exosome standards for assay calibration. Depended on the sample type used, kit may include human plasma, serum or urine exosomes. All exosome standards are lyophilized and quantified for overall protein content and exosomes number.
This standards enable both absolute and relative quantification exosomes in analyzed samples.
Product Type:
immunoplate
Storage Temp:
+ 4 C
Applications:
Exosome capture and quantification
Additional Info:
Kit contains all the reagents necessary for exosome capture and protein targeted detection. Assay calibration: Kit contains exosome standards for assay calibration. Depended on the sample type used, kit may include human plasma, serum or urine exosomes. All exosome standards are lyophilized and quantified for overall protein content and exosomes number. This standards enable both absolute and relative quantification exosomes in analyzed samples.
Application Details:
Exosome capture and quantification using the ExoTEST ensures dose-dependent exosome detection in a variety of samples. The quantification can be both relative and absolute as the assay calibration enables the calculation of the approximate exosome quantity for each sample.
Comprehensive exosome profiling by implementing different combinations of capture and detection antibodies. ExoTEST can be customized for assessing multiple antigens in a total exosome population within a sample. Informative protein profiles for the samples of interest can be combined with subsequent nucleic acid (mRNA or miRNA) extraction and downstream analysis from captured exosomes.
Specific diagnostic applications using the ExoTEST allows the quantitative assessment of total circulating exosomes and specific exosomal sub-populations, defined by differentially expressed antigens under normal and pathological conditions.
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