Neurofilaments contain three intermediate filament proteins: light (68 kDa), medium (160 kDa) and heavy (200 kDa). Neurofilament heavy (NF200 or NF-H) is phosphorylated and it is thought that this results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. This antibody recognises both phosphorylated and dephosphorylated forms of NF200/NF-H.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from 1.2% sodium acetate, 2mg BSA, 0.01mg NaN3
Storage Temp:
At least 12 months after purchase at 2 - 4 °C (lyophilized formulations). After reconstitution, aliquot and store at -20 °C for a higher stability. Avoid freeze-thaw cycles.
Host Animal:
Mouse
Species Reactivity:
Human, Mouse, Rat
Immunogen:
C-terminal segment of enzymatically dephosphorylated pig Neurofilament Heavy (NF200).
Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.50 µg/mL is recommended for WB. Human NF200 has a predicted length of 1026 residues and MW of 112 kDa. A concentration of 1.0-2.0 µg/mL is recommended to detect NF200 in formalin fixed and paraffin embedded tissues as well as formalin/acetone fixed tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
NF-200; NF200; NF-H; NEFH; N52; Neurofilament heavy polypeptide; Neurofilament triplet H protein; 200 kDa neurofilament protein; KIAA0845; NFH;
Accession Number:
P12036 NFH_HUMAN; P12037 NFH_PIG
Reconstitution:
Reconstitute in 1 mL of PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Shelf Life:
12 months after purchase.
Specificity:
The specificity of this antibody has been confirmed by WB and IHC against the antigen. | Human; mouse; rat;
References:
Santos J et al., (2017) "Proteomic Analysis of Human Adipose Derived Stem Cells during Small Molecule Chemical Stimulated Pre-neuronal Differentiation." Int J Stem Cells. 2017; 10(2):193-217. Application: WB. Species: Human
Neurofilaments are composed of three intermediate filament proteins: light (~68 kDa), medium (~160 kDa) and heavy (~200 kDa), which are involved in the maintenance of the neuronal caliber. Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized with 5% trehalose
Storage Temp:
After reconstitution of lyophilised antibody, aliquot and store at -20 °C for a higher stability. Avoid freeze-thaw cycles.
Host Animal:
Mouse
Species Reactivity:
Hu, Rat, Ms, Fel, Bov, Por, Chk
Immunogen:
Raised against a recombinant fusion protein containing the extreme C-terminus of rat NF-M expressed in and purified from E. coli. The epitope is localized to within the last 56 amino acids at the extreme C-terminus of rat NF-M, the so-called KE segment which is highly conserved between NF-M molecules from different species.
Western Blotting (WB), Immunocytochemistry (IC), Immunohistochemistry (IH) and Flow Cytometry. A dilution of 1:2,000 - 1:10,000 is recommended for WB. A dilution of 1:1,000 - 1:5,000 is recommended for IC and IH. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Neurofilament medium polypeptide; NF-M; 160 kDa neurofilament protein; Neurofilament 3; Neurofilament triplet M protein; Nefm; Nef3; Nfm;
Accession Number:
P12839 NFM_RAT;
Reconstitution:
Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material.
Shelf Life:
12 months after purchase
Specificity:
Specifically recognizes the medium neurofilament subunit NF-L in WB. | Hu, Rat, Ms, Fel, Bov, Por, Chk
References:
1. Felitsyn N. et al (2008) The heme precursor delta-aminolevulinate blocks peripheral myelin formation. J Neurochem. 2008 Sep;106(5):2068-79.
Neurofilaments contain three intermediate filament proteins: light (68 kDa), medium (160 kDa) and heavy (200 kDa). Neurofilament heavy (NF200 or NF-H) is phosphorylated and it is thought that this results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. This antibody binds primarily to the phosphorylated axonal forms of NF-H.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized with 5% trehalose
Storage Temp:
After reconstitution of lyophilised antibody, aliquot and store at -20 °C for a higher stability. Avoid freeze-thaw cycles.
Host Animal:
Chicken
Species Reactivity:
Hu, Rat, Ms, Fel, Chk, Other Mammalian (Predicted)
Western Blotting (WB), Immunocytochemistry (IC) and ELISA. Suggested dilution for WB is 1:100,000-1,000,000. Suggested dilution for IC is 1:50,000-100,000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
NF-200; NF200; NF-H; NEFH; N52; Neurofilament heavy polypeptide; Neurofilament triplet H protein; 200 kDa neurofilament protein; KIAA0845; NFH;
Accession Number:
P12036 NFH_HUMAN;
Reconstitution:
Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material.
Shelf Life:
12 months after purchase
Specificity:
This antibody reacts with phosphorylated NF-H and is seen as a band of approx 200 kDa in WB. Refer to publication by Shaw et al (2005) for the use of this antibody in an ELISA to detect NF-H. | Hu, Rat, Ms, Fel, Chk. Predicted to react with other mammalian tissues due to sequence homology.
References:
1. Jarjour A.A. et al (2007) Maintenance of axo-oligodendroglial paranodal junctions requires DCC and netrin-1. J Neurosci. 2008 Oct 22;28(43):11003-14.2. Pearse D.D. et al (2007) Transplantation of Schwann cells and/or olfactory ensheathing glia into the contused spinal cord: Survival, migration, axon association, and functional recovery. Glia. 2007 Jul;55(9):976-1000.3. Shaw G. et al (2005) Hyperphosphorylated neurofilament NF-H is a serum biomarker of axonal injury. Biochem Biophys Res Commun. 2005 Nov 4;336(4):1268-77.
Neurofilaments are composed of three intermediate filament proteins: light (~68 kDa), medium (~160 kDa) and heavy (~200 kDa), which are involved in the maintenance of the neuronal caliber. Neurofilament light (NF68 or NF-L) is the most abundant of the three proteins.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized with 5% trehalose
Storage Temp:
After reconstitution of lyophilised antibody, aliquot and store at -20 °C for a higher stability. Avoid freeze-thaw cycles.
Host Animal:
Chicken
Species Reactivity:
Hu, Rat, Ms, Fel, Chk, Other Mammalian (Predicted)
Immunogen:
Purified porcine NF-L from spinal cord and recombinant NF-L.
Western Blotting (WB), Immunocytochemistry (IC), Immunohistochemistry (IHC). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:1,000 - 1:5,000 is recommended for IC, IHC. IHC: 4% Frozen tissues, permeabilized; IH(P): capable, HEIR treatment typically necessary; Chicken IgY can bind non-specifically to tissues, special chicken block solutions available elsewhere are recommended if used on tissues. Dilutions 1:5000 or greater; optimization on time, method required for best results Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material.
Shelf Life:
12 months after purchase
Specificity:
The specificity of this antibody has been confirmed by IC. | Hu, Rat, Ms, Fel, Chk. Predicted to react with other mammalian tissues due to sequence homology.
References:
1. Rangaraju S. et al (2009) Molecular architecture of myelinated peripheral nerves is supported by calorie restriction with aging. Aging Cell. 2009 Apr;8(2):178-91.
Neurofilaments are composed of three intermediate filament proteins: light (~68 kDa), medium (~160 kDa) and heavy (~200 kDa), which are involved in the maintenance of the neuronal caliber. Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized with 5% trehalose
Storage Temp:
After reconstitution of lyophilised antibody, aliquot and store at -20 °C for a higher stability. Avoid freeze-thaw cycles.
Host Animal:
Chicken
Species Reactivity:
Hu, Rat, Ms, Fel, Chk, Other Mammalian (Predicted)
Immunogen:
Recombinant fusion protein containing the extreme C-terminal segment of rat NF-M.
Western Blotting (WB), Immunocytochemistry (IC), Immunohistochemistry (IHC). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:1,000 - 1:3,000 is recommended for IC, IHC. IHC: 4% Frozen tissues, permeabilized; IH(P): capable, HEIR treatment typically necessary; Chicken IgY can bind non-specifically to tissues, special chicken block solutions available elsewhere are recommended if used on tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Neurofilament medium polypeptide; NF-M; 160 kDa neurofilament protein; Neurofilament 3; Neurofilament triplet M protein; Nefm; Nef3; Nfm;
Accession Number:
P12839 NFM_RAT;
Reconstitution:
Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material.
Shelf Life:
12 months after purchase
Specificity:
Specifically recognizes the medium neurofilament subunit NF-L in WB. Band appears at ~145 kDa in WB from rodent and ~160 kDa for human and bovine WB. | Hu, Rat, Ms, Fel, Chk. Predicted to react with other mammalian tissues due to sequence homology.
References:
1. Jarjour A.A. et al (2007) Maintenance of axo-oligodendroglial paranodal junctions requires DCC and netrin-1. J Neurosci. 2008 Oct 22;28(43):11003-14.2. Rangaraju S. et al (2009) Molecular architecture of myelinated peripheral nerves is supported by calorie restriction with aging. Aging Cell. 2009 Apr;8(2):178-91.3. Pearse D.D. et al (2007) Transplantation of Schwann cells and/or olfactory ensheathing glia into the contused spinal cord: Survival, migration, axon association, and functional recovery. Glia. 2007 Jul;55(9):976-1000.4. Shaw G. et al (2004) Characterization of the bovine neurofilament NF-M protein and cDNA sequence, and identification of in vitro and in vivo calpain cleavage sites. Biochem Biophys Res Commun. 2004 Dec 10;325(2):619-25.
Neurofilaments are composed of three intermediate filament proteins: light (~68 kDa), medium (~160 kDa) and heavy (~200 kDa), which are involved in the maintenance of the neuronal caliber. Neurofilament light (NF68 or NF-L) is the most abundant of the three proteins.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized with 5% trehalose
Storage Temp:
After reconstitution of lyophilised antibody, aliquot and store at -20 °C for a higher stability. Avoid freeze-thaw cycles.
Host Animal:
Mouse
Species Reactivity:
Hu, Rat, Ms, Fel, Bov, Por, Chk
Immunogen:
A preparation of enzymatically dephosphorylated pig neurofilaments including NF-L, NF-M and NF-H. Screening was by ELISA on the immungen followed by immunofluorescence microscopy. The epitope for this antibody is not in the N-terminus but its exact location is not known. It is thought to be somewhere within the central alpha helical "rod" region of the molecule.
Western Blotting (WB), Immunocytochemistry (IC), Immunohistochemistry (IH) and Flow Cytometry (2 ug per 10^6 cells). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:100 - 1:500 is recommended for IC and IH. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Neurofilaments contain three intermediate filament proteins: light (68 kDa), medium (160 kDa) and heavy (200 kDa). Neurofilament heavy (NF200 or NF-H) is phosphorylated and it is thought that this results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized with 5% trehalose
Storage Temp:
After reconstitution of lyophilised antibody, aliquot and store at -20 °C for a higher stability. Avoid freeze-thaw cycles.
Host Animal:
Mouse
Species Reactivity:
Chicken, Rat, Other Mammals (Predicted)
Immunogen:
Full length native protein (purified) from Pig spinal cord.
Western Blotting (WB), Immunocytochemistry (IC) and Flow Cytometry (2 ug/10^6 cells). Suggested dilution for WB of 1:5,000-10,000. This antibody recognises NF-H in frozen sections, tissue culture and in formalin-fixed sections. Suggested dilution for IC is 1:500-1,000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
NF-200; NF200; NF-H; NEFH; N52; Neurofilament heavy polypeptide; Neurofilament triplet H protein; 200 kDa neurofilament protein; KIAA0845; NFH;
Accession Number:
P12036 NFH_HUMAN;
Reconstitution:
Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material.
Shelf Life:
12 months after purchase
Specificity:
The specificity of this antibody has been confirmed by WB. This antibody recognises phosphorylated NF-H KSP (lysine-serine-proline) type sequences. In some species there is some cross-reactivity with the related KSP sequences found in subunit NF-M. | Chicken, Rat. Predicted to react with mammals due to sequence homology.
References:
1. Boylan K. et al (2009) Immunoreactivity of the phosphorylated axonal neurofilament H subunit (pNF-H) in blood of ALS model rodents and ALS patients: evaluation of blood pNF-H as a potential ALS biomarker. J Neurochem. 2009 Dec;111(5):1182-91.2. Rangaraju S. et al (2009) Molecular architecture of myelinated peripheral nerves is supported by calorie restriction with aging. Aging Cell. 2009 Apr;8(2):178-91.
Neurofilaments contain three intermediate filament proteins: light (68 kDa), medium (160 kDa) and heavy (200 kDa). Neurofilament heavy (NF200 or NF-H) is phosphorylated and it is thought that this results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
After reconstitution of lyophilised antibody, aliquot and store at -20 °C for a higher stability. Avoid freeze-thaw cycles.
Host Animal:
Rabbit
Species Reactivity:
Rat, Other Mammals (Predicted)
Immunogen:
Purified bovine NF-H. Bovine intermediate filaments were prepared from spinal cords and the cytoskeletal material was dissolved in 6M urea. Individual neurofilament subunits were purified by ion exchange chromatography on DEAE cellulose followed by preparative gel electrophoresis.
Western Blotting (WB), Immunocytochemistry (ICC) and Immunocytochemistry (IHC). Suggested dilution for WB of 1:5,000-10,000. Suggested dilution for ICC/IHC is 1:1,000-1:5,000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
NF-200; NF200; NF-H; NEFH; N52; Neurofilament heavy polypeptide; Neurofilament triplet H protein; 200 kDa neurofilament protein; KIAA0845; NFH;
Accession Number:
P12036 NFH_HUMAN;
Reconstitution:
Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material.
Shelf Life:
12 months after purchase
Specificity:
The specificity of this antibody has been confirmed by WB. This antibody reacts with phosphorylated NF-H and is seen as a band of approx 200 kDa. | Rat. Predicted to react with other mammals due to sequence homology.
Neurofilaments contain three intermediate filament proteins: light (68 kDa), medium (160 kDa) and heavy (200 kDa). Neurofilament heavy (NF200 or NF-H) is phosphorylated and it is thought that this results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
After reconstitution of lyophilised antibody, aliquot and store at -20 °C for a higher stability. Avoid freeze-thaw cycles.
Host Animal:
Rabbit
Species Reactivity:
Rat, Other Mammals (Predicted)
Immunogen:
Purified rat NF-H construct containing most of the tandem KSP repeats expressed and purified from E.coli.
Western Blotting (WB) and Immunocytochemistry (IC). Suggested dilution for WB of 1:5,000-10,000. Suggested dilution for IC is 1:500-1,000. This antibody stains dendritic and perikaryal neurofilaments particularly well. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
NF-200; NF200; NF-H; NEFH; N52; Neurofilament heavy polypeptide; Neurofilament triplet H protein; 200 kDa neurofilament protein; KIAA0845; NFH;
Accession Number:
P12036 NFH_HUMAN;
Reconstitution:
Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material.
Shelf Life:
12 months after purchase
Specificity:
The specificity of this antibody has been confirmed by WB. This antibody reacts with noth phosphorylated NF-H at approx 200 kDa and non-phosphorylated NF-H at 160 kDa. | Rat. Predicted to react with other mammals due to sequence homology.
Neurofilaments are composed of three intermediate filament proteins: light (~68 kDa), medium (~160 kDa) and heavy (~200 kDa), which are involved in the maintenance of the neuronal caliber. Neurofilament light (NF68 or NF-L) is the most abundant of the three proteins.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
After reconstitution of lyophilised antibody, aliquot and store at -20 °C for a higher stability. Avoid freeze-thaw cycles.
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A dilution of 1:10,000 - 1:20,000 is recommended for WB. A dilution of 1:2,000 - 1:5,000 is recommended for ICC/IHC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Neurofilaments are composed of three intermediate filament proteins: light (~68 kDa), medium (~160 kDa) and heavy (~200 kDa), which are involved in the maintenance of the neuronal caliber. Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
After reconstitution of lyophilised antibody, aliquot and store at -20 °C for a higher stability. Avoid freeze-thaw cycles.
Host Animal:
Rabbit
Species Reactivity:
Hu, Rat, Ms, Fel, Chk
Immunogen:
A recombinant fusion protein containing the extreme C-terminus of rat NF-M expressed in and purified from E. coli.
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A dilution of 1:1,000 - 1:5,000 is recommended for WB, ICC and IHC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Neurofilament medium polypeptide; NF-M; 160 kDa neurofilament protein; Neurofilament 3; Neurofilament triplet M protein; Nefm; Nef3; Nfm;
Accession Number:
P12839 NFM_RAT;
Reconstitution:
Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material.
Shelf Life:
12 months after purchase
Specificity:
Specifically recognizes the medium neurofilament subunit NF-L in WB. Band appears at ~145 kDa in WB from rodent and ~160 kDa for human and bovine WB. | Hu, Rat, Ms, Fel, Chk
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