Background Info: |
Plastocyanin (PC) is a small Cu protein and a mobile electron carrier in the lumen of the thylkoids. PC interacts with the B/F complex and Photosystem I. Alternative name: DNA-damage-repair/toleration protein DRT112. |
Product Type: |
Antibody |
Antibody Type: |
Polyclonal |
Format: |
Lyophilized |
Storage Temp: |
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
Host Animal: |
Rabbit |
Species Reactivity: |
Arabidops thaliana, Brassica juncea, Heliantus annuus, Hordeum vulgare, Lathyrus sativus, Nicotiana tabacum, Oryza sativa, Pisum sativum, Spinacia oleracea, Solanum tuberosum, Synechocystis sp. PCC6803, Zea mays |
Expected Species: |
Catalpa bungei, Dicots, Chlamydomonas reinhardtii, Nicotiana benthamiana, Physcomitrium patens, Ricinus communis, Solanum lycopersicumSpecies of your interest not listed? Contact us |
Immunogen: |
Purified native plastocyanin from Spinacia oleracea UniProt: P00289 |
Applications: |
Immunogold (IG), Western blot (WB) |
Data Sheet: |
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Additional Info: |
Plastocyanin runs abberant due to negative charge at 12-19 kDa on SDS-PAGE depending upon the system used. in 15 % gel the protein will run closer to its true MW than in 12 % gel. In some cases PC can be very acidic and run at twice of its MW.PC1 runs closer to 14 kDa while PC2 runs closer to 19 kDa. For good resolution adding fresh DTT to the sample buffer is recommended. PC2 is generally more abundant and it increases with Cu feeding. PC1 is expressed first after etiolated seedlings are placed in the light. |
Application Details: |
1 : 100 (IG), 1 : 2000 (WB) |
Purity: |
Serum |
Reconstitution: |
For reconstitution add 50 l of sterile water |
Molecular Weight: |
10 kDa |
Not reactive in: |
No confirmed exceptions from predicted reactivity are currently known |
Selected references: |
Tokarz et al. (2021). Stem Photosynthesis-A Key Element of Grass Pea (Lathyrus sativus L.) Acclimatisation to Salinity. Int J Mol Sci. 2021 Jan 12;22(2):685. doi: 10.3390/ijms22020685. PMID: 33445673; PMCID: PMC7828162.Viola et al. (2021) In vivo electron donation from plastocyanin and cytochrome c6 to PSI in Synechocystis sp. PCC6803. Biochim Biophys Acta Bioenerg. 2021 May 15;1862(9):148449. doi: 10.1016/j.bbabio.2021.148449. Epub ahead of print. PMID: 34004195.Furutani et al. (2021) The difficulty of estimating the electron transport rate at photosystem I. J Plant Res. 2021 Nov 15. doi: 10.1007/s10265-021-01357-6. Epub ahead of print. PMID: 34778922.Wang et al. (2020) Rerouting of ribosomal proteins into splicing in plant organelles. BioRxiv, DOI: 10.1101/2020.03.03.974766 .Galvis et al. (2020). H+ transport by K+ EXCHANGE ANTIPORTER3 promotes photosynthesis and growth in chloroplast ATP synthase mutants. Plant Physiol. pp.01561.2019. doi: 10.1104/pp.19.01561. |
Special application note: |
Cellular [compartment marker] of chloroplast thylakoid lumenThis product can be sold containing ProClin if requested. |
UniProt number: |
P00289 |
TAIR number: |
AT1G20340 |
Research area: |
Electron transfer |
Code: |
Photo20 CM20 |