| Background Info: |
ATP synthase is the universal enzyme that stnthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient.This product is a recombinant protein standard, source: Synechocystis strain PCC 6803. |
| Product Type: |
Antibody |
| Format: |
Lyophilized, in glycerol. |
| Storage Temp: |
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
| Applications: |
Western blot (WB) |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Additional Info: |
Concentration: after adding 90 l of dest. water final concentration of the standard is 0.27 pmol/ l.Protein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing. |
| Application Details: |
Standard curve: 3 loads are recommended (0.5, 2 and 4μl).For most applications a sample load of 0.2μg of chlorophyll will give a AtpB signal in this range.Positive control: load per well: a 2μl load is optimal for most chemiluminescent detection systems.This standard is stabilized and ready and does not require heating before loading on the gel.Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing. |
| Reconstitution: |
For reconstitution add 90 l of milliQ water, Please notice that this product contains 10% glycerol and might appear as liquid but is provided lyophilized |
| Molecular Weight: |
in most gel systems AtpB migrates around 50-54 kDa |
| Selected references: |
Fraser et al. (2013). Photophysiological and Photosynthetic Complex Changes during Iron Starvation in Synechocystis sp. PCC 6803 and Synechococcus elongatus PCC 7942. PLOS ONE. |
| Special application note: |
The AtpB protein standard can be used in combination with global anti-AtpB antibodies to quantitate AtpB from a wide range of species. Global antibodies are raised against highly conserved amino acid sequences in the AtpB protein.Quantitative western blot: detailed method description, video tutorial |
| Research area: |
Photosynthesis protein standards-quantitation |
| Code: |
Photo20 |
|
| Background Info: |
ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. |
| Product Type: |
Antibody |
| Antibody Type: |
Polyclonal |
| Format: |
Liquid at 23,3 g/ l |
| Storage Temp: |
Store at 2-8°C.; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
| Host Animal: |
Chicken |
| Species Reactivity: |
Arabidopsis thaliana, Hordeum vulgare, Phaeodactylum tricornutum, Spartina alterniflora, Spinacia oleracea, Synechocystis PCC 6803, Synechococcus PCC 7942, beef muscle, Rat liver |
| Expected Species: |
Acinetobacter baumannii, Bacillus subtilis, Brassica napus, Cannabis sativa, Clostridioides difficile, Cyanobacteria, E.coli K-12, Galdieria sulphuraria, Heliomicrobium modesticaldum Ice1, Manihot esculenta, Nicotiana plumbaginifolia, Saccharomyces cerevisiae, Salmonella typhimurium, Trichodesmium erythraeum, Triticum aestivum, Vitis vinifera, Zosteria marina, Yrsinia sp.Species of your interest not listed? Contact us |
| Immunogen: |
KLH-conjugated synthetic peptide derived from available plant, algal (chloroplastic and mitochondrial) and bacterial sequences of beta subunits of F-type ATP synthases, including Arabidopsis thaliana chloroplastic ATP synthase subunit beta UniProt: P19366, TAIR: AtCg00480 and Arabidopsis thaliana mitochondrial ATP synthase subunit beta-1, UniProt: P83483, TAIR: At5g08670 as well as Chlamydomonas reinhardtii, UniProt: P06541 and A8IQU3 |
| Applications: |
Immunolocalization (IL), Immunogold (IG), Western blot (WB) |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Additional Info: |
Results of immunogold studies using anti-AtpB antibody are published in Andersson et al. (2009). |
| Application Details: |
1 : 500 for localization of native enzyme by immunogold (IL), 1 : 5 000-1 : 8 000 (WB) |
| Purity: |
Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide. |
| Molecular Weight: |
53.9 kDa (Arabidopsis thaliana), 51.7 kDa (Synechocystis PCC 6803), 53.7 kDa (Spinacia oleracea) |
| Not reactive in: |
Archeal V-type ATP synthase |
| Selected references: |
Neusius et al. (2022) Lysine acetylation regulates moonlighting activity of the E2 subunit of the chloroplast pyruvate dehydrogenase complex in Chlamydomonas. Plant J. 2022 Sep;111(6):1780-1800. doi: 10.1111/tpj.15924. Epub 2022 Aug 8. PMID: 35899410.Levitan et al. (2019). Structural and functional analyses of photosystem II in the marine diatom Phaeodactylum tricornutum. Proc Natl Acad Sci U S A. 2019 Aug 27;116(35):17316-17322. doi: 10.1073/pnas.1906726116.Nelson et al. (2019). Protein lysine methylation contributes to modulating the response of sensitive and tolerant Arabidopsis species to cadmium stress. doi: 10.1111/pce.13692.Gellert et al. (2018). A single point mutation on the cucumber mosaic virus surface induces an unexpected and strong interaction with the F1 complex of the ATP synthase in Nicotiana clevelandii plants. Virus Res. 2018 Jun 2;251:47-55. doi: 10.1016/j.virusres.2018.05.005. (immunogold)Quesada et al. (2011). Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development. Plant J. Nov;68(4):738-53. doi: 10.1111/j.1365-313X.2011.04726.x. Epub 2011 Sep 13. |
| Special application note: |
The anti-AtpB antibody will detect the mitochondrial form of the F1 ATP synthase subcomplex, as well as the chloroplastic CF1 ATP synthase and most known bacterial F-type ATP synthases, Peptide used for antibody production is located in a beta sheet, which is partly exposed near the surface of the AtpB protein |
| UniProt number: |
P19366 , P83483 , P06541 , A8IQU3 |
| TAIR number: |
AT5G08670, ATCG00480 |
| Research area: |
Global Antibodies |
| Code: |
Photo20 |
|
