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Product Search Results: AS20 4485

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Product Search Results: AS20 4485

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Agrisera AS20 4485 100 µg £254.00
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Background Info: Fluorescent proteins, like EBFP, can be used as protein "tags" to study the subcellular localization of proteins and/or their translocation upon stimulation and/or as markers for transfections in transient and stable expression systems.
Product Type: Antibody
Antibody Type: Monoclonal
Format: Lyophilized
Storage Temp: Store lyophilized/reconstituted at 2-8 C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal: Mouse
Species Reactivity: BFP, GFP, YFP
Immunogen: Recombinant EBFP (NCBI accession number AX_766758 REGION: 1-717, expression vector pGEX-1N), expressed in E.coli.
Applications: ELISA (ELISA), Western blot (WB)
Data Sheet: PLEASE CLICK HERE TO DOWNLOAD
Application Details: 1 : 150-1: 3000 (ELISA), 1 : 500-1 : 5000 (WB)
Conjugation: IgG1
Isotype: IgG1
Purity: Immunoglobulin Protein A purified in a 10 mM ammonium bicarbonate buffer, with 2 mg of BSA.
Reconstitution: Recommended antibody concentration: 0.5 mg/ml (when dissolved at 0.5 mg/ml, the BSA concentration will be 1%). Recommended solvent; 100 mM PBS or Tris-HCl, pH 7.0 •Additional sodium azide ( up to 0.05%) is recommended for long term storage. •For a 0.5 mg/ml antibody concentration in 1% BSA, dissolve in 200 μl buffer.
Research area: GFP/EGFP/BFP/CFP/YFP/RFP