Dynactin subunit 1 (DCTN1) is the largest (approximately 150 kD) subunit of dynactin, a macromolecular complex consisting of multiple subunits ranging in from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein and is involved in a diverse range of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis.
DCTN1 contains N-terminal CAP-Gly and basic domains, followed by the coiled-coil (CC) 1 and 2 domains [1]. The CAP-Gly and basic domains form the microtubule binding domain (MTBD) [2] and the CC1 and CC2 domains mediate the interactions with dynein intermediate chain (DIC) and the other dynactin subunits [1]. The MTBDs of DCTN1 allow microtubule binding during spindle formation and chromosome movement making them essential for cell division[3],
Mutations in the DCTN1 gene are associated with amyotrophic lateral sclerosis (ALS) and distal hereditary motor neuronopathy type VIIB (HMN7B/spinal and bulbar muscular atrophy/dSBMA).
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal
Format:
100 µg in 100 µl PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Temp:
Store at -20°C. Avoid freeze / thaw cycles.
Host Animal:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Expected Species:
Guinea Pig, Sheep, Goat, Golden Hamster, Cat, Pig
If you would like us to check if this antibody is likely to bind to this protein from a different species please contact us. We are happy to check for you.
Immunogen:
Partial length recombinant human DCTN1 from the C-terminal region
If you would like further information regarding the immunogen used in the production of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
142kDa (Intended as a general guide and does not allow for all isoforms and species variations)
Subcellular location:
Cytoplasm cytoskeleton
Purification:
Affinity purification
References:
1: Schroer TA. Dynactin. Annu Rev Cell Dev Biol. 2004;20:75979. 2: Culver-Hanlon TL, Lex SA, Stephens AD, Quintyne NJ, King SJ. A microtubule-binding domain in dynactin increases dynein processivity by skating along microtubules. Nat Cell Biol. 2006;8(3):26470. 3: Robinson RW, Snyder JA. Colocalization studies of Arp1 and p150Glued to spindle microtubules during mitosis: the effect of cytochalasin on the organization of microtubules and motor proteins in PtK1 cells. Cell Biol Int. 2006;30(7):6319.
Dynactin subunit 1 (DCTN1) is the largest (approximately 150 kD) subunit of dynactin, a macromolecular complex consisting of multiple subunits ranging in from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein and is involved in a diverse range of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis.
DCTN1 contains N-terminal CAP-Gly and basic domains, followed by the coiled-coil (CC) 1 and 2 domains [1]. The CAP-Gly and basic domains form the microtubule binding domain (MTBD) [2] and the CC1 and CC2 domains mediate the interactions with dynein intermediate chain (DIC) and the other dynactin subunits [1]. The MTBDs of DCTN1 allow microtubule binding during spindle formation and chromosome movement making them essential for cell division[3],
Mutations in the DCTN1 gene are associated with amyotrophic lateral sclerosis (ALS) and distal hereditary motor neuronopathy type VIIB (HMN7B/spinal and bulbar muscular atrophy/dSBMA).
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal
Format:
50 µg in 50 µl PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Temp:
Store at -20°C. Avoid freeze / thaw cycles.
Host Animal:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Expected Species:
Guinea Pig, Sheep, Goat, Golden Hamster, Cat, Pig
If you would like us to check if this antibody is likely to bind to this protein from a different species please contact us. We are happy to check for you.
Immunogen:
Partial length recombinant human DCTN1 from the C-terminal region
If you would like further information regarding the immunogen used in the production of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
142kDa (Intended as a general guide and does not allow for all isoforms and species variations)
Subcellular location:
Cytoplasm cytoskeleton
Purification:
Affinity purification
References:
1: Schroer TA. Dynactin. Annu Rev Cell Dev Biol. 2004;20:75979. 2: Culver-Hanlon TL, Lex SA, Stephens AD, Quintyne NJ, King SJ. A microtubule-binding domain in dynactin increases dynein processivity by skating along microtubules. Nat Cell Biol. 2006;8(3):26470. 3: Robinson RW, Snyder JA. Colocalization studies of Arp1 and p150Glued to spindle microtubules during mitosis: the effect of cytochalasin on the organization of microtubules and motor proteins in PtK1 cells. Cell Biol Int. 2006;30(7):6319.
Dynactin subunit 1 (DCTN1) is the largest (approximately 150 kD) subunit of dynactin, a macromolecular complex consisting of multiple subunits ranging in from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein and is involved in a diverse range of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis.
DCTN1 contains N-terminal CAP-Gly and basic domains, followed by the coiled-coil (CC) 1 and 2 domains [1]. The CAP-Gly and basic domains form the microtubule binding domain (MTBD) [2] and the CC1 and CC2 domains mediate the interactions with dynein intermediate chain (DIC) and the other dynactin subunits [1]. The MTBDs of DCTN1 allow microtubule binding during spindle formation and chromosome movement making them essential for cell division[3],
Mutations in the DCTN1 gene are associated with amyotrophic lateral sclerosis (ALS) and distal hereditary motor neuronopathy type VIIB (HMN7B/spinal and bulbar muscular atrophy/dSBMA).
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal
Format:
100 µg in 100 µl PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
Storage Temp:
Store at -20°C. Avoid freeze / thaw cycles.
Host Animal:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Expected Species:
If you would like us to check if this antibody is likely to bind to this protein from a different species please contact us. We are happy to check for you.
If you would like further information regarding the immunogen used in the production of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
142kDa (Intended as a general guide and does not allow for all isoforms and species variations)
Subcellular location:
Cytoplasm
Purification:
Affinity purification
References:
1: Schroer TA. Dynactin. Annu Rev Cell Dev Biol. 2004;20:75979. 2: Culver-Hanlon TL, Lex SA, Stephens AD, Quintyne NJ, King SJ. A microtubule-binding domain in dynactin increases dynein processivity by skating along microtubules. Nat Cell Biol. 2006;8(3):26470. 3: Robinson RW, Snyder JA. Colocalization studies of Arp1 and p150Glued to spindle microtubules during mitosis: the effect of cytochalasin on the organization of microtubules and motor proteins in PtK1 cells. Cell Biol Int. 2006;30(7):6319.
Dynactin subunit 1 (DCTN1) is the largest (approximately 150 kD) subunit of dynactin, a macromolecular complex consisting of multiple subunits ranging in from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein and is involved in a diverse range of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis.
DCTN1 contains N-terminal CAP-Gly and basic domains, followed by the coiled-coil (CC) 1 and 2 domains [1]. The CAP-Gly and basic domains form the microtubule binding domain (MTBD) [2] and the CC1 and CC2 domains mediate the interactions with dynein intermediate chain (DIC) and the other dynactin subunits [1]. The MTBDs of DCTN1 allow microtubule binding during spindle formation and chromosome movement making them essential for cell division[3],
Mutations in the DCTN1 gene are associated with amyotrophic lateral sclerosis (ALS) and distal hereditary motor neuronopathy type VIIB (HMN7B/spinal and bulbar muscular atrophy/dSBMA).
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal
Format:
50 µg in 50 µl PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
Storage Temp:
Store at -20°C. Avoid freeze / thaw cycles.
Host Animal:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Expected Species:
If you would like us to check if this antibody is likely to bind to this protein from a different species please contact us. We are happy to check for you.
If you would like further information regarding the immunogen used in the production of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
142kDa (Intended as a general guide and does not allow for all isoforms and species variations)
Subcellular location:
Cytoplasm
Purification:
Affinity purification
References:
1: Schroer TA. Dynactin. Annu Rev Cell Dev Biol. 2004;20:75979. 2: Culver-Hanlon TL, Lex SA, Stephens AD, Quintyne NJ, King SJ. A microtubule-binding domain in dynactin increases dynein processivity by skating along microtubules. Nat Cell Biol. 2006;8(3):26470. 3: Robinson RW, Snyder JA. Colocalization studies of Arp1 and p150Glued to spindle microtubules during mitosis: the effect of cytochalasin on the organization of microtubules and motor proteins in PtK1 cells. Cell Biol Int. 2006;30(7):6319.
Plays a key role in dynein-mediated retrograde transport of vesicles and organelles along microtubules by recruiting and tethering dynein to microtubules. Binds to both dynein and microtubules providing a link between specific cargos, microtubules and dynein. Essential for targeting dynein to microtubule plus ends, recruiting dynein to membranous cargos and enhancing dynein processivity (the ability to move along a microtubule for a long distance without falling off the track). Can also act as a brake to slow the dynein motor during motility along the microtubule (PubMed:25185702). Can regulate microtubule stability by promoting microtubule formation, nucleation and polymerization and by inhibiting microtubule catastrophe in neurons. Inhibits microtubule catastrophe by binding both to microtubules and to tubulin, leading to enhanced microtubule stability along the axon (PubMed:23874158). Plays a role in metaphase spindle orientation (PubMed:22327364). Plays a role in centriole cohesion and subdistal appendage organization and function. Its recruitement to the centriole in a KIF3A-dependent manner is essential for the maintenance of centriole cohesion and the formation of subdistal appendage. Also required for microtubule anchoring at the mother centriole (PubMed:23386061). Plays a role in primary cilia formation (PubMed:25774020).
DCTN1 Antibody detects endogenous levels of total DCTN1
Immunogen:
A synthesized peptide derived from human DCTN1
Cross Reactivity:
Human,Mouse,Rat
Conjugate:
Unconjugated
purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink(TM) Coupling Resin (Thermo Fisher Scientific).
Concentration:
1mg/ml
Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt
Plays a key role in dynein-mediated retrograde transport of vesicles and organelles along microtubules by recruiting and tethering dynein to microtubules. Binds to both dynein and microtubules providing a link between specific cargos, microtubules and dynein. Essential for targeting dynein to microtubule plus ends, recruiting dynein to membranous cargos and enhancing dynein processivity (the ability to move along a microtubule for a long distance without falling off the track). Can also act as a brake to slow the dynein motor during motility along the microtubule (PubMed:25185702). Can regulate microtubule stability by promoting microtubule formation, nucleation and polymerization and by inhibiting microtubule catastrophe in neurons. Inhibits microtubule catastrophe by binding both to microtubules and to tubulin, leading to enhanced microtubule stability along the axon (PubMed:23874158). Plays a role in metaphase spindle orientation (PubMed:22327364). Plays a role in centriole cohesion and subdistal appendage organization and function. Its recruitement to the centriole in a KIF3A-dependent manner is essential for the maintenance of centriole cohesion and the formation of subdistal appendage. Also required for microtubule anchoring at the mother centriole (PubMed:23386061). Plays a role in primary cilia formation (PubMed:25774020).
DCTN1 Antibody detects endogenous levels of total DCTN1
Immunogen:
A synthesized peptide derived from human DCTN1
Cross Reactivity:
Human,Mouse,Rat
Conjugate:
Unconjugated
purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink(TM) Coupling Resin (Thermo Fisher Scientific).
Concentration:
1mg/ml
Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt
Gene ID:
1639
Gene:
DCTN1
Swiss-Prot:
Q14203
Molecular Weight:
142kDa
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