The NEXTflex Rapid Pre-Capture Combo Kit is a complete low-input library prep solution designed for DNA library preparation, multiplexing, and barcode blocking, upstream of SeqCap EZ Library target enrichment. This kit facilitates deep multiplexing for both exome target capture and DNA target capture.
The NEXTflex Rapid PreCapture Combo Kit is designed to prepare single read or paired-end libraries for DNA and exome target capture and Illumina sequencing. This kit features Enhanced Adapter Ligation Technology, resulting in library preps with a larger number of unique sequencing reads.
[BENEFITS]
Complete kit Contains library prep, barcodes and barcode blockers compatible with SeqCap EZ target capture Low input - only 10 ng of input DNA required Optimized - Offers larger number of on-target reads Multiplex up to 96 samples with single-index barcodes Reduces the cost of target capture Compatible with Illumina sequencing platforms
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
[Kit Specs] The NEXTflex Rapid Pre-Capture Combo Kit contains DNA library preparation reagents to prepare 48, 96 or 192 genomic DNA or exome libraries for SeqCap EZ target capture and downstream Illumina sequencing. The shelf life of all reagents is 12 months when stored properly at -20°C. This kit is shipped on dry ice.
[Automation for Library Prep for Target Capture] The NEXTflex Rapid Pre-Capture Combo Kit is compatible with Beckman Biomek® FX and Biomek® FXP Laboratory Automation Workstations. A program is currently being validated for use with the Sciclone NGS, Sciclone NGSx and Sciclone G3 platforms.
[Multiplex up to 96 Samples with SeqCap Target Capture] The NEXTflex DNA Barcodes and NEXTflex DNA Barcode Blockers for SeqCap are included in the NEXTflex Rapid Pre-Capture Combo Kit and are also available separately.
Our whitepaper written in conjunction with Roche (please email us for a copy), An Integrated Solution to Simplify Library Preparation and Multiplexing for NimbleGen Sequence Capture, demonstrates the efficacy of this kit when used with the NimbleGen SeqCap EZ Library system for library prep in preparation for Illumina sequencing.
[Achieving High Coverage and Yield from GC and AT Rich Genomes] The PCR amplification step in library preparation introduces biases including uneven coverage of regions with extreme base composition, increased numbers of duplicate fragments, decreased mapping quality and poor variant calling. In our new white paper, Achieving High Coverage and Yield from GC and AT Rich Genomes, we show that the NEXTflex PCR polymerase contains a highly optimized and robust enzyme that exhibits minimal GC bias and produces uniform coverage of difficult to sequence genomes.
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 5144-41
The NEXTflex Rapid Pre-Capture Combo Kit is a complete low-input library prep solution designed for DNA library preparation, multiplexing, and barcode blocking, upstream of SeqCap EZ Library target enrichment. This kit facilitates deep multiplexing for both exome target capture and DNA target capture.
The NEXTflex Rapid PreCapture Combo Kit is designed to prepare single read or paired-end libraries for DNA and exome target capture and Illumina sequencing. This kit features Enhanced Adapter Ligation Technology, resulting in library preps with a larger number of unique sequencing reads.
[BENEFITS]
Complete kit Contains library prep, barcodes and barcode blockers compatible with SeqCap EZ target capture Low input - only 10 ng of input DNA required Optimized - Offers larger number of on-target reads Multiplex up to 96 samples with single-index barcodes Reduces the cost of target capture Compatible with Illumina sequencing platforms
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
[Kit Specs] The NEXTflex Rapid Pre-Capture Combo Kit contains DNA library preparation reagents to prepare 48, 96 or 192 genomic DNA or exome libraries for SeqCap EZ target capture and downstream Illumina sequencing. The shelf life of all reagents is 12 months when stored properly at -20°C. This kit is shipped on dry ice.
[Automation for Library Prep for Target Capture] The NEXTflex Rapid Pre-Capture Combo Kit is compatible with Beckman Biomek® FX and Biomek® FXP Laboratory Automation Workstations. A program is currently being validated for use with the Sciclone NGS, Sciclone NGSx and Sciclone G3 platforms.
[Multiplex up to 96 Samples with SeqCap Target Capture] The NEXTflex DNA Barcodes and NEXTflex DNA Barcode Blockers for SeqCap are included in the NEXTflex Rapid Pre-Capture Combo Kit and are also available separately.
Our whitepaper written in conjunction with Roche (please email us for a copy), An Integrated Solution to Simplify Library Preparation and Multiplexing for NimbleGen Sequence Capture, demonstrates the efficacy of this kit when used with the NimbleGen SeqCap EZ Library system for library prep in preparation for Illumina sequencing.
[Achieving High Coverage and Yield from GC and AT Rich Genomes] The PCR amplification step in library preparation introduces biases including uneven coverage of regions with extreme base composition, increased numbers of duplicate fragments, decreased mapping quality and poor variant calling. In our new white paper, Achieving High Coverage and Yield from GC and AT Rich Genomes, we show that the NEXTflex PCR polymerase contains a highly optimized and robust enzyme that exhibits minimal GC bias and produces uniform coverage of difficult to sequence genomes.
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 5144-42
The NEXTflex Rapid Pre-Capture Combo Kit is a complete low-input library prep solution designed for DNA library preparation, multiplexing, and barcode blocking, upstream of SeqCap EZ Library target enrichment. This kit facilitates deep multiplexing for both exome target capture and DNA target capture.
The NEXTflex Rapid PreCapture Combo Kit is designed to prepare single read or paired-end libraries for DNA and exome target capture and Illumina sequencing. This kit features Enhanced Adapter Ligation Technology, resulting in library preps with a larger number of unique sequencing reads.
[BENEFITS]
Complete kit Contains library prep, barcodes and barcode blockers compatible with SeqCap EZ target capture Low input - only 10 ng of input DNA required Optimized - Offers larger number of on-target reads Multiplex up to 96 samples with single-index barcodes Reduces the cost of target capture Compatible with Illumina sequencing platforms
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
[Kit Specs] The NEXTflex Rapid Pre-Capture Combo Kit contains DNA library preparation reagents to prepare 48, 96 or 192 genomic DNA or exome libraries for SeqCap EZ target capture and downstream Illumina sequencing. The shelf life of all reagents is 12 months when stored properly at -20°C. This kit is shipped on dry ice.
[Automation for Library Prep for Target Capture] The NEXTflex Rapid Pre-Capture Combo Kit is compatible with Beckman Biomek® FX and Biomek® FXP Laboratory Automation Workstations. A program is currently being validated for use with the Sciclone NGS, Sciclone NGSx and Sciclone G3 platforms.
[Multiplex up to 96 Samples with SeqCap Target Capture] The NEXTflex DNA Barcodes and NEXTflex DNA Barcode Blockers for SeqCap are included in the NEXTflex Rapid Pre-Capture Combo Kit and are also available separately.
Our whitepaper written in conjunction with Roche (please email us for a copy), An Integrated Solution to Simplify Library Preparation and Multiplexing for NimbleGen Sequence Capture, demonstrates the efficacy of this kit when used with the NimbleGen SeqCap EZ Library system for library prep in preparation for Illumina sequencing.
[Achieving High Coverage and Yield from GC and AT Rich Genomes] The PCR amplification step in library preparation introduces biases including uneven coverage of regions with extreme base composition, increased numbers of duplicate fragments, decreased mapping quality and poor variant calling. In our new white paper, Achieving High Coverage and Yield from GC and AT Rich Genomes, we show that the NEXTflex PCR polymerase contains a highly optimized and robust enzyme that exhibits minimal GC bias and produces uniform coverage of difficult to sequence genomes.
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 5144-43
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