| Product Detail: |
Sheep anti-Alpha-synuclein Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded, ELISA. |
| Background Info: |
Alpha synuclein is an abundant 140 amino acid neuronal protein, expressed primarily at presynaptic terminals in the central nervous system. Alpha synuclein has been associated with several neurodegenerative diseases. A point mutation in the gene coding for the alpha-synuclein protein was the first discovery linking this protein to a rare familial form of Parkinson's disease (PD). Subsequently, other mutations in the alpha-synuclein gene have been identified in familial PD. The aggregated proteinaceous inclusions called Lewy bodies found in PD and cortical Lewy body dementia (LBD) were discovered to be predominantly alpha-synuclein. Aberrant aggregation of alpha-synuclein has been detected in an increasing number of neurodegenerative diseases, collectively known as synucleopathies. Alpha-synuclein exists physiologically in both soluble and membrane-bound states, in unstructured and alpha-helical conformations, respectively. The physiological function of alpha-synuclein appears to require its translocation between these subcellular compartments and interconversion between the 2 conformations. Abnormal processing of alpha-synuclein is predicted to lead to pathological changes in its binding properties and function. |
| Product Type: |
Antibody |
| Antibody Type: |
Polyclonal |
| Format: |
Lyophilized |
| Host Animal: |
Sheep |
| Species Reactivity: |
Human,Mouse,Rat |
| Immunogen: |
A synthetic peptide (CMPVDPDNEAYEMPSEE) of human alpha synuclein protein (aa: 116-131) conjugated to diphtheria toxoid has been used as the immunogen. |
| Applications: |
ELISA,IHC-Frozen,IHC-Paraffin-embedded,WB |
| Antibody Isotype: |
IgG |
| Application Details: |
Immunohistochemistry (IHC): 1-4 µg/mL (paraffin sections) Western Blotting (WB): 0.5 - 2.0 µg/mL. Fixing of proteins on membrane with 0.4% formaldehyde (30 min at room temperature) recommended, see Lee & Kamitani, 2011. Flow Cytometry: 2 µg antibody per ~10^6 cells, methanol-fixed. Immunocytochemistry (ICC): 1-4 µg/mL, 4% formaldehyde-fixed cells. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Alternative Names: |
Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACP |
| Biosensis Brand: |
Biosensis® |
| Conjugate: |
Unconjugated |
| Shelf Life: |
12 months after date of receipt (unopened vial). |
| Use: |
For research use only. |
| References: |
Lundvig et al, http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15790525'>Brain Res Mol Brain Res 134, 3-17 (Mar 24, 2005). Bennett, http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15737408'>Pharmacol Ther 105, 311-31 (Mar, 2005). Vekrellis, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15247485'>Mol Neurobiol 30, 1-21 (Aug, 2004). Martin, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15182224'>Protein Pept Lett 11, 229-37 (Jun, 2004). Doherty, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=14648076'>Acta Neuropathol (Berl) 107, 169-75 (Feb, 2004). Goedert, http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11377973'>Curr Opin Genet Dev 11, 343-51 (Jun, 2001). Saito, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=10967182'>J Neurol Sci 177, 48-59 (Aug 1, 2000). Luecking C.B. et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids= 11215516'>Cell Mol Life Sci. 2000 Dec;57(13-14):1894-908. Kahle, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11193173'>Ann N Y Acad Sci 920, 33-41 (2000). Clayton, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9641537'>Trends Neurosci 21, 249-54 (Jun, 1998). |
| Specificity: |
This antiserum specifically detects alpha synuclein. This antibody is known to react with alpha synuclein of human, mouse, rat and other rodents. |
| Purification: |
Affinity purified and dialysed against phosphate buffered saline (PBS). |
| Target: |
Alpha-synuclein |
| Accession Number: |
P37840 |
| Research Areas: |
Antibodies & Conjugates/Biosensis Alzheimer's & Parkinson's/sheep antibody to alpha synuclein |
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