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Product Search Results: S-075-50

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Product Search Results: S-075-50

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Biosensis S-075-50 50 µg £270.00
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Product Detail: Sheep anti-Alpha-synuclein Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded, ELISA.
Background Info: Alpha synuclein is an abundant 140 amino acid neuronal protein, expressed primarily at presynaptic terminals in the central nervous system. Alpha synuclein has been associated with several neurodegenerative diseases. A point mutation in the gene coding for the alpha-synuclein protein was the first discovery linking this protein to a rare familial form of Parkinson's disease (PD). Subsequently, other mutations in the alpha-synuclein gene have been identified in familial PD. The aggregated proteinaceous inclusions called Lewy bodies found in PD and cortical Lewy body dementia (LBD) were discovered to be predominantly alpha-synuclein. Aberrant aggregation of alpha-synuclein has been detected in an increasing number of neurodegenerative diseases, collectively known as synucleopathies. Alpha-synuclein exists physiologically in both soluble and membrane-bound states, in unstructured and alpha-helical conformations, respectively. The physiological function of alpha-synuclein appears to require its translocation between these subcellular compartments and interconversion between the 2 conformations. Abnormal processing of alpha-synuclein is predicted to lead to pathological changes in its binding properties and function.
Product Type: Antibody
Antibody Type: Polyclonal
Format: Lyophilized
Host Animal: Sheep
Species Reactivity: Human,Mouse,Rat
Immunogen: A synthetic peptide (CMPVDPDNEAYEMPSEE) of human alpha synuclein protein (aa: 116-131) conjugated to diphtheria toxoid has been used as the immunogen.
Applications: ELISA,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype: IgG
Application Details: Immunohistochemistry (IHC): 1-4 µg/mL (paraffin sections)Western Blotting (WB): 0.5 - 2.0 µg/mL. Fixing of proteins on membrane with 0.4% formaldehyde (30 min at room temperature) recommended, see Lee & Kamitani, 2011.Flow Cytometry: 2 µg antibody per ~10^6 cells, methanol-fixed.Immunocytochemistry (ICC): 1-4 µg/mL, 4% formaldehyde-fixed cells.Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names: Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACP
Accession Number: P37840 (SYUA_HUMAN)
Reconstitution: Reconstitute in 50 µL of sterile water. Centrifuge to remove any insoluble material.
Storage: After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Shelf Life: 12 months after date of receipt (unopened vial).
Specificity: This antiserum specifically detects alpha synuclein. This antibody is known to react with alpha synuclein of human, mouse, rat and other rodents.
References: Kuo Y.M. et al. (2010) Extensive enteric nervous system abnormalities in mice transgenic for artificial chromosomes containing Parkinson disease-associated alpha-synuclein gene mutations precede central nervous system changes. Hum Mol Genet. May 1;19(9):1633-50Pelkonen A. et al. (2010) Stimulated dopamine overflow and alpha-synuclein expression in the nucleus accumbens core distinguish rats bred for differential ethanol preference. J Neurochem. 2010 Aug;114(4):1168-76.Alves et al. (2008) Striatal and nigral pathology in a lentiviral rat model of Machado-Joseph disease Hum Mol Genet. 2008 Jul 15;17(14):2071-83.
Research Areas: Biosensis Alzheimer's & Parkinson's
Purity: Affinity purified and dialysed against phosphate buffered saline (PBS).
Conjugate: Unconjugated