Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Human IgA, (α chain)
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (α) chains on human IgA
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin human serum immunoglobulins · light chains on all human immunoglobulins · serum proteins from bovine, mouse or rabbit
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Rabbit
Immunogen:
Purified Goat IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.8, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on goat IgG · light chains on all goat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin goat serum immunoglobulins
Country Of Origin:
Rabbit serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Human IgG, whole molecule
Buffer:
30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on human IgG · light chains on all human immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin human serum immunoglobulins · serum proteins from bovine, mouse or rabbit
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Rabbit IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.1% (v/v) Kathon CG
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rabbit IgG · light chains on all rabbit immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rabbit serum proteins · human serum proteins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Human IgA, (α chain)
Buffer:
PBS, 1% BSA & 0.1% proclin 150
Preservative:
0.1% (v/v) Kathon CG
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.
Specificity:
Based on IEP, this antibody reacts with: · heavy (α) chains on human IgA
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin human serum proteins · light chains on all human immunoglobulins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Rabbit
Immunogen:
Purified Mouse IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.8, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum proteins
Country Of Origin:
Rabbit serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Human IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.8, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on human IgG · light chains on all human immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin human serum immunoglobulins · serum proteins from bovine, mouse or rabbit
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Donkey
Immunogen:
Purified Rat IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.8, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rat IgG · light chains on all rat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rat serum proteins
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Host:
Goat
Immunogen:
Purified Human IgG, Fc fragment
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on human IgG
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin human serum immunoglobulins · light chains on all human immunoglobulins · human IgG F(ab)2 fragment · serum proteins from bovine, horse, mouse, or rabbit
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on goat IgG · light chains on all goat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin goat serum immunoglobulins
Country Of Origin:
Chicken Ig fraction was prepared using serum from healthy hens of US origin.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Human IgG, Fc fragment
Buffer:
30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on human IgG
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin human serum immunoglobulins · light chains on all human immunoglobulins · human IgA or IgM
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Host:
Goat
Immunogen:
Purified Human IgG, Fc fragment
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on human IgG
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin human serum immunoglobulins · light chains on all human immunoglobulins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum immunoglobulins
Country Of Origin:
Chicken Ig fraction was prepared using serum from healthy hens of US origin.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Donkey
Immunogen:
Purified Goat IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.8, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on goat IgG · light chains on all goat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin goat serum immunoglobulins
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Donkey
Immunogen:
Purified Goat IgG, whole molecule
Buffer:
30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on goat IgG · light chains on all goat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: non-immunoglobulin goat serum immunoglobulins
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rabbit IgG · light chains on all rabbit immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rabbit serum proteins
Country Of Origin:
Chicken Ig fraction was prepared using serum from healthy hens of US origin.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified using solid phase Human IgA + IgG + IgM
Purity:
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Host:
Goat
Immunogen:
Purified human IgG + IgA + IgM, whole molecule
Buffer:
30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy chains on human IgA + IgG + IgM · light chains on all human immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin human serum immunoglobulins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Donkey
Immunogen:
Purified Rat IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.8, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rat IgG · light chains on all rat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rat serum proteins · IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit or sheep
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rat IgG · light chains on all rat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rat serum proteins · serum proteins from human or rabbit · IgG from human or rabbit
Country Of Origin:
Chicken Ig fraction was prepared using serum from healthy hens of US origin.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Donkey
Immunogen:
Purified Rat IgG, whole molecule
Buffer:
30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rat IgG · light chains on all rat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rat serum proteins
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rat IgG · light chains on all rat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rat serum proteins
Country Of Origin:
Chicken Ig fraction was prepared using serum from healthy hens of US origin.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Chicken
Immunogen:
Purified Rabbit IgG, whole molecule
Buffer:
30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rabbit IgG · light chains on all rabbit immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rabbit serum proteins · IgG from human or mouse
Country Of Origin:
Chicken Ig fraction was prepared using serum from healthy hens of US origin.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Donkey
Immunogen:
Purified Goat IgG, whole molecule
Buffer:
30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on goat IgG · light chains on all goat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin goat serum immunoglobulins · IgG from human, mouse, rabbit or rat
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Host:
Goat
Immunogen:
Purified Mouse IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum immunoglobulins · human IgG or serum proteins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Donkey
Immunogen:
Purified Mouse IgG, whole molecule
Buffer:
30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum immunoglobulins
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Donkey
Immunogen:
Purified Goat IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on goat IgG · light chains on all goat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin goat serum immunoglobulins · IgG from human, mouse, rabbit or rat
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Chicken
Immunogen:
Purified Goat IgG, whole molecule
Buffer:
30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on goat IgG · light chains on all goat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin goat serum immunoglobulins · IgG/serum proteins from human, mouse or rabbit
Country Of Origin:
Chicken Ig fraction was prepared using serum from healthy hens of US origin.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Human IgG, Fc fragment
Buffer:
30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on human IgG
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin human serum immunoglobulins · light chains on all human immunoglobulins · human IgG F(ab)2 fragment · serum proteins from bovine, horse, mouse, or rabbit
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Donkey
Immunogen:
Purified Mouse IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.8, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum immunoglobulins
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Human IgG, Fc fragment
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.8, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on human IgG
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin human serum immunoglobulins · light chains on all human immunoglobulins · human IgG F(ab)2 fragment · serum proteins from bovine, horse, mouse, or rabbit
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Host:
Goat
Immunogen:
Purified Human IgM, µ chain
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Specificity:
Based on IEP, this antibody reacts with: · heavy (µ) chains on human IgM
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin human serum proteins · light chains on all human immunoglobulins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Mouse IgG, whole molecule
Buffer:
30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum immunoglobulins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Donkey
Immunogen:
Purified Sheep IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on sheep IgG · light chains on all sheep immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin sheep serum proteins · IgG from human, mouse or rabbit
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Donkey
Immunogen:
Purified Mouse IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.8, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum immunoglobulins · IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Donkey
Immunogen:
Purified Sheep IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.8, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on sheep IgG · light chains on all sheep immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin sheep serum proteins · IgG from human, mouse or rabbit
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Host:
Goat
Immunogen:
Purified Human IgG, Fc fragment
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.8, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on human IgG
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin human serum immunoglobulins · light chains on all human immunoglobulins · human IgG F(ab)2 fragment · serum proteins from bovine, horse, mouse, or rabbit
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Donkey
Immunogen:
Purified Mouse IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum immunoglobulins · IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Donkey
Immunogen:
Purified Rabbit IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.8, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rabbit IgG · light chains on all rabbit immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rabbit serum proteins
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Donkey
Immunogen:
Purified Sheep IgG, whole molecule
Buffer:
30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on sheep IgG · light chains on all sheep immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin sheep serum proteins
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Donkey
Immunogen:
Purified Rabbit IgG, whole molecule
Buffer:
30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rabbit IgG · light chains on all rabbit immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rabbit serum proteins
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Donkey
Immunogen:
Purified Sheep IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.8, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on sheep IgG · light chains on all sheep immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin sheep serum proteins
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Mouse IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum immunoglobulins · human IgG or serum proteins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
VSV-G tag corresponds to the partial peptide sequence of the vesicular stomatitis virus glycoprotein from Rhabdoviridae family, and consists of a single RNA molecule that encodes five proteins, one of which is a surface glycoprotein (G).. This epitope tag can be added to a protein from any species to allow further protein detection using different techniques like: immunolocalization, immunoprecipitation or Western blot.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid in PBS
Storage Temp:
Store at -20 °C; Avoid repeated freeze/thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes. For long term storage, transfer to -80 C
Peanut Ara h1 is a major peanut allergen. It shows significant homology with the vicilin seed storage protein found in most higher plants.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arachis hypogaea
Immunogen:
Recombinant peanut allergen Ara h1, UniProt: P43237, amino acid 26-216.
Omega-gliadin is wheat allergen. It is a water-insoluble component of gluten. Omega-gliadin is one of the three main types of gliadin to which body in intolerant in celiac disease.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C or -80 C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Triticum monococcum
Expected Species:
Aegilops tauschiiSpecies of your interest not listed? Contact us
Nucleaoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (human) plays a fundamental role during virion assembly through packaging of the positive strand viral genome RNA into a helical ribonucleocapsid (RNP).Alternative names: NC, Protein N, Nucleocapsid protein
Product Type:
Antibody
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C/-80°C. Reconstitute in deionized sterile water to a concentration from 0.1-1.0 mg/ml and add 5-50% of glycerol (final concentration). 50 % glycerol is a default final recommended concentration. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Working aliquotes can be stored at 4℃ for up to one week.Shelf life of liquid form is 6 monthss at -20 °C/-80°C. The shelf life of lyophilized form is 12 months at -20 °C/-80°C.
N-terminal His tagged Nucleoprotein (N) (6xHis) was overexpressed in E.coli in the region 1-419 amino acids UniProt P0DTC9: MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTAAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA Purity: >90 % as confirmed by SDS-PAGEBuffer: 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Wheat gliadin is a class of proteins being a main component of gluten fraction and present in wheat and other cereals.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C or -80 C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Major allergen Alt a 1 (allergen Alt a 1), ALTA1 is a unique beta-barrel protein dimer found in fungi, Alternaria, most common mold, associated with allergic diseses like asthma.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C or -80 C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Major pollen allergen Dac g 4 (allergen Dac g 4) (Fragments) is a grass pollen allergen.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C or -80 C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Pollen allergen Dac g 3 with other names: Allergen Dac g III, allergen Dac g 3.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C or -80 C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Goat anti-Rabbit IgG Fc, ALP conjugated, min. cross-reactivity to bovine horse or human serum proteins is a secondary antibody, conjugated to ALP, which binds to rabbit IgG (H&L), Fc in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store liquid at Store at 2-8°C. For storage at -20 C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Rabbit IgG .Antibody is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on rabbit IgG .Based on IEP, no reactivity is observed to: • non-immunoglobulin rabbit serum proteins • light chains on all rabbit immunoglobulins • Rabbit IgG F(ab)'2 fragment • serum proteins from bovine, horse or human .
Major allergen Api g 1, isoallergen 2 (Allergen Api g 1.0201) (allergen Api g 1)
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C or -80 C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Apium graveolens
Immunogen:
Recombinant Apium graveolens Major allergen Api g 1, isoallergen 2 protein, amino acids: 1-159, UniProt:P92918
Osmotin protein (from thaumatin protein family), coded by a gene AP24. Known to inhibit the germination and growth of the fungus Phytophthora infestans. Protein is induced by: salt stress, ABA viral infection and wounding. Localised to vacuole.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C or -80 C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Nicotiana tabacum
Expected Species:
Capsicum annuum, Solanum lycopersicumSpecies of your interest not listed? Contact us
Immunogen:
Recombinant Osmotin derived from Nicotiana tabacum protein sequence, amino acids: 22-246. UniProt: P14170
>95%, Protein G purified to a total immunoglobulin G fraction.
Molecular Weight:
26 | 27 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Preservative: 0.03% Proclin 300. Preparation contains: 50% Glycerol, 10 mM PBS, pH 7.4Reactivity of this antibody on endogenous sample remains to be determined.
L-ascorbate oxidase has oxidoreductase activity and belongs to multicopper oxidase family and is an apoplastic enzyme involved in metabolism of plant ascorbate (AA). Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in photosynthetic tissues. Over 90% of the ascorbate is localized in the cytoplasm, but a substantial proportion is exported to the apoplast.Alternative names: (ASO) (Ascorbase) (EC 1.10.3.3), AAO
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C or -80 C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Cucurbita maxima
Expected Species:
Cucumis melo, Cucumis sativus, Nelumbo nucifera, Theobroma cacao Species of your interest not listed? Contact us
Reactivity of this antibody on endogenous material remains to be determined
Application Details:
1 : 1000 - 1: 5000 (WB)
Purity:
>95%, Protein G purified to a total immunoglobulin G fraction.
Molecular Weight:
65 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Preservative: 0.03% Proclin 300. Preparation contains: 50% Glycerol, 10 mM PBS, pH 7.4Reactivity of this antibody on endogenous material remains to be determined.
ACC synthase 8 belongs to a group fo enzymes which catalyze the conversion of S-adenosyl-L-methionine (SAM) into 1-aminocyclopropane-1-carboxylate (ACC), a direct precursor of ethylene. ACS8 is auxin inducible. Alternative names: 1-aminocyclopropane-1-carboxylate synthase 8, ACC synthase 8, S-adenosyl-L-methionine methylthioadenosine-lyase 8.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C or -80 C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Rabbit IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on rabbit IgG .Based on IEP, no reactivity is observed to:• non-immunoglobulin rabbit serum proteins • light chains on all rabbit immunoglobulins • rabbit IgG F(ab)'2 fragment • serum proteins from bovine, horse or human
OLE9 | Major pollen allergen Ole e 9 is an enzyme (EC 3.2.1.39), which is catalyzing Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.Alternative names: Glucan endo-1,3-beta-D-glucosidase (Major pollen allergen Ole e 9) (allergen Ole e 9), OLE9
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C or -80 C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Polygalacturonase (PG) is an enzyme (EC 3.2.1.15) involved in cell wall biogenesis and degradation and fruit ripening. Protein is cusing an allergic reaction in human. Alternative names: Allergen Cry j II, Major pollen allergen Cry j 2, Pectinase, allergen Cry j 2.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C or -80 C, avoid repeated freeze-thaw cycles.
Gamma-glutamyl hydrolase is an enzyme (EC 3.4.19.9) displaying gamma-glutamyl-peptidase activity. Alternative names: conjugase) (GH) (Gamma-Glu-X carboxypeptidase)
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C or -80 C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Glycine max
Expected Species:
Glycine soja (Gamma-glutamyl hydrolase isoform A and B) Species of your interest not listed? Contact us
Major pollen allergen Lol p 5a causes an allergic reaction in human - grass pollen ellergy. Alternative names: Allergen Lol p Ib, Allergen Lol p Va, allergen Lol p 5a.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C or -80 C, avoid repeated freeze-thaw cycles.Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Lolium perenne
Immunogen:
Recombinant Lolium perenne Major pollen allergen Lol p 5a protein amino acid: 26-307, UniProt: Q40240
Cyclin-B1-1 is a cyclin-dependent protein kinase involved in cell division. Functions as an effector of growth contol at G2/M. Alternative names: Cyc1-At, G2/mitotic-specific cyclin-B1-1, CycB1;1, CYCB1-1, CYC1
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C or -80 C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica campestris, Capsella rubella, Eutrema salsugineum, Noccaea caerulescensSpecies of your interest not listed? Contact us
Immunogen:
Recombinant Cyclin-B1-1 protein derived from Arabidopsis thaliana sequence, amino acids: 1-428. UniProt: P30183 TAIR: AT4G37490
>95%, Protein G purified to a total immunoglobulin G fraction.
Molecular Weight:
48,4 | 49 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Preservative: 0.03% Proclin 300. Preparation contains: 50% Glycerol, 10 mM PBS, pH 7.4Reactivity of this antibody on endogenous extract remains to be confirmed.
Conglutin (allergen Ara h 6) causes allergic reaction in humans and binds to IgE.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C or -80 C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Parvalbumin beta causes allergic reaction in humans. Alternative names: Allergen Gad c I, Allergen M
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C or -80 C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Gadus morhua subsp. callarias (Baltic cod) (Gadus callarias)
Goat anti-Mouse IgG Fc, ALP conjugated, min. cross-reactivity to bovine, horse or human serum proteins is a secondary antibody conjugated to APL, which binds to mouse IgG Fc in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store non-diluted antibody at 2-8°C. For storage at -20 C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Mouse IgG .Antibody is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on mouse IgG .Based on IEP, no reactivity is observed to: • non-immunoglobulin mouse serum proteins • light chains on all mouse immunoglobulins • mouse IgG, F(ab)'2 fragment • serum proteins from bovine, horse, or human .
Putative protease Do-like 12 is a mitochondrial enzyme with serine-type endopeptidase avtivity (EC 3.4.21.-) involved in proteolysis. Localized to mitochondrial matrix. Alternative names: Degradation of Periplasmic Proteins 12.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C or -80 C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Immunogen:
Recombinant Putative protease Do-like 12 derived from the sequence of Arabidopsis thaliana, amino acids 25-499, UniProt: Q9LK70 TAIR: AT3G16550
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Preservative: 0.03% Proclin 300. Preparation contains: 50% Glycerol, 10 mM PBS, pH 7.4Reactivit of this antibody on endogenous material remains to be determined.
Polygalacturonase (PG) is an enzyme (EC 3.2.1.15) involved in cell wall biogenesis/degradation and food ripening. Belongs to glycoside hydrolase family 28. Alternative names: Pectinase, allergen Cha o 2.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C or -80 C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Hamster IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on hamster IgG • light chains on all hamster immunoglobulins .Based on IEP, no reactivity is observed to: • non-immunoglobulin hamster serum immunoglobulins .
Arachin Ahy-3 is a hexamer, composed of an acidic and a basic chain, derived from a single precursor and linked by a disulfied bond. Belongs to the 11S seed storage protein (globulins) family. Arachin Ahy-3 chain alpha; Arachin Ahy-3 chain beta.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C or -80 C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Indole 3 acetic acid (IAA) is the principal auxin in higher plants. This hormone is produced in in cells in the apex and young leaves of a plant. Plant cells synthesize IAA from tryptophan. Different effects caused by auxines include: induction of cell elongation and cell division and have a subsequent results for plant growth and development.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Freeze upon arrival and store at -20 °C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Antibody can be used for direct detection of free IAA (see reference below)Antibody is provided in 50% glycerol. For larger quantity (1 mg)- please inquire.Steedman's wax embedding technique is recommended to be used with this antibody. The most critical issue is to keep the temperature below 37 C during the whole embedding procedure. More information can be found in Vitha et al. (2000).
Application Details:
Specific information about dilution is going to be included on the vial
Purity:
Total IgG. Protein G purified in PBS.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Nukazuka et al. (2021). A Role for Auxin in Triggering Lamina Outgrowth of Unifacial Leaves. Plant Physiol. 2021 Feb 23:kiab087. doi: 10.1093/plphys/kiab087. Epub ahead of print. PMID: 33620494.Dinis et al. (2018). Kaolin modulates ABA and IAA dynamics and physiology of grapevine under Mediterranean summer stress. J Plant Physiol. 2018 Jan;220:181-192. doi: 10.1016/j.jplph.2017.11.007.Escand n et al. (2016). Integrated physiological and hormonal profile of heat-induced thermotolerance in Pinus radiata. Tree Physiol. 2016 Jan;36(1):63-77. doi: 10.1093/treephys/tpv127. Epub 2016 Jan 12.Jesus et al. (2015). Salicylic acid application modulates physiological and hormonal changes in Eucalyptus globulus under water deficit. Environ and Exp Botany, Volume 118, October 2015, Pages 56?66.De Diego et al. (2013).Immunolocalization ofIAA andABA inroots andneedles ofradiatapine (Pinusradiata) duringdrought andrewatering. Tree Physiol., May; 33(5):537-549.
Goat anti-human IgA + IgG + IgM is a secondary antibody, which binds to human IgA, IgG and IgM and is FITC (fluorescein-5-isothiocyanate) conjugated. Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are affinity purified using solid phase human IgG + IgA + IgM.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy and light chains on human IgA, IgG and IgM based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Cytokinins (CK) belong to a class of plant growth substances (plant hormones) which promote cell deivision by means of local and long distance signalling. Zeatin is an adenine-type cytokinin synthesised in stems, leaves and roots. Synonym: 9-(β-D-Ribofuranosyl)-trans-zeatin, N6-(trans-4-Hydroxy-3-methyl-2-buten-1-yl)adenosine
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Dihydrozeatin riboside
Expected Species:
Dihydrozeatin riboside
Immunogen:
BSA-conjugated, via ribose, dihydrozeatin riboside
Cytokinins (CK) belong to a class of plant growth substances (plant hormones) which promote cell deivision by means of local and long distance signalling. Zeatin is an adenine-type cytokinin synthesised in stems, leaves and roots. Synonym: 9-(?-D-Ribofuranosyl)-trans-zeatin, N6-(trans-4-Hydroxy-3-methyl-2-buten-1-yl)adenosine
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
2-10 l/15 ml; Specific information about dilution is going to be included on the vial
Purity:
Total IgG. Protein G purified in PBS.
Reconstitution:
For reconstitution add 70 l of sterile water
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Ferreira et al. (2019). Enzyme-mediated metabolism in nutritive tissues of galls induced by Ditylenchus gallaeformans (Nematoda: Anguinidae). Plant Biol (Stuttg). 2019 May 18. doi: 10.1111/plb.13009.
Cytokinins (CK) belong to a class of plant growth substances (plant hormones) which promote cell deivision by means of local and long distance signalling. Zeatin is an adenine-type cytokinin synthesised in stems, leaves and roots.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Cytokinins (CK) belong to a class of plant growth substances (plant hormones) which promote cell deivision by means of local and long distance signalling. Zeatin is an adenine-type cytokinin synthesised in stems, leaves and roots. Synonym: 9-(β-D-Ribofuranosyl)-trans-zeatin, N6-(trans-4-Hydroxy-3-methyl-2-buten-1-yl)adenosine
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Cytokinins (CK) belong to a class of plant growth substances (plant hormones) which promote cell deivision by means of local and long distance signalling.Zeatin is an adenine-type cytokinin synthesised in stems, leaves and roots.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For 1 mg of antibodies For ELISA and immunoassay or For ELISA kit - please inquire, Antibodies are supplied in 50% glycerol,This antibody cannot discriminate between the zeatin ribozide and zeatin, Zeatin cross-reactivity is usually lower ca,50%,BSA can be used For blocking using this antibody
Application Details:
Specific information about dilution is going to be included on the vial
Purity:
Total IgG. Protein G purified in PBS.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Han et al. (2019). Characterization and T-DNA insertion sites identification of a multiple-branches mutant br in Betula platyphylla and Betula pendula. BMC Plant Biol. 2019 Nov 12;19(1):491. doi: 10.1186/s12870-019-2098-y..
Cytokinins (CK) belong to a class of plant growth substances (plant hormones) which promote cell deivision by means of local and long distance signalling. Zeatin is an adenine-type cytokinin synthesised in stems, leaves and roots.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Cytokinins (CK) belong to a class of plant growth substances (plant hormones) which promote cell deivision by means of local and long distance signalling. Zeatin is an adenine-type cytokinin synthesised in stems, leaves and roots.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Cytokinins (CK) belong to a class of plant growth substances (plant hormones) which promote cell deivision by means of local and long distance signalling. Zeatin is an adenine-type cytokinin synthesised in stems, leaves and roots. Synonym: 9-(?-D-Ribofuranosyl)-trans-zeatin, N6-(trans-4-Hydroxy-3-methyl-2-buten-1-yl)adenosine
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Bowie et al. (2018). N6-Furfuryladenine is protective in Huntington's disease models by signaling huntingtin phosphorylation. Proc Natl Acad Sci U S A. 2018 Jul 24;115(30):E7081-E7090. doi: 10.1073/pnas.1801772115.
Cytokinins (CK) belong to a class of plant growth substances (plant hormones) which promote cell deivision by means of local and long distance signalling.Zeatin is an adenine-type cytokinin synthesised in stems, leaves and roots.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Specific information about dilution is going to be included on the vial
Purity:
Total IgG. Protein G purified in PBS.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Alvarez et al. (2020). Hormonal and gene dynamics in de novo shoot meristem formation during adventitious caulogenesis in cotyledons of Pinus pinea. Plant Cell Rep. 2020 Jan 28. doi: 10.1007/s00299-020-02508-0.
Cytokinins (CK) belong to a class of plant growth substances (plant hormones) which promote cell deivision by means of local and long distance signalling.Zeatin is an adenine-type cytokinin synthesised in stems, leaves and roots.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Ortho-topolin riboside
Expected Species:
Ortho-topolin riboside
Immunogen:
BSA-conjugated, via ribose, ortho-topolin riboside
Cytokinins (CK) belong to a class of plant growth substances (plant hormones) which promote cell deivision by means of local and long distance signalling. Zeatin is an adenine-type cytokinin synthesised in stems, leaves and roots.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Ortho-topolin riboside
Expected Species:
Ortho-topolin riboside
Immunogen:
BSA-conjugated, via ribose, ortho-topolin riboside
Cytokinins (CK) belong to a class of plant growth substances (plant hormones) which promote cell deivision by means of local and long distance signalling.Zeatin is an adenine-type cytokinin synthesised in stems, leaves and roots.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
N6-isopentenyladenosine
Expected Species:
N6-isopentenyladenosine
Immunogen:
BSA-conjugated, via ribose, N6-isopentenyladenosine
This antibody recognize a6A but not m6A or A, which iis caused by the geometrical similarity between isopentenyl and allyl (Shu et al., 2020).
Application Details:
2-10 l/15 ml; Specific information about dilution is going to be included on the vial
Purity:
Total IgG. Protein G purified in PBS.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Shu et al. (2022) m6A-label-seq: A metabolic labeling protocol to detect transcriptome-wide mRNA N6-methyladenosine (m6A) at base resolution, STAR Protocols, Volume 3, Issue 1, 2022, 101096, ISSN 2666-1667, https://doi.org/10.1016/j.xpro.2021.101096.Alvarez et al. (2020). Hormonal and gene dynamics in de novo shoot meristem formation during adventitious caulogenesis in cotyledons of Pinus pinea. Plant Cell Rep. 2020 Jan 28. doi: 10.1007/s00299-020-02508-0.
Cytokinins (CK) belong to a class of plant growth substances (plant hormones) which promote cell deivision by means of local and long distance signalling.Zeatin is an adenine-type cytokinin synthesised in stems, leaves and roots.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Dihydrozeatin riboside
Expected Species:
Dihydrozeatin riboside
Immunogen:
BSA-conjugated, via ribose, dihydrozeatin riboside
For 1 mg of antibodies For ELISA and immunoassay or For ELISA kit - please inquire, Antibodies are supplied in 50% glycerol
Application Details:
Specific information about dilution is going to be included on the vial
Purity:
Total IgG. Protein G purified in PBS.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Alvarez et al. (2020). Hormonal and gene dynamics in de novo shoot meristem formation during adventitious caulogenesis in cotyledons of Pinus pinea. Plant Cell Rep. 2020 Jan 28. doi: 10.1007/s00299-020-02508-0.
Indole 3 acetic acid (IAA) is the principal auxin in higher plants. This hormone is produced in in cells in the apex and young leaves of a plant. Plant cells synthesize IAA from tryptophan. Different effects caused by auxines include: induction of cell elongation and cell division and have a subsequent results for plant growth and development.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
IAA | indole-3-acetic acid (C1')
Expected Species:
IAA | indole-3-acetic acid (C1')
Immunogen:
BSA-conjugated, via C1 carboxyl group, indole-3-acetic acid (C1')
Abscisic acid (ABA) is a plant hormone involved in different physiological responses as stimulation of the closure of stomata (water stress brings about an increase in ABA synthesis), iInhibition of shoot growth, and many others. ABA shown to have both inhibitory as well as many promoting functions.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Abscisic acid (C1)
Expected Species:
Abscisic acid (C1)
Immunogen:
BSA-conjugated abscisic acid (C1) via C1 carboxyl group
Cytokinins (CK) belong to a class of plant growth substances (plant hormones) which promote cell deivision by means of local and long distance signalling.Zeatin is an adenine-type cytokinin synthesised in stems, leaves and roots.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Abscisic acid (ABA) is a plant hormone involved in different physiological responses as stimulation of the closure of stomata (water stress brings about an increase in ABA synthesis), iInhibition of shoot growth, and many others. ABA shown to have both inhibitory as well as many promoting functions.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Abscisic acid (C1) in Arabidopsis thaliana, Eucalyptus globulus, Petunia hybrida L., Pinus radiata, Populus trichocarpa
Expected Species:
Abscisic acid (C1)
Immunogen:
BSA-conjugated abscisic acid (C1) via C1 carboxyl group
The antibody will recognize either the ABA conjugated to glucose ester (ABA-GE) or the ABA precursor: abscisic acid aldehyde, ABA aldehyde is however not usually present in plant tissue similarly to ABA alcohol which is also reactive, The antibodies will predominantly recognize only ABA and its glucosylester
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Total IgG. Protein G purified in PBS with 50 % glycerol.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Wojciechowska et al. (2020). Abscisic Acid and Jasmonate Metabolisms Are Jointly Regulated During Senescence in Roots and Leaves of Populus trichocarpa. Int J Mol Sci , 21 (6) Dinis et al. (2018). Kaolin modulates ABA and IAA dynamics and physiology of grapevine under Mediterranean summer stress. J Plant Physiol. 2018 Jan;220:181-192. doi: 10.1016/j.jplph.2017.11.007. Kovaleva et al. (2017). ABA and IAA control microsporogenesis in Petunia hybrida L. Protoplasma. 2017 Nov 13. doi: 10.1007/s00709-017-1185-x. Escand n et al. (2016). Integrated physiological and hormonal profile of heat-induced thermotolerance in Pinus radiata. Tree Physiol. 2016 Jan;36(1):63-77. doi: 10.1093/treephys/tpv127. Epub 2016 Jan 12.Ondzighi-Assoume et al. (2016). Environmental Nitrate Stimulates Root Tip Abscisic Acid Accumulation via Release from Inactive Stores. Plant Cell. 2016 Feb 17. pii: TPC2015-00946-RA.Jesus et al. (2015). Salicylic acid application modulates physiological and hormonal changes in Eucalyptus globulus under water deficit. Environ and Exp Botany, Volume 118, October 2015, Pages 56–66.Lacuesta et al. (2013). Immunolocalization of IAA and ABA in roots and needles of radiata pine (Pinus radiata) during drought and rewatering. Tree Physiol. May;33(5):537-49.
Cytokinins (CK) belong to a class of plant growth substances (plant hormones) which promote cell deivision by means of local and long distance signalling.Zeatin is an adenine-type cytokinin synthesised in stems, leaves and roots.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
N6-isopentenyladenosine
Expected Species:
N6-isopentenyladenosine
Immunogen:
BSA-conjugated, via ribose, N6-isopentenyladenosine
Indole 3 acetic acid (IAA) is the principal auxin in higher plants. This hormone is produced in in cells in the apex and young leaves of a plant. Plant cells synthesize IAA from tryptophan. Different effects caused by auxines include: induction of cell elongation and cell division and have a subsequent results for plant growth and development
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
indole-3-acetic acid (C1')
Expected Species:
IAA | indole-3-acetic acid (C1')
Immunogen:
BSA- conjugated, via C1 carboxyl group, indole-3-acetic acid (C1')
For 1 mg of antibodies or For ELISA kit - please inquire
Application Details:
to be determined by end user
Purity:
Total IgG. Protein G purified in PBS.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Livanos et al. (2015). Deliberate ROS production and auxin synergistically trigger the asymmetrical division generating the subsidiary cells in Zea mays stomatal complexes. Protoplasma. 2015 Aug 7.
30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (µ) chains on human IgM
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin human serum proteins · light chains on all human immunoglobulins · human IgA or IgG
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
s ECL Plus Western Blotting Substrate is an ultra-sensitive, luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP) at high sensitivity levels (low picogram to mid-femtogram). Western Blotting Substrate may be used for immunoblots, western blots, dot blots and any blotting application utilizing horseradish peroxidase (HRP)-conjugates. The substrate can be used with various blocking buffers and on nitrocellulose or PVDF membranes. Such blots will exhibit low backgrounds. Produced chemiluminescence can be visualized on CCD imaging systems or x-ray film.
Product Type:
Assay & Detection
Storage Temp:
Upon receipt store ECL Plus Western Blotting Substrate at 4°C. Protect from light. It is stable at 4°C for one year.
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilized Exosome Standards from Human Biofluids
Additional Info:
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Tangential flow filtration (TFF) is emerging as one of the most efficient methods for the purification of extracellular vesicles for. TFF-EVs allows a rapid, reproducible and scalable purification of EVs, can be used on the lab bench for purifying small amount of samples (min 5ml) or connected with a mechanical system for purifying larger volumes.
TFF-Easy is a filter cartridge in hollow fibers made of polysulfone, which allows the concentration and the removal of small proteins and molecules from diluted matrices (cell conditioned media, urine, etc.), prior to the EV purification.
The small dimensions of the device allow to concentrate samples from 5 ml up to liters, surmounting the limit of the TFF technique which is usable for processing big volumes of fluids. The filter is additionally suitable for dialyzing EV preparations.
TFF-Easy can be easily washed and it is reusable multiple times.
Application Details:
Applications: Concentration of solutions containing EVs, exosomes or nanoparticles. Concentration of diluted matrices as cell media or urine prior to EV isolation. Removal of small molecules and unbound dyes from solution containing EVs/exosomes and nanoparticles. EV and exosomes dialysis. Concentration of EV suspensions post SEC isolation.
EXO-Prep is a new and efficient method for exosome isolation from biofluids (i.e plasma serum and urine) and from cell culture supernatants. Exosomes are quicky isolated from your sample by an incubation step with EXO-Prep precipitation reagent. Exosome pellet is subsequently collected by one-step centrifugation and can be easily resuspended in PBS 1X. Solubilized exosomes are suitable for different downstream analyses such as proteomic or nucleic acids marker profiling, NTA, electron microscopy etc.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
TFF-MV is a filter cartridge in hollow fibers made of polysulfone. The filter is very useful for separating different EVs by size. Indeed, microvesicles bigger than 150 nm are retained inside the hollow fibers, while small EVs and molecules easily permeate the filter. Microvesicles can be recovered with a syringe in PBS buffer without additional purification steps.
Filter cartridge: Polysulfone hollow fibres
Sample volume per reaction: Recommended sample volume from 10 ml up to several liters if connected to mechanical pump
Application Details:
Applications: Concentration of solutions containing EVs, exosomes or nanoparticles. Concentration of diluted matrices as cell media or urine prior to EV isolation. Removal of small molecules and unbound dyes from solution containing EVs/exosomes and nanoparticles. EV and exosomes dialysis. Concentration of EV suspensions post SEC isolation.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilized Exosome Standards from Human Biofluids
Additional Info:
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilized Exosome Standards from Human Biofluids
Additional Info:
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
MUM1 is a nuclear transcriptional factor (IRF4 or Multiple Myeloma 1 ) and is expressed in final step of intragerminal center B cell differentiation and in post-germinal center B cells. MUM1 is usually mutually exclusive with BCL6 in nonneoplastic tissue. Nuclear expression is present also in a subpopulation of activated T- lymphocytes and expressed in normal and neoplastic melanocytes. In neoplasms MUM1 is found mainly in B-cell lymphoma and melanocytic lesions. In combination with CD138 and Ig´s makes MUM1 more specific marker for differentiating B-cells before plasma cell stage. MUM1 helps to divide diffuse large B cell lymphomas into germinal center (MUM1-) /non-germinal center (MUM1+) phenotypes and helps also to differentiate double hit from Burkitt and DLCL.
E-Cadherin is a 120 kDa transmembrane glycoprotein that is localized in the adherens junctions of epithelial cells. There, it interacts with the cytoskeleton through the associated cytoplasmic catenin proteins. In addition to being a calcium-dependent adhesion molecule, E-Cadherin is also a critical regulator of epithelial junction formation. Its association with catenins is necessary for cell-cell adhesion. These E-cadherin/catenin complexes associate with corical actin bundles at both the zonula adherens and the lateral adhesion plaques. Tyrosine phosphorylation can disrupt these complexes, leading to changes in cell adhesion properties. E-Cadherin expression is often down-regulated in highly invasive, poorly differentiated carcinomas. Increased expression of E-Cadherin in these cells reduces invasiveness. Thus, loss of expression or function of E-Cadherin appears to be an important step in tumorigenic progression.Tissue specificity: Non-neural epithelial tissues.
Programmed cell death ligand 1 (PDL1, CD274) is a type 1 transmembrane protein with role in the regulation of cellular immune responses. PDL1 and its receptor PD-1, interacts and regulating T lymphocyte activation and immune tolerance. Blockade of PD-L1/PD-1 interaction, it enhances the antitumor activity of T lymphocytes. PDL1 is commonly expressed in many tissues and cells, eg. placenta, tonsil and histiocytes. It over expressed in many human tumors such as non-small cell lung carcinoma (NSCLC), melanoma, DLBCL, and different kind of carcinomas. The staining pattern is membranous.
This gene encodes a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds TGFB1 and TGFB3 with high affinity. Mutations in this gene cause hereditary hemorrhagic telangiectasia, also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia.
Phosphohistone H3 (Ser10) (PHH3) is a histone protein, which complexes with the other histones to form the major constituents of chromatin in eukaryotic cells. Phosphorylation of serine 10 amino acid residues in histone H3 occurs only during mitosis late G2 phase. PHH3 is a useful marker for mitoses in several types of tumors and it is useful for identifying mitotic figures in tumors accurately.
Mismatch repair proteins are nuclear enzymes which participate in repair of mismatch errors during DNA replication. Loss of Mismatch repair proteins increases the number of DNA replication errors in the proliferating cells. Errors occur especially in areas of the genome with short repetitive nucleotide sequences - causing microsatellite instability (MSI). MSH6 is a mismatch repair protein which is not expressed in a high proportion of patients with MSI-H. MSH6 antibody can be useful for immunohistochemical analyses of MSH6 protein in neoplastic tissues and identification of loss of MSH6. Immunohistochemical analysis of MSH6 should be performed in IHC panel together with MLH1, MSH2 and PMS2.
MSH2 (MutS Homologue 2) is one of the five key genes (besides MLH1, PMS1, PMS2, MSH6) of the Mis-Match Repair family (MMR). These genes encode MMR proteins, a group of nuclear enzymes that initiates repair of base-base mismatch, that can occur in DNA replication. MMR nuclear proteins form heterodimers, that bind abnormal DNA and initiates its removal. Loss of MMR proteins lead to accumulation of DNA replication errors in the proliferating cells. The above mentioned MMR genes have clinical interest, as they may mutate in families with hereditary non-polyposis colorectal cancer (HNPCC). About 3-5% of all colorectal carcinomas are related to MMR protein mutation. Carriers of an MLH1 or MSH2 mutation have a more than 70% lifetime risk of developing a colorectal carcinoma, with increased risk of developing endometrial carcinomas (50%). Staining for MLH1, MSH2 and MSH6 in colorectal carcinomas should be carried out in patients < 55 years-of-age or with a family history of these tumors.
DNA-mismatch repair (MMR), a conserved process that involves correcting errors made during DNA synthesis, is crucial to the maintenance of genomic integrity. Lack of a functional DNA-mismatch repair pathway is a common characteristic of several different types of human cancers, either due to an MMR gene mutation or promoter-methylation gene silencing. MLH1 is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in hereditary nonpolyposis colon cancer (HNPCC). MLH1 is an integral part of the protein complex responsible for mismatch repair expressed in lymphocytes, heart, colon, breast, lung, spleen, testis, prostate, thyroid and gall bladder, and is methylated in several ovarian tumors. Loss of MLH1 protein expression is associated with a mutated phenotype, microsatellite instability and a predisposition to cancer. In hereditary nonpolyposis colorectal cancer (HNPCC), an autosomal dominant inherited cancer syndrome that signifies a high risk of colorectal and various other types of cancer, the MLH1 gene exhibits a pathogenic mutation. Inactivation of the MLH1 gene causes genome instability and predisposition to cancer. MLH1 also plays a role in meiotic recombination.
Melan-A (MART-1) is a transmembrane protein which is recognized by autologous cytotoxic T lymphocytes. Melan a is expressed in skin melanocytes and melanocyte lineages. This antibody is useful for the identification of melanomas and it should be included into standard melanoma panel for diagnostic. This antibody not cross react with cells of adrenal cortex.
The preproprotein encoded by this gene. Somatostatin is expressed throughout the body and inhibits the release of numerous secondary hormones by binding to high-affinity G-protein-coupled somatostatin receptors. This hormone is an important regulator of the endocrine system through its interactions with pituitary growth hormone, thyroid stimulating hormone, and most hormones of the gastrointestinal tract. Somatostatin also affects rates of neurotransmission in the central nervous system and proliferation of both normal and tumorigenic cells.
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.
The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes.
Mammaglobin is a gene that is expressed almost exclusively in the normal breast epithelium and human breast cancer. It is a member of the secretoglobin gene family and forms a heterodimer with lipophilin B. It has been suggested that mammaglobin may be a useful marker for breast cancer clinical research. Studies investigating the detection of mRNA by RT PCR from circulating carcinoma cells in the peripheral blood of breast cancer patients have shown that mammaglobin is a highly specific marker and correlates with several prognostic factors. Mammaglobin is mammary gland specific and it over expressed in breast cancer.
LI-Cadherin (Cadherin-17, CDH17), is liver-intestinal cadherin and it belongs to the cadherin superfamily. Structure and cellular locations of LI-Cadherin differs from other cadherins like E-CAD, N-CAD or P-CAD. LI-Cadherin is expressed in epithelium of appendix, colon, and intestine and it is not expressed in other normal tissues. LI-Cadherin is positive in carcinomas of colorectal carcinomas and some cases of gastric and pancreas adenocarcinoma.
Cell adhesion molecule with an important role in the development of the nervous system. The L1, neural cell adhesion molecule (L1CAM) plays an important role in axon growth, fasciculation, neural migration and in mediating neuronal differentiation. L1 protein is expressed to tissues arising from neuroectoderm. L1CAM plays also an important role in the malignancy of human tumors and according to several studies, L1CAM positive carcinomas have a bad prognosis. L1CAM is overexpressed in many human carcinomas but it is useful especially in endometrium carcinoma diagnostic.
This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. This protein acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. SOX10 is important and sensitive marker of melanoma especially for spindle cell and desmoplastic melanomas and schwannian neoplasms.
Ki67, also known as MKI67, is aprototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation
SOX2 is a transcription factor which is a member of SRY-related HMG-box (SOX) family. It has a role in the regulation of embryonic development and pluripotency of stem cells. It can be useful especially in lung squamous cell carcinoma diagnostic with panel of other relative markers of squamous carcinoma like P63/P40 and CK5/CK14 for example.
Insulin is a pancreatic hormone that regulates glucose level in blood and it is involved in the synthesis of proteins and fat. Insulin increases cell permeability to monosaccharides, amino acids, and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Insulin is a heterodimer of a B chain and A chain linked by two disulfide bonds. Defects in insulin are the cause of familial hyperproinsulinemia. Insulin is present on the insulin secreted beta cells in islets of Langerhans.
AIB-1 also known as allograft inflammation factor-1 is cytoplasmic actin and calcium binding protein that is expressed especially in activated macrophages and microglia, but also plays part in vascular smooth muscle cell activation and migration. In clinical use IBA-1 can be valuable marker of allograft rejection, macrophages and microglia.
ERBB2: v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian). This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy.Tissue specificity: This protein is expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinomas. EPCAM:Epithelial Cell Adhesion Molecule (EpCAM) is a 40 kDa cell surface antigen. This antigen has been identified independently by a number of groups, and has been known by a variety of names. Several monoclonal antibodies have been raised against EpCAM, many of which have been described as tumour specific molecules on carcinomas. EpCAM is a Type 1 transmembrane glycoprotein. It is expressed on the basolateral membrane of cells by the majority of epithelial tissues, with the exception of adult squamous epithelium and some specific epithelial cell types including hepatocytes and gastric epithelial cells. EpCAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumour cells, and de novo expression being seen in dysplastic squamous epithelium. This cell surface, glycosylated 40kD protein is highly expressed in the bone marrow, colon, lung, and most normal epithelial cells and is expressed on carcinomas of gastrointestinal origin.
ERBB2: v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian). This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized
CD43 (leukosialin, sialophorin) is a transmembrane mucin-like glycoprotein which expressed in plasma membrane especially in T-lymphocytes, some B-cells and cells from myelomonolineage. It is useful for lymphoma diagnostic and it expressed in most T-cell lymphomas and some B-cell lymphomas.
The human leukocyte differentiation antigen CD23 (FCER2) is a key molecule for B-cell activation and growth. It is the low-affinity receptor for IgE. The truncated molecule can be secreted, then functioning as a potent mitogenic growth factor. It is expressed on most mature, conventional B cells (but not on peritoneal CD5+ B cells), and can also be found on the surface of T cells, macrophages, platelets and EBV transformed B lymphoblasts. Expression of CD23 has been detected in neoplastic cells from cases of B cell chronic Lymphocytic leukemia. CD23 is expressed by B cells in the follicular mantle zone but not by proliferating germinal centre cells. CD23 is also expressed by eosinophils. CD23 is distinct from the high affinity IgE receptors found on basophils and mast cells, which mediate allergic reactions. The low affinity receptors are thought to play a role in isotype specific immunoregulation. The regulation of CD23 surface expression appears to be integral with the complex IgE system, which involves interactions of cells, cytokines, antibodies and regulatory factors.
Cytokeratin 14 (CK14) is an acidic type I human intermediate filament protein. It mostly found in basal cells of squamous epithelia, myoepithelium, some glandular epithelia and mesothelial cells. Molecular weight of CK14 is 50 kDa, and it usually pairs with CK5, which is a type II (basic) cytokeratin. In neoplastic cells, CK14 is a useful marker especially in identification of basal cell epithelium in prostate and myoepithelium in breast. It also useful for detecting squamous cell carcinomas. CK5 and CK14 antibodies can be used as a cocktail as well.
B-cell lymphoma/leukaemia-2 (Bcl-2) is an inhibitor of apoptosis, and its expression is generally abundant in cells which dividing and differentiating. In lymphatic tissue, Bcl-2 is highly expressed in T-cells, maturating B cells as well as mature B-cells. However, expression level in germinal center B-cells is downregulated. Overexpression of the Bcl-2 is common in leukemia and various carcinomas and sarcomas. Overexpression is common especially in non-Hodgkins lymphoma. Bcl-2 is helpful to classification of the follicular lymphoma or other lymphomas
CD22 protein may be involved in the localization of B-cells in lymphoid tissues. CD22 is expressed in the cytoplasm and cell membrane of B-cells. CD22 is especially useful in diagnostics of hairy cell leukemia and classification of the B-cell lymphomas.
Cytokeratin 7 (CK7) is a protein that in humans is encoded by the KRT7 gene. CK7 is a member of the keratin family and it is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains co-expressed during differentiation of simple and stratified epithelial tissues. IHC staining of cytokeratin 7 is useful for carcinoma diagnostic especially for differential diagnosis of urothelial, lung, breast carcinomas to colorectal or prostate carcinomas. CK7 is especially marker of lung adenocarcinoma. Pancreas is the good tissue control for CK7.
The androgen receptor (AR), also known as NR3C4 (nuclear receptor subfamily 3, group C, member 4), is a type of nuclear receptor which is activated by binding of either of the androgenic hormones testosterone or dihydrotestosterone in the cytoplasm and then translocating into the nucleus. The androgen receptor is most closely related to the progesterone receptor, and progestins in higher dosages can block the androgen receptor. The main function of the androgen receptor is as a DNA binding transcription factor which regulates gene expression; however, the androgen receptor has other functions as well. Androgen regulated genes are critical for the development and maintenance of the male sexual phenotype.
The CD20 antigen is present on human pre B lymphocytes and on B lymphocytes at all stages of maturation, except on plasma cells. Low level expression of the CD20 antigen has been detected on subpopulation of T lymphocytes. CD20 is expressed widely in the large majority of cases of B-cell leukaemia and lymphoma. The CD20 molecule is involved in regulation of B cell differentiation, presumably via its reported function as a Ca++ channel subunit.
The CD5 antigen is a transmembrane glycoprotein which is expressed on the most mature human T-cells and expression level of CD5 will be increased during T-cell maturation. CD5 is also expressed in a small subset of normal human B-cells as well. CD5 is expressed in most T-cell lymphomas and leukemias and negative expression of the CD5 in T-cell lymphoma indicates a worse prognosis. In B-cell lymphomas eg. small lymphocytic lymphoma, small-cell lymphoma (CD20+), and mantle cell lymphoma are typically CD5 positive and marginal zone lymphoma and follicular lymphoma, are CD5 negative.
The CD4 is membrane glycoprotein (58kDa) and it is highly expressed on human T-helper lymphocytes and thymocytes, as well as at lower levels on cells from monocyte lineage. CD4 is useful marker for recognition of different subtypes of lymphocytes and in diagnostic for T-lymphoblastic lymphomas and histiocytic neoplasia.
AMACR (alpha-methylacyl-CoA racemase) has been recently described as prostate cancer-specific gene that encodes a protein involved in the beta-oxidation of branched chain fatty acids. Expression of AMACR protein is found in prostatic adenocarcinoma but not in benign prostatic tissue. It stains premalignant lesions of prostate: high-grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia. AMACR can be used as a positive marker for PIN. Defects in AMACR are the cause of congenital bile acid synthesis defect type 4 (CBAS4); also known as cholestasis, intrahepatic, with defective conversion of trihydroxycoprostanic acid to cholic acid or trihydroxycoprostanic acid in bile. Clinical features include neonatal jaundice, intrahepatic cholestasis, bile duct deficiency and absence of cholic acid from bile.
CK17, also known as KRT17, it is the type I intermediate filament chain keratin 17. It is found in nail beds, hair follicles, sebaceous glands, and other epidermal appendages. Mutations in this gene lead to Jackson-Lawler type pachyonychia congenita and steatocystoma multiplex. May play a role in the formation and maintenance of various skin appendages, specifically in determining shape and orientation of hair. May be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial "stem cells". May act as an autoantigen in the immunopathogenesis of psoriasis, with certain peptide regions being a major target for autoreactive T-cells and hence causing their proliferation. Required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state. Modulates the function of TNF-alpha in the specific context of hair cycling. Regulates protein synthesis and epithelial cell growth through binding to the adapter protein SFN and by stimulating Akt/mTOR pathway. Involved in tissue repair.
The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
CD2 is a surface antigen of the human T-lymphocyte lineage that is expressed on all peripheral blood T-lymphocytes. It is one of the earliest T-cell markers, being present on more than 95% of thymocytes; it is also found on some natural killer cells but not on B lymphocytes. CD2 antibody is useful for lymphoma diagnostic.
CD7 transmembrane protein is a member of the immunoglobulin superfamily. This protein is found on thymocytes, mature T cells and NK-cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development.
CD8 T cell surface antigen belongs to the type I membrane protein and it is heterodimer of an alpha and a beta chain linked by two disulfide bonds. CD8 positive T-lymphocytes are cytotoxic cells and it thought to play a role in the process of T-cell mediated killing. CD8 antibody is useful for classification of lymphocytes and malignant lymphomas.
CD14 antigen is a GPI-linked glycoprotein with a molecular weight of 55kD. The CD14 antigen is expressed on cells of the myelomonocytic lineage including monocytes, macrophages and Langerhans cells. Low expression is observed on neutrophils and on human B cells. CD14 antigen is a receptor for bacterial lipopolysaccharide (LPS, endotoxin) and the lipopolysaccharide binding protein (LBP). LBP and CD14 antigen serves two physiological roles. These proteins act as opsonin and opsonic receptor, respectively, to promote the phagocytic uptake of bacteria or LPScoated particles by macrophages.
CD45 is transmembrane protein that is present on all differentiated hematopoietic cells (including basophils, granulocytes, lymphocytes, macrophages / histiocytes, mast cells, monocytes, dendritic cells, medullary thymocytes, plasma cells). Erythrocytes and their immediate progenitors do not express CD45 antigen. This antigen is used in routine immunohistochemistry to differentiate between immune cell types, as well as to differentiate hematological malignancies from other tumors. CD 45 is a good marker for AML, ALCL and most B- and T-cell lymphomas. Epithelial tumors, follicular dendritic cell sarcomas, germ cell tumors, melanoma, mesothelioma do not express CD45.
CD13 is a transmembrane protease which expressed widely in different tissues and cells. CD13 expressed especially cells of myeloid origin but also eg. in bile canaliculi of liver, fibroblasts, proximal tubules of kidney, and vascular endothelia. CD13 is useful marker for acute myeloid leukaemia (AML) and differentiating between hepatocellular carcinoma (HCC) and non-hepatocellular tumors.
CD11c is cell surface transmembrane receptor which is mostly expressed on granulocytes, macrophages, monocytes, NK-cells, and some of T- and B-lymphocytes. CD11c is useful especially for diagnosis of hairy cell leukemia (HCL). CD11c can be offer great value for detection panel of HCL with DBA.44, CD103 and other HCL markers
Cytokeratin 19, also known as KRT19, CK19, CK19, K1CS, MGC15366. Entrez Protein NP_002267. It is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis.
CD11b is a membranous protein with a role in adhesive interactions of many leukocytes, especially macrophages, and subsets of lymphocytes. The expression of CD11b increases during maturation and expression levels vary depending on the type of cell. CD11b can be used as common myeloid and NK cell marker. Useful marker for acute promyelocytic leukemia, hair cell leukemia and AML differentiations. Systemic lupus erythematosus is also shown to be associated with CD11b dysfunction.
Cytokeratin 20 (CK20) is expressed in enterocytes and goblet cells of the gastrointestinal (GI) tract. It is also expressed in specific types of simple epithelial cells of the urinary tract. CK20 is useful marker of colorectal carcinoma, gastric, pancreas, urothelium, merkel and biliary system carcinomas.
The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3e is an important pan T-cell marker for the classification of malignant lymphomas and lymphoid leukaemias.
CD50 (ICAM-3) that is expressed in virtually all leukocytes, belongs to the family of the intercellular adhesion molecules. It is also shown to be important part in immune response initiation. CD50 is a useful marker for non-hodgkins lymphoma and it is expressed in almost all the tumors with tendency to be lost in high grade lymphomas.
CD38 is a type II integral membrane glycoprotein which is present on early B and T cell lineages and activated B and T cells but is absent from most mature resting peripheral lymphocytes. CD38 is also found on thymocytes, pre-B cells, germinal center B cells, mitogen-activated T cells, monocytes and Ig-secreting plasma cells. CD38 acts as a NAD glycohydrolase in T lym- phocytes. On hematopoietic cells CD38 induces activation, proliferation, and differentiation of mature T and B cells and mediates apoptosis of myeloid and lymphoid progenitor cells. In addition to acting as a signaling receptor, CD38 is also an enzyme capable of producing several calcium-mobilizing metabo- lites, including cyclic adenosine diphosphate ribose (cADPR). CD38 also plays a role in maintaining survival of an invariant NK T (iNKT) cell subset that preferentially contributes to the maintenance of immunological tolerance.
CD117 is a cell membrane protein encoded by the c-kit proto-oncogene. CD117 is expressed in mast cells, skin melanocytes and interstitial Cajal cells (ICC). These cells show a strong membrane and cytoplasmic staining. CD117 is also expressed in various epithelia (salivary glands, renal tubular cells etc.). Appendix serves as a good positive and negative control tissue. Neoplasms such as gastrointestinal stromal tumor (GIST), mast cell neoplasms and many other (seminoma, Mercel cell carcinoma etc.) express CD117. This antibody (together with DOG-1, CD34, SMA) is of great importance in the diagnosis of GIST, because of specific treatment of GIST patient with Gleveec.
CD10 is a 100kDa glycoprotein, also designated Common Acute Lymphocytic Leukemia Antigen (CALLA). It is a cell surface enzyme with neutral metalloendopeptidase activity which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitts, and follicular germinal center lymphomas, and on cells from patients with chronic myelocytic leukemia (CML). It is also expressed on the surface of normal early lymphoid progenitor cells, immature B cells within adult bone marrow and germinal center B cells within lymphoid tissue. CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells.
Cytokeratin 20 (CK20) is expressed in enterocytes and goblet cells of the gastrointestinal (GI) tract. It is also expressed in specific types of simple epithelial cells of the urinary tract. CK20 is useful marker of colorectal carcinoma, gastric, pancreas, urothelium, merkel and biliary system carcinomas.
Caveolin-1 is a protein which is major structural component of the cell membrane caveolae. Caveolae is structure of the cell membrane invagination. Caveolin-1 is widely expressed in the normal tissue, eg. muscle tissue, vascular endothelia, fibroblasts and adipocytes. Caveolin-1 is useful in lung marker panel, especially in differentiating diagnosis of the epithelioid mesothelioma from lung adenocarcinoma.
Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognising cytokeratins, lymphoid markers, S100, desmin and neurofilaments.
CK5 (keratin 5) is a member of the keratin gene family. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Cytokeratin 5 is expressed in normal basal cells. Mutations of the Cytokeratin5 gene (KRT5) have been shown to result in the autosomal dominant disorderepidermolysis bullosa (EB). Defects in KRT5 are a cause of epidermolysis bullosa simplex.
CD7 transmembrane protein is a member of the immunoglobulin superfamily. This protein is found on thymocytes, mature T cells and NK-cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development.
Cytokeratin 8, also known as CK8, is a member of the low molecular weight type II keratin family. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. CK8 typically dimerizes with CK18 to form an intermediate filament in simple single-layered epithelial cells. It is useful for especially diagnostic of most non-squamous epithelial tumors. squamous tumors are negative for this antibody as a rule.
Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors.
Calretinin is a calcium-binding protein and it is expressed in neurons and in nervous system. Calretinin is also expressed in mesothelial cells and steroid producing cells eg. Leydig cells and adrenal cortical cells as well as fat cells and some neuroendocrine cells. Calretinin located in the cells to nucleus and cytoplasm. Calretinin is useful for mesothelioma diagnostic (differentiate diagnostic between mesothelioma from carcinoma) and it is expressed in most malignant mesothelioma.
Carbonic anhydrase 9 (CA9) is a member of the zinc metalloenzymes that catalyse the reversible hydration of carbon dioxide and is anchored to cell membrane. CA9 is expressed in human gastrointestinal tract, chiefly in stomach, and bile ducts of liver. In neoplasia, high expression levels have been reported in different carcinomas, especially in clear-cell renal cell carcinoma. CA9 is also upregulated in hypoxia.
CD38 is a type II integral membrane glycoprotein which is present on early B and T cell lineages and activated B and T cells but is absent from most mature resting peripheral lymphocytes. CD38 is also found on thymocytes, pre-B cells, germinal center B cells, mitogen-activated T cells, monocytes and Ig-secreting plasma cells. On hematopoietic cells CD38 induces activation, proliferation, and differentiation of mature T and B cells and mediates apoptosis of myeloid and lymphoid progenitor cells. CD38 marker is useful for lymphoma diagnostic eg. using in plasmacytoma diagnostic.
Beta-Catenin is a member of catenin family together with alpha and gamma catenin. It mediates cell-cell adhesion with cadherins and it is key regulatory protein in signaling through the WNT pathway. Beta catenin has a role in cellular proliferation, differentiation and development. Mutations in beta catenin gene (CTNNB1) leads accumulation of the beta catenin protein in cytoplasm and nucleus in different type of tumors eg. endometrial carcinoma and desmoid tumors. This antibody is useful in differentiation diagnostic of tumors.
Cytokeratin 18, also known as CK18, CYK18, KRT18. Entrez Protein NP_000215. It encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.
The CD79 protein is a heterodimer with two CD79a and CD79b phosphoproteins. CD79a is specific for B-cells. The antigen appearing before the pre-B cell stage and it is still expressed at the plasma cell stage. Together with CD20, CD79a is one the most important marker for B-cell neoplasms.
The CD44 antigen, also referred as homing cell adhesion molecule (HCAM), is a multi-structural and multifunctional cell-surface glycoprotein involved in cellcell interactions, cell adhesion, and migration. Most tissues are CD44 positive, including astrocyte restricted precursor cells, breast myoepithelial cells, colon, lung type II pneumocytes, red blood cells, stomach, urothelial basal cells, uterus and white blood cells. Negative staining results is seen in testis, kidney tubular epithelium, cardiac muscle, hepatocytes. In disease, positive staining is seen in colorectal carcinoma (most), Langerhans histiocytosis, oligodendroglioma, thymoma, small cell prostate carcinoma.
This gene encodes a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. This gene encodes the B-cell lineage specific activator protein that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis and so the encoded protein may also play a role in neural development and spermatogenesis. This gene is located at 9p13, which is involved in t(9;14)(p13;q32) translocations recurring in small lymphocytic lymphomas of the plasmacytoid subtype, and in derived large-cell lymphomas. This translocation brings the potent E-mu enhancer of the IgH gene into close proximity of the PAX5 promoter, suggesting that the deregulation of transcription of this gene contributes to the pathogenesis of these lymphomas. Alternatively spliced transcript variants encoding different isoforms have been described but their biological validity has not been determined.
The p63 gene is a homologue of the p53 tumor suppressor gene. Like p53, p63 contains a transactivation (TA) domain induce the transcription of target genes, a DNA binding domain, and an oligomerization domain (OD), used to form tetramers. In contrast to p53, the p63 gene encodes for at least six major isotypes. Three isotypes (TAp63?, TAp63?, and TAp63?) contain the transactivating (TA) domain and are able to transactivate p53 report genes and induce apoptosis. In contrast, the other three isotypes (?Np63?, ?Np63?, ?Np63?) are transcribed from an internal promoter localized within intron3, lack the TA domain, and act as dominant-negatives to suppress transactivation by both p53 and TAp63 isotypes. p63 is highly expressed in the basal cells of the epithelium significant for proper limb outgrowth and morphogenesis.4 In differentiating tissues, p63 is crucial for maintaining the stem cell identity of the basal cells, and is indispensable for correct development of the skin as well as the limb. p63-deficient mice lack all squamous epithelia and their derivatives, including hair, whiskers, teeth, as well as mammary, lacrimal, and salivary glands.Tissue specificity: Widely expressed, notably in heart, kidney, placenta, prostate, skeletal muscle, testis and thymus, although the precise isoform varies according to tissue type. Progenitor cell layers of skin, breast, eye and prostate express high levels of DeltaN-type isoforms. Isoform 10 is predominantly expressed in skin squamous cell carcinomas, but not in normal skin tissues
Thyroid transcription factor, also called thyroid specific enhancer binding nuclear protein (38 kDa), that regulates transcription activity of thyroid (thyroglobulin, thyroid peroxidase, sodium-iodide transport protein, calcitonin and MHC class I) and lung (surfactant proteins A, B and C, Clara cell secretory protein). The expression of TTF1 is confined to follicular epithelial cells and the C-cells in the thyroid, and to the type II pneumocytes and the Clara cells in the lung. In tumor diagnostics TTF1 distinguish primary (TTF+) vs. metastatic (usually TTF1-) lung carcinoma (LCa), pulmonary adenoca (TTF1+) from squamous cell Ca (usually TTF1 -), pleural lung Ca (TTF1+) vs.mesothelioma (TTF1 - ) and pulmonary small cell Ca (TTF1+) vs. Mercel cell Ca (TTF - ). Mucinous lung adeno ca is usually TTF1 negative.
This gene encodes a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. This gene encodes the B-cell lineage specific activator protein that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis and so the encoded protein may also play a role in neural development and spermatogenesis. This gene is located at 9p13, which is involved in t(9;14)(p13;q32) translocations recurring in small lymphocytic lymphomas of the plasmacytoid subtype, and in derived large-cell lymphomas. This translocation brings the potent E-mu enhancer of the IgH gene into close proximity of the PAX5 promoter, suggesting that the deregulation of transcription of this gene contributes to the pathogenesis of these lymphomas. Alternatively spliced transcript variants encoding different isoforms have been described but their biological validity has not been determined.
Napsin A is an aspartic proteinase that is expressed predominantly in lung (type II pneumocytes) and kidney and lower levels in spleen and blood leukocytes. Alveolar macrophages also contain Napsin A due phagosytosis of pneumocytes. Napsin A in useful especially in the differential diagnosis of lung adenocarcinoma between squamous cell carcinoma.
Desmin (DES), with 470-amino acid protein (about 52kDa), belongs to the intermediate filament family and Desmin is class III intermediate filaments found in muscle cells. Homopolymers of Desmin form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane.Mutations in Desmin are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies.Desmin is also expressed in smooth muscle cells of both airways and alveolar ducts and Desmin is a load-bearing protein that stiffens the airways and consequently the lung and modulates airway contractile response.
Synaptophysin (p38) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells.Synaptophysin contains four transmembrane domains that form a hexameric channel or gap junction-like pore. Synaptophysin binds to the SNARE protein synaptobrevin/VAMP, which prevents the inclusion of synaptobrevin in the synaptic vesicle fusion complex and creates a pool of synaptobrevin for exocytosis when synapse activity increases. Synaptophysin is also responsible for targeting synaptobrevin 2/VAMP2 to synaptic vesicles, a critical component of the fusion complex.
Cyclin D1, is cell cycle regulator and it is over expressed in a wide variety of human neoplasms. Cyclin D1 forms a complex with regulatory subunit of CDK4 or CDK6 kinases and it is required for cell cycle G1/S transition. The expression is maximal in G1 and minimal in S phase of cell cycle. Cyclin D1 expression is located mainly to the proliferative zone of normal epithelial tissues. Localization of the cyclin D1 is mainly nuclear. Cyclin D is useful for lymphoma diagnostic, especially diagnosis of mantle cell lymphoma.
Granzyme B (GZMB), is the cell death-inducing serine protease, which expressed in the cytotoxic T lymphocytes and natural killer (NK) cells. Granzyme B is crucial for the rapid induction of target cell apoptosis and it has essential role in immunosurveillance. Granzyme B enters in the target cells with perforin, and results in the activation of apoptosis through caspase-dependent and -independent pathways. Granzyme B is the useful marker especially in NK/T-cell lymphomas.
Glutamine synthetase is enzyme which catalyzes the synthesis of glutamine from glutamate and ammonia in the liver tissue. In normal liver glutamine sythetase expressed in pericentral hepatocytes. Glutamine synthetase can be useful marker in hepatocellular carcinoma diagnostic with panel of other hepatocellular carcinoma markers.
The protein encoded by this gene is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon.Tissue specificity: Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP1 and GLP2 are also secreted in selected neurons in the brain.
Glial Fibrillary Acidic Protein (GFAP) is the intermediate filament protein which is highly specific to astrocytes in the central nervous system (CNS). GFAP is also expressed some cells in peripheral nervous system eg. in Schwann cells and satellite cells. GFAP is useful especially for differential diagnosis of astrocytoma from non-glial neoplasm. Schwannoma and neurofibroma frequently express GFAP.
CD34 is a transmembrane glycoprotein with a molecular mass of approximately 110 kD that is selectively expressed on human hematopoietic progenitor cells, endothelial cells and some fibroblasts. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells. CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.
Beta-Catenin is a member of catenin family together with alpha and gamma catenin. It mediates cell-cell adhesion with cadherins and it is key regulatory protein in signaling through the WNT pathway. Beta catenin has a role in cellular proliferation, differentiation and development. Mutations in beta catenin gene (CTNNB1) leads accumulation of the beta catenin protein in cytoplasm and nucleus in different type of tumors eg. endometrial carcinoma and desmoid tumors. This antibody is useful in differentiation diagnostic of tumors.
10 mM Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.6
Reconstitution:
Rehydrate with 2.0 ml of deionized water and centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store lyophilized material at 2-8 °C. For long term storage after reconstitution, prepare small aliquots and store at -20 °C . For storage at 2-8 °C, add a preservative to prevent growth of bacteria.
Specificity:
Based on IEP, this antibody reacts with: · F(ab)2 Fragment of Rat IgG
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin Rat serum proteins · Fc Fragment of Rat IgG
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
10 mM Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.6
Reconstitution:
Rehydrate with 2.0 ml of deionized water and centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store lyophilized material at 2-8 °C. For long term storage after reconstitution, prepare small aliquots and store at -20 °C . For storage at 2-8 °C, add a preservative to prevent growth of bacteria.
Specificity:
Based on IEP, this antibody reacts with: · F(ab)2 fragment of Rabbit IgG
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin Rabbit serum proteins · Fc fragment of Rabbit IgG
Country Of Origin:
Normal Goat Serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
10 mM Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.6
Reconstitution:
Rehydrate with 2.0 ml of deionized water and centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
2-8 °C
Specificity:
Based on IEP, this antibody reacts with: · F(ab)2 fragment of Mouse IgG
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin Mouse serum proteins · Fc fragment of Mouse IgG
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
The antibody reacts with mouse EGF in ELISA (10 ng detectable) and in spot blots (1 ng detectable). In immunohistochemistry the antibody reacts with mouse salivary glands. No crossreaction with rat EGF.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
E5
Concentration:
5 ug/ml
Storage buffer:
Culture medium with 0.01M Sodium Azide
Storage:
2-8°C
References 1:
Beerstecher HJ et al. (1988) J Histochem Cytohistochem 36, 1153-1160
The antibody reacts with free kappa chains, but not with kappa chains attached to heavy chains. No reaction is observed with Ig kappa on mem¬branes of lymphocytes. No crossreactivity with lambda chains. In Ouchterlony immunodiffusion no reaction with Bence Jones kappa is observed.
The antibody will stain lambda chain-containing human cells. In ELISA the antibody will react with free lambda chains and also with lambda chains attached to heavy chains. Membrane bound Ig containing lambda light chains will also be recognized as well as cytoplasmic Ig from bone marrow cells containing lambda chains. All cells reacting with conventional anti-lambda will also react with antibody. No crossreactivity with kappa chains detected.
The antibody only stains human and various animal blood vessels with exception of arteries. It does not stain rat, mouse and chicken endothelium. The antibody is useful for immunohistochemistry on acetone fixed frozen sections. Not useful on cellsuspensions and Western blotting.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
PAL-E
Concentration:
15 ug/ml
Storage buffer:
Culture medium with 0.01M Sodium Azide
Storage:
2-8°C
References 1:
Jones, R.R., et al., J. Clin. Path. 39, 742-749 (1986).
References 2:
Holden, C.A., et al., J. Immun. Meth. 91, 45-52 (1986).
References 3:
Schlingemann, R.O., et al., Laboratory Investigation 52, 71 (1987).
Antibody PAL-M1 stains the majority of primary melanomas and the large majority of metastases and is directed against the transferrin receptor (CD71). Local staining of dysplastic nevocellular nevi may be observed. Common nevocellular nevi rarely stain.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
PAL-M1
Concentration:
15 ug/ml
Storage buffer:
Culture medium with 0.01M Sodium Azide
Storage:
2-8°C
References 1:
Ruiter DJ et al., (1985) J Invest Dermatol 85, 4-8
References 2:
Muijen G van et al. (1990) J Invest Dermatol 95, 65-69
The antibody stains over 95% of primary renal cell carcinomas and 60% of metastases of renal cell carcinoma and glomerular visceral epithelium and proximal tubules in normal kidney (cytoplasmic). It does not stain epithelial cells of non-renal malignancies.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
RC38
Concentration:
15 ug/ml
Storage buffer:
Culture medium with 0.01M Sodium Azide
Storage:
2-8°C
References 1:
Oosterwijk E et al. (1986). Am J Pathol 123, 301-309
The antibody stains 80% of non-mucinous primary and metastatic ovarian cancers. This monoclonal antibody is used for the identification of primary and metastatic non-mucinous ovarian carcinoma and ovarian carcinoma cells in peritoneal fluids. It rarely stains nongynaecological malignancies.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
OV632
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.01M Sodium Azide
Storage:
2-8°C
References 1:
Fleuren GJ et al. (1987) Virchows Archiv A, 410, 481-486
References 2:
Boerman OC et al. (1991) Int J Gynecol 10, 15-23
References 3:
Delahye H et al., (1991) J Pathol 165 137-143
References 4:
Boerman OC et al. (1991) Anticancer Res 10, 1289-1296
This antibody stains a minority of primary melanomas and half of the metastatic lesions tested. It rarely stains dysplastic naevi or common cellular naevi using standard immunohistochemical conditions. The antibody recognizes two protein bands in immunoblotting with a molecular weight of 95-100 kD.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
PAL-M2
Concentration:
10 ug/ml
Storage buffer:
Culture medium with 0.01M Sodium Azide
Storage:
2-8°C
References 1:
Ruiter DJ et al., (1985) J Invest Dermatol 85, 4-8
The antibody reacts with mouse EGF in ELISA and in spot blots. In immunohisto¬chemistry the antibody reacts with formalin fixed and paraffin embedded mouse salivary glands. It also reacts with human Brunner's glands (presumably with urogastr¬on).
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
F5
Concentration:
10 ug/ml
Storage buffer:
Culture medium with 0.01M Sodium Azide
Storage:
2-8°C
References 1:
Beerstecher HJ et al. (1988) J Histochem Cytohistochem 36, 1153-1160
Goat anti-Dog serum proteins is a secondary antibody which binds to dog serum proteins, in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Reconstituted material can be stoored in 4°Cup to 7 days.
Host Animal:
Goat
Species Reactivity:
Dog serum proteins
Immunogen:
Pooled whole dog serum and partly purified serum fractions
In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted in 2 mm diameter peripheral wells. Different bleedings of the immunized animals are pooled to obtain a broad spectrum balanced against the varying concentrations of the individual serum protein components.
Purity:
Serum
Reconstitution:
For reconstitution add 1 ml of sterile destilled water
This antibody is highly reactive with desmin. On immunoblots only the 52 kD desmin band is stained. Immunogen derived from human Leiomyoma. No cross reactivity with GFAP, Keratin, Vimentin or Neurofilament detected.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
D33
Concentration:
20 ug/ml
Storage buffer:
Culture medium with 0.01M Sodium Azide
Storage:
2-8°C
References 1:
Muijen GNP van et al. (1987) Lab Invest 57, 359-369
References 2:
Seidal T et al. (1987) Appl Pathol 5, 201-219
References 3:
Ramaekers F et al. (1983) Histochemical J 15, 691-713
References 4:
Broek JCM van et al. (2000) Appl Immunohis & Mol Morph 8, 316-321
The antibody stains non-cornifying squamous epithelium and transitional epithelium of the bladder, cells in certain ciliated pseudo-stratified epithelia and ductal epithelia of various exocrine glands. In immunoblotting experiments the antibody was shown to recognize only cytokeratin no.4 (59 kDa).
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
6B10
Concentration:
15 ug/ml
Storage buffer:
Culture medium with 0.01M Sodium Azide
Storage:
2-8°C
References 1:
Muijen GNP et al. (1986) Exp Cell Res 162, 97-113
References 2:
Muijen GNP et al. (1987) Exp Cell Res 171, 331-345
The antibody does stain especially simple epithelia. Only frozen sections can be used in immunoperoxidase and immunofluorescence tests. In immunoblotting experiments the antibody was shown to recognize only keratin no.18 (45 kDa).
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
M9
Concentration:
15 ug/ml
Storage buffer:
Culture medium with 0.01M Sodium Azide
Storage:
2-8°C
References 1:
Muijen GNP van et al. (1987) Exp Cell Res 171, 331-345
The antibody does stain all cells that contain vimentin. Only frozen section can be used. The antibody only stains the 57kDa vimentin band in immunoblots performed on a lysate of human lymphocytes.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
V9
Concentration:
25 ug/ml
Storage buffer:
Culture medium with 0.01M Sodium Azide
Storage:
2-8°C
References 1:
Muijen GNP van (1984) Am J Pathol 116, 363-369
References 2:
Debus E et al. (1984) Eur J Cell Biol 34, 137-143
References 3:
Muijen G van (1986) Exp Cell Res 162; 97-113
References 4:
Corver WE et al. (1994) Cytometry 15, 117-128
References 5:
Broek JCM van den et al. (2000) Appl Immunohis & Mol Morphology 8, 316-321
The antibody does stain neurons in sections of brain and other tissues. On an immunoblot on a crude human brain extract the antibody will only stain the 70 kDa band of neurofilament (ref. 1)
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
2F11
Concentration:
15 ug/ml
Storage buffer:
Culture medium with 0.01M Sodium Azide
Storage:
2-8°C
References 1:
Muijen GNP van et al. (1984) Am J Pathol. 116, 363-369
References 2:
Klück P et al. (1984) Lancet (March 24th), 652-653
References 3:
Muijen GNP van et al. (1985) Hum Pathol 16, 590-595
References 4:
Banks-Schlegel SP et al. (1985) Cancer Res 406, 339
The antibody does not stain non-cornified squamous epithelia, except cornea and transitional epithelial regions, with the exception of basal cell layers of some stratified epithelia (ref. 1). In immunoblotting experiments the antibody was shown to recognize only cytokeratin no. 13 (54 kDa).
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
1C7
Concentration:
15 ug/ml
Storage buffer:
Culture medium with 0.01M Sodium Azide
Storage:
2-8°C
References 1:
Muijen GNP et al. (1986) Exp Cell Res 162, 97-113
References 2:
Muijen GNP et al. (1987) Exp Cell Res 171, 331-345
The antibody does stain all types of keratin containing (that is epithelial) cells in frozen sections of various tissues, with the exception of myo-epithelial cells and the podocytes of the glomeruli.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
80
Concentration:
15 ug/ml
Storage buffer:
Culture medium with 0.01M Sodium Azide
Storage:
2-8°C
References 1:
Muijen GNP van (1984) Am J Pathol 114, 9
References 2:
Muijen GNP van (1984) Am J. Pathol 116, 363
References 3:
Muijen GNP van (1985) Hum Pathol 16, 590
References 4:
Corver WE et al. (2000) Cytometry 41, 73-80
References 5:
Broek, JCM van et al. (2000) Appl. Immunohis. & Mol. Morph. 8, 316-321
The antibody does not stain non-cornifying squamous epithelium and transitional epithelium of the bladder. In immunoblotting experiments the antibody recognizes only keratin no. 13 (54 kDa).
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
2D7
Concentration:
15 ug/ml
Storage buffer:
Culture medium with 0.01M Sodium Azide
Storage:
2-8°C
References 1:
Muijen GNP et al. (1986) Exp Cell Res 162, 97-113
References 2:
Niekerk CC van et al. (1991) J Pathol 165, 145
References 3:
Ramaekers F (1990) Am J Pathol 136, 641
References 4:
Niekerk CC van et al. (1989) Int J Cancer 43, 1065
The antibody stains cells containing GFAP. The antibody only stains the 56kD GFAP band in immunoblots performed on crude brain extract. It reacts also with mouse GFAP
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
6F2
Concentration:
25 ug/ml
Storage buffer:
Culture medium with 0.01M Sodium Azide
Storage:
2-8°C
References 1:
Muijen GNP van et al. (1987) Lab Invest 57, 359-369
SPY (SPINDLY) is O-linked N-acetylglucosamine transferase (OGT) involved in various processes such as gibberellin (GA) signaling pathway and circadian clock. It catalyze the addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Acts as a repressor of GA signaling pathway to inhibit hypocotyl elongation. Alternative name: Probable UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SPINDLY.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Recombinant SPY protein
Expected Species:
Arabidopsis thaliana, Brachypodium distachyon, Glycine max, Helianthus annus, Hordeum vulgare, Oryza sativa, Physcomitrella patens, Solanum lycopersicum, Sorghum bicolor, Zea mays, Vitis viniferaSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SPY protein sequence, UniProt: Q96301, TAIR: At3g11540
This antibody so far has been confirmed to detect recombinant SPY protein, GFP-tagged. Optimization of protein extraction and detection using most sensitive reagents may result in recognition of endogenous SPY.
Application Details:
1 : 500 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water to each tube
Molecular Weight:
101,4 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
ATG8 (Autophagy-related protein) involved in degradation and recycling of intracellular components in a process of autophagy.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Recombinant ATG8 A-I of Arabidopsis thaliana
Immunogen:
Part of recombinant ATG8A from Arabidopsis thaliana, UniProt:Q8LEM4,TAIR:AT4G21980
Protein pelota homolog is involved in the mechanism for releasing non-functional ribosomes and degrading damaged mRNA. This protein belongs to the eukaryotic release factor 1 (ERF1) family, and the Pelota subfamily.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
AKIN 3 (SNF1-related protein kinase regulatory subunit beta-3) is a regulatory subunit of the probable trimeric SNF1-related protein kinase (SnRK) complex, which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brasica rapa, Capsella rubella, Ricinus communis, Vitis vinifera Species of your interest not listed? Contact us
AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
FIE (fertilization-independent endosperm) belongs to polycomb group (PcG) protein which act by forming multiprotein complexes, required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Recombinant FIE protein
Expected Species:
Arabidopsis thaliana, Solanum lycopersicum, Triticum aestivumSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana FIE protein sequence, UniProt: Q9LT47, TAIR: At3g20740
This antibody so far has been confirmed to detect recombinant FIE protein, GFP-tagged. Optimization of protein extraction and detection using most sensitive reagents may result in recognition of endogenous FIE.
Application Details:
1 : 1000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
41 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
LSD1 (Lesion simulating disease 1) monitors a superoxide-dependent signal and negatively regulates a plant cell death pathway. contains zinc-finger motifs. LSD1 negatively regulates a basal defense pathway that can act upstream or independently of both NIM1/NPR1 function and SA accumulation. Alternative names: CHILLING SENSITIVE 4, CHS4.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4 C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Chicken
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica olereacea, Pisum sativum Species of your interest not listed? Contact us
PPD2 (protein PEABOD 2) belongs to the TIFY/JAZ family and is involved in lamina development, regulating leaf size and its curvature. Alternative name: Protein TIFY4B
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
ATG5 (Autophagy-related protein 5) the protein forms a conjugate with ATG12 with an essential role in plant nutrient recycling. It is involved in complete proteolysis of chloroplast stroma proteins in senescent leaves and degradation of damaged peroxisomes.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Recombinant ATG5 of Arabidopsis thaliana
Immunogen:
Recombinant ATG5 of Arabidopsis thaliana, UniProt:Q9FFI2, TAIR:AT5G17290
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Young et al. (2019). A facile forward-genetic screen for Arabidopsis autophagy mutants reveals twenty-one loss-of-function mutations disrupting six ATG genes. Autophagy. 2019 Jun;15(6):941-959. doi: 10.1080/15548627.2019.1569915.
Special application note:
This antibody is so far recognizing recombinant ATG5 from Arabidopsis thaliana and reactivity on endogenous protein needs to be confrimed. It does not react with 6xHis-ATG7.This product can be sold containing ProClin if requested.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified donkey IgG.
Special application note:
Antibody purity is > 90% based on SDS-PAGE Small amounts of intact IgG may be present.Affinity purified using solid phase Mouse IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on mouse IgG • light chains on all mouse immunoglobulins .Based on IEP, no reactivity is observed to: • non-immunoglobulin mouse serum immunoglobulins .
Grx5 | Monothiol glutaredoxin-S5 - thioredoxin proteins function in electron carrier activity and are involved in cell redox homeostasis. Synonymes: ROXY 12 (GrxS5).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Glycne soja, Medicago truncatula, Morus notabilis, Theobroma cacao Species of your interest not listed? Contact us
Immunogen:
His-tagged, full length recombinant Grx5 of Arabidopsis thaliana, UniProt: O23420, TAIR:AT4G15690. There is high homology of this sequence with GrxS3 UniProt: O23421, TAIR: AT4G15700, GrxS4 UniProt: O23419, TAIR:AT4G15680 , Grx7 UniProt:Q6NLU2, TAIR:AT4G15670, GRx8 UniProt: O23417, TAIR:AT4G15660
This antibody works also on recombinant Grx5 from Arabidopsis thaliana.
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
11 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Nath et al. (2016). A Nitrogen-Fixing Subunit Essential for Accumulating 4Fe-4S-Containing Photosystem I Core Proteins. Plant Physiol. 2016 Dec;172(4):2459-2470. Epub 2016 Oct 26.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifugate to remove any particulates, Prepare fresh working dilutions daily
Not reactive in:
Non-immunoglobulin hamster serum proteins
Special application note:
Antibody purity is >95% based on HPLC.Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.1% (v/v) Kathon CG is added as a preservative.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily
Not reactive in:
Non-immunoglobulin bovine serum proteins
Special application note:
Antibody purity is > 95% based on SDS-PAGE.Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.1% (v/v) ProClin 150 is added as a preservative.
Oxidative derivate of guanosine is called 8-Hydroxyguanosine (8OHdG) and is used as a popular biomarker of oxidative stress.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Recognizes markers of oxidative damage to DNA (8-hydroxy-2’-deoxyguanosine, 8-hydroxyguanine and 8-hydroxyguanosine)
Immunogen:
8-hydroxy-guanosine-BSA and – casein conjugates
Applications:
ELISA (ELISA), Immunoaffinity chromatography (IAP), Immunohistochemistry on frozen tissue and paraffin-embedded (IHC-Fr-P)
Protocol for immunostaining using this antibody can be found here.
Application Details:
The optimal working dilution should be determined by the investigator
Conjugation:
IgG2A
Isotype:
IgG2A
Purity:
Total IgG fraction. Protein G purified.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Poborilova et al. (2015). DNA hypomethylation concomitant with the overproduction of ROS induced by naphthoquinone juglone on tobacco BY-2 suspension cells. Environmental and Experimental Botany, Volume 113, May 2015, Pages 28–39.Haigh and Drew (2015). Cavitation during the protein misfolding cyclic amplification (PMCA) method - The trigger for de novo prion generation? Biochem Biophys Res Commun. 2015 Apr 17. pii: S0006-291X(15)00726-3. doi: 10.1016/j.bbrc.2015.04.048.
Special application note:
Protein G purified IgG2B in PBS, pH 7,4 with 0,09 % sodium azide and 50 % glycerol at concentration 0,65 mg/ml
HDEL (his-asp-glu-leu) is a C-terminal tertapeptide found in yeast and plants which allows sorting of resident soluble proteins in the lumen of the endoplasmic reticulum.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20 °C for 1 year; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Antibody in concentration 1 g/ml was sufficient for detection of HDEL-containing proteins in 10 g of yeast cell lysate by colorimetric western blot. Clone 2E7.For western blot results, immunofluorescence and immunogold images please referr to Napier et al. 1992.
Application Details:
1, 50-1 : 500 (IF), 1 : 100-1, 1000 (WB)
Conjugation:
IgG2B
Isotype:
IgG2B
Purity:
Total IgG. Protein G purified in PBS pH 7.4.
Molecular Weight:
78 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Ming-fang et al. (2021) Improved quantification of immune-gold labeling and its use to compare the distribution of cellular factors among sub-chloroplast compartments,Micron,2021,103060,ISSN 0968-4328,https://doi.org/10.1016/j.micron.2021.103060.Luo et al. (2006). GRP78/BiP is required for cell proliferation and protecting the inner cell mass from apoptosis during early mouse embryonic development. Mol Cell Biol. 26(15): 5688-5697.Napier et al. (1992). Immunological evidence that plants use both HDEL and KDEL for targeting proteins to the endoplasmic reticulum. J Cell Sci. 102: 261-271.
Special application note:
Protein G purified IgG2B in PBS, pH 7,4 with 0,09 % sodium azide and 50 % glycerol at concentration 1 mg/ml
Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common posttranscriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3).
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Bovine, avian
Expected Species:
Higher plants
Immunogen:
acetylated KLH
Applications:
ELISA (ELISA), Immunocytochmistry/Immunofluorescence (ICC/IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB)
1 g of this antibody is sufficient to detect acetylated chicken erythrocyte histones (sodium butyrate-treated) using 20 g total protein and ECL detection system
Application Details:
1 : 100 (IHC), 1 : 1000 (WB)
Conjugation:
IgG1
Isotype:
IgG1
Purity:
Total IgG fraction. Protein G purified.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Vigushin & Coombes (2004). Targeted histone deacetylase inhibition for cancer therapy. Curr. Cancer Drug Targets 4: 205-218.
Special application note:
Protein G purified IgG2B in PBS, pH 7,4 with 0,09 % sodium azide and 50 % glycerol at concentration 1 mg/mlantibody detects Proteins containing acetylated lysine residues in ELISA and WBs, Does not detect non-acetylated lysine residues
Nitrotyrosine is a marker of NO-dependent oxidative stress. It is a product of tyrosine nitration mediated by reactive nitrogen species. Protein tyrosine nitration results in a post-translational modification, component of nitric oxide signaling.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
The antibody recognizes 3-nitrotyrosine moieties. No detectable crossreactivity with non-nitrated tyrosine. Not species specific.0.7μg/ml was sufficient for detection of 5 μg SIN-1 treated BSA by Western Blot.Antibody works paraffin-embedded sections.
Application Details:
1: 100 (IHC), 1: 1400 (WB), The exact and optimal working dilution should be determined by the investigator
Conjugation:
IgG2A
Isotype:
IgG2A
Purity:
Total IgG. Protein G purified, in PBS. Contains 50 % glycerol and 0.09% sodium azide.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Gow et al. (2004).Biological significance of nitric oxide-mediated protein modifications. Am J Physiol Lung Cell Mol Physiol. 287(2): L262-8.Antibody used in immunohistochemistry:Pfister et al. (2002). Inducible nitric oxide synthase and nitrotyrosine in listeric encephalitis: a cross-species study in ruminants. Vet Pathol. 39: 190-199.Girault et al. (2001).Immunodetection of 3-nitrotyrosine in the liver of zymosan-treated rats with a new monoclonal antibody: comparison to analysis by HPLC. Free Radical Biology and Medicine, 31 (11): 1375-1387.
Special application note:
1 mg/ml of Protein G purified IgG2A in PBS pH 7,4, 0,09 % sodium azide, 50 % glycerol
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Donkey
Immunogen:
Purified Rabbit IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20°C.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rabbit IgG · light chains on all rabbit immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rabbit serum proteins · mouse IgG or serum proteins
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Trademark:
DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Donkey
Immunogen:
Purified Rabbit IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20°C.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rabbit IgG · light chains on all rabbit immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rabbit serum proteins · mouse IgG or serum proteins
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Trademark:
DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Donkey
Immunogen:
Purified Rabbit IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20°C.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rabbit IgG · light chains on all rabbit immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rabbit serum proteins · mouse IgG or serum proteins
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Trademark:
DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Donkey
Immunogen:
Purified Rabbit IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20°C.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rabbit IgG · light chains on all rabbit immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rabbit serum proteins · mouse IgG or serum proteins
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Trademark:
DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Sheep anti-Gamma-synuclein Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
Gamma synuclein belongs to the synuclein family which are believed to be involve in the pathogenesis of neurodegenerative diseases. High levels of gamma synuclein have been identified in andvanced breast carcinomas suggesting a correlation between gamma synuclein overexpression and breast tumor development. Gama synuclein plays a role in neurofilament network integrity. May be involved in modulating axonal architecture during development and in the adult. In vitro, increases the susceptibility of neurofilament-H to calcium-dependent proteases. May also function in modulating the keratin network in skin. Activates the MAPK and Elk-1 signal transduction pathway. SUBUNIT: May be a centrosome-associated protein. SUBCELLULAR LOCATION: Cytoplasm; perinuclear region. Centrosome. Spindle. Associated with centrosomes in several interphase cells. In mitotic cells, localized to the poles of the spindle. TISSUE SPECIFICITY: Highly expressed in brain, particularly in the substantia nigra. Also expressed in the corpus callosum, heart, skeletal muscle, ovary, testis, colon and spleen. Weak expression in pancreas, kidney and lung. PTM: Phosphorylated. Phosphorylation by GRK5 appears to occur on residues distinct from the residue phosphorylated by other kinases. DISEASE: Brain iron accumulation type 1 (NBIA1, also called Hallervorden-Spatz syndrome), a rare neuroaxonal dystrophy, is histologically characterized by axonal spheroids, iron deposition, Lewy body (LB)-like intraneuronal inclusions, glial inclusions and neurofibrillary tangles. SNCG is found in spheroids but not in inclusions.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (EKEEVAEEAQSGGD) as part of human gamma synuclein protein (114-127) conjugated to diphteria toxid has been used as the immunogen.
Applications:
IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
IHC, WB. A concentration of 2-5 µg/mL is recommended for both applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Persyn; Breast cancer-specific gene 1 protein; Synoretin; SR; SNCG
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Immunohistochemical/western blot anlysis indicate a high level of specificity for this antiserum for gamma synuclein. This antiserum is known to react with human and rat gamma synuclein.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Purification:
Affinity purified and dialysed against PBS. Contains 0.02% sodium azide.
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Host:
Donkey
Immunogen:
Purified Sheep IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on sheep IgG · light chains on all sheep immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin sheep serum proteins
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Host:
Donkey
Immunogen:
Purified Rat IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rat IgG · light chains on all rat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rat serum proteins · IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit or sheep
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Host:
Goat
Immunogen:
Purified Mouse IgG, whole molecule
Buffer:
30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum immunoglobulins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Host:
Donkey
Immunogen:
Purified Mouse IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum immunoglobulins
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Goat anti-mouse IgG (H&L) is a biotinylated secondary antibody which binds to mouse IgG (H&L). Min. cross-reactivity to human IgG/serum. Antibodies are affinity purified using solid phase mouse IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
Needs to be determined by end user for all immunoassay applications
Purity:
Immunogen affinity purified goat IgG.
Special application note:
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis, No reactivity is observed to non-immunoglobulin mouse serum proteins and human IgG or serum proteins based on immunoelectrophoresis
Rabbit anti-human IgM ( chain) is a secondary antibody which binds to human IgM (m chain) in immunological assays. Antibody is affinity purified using solid phase human IgG (H&L).
This antibody is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified rabbit IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is > 4.5 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on human IgM Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin human serum proteins light chains on all human immunoglobulins
Goat anti-human IgA (α chain), DyLight 488 Conjugated is a secondary antibody conjugated to DyLight 488, which binds to Human IgA (α chain) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Human IgADyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (α) chains on human IgA (α chain)No reactivity is observed to: non-immunoglobulin human serum proteins, light chains on all human immunoglobulins
Rabbit anti-llama IgG (H&L) is a secondary antibody conjugated to biotin, which binds to llama IgG in immunological assays. Antibody is affinity purified using solid phase llama IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 2000-1 : 20 000 (WB) 1 : 500-1 : 5 000 (IHC)
Purity:
Immunogen affinity purified rabbit IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on llama IgG, light chains on all llama immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin llama serum proteins.This antibody will react with VHH of llama IgG's.
Rabbit anti-llama IgG (H&L) is a secondary antibody which binds to llama IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase llama IgG (H&L).
This antibody is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified rabbit IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is > 4.5 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on llama IgG, light chains on all llama immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin llama serum proteins.This antibody will react with VHH of llama IgG's.
Rabbit anti-human IgG (H&L) is a secondary antibody conjugated to HRP, which binds to human IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase human IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified rabbit IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml. Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.1 % (v/v) Kathon CG as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on human IgG, light chains on all human immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin human serum proteins.
Rabbit anti-human IgG (H&L) is a secondary antibody conjugated to biotin, which binds to human IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase human IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 2000-1 : 20 000 (WB) 1 : 500-1 : 5 000 (IHC)
Purity:
Immunogen affinity purified rabbit IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on human IgG, light chains on all human immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin human serum proteins
Rabbit anti-human IgG (H&L) is a secondary antibody conjugated to ALP, which binds to human IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase human IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 500-1 : 2000 (IHC), 1 : 50 000-1 : 5 000 (WB)
Purity:
Immunogen affinity purified rabbit IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.50 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 30 mM triethanolamine, pH 7.2.5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on human IgG, light chains on all human immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin human serum proteins
Rabbit anti-cat IgG (H&L) is a secondary antibody which binds to cat IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase cat IgG (H&L).
This antibody is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified rabbit IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is > 4.5 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on cat IgG light chains on all cat immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin cat serum proteins
Goat anti-rabbit IgG (H&L) is a secondary antibody conjugated to ALP, which binds to rabbit IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rabbit IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard. Shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.50 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 30 mM triethanolamine, pH 7.2.5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with:heavy chains on rabbit IgG, light chains on all rabbit immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rabbit serum proteins, serum proteins from human, mouse or rat.
Goat anti-biotin is a secondary antibody conjugated to ALP, which binds to biotin in immunological assays. Antibody is affinity purified using solid phase biotin.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard. Shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 500-1 : 2000 (IHC), 1 : 50 000-1 : 5 000 (WB)
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE, Antibody concentration is 1,50 mg/ml (E 1% at 280 nm = 13,0), Antibody is supplied in 30 mM triethanolamine, pH 7,2,5 mM magnesium chloride, 0,1 mM zinc chloride, 1 % (w/v) B, Protease/IgG free, Contains 0,05% (w/v) sodium azide as preservative of bacterial growth
Goat anti-mouse IgG (H&L) is a secondary antibody conjugated to FITC, which binds to mouse IgG in immunological assays. Antibody is affinity purified using solid phase mouse IgG (H&L). Fluorescein-5-isothiocyanate (FITC) has Amax = 494 nm; Emax = 518 nm
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on mouse IgG, light chains on all mouse immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin mouse serum proteins, serum proteins from bovine, horse, human, pig, or rabbit.
Goat anti-mouse IgG (H&L) is a secondary antibody conjugated to biotin, which binds to mouse IgG (H&L) in immunological assays. Antibody is affinity purified using solid phaseMouse IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 2000-1 : 20 000 (WB) 1 : 500-1 : 5 000 (IHC)
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on mouse IgG, light chains on all mouse immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin mouse serum proteins, serum proteins from bovine, horse, human, pig, or rabbit.
Goat anti-human kappa (k) chain is a secondary antibody which binds to human kappa chain in immunological assays. Antibody is affinity purified using solid phase human kappa chain.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is > 4.5 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: kappa (k) light chains on human immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin human serum proteins, heavy chains on human immunoglobulins, lambda light chains on human immunoglobulins.
Purified rabbit IgG, F(ab)'2 fragment contains Protein A purified rabbit IgG from normal serum and is excellent for use as blocking reagent in immunoassays.
Purified IgG, F(ab)'2 fragment in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2. Contains 0.05 % sodium azide.
Special application note:
Rabbit IgG, F(ab)'2 fragment is provided in 10 mM sodium phosphate, 0.15M sodium chloride, pH 7.2, 0.05 % sodium azide is added as preservative.Concentration is 4.5 mg/ml.Purity >95 % by SDS-PAGE.
Purified human IgG, F(ab)'2 fragment contains Protein A purified human IgG from normal serum and is excellent for use as blocking reagent in immunoassays.
Purified IgG, F(ab)'2 fragment in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2.
Special application note:
Human IgG, F(ab)'2 fragment is provided in 10 mM sodium phosphate, 0.15M sodium chloride, pH 7.2, 0.05 % sodium azide is added as preservative.Purity >95 % by SDS-PAGE.Donor serum was tested by approved methods and found negative for antibodies to HIV 1/2, HCV, HBsAg, HIV-1 RNA, HCV and HBc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Donkey
Immunogen:
Purified Rabbit IgG, whole molecule
Buffer:
PBS, 1% BSA & 0.1% proclin 150
Preservative:
0.1% (v/v) Kathon CG
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rabbit IgG · light chains on all rabbit immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rabbit serum proteins · mouse IgG or serum proteins
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
The antibody reacts with a protein determinant of the extra cellular domain of the human EGFR, and does not cross react with EGFR from murine cells. The antibody shows binding competition with EGF. The antibody reacts in immunoprecipitation with the functional EFR protein-tyrosine kinase complex.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
2E9
Concentration:
100 ug/ml
Storage buffer:
PBS with 1% BSA & 0.1% sodium azide
Storage:
2-8°C
References 1:
Defize L.H.K, et al. The Journal of Cellbiology. 1989: 109:2495-2507
References 2:
Van der Burg M.E.L, et al. Eur J Cancer. 1993: Vol29A, No 14: 1951-1957
Normal Human Serum/Plasma was obtained from healthy donors of US origin.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
10 mM Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.6
Storage:
2-8 °C
Country Of Origin:
Normal Mouse Serum was obtained from healthy animals of Chinese origin.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Mouse IgM, µ chain
Buffer:
PBS, 1% BSA & 0.1% proclin 150
Preservative:
0.1% (v/v) Kathon CG
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.
Specificity:
Based on IEP, this antibody reacts with: · heavy (µ) chains on mouse IgM
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum proteins · light chains on all mouse immunoglobulins · human IgG or serum proteins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution:
Rehydrate with 11.0 ml of deionized water.(Product has been overfilled to ensure complete recovery.) Swirl gently and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates.
Storage:
Store lyophilized material at 2-8 °C. For long term storage after reconstitution, prepare small aliquots and store at -20 °C . For storage at 2-8 °C, add a preservative to prevent growth of bacteria.
Country Of Origin:
Normal Llama Serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Goat anti-mouse IgG (H&L), DyLight 488 Conjugated, min. cross-reactivity to bovine, goat, human, rabbit, rat IgG is a secondary antibody conjugated to DyLight 488, which binds to Mouse IgG (H&L) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase mouse IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG, light chains on all mouse immunoglobulinsNo reactivity is observed to: non-immunoglobulin mouse serum proteins, IgG from bovine, goat, human, rabbit or rat
Goat anti-mouse IgG (H&L) - F(ab)'2 fragment, DyLight 800 Conjugated, min. cross-reactivity to human IgG or serum proteins is a secondary antibody conjugated to DyLight 800, which binds to Mouse IgG (H&L) - F(ab)'2 fragment in immunological assays.DyLight 800 has Amax = 777 nm, Emax = 794 nm. Antibodies are purified using solid phase Mouse IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0,5 mg of antibody in 0,55 ml of sterile water add 0,55 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG, light chains on all mouse immunoglobulinsNo reactivity is observed to: non-immunoglobulin mouse serum proteins, human IgG or serum proteins
Goat anti-rabbit IgG (H&L) -F(ab)'2 fragment, DyLight 650 Conjugated, min. cross-reactivity to bovine, human, or mouse lgG or serum proteins is a secondary antibody conjugated to DyLight 650, which binds to Rabbit IgG (H&L) - F(ab)'2 fragment in immunological assays.DyLight 650 has Amax = 652 nm, Emax = 672 nm. Antibodies are purified using solid phase Rabbit IgGDyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0,5 mg of antibody in 0,55 ml of sterile water add 0,55 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG light chains on all rabbit immunoglobulinsNo reactivity is observed to: non-immunoglobulin rabbit serum proteins IgG from bovine, human or mouse serum proteins from bovine, human or mouse
Goat anti-human IgG Fc - DyLight 650 Conjugated, min. cross-reactivity to human IgA + IgM, bovine, horse, mouse, rabbit serum proteins is a secondary antibody conjugated to DyLight 650, which binds to human IgG Fc in immunological assays.DyLight 650 has Amax = 652 nm, Emax = 672 nm. Antibodies are are affinity purified using solid phase human IgG Fc.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on human IgGNo reactivity is observed to: non-immunoglobulin human serum proteins light chains on all human immunoglobulins human IgA + IgM serum proteins from bovine, horse, mouse or rabbit serum proteins
Goat anti-human IgA (α chain), DyLight 800 Conjugated is a secondary antibody conjugated to DyLight 800, which binds to human IgA (α chain) in immunological assays.DyLight 800 has Amax = 777 nm, Emax = 794 nm. Antibodies are purified using solid phase Human IgADyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (α) chains on human IgANo reactivity is observed to: non-immunoglobulin human serum proteins, light chains on all human immunoglobulins
Chicken anti-Goat IgG (H&L), DyLight 800 Conjugated, min. cross-reactivity to human, mouse or rabbit IgG and serum proteins is a secondary antibody conjugated to DyLight 800, which binds to Goat IgG (H&L) in immunological assays.DyLight 800 has Amax = 777 nm, Emax = 794 nm. Antibodies are purified using solid phase Goat IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0,5 mg of antibody in 0,55 ml of sterile water add 0,55 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on goat IgG, light chains on all goat immunoglobulinsNo reactivity is observed to: non-immunoglobulin goat serum proteins, IgG/serum from human, mouse or rabbit.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Mouse IgG + IgM.Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: • heavy chains on mouse IgG + IgM • light chains on all mouse immunoglobulins .Based on IEP, no reactivity is observed to: • non-immunoglobulin mouse serum proteins .
For reconstitution add 1,1 ml of sterile water,Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Hamster IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.1% (v/v) Kathon CG is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on hamster IgG • light chains on all hamster immunoglobulins .Based on IEP, no reactivity is observed to: • non-immunoglobulin hamster serum immunoglobulins • human serum proteins .
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Country Of Origin:
Normal Guinea Pig Serum was obtained from healthy animals of US origin.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified using solid phase Human Antithrombin III
Purity:
> 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Antithrombin III
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Specificity:
Human Antithrombin III
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Streptavidin has been conjugated to Horseradish Peroxidase (HRP) is used with biotinylated antibodies. Streptavidin binds to biotin and the conjugated HRP provides enzyme activity for detection using an appropriate substrate system. This particular product has been used primarily in sandwich ELISA applications to provide consistent measurement of biotinylated detection antibodies.
Concentration:
1.0 mg/ml (E 1% at 280 nm = 32.0)
Conjugate:
Horseradish Peroxidase
Form:
Lyophilized
Purification:
N/A
Host:
Bacterial
Immunogen:
N/A
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.1% (v/v) Kathon CG
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Country Of Origin:
US Origin
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
The Plus HRP Polymer anti-Mouse kit is designed for the qualitative detection of antigens in fixed paraffinembedded tissue sections, in frozen tissue sections, and in cytological samples. It was developed for use in combination with monoclonal primary antibodies and sera obtained from mice. The reagent can be used for examining tissues fixed in different solutions, e.g. formalin (neutrally buffered), B5, Bouin, ethanol, or HOPE.
The purpose of the immunohistochemical staining is to make tissue and cell antigens visible. The Plus HRP Polymer anti-Mouse kit is a highly sensitive detection kit intended for use in immunohistochemistry and immunocytochemistry. The enzyme polymer consists of several molecules of secondary antibodies covalently bound to several molecules of horse radish peroxidase (HRP). Visualisation occurs via an enzyme-substrate reaction in the presence of a colorising reagent which permits microscopical analysis. The test system is suitable for the detection of monoclonal primary antibodies and sera obtained from mice. In contrast to other detection techniques, which often use the streptavidin-biotin system the Plus HRP Polymer antiMouse kit avoids the problem of background staining caused by endogenous biotin in the tissue
Principle of method:
Paraffin-embedded tissue sections are first deparaffinised and rehydrated. Endogenous peroxidase activity in the tissue may cause non-specific staining. This enzyme activity can be blocked by incubation with 3% H2O2-solution (peroxide block). Background staining caused by unspecific binding of the primary antibody or the secondary antibody in the HRPpolymer is minimized by incubation with a protein blocking solution. This step can be omitted if the primary antibodies are diluted in an appropriate buffer. The next step is incubation with the specific primary antibody. After washing, the HRP-polymer is applied and incubated. Any excess of unbound HRP-polymer is thoroughly washed away after incubation. The addition of the chromogenic substrate starts the enzymatic reaction of the peroxidase which leads to colour precipitation where the primary antibody is bound. The colour can be observed with a light microscope. The chromogen used determines the colour. The chromogen AEC leads to the formation of a red-brown product of reaction at the place of the target antigen. The chromogen DAB forms a dark brown precipitate.
Reagents provided:
6 mL HRP-Polymer anti-Mouse (ready-to-use) Substrate systems recommended: Permanent AEC kit, AEC single solution, AEC substrate kit, DAB substrate kit, DAB High contrast kit. Materials required but not supplied: Positive und negative control tissue Xylene or suitable substitutes Ethanol, distilled H2O 3% H2O2 solution Reagents for enzyme digestion or heat pre-treatment Wash buffer PBS or TBS PAP Pen Primary antibody (user-defined) Primary antibody diluent Negative control reagent Chromogenic substrate Counter stain solution Mounting medium Cover slips
Storage and handling:
The solution should be stored at 2-8°C without further dilution. Please store the reagent in a dark place and do not freeze it. Under these conditions the solution is stable up to the expiry date. It should not be used after the expiry date. A positive and a negative control have to be carried out in parallel to the test material. If you observe unusual staining or other deviations from the expected results which could possibly be caused by the kit reagents, please contact our technical support .
Reagent preparation:
Reagent should be at room temperature when used. Deparaffinise and rehydrate paraffin-embedded tissue sections. Pre-treatment (optional) with HIER (Heat Induced Epitope Retrieval) or enzymatic digestion. Tissue sections have to be completely covered with the different reagents in order to avoid drying out.
Procedure:
1. Peroxide blocking (3 % H2O2 solution) 10 min. 2. Washing with wash buffer 1 x 2 min. 3. Blocking Solution (This step is optional.) 5 min. 4. Washing with wash buffer 1 x 2 min. 5. Primary antibody (optimally diluted) or negative control reagent 30-60 min. 6. Washing with wash buffer 3 x 5 min. 7. HRP-polymer anti-Mouse 30 min. 8. Washing with wash buffer 3 x 2 min. 9. AEC or DAB (Controlling the colour intensity via light microscope is recommended.) 5-15 min. 10. Stopping the reaction with distilled H2O when the desired colour intensity is attained 11. Counterstaining and blueing 12. Mounting: aqueous with AEC, permanent with DAB or Permanent AEC
Expected results:
During the reaction of the substrate with horse radish peroxidase in the presence of a chromogen, a coloured precipitate is formed at the location of the bound primary antibody. This reaction only takes place if the target antigen is existent in the tissue. The chromogen used determines the colour of the precipitate. The analysis is carried out using a light microscope.
Trouble shooting:
If you observe unusual staining or other deviations from the expected results which could possibly be caused by the reagents, please read these instructions carefully, contact our technical support . No staining on an actually positive control slide: 1. Reagents were not used in the proper order. 2. Chromogenic substrate solution was too old. 3. Bleaching because chromogen and mounting medium are incompatible. 4. The antigen/epitope in the tissue was insufficiently accessible to the primary antibody. Try a pre-treatment such as heat pre-treatment or enzyme digestion. If you used a pre-treatment it should be extended. 5. Primary antibody not from mouse but from a different species. 6. The antigen/epitope was not stable in the fixation and/or pre-treatment procedure used. Try another fixation or pre-treatment. Weak staining: 1. Inadequate fixation or overfixation. 2. Incomplete deparaffinisation. 3. The antigen/epitope in the tissue was insufficiently accessible to the primary antibody. If you used heat pre-treatment or enzyme digestion it should be extended. 4. Excessive incubation with Blocking Solution or insufficient washing after this step. 5. Too much wash buffer remains on the slides after washing, diluting the reagents applied in the next step. 6. Incubation times were too short or primary antibody concentration too low. 7. Chromogenic substrate solution was too old. Nonspecific background staining or overstaining: 1. Incomplete deparaffinisation. 2. Excessive tissue adhesive on slides. 3. Insufficient washing especially after the incubation with the enzyme polymer or the chromogenic substrate solution. These washings are critical. 4. Tissue was allowed to (partially) dry out with reagents on. 5. Unspecific binding of the primary antibody. Please use the Blocking Solution provided with this kit or dilute the primary antibody in appropriate diluents. 6. Incubation time of the primary antibody was too long or primary antibody concentration too high. 7. Incubation time of the chromogenic substrate solution was too long or reaction temperature too high (e.g. if temperature in the laboratory is high). 8. The substrate is metabolised by endogenous horse radish peroxidase in the tissue. Maybe the hydrogen peroxide solution used for blocking was inactivated
Quality Control:
We recommend carrying out a positive and a negative control with every staining run. The positive control permits the validation of appropriate processing of the sample. If the negative control has a positive result, this points to unspecific staining.
Performance characteristics:
Studies have been conducted to evaluate the performance of the kit reagents. The product has been found to be suitable for the intended use
Limitations of procedure:
Immunohistochemistry is a complex method in which histological as well as immunological detection methods are combined. Tissue processing and handling prior to immunostaining, for example variations in fixation and embedding or the inherent nature of the tissue can cause inconsistent results (Nadji and Morales, 1983). Endogenous peroxidase or pseudoperoxidase activity may cause non-specific staining. The enzyme activity is blocked by incubation with hydrogen peroxide solution. Tissues containing Hepatitis B Surface Antigen (HBsAg) may give false positive results with HRP (horse radish peroxidase) detection systems (Omata et al, 1980). Inadequate counterstaining and mounting can influence the interpretation of the results. The colour intensity of the reaction product can decrease with time, especially when exposed to light. Overexposure with the protein blocking solution can result in decreasing signal intensity. Sanbio guarantee that the product will meet all requirements described from its shipping date until its expiry date, as long as the product is correctly stored and utilized. No additional guarantees can be given. Under no circumstances shall Sanbio be liable for any damages arising out of the use of the reagent provided.
Precautions:
Use by qualified personnel only. Wear protective clothing to avoid eye, skin or mucous membrane contact with the reagents. In case of the reagent coming into contact with a sensitive area, wash the area with large amounts of water. Microbial contamination of the reagent must be avoided, since otherwise non-specific staining might appear. ProClin 950 is used for stabilisation. A Material safety data sheets (MSDS) is available upon request.
BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from a solution containing PBS pH 7.4, 3% trehalose, with 0.1% sodium azide.
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Recombinant human mature BDNF expressed in E.coli
Applications:
ELISA,ICC,WB
Clone number:
3C11
Antibody Isotype:
IgG
Application Details:
ELISA (50-100 ng/mL), WB (0.2 to 2 µg/mL), IF (2 to 20 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Detects human, mouse, rat, guinea pig BDNF. Expected to detect BDNF from other species due to sequence homology.
Storage:
Store lyophilized antibody at -20°C to -80°C protected from moisture. After reconstitution divide antibody into useful aliquots and keep aliquots at -20°C to -80°C for a higher stability. Working aliquots can be kept at 2-8°C for up to 1 month. Avoid repetitive freeze/thaw cycles.
Pepsin Solution is a ready-to-use solution developed for enzymatic epitope retrieval on formalin-fixed tissue sections on slides. This procedure (sometimes called PIER, Protease Induced Epitope Retrieval) is primarily used in immunohistochemical staining procedures.
Immunohistochemical staining procedures consist of sequential incubation steps with blocking solutions, antibodies and secondary reagents, enzymes and chromogenic substrates carried out on tissue sections. These tissue sections are mostly prepared out of formalin-fixed paraffin-embedded tissue blocks. Cellular structures are very effectively stabilised by formalin fixation which results in optimal morphological preservation of the sample. On the other hand the formalin fixation leads to strong cross-links between proteins. This means that epitopes of antigens are being masked and often are no longer accessible for primary antibodies. In order to enable primary antibodies to bind to antigens the epitopes have to be recovered. Enzymatic digestion with proteolytic enzymes (PIER) restores structures of the epitopes making them more accessible to specific antibodies. Heat induced epitope retrieval (HIER) in buffer solutions of different compositions and pH-values is another way of recovering epitopes. The primary antibody used determines the appropriate method.
Principle of method:
Pepsin Solution is a ready-to-use stabilised pepsin solution for enzymatic epitope retrieval.
Reagents provided:
60 ml Pepsin Solution (Ready-To-Use)
Storage and handling:
The solution should be stored at 2-8°C without furt her dilution. Do not freeze it. Under these conditions the solution is stable up to the expiry date indicated on the label. Do not use product after the expiry date. A positive and a negative control have to be carried out in parallel to the test material. If you observe unusual staining or other deviations from the expected results which could possibly be caused by this reagent, please contact our technical support
Reagent preparation:
The solution is ready-to-use.
Procedure:
Pepsin Solution is suitable for enzymatic epitope retrieval carried out after the dewaxing and rehydration of the tissue sections. 1. Cover deparaffinised and rehydrated tissue sections with ready-to-use Pepsin Solution. 2. Incubate for 10 - 15 minutes at 37°C or 20 30 minutes at room temperature. The optimal incubation time needs to be elaborated by the operator. It was shown that in individual cases a stronger signal can be obtained when the incubation time is elongated up to 120 minutes (e. g. for detection of Collagen IV with different antibodies). 3. Rinse carefully (3 x) with wash buffer. 4. Proceed with immunohistological staining as usual.
Expected results:
During the reaction of the substrate with horse radish peroxidase or alkaline phosphatase in the presence of a chromogen, a coloured precipitate is formed at the location of the bound primary antibody. This reaction only takes place if the target antigen is existent in the tissue. The chromogen used determines the colour of the precipitate. The analysis is carried out using a light microscope.
Trouble shooting:
If you observe unusual staining or other deviations from the expected results please read these instructions carefully, contact our technical support. Also refer to the instructions of the detection systems for guidance on general troubleshooting.
Quality Control:
We recommend carrying out a positive and a negative control with every staining run. The positive control permits the validation of appropriate processing of the sample. If the negative control has a positive result, this points to unspecific staining. Please refer to the instructions of the detection system for guidance on general quality control procedures.
Performance characteristics:
Studies have been conducted to evaluate the performance of the kit reagents. The product has been found to be suitable for the intended use
Limitations of procedure:
Immunohistochemistry is a complex technique involving both histological and immunological detection methods. It requires a highly trained histotechnologist. Tissue processing and handling prior to immunostaining, for example variations in fixation and embedding or the inherent nature of the tissue can cause inconsistent results (Nadji and Morales, 1983). Inadequate counterstaining and mounting can influence the interpretation of the results. Sanbio guarantees that the product will meet all requirements described from its shipping date until its expiry date, as long as the product is correctly stored and utilized. No additional guarantees can be given. Under no circumstances shall Sanbio be liable for any damages arising out of the use of the reagent provided.
Precautions:
Use by qualified personnel only. Wear protective clothing to avoid contact of reagent and specimen with eye, skin or mucous membranes. In case of reagent or specimen coming into contact with a sensitive area, wash area with large amounts of water. A material safety data sheet (MSDS) is available upon request.
BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from a solution containing PBS pH 7.4, 0.1% trehalose, with 0.1% sodium azide.
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Recombinant human mature BDNF expressed in E.coli
Applications:
ELISA,WB
Clone number:
4C8
Antibody Isotype:
IgG
Application Details:
<b>ELISA:</b> 50-100 ng/mL <br><br><b>Western Blotting (denaturing and reducing):</b> 0.2 to 2 µg/mL. M-1744-100 antibody detects 14 kDa mature BDNF monomer in human serum human and acid treated human and rodent whole tissue homogenates. In simple Tris-homogenate human brain lysates, M-1744-100 detects mature BDNF at 14kDa as well as an uncharacterized band at ~50kDa. No proBDNF band is detected in our samples, although M-1744-100 will detect proBDNF in purified form on blots. Additionally, M-1744-100 detects an additional band 18 kDa BDNF isoform human serum. For cell lysates, some caution is advised as in our hands M-1744-100 fails to detect mature BDNF on SH-SY5Y or C6 cell lysates but does detect mature BDNF in neuronal cell culture lysates. The reason for these differences has not been characterized. For detection of mature BDNF in cell lysates such as SH-SY5Y we recommend affinity-purified rabbit polyclonal antibody to rhBDNF (R-1707-100), or rabbit polyclonal antibody to BDNF peptide 1-10 (R-083-100, whole serum; R-066-500, IgG).<br><br><b>Immunofluorescence:</b> 1 to 4 µg/mL, 4% formaldehyde fixation and permeabilization. Staining can be weak until optimized.<br><br>M-1744-100 is not recommended for Flow Cytometry at this time.<br><br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Le Blanc J et al. (2020) Platelets Selectively Regulate the Release of BDNF, But Not That of Its Precursor Protein, proBDNF. Front Immunol. 11:575607 Application: Human, WB.
Specificity:
Detects human, mouse, rat, guinea pig BDNF. Expected to detect BDNF from other species due to sequence homology.
Storage:
Store lyophilized antibody at -20°C to -80°C protected from moisture. After reconstitution divide antibody into useful aliquots and keep aliquots at -20°C to -80°C for a higher stability. Working aliquots can be kept at 2-8°C for up to 1 month. Avoid repetitive freeze/thaw cycles.
The Plus HRP Polymer Kit is designed for the qualitative detection of antigens in fixed paraffin-embedded tissue sections, in frozen tissue sections, and in cytological samples. It is developed for use in combination with mono- and polyclonal primary antibodies and sera obtained from mice and rabbits. The kit can be used for examining tissues fixed in different solutions, e.g. formalin (neutrally buffered), B5, Bouin, ethanol, or HOPE.
The purpose of the immunohistochemical staining is to make tissue and cell antigens visible. The Plus HRP Polymer Kit is a highly sensitive detection kit intended for use in immunohistochemistry and immunocytochemistry. The enzyme polymer in this kit consists of several molecules of secondary antibodies covalently bound to several molecules of horse radish peroxidase (HRP). Visualisation occurs via an enzyme-substrate reaction in the presence of a colorising reagent which permits microscopical analysis. The test system is suitable for the detection of mono- and polyclonal primary antibodies and sera obtained from mice and rabbits. In contrast to other detection techniques, which often use the streptavidin-biotin system the Plus HRP Polymer Kit avoids the problem of background staining caused by endogenous biotin in the tissue
Principle of method:
Paraffin-embedded tissue sections are first deparaffinised and rehydrated. Endogenous peroxidase activity in the tissue may cause non-specific staining. This enzyme activity can be blocked by incubation with 3% H2O2-solution (peroxide block). Background staining caused by unspecific binding of the primary antibody or the secondary antibody in the HRPpolymer is minimized by incubation with a protein blocking solution (Blocking Solution, provided with the kit). This step can be omitted if the primary antibodies are diluted in an appropriate buffer. The next step is incubation with the specific primary antibody. After washing, the enhancement reagent (PostBlock) is applied and incubated. A second washing is followed by the application of the HRP-polymer. Any excess of unbound HRP-polymer is thoroughly washed away after incubation. The addition of the chromogenic substrate starts the enzymatic reaction of the peroxidase which leads to colour precipitation where the primary antibody is bound. The colour can be observed with a light microscope. The chromogen used determines the colour. The chromogen AEC leads to the formation of a red-brown product of reaction at the place of the target antigen. The chromogen DAB forms a dark brown precipitate.
Reagents provided:
6 ml Blocking Solution Reagent 1 (ready-to-use) 6 ml PostBlock Reagent 2 (ready-to-use) 6 ml HRP-Polymer (Mouse/Rabbit) Reagent 3 (ready-to-use) Substrate systems recommended: Permanent AEC kit, AEC single solution, AEC substrate kit, DAB substrate kit, DAB High contrast kit. Materials required but not supplied: Positive und negative control tissue Xylene or suitable substitutes Ethanol, distilled H2O 3% H2O2 solution Reagents for enzyme digestion or heat pre-treatment Wash buffer PBS or TBS Pink PAP Pen Primary antibody (user-defined) Primary antibody diluent Negative control reagent Chromogenic substrate Counter stain solution Mounting medium Cover slips
Storage and handling:
The solutions should be stored at 2-8°C without further dilution. Please store the reagents in a dark place and do not freeze them. Under these conditions the solutions are stable up to the expiry date indicated on the label. They should not be used after the expiry date. A positive and a negative control have to be carried out in parallel to the test material. If you observe unusual staining or other deviations from the expected results which could possibly be caused by the kit reagents, please contact our technical support.
Reagent preparation:
Reagents should be at room temperature when used. ? Deparaffinise and rehydrate paraffin-embedded tissue sections. ? Pre-treatment (optional) with HIER (Heat Induced Epitope Retrieval) or enzymatic digestion. ? Tissue sections have to be completely covered with the different reagents in order to avoid drying out.
Procedure:
1. Peroxide blocking (3 % H2O2 solution) 10 min. 2. Washing with wash buffer 1 x 2 min. 3. Blocking Solution (protein block, Reagent 1) (This step is optional.) 5 min. 4. Washing with wash buffer 1 x 2 min. 5. Primary antibody (optimally diluted) or negative control reagent 30-60 min. 6. Washing with wash buffer 3 x 5 min. 7. PostBlock (Reagent 2, yellow) 20 min. 8. Washing with wash buffer 3 x 5 min. 9. HRP-polymer (Reagent 3, red) 30 min. 10. Washing with wash buffer 3 x 2 min. 11. AEC or DAB (Controlling the colour intensity via light microscope is recommended.) 5-15 min. 12. Stopping the reaction with distilled H2O when the desired colour intensity is attained 13. Counterstaining and blueing 14. Mounting: aqueous with AEC, permanent with DAB
Expected results:
During the reaction of the substrate with horse radish peroxidase in the presence of a chromogen, a coloured precipitate is formed at the location of the bound primary antibody. This reaction only takes place if the target antigen is existent in the tissue. The chromogen used determines the colour of the precipitate. The analysis is carried out using a light microscope.
Trouble shooting:
If you observe unusual staining or other deviations from the expected results which could possibly be caused by the reagents, please read these instructions carefully, contact our technical support . No staining on an actually positive control slide: 1. Reagents were not used in the proper order. 2. Chromogenic substrate solution was too old. 3. Bleaching because chromogen and mounting medium are incompatible. 4. The antigen/epitope in the tissue was insufficiently accessible to the primary antibody. Try a pre-treatment such as heat pretreatment or enzyme digestion. If you used a pre-treatment it should be extended. 5. Primary antibody not from mouse or rabbit, but from a different species. 6. The antigen/epitope was not stable in the fixation and/or pre-treatment procedure used. Try another fixation or pre-treatment. Weak staining: 1. Inadequate fixation or overfixation. 2. Incomplete deparaffinisation. 3. The antigen/epitope in the tissue was insufficiently accessible to the primary antibody. If you used heat pre-treatment or enzyme digestion it should be extended. 4. Excessive incubation with Blocking Solution or insufficient washing after this step. 5. Too much wash buffer remains on the slides after washing, diluting the reagents applied in the next step. 6. Incubation times were too short or primary antibody concentration too low. 7. Chromogenic substrate solution was too old. Nonspecific background staining or overstaining: 1. Incomplete deparaffinisation. 2. Excessive tissue adhesive on slides. 3. Insufficient washing especially after the incubation with the enzyme polymer or the chromogenic substrate solution. These washings are critical. 4. Tissue was allowed to (partially) dry out with reagents on. 5. Unspecific binding of the primary antibody. Please use the Blocking Solution provided with this kit or dilute the primary antibody in appropriate diluents. 6. Incubation time of the primary antibody was too long or primary antibody concentration too high. 7. Incubation time of the chromogenic substrate solution was too long or reaction temperature too high (e.g. if temperature in the laboratory is high). 8. The substrate is metabolised by endogenous horse radish peroxidase in the tissue. Maybe the hydrogen peroxide solution used for blocking was inactivated.
Quality Control:
We recommend carrying out a positive and a negative control with every staining run. The positive control permits the validation of appropriate processing of the sample. If the negative control has a positive result, this points to unspecific staining.
Performance characteristics:
Studies have been conducted to evaluate the performance of the kit reagents. The product has been found to be suitable for the intended use
Limitations of procedure:
Immunohistochemistry is a complex method in which histological as well as immunological detection methods are combined. Tissue processing and handling prior to immunostaining, for example variations in fixation and embedding or the inherent nature of the tissue can cause inconsistent results (Nadji and Morales, 1983). Endogenous peroxidase or pseudoperoxidase activity may cause non-specific staining. The enzyme activity is blocked by incubation with hydrogen peroxide solution. Tissues containing Hepatitis B Surface Antigen (HBsAg) may give false positive results with HRP (horse radish peroxidase) detection systems (Omata et al, 1980). Inadequate counterstaining and mounting can influence the interpretation of the results. The colour intensity of the reaction product can decrease with time, especially when exposed to light. Overexposure with the protein blocking solution (Blocking Solution) can result in decreasing signal intensity. Therefore, we recommend washing away the Blocking Solution instead of just draining it away as in other procedures. We guarantee that the product will meet all requirements described from its shipping date until its expiry date, as long as the product is correctly stored and utilized. No additional guarantees can be given. Under no circumstances shall Sanbio be liable for any damages arising out of the use of the reagent provided
Precautions:
Use by qualified personnel only. Wear protective clothing to avoid eye, skin or mucous membrane contact with the reagents. In case of a reagent coming into contact with a sensitive area, wash the area with large amounts of water. Microbial contamination of the reagents must be avoided, since otherwise non-specific staining might appear. ProClin is used for stabilisation. A material safety data sheet is available upon request.
CD44, also designated Pgp-1 or H-CAM, is a broadly distributed 85 kD protein. Amongst haematopoietic cells CD44 is expressed on B and T lymphocytes, monocytes and neutrophils. Other CD44-positive cell types include epithelial cells, glial cells, fibroblasts and myocytes. This broad distribution suggest a general role in cell-cell and cell-matrix adhesion. CD44 is involved in T cell activation and adhesion. Triggering of CD44 increases homotypic T cell aggregation mediated via LFA-1. CD44 is linked to the cytoskeleton. Furthermore CD44 has an accessory role in lymphocyte adhesion to high endothelial venules.
CD11b is an alpha chain integrin that is expressed as heterodimer noncovalently associated with CD18. CD11b is also designated as CR3, Mac-1, Mo-1 in integrin alphaM subunit and has a molecular weight of 165-170 kDa. The protein mediates PMN and monocytes adherence to endothelium and subsequently PMN extravasation to sites of inflammation. CD11b/CD18 interacts with both matrix and cell surface proteins including the complement component iC3b, extracellular matrix proteins and the third extracellular domain of CD54. CD11b is expressed on human NK-cells, monocytes, granulocytes, T-cells, B-cells and dendritic cells. After activation the expression is upregulated. Bear-1 reacts with the CD11b alpha chain of the CD11b/CD18 (p165, 95) integrin heterodimer complex.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
BEAR-1
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Keizer; G et al. Eur J Immunol 1985; 15: 1142
References 2:
Watanabe, A et al Int Arch Allergy Immunl 1992, 98: 308
Fibronectin receptor, also designated VLA-5, is a 130/150 kDa protein. The protein functions as a receptor for fibronectin and mediates binding of B and T lymphocytes to fibronectin. Fibronectin is an extracellular matrix glycoprotein that functions in cell adhesion and migration in wound healing, embryonic development and malignant transformation. The fibronectin receptor is expressed on monocytes and monocytoid cell lines, leukocytes, memory T cells, fibroblasts, platelets and muscle cells.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
NKI-SAM1
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
te Velde; A et al. J Immunol 1988; 140: 1548
References 2:
Danen, E et al Soc for Biochem and Mol Biol 1995, 270: 21612
CD3 is presented on all resting and activated human T-cells, on T-leukemia cells and a proportion of human thymocytes. CD3 plays an important role in the assembly and expression of the T-cell receptor complex. Furthermore it functions as a signal transductor. SPV-T3b reacts with human CD3 also designated as T3 with a molecular weight of 20-26 kDa. SPV-T3b recognizes also the T3 molecular complex.
The antibody recognizes a heterodimeric glycoprotein of 145, 185 kD which has been identified as NCAM (Neural Cell Adhesion Module). Detection of NCAM on cultured cells and in frozen tissue sections. The antibody is further useful for studies on neoplasms of the lung and the nervous system.
This antibody recognizes a high molecular weight proteoglycan with a molecular weight of > 450 kD (chondroitin sulfate) and 250 kD (core protein). The antibody reacts strongly with melanoma cells derived from cell lines and short term cultures and reacts preferentially with melanoma cells in frozen tissue sections. The antibody can also be used to detect melanoma lesions in vivo. Crossreactivity: The antibody reacts with most naevi and perineurium, and shows weak reactivity with hair follicles.
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.
Specificity:
Heavy (Gamma) chains on Hamster IgG and light chains on all Hamster Immunoglobulins
Cross Reactivity:
No reactivity to non-immunoglobulin hamster serum proteins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
This antibody recognizes a molecular complex consisting of two bands with MW of 150 kD and 90 kD respectively (reduced 4 subunits 120 kD, 95 kD, 29 kD and 25 kD). The antibody reacts strongly with melanoma cells derived from cell lines and short term cultures and melanoma cells in frozen tissue sections. The antigen detected by this antibody is found to be associated with the adhesion, spreading and motility of human cultured melanoma cells. Cross reactions: The antibody reacts with a proportion of naevi and with endothelial cells of small vessels.
Affinity purified using solid phase Bovine IgG (H&L)
Purity:
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Rabbit
Immunogen:
Purified Bovine IgG, whole molecule
Buffer:
PBS, 1% BSA & 0.1% proclin 150
Preservative:
0.1% (v/v) Kathon CG
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on bovine IgG · light chains on all bovine immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin bovine serum immunoglobulins
Country Of Origin:
Rabbit serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Melanoma associated antigen (MAA) is dispersed in the cytoplasma of melanoma cells, and is more concentrated inside vacuoles and sometimes on the melanosomes. Occasionally the antigen is seen on the cell surface. The antigen is actively shed from living cells. Although the antigen is associated with melanomas, it is not codistributed with the tyrosinase activity associated with melagonesis. The antigen shows codistribution with cathepsin D, which is a marker for lysosomal functions. The antibody NKI/beteb recognizes a (pre)melanosomal 100 kD and 7 kD antigen (glycoprotein). The antibody reacts with melanomas, clear cells sarcomas (melanoma of soft tissue), nevocellular nevi, and normal melanocytes. Except for one case of non-Hodgkin's lymphoma in which macrophages were positive, no reactions with other tumors or tissues have been observed.
The PRAME [IHC092] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissues within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC092
GMDN Code:
65250
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Melanoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Siglec-15 (Sialic acid binding Ig-like lectin 15), is a member of the family of Siglecs cell surface proteins that bind sialic acid. Involved in cell adhesion and signalling, Siglec-15 expression in dendritic and macrophage cells interacts with DAP10 and DAP12 and binds to sialylated glycoproteins
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Applications:
IHC
Clone number:
IHC096
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Prostate, Tonsil
Buffer:
Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
The MCT4 [IHC124] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissues within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Applications:
IHC
Clone number:
IHC124
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Cervical Cancer
Buffer:
Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
The D-Dimer [IHC085] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissues within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Applications:
IHC
Clone number:
IHC085
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Glioma
Buffer:
Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
Alpha-fetoprotein (AFP) is present in fetal plasma, and it binds e.g. copper, nickel, and bilirubin. Measuring of alpha-fetoprotein level in amniotic fluid can reveal severe fetal defects. In adults, elevated AFP concentrations in the plasma can indicate hepatocellular carcinoma or teratoblastoma. In some individuals, hereditary persistance of alpha-fetoprotein can be observed without any obvious pathology.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
purified alpha-Fetoprotein
Applications:
IHC,ELISA,RIA,WB
Additional Info:
The mouse monoclonal antibody AFP-11 reacts with human alpha-Fetoprotein (AFP), a 70 kDa oncofetal antigen. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus.
Clone number:
AFP-11
Antibody Isotype:
IgG1
Application Details:
ELISA: The antibody AFP-11 has been tested as the capture antibody in a sandwich ELISA for analysis of human alpha-fetoprotein in combination with antibody AFP-01 (cat. no. 11-259-C100). Immunohistochemistry (paraffin sections): Recommended dilution: 10 ?g/ml; positive tissue: hepatocellular carcinoma; heat mediated antigen retrieval (sodium citrate buffer); mAb incubation 1 hour / RT, detection DAB.
Choriogonadotropin (hCG) is a protein of the molecular weight about 40 kDa. It belongs to the glycoprotein hormone family together with lutropin (LH), follitropin (FSH) and thyrotropin (TSH). Choriogonadotropin is synthesised by trophoblastic cells of the placenta during pregnancy and stimulates the growth of corpus luteum. The other hormones are produced by anterior pituitary gland. All these hormones are structurally related, being composed of two noncovalently associated subunits alpha and beta.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Human chorionic gonadotropin.
Applications:
ICC,ELISA
Additional Info:
The antibody HCG-61 reacts with beta-subunit of human chorionic gonadotropin, a 40 kDa hormone. hCG belongs to the glycoprotein hormone family together with lutropin (LH), follitropin (FSH) and thyrotropin (TSH). hCG is synthesised by trophoblastic cells of the placenta during pregnancy and stimulates the growth of corpus luteum. The HCG-61 antibody reacts with association constant 5.1 x 1010 l/mol. Following cross-reactivity (%) was determined by classic double-antibody RIA using unlabelled hormones: beta-hCG (77), alpha-hCG (1.3), hLH (0.86), hFSH (< 0.5), hTSH (< 0.5).
Herpes simplex virus (HSV) is a virus that manifests itself in two common viral infections. There are actually two types of herpes simplex virus, HSV1 and HSV2. These are very similar in many ways, and both can cause either oral herpes or genital herpes. HSV1 - most commonly develops into oral herpes infecting the lips (fever blisters or cold sores). HSV1 can also infect the genital area causing sores to develop. HSV2 - generally infects the genital area (genital herpes); however, HSV2 can also infect the mouth.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Exctract of HSV-2 infected VERO (green monkey kidney) cells.
Applications:
WB,ICC,ELISA
Additional Info:
The antibody T303 recognizes gG antigen of HSV2 (Herpes Simplex Virus type 2), a dsDNA virus, member of Herpesviridae family.
Thyrotropin (hTSH) promotes the growth of the thyroid gland in the neck and stimulates it to produce more thyroid hormones. hTSH is composed of two subunits - alpha nad beta.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Human thyrotropin.
Applications:
ICC,ELISA,RIA
Additional Info:
The antibody TSH-51 reacts with human thyroid stimulating hormone (hTSH, thyrotropin), a glycoprotein hormone produced by the anterior pituitary gland cells in response to signals from the hypothalamus gland in the brain. The TSH-51 antibody reacts with association constant 5.5 x 1010 l/mol. Following cross-reactivity expressed as binding of labelled hormone (% of total) was determined by solid phase RIA with excess of the antibody TSH-51: hTSH (68.6), hCG (0.03), hLH (2.99), hFSH (0.66).
Clone number:
TSH-51
Antibody Isotype:
IgG2a
Application Details:
RIA: The antibody TSH-51 is suitable in combination with the antibody TSH-116 for immunometric assays in the screening of neonatal hypothyroidism.
Thyrotropin (hTSH) promotes the growth of the thyroid gland in the neck and stimulates it to produce more thyroid hormones. hTSH is composed of two subunits - alpha nad beta.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Human thyrotropin.
Applications:
ELISA,RIA
Additional Info:
The antibody TSH-116 reacts with human thyroid stimulating hormone (hTSH, thyrotropin), a glycoprotein hormone produced by the anterior pituitary gland cells in response to signals from the hypothalamus gland in the brain. The TSH-116 reacts with association constant 1.1 x 1011 l/mol. Following cross-reactivity expressed as binding of labelled hormone (% of total) was determined by solid phase RIA with excess of the antibody TSH-116: hTSH (78.9), hCG (20.3), hLH (23.2), hFSH (29.9).
Clone number:
TSH-116
Antibody Isotype:
IgG2a
Application Details:
RIA: The antibody TSH-116 is suitable in combination with the antibody TSH-51 for immunometric assays in the screening of neonatal hypothyroidism.
Alpha-fetoprotein (AFP) is present in fetal plasma, and it binds e.g. copper, nickel, and bilirubin. Measuring of alpha-fetoprotein level in amniotic fluid can reveal severe fetal defects. In adults, elevated AFP concentrations in the plasma can indicate hepatocellular carcinoma or teratoblastoma. In some individuals, hereditary persistance of alpha-fetoprotein can be observed without any obvious pathology.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
purified alpha-Fetoprotein
Applications:
IP,ELISA,WB,ICC
Additional Info:
The mouse monoclonal antibody AFP-01 reacts with human alpha-Fetoprotein (AFP), a 70 kDa oncofetal antigen. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus.
Clone number:
AFP-01
Antibody Isotype:
IgG1
Application Details:
ELISA: The antibody AFP-01 has been tested as the detection antibody in a sandwich ELISA for analysis of human alpha-fetoprotein in combination with antibody AFP-11 (cat. no. 11-384-C100). Western blotting: Positive material: Hep G2 human hepatocellular carcinoma cell line; both non-reducing and reducing conditions. Immunoprecipitation: Interaction of the antibody AFP-01 with AFP is dependent on the presence of calcium ions (strongly inhibited by chelating agents). Such characteristics of the antibody can be exploited for immunoaffinity purification of APF under mild elution conditions.
Herpes simplex Vvrus (HSV) is a virus that manifests itself in two common viral infections. There are actually two types of herpes simplex virus, HSV1 and HSV2. These are very similar in many ways, and both can cause either oral herpes or genital herpes. HSV1 - most commonly develops into oral herpes infecting the lips (fever blisters or cold sores). HSV1 can also infect the genital area causing sores to develop. HSV2 - generally infects the genital area (genital herpes); however, HSV2 can also infect the mouth.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Exctract of HSV-1 infected VERO (green monkey kidney) cells.
Applications:
WB,ICC,ELISA
Additional Info:
The antibody T96 recognizes gC antigen of HSV1 (Herpes Simplex Virus type 1), a dsDNA virus, member of Herpesviridae family.
Herpes simplex virus (HSV) is a virus that manifests itself in two common viral infections. There are actually two types of herpes simplex virus, HSV1 and HSV2. These are very similar in many ways, and both can cause either oral herpes or genital herpes. HSV1 - most commonly develops into oral herpes infecting the lips (fever blisters or cold sores). HSV1 can also infect the genital area causing sores to develop. HSV2 - generally infects the genital area (genital herpes); however, HSV2 can also infect the mouth.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Exctract of HSV-1 infected VERO (green monkey kidney) cells.
Applications:
ICC,ELISA
Additional Info:
The antibody T111 recognizes gB antigen of both HSV1 and HSV2 (Herpes Simplex Virus type 1 and 2), a dsDNA virus, member of Herpesviridae family.
The antibody 7LE recognizes Lewis<sup>a</sup> blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, expressed in colonic epithelial cells. Lewis<sup>a</sup> may be useful for detection of gastrointestinal, pancreatic and colorectal tumors.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Mucin isolated by size-exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio´s classification).
Applications:
IHC,WB,ELISA
Additional Info:
The mouse monoclonal antibody 7LE recognizes Lewisa blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, expressed on colonic epithelial cells. Lewisa may be useful for detection of gastrointestinal, pancreatic and colorectal tumors.
The antibody 2-25LE reacts with Lewis<sup>b</sup> blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Mucin isolated by size exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio´s classification).
Applications:
IHC,WB,ELISA
Additional Info:
The antibody 2-25LE reacts with Lewisb blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, detected on the surface of erythrocytes, certain epithelial cells, and in secretions of certain individuals.
The antibody E1-2.5 recognizes repetitive Gly-Ala region of the Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA-1). EBNA-1 is the only viral protein consistently expressed in all malignancies associated with EBV.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Applications:
ICC,ELISA
Additional Info:
The mouse monoclonal antibody E1-2.5 recognizes repetitive Gly-Ala region of the Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA-1). EBNA-1 is the only viral protein consistently expressed in all malignancies associated with EBV.
HBsAg (hepatitis B surface antigen) is a marker of infectivity. Its presence indicates either acute or chronic HBV infection.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Purified HbsAg from human plasma.
Applications:
ICC,ELISA
Additional Info:
The antibody HB5 recognizes following Hepatitis B virus (HBV) subtypes: ayw2, ayw3, ayw4, ayr, adw2, adw4, adrq+, adrq-. Hepatitis B surface antigen (HBsAg) is a marker of infectivity. Specificity of the antibody HB5 was verified by ELISA on panel of virus subtypes identified on International Workshop on HBsAg Subtypes (Paris, April 1975). The antibody HB5 does not cross-block with the antibody HB3.
HBsAg (hepatitis B surface antigen) is a marker of infectivity. Its presence indicates either acute or chronic HBV infection.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Purified HBsAg from human plasma.
Applications:
ICC,ELISA
Additional Info:
The antibody HB3 recognizes following Hepatitis B virus (HBV) subtypes: ayw2, ayw3, ayw4, ayr, adw2, adw4, adrq+, adrq-. Hepatitis B surface antigen (HBsAg) is a marker of infectivity. Specificity of the antibody HB3 was verified by ELISA on panel of virus subtypes identified on International Workshop on HBsAg Subtypes (Paris, April 1975). The antibody HB3 does not cross-block with the antibody HB5.
The antibody HP-03 reacts with horseradish peroxidase. It is suitable for PAP detection system.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Horseradish peroxidase (Grade I)
Applications:
WB,ICC,ELISA
Additional Info:
The antibody HP-03 reacts with horseradish peroxidase. It is suitable for PAP detection system.
Clone number:
HP-03
Antibody Isotype:
IgG1
Application Details:
Immunocytochemistry: The antibody HP-03 was successfully used for staining of formaldehyde-fixed, Triton-permeabilized cells transfected with HRP gene.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Rabbit
Immunogen:
Purified Hamster IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20°C.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on hamster IgG · light chains on all hamster immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin hamster serum immunoglobulins
Country Of Origin:
Rabbit serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Trademark:
DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Rabbit
Immunogen:
Purified goat IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20°C.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on goat IgG · light chains on all goat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin goat serum immunoglobulins · IgG from human, mouse or rat
Country Of Origin:
Rabbit serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Trademark:
DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Rabbit
Immunogen:
Purified goat IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20°C.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on goat IgG · light chains on all goat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin goat serum immunoglobulins · IgG from human, mouse or rat
Country Of Origin:
Rabbit serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Trademark:
DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Rabbit
Immunogen:
Purified goat IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20°C.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on goat IgG · light chains on all goat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin goat serum immunoglobulins · IgG from human, mouse or rat
Country Of Origin:
Rabbit serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Trademark:
DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Human IgA, (α chain)
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20°C.
Specificity:
Based on IEP, this antibody reacts with: · heavy (α) chains on human IgA
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin human serum immunoglobulins · light chains on all human immunoglobulins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Accession Number:
CAA10821
Trademark:
DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Unlike human, the murine protein is expressed only as a transmembrane isoform. Also CD32 (FcgammaRII) is a low affinity receptor for IgG, but its affinity is lower than that of CD16. These receptors are expressed on monocytes/macrophages, NK cells, granulocytes, mast cells, dendritic cells, and B cells. Their role is to mediate adaptive immune responses through binding the antibody-antigen immunocomplexes, but their effect on the particular cell differs according to the cell type.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Murine pre-B cells
Applications:
FC
Additional Info:
The rat monoclonal antibody 93 recognizes a common extracellular epitope of murine CD16 (FcgammaRIII) and CD32 (FcgammaRII), the low affinity receptors for IgG.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Mouse IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20°C.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum proteins · bovine, goat, human, rabbit or rat IgG
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Accession Number:
AAA51107
Trademark:
DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
B10 mouse spleen cells enriched for T cells
Applications:
FC
Additional Info:
The rat monoclonal antibody M1/70 detects an extracellular epitope of CD11b (integrin alphaM subunit), a type I transmembrane protein mainly expressed on monocytes/macrophages, granulocytes and NK-cells, which associates with CD18 to form Mac-1 integrin that plays important role in cell-cell interactions.
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkynje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Mouse BM10-37 cytotoxic T lymphocytes
Applications:
FC
Additional Info:
The Armenian hamster monoclonal antibody 145-2C11 reacts with an extracellular epitope of murine CD3 (epsilon subunit). This antibody is commonly used as a phenotypic marker for murine T cells.
ICE1 (Inducer of CBF expression 1) is a negative regulator in brassinosteroid signal transduction pathway important for plant growth. Phosphorylates and increases the degradation of BZR1 and BZR2/BES1 by the proteasome. Phosphorylates BHLH150, beet curly top virus C4 and tomato golden mosaic virus AC4 on threonine and serine residues. Upon brassinosteroid signaling, inhibits stomatal development by phosphorylating and inhibiting the MAPKK kinase YDA and the MAPK kinases MKK4 and MKK5.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica napus, Brassica rapa, Capsella bursa-pastoris, Capsella rubella, Eutrema salsugineum, Noccaea caerulescens Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana ICE1 sequence, UniProt: Q9LSE2#Q9LSE2-2, TAIR: AT3G26744. The peptide is not conserved in protein coded by SCRM2 gene. UniProt: A0A1P8AMV9
Prostate-specific antigen (PSA) is a protein produced by normal prostate cells. This enzyme participates in the dissolution of the seminal fluid coagulum and plays an important role in fertility. The highest amounts of PSA are found in the seminal fluid; some PSA escapes the prostate and can be found in the serum. This serum component has been used to track the response to therapy in men with prostate cancer.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
30 kDa protein fraction prepared from human seminal plasma
Applications:
WB,IHC,ELISA
Additional Info:
The mouse monoclonal antibody A67-B/E3 reacts with prostate-specific antigen (PSA), a protein produced by normal prostate cells. The highest amounts of PSA are found in the seminal fluid.
Ly6G is a component of the myeloid differentiation antigen Gr-1, together with Ly6C. Ly6G is a good marker for detection of peripheral neutrophils. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. Physiological role of Ly6G remains still unclear. Its treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Murine granulocytes
Applications:
FC
Additional Info:
The rat monoclonal antibody RB6-8C5 detects Ly6G component of Gr-1 antigen, a commonly used surface marker of neutrophils.
The antibody SC-05 reacts with 80 kDa human secretory component glycoprotein (both free and bound in secretory IgA). Recognized glycoprotein is specific membrane marker of glandular carcinomas.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Affinity-purified secretory component from human colostrum.
Applications:
IP,WB,IHC
Additional Info:
The antibody SC-05 reacts with 80 kDa human secretory component glycoprotein (both free and bound in secretory IgA). Recognized glycoprotein is specific membrane marker of glandular carcinomas.
Clone number:
SC-05
Antibody Isotype:
IgG1
Application Details:
Immunohistochemistry (frozen sections): Examples of positive human tissues: secretory mucosa of gastrointestinal and respiratory tract, epithelia of salivary glands, endometrium, endocervix, kidney, prostate, mammary gland. Recommended dilution: 2-8 ?g/ml.
CO (CONSTANS) is a putative transcription factor which is involved in the long day flowering pathway and has been implied to control mediation between circadian clock and flowering. It regulates flowering time by interactions with SOC1 (suppressor of CO 1), TF1 (terminal flower 1), and FT (flowering locus T). Furthermore it is involved in proline and ethylene biosynthesis.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Goat
Species Reactivity:
Arabidopsis thaliana (recombianant CO)
Expected Species:
Camelina sativa, Chrysanthemum sp.Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from protein sequence of Arabidopsis thaliana CO protein, UniProt: Q39057, TAIR: At5g15840
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody is recognizing recombinant CO protein. Detection conditions in endogenous extract remain to be determined.Recommended protocol for CO protein extraction Serrano-Bueno et al. 2020. Detection has to be done on a nuclear extract preparation in the evening on a Long Day that is the moment when CO protein is more stable.
Goat anti-Human IgG Fc, F(ab)'2 Fragment, min. cross-reactivity to Human IgA/IgM, Bovine, Horse, Mouse, Rabbit Serum is a secondary antibody, which binds to human IgG Fc, F(ab)'2 Fragment in immunological assays.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Antibody purity is > 90% based on SDS-PAGE Small amounts of intact IgG may be present.Affinity purified using solid phase Human IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on human IgG .Based on IEP, no reactivity is observed to: • non-immunoglobulin human serum immunoglobulins • light chains on all human immunoglobulins • human IgA or IgM • serum proteins from bovine, mouse, or rabbit .
BIN2 (Brasinosteroid insensitive 2) plays an important role in regulating brassinosteroid signalling which is important for plant growth. It is also implied to mediate auxing signalling pathway. Phosphorylation of BZR1 and BZR2/BES2 by BIN2 increases degradation of these proteins by the proteasome.Alternative names:ATSK21, DWARF 12, DWF12, SHAGGY-LIKE KINASE 21, SK21, UCU1, ULTRACURVATA 1
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Hu et al. (2023) Spatiotemporal formation of the large vacuole regulated by the BIN2-VLG module is required for female gametophyte development in Arabidopsis [published online ahead of print, 2023 Jan 17]. Plant Cell. 2023;koad007. doi:10.1093/plcell/koad007
ANN-1 | Annexin-1 displays peroxidase activity and may counteract oxidative stress. Alternative names: ANNAT1, ANX23-ATH, ATOXY5, OXY5, Annexin D1, AnnAt1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide derived from Arabidopsis thaliana Annexin-1, UniProt Q9SYT0, TAIR AT1G35720
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Host:
Rabbit
Immunogen:
Purified goat IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on goat IgG · light chains on all goat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin goat serum immunoglobulins
Country Of Origin:
Rabbit serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Rabbit
Immunogen:
Purified goat IgG, whole molecule
Buffer:
PBS, 1% BSA & 0.1% proclin 150
Preservative:
0.1% (v/v) Kathon CG
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on goat IgG · light chains on all goat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin goat serum immunoglobulins · IgG from human, mouse or rat
Country Of Origin:
Rabbit serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
CD38 is a widely expressed 45 kDa transmembrane glycoprotein with a short cytoplasmic tail (aa 121), a transmembrane domain (aa 2242) and an extracellular domain (aa 43300) [1]. It is a multifunctional ectoenzyme catalysing multiple reactions and also has a role in signal transduction and calcium signaling.
In the brain CD38 is expressed in neurons, astrocytes and microglial cells and expression was found to increase under neuroinflammatory conditions indicating that it may have a role in the regulation of neuroinflammation. In Alzheimers disease, CD38 immunoreactivity is seen in intracellular tangles and neuropil threads [2].
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal Antibody
Format:
50 µg in 50 µl Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
Storage Temp:
Store at -20°C. Avoid freeze / thaw cycles.
Host Animal:
Rabbit
Species Reactivity:
Human, Rat
Immunogen:
A synthetic peptide from the C-terminal region of human CD38
If you would like further information regarding the immunogen used in the production of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
[1] Malavasi, F.; Deaglio, S.; Funaro, A.; Ferrero, E.; Horenstein, A.L.; Ortolan, E.; Vaisitti, T.; Aydin, S. Evolution and Function of the ADP Ribosyl Cyclase/CD38 Gene Family in Physiology and Pathology. Physiol. Rev. 2008, 88, 841886. [2] Otsuka K., Mizuguchi M., Aizawa T., Haga S., Sato M., Inoya H., Namba Y., Machinami R. Immunoreactivity in Alzheimers neurofibrillary tangles (abstract) Brain Pathol. 1994;4:558.
TREM2 (Triggering receptor expressed on myeloid cells 2) is a cell surface transmembrane glycoprotein with an immunoglobulin like extracellular domain and a cytoplasmic tail [1]. It is expressed in myeloid cells including dendritic cells, granulocytes, and tissue-specific macrophages such as osteoclasts. In the brain, TREM2 is only expressed by microglia and in the central nervous system expression of TREM2 varies with higher expression in the hippocampus, spinal cord and white matter.
TREM2 plays a complex role in neuroinflammation with expression of TREM2 being upregulated in pathological conditions such as Parkinson's disease, Amyotrophic lateral sclerosis (ALS), stroke, traumatic brain injury and Alzheimer's Disease [2]. TREM2 has also been shown to have a role in potential mechanisms linking urban air pollution to Alzheimer's Disease through its involvement in the regulation of neuroinflammation [3].
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal
Format:
50 µg in 50 µl PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
If you would like us to check if this antibody is likely to bind to this protein from a different species please contact us. We are happy to check for you.
Immunogen:
KLH conjugated synthetic peptide from the N-terminal region of human TREM2
If you would like further information regarding the immunogen used in the production of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
TREM-2, TREM2a, TREM2b, TREM2c, Trggering receptor expressed on myeloid cells 2, Trggering receptor expressed on myeloid cells 2a, Triggering receptor expressed on monocytes 2
Research Areas:
Neuroscience
NS Reagents Product Area:
Neuroscience
Molecular Weight:
25kDa (Intended as a general guide and does not allow for all isoforms and species variations)
Subcellular location:
Cell membrane, Secreted
Purification:
Affinity purification
References:
[1] Bouchon A, Dietrich J, Colonna M. Cutting edge: inflammatory responses can be triggered by TREM-1, a novel receptor expressed on neutrophils and monocytes. J Immunol. 2000;164:49915.
[2] Gratuze, M., Leyns, C.E.G. & Holtzman, D.M. New insights into the role of TREM2 in Alzheimers disease. Mol Neurodegeneration 13, 66 (2018).
[3] Hendrik J. Greve, Christen L. Mumaw, Evan J. Messenger, Prasada R. S. Kodavanti, Joyce L. Royland, Urmila P. Kodavanti and Michelle L. Block. Diesel exhaust impairs TREM2 to dysregulate neuroinflammation. J Neuroinflammation. 2020; 17: 351.
Lysosomal acid glucosylceramidase (GBA or Glucocerebrosidase) is the lysosomal hydrolase that hydrolyzes glucosylceramide (GC) and glucosylsphingosine (GS) to ceramide and sphingosine. It is a 536-amino-acid membrane-associated protein with a 39-amino-acid leader sequence that is cleaved to produce a 497-amino-acid mature protein.
Mutations in the GBA gene cause Gaucher disease, a lysosomal storage disease characterised by an accumulation of glucocerebrosides. Patients with Gaucher disease and heterozygous carriers are at increased risk of developing Parkinson's disease and Dementia with Lewy Bodies.
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal
Format:
50 µg in 50 µl PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
If you would like further information regarding the immunogen used in the production of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
Phospholipase C-gamma2 (PLCG2) PLCG2 is an enzyme mainly expressed in immune cells (including microglia) which are involved in innate immunity. It is involved in the transmembrane transduction of immune signals that determine the fate and function of various immune cell types.
PLCG2 has higher expression levels in pathologically afected brain regions in Alzheimers disease indicating that the immune system may play a key role in the development of Alzheimers disease and a polymorphism in phospholipase C-gamma 2 (PLCG2) has been reported to be protective against late onset Alzheimers disease (LOAD) [1].
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal Antibody
Format:
50 µg in 50 µl Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
Storage Temp:
Store at -20°C. Avoid freeze / thaw cycles.
Host Animal:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Expected Species:
Cat, Dog, Chimpanzee, Pig, Bovine
Immunogen:
Partial length recombinant human PLCG2 from the N-terminal region
If you would like further information regarding the immunogen used in the production of this antibody or have a query about if this antibody will bind to your protein/species please contact us and we can do the analysis for you.
[1] Sims R, van der Lee SJ, Naj AC, Bellenguez C, Badarinarayan N, Jakobsdottir J et al (2017) Rare coding variants in PLCG2, ABI3, and TREM2 implicate microglial-mediated innate immunity in Alzheimers disease. Nat Genet 49:13731384.
Histamine is an important messenger molecule and neuromodulator with four known G protein-coupled receptors (GPCRs), H1, H2, H3 and H4. Histamine receptor H3 (HRH3) is a presynaptic autoreceptor that regulates histamine release from histaminergic neurons via negative feedback [1] as well as a heteroreceptor that regulates the release of other neurotransmitters [2,3,4]. It is also expressed postsynaptically in the modulation of dopamine signalling.
HRH3 is expressed predominantly in the cortex, thalamus, hypothalamus, hippocampus, amygdala and basal ganglia. Dysregulated central histaminergic signalling has been associated with a number of psychiatric disorders including sleep disorders, ADHD, Alzheimer's disease, Parkinson's disease and schizophrenia.
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal Antibody
Format:
50 µg in 50 µl Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
Storage Temp:
Store at -20°C. Avoid freeze / thaw cycles.
Host Animal:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Expected Species:
Guinae Pig, Cat
Immunogen:
A synthetic peptide from the C-terminal region of human HRH3
If you would like further information regarding the immunogen used in the production of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
Antibody Isotype:
IgG
Application Details:
WB 1:2000-5000. IHC 1:10-100.
Category:
Primary Antibodies
Other names:
HH3R, G-protein coupled receptor 97, GPCR97, H3R
Research Areas:
Neuroscience
NS Reagents Product Area:
Neuroscience
Molecular Weight:
49kDa
Subcellular location:
Plasma Membrane
Purification:
Affinity purification
References:
[1] Morisset S, Rouleau A, Ligneau X, Gbahou F, Tardivel-Lacombe J, Stark H, Schunack W, Ganellin CR, Schwartz JC, Arrang JM. High constitutive activity of native H3 receptors regulates histamine neurons in brain. Nature. 2000; 408:860-4. [2] Schlicker E, Betz R, Gothert M. Histamine H3 receptor-mediated inhibition of serotonin release in the rat brain cortex. Naunyn Schmiedebergs Arch Pharmacol. 1988; 337:588-90. [3] Schlicker E, Fink K, Hinterthaner M, Gothert M. Inhibition of noradrenaline release in the rat brain cortex via presynaptic H3 receptors. Naunyn Schmiedebergs Arch Pharmacol. 1989; 340:633-8. [4] Schlicker E, Fink K, Detzner M, Gothert M. Histamine inhibits dopamine release in the mouse striatum via presynaptic H3 receptors. J Neural Transm Gen Sect. 1993; 93:1-10.
Superoxide dismutase 1 (SOD1) is one of three superoxide dismutases with SOD1 being the principal cytoplasmic superoxide dismutase in humans. It binds copper and zinc ions for its activity and stability and plays a major role in redox potential regulation. It catalyses the transformation of the superoxide anion (O2?) into hydrogen peroxide. SOD1 variants are a common cause of familial amyotrophic lateral sclerosis (ALS).
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal
Format:
50 µg in 50 µl PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
If you would like us to check if this antibody is likely to bind to this protein from a different species please contact us. We are happy to check for you.
If you would like further information regarding the immunogen used in the production of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
Antibody Isotype:
IgG
Application Details:
WB 1:500-2000. IHC 1:50-200.
Category:
Primary Antibodies
Other names:
ALS, ALS1, HEL-S-44, IPOA, SOD, hSod1, homodimer
Research Areas:
Neuroscience
NS Reagents Product Area:
Neuroscience
Molecular Weight:
16kDa (Intended as a general guide and does not allow for all isoforms and species variations)
Goat anti-Mouse IgG (H&L), F(ab)'2 fragment, min. cross-reactivity to bovine, horse, human, pig or rabbit serum protein is a FITC conjugated secondary antibody which binds to mouse IgG (H&L), F(ab)'2 Fragment in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store non-diluted antibody at 2-8°C. For storage at -20 C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.
Antibody purity is > 90% based on SDS-PAGE Small amounts of intact IgG may be present.Affinity purified using solid phase Mouse IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free.Based on IEP, this antibody reacts with: • heavy (γ) chains on mouse IgG • light chains on all mouse immunoglobulins .Based on IEP, no reactivity is observed to: • non-immunoglobulin mouse serum immunoglobulins • serum proteins from bovine, horse, human rabbit or swine .
For reconstitution add 1,1 ml of sterile water,Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Mouse IgM.Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.1% (v/v) Kathon CG is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on mouse IgM • light chains on all mouse immunoglobulins .Based on IEP, no reactivity is observed to: • non-immunoglobulin mouse serum proteins .
Goat anti-Mouse IgG (H&L), F(ab)'2 fragment, min. cross-reactivity to bovine, horse, human, pig or rabbit serum protein is a secondary antibody which binds to mouse IgG (H&L), F(ab)'2 Fragment in immunological assays.
For reconstitution add 0,55 ml of sterile water,Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Antibody purity is > 90% based on SDS-PAGE Small amounts of intact IgG may be present.Affinity purified using solid phase Mouse IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free.Based on IEP, this antibody reacts with: • heavy (γ) chains on mouse IgG • light chains on all mouse immunoglobulins .Based on IEP, no reactivity is observed to: • non-immunoglobulin mouse serum immunoglobulins • serum proteins from bovine, horse, human rabbit or swine .
Goat anti-Human IgG Fc, TRITC conjugated, min. cross-reactivity to bovine, horse, mouse or rabbit serum proteins or mouse IgG1 is a secondary antibody conjugated to TRITC, which binds to human IgG Fc in immunological assays.
For reconstitution add 1,1 ml of sterile water,Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Human IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on human IgG .Based on IEP, no reactivity is observed to: • non-immunoglobulin human serum immunoglobulins • light chains on all human immunoglobulins • human IgG F(ab)'2 fragment • mouse IgG1 • serum proteins from bovine, horse, mouse, or rabbit .
CNTF is a survival promoting factor for different types of neurons in vitro and in vivo. The essential structural features for the biological function of human CNTF were investigated by Thier, M. et al. They showed that deletion of 14 N-terminal and 18 C-terminal amino acids significantly increased bioactivity compared to wild-type CNTF. FUNCTION: CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. SUBUNIT: Homodimer. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Nervous system. PHARMACEUTICAL: CNTF is being tested under the name Axokine by Regeneron Pharmaceuticals for treatment of human motor neuron diseases, such as amyotrophic lateral sclerosis (ALS). As it induces substantial weight loss, preferentially of fat as opposed to lean body mass, it is being used for obesity treatment. SIMILARITY: Belongs to the CNTF family.
Product Type:
Protein
Format:
Lyophilized
Applications:
Cell Culture
Alternative Names:
Ciliary NeuroTrophic Factor
Biosensis Brand:
Biosensis®
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Qiping D et al. (2018) Mechanism and consequence of abnormal calcium homeostasis in Rett syndrome astrocytes. eLife. [Accepted Manuscript]. Williams EC et al. (2014) Mutant astrocytes differentiated from Rett syndrome patients-specific iPSCs have adverse effects on wild-type neurons. Hum Mol Genet. 2014 Jun 1;23(11):2968-80.
Storage:
Aliquot and keep at -20°C for long-term storage. For short term keep at 2-8°C. Avoid repetitive freeze/thaw cycles.
X63-saporin is an antibody conjugate comprising of the non-specific monoclonal IgG 1 antibody X63, chemically conjugated via a reducible disulfide bridge to the ribosome-inactivating protein saporin, purified from saponaria officinalis . Antibody clone X63 has no known binding ability, and thus can be used as negative control antibody. In combination with saporin, X63-saporin is a useful negative control for targeting IgG 1 -saporin conjugates such as MC192-saporin.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from a 1 mg/mL solution containing PBS pH 7.2-7.6 without preservative.
Host Animal:
Mouse
Species Reactivity:
Non-Reactive (Negative Control)
Immunogen:
Unknown, naturally isolated IgG that has no known binding target in mammals
Applications:
Negative Control
Clone number:
X63
Antibody Isotype:
IgG1, kappa
Application Details:
Negative control for targeting IgG1-saporin conjugates., for instance MC192-saporin. X63-saporin should be used at a concentration equal to that of the test antibody-saporin conjugate.
Biological Activity:
None. Routinely tested for absence of killing of rat C6 cells in vitro. Note that the primary use of X63-saporin is for in vivo applications.
Biosensis Brand:
Biosensis®
Conjugate:
Saporin
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
No staining has ever been identified with this immunoglobulin demonstrating its non-specific value as a control.
Storage:
Lyophilized product is shipped at ambient room temperature. Upon receipt, pulse-centrifuge the vial to collect solid that may be entrapped in the lid. After reconstitution, immediately prepare aliquots and keep the undiluted stock at -80°C for long-term storage. Avoid repeated thaw-freezing. For short-term storage, keep at 2-8°C for up to 2 weeks. it is recommended to handle this product under sterile conditions.
Purification:
Conjugate was purified by ion-exchange chromatography. Purity > 90% by non-reducing SDS-PAGE
Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons.
Product Type:
Protein
Format:
Lyophilized, When reconstituted in 0.5 mL sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
Applications:
Cell Culture
Alternative Names:
Beta-nerve growth factor; beta-NGF; NGFB;
Biosensis Brand:
Biosensis®
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Storage:
Lyophilized products should be stored at 2-8°C. Following reconstitution, short-term storage at 2-8°C is recommended and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Purification:
>95%, as determined by SDS-PAGE and visualized by silver stain
Stem Cell Factor (SCF) is a hematopoietic growth factor that stimulates the proliferation of mast cells. SCF also mediates cell-cell adhesion and acts synergistically with other cytokines. The actions of SCF are exerted by binding to the SCF Receptor (PE-1243-50) which results in receptor dimerization. SCF is a type I membrane glycoprotein with 5 potential N-linked glycosylation sites. Soluble SCF is produced by proteolytic cleavage of the extracellular domain.
Product Type:
Protein
Format:
Purified SCF consists of 0-50% carbohydrate by weight.
Lyophilized products should be stored at 2-8°C. Following reconstitution, short-term storage at 2-8°C is recommended and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Purification:
>95%, as determined by SDS-PAGE and visualized by silver stain
Rabbit anti-Tyrosine Hydroxylase (TH) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine, epinephrine and norepinephrine. Therefore the regulation of the TH enzyme represents the central means for controlling the synthesis of these important catecholamines. FUNCTION: Plays an important role in the physiology of adrenergic neurons. CATALYTIC ACTIVITY: L-tyrosine + tetrahydrobiopterin + O2 = 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin. COFACTOR: Fe(2+) ion. ENZYME REGULATION: Phosphorylation leads to an increase in the catalytic activity. PATHWAY: Catecholamine biosynthesis; first step. SUBUNIT: Homotetramer. PTM: In vitro, phosphorylation of Ser-19 increases the rate of Ser-40 phosphorylation, which results in enzyme opening and activation. SIMILARITY: Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. The presence of different DNA sequences at the TH locus confers susceptibility to various disorders of the brain including manic-depression and schizophrenia. Parkinson's disease is also considered a TH deficiency as low dopamine levels are a consistent neurochemical abnormality.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (SPRFIGRRQSLIEDARK) as part of human Tyrosine Hydroxylase (aa: 32-47) conjugated to KLH
Applications:
IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
<b>Immunohistochemistry (IHC):</b> 0.5-1 ug/mL. This is a superb antibody for detection of tyrosine hydroxylase containing neurons exhibiting an intense labelling with a negligible background. This antiserum has proven extremely useful for staining of catecholaminergic neurons. It stains nicely and intensely dendritic processes and fine nerve terminals.<br><b>Western Blotting (WB):</b> 0.5-2 ug/mL. This antibody demonstrates clear immunoreactivity for TH at 60 kDa in rat PC12 cell lysate and mouse brain homogenate. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
IHC on brain shows a pattern of staining specific for TH containing neurons. This antibody is known to react with rat. Cross reactivity with other species has not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
The Plus HRP Polymer anti-Rabbit is designed for the qualitative detection of antigens in fixed paraffinembedded tissue sections, in frozen tissue sections, and in cytological samples. It is developed for use in combination with mono- and polyclonal primary antibodies and sera obtained from rabbits. The reagent can be used for examining tissues fixed in different solutions, e.g. formalin (neutrally buffered), B5, Bouin, ethanol, or HOPE.
The purpose of the immunohistochemical staining is to make tissue and cell antigens visible. The Plus HRP Polymer anti-Rabbit is a highly sensitive detection reagent intended for use in immunohistochemistry and immunocytochemistry. The enzyme polymer in this kit consists of several molecules of secondary antibodies covalently bound to several molecules of horse radish peroxidase (HRP). Visualisation occurs via an enzyme-substrate reaction in the presence of a colourising reagent which permits microscopical analysis. The reagent is suitable for the detection of mono- and polyclonal primary antibodies and sera obtained from rabbits. In contrast to other detection techniques, which often use the streptavidin-biotin system the Plus HRP Polymer anti-Rabbit avoids the problem of background staining caused by endogenous biotin in the tissue.
Principle of method:
Paraffin-embedded tissue sections are first deparaffinised and rehydrated. Endogenous peroxidase activity in the tissue may cause non-specific staining. This enzyme activity can be blocked by incubation with 3% H2O2-solution (Peroxide block). Background staining caused by unspecific binding of the primary antibody or the secondary antibody in the HRP polymer is minimized by incubation with a protein blocking solution (Blocking Solution). This step can be omitted if the primary antibodies are diluted in an appropriate buffer. The next step is incubation with the specific primary antibody. After washing, the HRP-polymer is applied and incubated. Any excess of unbound HRP-polymer is thoroughly washed away after incubation. The addition of the chromogenic substrate starts the enzymatic reaction of the horse radish peroxidase which leads to colour precipitation where the primary antibody is bound. The colour can be observed with a light microscope. The chromogen used determines the colour. The chromogen AEC leads to the formation of a red-brown product of reaction at the place of the target antigen. The chromogen DAB forms a dark brown precipitate.
Reagents provided:
6 ml HRP-Polymer anti-Rabbit (Ready-to-use) Substrate systems recommended: Permanent AEC kit, AEC Single Solution, AEC Substrate kit, DAB Substrate kit, DAB High contrast kit Materials required but not supplied: Positive und negative control tissue Xylene or suitable substitutes Ethanol, distilled H2O 3% H2O2 solution Reagents for enzyme digestion or heat pre-treatment Wash buffer PBS or TBS PAP Pen Primary antibody (user-defined) Primary antibody diluent Negative control reagent Chromogenic substrate Counter stain solution Mounting medium Cover slips
Storage and handling:
The solution should be stored at 2-8°C without further dilution. Please store the reagent in a dark place and do not freeze it. Under these conditions the solution is stable up to the expiry date indicated on the label. They should not be used after the expiry date. A positive and a negative control have to be carried out in parallel to the test material. If you observe unusual staining or other deviations from the expected results which could possibly be caused by the kit reagents, please contact our technical support
Reagent preparation:
Reagents should be at room temperature when used. Deparaffinise and rehydrate paraffin-embedded tissue sections. Pre-treatment (optional) with HIER (Heat Induced Epitope Retrieval) or enzymatic digestion. Tissue sections have to be completely covered with the different reagents in order to avoid drying out.
Procedure:
1. Peroxide blocking (3 % H2O2 solution) 10 min. 2. Washing with wash buffer 1 x 2 min. 3. Blocking Solution (This step is optional.) 5 min. 4. Washing with wash buffer 1 x 2 min. 5. Primary antibody (optimally diluted) or negative control reagent 30-60 min. 6. Washing with wash buffer 3 x 5 min. 7. HRP-polymer anti-Rabbit 30 min. 8. Washing with wash buffer 3 x 2 min. 9. AEC or DAB (Controlling the colour intensity via light microscope is recommended.) 5-15 min. 10. Stopping the reaction with distilled H2O when the desired colour intensity is attained 11. Counterstaining and blueing 12. Mounting: aqueous with AEC, permanent with DAB or Permanent AEC
Expected results:
During the reaction of the substrate with horse radish peroxidase in the presence of a chromogen, a coloured precipitate is formed at the location of the bound primary antibody. This reaction only takes place if the target antigen is existent in the tissue. The chromogen used determines the colour of the precipitate. The analysis is carried out using a light microscope.
Trouble shooting:
If you observe unusual staining or other deviations from the expected results which could possibly be caused by the reagents, please read these instructions carefully, contact our technical support. No staining on an actually positive control slide: 1. Reagents were not used in the proper order. 2. Chromogenic substrate solution was too old. 3. Bleaching because chromogen and mounting medium are incompatible. 4. The antigen/epitope in the tissue was insufficiently accessible to the primary antibody. Try a pre-treatment such as heat pre-treatment or enzyme digestion. If you used a pre-treatment it should be extended. 5. Primary antibody not from rabbit, but from a different species. 6. The antigen/epitope was not stable in the fixation and/or pre-treatment procedure used. Try another fixation or pre-treatment. Weak staining: 1. Inadequate fixation or overfixation. 2. Incomplete deparaffinisation. 3. The antigen/epitope in the tissue was insufficiently accessible to the primary antibody. If you used heat pre-treatment or enzyme digestion it should be extended. 4. Excessive incubation with Blocking Solution or insufficient washing after this step. 5. Too much wash buffer remains on the slides after washing, diluting the reagents applied in the next step. 6. Incubation times were too short or primary antibody concentration too low. 7. Chromogenic substrate solution was too old. Non-specific background staining or overstaining: 1. Incomplete deparaffinisation. 2. Excessive tissue adhesive on slides. 3. Insufficient washing especially after the incubation with the enzyme polymer or the chromogenic substrate solution. These washings are critical. 4. Tissue was allowed to (partially) dry out with reagents on. 5. Unspecific binding of the primary antibody. Please use a Blocking Solution or dilute the primary antibody in appropriate diluents. 6. Incubation time of the primary antibody was too long or primary antibody concentration too high. 7. Incubation time of the chromogenic substrate solution was too long or reaction temperature too high (e.g. if temperature in the laboratory is high). 8. The substrate is metabolised by endogenous horse radish peroxidase in the tissue. Maybe the hydrogen peroxide solution used for blocking was inactivated
Quality Control:
We recommend carrying out a positive and a negative control with every staining run. The positive control permits the validation of appropriate processing of the sample. If the negative control has a positive result, this points to unspecific staining.
Performance characteristics:
Studies have been conducted to evaluate the performance of the kit reagents. The product has been found to be suitable for the intended use
Limitations of procedure:
Immunohistochemistry is a complex method in which histological as well as immunological detection methods are combined. Tissue processing and handling prior to immunostaining, for example variations in fixation and embedding or the inherent nature of the tissue can cause inconsistent results (Nadji and Morales, 1983). Endogenous peroxidase or pseudoperoxidase activity may cause non-specific staining. The enzyme activity is blocked by incubation with hydrogen peroxide solution. Tissues containing Hepatitis B Surface Antigen (HBsAg) may give false positive results with HRP (horse radish peroxidase) detection systems (Omata et al, 1980). Inadequate counterstaining and mounting can influence the interpretation of the results. The colour intensity of the reaction product can decrease with time, especially when exposed to light. Sanbio guarantees that the product will meet all requirements described from its shipping date until its expiry date, as long as the product is correctly stored and utilized. No additional guarantees can be given. Under no circumstances shall Sanbio be liable for any damages arising out of the use of the reagent provided.
Precautions:
Use by qualified personnel only. Wear protective clothing to avoid eye, skin or mucous membrane contact with the reagent. In case of the reagent coming into contact with a sensitive area, wash the area with large amounts of water. Microbial contamination of the reagent must be avoided, since otherwise non-specific staining might appear. ProClin is used for stabilisation. A Material safety data sheet (MSDS) is available upon request.
The Plus AP Polymer anti-Rabbit is designed for the qualitative detection of antigens in fixed paraffin-embedded tissue sections, in frozen tissue sections, and in cytological samples. It is developed for use in combination with monoand polyclonal primary antibodies and sera obtained from rabbits. The reagent can be used for examining tissues fixed in different solutions, e.g. formalin (neutrally buffered), B5, Bouin, ethanol, or HOPE.
The purpose of the immunohistochemical staining is to make tissue and cell antigens visible. The Plus AP Polymer anti-Rabbit is a highly sensitive detection reagent intended for use in immunohistochemistry and immunocytochemistry. The enzyme polymer in this kit consists of several molecules of secondary antibodies covalently bound to several molecules of alkaline phosphatase (AP). Visualisation occurs via an enzyme-substrate reaction in the presence of a colourising reagent which permits microscopical analysis. The reagent is suitable for the detection of mono- and polyclonal primary antibodies and sera obtained from rabbits. In contrast to other detection techniques, which often use the streptavidin-biotin system the Plus AP Polymer anti-Rabbit avoids the problem of background staining caused by endogenous biotin in the tissue.
Principle of method:
Paraffin-embedded tissue sections are first deparaffinised and rehydrated. Background staining caused by unspecific binding of the primary antibody or the secondary antibody in the AP polymer is minimized by incubation with a protein blocking solution (Blocking Solution). This step can be omitted if the primary antibodies are diluted in an appropriate buffer. The next step is incubation with the specific primary antibody. After washing, the AP-polymer is applied and incubated. Any excess of unbound AP-polymer is thoroughly washed away after incubation. The addition of the chromogenic substrate starts the enzymatic reaction of the alkaline phosphatase which leads to colour precipitation where the primary antibody is bound. The colour can be observed with a light microscope. The chromogen used determines the colour. The chromogen Permanent AP Red leads to the formation of a magentared product of reaction at the place of the target antigen.
Reagents provided:
6 ml AP-Polymer anti-Rabbit (Ready-to-use) Substrate systems recommended: Permanent AP Red kit. Materials required but not supplied: Positive und negative control tissue Xylene or suitable substitutes Ethanol, distilled H2O Reagents for enzyme digestion or heat pre-treatment Wash buffer PBS or TBS PAP Pen Primary antibody (user-defined) Primary antibody diluent Negative control reagent Chromogenic substrate Counter stain solution Mounting medium Cover slips
Storage and handling:
The solutions should be stored at 2-8°C without further dilution. Please store the reagent in a dark place and do not freeze it. Under these conditions the solutions are stable up to the expiry date indicated on the label. They should not be used after the expiry date. A positive and a negative control have to be carried out in parallel to the test material. If you observe unusual staining or other deviations from the expected results which could possibly be caused by the kit reagents, please contact our technical support
Reagent preparation:
Reagents should be at room temperature when used. ? Deparaffinise and rehydrate paraffin-embedded tissue sections. ? Pre-treatment (optional) with HIER (Heat Induced Epitope Retrieval) or enzymatic digestion. ? Tissue sections have to be completely covered with the different reagents in order to avoid drying out
Procedure:
1. Blocking Solution (protein block, this step is optional) 5 min. 2. Washing with wash buffer 1 x 2 min. 3. Primary antibody (optimally diluted) or negative control reagent 30-60 min. 4. Washing with wash buffer 3 x 5 min. 5. AP-polymer anti Rabbit 30 min. 6. Washing with wash buffer 3 x 2 min. 7. Permanent AP Red 5-15 min. (Controlling the colour intensity via light microscope is recommended.) 8. Stopping the reaction with distilled H2O when the desired colour intensity is attained 9. Counterstaining and blueing 10. Mounting: permanent or aqueous with Permanent AP Red
Expected results:
During the reaction of the substrate with alkaline phosphatase in the presence of a chromogen, a coloured precipitate is formed at the location of the bound primary antibody. This reaction only takes place if the target antigen is existent in the tissue. The chromogen used determines the colour of the precipitate. The analysis is carried out using a light microscope.
Trouble shooting:
If you observe unusual staining or other deviations from the expected results which could possibly be caused by the reagents, please read these instructions carefully, contact our technical support . No staining on an actually positive control slide: 1. Reagents were not used in the proper order. 2. Chromogenic substrate solution was too old. 3. Bleaching because chromogen and mounting medium are incompatible. 4. The antigen/epitope in the tissue was insufficiently accessible to the primary antibody. Try a pre-treatment such as heat pre-treatment or enzyme digestion. If you used a pre-treatment it should be extended. 5. Primary antibody not from rabbit, but from a different species. 6. The antigen/epitope was not stable in the fixation and/or pre-treatment procedure used. Try another fixation or pre-treatment. Weak staining: 1. Inadequate fixation or overfixation. 2. Incomplete deparaffinisation. 3. The antigen/epitope in the tissue was insufficiently accessible to the primary antibody. If you used heat pre-treatment or enzyme digestion it should be extended. 4. Excessive incubation with Blocking Solution or insufficient washing after this step. 5. Too much wash buffer remains on the slides after washing, diluting the reagents applied in the next step. 6. If you are using PBS-based wash buffer: the activity of alkaline phosphatase in the reagents is blocked if too much wash buffer remains on the slides. 7. Incubation times were too short or primary antibody concentration too low. 8. Chromogenic substrate solution was too old. Non-specific background staining or overstaining: 1. Incomplete deparaffinisation. 2. Excessive tissue adhesive on slides. 3. Insufficient washing especially after the incubation with the enzyme polymer or the chromogenic substrate solution. These washings are critical. 4. Tissue was allowed to (partially) dry out with reagents on. 5. Unspecific binding of the primary antibody. Please use a Blocking Solution or dilute the primary antibody in appropriate diluents. 6. Incubation time of the primary antibody was too long or primary antibody concentration too high. 7. Incubation time of the chromogenic substrate solution was too long or reaction temperature too high (e.g. if temperature in the laboratory is high).
Quality Control:
We recommend carrying out a positive and a negative control with every staining run. The positive control permits the validation of appropriate processing of the sample. If the negative control has a positive result, this points to unspecific staining.
Performance characteristics:
Studies have been conducted to evaluate the performance of the kit reagents. The product has been found to be suitable for the intended use
Limitations of procedure:
Immunohistochemistry is a complex method in which histological as well as immunological detection methods are combined. Tissue processing and handling prior to immunostaining, for example variations in fixation and embedding or the inherent nature of the tissue can cause inconsistent results (Nadji and Morales, 1983). Inadequate counterstaining and mounting can influence the interpretation of the results. The colour intensity of the reaction product can decrease with time, especially when exposed to light. Sanbio guarantees that the product will meet all requirements described from its shipping date until its expiry date, as long as the product is correctly stored and utilized. No additional guarantees can be given. Under no circumstances shall Sanbio be liable for any damages arising out of the use of the reagent provided
Precautions:
Use by qualified personnel only. Wear protective clothing to avoid eye, skin or mucous membrane contact with the reagents. In case of the reagent coming into contact with a sensitive area, wash the area with large amounts of water. Microbial contamination of the reagent must be avoided, since otherwise non-specific staining might appear. ProClin 950 is used for stabilisation. A Material safety data sheet (MSDS) is available upon request.
Noggin, Human, Purified Recombinant Protein, suitable for Cell Culture.
Background Info:
Noggin is a glycoprotein predominantly expressed by the dorsal mesoderm during embryogenesis and is secreted as a covalently linked homodimer. Noggin is essential for cartilage morphogenesis and joint formation. It is an inhibitor of bone morphogenic proteins (BMP) signaling which is required for growth and patterning of the neural tube and somite. Defects in Noggin are a cause of symphalangism proximal syndrome (SYM1), of multiple synostoses syndrome 1 (SYNS1), of the tarsal-carpal coalition syndrome (TCC), of stapes ankylosis with broad thumb and toes, and of brachydactyly type B2 (BDB2).
Product Type:
Protein
Format:
The Noggin product consists of 5-25% carbohydrate by weight.
Applications:
Cell Culture
Alternative Names:
Nog;
Biosensis Brand:
Biosensis®
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Storage:
Lyophilized products should be stored at 2-8°C. Following reconstitution, short-term storage at 2-8°C is recommended and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Purification:
>95% as determined by SDS-PAGE and visualized by silver stain.
The blocking solution Peroxide Block is intended for inhibition of endogenous peroxidase activity in tissue sections. It is primarily intended to be used in immunohistochemistry on formalin-fixed paraffin-embedded samples if using a detection system with horse radish peroxidase.
Endogenous peroxidase activity in the tissue can result in unspecific background staining in immunohistochemical staining procedures using horse radish peroxidase (HRP) as detection enzyme. This effect can be eliminated when tissue sections are incubated with Peroxide Block prior to immunohistochemical staining. Hydrogen peroxide in the solution blocks the activity of endogenous peroxidase.
Principle of method:
Peroxide Block is applied onto tissue sections to reduce non-specific staining due to endogenous peroxidase activity in immunohistochemistry. The step is carried out before incubation with primary antibody but after dewaxing and rehydration. If a heat induced epitope retrieval (HIER) or enzymatic digestion is necessary for immunohistochemical detection it is of no importance if the Peroxide Block is used before or after this step. In some cases it has been shown, that blocking of endogenous peroxidase before the epitope retrieval leads to better results.
Reagents provided:
100 ml Peroxide Block (ready-to-use)
Storage and handling:
The solution should be stored at 2-8°C without furt her dilution. Please store the reagent in a dark place and do not freeze it. Avoid exposure to strong light. Under these conditions the solution is stable up to the expiry date indicated on the label. Do not use product after the expiry date. A positive and a negative control have to be carried out in parallel to the test material. If you observe unusual staining or other deviations from the expected results which could possibly be caused by this reagent, please contact our technical support.
Procedure:
1. Apply Peroxide Block for 10 minutes at room temperature. The section should be covered completely. 2. Rinse with wash buffer. 3. Proceed with next steps for immunohistochemical staining as usual starting with protein blocking or the primary antibody.
Expected results:
During the reaction of the substrate with horse radish peroxidase or alkaline phosphatase in the presence of a chromogen, a coloured precipitate is formed at the location of the bound primary antibody. This reaction only takes place if the target antigen is existent in the tissue. The chromogen used determines the colour of the precipitate. The analysis is carried out using a light microscope
Trouble shooting:
If you observe unusual staining or other deviations from the expected results please read these instructions carefully, contact our technical support. Also refer to the instructions of the detection systems containing Peroxide Block for guidance on general troubleshooting
Quality Control:
We recommend carrying out a positive and a negative control with every staining run. The positive control permits the validation of appropriate processing of the sample. If the negative control has a positive result, this points to unspecific binding. Please refer to the instructions of the detection system for guidance on general quality control procedures.
Performance characteristics:
Studies have been conducted to evaluate the performance of the kit reagents. The product has been found to be suitable for the intended use
Limitations of procedure:
Immunohistochemistry is a complex technique involving both histological and immunological detection methods. It requires a highly trained histotechnologist. Tissue processing and handling prior to immunostaining, for example variations in fixation and embedding or the inherent nature of the tissue can cause inconsistent results (Nadji and Morales, 1983). Inadequate counterstaining and mounting can influence the interpretation of the results. Sanbio guarantees that the product will meet all requirements described from its shipping date until its expiry date, as long as the product is correctly stored and utilized. No additional guarantees can be given. Under no circumstances shall Sanbio be liable for any damages arising out of the use of the reagent provided.
Precautions:
Use by qualified personnel only. Wear protective clothing to avoid contact of reagent or specimen with eye, skin or mucous membrane. In case of the reagent or specimen coming into contact with a sensitive area, wash the area with large amounts of water. Microbial contamination of the reagent must be avoided, since otherwise non-specific staining may occur. A material safety data sheet (MSDS) is available upon request
Trypsin Pretreatment Kit consists of 2 reagents for the preparation of a trypsin solution used for enzymatic epitope retrieval on formalin-fixed tissue sections on slides. This procedure (sometimes called PIER, Protease Induced Epitope Retrieval) is primarily used in immunohistochemical staining procedures. Trypsin Pretreatment Kit is for research use only.
Immunohistochemical staining procedures consist of sequential incubation steps with blocking solutions, antibodies and secondary reagents, enzymes and chromogenic substrates carried out on tissue sections. These tissue sections are mostly prepared out of formalin-fixed paraffin-embedded tissue blocks. Cellular structures are very effectively stabilised by formalin fixation which results in optimal morphological preservation of the sample. On the other hand the formalin fixation leads to strong cross-links between proteins. This means that epitopes of antigens are being masked and often are no longer accessible for primary antibodies. In order to enable primary antibodies to bind to antigens the epitopes have to be recovered. Enzymatic digestion with proteolytic enzymes (PIER) restores structures of the epitopes making them more accessible to specific antibodies. Heat induced epitope retrieval (HIER) in buffer solutions of different compositions and pH-values is another way of recovering epitopes. The primary antibody used determines the appropriate method.
Principle of method:
The components of this Trypsin Pretreatment Kit allow for the preparation of a buffered trypsin solution for enzymatic epitope retrieval.
Reagents provided:
30 ml Trypsin Solution 125 ml Trypsin Buffer
Storage and handling:
The solutions should be stored at 2-8°C. Please sto re the reagents in a dark place and do not freeze them. Under these conditions the solutions are stable up to the expiry date indicated on the label. Do not use product after the expiry date. A positive and a negative control have to be carried out in parallel to the test material. If you observe unusual staining or other deviations from the expected results which could possibly be caused by this reagent, please contact our technical support.
Reagent preparation:
Mix 1 part Trypsin Solution with 3 parts Trypsin Buffer. The activity of the resulting trypsin solution can be adjusted by variation of the mixing ratio. Mix the two components in the ratio 1:1 when strong epitope retrieval is desired. The working solution is stable for at least one week if stored at 2-8°C.
Procedure:
Trypsin Pretreatment Kit is suitable for enzymatic epitope retrieval carried out after the dewaxing and rehydration of the sections. 1. Cover deparaffinised and rehydrated tissue sections with trypsin working solution. 2. Incubate for 10 - 20 minutes at 37°C. 3. Rinse carefully (3 x) with wash buffer. 4. Proceed with immunohistological staining as usual.
Expected results:
During the reaction of the substrate with horse radish peroxidase or alkaline phosphatase in the presence of a chromogen, a coloured precipitate is formed at the location of the bound primary antibody. This reaction only takes place if the target antigen is existent in the tissue. The chromogen used determines the colour of the precipitate. The analysis is carried out using a light microscope.
Trouble shooting:
If you observe unusual staining or other deviations from the expected results please read these instructions carefully, or contact our technical support. Also refer to the instructions of the detection systems for guidance on general troubleshooting.
Quality Control:
We recommend carrying out a positive and a negative control with every staining run. The positive control permits the validation of appropriate processing of the sample. If the negative control has a positive result, this points to unspecific staining. Please refer to the instructions of the detection system for guidance on general quality control procedures.
Performance characteristics:
Studies have been conducted to evaluate the performance of the kit reagents. The product has been found to be suitable for the intended use
Limitations of procedure:
Immunohistochemistry is a complex technique involving both histological and immunological detection methods. It requires a highly trained histotechnologist. Tissue processing and handling prior to immunostaining, for example variations in fixation and embedding or the inherent nature of the tissue can cause inconsistent results (Nadji and Morales, 1983). Inadequate counterstaining and mounting can influence the interpretation of the results. Sanbio guarantees that the product will meet all requirements described from its shipping date until its expiry date, as long as the product is correctly stored and utilized. No additional guarantees can be given. Under no circumstances shall Sanbio be liable for any damages arising out of the use of the reagent provided.
Precautions:
Use by qualified personnel only. Wear protective clothing to avoid contact of reagent and specimen with eye, skin or mucous membranes. In case of reagent or specimen coming into contact with a sensitive area, wash area with large amounts of water. A material safety data sheet (MSDS) is available upon request.
NT3 is a member of the neurotrophin family, that controls survival and differentiation of visceral and proprioceptive sensory neurons. NT3 is closely related to both NGF and BDNF. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NT3-deficient mice generated by gene targeting display sevvere movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Chicken,Human,Rat
Immunogen:
A synthetic peptide (YAEHKSHRGEY) as part of human (aa: 139-149), mouse and rat NT3 protein conjugated to BSA has been used as the immunogen.
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
IgG
Application Details:
IHC, ELISA, WB, inhibition of biological activity. A concentration of 1-10 µg/mL is recommended for IHC, ELISA, WB and inhibition of biological activity in vitro; 2-10 µg/mL (ED50) for in vivo use. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
A cross reactivity of less than 0.1% to mouse NGF, recombinant human BDNF and NT4/5 has been shown by dot blot. This antiserum is known to react with rat, chicken and human NT3.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
A synthetic peptide (C-ELLDEDQKVRPNEE) as a part of human BDNF precursor protein (aa: 69-82) conjugated to KLH has been used as the immunogen.
Applications:
IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
IHC, WB. 1-5 µg/mL is recommended for both applications, Flow Cytometry (2?g/10^6 cells). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Le Blanc J et al. (2020) Platelets Selectively Regulate the Release of BDNF, But Not That of Its Precursor Protein, proBDNF. Front Immunol. 11:575607 Application: Human, WB. Macias M et al. (2007) Locomotor exercise alters expression of pro-brain-derived neurotrophic factor, brain-derived neurotrophic factor and its receptor TrkB in the spinal cord of adult rats. Eur J Neurosci. 25(8):2425-44 Application: Rat
Specificity:
Used in western blot, this antiserum detects a 35 kDa band corresponding to the molecular weight of proBDNF. No cross reactivity with other proneurotrophins was detected. This antibody is known to react with human, mouse and rat proBDNF and also expected to recognise other mammalian proBDNF.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Rabbit IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rabbit IgG · light chains on all rabbit immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rabbit serum proteins · serum proteins from bovine, goat, human, mouse, or rat · IgG from bovine, goat, human, mouse or rat
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Donkey anti-Rabbit IgG (H&L), F(ab)'2 Fragment, min. cross-reactivity to Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat or Sheep is a secondary antibody which binds to rabbit IgG (H&L), F(ab)'2 Fragment in immunological assays.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified donkey IgG.
Special application note:
Antibody purity is > 90% based on SDS-PAGE Small amounts of intact IgG may be present.Affinity purified using solid phase Rabbit IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on Rabbit IgG • light chains on all Rabbit immunoglobulins .Based on IEP, no reactivity is observed to: • non-immunoglobulin Rabbit serum immunoglobulins • IgG from Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat or Sheep.
For reconstitution add 1,1 ml of sterile water,Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Mouse IgM.Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.1% (v/v) Kathon CG is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on mouse IgM .Based on IEP, no reactivity is observed to: • non-immunoglobulin mouse serum proteins • light chains on all mouse immunoglobulins .
Donkey anti-Mouse IgG (H&L), F(ab)'2 Fragment, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep IgG is a secondary antibody which binds to mouse IgG (H&L), F(ab)'2 Fragment in immunological assays.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified donkey IgG.
Special application note:
Antibody purity is > 90% based on SDS-PAGE Small amounts of intact IgG may be present.Affinity purified using solid phase Mouse IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on mouse IgG • light chains on all mouse immunoglobulins .Based on IEP, no reactivity is observed to: • non-immunoglobulin mouse serum immunoglobulins • IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep .
Affinity purified using solid phase Human IgA + IgG + IgM
Purity:
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified human IgG + IgA + IgM, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy chains on human IgA + IgG + IgM · light chains on all human immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin human serum immunoglobulins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Chicken
Immunogen:
Purified Rabbit IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rabbit IgG · light chains on all rabbit immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rabbit serum proteins · IgG from human or mouse
Country Of Origin:
Chicken Ig fraction was prepared using serum from healthy hens of US origin.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Mouse IgG, whole molecule
Buffer:
PBS, 1% BSA & 0.1% proclin 150
Preservative:
0.1% (v/v) Kathon CG
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum proteins · bovine, goat, human, rabbit or rat IgG
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Chicken
Immunogen:
Purified Mouse IgG, whole molecule
Buffer:
PBS, 1% BSA & 0.1% proclin 150
Preservative:
0.1% (v/v) Kathon CG
Reconstitution:
Rehydrate with 0.55 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum immunoglobulins · IgG/serum from human or rabbit
Country Of Origin:
Chicken Ig fraction was prepared using serum from healthy hens of US origin.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Mouse IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum proteins · bovine, goat, human, rabbit or rat IgG
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Host:
Goat
Immunogen:
Purified Human IgG, Fc fragment
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.8, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on human IgG
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin human serum immunoglobulins · light chains on all human immunoglobulins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Donkey
Immunogen:
Purified Goat IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.8, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on goat IgG · light chains on all goat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin goat serum immunoglobulins · IgG from human, mouse, rabbit or rat
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on goat IgG · light chains on all goat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin goat serum immunoglobulins · IgG from human, mouse, rabbit or rat
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Host:
Goat
Immunogen:
Purified Human IgG, whole molecule
Buffer:
PBS, 1% BSA & 0.1% proclin 150
Preservative:
0.1% (v/v) Kathon CG
Reconstitution:
Rehydrate with 0.55 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on human IgG · light chains on all human immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin human serum immunoglobulins · serum proteins from bovine, mouse or rabbit
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Chicken
Immunogen:
Purified Mouse IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum immunoglobulins · IgG/serum from human or rabbit
Country Of Origin:
Chicken Ig fraction was prepared using serum from healthy hens of US origin.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Human IgG, Fc fragment
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on human IgG
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin human serum immunoglobulins · light chains on all human immunoglobulins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified using solid phase Human IgA + IgG + IgM
Purity:
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Host:
Goat
Immunogen:
Purified human IgG + IgA + IgM, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy chains on human IgA + IgG + IgM · light chains on all human immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin human serum immunoglobulins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Chicken
Immunogen:
Purified Goat IgG, whole molecule
Buffer:
PBS, 1% BSA & 0.1% proclin 150
Preservative:
0.1% (v/v) Kathon CG
Reconstitution:
Rehydrate with 0.55 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on goat IgG · light chains on all goat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin goat serum immunoglobulins · IgG/serum proteins from human, mouse or rabbit
Country Of Origin:
Chicken Ig fraction was prepared using serum from healthy hens of US origin.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Chicken
Immunogen:
Purified Rat IgG, whole molecule
Buffer:
PBS, 1% BSA & 0.1% proclin 150
Preservative:
0.1% (v/v) Kathon CG
Reconstitution:
Rehydrate with 0.55 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rat IgG · light chains on all rat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rat serum proteins · serum proteins from human or rabbit · IgG from human or rabbit
Country Of Origin:
Chicken Ig fraction was prepared using serum from healthy hens of US origin.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Chicken
Immunogen:
Purified Rat IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rat IgG · light chains on all rat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rat serum proteins · serum proteins from human or rabbit · IgG from human or rabbit
Country Of Origin:
Chicken Ig fraction was prepared using serum from healthy hens of US origin.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Chicken
Immunogen:
Purified Goat IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on goat IgG · light chains on all goat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin goat serum immunoglobulins · IgG/serum proteins from human, mouse or rabbit
Country Of Origin:
Chicken Ig fraction was prepared using serum from healthy hens of US origin.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Chicken
Immunogen:
Purified Rabbit IgG, whole molecule
Buffer:
PBS, 1% BSA & 0.1% proclin 150
Preservative:
0.1% (v/v) Kathon CG
Reconstitution:
Rehydrate with 0.55 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rabbit IgG · light chains on all rabbit immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rabbit serum proteins · IgG from human or mouse
Country Of Origin:
Chicken Ig fraction was prepared using serum from healthy hens of US origin.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified using solid phase Mouse IgG + IgM
Purity:
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Mouse IgG + IgM, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy chains on mouse IgG + IgM · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum proteins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Host:
Goat
Immunogen:
Purified Mouse IgG, whole molecule
Buffer:
PBS, 1% BSA & 0.1% proclin 150
Preservative:
0.1% (v/v) Kathon CG
Reconstitution:
Rehydrate with 0.55 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum immunoglobulins · human IgG or serum proteins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Host:
Goat
Immunogen:
Purified Rabbit IgG, whole molecule
Buffer:
PBS, 1% BSA & 0.1% proclin 150
Preservative:
0.1% (v/v) Kathon CG
Reconstitution:
Rehydrate with 0.55 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rabbit IgG · light chains on all rabbit immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rabbit serum proteins · serum proteins from bovine, human, or mouse · IgG from human or mouse
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Rabbit
Immunogen:
Purified Hamster IgG, whole molecule
Buffer:
PBS, 1% BSA & 0.1% proclin 150
Preservative:
0.1% (v/v) Kathon CG
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on hamster IgG · light chains on all hamster immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin hamster serum immunoglobulins · human serum proteins
Country Of Origin:
Rabbit serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Rat IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rat IgG · light chains on all rat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rat serum proteins · IgG and Serum from human, mouse or rabbit
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Rabbit
Immunogen:
Purified Rat IgG, whole molecule
Buffer:
PBS, 1% BSA & 0.1% proclin 150
Preservative:
0.1% (v/v) Kathon CG
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rat IgG · light chains on all rat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rat serum proteins · human serum proteins
Country Of Origin:
Rabbit serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Rabbit
Immunogen:
Purified Rat IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rat IgG · light chains on all rat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rat serum proteins · human serum proteins
Country Of Origin:
Rabbit serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Human IgA, (α chain)
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (α) chains on human IgA
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin human serum immunoglobulins · light chains on all human immunoglobulins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Mouse IgM, µ chain
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (µ) chains on mouse IgM
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum proteins · light chains on all mouse immunoglobulins · human IgG or serum proteins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Rabbit
Immunogen:
Purified goat IgG, whole molecule
Buffer:
30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on goat IgG · light chains on all goat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin goat serum immunoglobulins · IgG from human, mouse or rat
Country Of Origin:
Rabbit serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on goat IgG
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin goat serum immunoglobulins · light chains on all goat immunoglobulins · goat IgG, F(ab)2 fragment · human serum proteins
Country Of Origin:
Rabbit serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
PHR1 (Phosphate starvation response 1) is nuclear localized and involved in transcription regulation.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Lyophilized antibody can be stored at -20 °C for up to 3 years. Re-constituted antibody can be stored at 4°Cfor several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Hordeum vulgare (recombinant PHR1)
Expected Species:
Aegilops tauschii, Brachypodium distachyon, Dichanthelium oligosanthes, Panicum hallii Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from Hordeum vulgare PHR1, UniProt: F4Y5E9
ABI4 (Abscisic acid insensitive 4) is a transcription factor involved in the regulation of gene expression by stress factors. Confers sensitivity to abscisic acid (ABA) and regulates the ABA signaling pathway during seed germination upon nitrate-mediated lateral root inhibition, in hexokinase-dependent sugar responses (including feed-back regulation of photosynthesis and mobilization of storage lipid during germination), and in response to osmotic stress mediated by NaCl, KCl or mannitol. Plays a role in sucrose sensing or signaling, especially at low fluence far red light. ABI4 gene is expressed most abundantly in developing sliliques and to a lesser degree in seedlings. Alternative names: ABSCISIC ACID INSENSITIVE 4, ABI4, GLUCOSE INSENSITIVE 6, GIN6, IMPAIRED SUCROSE INDUCTION 3, ISI3, SALOBRENO 5,SAN5, SUCROSE UNCOUPLED 6, SUN6, SUGAR INSENSITIVE 5, SIS5, ERF ABI4, Ethylene-responsive transcription factor ABI4, ERF052, At2g40220, T7M7_16, T7M7.16.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana (recombinant ABI4)
Expected Species:
Arachis duranensis, Brassica oleracea, Camelia sativa, Carica papaya, Cicer arietinum, Cinnamomum micranthum f. kanehirae, Citrus clementina, Coffea arabica, Cucurbita maxima, Cucurbita pepo, Eutrema salsugineum, Fragaria vesca, Glycine soja, Lupinus angustifolius, Malus domestica, Medicago truncatula, Morus notabilis, Nicotiana attenuata, Nicotiana tabacum, Papaver somniferum, Pyrus x bretschneideri, Sesamum indicum, Tarenaya hassleriana, Theobroma cacao, Trifolium pratenseSpecies of your interest not listed? Contact us
ABP1 is an auxin receptor which regulates polar auxin transport. It is involved in the shade avoidance responsse, controls cell division and elongation, controls the size of a root meristem and madiates auxin responsivness. ABP1 promotes endocytosis via clathrin recruitment and restricts PIN internalization upon auxin binding via inhibition of clathrin-mediated endocytosis.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Streptavidin HRP conjugate is designed to be used in the detection of biotinylated primary antibodies.This product is ELISA grade.
Product Type:
Antibody
Storage Temp:
Store freeze-dried (lyophilized) powder at 2-8°C. Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilution daily prior to use and then discard.Shelf life of this product is 1 year from date of receipt.
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Buffer composition: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.1 % Kathon CG (v/v) is included as preservative. Streptavidin was overexpressed in bacterial culture, purified and conjugated to horse radish peroxidase (HRP).
NDH (NAD(P)H-quinone oxidoreductase subunit 5, chloroplastic) is involved in the transfer of electrons from NAD(P)H:plastoquinone, via FMN and iron-sulfur centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. Alternative names: NAD(P)H-quinone oxidoreductase subunit 5, chloroplastic, NADH-plastoquinone oxidoreductase subunit 5.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
The antibody may be stored at -20 °C for one year in its original formulation. Additionally, antibody may be stored at 2°Cto 8°Cfor up to 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles of the diluted antibody.
Host Animal:
Rabbit
Species Reactivity:
Oryza sativa
Immunogen:
KLH-conjugated synthetic peptide derived from Oryza sativa OsChl78 UniProt: P0C328
DCL5 (Zea mays Dicer-like 102) may be involved in precise slicing in a range of monocots.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from Zea mays DCL5 protein sequence UniProt: A0A1D6KSK2
CGLD27 (CONSERVED IN THE GREEN LINEAGE AND DIATOMS 27) is required for growth in low iron conditions. Localized in chloroplasts. Mostly expressed in seeds, leaves and flowers and at a lower extent in roots.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana (recombinant CGLD27)
Expected Species:
Chlamydomonas reinhardtiSpecies of your interest not listed? Contact us
Immunogen:
thioredoxin fusion of CGLD27 of Arabidopsis thaliana, UniProt Q9FN15-1 TAIR At5g67370
Gene At5g11840 is encoding a chloroplast localized protein, called also YCF36.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
higher plants, for specific species Species of your interest not listed? please inquire
Immunogen:
Full length, recombiant protein of Arabidopsis thaliana, UniProt: B6IDH6-1, TAIR:At5g11840
VP1 | Viviparous 1 (ABI3) is a transcription factor, which participates in abscisic acid-regulated gene expression during seed development. Alternative names: B3 domain-containing transcription factor ABI3, Protein ABSCISIC ACID-INSENSITIVE 3.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana (recombinant ABI3)
Expected Species:
Cajanus cajan, Cuscuta campestris, Juglans regia, Linum usitatissimum, Glycine max, Jatropha curcas, Populus trichocarpa, Pisum sativum, Sorghum bicolor, Ricinis communis, Zea mays, Zostera marina, Vitis vinifera Species of your interest not listed? Contact us
ELF4 (Early flowering 4) is the component of the central CCA1/LHY-TOC1 negative feedback loop in the circadian clock that promotes clock accuracy and is required for sustained rhythms in the absence of daily light/dark cycles. Increases ELF3 nuclear distribution and localization in nuclear bodies. ELF4 is necessary for light-induced expression of both CCA1 and LHY and mediates both entrainment to an environmental cycle and circadian rhythm sustainability under constant conditions. Controls flowering time. Alternative name: Protein ARRHYTHMIC 44.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Lyophilized antibody can be stored at -20 °C for up to several years. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide derived from N-terminal of Arabidopsis thaliana ELF4, UniProt: O04211, TAIR: AT2G40080
ASY1 (Asynapsis 1) is a protein required for normal meiosis in male and female gametophytes, which plays a crucial role in coordinating the activity of DMC1. Alternative names: Meiosis-specific protein ASY1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Hordeum vulgare
Expected Species:
Arabidopsis thaliana, Hordeum vulgare, Oryza sativa, Triticum aestivum, Zea maysSpecies of your interest not listed? Contact us
Immunogen:
Recombinant ASY1 protein from Hordeum vulgare, UniProt: A0A8I6YI54
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Donkey
Immunogen:
Purified Rabbit IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rabbit IgG · light chains on all rabbit immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rabbit serum proteins · IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat or sheep
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Host:
Goat
Immunogen:
Purified Human IgG, Fc
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on human IgG
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin human serum immunoglobulins · light chains on all human immunoglobulins · human IgA or IgM · serum proteins from bovine, mouse, or rabbit
Country Of Origin:
Normal Goat Serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.1% (v/v) Kathon CG
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.
Specificity:
This antibody reacts with Biotin
Cross Reactivity:
This antibody does not react with free BSA or free KLH
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Rabbit anti-Goat IgG (H&L), DyLight 488 conjugated, min. cross-reactivity to human, mouse, rat lgG is a secondary antibody conjugated to DyLight 488, which binds to Goat IgG (H&L) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Goat IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries
For reconstitution add 1,1 ml of sterile water,Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Goat IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on goat IgG • light chains on all goat immunoglobulins .Based on IEP, no reactivity is observed to: • non-immunoglobulin goat serum immunoglobulins • IgG from human, mouse or rat .
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.05% (w/v) sodium azide.
Special application note:
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Bovine IgG (H&L).Antibodi is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2.0.05% (w/v) Sodium Azide is added as preservative.Based on IEP, this antibody reacts with: heavy (γ) chains on bovine IgG light chains on all bovine immunoglobulins. Based on IEP, no reactivity is observed to: non-immunoglobulin bovine serum immunoglobulins.
For reconstitution add 1,1 ml of sterile water,Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Mouse IgM.Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on mouse IgM • light chains on all mouse immunoglobulins .Based on IEP, no reactivity is observed to: • non-immunoglobulin mouse serum proteins .
(6-4)DNA photolyase; Involved in repair of UV radiation-induced DNA damage. Catalyzes the photoreactivation of pyrimidine [6-4] pyrimidone photoproduct (6-4 products). Binds specifically to DNA containing 6-4 products and repairs these lesions in a visible light- dependent manner. Not required for repair of cyclobutane pyrimidine dimer (CPD).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana UVR3-GFP
Immunogen:
KLH-conjugated peptide derived from Arabidopsis thaliana UVR3 protein sequence, UniProt: O48652-1, TAIR: AT3G15620
No confirmed exceptions from predicted reactivity are currently known
Selected references:
To be added when available, antibody available in September 2022.
Special application note:
Reactivity of this antibbody on endogenous material remains to be determined. UVR3 is a low abundance protein, therefore use of specific cellular fraction or concentration through TCA/acetone precipitation is recommended.
For reconstitution add 1,1 ml of sterile water,Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Mouse IgM.Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on mouse IgM .Based on IEP, no reactivity is observed to: • non-immunoglobulin mouse serum proteins • light chains on all mouse immunoglobulins .
ICS1 | Isochorismate synthase 1 (chloroplastic) is involved in stress response and defence response to bacterium, fungus, leaf abscission, cold. Alternative names: AtICS1, EDS16, Enhanced disease susceptibility to erysiphe orontii 16, Salicyic acid induction deficient 2, SID2.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana (recombinant ICS1)
Expected Species:
please inquire
Immunogen:
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana ICS1 protein sequence, UniProt: Q9S7H8, TAIR: At1g74710.The peptide is perfectly conserved in ICS1-1 and ICS1-2 isoforms of Arabidopsis thaliana.
ICS1 is present in too low levels to allow detection in control conditions. Antibody is recognzing ICS1 in transgenic lines which have ICS1 under 35S promotor and is recognizing well YFP-tagged ICS1.
Application Details:
1 : 1000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
62 | 55 kDa
Not reactive in:
Solanum lycopersicum, Zea mays
Selected references:
To be added when available, antibody released in January 2021
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signalling systems.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Oryza sativa
Expected Species:
Arabidopsis thaliana
Immunogen:
recombinat protein of Oryza sativa expressed in E.coli
Goat anti-Mouse IgG + IgM,, DyLight 633 conjugated, min w/human IgG or serum proteins is a secondary antibody conjugated to DyLight 633, which binds to mouse IgG + IgM in immunological assays. DyLight 633 has Amax = 638 nm, Emax = 658 nm. DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
For reconstitution add 1,1 ml of sterile water,Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Mouse IgG + IgM.Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: • heavy chains on mouse IgG + IgM • light chains on all mouse immunoglobulins .Based on IEP, no reactivity is observed to: • non-immunoglobulin mouse serum proteins • human IgG or serum proteins .
Store lyophilized material at 2-8 °C. For long term storage after reconstitution, prepare small aliquots and store at -20 °C. For storage at 2-8 C, add a preservative to prevent growth of bacteria.This product is used as a blocking reagent or control for
Suitable as a control or blocking reagent in immunoassays
Application Details:
To be determined by end user
Purity:
Purified serum, lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2.
Special application note:
Rehydrate with 11 ml of deionized water,(Product has been overfilled to ensure complete recovery,) Swirl gently and let stand for up to 2 hours at 18-25 C, Centrifuge reconstituted serum to remove any precipitates
Cytokinins (CK) belong to a class of plant growth substances (plant hormones) which promote cell deivision by means of local and long distance signalling.Zeatin is an adenine-type cytokinin synthesised in stems, leaves and roots.
Product Type:
Antibody
Storage Temp:
Aliquote and store at -20 °C to avoid freezing and thawing cycles.
Goat anti-Mouse IgG Fc, HRP conjugated, min. cross-reactivity to bovine, horse or human serum proteins is a secondary antibody conjugated to HRP, which binds to mouse IgG Fc in immunological assays.
For reconstitution add 1,1 ml of sterile water,Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Mouse IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.1% (v/v) Kathon CG is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on mouse IgG .Based on IEP, no reactivity is observed to: • non-immunoglobulin mouse serum proteins • light chains on all mouse immunoglobulins • mouse IgG, F(ab)'2 fragment • serum proteins from bovine, horse, or human .
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Host:
Goat
Immunogen:
Purified Mouse IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.8, 1 % (w/v) BSA, Protease/IgG free
Storage:
Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum immunoglobulins · serum proteins from bovine, horse, human rabbit or swine
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Donkey
Immunogen:
Purified Rabbit IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20°C.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rabbit IgG · light chains on all rabbit immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rabbit serum proteins · mouse IgG or serum proteins
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Trademark:
DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Mouse IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.8, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum immunoglobulins · serum proteins from bovine, horse, human, pig, or rabbit
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Host:
Goat
Immunogen:
Purified Mouse IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Reconstitution:
Rehydrate with 0.55 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum immunoglobulins · serum proteins from bovine, horse, human rabbit or swine
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Human IgG, Fc fragment
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on human IgG
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin human serum immunoglobulins · light chains on all human immunoglobulins · human IgG F(ab)2 fragment · mouse IgG1 · serum proteins from bovine, horse, mouse, or rabbit
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Mouse IgM, whole molecule
Buffer:
PBS, 1% BSA & 0.1% proclin 150
Preservative:
0.1% (v/v) Kathon CG
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.
Specificity:
Based on IEP, this antibody reacts with: · heavy (µ) chains on mouse IgM · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum proteins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
This antibody reacts with Biotin
Cross Reactivity:
This antibody does not react with free BSA or free KLH
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Host:
Goat
Immunogen:
Purified Rabbit IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rabbit IgG · light chains on all rabbit immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rabbit serum proteins · serum proteins from bovine, human, or mouse · IgG from human or mouse
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.8, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
This antibody reacts with Biotin
Cross Reactivity:
This antibody does not react with free BSA or free KLH
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on rat IgG · light chains on all rat immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin rat serum proteins · human serum proteins
Country Of Origin:
Rabbit serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Goat anti-mouse IgM, ( chain) affinity purified is a secondary antibody which binds to mouse IgM in immunological assay. Antibodies are affinity purified using solid phase mouse IgM.
The optimal working dilution should be determined by the investigator, Antibody is suitbale for: ELISA, ICC, IHC, WB
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the mu chain on mouse IgM based on immunoelectrophoresis.Minimum cross-reactivity is observed with the light chains based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis and and has limited reaction with purified mouse IgG based on ELISA.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 0.05 % (w/v) sodium azide as preservative. The purity > 95 % based on SD-PAGE.
Donkey anti-Rabbit IgG (H&L), DyLight 488 Conjugated, min. cross-reactivity to human IgG and serum proteins is a secondary antibody conjugated to DyLight 488, which binds to Rabbit IgG (H&L) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Rabbit IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG, light chains on all rabbit immunoglobulinsNo reactivity is observed to: non-immunoglobulin rabbit serum proteins, mouse IgG or serum proteins
No reactivity is observed to the light chains or non-immunoglobulin human serum proteins based on immunoelectrophoresis.No reactivity to bovine, mouse or rabbit serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of Sodium azide as preservative.Antibody concentration is 4.5 mg/ml.
Goat anti-rabbit IgG, DyLight 488 Conjugate is a secondary antibody conjugated to DyLight 488, which binds to all rabbit IgGs in immunological assays.DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based in immunoelectrophoresis, this antibody reacts with heavy chains on rabbit IgG and light chains on all rabbit immunoglobulins.No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis. Purity of this antibody is > 95% based on SDS-PAGE.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Selected references:
Kucko et al. (2022) The acceleration of yellow lupine flower abscission by jasmonates is accompanied by lipid-related events in abscission zone cells, Plant Science, Volume 316, 2022,111173, ISSN 0168-9452, https://doi.org/10.1016/j.plantsci.2021.111173. Namyslov et al. (2020). Exodermis and Endodermis Respond to Nutrient Deficiency in Nutrient-Specific and Localized Manner. Plants (Basel). 2020 Feb 6;9(2). pii: E201. doi: 10.3390/plants9020201. (immunolocalization) Fizesan et al. (2018). Responsiveness assessment of a 3D tetra-culture alveolar model exposed to diesel exhaust particulate matter. Toxicol In Vitro. 2018 Aug 3;53:67-79. doi: 10.1016/j.tiv.2018.07.019.Liu et al. (2016). Fold formation at the compartment boundary of Drosophila wing requires Yki signaling to suppress JNK dependent apoptosis. Sci Rep. 2016 Nov 29;6:38003. doi: 10.1038/srep38003.Wang et al. (2016). Complementary expression of optomotor-blind and the Iroquois complex promotes fold formation to separate wing notum and hinge territories. Dev Biol. 2016 Aug 1;416(1):225-34. doi: 10.1016/j.ydbio.2016.05.020. Epub 2016 May 19
Special application note:
Concentration: 1.0mg/mlConjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.DyLight 488 has a maximum absorbance at 493 nm; Emax = 518 nm.
Goat anti-rat IgG is a secondary antibody conjugated to HRP which binds to all rabbit IgGs in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store non-diluted antibody at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Rat IgG heavy and light chains (H&L) of all Rat immunoglobulins
Expected Species:
Rat IgG Heavy and Light chains (H&L) of all Rat immunoglobulins
Immunogen:
Purified Rat IgG, whole molecule
Applications:
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
No reactivity is observed to non-immunoglobulin rat serum, Antibody has been absorbed against mouse IgG and no reactivity is observed to human or mouse IgG based on immunoelectrophoresis
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative.
Donkey anti-Sheep IgG (H&L), DyLight 488 Conjugated is a secondary antibody conjugated to DyLight 488, which binds to Sheep IgG (H&L) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Sheep IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on sheep IgG, light chains on all sheep immunoglobulinsNo reactivity is observed to: non-immunoglobulin sheep serum proteins
Donkey anti-sheep IgG is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to all donkey immunoglobulins in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Donkey
Species Reactivity:
Sheep IgG heavy and light chains (H&L)
Expected Species:
Sheep IgG Heavy and Light chains (H&L)
Immunogen:
Purified sheep IgG, whole molecule
Applications:
ELISA (ELISA) , Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
No reactivity is observed to non-immunoglobulin sheep serum proteins based in immunoelectrophoresis.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
No confirmed exceptions from predicted reactivity are currently known
Special application note:
APL conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
Donkey anti-mouse IgG (H&L), affinity purified is a secondary antibody which binds to mouse IgG in immunological assay. Antibodies are affinity purified using solid phase mouse IgG.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 0.05 % (w/v) sodium azide as preservative.
Donkey anti-sheep IgG is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to all sheep immunoglobulins in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For storage at -20 °C after reconstitution dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Donkey
Species Reactivity:
Sheep IgG heavy chains and light chains on all sheep immunoglobulins
Immunogen:
Purified sheep IgG, whole molecule
Applications:
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily
Not reactive in:
non-immunoglobulin sheep serum proteins based in immunoelectrophoresis
Special application note:
Antibody concentration is 1 mg/ml and it has been purified by antigen-specific chromatographyHRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of ProClin 150 is used as preservative.For blocking: BSA and nonfat milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Donkey anti-Mouse IgG (H&L), DyLight 488 Conjugated is a secondary antibody conjugated to DyLight 488, which binds to Mouse IgG (H&L) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Mouse IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG, light chains on all mouse immunoglobulinsNo reactivity is observed to: non-immunoglobulin mouse serum proteins
Goat anti-human IgA heavy (alpha chain) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgA heavy, alpha chains in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Human IgA, heavy alpha chains
Expected Species:
Human IgA, Heavy alpha chains
Immunogen:
purified human IgA, alpha chain
Applications:
ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
No reactivity is observed to light chains or non-IgA human serum proteins based in immunoelectrophoresisno reactivity to bovine, mouse or rabbit serum proteins based on immunoelectrophoresis
No confirmed exceptions from predicted reactivity are currently known
Special application note:
AP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
Rabbit anti-goat IgG is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to all goat immunoglobulins in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Rabbit
Species Reactivity:
Goat IgG heavy and light chains on all goat immunoglobulins
Immunogen:
Purified goat IgG, whole molecule
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
For blocking BSA and non-fat milk is recommended to be replaced by other blocking reagents, like donkey serum or commercial formulations which are free from bovine IgG.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily
Not reactive in:
Non-immunoglobulin goat serum proteins based in immunoelectrophoresis
Selected references:
Sinclair et al. (2017) Etiolated Seedling Development Requires Repression of Photomorphogenesis by a Small Cell-Wall-Derived Dark Signal. Curr Biol. 2017 Nov 20;27(22):3403-3418.e7. doi: 10.1016/j.cub.2017.09.063.
Special application note:
Concentration: 1.0 mg/mlHRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free with 0.1 % (v/v) of ProClin 150 as preservative.
Goat anti-human IgA heavy (alpha chain) is a secondary antibody conjugated to biotin which binds to human IgA heavy alpha chains in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store non-diluted antibody at 2-8°C. Shelf life is 1 year from date of receipt.
No reactivity is observed to light chains or non-IgA human serum proteins based on immunoelectrophoresis.No reactivity to bovine, mouse, rabbit serum based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Biotin conjugate is supplied in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, Contains 0,05 % (w/v) sodium azide as preservative
Goat anti-chicken IgY is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to hen IgY immunoglobulin in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Chicken IgY heavy and light chains (H&L)
Expected Species:
Chicken IgY Heavy and Light chains (H&L)
Immunogen:
purified chicken IgY, whole molecule
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Concentration: 1.50 mg/ml (E 1% at 280 nm = 13.0)Antibody concentration is 1.14 mg/ml and it has been afinity purified on solid phase hen IgY (H&L)Antibody is affinity purified using solid phase Chicken IgG/Y.AP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative
Goat anti-rat IgG is a secondary antibody conjugated to ALP (alkaline phosphatase) which binds to all rabbit immunoglobulins in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Rat IgG heavy and light chains (H&L)
Expected Species:
Rat IgG Heavy and Light chains (H&L)
Immunogen:
Purified Rat IgG, whole molecule
Applications:
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
No reactivity is observed to non-immunoglobulin rat serum proteins based in immunoelectrophoresis, No reacticity to mouse and human serum proteins based on immunoelectrophoresis
No confirmed exceptions from predicted reactivity are currently known
Special application note:
ALP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
Rabbit anti-goat IgG (H&L), DyLight 488 Conjugated is a secondary antibody conjugated to DyLight 488, which binds to Goat IgG (H&L) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Goat IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified rabbit IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with:, heavy (γ) chains on goat IgG, light chains on all goat immunoglobulinsNo reactivity is observed to: non-immunoglobulin goat serum proteins, IgG from human, mouse, or rat
Goat anti-Chicken IgY (H&L), DyLight 488 Conjugated is a secondary antibody conjugated to DyLight 488, which binds to Chicken IgY (H&L) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Chicken IgYDyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on chicken IgG (IgY), light chains on all chicken immunoglobulinsNo reactivity is observed to: non-immunoglobulin chicken serum proteins
Goat anti-human IgA heavy (alpha chain) is a secondary antibody conjugated to HRP which binds to human IgA heavy (alpha chain) in immunological assays. It is especially adsorbed against bovine/mouse/rabbit serum and affinity purified.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Human IgA heavy chain (alpha)
Expected Species:
Human IgA Heavy chain (alpha)
Immunogen:
purified human IgA, heavy (alpha chain)
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
No reactivity is observed to the light chains or non-immunoglobulin human serum proteins.No reactivity to bovine, mouse or rabbit serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase
Rabbit anti-mouse IgG is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to all mouse immunoglobulins in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Rabbit
Species Reactivity:
Mouse IgG heavy and light chains (H&L)
Expected Species:
Mouse IgG Heavy and Light chains (H&L)
Immunogen:
Purified Mouse IgG, whole molecule
Applications:
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Vitale et al. (2021) Light Spectral Composition Influences Structural and Eco-Physiological Traits of Solanum lycopersicum L. cv. 'Microtom' in Response to High-LET Ionizing Radiation. Plants (Basel). 2021 Aug 23;10(8):1752. doi: 10.3390/plants10081752. PMID: 34451797; PMCID: PMC8399554.Bui et al. (2020). Differential submergence tolerance between juvenile and adult Arabidopsis plants involves the ANAC017 transcription factor. doi.org/10.1101/2020.02.12.945923 BioRxiv.Koch et al. (2019). Heat stress directly impairs gut integrity and recruits distinct immune cell populations into the bovine intestine. Proc Natl Acad Sci U S A. 2019 May 21;116(21):10333-10338. doi: 10.1073/pnas.1820130116.
Special application note:
Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.0)HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative.
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody concentration is 3.86 mg/ml and it has been afinity purified on solid phase hen IgY (H&L)biotin conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. It contains 0.05 % sodium azide as preservative
Goat anti-rabbit IgG (H&L) is a secondary antibody conjugated to AP or ALP (Alkaline phosphatase) which binds to all rabbit immunoglobulins in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid, clear, colorless.
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Rabbit IgG heavy and light chains (H&L)
Immunogen:
Purified Rabbit IgG, whole molecule,
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Based upon IEP, this antibody binds to: heavy chains on rabbit IgGlight chains on all rabbit immunoglobulinsNo reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Loudya et al. (2021) Cellular and transcriptomic analyses reveal two-staged chloroplast biogenesis underpinning photosynthesis build-up in the wheat leaf. Genome Biol. 2021 May 11;22(1):151. doi: 10.1186/s13059-021-02366-3. PMID: 33975629; PMCID: PMC8111775.Bapatla et al. (2021). Modulation of Photorespiratory Enzymes by Oxidative and Photo-Oxidative Stress Induced by Menadione in Leaves of Pea (Pisum sativum). Plants 10, no. 5: 987. https://doi.org/10.3390/plants10050987Szyma ?ska et al. (2019). SNF1-Related Protein Kinases SnRK2.4 and SnRK2.10 Modulate ROS Homeostasis in Plant Response to Salt Stress. Int J Mol Sci. 2019 Jan 2;20(1). pii: E143. doi: 10.3390/ijms20010143.Rozp ?dek et al. (2018). Acclimation of the photosynthetic apparatus and alterations in sugar metabolism in response to inoculation with endophytic fungi. Plant Cell Environ. 2018 Dec 5. doi: 10.1111/pce.13485.Borovik and Grabelnych (2018). Mitochondrial alternative cyanide-resistant oxidase is involved in an increase of heat stress tolerance in spring wheat. J Plant Physiol. 2018 Dec;231:310-317. doi: 10.1016/j.jplph.2018.10.007.
Special application note:
Concentration: 1.5mg/ml.Antibody has been affinity purified on solid phase rabbit IgG (H&L).AP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative
No reactivity is observed to non-immunoglobulin mouse serum proteins based in immunoelectrophoresis.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody concentration is 3.85 mg/ml and it has been afinity purified on solid phase sheep IgG (H&L)biotin conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. It contains 0.05 % sodium azide as preservative
Goat anti-chicken IgY (H&L) is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to chicken IgY in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Chicken IgY heavy and light chains (H&L).
Immunogen:
Purified chicken IgY, whole molecule
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Antibody binds to:heavy chains on chicken IgYlight chains on all chicken immunoglobulinsNo reactivity is observed to non-immunoglobulin chicken serum proteins based in immunoelectrophoresis.Chicken immunoglobulin is often called hen or chicken IgG, however it is derived from egg yolk, therefore IgY.
For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Bindari et al. (2020). Methods to prevent PCR amplification of DNA from non-viable virus were not successful for infectious laryngotracheitis virus. PLoS One. 2020 May 22;15(5):e0232571. doi: 10.1371/journal.pone.0232571. PMID: 32442180; PMCID: PMC7244108.Levitan et al. (2019). Structural and functional analyses of photosystem II in the marine diatom Phaeodactylum tricornutum. Proc Natl Acad Sci U S A. 2019 Aug 27;116(35):17316-17322. doi: 10.1073/pnas.1906726116.Heard et al. (2015). Identification of Regulatory and Cargo Proteins of Endosomal and Secretory Pathways in Arabidopsis thaliana by Proteomic Dissection. Mol Cell Proteomics. 2015 Jul;14(7):1796-813. doi: 10.1074/mcp.M115.050286. Epub 2015 Apr 21.Huang et al. (2015). Effects of exogenous salicylic acid on the physiological characteristics of Dendrobium officinale under chilling stress. Plant Growth Regulation pp 1-10.
Special application note:
Concentration: 1.0 mg/mlThis HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free 0.1 % (v/v) of Kathon CG is used as preservative.
Goat anti-Rat IgG (H&L), DyLight 488 Conjugated, min. cross-reactivity to human, mouse lgG is a secondary antibody conjugated to DyLight 488, which binds to Rat IgG (H&L) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Rat IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rat IgG, light chains on all rat immunoglobulinsNo reactivity is observed to: non-immunoglobulin mouse serum proteins, IgG from human or mouse
Goat anti-human IgA heavy (alpha chain), F(ab)'2 is a secondary antibody which binds to human IgA heavy (alpha chain), F(ab)'2 fragment in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life is one year from date of receipt.
No reactivity is observed to the light chains or non-immunoglobulin human serum proteins based on immunoelectrophoresis.Antibody reacts with the alpha chains on human IgA based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of Sodium azide as preservative.Antibody concentration is 3 mg/ml.Antibody purity ≥90% based on SDS-PAGE. May contain small amounts of intact IgG.
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0,5 mg of antibody in 0,55 ml of sterile water add 0,55 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG, light chains on all mouse immunoglobulinsNo reactivity is observed to: non-immunoglobulin mouse serum proteins
Goat anti-human IgA heavy (alpha chain) is a secondary antibody conjugated to HRP which binds to human IgA heavy (alpha chain) F(ab)'2 fragments in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Human IgA heavy chain (alpha) F(ab)'2 fragment
Expected Species:
Human IgA Heavy chain (alpha) F(ab)'2 fragment
Immunogen:
purified human IgA, heavy (alpha chain)
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
No reactivity is observed to the light chains or non-immunoglobulin human serum proteins
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 0,55 ml of sterile water
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidasePurity is ≥ 90% based on SDS-PAGE. May contain small amounts of intact IgG.
Donkey anti-Mouse IgG (H&L) - DyLight 650 Conjugated is a secondary antibody conjugated to DyLight 650, which binds to Mouse IgG (H&L) in immunological assays.DyLight 650 has Amax = 652 nm, Emax = 672 nm. Antibodies are purified using solid phase Mouse IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG light chains on all mouse immunoglobulinsNo reactivity is observed to: non-immunoglobulin mouse serum proteins
Donkey anti-Rat IgG (H&L), DyLight 350 Conjugated is a secondary antibody conjugated to DyLight 350, which binds to Rat IgG (H&L) in immunological assays.DyLight 350 has Amax = 353 nm, Emax = 432 nm. Antibodies are purified using solid phase Rat IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rat IgG, light chains on all rat immunoglobulinsNo reactivity is observed to: non-immunoglobulin rat serum proteins
Goat anti-human IgA (α chain) - DyLight 650 Conjugated is a secondary antibody conjugated to DyLight 650, which binds to human IgA (α chain) in immunological assays.DyLight 650 has Amax = 652 nm, Emax = 672 nm. Antibodies are are affinity purified using solid phase human IgA (α chain).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (α) chains on human IgA (α chain)No reactivity is observed to: non-immunoglobulin human serum proteins light chains on all human immunoglobulins
Rabbit anti-hamster IgG (H&L) - DyLight 633 Conjugated is a secondary antibody conjugated to DyLight 633, which binds to hamster IgG (H&L) in immunological assays. DyLight 633 has Amax = 638 nm, Emax = 658 nm. Antibodies are are affinity purified using solid phase hamster IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: (γ) chains on hamster IgG light chains on all hamster immunoglobulins.No reactivity is observed to:non-immunoglobulin hamster serum proteins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified rabbit IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Goat anti-guinea pig IgG (H&L) - DyLight 633 Conjugated is a secondary antibody conjugated to DyLight 633, which binds to guinea pig IgG (H&L) in immunological assays. DyLight 633 has Amax = 638 nm, Emax = 658 nm. Antibodies are are affinity purified using solid phase guinea pig IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: (γ) chains on guinea pig IgG light chains on all guinea pig immunoglobulins.No reactivity is observed to:non-immunoglobulin guinea pig serum proteins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Donkey anti-Goat IgG (H&L) - DyLight 633 Conjugated is a secondary antibody conjugated to DyLight 633, which binds to goat IgG (H&L) in immunological assays. DyLight 633 has Amax = 638 nm, Emax = 658 nm. Antibodies are are affinity purified using solid phase goat IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: chains on goat IgG light chains on all goat immunoglobulins.No reactivity is observed to: non-immunoglobulin goat serum proteins.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Donkey anti-Rat IgG (H&L) , DyLight 594 Conjugated, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human,mouse, rabbit or sheep IgG is a secondary antibody conjugated to DyLight 594, which binds to rat IgG (H&L) in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase rat IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rat IgG light chains on all rat immunoglobulins.No reactivity is observed to: non-immunoglobulin rat serum proteins IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit or sheep.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Goat anti-Mouse IgG Fc, FITC conjugated, min. cross-reactivity to bovine, horse or human serum proteins is a secondary antibody conjugated to FITC, which binds to mouse IgG Fc in immunological assays.
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Mouse IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on mouse IgG .Based on IEP, no reactivity is observed to: • non-immunoglobulin mouse serum proteins • light chains on all mouse immunoglobulins • mouse IgG, F(ab)'2 fragment • serum proteins from bovine, horse, or human .
Goat anti-Mouse IgG Fc, Biotin conjugated, min. cross-reactivity to bovine, horse or human serum proteins is a secondary antibody conjugated to biotin, which binds to mouse IgG Fc in immunological assays.
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Mouse IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on mouse IgG .Based on IEP, no reactivity is observed to: • non-immunoglobulin mouse serum proteins • light chains on all mouse immunoglobulins • mouse IgG, F(ab)'2 fragment • serum proteins from bovine, horse, or human .
Donkey anti-Rabbit IgG (H&L) - DyLight 633 Conjugated, min. cross-reactivity to human IgG and serum proteins is a secondary antibody conjugated to DyLight 633, which binds to rabbit IgG (H&L) in immunological assays. DyLight 633 has Amax = 638 nm, Emax = 658 nm. Antibodies are are affinity purified using solid phase rabbit IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: (γ) chains on rabbit IgG light chains on all rabbit immunoglobulins.No reactivity is observed to:non-immunoglobulin rabbit serum proteinsmouse IgG or serum proteins.
For reconstitution add 1,1 ml of sterile water,Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Mouse IgG + IgM.Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: • heavy chains on mouse IgG + IgM • light chains on all mouse immunoglobulins .Based on IEP, no reactivity is observed to: • non-immunoglobulin mouse serum proteins .
Donkey anti-Rabbit IgG (H&L) - DyLight 633 Conjugated is a secondary antibody conjugated to DyLight 633, which binds to rabbit IgG (H&L) in immunological assays.DyLight 633 has Amax = 638 nm, Emax = 658 nm. Antibodies are affinity purified using solid phase rabbit IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: (γ) chains on rabbit IgG light chains on all rabbit immunoglobulins.No reactivity is observed to:non-immunoglobulin rabbit serum proteins.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Antibody purity is > 90 % based on SDS-PAGE. Small amounts of intact IgG may be present.Affinity purified using solid phase Mouse IgG. F(ab)’2 fragment was prepared by pepsin digestion.Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2. 0.05 % (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: heavy (γ) chains on Mouse IgG.Based on IEP, no reactivity is observed to: light chains on all Mouse immunoglobulins, non-immunoglobulin Mouse serum proteins, Mouse IgG, F(ab)’2 fragment, serum proteins from Bovine, Horse, or Human.
Goat anti-mouse IgG (H&L) - F(ab)'2 fragment, DyLight 633 Conjugated is a secondary antibody conjugated to DyLight 633, which binds to mouse IgG, F(ab)'2 fragment in immunological assays. DyLight 633 has Amax = 638 nm, Emax = 658 nm. Antibodies are are affinity purified using solid phase mouse IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0,5 mg of antibody in 0,55 ml of sterile water add 0,55 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative. Based on immunoelectrophoresis, this antibody reacts with: (γ) chains on mouse IgG light chains on all mouse immunoglobulins.No reactivity is observed to:non-immunoglobulin mouse serum proteins.
Donkey anti-Rat IgG (H&L) - DyLight 633 Conjugated is a secondary antibody conjugated to DyLight 633, which binds to rat IgG (H&L) in immunological assays. DyLight 633 has Amax = 638 nm, Emax = 658 nm. Antibodies are are affinity purified using solid phase rat IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: (γ) chains on rat IgG light chains on all rat immunoglobulins.No reactivity is observed to:non-immunoglobulin rat serum proteins.
Goat anti-Mouse IgG + IgM, min. cross-reactivity to human IgG or serum proteins is a secondary antibody, which binds to mouse IgG + IgM in immunological assays.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Mouse IgG + IgM.Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: • heavy chains on mouse IgG + IgM • light chains on all mouse immunoglobulins .Based on IEP, no reactivity is observed to: • non-immunoglobulin mouse serum proteins • human IgG or serum proteins .
Donkey anti-Goat IgG (H&L), DyLight 800 Conjugated is a secondary antibody conjugated to DyLight 800, which binds to Goat IgG (H&L) in immunological assays.DyLight 800 has Amax = 777 nm, Emax = 794 nm. Antibodies are purified using solid phase Goat IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on goat IgG, light chains on all goat immunoglobulinsNo reactivity is observed to: non-immunoglobulin goat serum proteins.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Donkey anti-Sheep IgG (H&L), DyLight 800 Conjugated is a secondary antibody conjugated to DyLight 800, which binds to Sheep IgG (H&L) in immunological assays.DyLight 800 has Amax = 777 nm, Emax = 794 nm. Antibodies are purified using solid phase Sheep IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on sheep IgG, light chains on all sheep immunoglobulinsNo reactivity is observed to: non-immunoglobulin sheep serum proteins.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Donkey anti-Rat IgG (H&L), DyLight 800 Conjugated is a secondary antibody conjugated to DyLight 800, which binds to Rat IgG (H&L) in immunological assays.DyLight 800 has Amax = 777 nm, Emax = 794 nm. Antibodies are purified using solid phase Rat IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rat IgG, light chains on all rat immunoglobulinsNo reactivity is observed to: non-immunoglobulin rat serum proteins
Donkey anti-Sheep IgG (H&L), DyLight 350 Conjugated is a secondary antibody conjugated to DyLight 350, which binds to Sheep IgG (H&L) in immunological assays.DyLight 350 has Amax = 353 nm, Emax = 432 nm. Antibodies are purified using solid phase Sheep IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on sheep IgG, light chains on all sheep immunoglobulinsNo reactivity is observed to: non-immunoglobulin sheep serum proteins.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Mouse IgG + IgM.Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: • heavy chains on mouse IgG + IgM • light chains on all mouse immunoglobulins .Based on IEP, no reactivity is observed to: • non-immunoglobulin mouse serum proteins .
Rabbit anti-goat IgG (H&L), DyLight 800 Conjugated is a secondary antibody conjugated to DyLight 800, which binds to Goat IgG (H&L) in immunological assays.DyLight 800 has Amax = 777 nm, Emax = 794 nm. Antibodies are purified using solid phase Goat IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (��) chains on goat IgG, light chains on all goat immunoglobulinsNo reactivity is observed to: non-immunoglobulin goat serum proteins.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Goat anti-mouse IgG (H&L) - DyLight 633 Conjugated, min. cross-reactivity to bovine, goat, human, rabbit, rat IgG is a secondary antibody conjugated to DyLight 633, which binds to mouse IgG (H&L) in immunological assays. DyLight 633 has Amax = 638 nm, Emax = 658 nm. Antibodies are are affinity purified using solid phase mouse IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative. Based on immunoelectrophoresis, this antibody reacts with: (γ) chains on mouse IgG light chains on all mouse immunoglobulins.No reactivity is observed to:non-immunoglobulin mouse serum proteins IgG from bovine, goat, human, rabbit or rat.
Donkey anti-Mouse IgG (H&L), DyLight 800 Conjugated is a secondary antibody conjugated to DyLight 800, which binds to Mouse IgG (H&L) in immunological assays.DyLight 800 has Amax = 777 nm, Emax = 794 nm. Antibodies are purified using solid phase Mouse IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG, light chains on all mouse immunoglobulinsNo reactivity is observed to: non-immunoglobulin mouse serum proteins
Rabbit anti-goat IgG (H&L), DyLight 800 Conjugated, min. cross-reactivity to human, mouse, rat lgG is a secondary antibody conjugated to DyLight 800, which binds to Goat IgG (H&L) in immunological assays.DyLight 800 has Amax = 777 nm, Emax = 794 nm. Antibodies are purified using solid phase Goat IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on goat IgG, light chains on all goat immunoglobulinsNo reactivity is observed to: non-immunoglobulin goat serum proteins, IgG from human, mouse, or rat
Rabbit anti-human IgG (H&L), DyLight 800 Conjugated is a secondary antibody conjugated to DyLight 800, which binds to human IgG (H&L) in immunological assays.DyLight 800 has Amax = 777 nm, Emax = 794 nm. Antibodies are purified using solid phase Human IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on human IgG, light chains on all human immunoglobulinsNo reactivity is observed to: non-immunoglobulin human serum proteins
Goat anti-human IgG (H&L), DyLight 800 Conjugated, min. cross-reactivity to bovine, mouse, rabbit serum is a secondary antibody conjugated to DyLight 800, which binds to human IgG (H&L) in immunological assays.DyLight 800 has Amax = 777 nm, Emax = 794 nm. Antibodies are purified using solid phase Human IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on human IgG, light chains on all human immunoglobulinsNo reactivity is observed to: non-immunoglobulin human serum proteins, serum proteins from bovine, mouse or rabbit
Donkey anti-Mouse IgG (H&L), F(ab)'2 Fragment, TRITC conjugated is a secondary antibody conjugated to TRITC, which binds to mouse IgG (H&L), F(ab)'2 Fragment in immunological assays.
For reconstitution add 0,55 ml of sterile water,Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Antibody purity is > 90% based on SDS-PAGE Small amounts of intact IgG may be present.Affinity purified using solid phase Mouse IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on mouse IgG • light chains on all mouse immunoglobulins .Based on IEP, no reactivity is observed to: • non-immunoglobulin mouse serum immunoglobulins .
Goat anti-mouse IgG (H&L) - DyLight 633 Conjugated, min. cross-reactivity to bovine, horse, human, pig or rabbit serum proteins is a secondary antibody conjugated to DyLight 633, which binds to mouse IgG (H&L) in immunological assays. DyLight 633 has Amax = 638 nm, Emax = 658 nm. Antibodies are are affinity purified using solid phase mouse IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative. Based on immunoelectrophoresis, this antibody reacts with: (γ) chains on mouse IgG light chains on all mouse immunoglobulins.No reactivity is observed to:non-immunoglobulin mouse serum proteins serum proteins from bovine, horse, human, pig, or rabbit.
Goat anti-mouse IgG (H&L) - DyLight 650 Conjugated, min. cross-reactivity to bovine, horse, human, pig or rabbit serum proteins is a secondary antibody conjugated to DyLight 650, which binds to Mouse IgG (H&L) in immunological assays.DyLight 650 has Amax = 652 nm, Emax = 672 nm. Antibodies are purified using solid phase Mouse IgGDyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG light chains on all mouse immunoglobulinsNo reactivity is observed to: non-immunoglobulin mouse serum proteins serum proteins from bovine, horse, human, pig, or rabbit
Donkey anti-Mouse IgG (H&L) - F(ab)'2 fragment, DyLight 633 Conjugated is a secondary antibody conjugated to DyLight 633, which binds to mouse IgG (H&L), F(ab)'2 fragment in immunological assays. DyLight 633 has Amax = 638 nm, Emax = 658 nm. Antibodies are are affinity purified using solid phase mouse IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0,5 mg of antibody in 0,55 ml of sterile water add 0,55 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Donkey anti-Mouse IgG (H&L) - DyLight 633 Conjugated, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep IgG is a secondary antibody conjugated to DyLight 633, which binds to mouse IgG (H&L) in immunological assays. DyLight 633 has Amax = 638 nm, Emax = 658 nm. Antibodies are are affinity purified using solid phase mouse IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: (γ) chains on mouse IgG light chains on all mouse immunoglobulins.No reactivity is observed to:non-immunoglobulin mouse serum proteins IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Donkey anti-Mouse IgG (H&L) - DyLight 633 Conjugated is a secondary antibody conjugated to DyLight 633, which binds to mouse IgG (H&L) in immunological assays. DyLight 633 has Amax = 638 nm, Emax = 658 nm. Antibodies are are affinity purified using solid phase mouse IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: (γ) chains on mouse IgG light chains on all mouse immunoglobulins.No reactivity is observed to:non-immunoglobulin mouse serum proteins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Goat anti-human IgG Fc - DyLight 633 Conjugated is a secondary antibody conjugated to DyLight 633, which binds to human IgG Fc in immunological assays. DyLight 633 has Amax = 638 nm, Emax = 658 nm. Antibodies are are affinity purified using solid phase human IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: (γ) chains on human IgG.No reactivity is observed to:non-immunoglobulin human serum proteins light chains on all human immunoglobulins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Donkey anti-Mouse IgG (H&L), DyLight 350 Conjugated is a secondary antibody conjugated to DyLight 350, which binds to Mouse IgG (H&L) in immunological assays.DyLight 350 has Amax = 353 nm, Emax = 432 nm. Antibodies are purified using solid phase Mouse IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG, light chains on all mouse immunoglobulinsNo reactivity is observed to: non-immunoglobulin mouse serum proteins
Goat anti-human IgG Fc - DyLight 650 Conjugated, min. cross-reactivity to bovine, horse, mouse, rabbit serum proteins is a secondary antibody conjugated to DyLight 650, which binds to human IgG Fc in immunological assays.DyLight 650 has Amax = 652 nm, Emax = 672 nm. Antibodies are are affinity purified using solid phase human IgG Fc.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on human IgGNo reactivity is observed to: non-immunoglobulin human serum proteins light chains on all human immunoglobulins serum proteins from bovine, mouse or rabbit
Goat anti-human IgG (H&L) - DyLight 650 Conjugated, min. cross-reactivity to bovine, mouse, rabbit serum is a secondary antibody conjugated to DyLight 650, which binds to human IgG (H&L) in immunological assays.DyLight 650 has Amax = 652 nm, Emax = 672 nm. Antibodies are are affinity purified using solid phase human IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on human IgG. Light chains on all human immunoglobulinsNo reactivity is observed to: non-immunoglobulin human serum proteins serum proteins from bovine, mouse or rabbit
Donkey anti-Sheep IgG (H&L) , DyLight 594 Conjugate is a secondary antibody conjugated to DyLight 594, which binds to all sheep IgG (H&L) in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase sheep IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on sheep IgG light chains on all sheep immunoglobulins.No reactivity is observed to: non-immunoglobulin sheep serum proteins.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Donkey anti-Rabbit IgG (H&L), DyLight 350 Conjugated is a secondary antibody conjugated to DyLight 350, which binds to Rabbit IgG (H&L) in immunological assays.DyLight 350 has Amax = 353 nm, Emax = 432 nm. Antibodies are purified using solid phase Rabbit IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG, light chains on all rabbit immunoglobulinsNo reactivity is observed to: non-immunoglobulin rabbit serum proteins
Donkey anti-Mouse IgG (H&L), F(ab)'2 Fragment, FITC conjugated is a secondary antibody conjugated to FITC, which binds to mouse IgG (H&L), F(ab)'2 Fragment in immunological assays.
Antibody purity is > 90% based on SDS-PAGE Small amounts of intact IgG may be present.Affinity purified using solid phase Mouse IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on mouse IgG • light chains on all mouse immunoglobulins .Based on IEP, no reactivity is observed to: • non-immunoglobulin mouse serum immunoglobulins .
Goat anti-Rabbit IgG (H&L), min. cross-reactivity to bovine, goat, human, mouse or rat IgG or serum is a secondary antibody which binds to rabbit IgG in immunological assays.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Rabbit IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on rabbit IgG • light chains on all rabbit immunoglobulins .Based on IEP, no reactivity is observed to: • non-immunoglobulin rabbit serum proteins • serum proteins from bovine, goat, human, mouse, or rat • IgG from bovine, goat, human, mouse or rat .
Donkey anti-Sheep IgG (H&L) - DyLight 633 Conjugated is a secondary antibody conjugated to DyLight 633, which binds to sheep IgG (H&L) in immunological assays. DyLight 633 has Amax = 638 nm, Emax = 658 nm. Antibodies are are affinity purified using solid phase sheep IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: (γ) chains on sheep IgG light chains on all sheep immunoglobulins.No reactivity is observed to:non-immunoglobulin sheep serum proteins.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Rabbit anti-Rat IgG (H&L) - DyLight 633 Conjugated is a secondary antibody conjugated to DyLight 633, which binds to rat IgG (H&L) in immunological assays. DyLight 633 has Amax = 638 nm, Emax = 658 nm. Antibodies are are affinity purified using solid phase rat IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: (γ) chains on rat IgG light chains on all rat immunoglobulins.No reactivity is observed to:non-immunoglobulin rat serum proteins.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified donkey IgG.
Special application note:
Antibody purity is > 90% based on SDS-PAGE Small amounts of intact IgG may be present.Affinity purified using solid phase Rabbit IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on rabbit IgG • light chains on all rabbit immunoglobulins .Based on IEP, no reactivity is observed to: • non-immunoglobulin rabbit serum proteins .
Goat anti-rabbit IgG (H&L) - F(ab)'2 fragment, DyLight 594 Conjugated, min. cross-reactivity to bovine, human, or mouse lgG or serum proteins is a secondary antibody conjugated to DyLight 594, which binds to all rabbit IgG (H&L), F(ab)'2 fragment in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase rabbit IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0,5 mg of antibody in 0,55 ml of sterile water add 0,55 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG light chains on all rabbit immunoglobulins.No reactivity is observed to: non-immunoglobulin rabbit serum proteins IgG from bovine, human or mouse serum proteins from bovine, human or mouse.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Rabbit anti-mouse IgG (H&L) - DyLight 650 Conjugated is a secondary antibody conjugated to DyLight 650, which binds to mouse IgG (H&L) in immunological assays.DyLight 650 has Amax = 652 nm, Emax = 672 nm. Antibodies are purified using solid phase Mouse IgGDyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG light chains on all mouse immunoglobulinsNo reactivity is observed to: non-immunoglobulin mouse serum proteins
Goat anti-rabbit IgG (H&L) , DyLight 594 Conjugated, min. cross-reactivity to bovine, goat, human, mouse, rat IgG or serum proteins is a secondary antibody conjugated to DyLight 594, which binds to all rabbit IgG (H&L) in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase rabbit IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG light chains on all rabbit immunoglobulins.No reactivity is observed to: non-immunoglobulin rabbit serum proteins serum proteins from bovine, goat, human, mouse or rat IgG from bovine, goat, human, mouse or rat.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Rabbit anti-goat IgG (H&L) - DyLight 650 Conjugated is a secondary antibody conjugated to DyLight 650, which binds to Goat IgG (H&L) in immunological assays.DyLight 650 has Amax = 652 nm, Emax = 672 nm. Antibodies are are affinity purified using solid phase goat IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on goat IgG light chains on all goat immunoglobulinsNo reactivity is observed to: non-immunoglobulin goat serum proteins.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Donkey IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on donkey IgG • light chains on all donkey immunoglobulins .Based on IEP, no reactivity is observed to: • non-immunoglobulin donkey serum immunoglobulins .
Donkey anti-Rabbit IgG (H&L) - DyLight 650 Conjugated is a secondary antibody conjugated to DyLight 650, which binds to Rabbit IgG (H&L) in immunological assays.DyLight 650 has Amax = 652 nm, Emax = 672 nm. Antibodies are purified using solid phase Rabbit IgGDyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG light chains on all rabbit immunoglobulinsNo reactivity is observed to: non-immunoglobulin rabbit serum proteins
Donkey anti-Rabbit IgG (H&L), DyLight 350 Conjugated, min. cross-reactivity to human IgG and serum proteins is a secondary antibody conjugated to DyLight 350, which binds to Rabbit IgG (H&L) in immunological assays.DyLight 350 has Amax = 353 nm, Emax = 432 nm. Antibodies are purified using solid phase Rabbit IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG, light chains on all rabbit immunoglobulinsNo reactivity is observed to: non-immunoglobulin rabbit serum proteins, mouse IgG or serum proteins
Goat anti-mouse IgG (H&L), DyLight 350 Conjugated, min. cross-reactivity to bovine, horse, human, pig or rabbit serum proteins is a secondary antibody conjugated to DyLight 350, which binds to Mouse IgG (H&L) in immunological assays.DyLight 350 has Amax = 353 nm, Emax = 432 nm. Antibodies are purified using solid phase mouse IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG, light chains on all mouse immunoglobulinsNo reactivity is observed to: non-immunoglobulin mouse serum proteins, serum proteins from bovine, horse, human, pig, or rabbit
Donkey anti-Goat IgG (H&L) - DyLight 650 Conjugated is a secondary antibody conjugated to DyLight 650, which binds to Goat IgG (H&L) in immunological assays.DyLight 650 has Amax = 652 nm, Emax = 672 nm. Antibodies are are affinity purified using solid phase goat IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on goat IgG light chains on all goat immunoglobulinsNo reactivity is observed to: non-immunoglobulin goat serum proteins.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Chicken anti-Goat IgG (H&L) - DyLight 633 Conjugated, min. cross-reactivity to human, mouse or rabbit IgG and serum proteins is a secondary antibody conjugated to DyLight 633, which binds to goat IgG (H&L) in immunological assays. DyLight 633 has Amax = 638 nm, Emax = 658 nm. Antibodies are are affinity purified using solid phase goat IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0,5 mg of antibody in 0,55 ml of sterile water add 0,55 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: chains on goat IgG light chains on all goat immunoglobulins.No reactivity is observed to:non-immunoglobulin goat serum proteinsIgG/serum from human, mouse or rabbit.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified chicken IgG.
Reconstitution:
For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Goat anti-guinea pig IgG (H&L) - DyLight 650 Conjugated is a secondary antibody conjugated to DyLight 650, which binds to Guinea pig IgG (H&L) in immunological assays.DyLight 650 has Amax = 652 nm, Emax = 672 nm. Antibodies are are affinity purified using solid phase guinea pig IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on guinea pig IgG light chains on all guinea pig immunoglobulinsNo reactivity is observed to: non-immunoglobulin guinea pig serum proteins
Goat anti-rabbit IgG Fc, FITC (fluorescein-5-isothiocyanate) conjugated, is a secondary antibody which binds to rabbit IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Antibodies are adsorbed against bovine, horse and human serum proteins and affinity purified using solid phase rabbit IgG Fc.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Rabbit IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with:• heavy (γ) chains on rabbit IgG Based on IEP, no reactivity is observed to: • non-immunoglobulin rabbit serum proteins • light chains on all rabbit immunoglobulins • Rabbit IgG F(ab)'2 fragment • serum proteins from bovine, horse or human
Donkey anti-Sheep IgG (H&L) , DyLight 594 Conjugated, min. cross-reactivity to human,mouse, or rabbit IgG is a secondary antibody conjugated to DyLight 594, which binds to all sheep IgG (H&L) in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase sheep IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on sheep IgG light chains on all sheep immunoglobulins.No reactivity is observed to: non-immunoglobulin sheep serum proteins IgG from human, mouse or rabbit.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Donkey anti-Sheep IgG (H&L) , DyLight 594 Conjugated, min. cross-reactivity to human or rabbit IgG is a secondary antibody conjugated to DyLight 594, which binds to all sheep IgG (H&L) in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase sheep IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on sheep IgG light chains on all sheep immunoglobulins.No reactivity is observed to: non-immunoglobulin sheep serum proteins IgG from human or rabbit.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
No reactivity is observed to non-immunoglobulin guinea pig serum proteins based in immunoelectrophoresis.This antibody reacts with the heavy chains on guinea pig IgG and with the light chains on all guinea pig immunoglobulins based on immunoelectrophoresis.
Goat anti-guinea pig IgG is a secondary antibody conjugated to HRP which binds to all guinea pig IgGs in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Guinea pig IgG heavy and light chains (H&L) of all guinea pig immunoglobulins
Expected Species:
Guinea Pig IgG Heavy and Light chains (H&L) of all Guinea Pig immunoglobulins
Immunogen:
Purified Guinea pig IgG, whole molecule
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
No reactivity is observed to non-immunoglobulin guinea pig serum proteins, No reactivity is observed to serum from bovine, hen, goat, hamster, horse, human, mouse, rabbit, rat or sheep based on immunoelecrophoresis
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative.
Goat anti-guinea pig IgG is a secondary antibody conjugated to HRP which binds to all guinea pig IgGs in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Heavy chains on guinea pig IgG and light chains on all guinea pig immunoglobulins
Immunogen:
Purified Guinea pig IgG, whole molecule
Applications:
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily
Not reactive in:
Non-immunoglobulin guinea pig serum proteins
Selected references:
Jin et al. (2019). YAP activation promotes the transdifferentiation of cardiac fibroblasts to myofibroblasts in matrix remodeling of dilated cardiomyopathy. Brazilian Journal of Medical and Biological Research (2019) 52(1): e7914.
Special application note:
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 10 % (w/v) BSA, Protease/IgG free with 0,1 % (v/v) of Kathon CG as preservative
Donkey anti-rabbit IgG (H&L) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to rabbit IgG (H&L) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Donkey
Species Reactivity:
Rabbit IgG (H&L)
Expected Species:
Rabbit IgG (H&L)
Immunogen:
Purified Rabbit IgG
Applications:
ELISA (ELISA) , Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
This antibody reacts with alpha (heavy chains) on rabbit IgG and all light chains on rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Kay et al. (2014). Elevations in Th2-initiating cytokines (IL-33, IL-25, TSLP) in lesional skin from Chronic Spontaneous ("Idiopathic") Urticaria. Br J Dermatol. 2014 Dec 18. doi: 10.1111/bjd.13621.
Special application note:
ALP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified rabbit IgG.
Not reactive in:
Based on IEP, no reactivity is observed to:˖ non-immunoglobulin rabbit serum proteins
Special application note:
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of Sodium azide as preservative.Antibody reacts with: heavy (γ) chains chains on rabbit IgG light chains on all on all rabbit immunoglobulinsBased on IEP, no reactivity is observed to non-immunoglobulin rabbit serum proteins
Goat anti-rabbit IgG is a secondary antibody conjugated to HRP which binds to rabbit IgGs in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
heavy chains of Rabbit IgG, Light chains on all Rabbit immunoglobulins
Expected Species:
Rabbit IgG Heavy and Light chains (H&L) of all Rabbit immunoglobulins
Immunogen:
purified Rabbit IgG, whole molecule
Applications:
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
Based on immunoelectrophoresis no reactivity is observed to:non-immunoglobulin rabbit serum proteinsserum from bovine, human or mouseIgG from human or mouse
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Lacour et al. (2019). Decoupling light harvesting, electron transport and carbon fixation during prolonged darkness supports rapid recovery upon re-illumination in the Arctic diatom Chaetoceros neogracilis. Polar Biol (2019). https://doi.org/10.1007/s00300-019-02507-2.
Special application note:
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase.Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.0)
Goat anti-rabbit IgG is a secondary antibody conjugated to HRP which binds to all rabbit IgGs in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Rabbit IgG heavy and light chains (H&L)
Expected Species:
Rabbit IgG Heavy and Light chains (H&L)
Immunogen:
Purified Rabbit IgG, whole molecule (H&L chains)
Applications:
Immunohistochemistry (IHC), ELISA (ELISA), Western blot (WB)
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
No reactivity is observed to bovine/goat/human/mouse or rat IgG based on immunoelectrophoresis.No reactivity to non-immunoglobulin rabbit serum proteins.Antibody is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.1 % (v/v)Kathon CG is added as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase.
Goat anti-human IgG Fc is a secondary antibody conjugated to HRP which binds to human Fc part of IgG in immunological assays. The antibody is absorbed agains human IgA and IgM
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Human IgG heavy chain Fc based on immunoelectrophoresis
Expected Species:
Human IgG Fc
Immunogen:
affinity purified human IgG Fc part
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative.
Rabbit anti-goat IgG is a secondary antibody conjugated to HRP which binds to goat IgGs in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Rabbit
Species Reactivity:
Goat IgG heavy chains and light chains on all Goat immunoglobulins
Expected Species:
Goat IgG Heavy chains and Light chains on all Goat immunoglobulins
Immunogen:
Purified Goat IgG, whole molecule
Applications:
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
No reactivity is observed to non-immunoglobulin goat serum proteins. No reactivity is observed to serum from human, mouse and rat based on immunoelecrophoresis.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative.
No reactivity is observed to non-immunoglobulin goat serum proteins based in immunoelectrophoresis.No reactivity is observed to human, mouse and rat IgG based on immunoelectrophoresis.
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Biotin conjugate is supplied in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, Contains 0,05 % (w/v) sodium azide as preservativeAntibody binds to heavy chains of goat IgG and light chains of all goat immunoglobulins based on immunoelectrophoresis,?BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG
Rabbit anti-hamster IgG (H&L) is a secondary antibody conjugated to ALP, which binds to hamster IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase hamster IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard. Shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified rabbit IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.50 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 30 mM triethanolamine, pH 7.2.5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on hamster IgG, light chains on allhamster immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin hamster serum proteins.
Goat anti-guinea pig IgG is a secondary antibody conjugated to ALP (alkaline phosphatase) which binds to all guinea pig immunoglobulins in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilution prior to use and then discard. Shelf life of this product is one year from date of receipt.
Host Animal:
Goat
Expected Species:
Guinea Pig IgG Heavy and Light chains (H&L)
Immunogen:
Purified Guinea pig IgG, whole molecule
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
No reactivity is observed to non-immunoglobulin guinea pig serum proteins based in immunoelectrophoresis. This antibody reacts with the heavy chains on guinea pig IgG and with the light chains on all guinea pig immunoglobulins based on immunoelectrophoresis.
No confirmed exceptions from predicted reactivity are currently known
Special application note:
ALP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified mouse IgG.
Not reactive in:
Based on IEP, no reactivity is observed to:˖ non-immunoglobulin mouse serum proteins
Special application note:
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of Sodium azide as preservative.Antibody reacts with: heavy (γ) chains chains on mouse IgG light chains on all on all mouse immunoglobulinsBased on IEP, no reactivity is observed to:˖ non-immunoglobulin mouse serum proteins
Rabbit anti-mouse IgG (H&L), DyLight 488 Conjugated is a secondary antibody conjugated to DyLight 488, which binds to Mouse IgG (H&L) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Mouse IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG, light chains on all mouse immunoglobulinsNo reactivity is observed to: non-immunoglobulin mouse serum proteins
Goat anti-chicken IgY, DyLight 350 Conjugate is a secondary antibody conjugated to DyLight 350, which binds to hen immunoglobulins in immunological assays.DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis this antibody reacts with heavy chains on chicken IgG and light chains on all chicken immunoglobulins.No reactivity is observed to non-immunoglobulin chicken serum proteins based in immunoelectrophoresis.
Application Details:
1 : 50 -1 : 5 000 (ICC), 1 : 20- 1 : 2000 (IHC)
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.DyLight 350 (Ex = 353 nm; Em = 432 nm).
Goat anti-chicken IgY, DyLight 550 Conjugate is a secondary antibody conjugated to DyLight 550, which binds to hen immunoglobulins in immunological assays. DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis this antibody reacts with heavy chains on chicken IgG and light chains on all chicken immunoglobulins.No reactivity is observed to non-immunoglobulin chicken serum proteins based in immunoelectrophoresis.
Application Details:
1 : 50 -1 : 5 000 (ICC), 1 : 20- 1 : 2000 (IHC)
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.DyLight 550 (Ex = 550 nm; Em = 576 nm).
Goat anti-chicken IgY, DyLight 594 Conjugate is a secondary antibody conjugated to DyLight 594, which binds to hen immunoglobulins in immunological assays. DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis this antibody reacts with heavy chains on chicken IgG and light chains on all chicken immunoglobulins.No reactivity is observed to non-immunoglobulin chicken serum proteins based in immunoelectrophoresis.
Application Details:
1 : 50-1 : 5 000 (ICC), 1 : 20 -1 : 2000 (IHC)
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1 ml of sterile water, Allow reconstituted product to stand for at least 30 minutes at room temperature prior to dilution, If necessary, centrifuge to remove any particulates, Prepare fresh working dilution daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.DyLight 594 (Ex = 593 nm; Em = 618 nm).
Goat anti-chicken IgY, DyLight 633 Conjugate is a secondary antibody conjugated to DyLight 633, which binds to hen immunoglobulins in immunological assays. DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis this antibody reacts with heavy chains on chicken IgG and light chains on all chicken immunoglobulins.No reactivity is observed to non-immunoglobulin chicken serum proteins based in immunoelectrophoresis.
Application Details:
1 : 50 -1 : 5 000 (ICC), 1 : 20- 1 : 2000 (IHC)
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.DyLight 633 (Ex = 638 nm; Em = 658 nm).
Goat anti-chicken IgY, DyLight 650 Conjugate is a secondary antibody conjugated to DyLight 650, which binds to hen immunoglobulins in immunological assays. DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis this antibody reacts with heavy chains on chicken IgY and light chains on all chicken immunoglobulins.No reactivity is observed to non-immunoglobulin chicken serum proteins based in immunoelectrophoresis.
Application Details:
1 : 50 -1 : 5 000 (ICC), 1 : 20- 1 : 2000 (IHC)
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.DyLight 650 (Ex = 652 nm; Em = 672 nm).Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.0)
Goat anti-chicken IgY, DyLight 800 Conjugate is a secondary antibody conjugated to DyLight 800, which binds to hen immunoglobulins in immunological assays. DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis this antibody reacts with heavy chains on chicken IgY and light chains on all chicken immunoglobulins.No reactivity is observed to non-immunoglobulin chicken serum proteins based in immunoelectrophoresis.
Application Details:
1 : 50 -1 : 5 000 (ICC), 1 : 20- 1 : 2000 (IHC)
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.DyLight 800 (Ex = 777 nm; Em = 794 nm).
Goat anti-mouse IgM, chain is a HRP conjugated secondary antibody which binds to mouse IgM, chain in immunological assays. It has been specially adsorbed against human IgG/serum. Antibodies are affinity purified using solid phase mouse IgM.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on mouse IgM based on immunoelectrophoresis.Minimum cross-reactivity is observed with the light chains based on immunoelectrophoresis. Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins or to human IgG or serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.
Donkey anti-sheep IgG (H&L), Rhodamine (TRITC-tetramethylrhodamine-5-isothiocyanate) conjugated is a secondary antibody which binds to sheep IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are affinity purified using solid phase sheep IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°CShelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on sheep IgG and with the light chains on all sheep immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin sheep serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Normal goat serum lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. This product is used as a blocking reagent or control for most immunoassay applications.
Product Type:
Antibody
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long term storage after reconstitution, prepare small aliquots and store at -20 °C. For storage at 2-8 C, add a preservative to prevent growth of bacteria. Rehydrate with 10,0 ml of deionized water. Swirl gentle and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates.
Protein concentration is 60,0 mg/ml (Bradford, IgG standards), Antibody is supplied in 10 mM sodium phosphate, 0,15 M sodium chloride, pH 7,2, No preservative is added
Donkey anti-Goat IgG (H&L) - DyLight 550 Conjugated is a secondary antibody conjugated to DyLight 550, which binds to all goat IgGs in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase goat IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy chains on goat IgG light chains on all goat immunoglobulins.No reactivity is observed to: non-immunoglobulin goat serum proteins.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Donkey anti-Goat IgG (H&L) - DyLight 550 Conjugated, min cross reactivity to human, mouse, rabbit or rat IgG is a secondary antibody conjugated to DyLight 550, which binds to all goat IgGs in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase goat IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on goat IgG light chains on all goat immunoglobulins.No reactivity is observed to: non-immunoglobulin goat serum proteins IgG/serum from human, mouse, rabbit or rat.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Normal goat serum lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. This product is used as a blocking reagent or control for most immunoassay applications.
Product Type:
Antibody
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long term storage after reconstitution, prepare small aliquots and store at -20 °C. For storage at 2-8 C, add a preservative to prevent growth of bacteria. Rehydrate with 10,0 ml of deionized water. Swirl gentle and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates.
Pastula & Lundmark (2021). Induction of Epithelial-mesenchymal Transition in MDCK II Cells. Bio-protocol 11(3): e3903. DOI: 10.21769/BioProtoc.3903.
Special application note:
Protein concentration is 60,0 mg/ml (Bradford, IgG standards), Antibody is supplied in 10 mM sodium phosphate, 0,15 M sodium chloride, pH 7,2, No preservative is added
Rabbit anti-goat IgG (H&L) - DyLight 550 Conjugated, min cross reactivity to human, mouse, rat lgG is a secondary antibody conjugated to DyLight 550, which binds to all goat IgGs in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase goat IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on goat IgG light chains on all goat immunoglobulin.No reactivity is observed to: non-immunoglobulin goat serum proteins IgG from human, mouse, or rat.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified rabbit IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Normal rabbit serum lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. This product is used as a blocking reagent or control for most immunoassay applications.
Product Type:
Antibody
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long term storage after reconstitution, prepare small aliquots and store at -20 °C. For storage at 2-8 C, add a preservative to prevent growth of bacteria. Rehydrate with 10,0 ml of deionized water. Swirl gentle and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates.
Protein concentration is 60.0 mg/ml (Bradford, IgG standards). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. No preservative is added.Rabbit serum can be used as a blocking reagent at 2-5% for primary antibodies developed in a rabbit. This product can be also used in flow cytometry. Serum has been delipidated and filtered using 0.22 m filter.
Goat anti-guinea pig IgG (H&L) - DyLight 550 Conjugate is a secondary antibody conjugated to DyLight 550, which binds to all guinea pig IgG in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase guinea pig IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on guinea pig IgG light chains on all guinea pig immunoglobulins.No reactivity is observed to: non-immunoglobulin guinea pig serum proteins
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Donkey anti-Rat IgG (H&L), DyLight 488 Conjugated is a secondary antibody conjugated to DyLight 488, which binds to Rat IgG (H&L) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Rat IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Selected references:
Calvo-Polanco et al. (2014). Mild Salt Stress Conditions Induce Different Responses in Root Hydraulic Conductivity of Phaseolus vulgaris Over-Time. PLoS One. 2014 Mar 4;9(3):e90631. doi: 10.1371/journal.pone.0090631. eCollection 2014.
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rat IgG, light chains on all rat immunoglobulinsNo reactivity is observed to: non-immunoglobulin rat serum proteins
Sheep anti-rabbit IgG (H&L) is a secondary antibody conjugated to TRITC, which binds to rabbit IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rabbit IgG (H&L). Tetramethylrhodamine-5-isothiocyanate (TRITC) has Amax = 550 nm; Emax = 570 nm.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified sheep IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rabbit IgG, light chains on all rabbit immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rabbit serum proteins.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Not reactive in:
Based on IEP, no reactivity is observed to non-immunoglobulin chicken serum proteins
Special application note:
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of Sodium azide as preservative.Concentration: > 4.5 mg/ml (E 1% at 280 nm = 14.0)
No reactivity is observed to non-immunoglobulin proteins from guinea pig serum
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of Sodium azide as preservative.Antibody concentration is 4.5 mg/ml.
Goat anti-human IgA heavy (alpha chain) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgA alpha chains in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Human IgA heavy (alpha chains)
Expected Species:
Human IgA Heavy (alpha chains)
Immunogen:
purified human IgA, alpha chain
Applications:
ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
No confirmed exceptions from predicted reactivity are currently known
Special application note:
AP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
Goat anti-human IgA heavy (alpha chain) is a secondary antibody conjugated to HRP which binds to human IgA heavy (alpha chain) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Human IgA heavy (alpha chain)
Expected Species:
Human IgA Heavy (alpha chain)
Immunogen:
purified human IgA, heavy (alpha chain)
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
No reactivity is observed to the light chains or non-immunoglobulin human serum proteins
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase
Goat anti-human IgA (α chain) - DyLight 550 Conjugate, min cross reactivity to bovine, mouse, rabbit serum is a secondary antibody conjugated to DyLight 550, which binds to all human IgA (α chain) in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase human IgA.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (α) chains on human IgA (α chain)No reactivity is observed to: non-immunoglobulin human serum proteins light chains on all human immunoglobulins serum proteins from bovine, mouse or rabbit.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Goat anti-human IgG Fc - DyLight 550 Conjugated is a secondary antibody conjugated to DyLight 550, which binds to all human IgG, Fc in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase human IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on human IgG.No reactivity is observed to: non-immunoglobulin human serum proteins light chains on all human immunoglobulins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Goat anti-human IgM ( chain) - DyLight 550 Conjugated, min. cross-reactivity to human IgA + IgG is a secondary antibody conjugated to DyLight 550, which binds to all human IgM ( chain) in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase human IgM.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy ( ) chains on human IgM.No reactivity is observed to: non-immunoglobulin human serum proteins light chains on all human immunoglobulins human IgA + IgG.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Chicken anti-Mouse IgG (H&L) - DyLight 550 Conjugated, min. cross reactivity to human or rabbit IgG and serum proteins is a secondary antibody conjugated to DyLight 550, which binds to all mouse IgG in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase mouse IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0,5 mg of antibody in 0,55 ml of sterile water add 0,55 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG light chains on all mouse immunoglobulins.No reactivity is observed to: non-immunoglobulin mouse serum proteins IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep.
Application Details:
1 : 20-1 : 2000 for most applications,1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified chicken IgG.
Reconstitution:
For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Donkey anti-Mouse IgG (H&L) - DyLight 550 Conjugated, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep IgG is a secondary antibody conjugated to DyLight 550, which binds to all mouse IgG (H&L) in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase mouse IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8 C, Product is stable for 4 weeks at 2-8°Cafter rehydration,For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity, For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol, Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol, Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG light chains on all mouse immunoglobulins.No reactivity is observed to: non-immunoglobulin mouse serum proteins IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Goat anti-mouse IgG (H&L) - DyLight 550 Conjugated, min. cross-reactivity to bovine, goat, human, rabbit, rat IgG is a secondary antibody conjugated to DyLight 550, which binds to all mouse IgG (H&L) in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase mouse IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG light chains on all mouse immunoglobulins.No reactivity is observed to: non-immunoglobulin mouse serum proteins IgG from bovine, goat, human, rabbit or rat.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Goat anti-mouse IgG (H&L) - F(ab)'2 fragment, DyLight 550 Conjugate, min. cross-reactivity to bovine, horse, human, pig or rabbit serum proteins is a secondary antibody conjugated to DyLight 550, which binds to all mouse IgG (H&L), F(ab)'2 fragment in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase mouse IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0,5 mg of antibody in 0,55 ml of sterile water add 0,55 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG light chains on all mouse immunoglobulins.No reactivity is observed to: non-immunoglobulin mouse serum proteins serum proteins from bovine, horse, human, pig, or rabbit.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Rabbit anti-mouse IgG (H&L) - DyLight 550 Conjugate, min. cross-reactivity to human serum is a secondary antibody conjugated to DyLight 550, which binds to all mouse IgG (H&L) in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase mouse IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG light chains on all mouse immunoglobulins.No reactivity is observed to: non-immunoglobulin mouse serum proteins human serum proteins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified rabbit IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Donkey anti-Rabbit IgG (H&L) - Affinity Pure, DyLight 550 Conjugate is a secondary antibody conjugated to DyLight 550, which binds to all rabbit IgG (H&L) in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase rabbit IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG light chains on all rabbit immunoglobulins.No reactivity is observed to: non-immunoglobulin rabbit serum proteins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Donkey anti-Rabbit IgG (H&L) - DyLight 550 Conjugated, min. cross-reactivity to human IgG and serum proteins is a secondary antibody conjugated to DyLight 550, which binds to all rabbit IgG (H&L) in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase rabbit IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG light chains on all rabbit immunoglobulins.No reactivity is observed to: non-immunoglobulin rabbit serum proteins mouse IgG or serum proteins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Donkey anti-Rabbit IgG (H&L) - DyLight 550 Conjugate, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human,mouse, rat or sheep IgG is a secondary antibody conjugated to DyLight 550, which binds to all rabbit IgG (H&L) in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase rabbit IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG light chains on all rabbit immunoglobulins.No reactivity is observed to: non-immunoglobulin rabbit serum proteins IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat or sheep.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Goat anti-rabbit IgG (H&L) - F(ab)'2 fragment, DyLight 550 Conjugate, min. cross-reactivity to bovine, human, or mouse lgG or serum proteins is a secondary antibody conjugated to DyLight 550, which binds to all rabbit IgG (H&L), F(ab)'2 fragment in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase rabbit IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0,5 mg of antibody in 0,55 ml of sterile water add 0,55 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG light chains on all rabbit immunoglobulins.No reactivity is observed to: non-immunoglobulin rabbit serum proteins IgG from bovine, human or mouse serum proteins from bovine, human or mouse.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Selected references:
Provost et al. (2019). Sci Rep. 2019 Nov 29;9(1):17967. doi: 10.1038/s41598-019-53528-0.
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Goat anti-human IgG (H&L) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgG (H&L) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Human IgG (H&L)
Expected Species:
Human IgG (H&L)
Immunogen:
Purified human IgG
Applications:
ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
This antibody reacts with alpha (heavy chains) on human IgG and all light chains on human immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin human serum proteins based in immunoelectrophoresis.
No confirmed exceptions from predicted reactivity are currently known
Special application note:
ALP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
Sheep anti-rabbit IgG (H&L) is a secondary antibody conjugated to FITC, which binds to rabbit IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rabbit IgG (H&L). Fluorescein-5-isothiocyanate (FITC) has Amax = 494 nm; Emax = 518 nm.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified sheep IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.5 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rabbit IgG, light chains on all rabbit immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rabbit serum proteins.
Rabbit anti-rat IgG (H&L) is a secondary antibody which binds to rat IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rat IgG (H&L).
This antibody is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified rabbit IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is > 4.5 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rat IgG, light chains on all rat immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rat serum proteins, human serum proteins
Rabbit anti-rat IgG (H&L) is a secondary antibody conjugated to HRP, which binds to rat IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rat IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified rabbit IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Selected references:
Quian et al. (2015). Bone Marrow-Derived Mesenchymal Stem Cells Repair Necrotic Pancreatic Tissue and Promote Angiogenesis by Secreting Cellular Growth Factors Involved in the SDF-1α/CXCR4 Axis in Rats. Stem Cells International Volume 2015 (2015), Article ID 306836.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml. Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.1 % (v/v) Kathon CG as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rat IgG, light chains on all rat immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rat serum proteins.
Rabbit anti-rat IgG (H&L) is a secondary antibody conjugated to FITC, which binds to anti-rat IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rat IgG (H&L). Fluorescein-5-isothiocyanate (FITC) has Amax = 494 nm; Emax = 518 nm
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified rabbit IgG.
Selected references:
Kolbert et al. (2018). Nitro-oxidative stress correlates with Se tolerance of Astragalus species. Plant Cell Physiol. 2018 May 25. doi: 10.1093/pcp/pcy099.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rat IgG, light chains on all rat immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rat serum proteins
Rabbit anti-rat IgG (H&L) is a secondary antibody conjugated to ALP, which binds to rat IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rat IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 500-1 : 2000 (IHC), 1 : 50 000-1 : 5 000 (WB)
Purity:
Immunogen affinity purified rabbit IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.50 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 30 mM triethanolamine, pH 7.2.5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rat IgG, light chains on all rat immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rat serum proteins.
This antibody reacts with the heavy chains on human IgG and with the light chains on all human immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Biotin conjugate is supplied in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, Contains 0,05 % (w/v) sodium azide as preservative
Chicken anti-Rat IgG (H&L) - DyLight 550 Conjugate, min. cross-reactivity to human or rabbit IgG or serum proteins is a secondary antibody conjugated to DyLight 550, which binds to all rat IgG (H&L) in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase rat IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0,5 mg of antibody in 0,55 ml of sterile water add 0,55 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rat IgG light chains on all rat immunoglobulins.No reactivity is observed to: non-immunoglobulin rat serum proteins IgG from human or rabbit serum proteins from human or rabbit.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified chicken IgG.
Reconstitution:
For reconstitution add 0.55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
This antibody reacts with the heavy chains on human IgG and with the light chains on all human immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin human serum or bovine, mouse or rabbit serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of Sodium azide as preservative.Antibody concentration is 4.5 mg/ml.
Goat anti-human IgG (H&L) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgG (H&L) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Human IgG (H&L)
Expected Species:
Human IgG (H&L)
Immunogen:
Purified human IgG
Applications:
ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
This antibody reacts with the heavy (gamma) chains on human IgG and all lights chains of human immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin human serum proteins and serum proteins from mouse, rabbit or bovine based on immunoelectrophoresis.
No confirmed exceptions from predicted reactivity are currently known
Special application note:
ALP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
Donkey anti-Rat IgG (H&L) - Affinity Pure, DyLight 550 Conjugate is a secondary antibody conjugated to DyLight 550, which binds to all rat IgG (H&L) in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase rat IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rat IgG light chains on all rat immunoglobulins.No reactivity is observed to: non-immunoglobulin rat serum proteins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Donkey anti-Rat IgG (H&L) - DyLight 550 Conjugated, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human,mouse, rat or sheep IgG is a secondary antibody conjugated to DyLight 550, which binds to all rat IgG (H&L) in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase rat IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rat IgG light chains on all rat immunoglobulins.No reactivity is observed to: non-immunoglobulin rat serum proteins IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit or sheep.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
This antibody reacts with the heavy chains on human IgG and with the light chains on all human immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin human serum proteins or serum from bovine, mouse, rabbit based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Biotin conjugate is supplied in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, Contains 0,05 % (w/v) sodium azide as preservative
Goat anti-human IgG (H&L) is a secondary antibody conjugated to HRP which binds to human IgG (H&L) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Human IgG (H&L)
Expected Species:
Human IgG (H&L)
Immunogen:
Purified human IgG
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
This antibody reacts with the heavy chains on human IgG and with the light chains on all human immunglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin human serum proteins or bovine, mouse or rabbit serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase
Purified guinea pig IgG contains Protein A purified guinea pig IgG from normal serum, e.g. serum of non immunized animals and is excellent for use as blocking reagent in immunoassays.
Donkey anti-Sheep IgG (H&L) - DyLight 550 Conjugated, min. cross reactivity to human or rabbit IgG is a secondary antibody conjugated to DyLight 550, which binds to all Sheep IgG (H&L) in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase Sheep IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on sheep IgG light chains on all sheep immunoglobulins.No reactivity is observed to: non-immunoglobulin sheep serum proteins IgG from human or rabbit.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Donkey anti-Goat IgG (H&L) , DyLight 594 Conjugate is a secondary antibody conjugated to DyLight 594, which binds to all goat IgGs in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase goat IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy chains on goat IgG light chains on all goat immunoglobulins.No reactivity is observed to: non-immunoglobulin goat serum proteins.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Selected references:
Ainla et al. (2013). Lab on a Biomembrane: rapid prototyping and manipulation of 2D fluidic lipid bilayers circuits. Sci Rep. 2013 Sep 25;3:2743. doi: 10.1038/srep02743.
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Donkey anti-Goat IgG (H&L) , DyLight 594 Conjugated, min. cross-reactivity to human, mouse, rabbit or rat IgG is a secondary antibody conjugated to DyLight 594, which binds to all goat IgGs in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase goat IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on goat IgG light chains on all goat immunoglobulins.No reactivity is observed to: non-immunoglobulin goat serum proteins IgG/serum from human, mouse, rabbit or rat.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Rabbit anti-goat IgG (H&L) , DyLight 594 Conjugate is a secondary antibody conjugated to DyLight 594, which binds to all goat IgGs in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase goat IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on goat IgG light chains on all goat immunoglobulins.No reactivity is observed to: non-immunoglobulin goat serum proteins.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified rabbit IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Goat anti-guinea pig IgG (H&L) , DyLight 594 Conjugate is a secondary antibody conjugated to DyLight 594, which binds to all guinea pig IgG in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase guinea pig IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on guinea pig IgG light chains on all guinea pig immunoglobulins.No reactivity is observed to: non-immunoglobulin guinea pig serum proteins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Goat anti-guinea pig IgG (H&L) , DyLight 594 Conjugated, min. cross reactivity to bovine, chicken, goat, hamster, horse, human, mouse, rabbit, rat, sheep Serum is a secondary antibody conjugated to DyLight 594, which binds to all guinea pig IgG in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase guinea pig IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on guinea pig IgG light chains on all guinea pig immunoglobulins.No reactivity is observed to: non-immunoglobulin guinea pig serum proteins serum proteins from bovine, chicken, goat, hamster, horse, human, mouse, rabbit, rat or sheep.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Goat anti-human IgG Fc , DyLight 594 Conjugate is a secondary antibody conjugated to DyLight 594, which binds to all human IgG Fc in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase human IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on human IgG.No reactivity is observed to: non-immunoglobulin human serum proteins light chains on all human immunoglobulins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Goat anti-human IgM ( chain) , DyLight 594 Conjugate is a secondary antibody conjugated to DyLight 594, which binds to all human IgM ( chain) in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase human IgM.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy ( ) chains on human IgM.No reactivity is observed to: non-immunoglobulin human serum proteins light chains on all human immunoglobulins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Donkey anti-Rabbit IgG (H&L) - Affinity Pure, DyLight 633 Conjugate, min x w/bovine, chicken, goat, guinea pig, hamster, horse, human,mouse, rat or sheep IgG is a secondary antibody conjugated to DyLight 633, which binds to rabbit IgG (H&L) in immunological assays.DyLight 633 has Amax = 638 nm, Emax = 658 nm. Antibodies are affinity purified using solid phase rabbit IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
immunolocalization Donkey anti-rabbit conjugated to DY Light 633 in the absence of primary antibody show very little background label (right image) and binds to primary antibodies (anti-Arf1 and anti-Sec21p; two left images). The top images show only the antibody label in red and bottom images show overlay of antibody label in red with DAPI in blue and DIC/Nomarski image in grey. The material has been prepared using whole mount immunolabelling procedure of 5 day old Arabidopsis thaliana seedlings (Fischer, U. et al. (2006). Curr. Biol. 16, 2143–2149). Antibody dilutions: rabbit anti-sec21p (Agrisera, AS08 327) 1:1000, rabbit anti-Arf1 (Agrisera, AS08 325) 1:1000 and Agrisera Donkey anti-rabbit DY Light 633 1:100. DNA was stained with DAPI. Courtesy: Dr. Anna Gustavsson and Prof. Markus Grebe, Ume Plant Science Centre
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, no reactivity is observed to:non-immunoglobulin rabbit serum proteinsIgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat.Based on immunoelectrophoresis, this antibody reacts with: (γ) chains on rabbit IgG light chains on all rabbit immunoglobulins.
Rabbit anti-human IgG (H&L) is a secondary antibody which binds to human IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase human IgG (H&L).
This antibody is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified rabbit IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is > 4.5 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on human IgG, light chains on all human immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin human serum proteins.
Rabbit anti-human IgG (H&L) , DyLight 594 Conjugate is a secondary antibody conjugated to DyLight 594, which binds to all human IgG in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase human IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on human IgG light chains on all human immunoglobulins.No reactivity is observed to: non-immunoglobulin human serum proteins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified rabbit IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Donkey anti-Mouse IgG (H&L) , DyLight 594 Conjugate is a secondary antibody conjugated to DyLight 594, which binds to all mouse IgG (H&L) in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase mouse IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG light chains on all mouse immunoglobulins.No reactivity is observed to: non-immunoglobulin mouse serum proteins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Donkey anti-Mouse IgG (H&L) , DyLight 594 Conjugated, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep IgG is a secondary antibody conjugated to DyLight 594, which binds to all mouse IgG (H&L) in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase mouse IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG light chains on all mouse immunoglobulins.No reactivity is observed to: non-immunoglobulin mouse serum proteins IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Rabbit anti-chicken IgG/Y (H&L) is a secondary antibody conjugated to TRITC , which binds to chicken IgG/Y (H&L) in immunological assays. Antibody is affinity purified using solid phase chicken IgG/Y. Tetramethylrhodamine-5-isothiocyanate (TRITC) has Amax = 550 nm; Emax = 570 nm.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified rabbit IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on chicken IgG (IgY), light chains on all chicken immunoglobulins Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin chicken serum proteins
Goat anti-mouse IgG (H&L) , DyLight 594 Conjugated, min. cross-reactivity to bovine, horse, human, pig or rabbit serum proteins is a secondary antibody conjugated to DyLight 594, which binds to all mouse IgG (H&L) in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase mouse IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG light chains on all mouse immunoglobulins.No reactivity is observed to: non-immunoglobulin mouse serum proteins serum proteins from bovine, horse, human, pig, or rabbit.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Donkey anti-Rabbit IgG (H&L) , DyLight 594 Conjugate is a secondary antibody conjugated to DyLight 594, which binds to all rabbit IgG (H&L) in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase rabbit IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG light chains on all rabbit immunoglobulins.No reactivity is observed to: non-immunoglobulin rabbit serum proteins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Donkey anti-Rabbit IgG (H&L) , DyLight 594 Conjugated, min. cross-reactivity to human IgG and serum proteins is a secondary antibody conjugated to DyLight 594, which binds to all rabbit IgG (H&L) in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase rabbit IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG light chains on all rabbit immunoglobulins.No reactivity is observed to: non-immunoglobulin rabbit serum proteins mouse IgG or serum proteins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Donkey anti-Rabbit IgG (H&L) , DyLight 594 Conjugated, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human,mouse, rat or sheep IgG is a secondary antibody conjugated to DyLight 594, which binds to all rabbit IgG (H&L) in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase rabbit IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG light chains on all rabbit immunoglobulins.No reactivity is observed to: non-immunoglobulin rabbit serum proteins IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat or sheep.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Rabbit anti-chicken IgY (H&L) is a secondary antibody conjugated to HRP, which binds to in immunological assays. Antibody is affinity purified using solid phase chicken IgY.Chicken egg yolk immunoglobulin (IgY) has been also called chicken IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Host Animal:
Rabbit
Species Reactivity:
Heavy chains on chicken IgY and light chains on all chicken immunoglobulins,
Immunogen:
Purified Chicken IgY (H&L), whole molecule
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified rabbit IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily
Not reactive in:
Non-immunoglobulin chicken serum proteins
Selected references:
Li et al. (2022), The effects of Ni availability on H2 production and N2 fixation in a model unicellular diazotroph: The expression of hydrogenase and nitrogenase. Limnol Oceanogr, 67: 1566-1576. https://doi.org/10.1002/lno.12151Panayiotou et al. (2018). Viperin restricts Zika virus and tick-borne encephalitis virus replication by targeting NS3 for proteasomal degradation.J Virol. 2018 Jan 10. pii: JVI.02054-17. doi: 10.1128/JVI.02054-17.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml. Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.1 % (v/v) Kathon CG as preservative of bacterial growth.
Rabbit anti-chicken IgG/Y (H&L) is a secondary antibody conjugated to FITC, which binds to chicken IgG/Y (H&L) in immunological assays. Antibody is affinity purified using solid phase chicken IgG/Y. Fluorescein-5-isothiocyanate (FITC) has Amax = 494 nm; Emax = 518 nm.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified rabbit IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on chicken IgG (IgY), light chains on all chicken immunoglobulins Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin chicken serum proteins
Based on IEP this antibody reacts with: F(ab')2 fragment of human IgG. Based on IEP no reactivity is observed to: non-immunoglobulin human serum proteinsFc fragment of human IgG
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of sodium azide as preservative. It is a clear, colourless liquid, filter sterilized.Antibody purity ≥95% based on SDS-PAGE.
Rabbit anti-chicken IgG/Y (H&L) is a secondary antibody which binds to chicken IgG/Y (H&L) in immunological assays. Antibody is affinity purified using solid phase chicken IgG/Y.
This antibody is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified rabbit IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is > 4.5 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on chicken IgG (IgY), light chains on all chicken immunoglobulins Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin chicken serum proteins
Based on IEP this antibody reacts with: F(ab')2 fragment of human IgG. Based on IEP no reactivity is observed to: Fc fragment of human IgGnon-immunoglobulin human serum proteinsserum proteins from bovine, horse and mouse
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of sodium azide as preservative. It is a clear, colourless liquid, filter sterilized.Antibody purity ≥95% based on SDS-PAGE.
Purified mouse IgG contains Protein A purified mouse IgG from normal serum, e.g. serum of non immunized animals and is excellent for use as blocking reagent in immunoassays.
Total IgG. Protein A purified in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2. Contains 0.05 % sodium azide.
Special application note:
Mouse IgG is provided in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. 0.05 % sodium azide is added as preservative.This immunoglobulin preparation can be used as a negative control for immunoprecipitation (IP).Antibody will react with all IgG subclasses.
This antibody reacts with the heavy chains on human IgG and with the light chains on all human immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of Sodium azide as preservative.Antibody concentration is 3 mg/ml.Antibody purity ≥90% based on SDS-PAGE. May contain small amounts of intact IgG.
Goat anti-human IgG (H&L), F(ab)'2 fragment is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgG (H&L) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Human IgG (H&L)
Expected Species:
Human IgG (H&L)
Immunogen:
Purified human IgG
Applications:
ELISA (ELISA) , Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
This antibody reacts with the alpha chains on human IgG based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.
No confirmed exceptions from predicted reactivity are currently known
Special application note:
ALP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative.Purity is ≥ 90% based on SDS-PAGE. May contain small amounts of intact IgG.
This antibdy reacts with the heavy chains on human IgG and with the light chains on all human immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Goat anti-rabbit IgG (H&L) is a secondary antibody conjugated to FITC, which binds to rabbit IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rabbit IgG (H&L). Fluorescein-5-isothiocyanate (FITC) has Amax = 494 nm; Emax = 518 nm
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rabbit IgG, light chains on all rabbit immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rabbit serum proteins serum proteins from human, mouse or rat
Goat anti-human IgG (H&L), F(ab)'2 fragment is a secondary antibody conjugated to HRP which binds to human IgG (H&L), F(ab)'2 fragment in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Human IgG (H&L), F(ab)'2 fragment
Expected Species:
Human IgG (H&L), F(ab)'2 fragment
Immunogen:
purified human IgG (H&L)
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
This antibody reacts with the heavy chains on human IgG and with the light chains on all human immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 0,55 ml of sterile water
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidasePurity is ≥ 90% based on SDS-PAGE. May contain small amounts of intact IgG.
Goat anti-rabbit IgG (H&L) is a secondary antibody conjugated to biotin, which binds to rabbit IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rabbit IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rabbit IgG, light chains on all rabbit immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rabbit serum proteins, serum proteins from human, mouse or rat.
Goat anti-human IgG (H&L), F(ab)'2 fragment is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgG (H&L), F(ab)'2 fragment in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Human IgG (H&L), F(ab)'2 fragment
Expected Species:
Human IgG (H&L), F(ab)'2 fragment
Immunogen:
Purified human IgG (H&L)
Applications:
ELISA (ELISA) , Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
This antibody reacts with the heavy (gamma) chains on human IgG and light chains on all human immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin human serum proteins and serum from mouse, rabbit of bovine serum proteins based on immunoelectrophoresis.
No confirmed exceptions from predicted reactivity are currently known
Special application note:
ALP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative.Purity ≥90% based on SDS-PAGE. May contain small amounts of intact IgG.
Goat anti-rabbit IgG (H&L) is a secondary antibody conjugated to biotin, which binds to rabbit IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rabbit IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rabbit IgG, light chains on all rabbit immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rabbit serum proteins, human serum proteins.
Goat anti-rabbit IgG (H&L) is a secondary antibody conjugated to ALP, which binds to rabbit IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rabbit IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE, Antibody concentration is 1,50 mg/ml (E 1% at 280 nm = 13,0), Antibody is supplied in 30 mM triethanolamine, pH 7,2,5 mM magnesium chloride, 0,1 mM zinc chloride, 1 % (w/v) B, Protease/IgG free, Contains 0,05% (w/v) sodium azide as preservative of bacterial growth, Based on immunoelectrophoresis,this antibody reacts with: heavy chains on rabbit IgG, light chains on all rabbit immunoglobulins, Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rabbit serum proteins, human serum proteins
Goat anti-biotin is a secondary antibody conjugated to TRITC, which binds to biotin in immunological assays. Antibody is affinity purified using solid phase biotin. Tetramethylrhodamine-5-isothiocyanate (TRITC) has Amax = 550 nm; Emax = 570 nm
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE, Antibody concentration is 1,0 mg/ml (E 1% at 280 nm = 13,0), Antibody is supplied in 10 mM sodium phosphate, 0,15 M sodium chloride, pH 7,2,1 % (w/v) B, Protease/IgG free, Contains 0,05% (w/v) sodium azide as preservative of bacterial growth
Goat anti-human IgG Fc (two heavy chains with constant domains) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgG Fc (two heavy chains with constant domains) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Human IgG Fc (two heavy chains with constant domains)
Expected Species:
Human IgG Fc (two Heavy chains with constant domains)
Immunogen:
Purified human IgG
Applications:
ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.No reactivity is observed with the light chains on human immunoglobulins and non-immunoglobulin human serum proteins and human IgG F(ab)'2 fragment based in immunoelectrophoresis.
No confirmed exceptions from predicted reactivity are currently known
Special application note:
ALP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
Goat anti-biotin is a secondary antibody conjugated to HRP, which binds to biotin in immunological assays. Antibody is affinity purified using solid phase biotin.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE, Antibody concentration is 1,0 mg/ml, Antibody is supplied in 10 mM sodium phosphate, 0,15 M sodium chloride, pH 7,2,1 % (w/v) B, Protease/IgG free, Contains 0,1 % (v/v) Kathon CG as preservative of bacterial growth
Goat anti-biotin is a secondary antibody conjugated to FITC, which binds to biotin in immunological assays. Antibody is affinity purified using solid phase biotin. Fluorescein-5-isothiocyanate (FITC) has Amax = 494 nm; Emax = 518 nm.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE, Antibody concentration is 1,0 mg/ml (E 1% at 280 nm = 13,0), Antibody is supplied in 10 mM sodium phosphate, 0,15 M sodium chloride, pH 7,2,1 % (w/v) B, Protease/IgG free, Contains 0,05% (w/v) sodium azide as preservative of bacterial growth
Goat anti-human IgG Fc (two heavy chains with constant domains) is a secondary antibody conjugated to biotin which binds to human IgG Fc (two heavy chains with constant domains) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store non-diluted antibody at 2-8°C. Shelf life is 1 year from date of receipt.
Host Animal:
Goat
Species Reactivity:
Human IgG Fc (two heavy chains with constant domains)
Expected Species:
Human IgG Fc (two Heavy chains with constant domains)
This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.No reactivity is observed to immunoglobulin light chains, non-immunoglobulin human serum proteins or IgG F(ab)'2 based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Biotin conjugate is supplied in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, Contains 0,05 % (w/v) sodium azide as preservative
Donkey anti-Goat IgG (H&L), DyLight 488 Conjugated is a secondary antibody conjugated to DyLight 488, which binds to Goat IgG (H&L) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Goat IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with:, heavy chains on goat IgG, light chains on all goat immunoglobulinsNo reactivity is observed to, non-immunoglobulin goat serum proteinsBSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Goat anti-human IgG Fc (two heavy chains with constant domains) is a secondary antibody conjugated to HRP which binds to human IgG Fc (two heavy chains with constant domains) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Human IgG Fc (two heavy chains with constant domains)
Expected Species:
Human IgG Fc (two Heavy chains with constant domains)
Immunogen:
Purified human IgG
Applications:
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.No reactivity is observed with the light chains on human immunglobulins or non-immunoglobulin human serum proteins or bovine, mouse or rabbit serum proteins or IgG F(ab)' fragment based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase
This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.No reactivity is observed with the light chains on human immunoglobulins and non-immunoglobulin human serum proteins and human IgG F(ab)'2 fragment based in immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Biotin conjugate is supplied in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, Contains 0,05 % (w/v) sodium azide as preservative,Purity is ‰ 90% based on SDS-PAGE, May contain small amounts of intact IgG
Goat anti-llama IgG (H&L) is a secondary antibody which binds to llama immunoglobulins (H&L) in immunological assays. Antibody is affinity purified using solid phase Llama IgG (H&L).
This antibody is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on llama IgG, light chains on all llama immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin llama serum proteins.This antibody will react with VHH of llama IgG's.
Antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified chicken IgY.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody is supplied in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 0,05 % (w/v) of Sodium azide as preservative
Antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified rabbit IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody is supplied in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 0,05 % (w/v) of Sodium azide as preservative
Antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified chicken IgY.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of Sodium azide as preservative.Antibody concentration is 4.5 mg/ml.
Chicken anti-rabbit IgG (H&L) is a secondary antibody conjugated to HRP which binds to rabbit IgG (H&L) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store non-diluted antibody at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Chicken
Species Reactivity:
Rabbit IgG (H&L)
Expected Species:
Rabbit IgG (H&L)
Immunogen:
Purified Rabbit IgG
Applications:
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified chicken IgY.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Levitan et al. (2019). Structural and functional analyses of photosystem II in the marine diatom Phaeodactylum tricornutum. Proc Natl Acad Sci U S A. 2019 Aug 27;116(35):17316-17322. doi: 10.1073/pnas.1906726116.Gao et al. (2018). Cisgenic overexpression of cytosolic glutamine synthetase improves nitrogen utilization efficiency in barley and prevents grain protein decline under elevated CO2. Plant Biotech. J. 10.1111/pbi.13046
Special application note:
Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.2)HRP-conjugate is supplied in PBS, 1% BSA and 0.1% proclin 150.0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase
Chicken anti-rabbit IgG (H&L)is a secondary antibody which binds to rabbit IgG (H&L) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store non-diluted antibody at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum and human or mouse serum or IgG based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified chicken IgY.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of Sodium azide as preservative.Antibody concentration is 4.5 mg/ml.
Chicken anti-rabbit IgG (H&L) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to rabbit IgG (H&L) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Chicken
Species Reactivity:
Rabbit IgG (H&L)
Expected Species:
Rabbit IgG (H&L)
Immunogen:
Purified Rabbit IgG
Applications:
ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
This antibody reacts with the heavy chains on rabbit IgG and the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins or to human or mouse serum or IgG based on immunoelectrophoresis.
No confirmed exceptions from predicted reactivity are currently known
Special application note:
ALP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
This antibody reacts with the heavy chains on rabbit IgG and the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum or human and mouse serum or IgG based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified chicken IgY.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Biotin conjugate is supplied in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, Contains 0,05 % (w/v) sodium azide as preservative
Chicken anti-rabbit IgG (H&L) is a secondary antibody conjugated to HRP which binds to rabbit IgG (H&L) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Chicken
Species Reactivity:
Rabbit IgG (H&L)
Expected Species:
Rabbit IgG (H&L)
Immunogen:
Purified Rabbit IgG
Applications:
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins and human and mouse serum and IgG on immunoelectrophoresis.This antibody was absorbed against solid phase human and mouse serum and IgG.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified chicken IgY.
Reconstitution:
For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified donkey IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Biotin conjugate is supplied in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, Contains 0,05 % (w/v) sodium azide as preservative
Donkey anti-Rabbit IgG (H&L), DyLight 488 Conjugated is a secondary antibody conjugated to DyLight 488, which binds to Rabbit IgG (H&L) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Rabbit IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG, light chains on all rabbit immunoglobulinsNo reactivity is observed to: non-immunoglobulin rabbit serum proteins
Donkey anti-rabbit IgG (H&L) is a secondary antibody conjugated to HRP which binds to rabbit IgG (H&L) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store non-diluted antibody at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Donkey
Species Reactivity:
Rabbit IgG (H&L)
Expected Species:
Rabbit IgG (H&L)
Immunogen:
Purified Rabbit IgG
Applications:
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified donkey IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase
Goat anti-rabbit IgG (H&L), F(ab)'2 fragment is a secondary antibody which binds to rabbit IgG in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of Sodium azide as preservative.Purity is ≥ 90 % based on SDS-PAGE. May contain small amounts of intact IgG.
Purified sheep IgG contains Protein G purified sheep IgG from normal serum, e.g. serum of non immunized animals and is excellent for use as blocking reagent in immunoassays.
This antibdy reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Biotin conjugate is supplied in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, Contains 0,05 % (w/v) sodium azide as preservative,Purity is ‰ 90% based on SDS-PAGE, May contain small amounts of intact IgG
Goat anti-rabbit IgG (H&L), F(ab)'2 fragment is a secondary antibody conjugated to HRP which binds to rabbit IgG (H&L) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Rabbit IgG (H&L)
Expected Species:
Rabbit IgG (H&L)
Immunogen:
Purified Rabbit IgG (H&L)
Applications:
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Linster et al. (2015). Downregulation of N-terminal acetylation triggers ABA-mediated drought responses in Arabidopsis. Nat Commun. 2015 Jul 17;6:7640. doi: 10.1038/ncomms8640.
Special application note:
Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.0)HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidasePurity is ≥ 90% based on SDS-PAGE. May contain small amounts of intact IgG.
Goat anti-rabbit IgG Fc (two heavy chains with constant domains) is a secondary antibody conjugated to biotin which binds to rabbit IgG Fc (two heavy chains with constant domains) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store non-diluted antibody at 2-8°C. Shelf life is 1 year from date of receipt.
Host Animal:
Goat
Species Reactivity:
rabbit IgG Fc (two heavy chains with constant domains)
Expected Species:
Rabbit IgG Fc (two Heavy chains with constant domains)
This antibody reacts with the heavy chains on rabbit IgG based on immunoelectrophoresis.No reactivity is observed to immunoglobulin light chains or non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Biotin conjugate is supplied in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, Contains 0,05 % (w/v) sodium azide as preservative
Goat anti-rabbit IgG (H&L), DyLight 550 conjugated, is a secondary antibody which binds to rabbit IgGs in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Fluor to protein ratio is 2.47 moles DyLight 550 per mole antibody. Antibodies are adsorbed against bovine,goat,human,mouse,rat IgG and are affinity purified using solid phase rabbit IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy (gamma) chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins and bovine,goat,human,mouse,rat IgG based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
ABF3 (Abscisic acid-insensitive 5-like 6) binds to the ABA-responsive element (ABRE) and mediates stress-responsive ABA signaling.Alternative names: Abscisic acid responsive elements-binding factor 3, ABRE-binding factor 3, Dc3 promoter-binding factor 5, AtDPBF5, bZIP transcription factor 37, AtbZIP37
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water
Special application note:
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Rabbit IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.1% (v/v) Kathon CG is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on rabbit IgGBased on IEP, no reactivity is observed to: • non-immunoglobulin rabbit serum proteins • light chains on all rabbit immunoglobulins • Rabbit IgG F(ab)'2 fragment • serum proteins from bovine, horse or human
The Plus HRP One-Step Polymer anti-Mouse/Rabbit is designed for the qualitative detection of antigens in fixed paraffin-embedded tissue sections, in frozen tissue sections, and in cytological samples. It was developed for use in combination with mono- and polyclonal primary antibodies and sera obtained from mice or rabbit. The kit can be used for examining tissues fixed in different solutions, e.g. formalin (neutrally buffered), B5, Bouin, ethanol, or HOPE.
The purpose of the immunohistochemical staining is to make tissue and cell antigens visible. The Plus HRP One-Step Polymer anti-Mouse/Rabbit is a highly sensitive detection reagent intended for use in immunohistochemistry and immunocytochemistry. The enzyme polymer consists of several molecules of secondary antibodies covalently bound to several molecules of horseradish peroxidase (HRP). Visualisation occurs via an enzyme-substrate reaction in the presence of a colorising reagent which permits microscopical analysis. The test system is suitable for the detection of mono- and polyclonal primary antibodies and sera obtained from mice or rabbit. In contrast to other detection techniques, which often use the streptavidin-biotin system the Plus HRP One-Step Polymer kits avoid the problem of background staining caused by endogenous biotin in the tissue.
Principle of method:
Paraffin-embedded tissue sections are first deparaffinised and rehydrated. Endogenous peroxidase activity in the tissue may cause non-specific staining. This enzyme activity can be blocked by incubation with 3% H2O2-solution (peroxide block). Background staining caused by unspecific binding of the primary antibody or the secondary antibody in the HRP One-Step Polymer is minimized by incubation with a protein blocking solution. This step can be omitted if the primary antibodies are diluted in an appropriate buffer. The next step is incubation with the specific primary antibody. After washing, the HRP One-Step Polymer is applied and incubated. Any excess of unbound polymer is thoroughly washed away after incubation. The addition of the chromogenic substrate starts the enzymatic reaction of the peroxidase which leads to colour precipitation where the primary antibody is bound. The colour can be observed with a light microscope. The chromogen used determines the colour. The chromogen AEC leads to the formation of a red-brown product of reaction at the place of the target antigen. The chromogen DAB forms a dark brown precipitate.
Reagents provided:
6 mL HRP One-Step-Polymer anti-Mouse/Rabbit (ready-to-use) Substrate systems recommended: Permanent AEC kit, AEC single solution, AEC substrate kit, DAB substrate kit, DAB High contrast kit. Materials required but not supplied: Positive and negative control tissue Xylene or suitable substitutes Ethanol, distilled H2O 3% H2O2 solution Reagents for enzyme digestion or heat pre-treatment Wash buffer PBS or TBS Pink PAP Pen Primary antibody (user-defined) Primary antibody diluent Negative control reagent Chromogenic substrate Counter stain solution Mounting medium Cover slips
Storage and handling:
The solution should be stored at 2-8°C without further dilution. Please store the reagent in a dark place and do not freeze it. Under these conditions the solution is stable up to the expiry date. It should not be used after the expiry date. A positive and a negative control have to be carried out in parallel to the test material. If you observe unusual staining or other deviations from the expected results which could possibly be caused by the kit reagents, please contact our technical support .
Reagent preparation:
Reagents should be at room temperature when used. Deparaffinise and rehydrate paraffin-embedded tissue sections. Pre-treatment (optional) with HIER (Heat Induced Epitope Retrieval) or enzymatic digestion. Tissue sections have to be completely covered with the different reagents in order to avoid drying out.
Procedure:
1. Peroxide blocking (3 % H2O2 solution) 10 min. 2. Washing with wash buffer 1 x 2 min. 3. Blocking Solution (This step is optional.) 5 min. 4. Washing with wash buffer 1 x 2 min. 5. Primary antibody (optimally diluted) or negative control reagent 30-60 min. 6. Washing with wash buffer 3 x 5 min. 7. HRP One-Step Polymer anti-Mouse/Rabbit 30 min. 8. Washing with wash buffer 3 x 2 min. 9. AEC or DAB (Controlling the colour intensity via light microscope is recommended.) 5-15 min. 10. Stopping the reaction with distilled H2O when the desired colour intensity is attained 11. Counterstaining and blueing 12. Mounting: aqueous with AEC, permanent with DAB
Expected results:
During the reaction of the substrate with horseradish peroxidase in the presence of a chromogen, a coloured precipitate is formed at the location of the bound primary antibody. This reaction only takes place if the target antigen is existent in the tissue. The chromogen used determines the colour of the precipitate. The analysis is carried out using a light microscope
Trouble shooting:
If you observe unusual staining or other deviations from the expected results which could possibly be caused by the reagents, please read these instructions carefully, contact our technical support. No staining on an actually positive control slide: 1. Reagents were not used in the proper order. 2. Chromogenic substrate solution was too old. 3. Bleaching because chromogen and mounting medium are incompatible. 4. The antigen/epitope in the tissue was insufficiently accessible to the primary antibody. Try a pre-treatment such as heat pre-treatment or enzyme digestion. If you used a pre-treatment it should be extended. 5. Primary antibody not from mouse or rabbit but from a different species. 6. The antigen/epitope was not stable in the fixation and/or pre-treatment procedure used. Try another fixation or pre-treatment. Weak staining: 1. Inadequate fixation or overfixation. 2. Incomplete deparaffinisation. 3. The antigen/epitope in the tissue was insufficiently accessible to the primary antibody. If you used heat pre-treatment or enzyme digestion it should be extended. 4. Excessive incubation with Blocking Solution or insufficient washing after this step. 5. Too much wash buffer remains on the slides after washing, diluting the reagents applied in the next step. 6. Incubation times were too short or primary antibody concentration too low. 7. Chromogenic substrate solution was too old. Nonspecific background staining or overstaining: 1. Incomplete deparaffinisation. 2. Excessive tissue adhesive on slides. 3. Insufficient washing especially after the incubation with the enzyme polymer or the chromogenic substrate solution. These washings are critical. 4. Tissue was allowed to (partially) dry out with reagents on. 5. Unspecific binding of the primary antibody. Please use Blocking Solution or dilute the primary antibody in appropriate diluents. 6. Incubation time of the primary antibody was too long or primary antibody concentration too high. 7. Incubation time of the chromogenic substrate solution was too long or reaction temperature too high (e.g. if temperature in the laboratory is high). 8. The substrate is metabolised by endogenous horse radish peroxidase in the tissue. Maybe the hydrogen peroxide solution used for blocking was inactivated
Quality Control:
We recommend carrying out a positive and a negative control with every staining run. The positive control permits the validation of appropriate processing of the sample. If the negative control has a positive result, this points to unspecific staining.
Performance characteristics:
Studies have been conducted to evaluate the performance of the kit reagents. The product has been found to be suitable for the intended use
Limitations of procedure:
Immunohistochemistry is a complex method in which histological as well as immunological detection methods are combined. Tissue processing and handling prior to immunostaining, for example variations in fixation and embedding or the inherent nature of the tissue can cause inconsistent results (Nadji and Morales, 1983). Endogenous peroxidase or pseudoperoxidase activity may cause non-specific staining. The enzyme activity is blocked by incubation with hydrogen peroxide solution. Tissues containing Hepatitis B Surface Antigen (HBsAg) may give false positive results with HRP (horseradish peroxidase) detection systems (Omata et al, 1980). Inadequate counterstaining and mounting can influence the interpretation of the results. The colour intensity of the reaction product can decrease with time, especially when exposed to light. Overexposure with the protein blocking solution can result in decreasing signal intensity. Sanbio guarantees that the product will meet all requirements described from its shipping date until its expiry date, as long as the product is correctly stored and utilized. No additional guarantees can be given. Under no circumstances shall Sanbio be liable for any damages arising out of the use of the reagent provided.
Precautions:
Use by qualified personnel only. Wear protective clothing to avoid eye, skin or mucous membrane contact with the reagents. In case of a reagent coming into contact with a sensitive area, wash the area with large amounts of water. Microbial contamination of the reagents must be avoided, since otherwise non-specific staining might appear. ProClin 950 is used for stabilisation. A Material safety data sheet (MSDS) is available upon request.
Mouse anti-Non-specific control IgG Monoclonal Antibody (Unconjugated), suitable for WB, IHC, ICC, FC, ELISA.
Background Info:
Commonly used mouse IgG1 negative control antibody clone derived from a Balb/c myeloma and is recommend as a negative control for a variety of immunohistochemical applications where mouse IgG experimental antibodies are use. To date no published reactivity as been associated with X63.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Host Animal:
Mouse
Species Reactivity:
Non-Reactive (Negative Control)
Immunogen:
None. This control IgG has no known binding ability.
Applications:
ELISA,FC,ICC,IHC,WB
Clone number:
X63
Antibody Isotype:
IgG1, kappa
Application Details:
Recommended for use as a control for Western Blotting, immunohistochemistry and FACS at a concentration equal to that of the test antibody. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
No staining has ever been identified with this immunoglobulin demonstrating its non-specific value as a control. N/A
Storage:
Store lyophilized antibody at 2-8°C. Keep reconstituted antibody at -20°C to -80°C for long-term storage. For short term keep at 2-8°C. We suggest that the customer aliquots the antibody into smaller lots to avoid repeated freezing and thawing.
Purification:
Immunoglobulin purified using Protein G column. Purity analysed using gel electrophoresis.
Goat anti-Human IgG Fc, Biotin conjugated, min. cross-reactivity to human IgA + IgM, bovine, horse, mouse or rabbit serum proteins is a secondary antibody conjugated to biotin, which binds to human IgG Fc in immunological assays.
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Human IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on human IgG .Based on IEP, no reactivity is observed to: • non-immunoglobulin human serum immunoglobulins • light chains on all human immunoglobulins • human IgA or IgM • serum proteins from bovine, mouse, or rabbit .
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Mouse IgM, µ chain
Buffer:
PBS, 1% BSA & 0.1% proclin 150
Preservative:
0.1% (v/v) Kathon CG
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.
Specificity:
Based on IEP, this antibody reacts with: · heavy (µ) chains on mouse IgM
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum proteins · light chains on all mouse immunoglobulins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Host:
Donkey
Immunogen:
Purified Mouse IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum immunoglobulins · IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Host:
Donkey
Immunogen:
Purified Rabbit IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on Rabbit IgG · light chains on all Rabbit immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin Rabbit serum immunoglobulins · IgG from Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat or Sheep
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Donkey
Immunogen:
Purified Sheep IgG, whole molecule
Buffer:
PBS, 1% BSA & 0.1% proclin 150
Preservative:
0.1% (v/v) Kathon CG
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on sheep IgG · light chains on all sheep immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin sheep serum proteins · IgG from human or rabbit · serum proteins from human or rabbit
Country Of Origin:
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Immunoglobulin M (IgM) is produced as a 900 kDa pentamer, which is an efficient complement binder. This antibody type is produced initially in the immune response and it is the first immunoglobulin class to be synthesized by a fetus or newborn. IgM antibodies do not cross the placenta. IgM concentration in blood is 0.12 g/l and its biological survival (plasma T1/2) is 5 days.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
IgM from human serum.
Applications:
ELISA
Additional Info:
The antibody MA2 reacts with µ-chain of human IgM, Fab - region localized epitope.
CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. CD25+ CD4+ FoxP3+ regulatory cells (Treg cells) play a crucial role in the control of organ-specific autoimmune diseases.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
B6.1 CTL cell line
Applications:
FC,IP,IHC,FA
Additional Info:
The rat monoclonal antibody PC61.5 (PC61.5.3) recognizes CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4<sup>+</sup> T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.
CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. CD25+ CD4+ FoxP3+ regulatory cells (Treg cells) play a crucial role in the control of organ-specific autoimmune diseases.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Immunogen:
B6.1 CTL cell line
Applications:
FC,IP,IHC,FA
Additional Info:
The rat monoclonal antibody PC61.5 (PC61.5.3) recognizes CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4<sup>+</sup> T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.
Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75% of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Fusion protein of human IgG Fc fragment.
Applications:
WB,ICC,ELISA,FC
Additional Info:
The mouse monoclonal antibody EM-07 reacts with Fc part of human IgG heavy chain and with isolated Fc fragments.
Clone number:
EM-07
Antibody Isotype:
IgG1
Application Details:
Western blotting and ELISA: The peroxidase conjugate of this antibody is suitable for detection of human IgG Fc fragments. The antibody EM-07 does not crossreact with IgM.
Immunoglobulin M (IgM) is produced as a 900 kDa pentamer, which is an efficient complement binder. This antibody type is produced initially in the immune response and it is the first immunoglobulin class to be synthesized by a fetus or newborn. IgM antibodies do not cross the placenta. IgM concentration in blood is 0.12 g/l and its biological survival (plasma T1/2) is 5 days.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Purified human IgM.
Applications:
WB,ICC,ELISA,FC
Additional Info:
The antibody CH2 reacts with Fc fragment of human IgM.
Clone number:
CH2
Antibody Isotype:
IgG1
Application Details:
Western blotting: Recommended dilution: 1 ?g/ml.Flow cytometry: Recommended dilution: 1-4 ?g/ml, extracellular and intracellular staining.
CD49e, the alpha 5 integrin, noncovalently associates with the beta 1integrin (CD29) to form VLA-5 integrin complex. CD49e itself is composed of two disulfide-linked chains of 135 kDa and 25 kDa. VLA-5 binds to RGD sequence of fibronectin, and to neural adhesion molecule L1. It is important in maintaining the integrity of endothelial monolayers, in monocyte migration into the extracellular tissues, and it also provides a co-stimulatory signal to T cells and enhances receptor and complement receptor-mediated phagocytosis.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
C57BL/6 x A/J)F1 murine mast cell line
Applications:
FC
Additional Info:
The rat monoclonal antibody 5H10-27 (MFR5) recognizes an extracellular epitope of murine CD49e, a 135 kDa protein serving as VLA-5 alpha chain, expressed on thymocytes, activated T cells, splenic B cells, and mast cells.
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ?g/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody PPV-06, generated against a plant pathogen, does not cross-react with other species, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG1 molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Applications:
FC,IP,IHC,ICC,WB,ELISA
Additional Info:
This mouse IgG1 monoclonal antibody (clone PPV-06) reacts with undefined epitope on a plant pathogen.
Clone number:
PPV-06
Antibody Isotype:
IgG1
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ?g/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-21, generated against an undefined antigen, does not react specifically with rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG1 molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Product Type:
Antibodies Isotype Control
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Applications:
FC,IP,WB,IHC,ICC,ELISA
Additional Info:
This mouse IgG1 kappa monoclonal antibody (clone MOPC-21) with unknown specificity has been confirmed as a good negative control with human and rat species, based on multiple testing on rat and human tissues.
Clone number:
MOPC-21
Antibody Isotype:
IgG1 k
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ?g/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-21, generated against an undefined antigen, does not react specifically with rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG1 molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Product Type:
Antibodies Isotype Control
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Applications:
FC,IP,WB,IHC,ICC,ELISA,FA
Additional Info:
This mouse IgG1 kappa monoclonal antibody (clone MOPC-21) with unknown specificity has been confirmed as a good negative control with human and rat species, based on multiple testing on rat and human tissues.
Clone number:
MOPC-21
Antibody Isotype:
IgG1 k
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ?g/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-173, generated against an undefined antigen, does not react specifically with mouse, rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG2a molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Product Type:
Antibodies Isotype Control
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
The transplantable plasmacytoma MOPC-173 was induced by intraperitoneal injection of mineral oils into BALB/c mice.
Applications:
FC,ICC,WB,IP,IHC,ELISA
Additional Info:
This mouse IgG2a monoclonal antibody (clone MOPC-173) reacts with an unknown epitope. It does not react with a variety of resting, activated, live, and fixed mouse, rat and human tissues.
Clone number:
MOPC-173
Antibody Isotype:
IgG2a k
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ?g/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody PPV-04, generated against a plant pathogen, does not cross-react with other species, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG2a molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Applications:
FC,IP,IHC,ICC,WB,ELISA
Additional Info:
This mouse IgG2a monoclonal antibody (clone PPV-04) reacts with undefined epitope on a plant pathogen.
Clone number:
PPV-04
Antibody Isotype:
IgG2a
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
TCR alpha/beta-negative CD3-positive rat T cell hybridoma III.89.1.4 line
Applications:
FC,IP,IHC
Additional Info:
The mouse monoclonal antibody V65 recognizes an extracellular epitope of TCR gamma/delta, the subtype of T cell receptor expressed mainly in epithelial tissues and at the sites of infection.
The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Rat T blasts and erythrocytes
Applications:
FC,IP,WB,IHC,ICC
Additional Info:
The mouse monoclonal R73 recognizes an extracellular epitope TCR alpha/beta, the dominant subtype of T cell receptor expressed in peripheral blood.
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ?g/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody PPV-04, generated against a plant pathogen, does not cross-react with other species, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG2a molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Applications:
FC,IP,IHC,ICC,WB,ELISA,FA
Additional Info:
This mouse IgG2a monoclonal antibody (clone PPV-04) reacts with undefined epitope on a plant pathogen.
Clone number:
PPV-04
Antibody Isotype:
IgG2a
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ?g/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-173, generated against an undefined antigen, does not react specifically with mouse, rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG2a molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Product Type:
Antibodies Isotype Control
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
The transplantable plasmacytoma MOPC-173 was induced by intraperitoneal injection of mineral oils into BALB/c mice.
Applications:
FC,IP,WB,ICC,IHC,ELISA,FA
Additional Info:
This mouse IgG2a monoclonal antibody (clone MOPC-173) reacts with an unknown epitope. It does not react with a variety of resting, activated, live, and fixed mouse, rat and human tissues.
Clone number:
MOPC-173
Antibody Isotype:
IgG2a k
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
The CD8b (CD8 beta) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages, mast cells, and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
CD8 positive Wistar rat splenic T cell hybridomas
Applications:
FC,IP,WB,IHC
Additional Info:
The mouse monoclonal antibody 341 (also known as 34.1) recognizes rat CD8b, the 32-34 kDa beta chain of the CD8 coreceptor (extracellular epitope), expressed on T cell subsets and some other cell types, such as macrophages.
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ?g/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MPC-11 was generated against an epitope irrelevant for human, mouse, and rat material, and can thus be used for evaluation of the background staining that is caused by general nonspecific interactions between an mouse IgG2b molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Product Type:
Antibodies Isotype Control
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
KLH-coupled trinitrophenol
Applications:
ELISA,FC,IP,WB,IHC,ICC
Additional Info:
This mouse IgG2b (kappa) monoclonal antibody (clone MPC-11) reacts with an epitope irrelevant for a variety of resting, activated, live, and fixed human, mouse, and rat tissues.
Clone number:
MPC-11
Antibody Isotype:
IgG2b k
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
The CD8a (CD8 alpha) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
High Mw glycoproteins from rat thymocytes
Applications:
FC,IP,WB,IHC
Additional Info:
The mouse monoclonal antibody OX-8 recognizes the hinge-like membrane-proximal extracellular domain of rat CD8a (32-34 kDa; alpha chain of the CD8 antigen).
CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
MLR generated rat Th cells
Applications:
FC,IHC,ICC
Additional Info:
The mouse monoclonal antibody OX-35 reacts with an extracellular epitope of rat CD4 transmembrane glycoprotein (55 kDa).
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ?g/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody PLRV219, generated against a plant pathogen, does not cross-react with other species, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG2b molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished. _x000D_
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Applications:
FC,IP,IHC,ICC,WB,ELISA
Additional Info:
This mouse IgG2b monoclonal antibody (clone PLRV219) reacts with undefined epitope on a plant pathogen.
Clone number:
PLRV219
Antibody Isotype:
IgG2b
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Unlike human, the murine protein is expressed only as a transmembrane isoform. Also CD32 (FcgammaRII) is a low affinity receptor for IgG, but its affinity is lower than that of CD16. These receptors are expressed on monocytes/macrophages, NK cells, granulocytes, mast cells, dendritic cells, and B cells. Their role is to mediate adaptive immune responses through binding the antibody-antigen immunocomplexes, but their effect on the particular cell differs according to the cell type.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Murine pre-B cells
Applications:
FC
Additional Info:
The rat monoclonal antibody 93 recognizes a common extracellular epitope of murine CD16 (FcgammaRIII) and CD32 (FcgammaRII), the low affinity receptors for IgG.
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ?g/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody PPV-07, generated against a plant pathogen, does not cross-react with other species, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG3 molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Applications:
FC,IP,IHC,ICC,WB,ELISA
Additional Info:
This mouse IgG3 monoclonal antibody (clone PPV-07) reacts with undefined epitope on a plant pathogen.
Clone number:
PPV-07
Antibody Isotype:
IgG3
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ?g/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody PFR-03, generated against a plant pathogen, does not cross-react with other species, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgM molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Applications:
FC,IP,IHC,ICC,WB,ELISA
Additional Info:
This mouse IgM monoclonal antibody (clone PFR-03) reacts with undefined epitope on a plant pathogen.
Clone number:
PFR-03
Antibody Isotype:
IgM
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Mouse A20J B lymphoma cells transfected with rat CD28
Applications:
FC,IP
Additional Info:
The mouse monoclonal antibody JJ319 reacts with an extracellular epitope of CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.
CD161, also known as Nkrp1 (natural killer receptor protein 1) or Klrb1 (killer cell lectin-like receptor subfamily b member 1), is a disulphide-linked homodimeric receptor, which is involved in regulation of NK cell and NKT cell function. It is expressed on rat NK cells, subset of T cells, dendritic cells, and activated monocytes. Although human CD161 is expressed as one isoform, the rat CD161 has three isoforms, referred to as CD161a, b, and c. These proteins contain C-terminal C-type lectin extracellular domain, a transmembrane domain, and N-terminal intracellular domain, which contains ITIM motif, such as CD161b, and displays inhibitory function, or does not contain ITIM motif, thus also not the inhibitory function, such as CD161a.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Splenic cells purified from the LEW rat
Applications:
FC,IP,WB,IHC,RIA
Additional Info:
The mouse monoclonal antibody 10/78 recognizes CD161, an approximately 30 kDa type II transmembrane C-type lectin receptor, expressed on the plasma membrane of NK cells, dendritic cells, activated monocytes and a subset of T cells as a disulphide-linked homodimer. A common extracellular epitope on rat CD161a and b isoforms is detected.
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkynje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Mouse BM10-37 cytotoxic T lymphocytes
Applications:
FC
Additional Info:
The Armenian hamster monoclonal antibody 145-2C11 reacts with an extracellular epitope of murine CD3 (epsilon subunit). This antibody is commonly used as a phenotypic marker for murine T cells.
CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Mouse CTL clone V4 cells
Applications:
FC
Additional Info:
The rat monoclonal antibody GK1.5 reacts with an extracellular epitope of mouse CD4 transmembrane glycoprotein (55 kDa).
CD41 (platelet glycoprotein IIb) is composed of two subunits (120 kDa a, alpha and 23 kDa b, beta) that interact with CD61 in the presence of calcium to form a functional adhesive protein receptor. Upon blood vessel damage, this receptor binds to a variety of proteins including von Willebrand factor, fibrinogen, fibronectin and vitronectin. CD41 is mainly expressed on megakaryocyte-platelet lineage, but generally belongs to the antigens that are expressed during early stages of hematopoietic differentiation.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Murine platelets
Applications:
FC,IP,IHC,ELISA
Additional Info:
The rat monoclonal antibody MWReg30 recognizes an extracellular epitope of CD41 (GPIIb), a transmembrane glycoprotein (integrin family) composed of two chains GPIIb alpha (heavy chain; 120 kDa) and GPIIb beta (light chain; 23 kDa). CD41 is mainly expressed on platelets and megakaryocytes.
This rat IgG2a kappa monoclonal antibody (clone RTG2A1-1, also known as G2A-1-1 or KLH/G2A-1-1) detects specifically the hemocyanin of keyhole limpet and can be used as a negative control antibody in other species.
Clone number:
RTG2A1-1
Antibody Isotype:
IgG2a
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
CD41 (platelet glycoprotein IIb) is composed of two subunits (120 kDa a, alpha and 23 kDa b, beta) that interact with CD61 in the presence of calcium to form a functional adhesive protein receptor. Upon blood vessel damage, this receptor binds to a variety of proteins including von Willebrand factor, fibrinogen, fibronectin and vitronectin. CD41 is mainly expressed on megakaryocyte-platelet lineage, but generally belongs to the antigens that are expressed during early stages of hematopoietic differentiation.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Murine platelets
Applications:
FC,IP,IHC,ELISA,FA
Additional Info:
The rat monoclonal antibody MWReg30 recognizes an extracellular epitope of CD41 (GPIIb), a transmembrane glycoprotein (integrin family) composed of two chains GPIIb alpha (heavy chain; 120 kDa) and GPIIb beta (light chain; 23 kDa). CD41 is mainly expressed on platelets and megakaryocytes.
This rat IgG2a kappa monoclonal antibody (clone RTG2A1-1, also known as G2A-1-1 or KLH/G2A-1-1) detects specifically the hemocyanin of keyhole limpet and can be used as a negative control antibody in other species.
Clone number:
RTG2A1-1
Antibody Isotype:
IgG2a
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
This rat IgG2b kappa monoclonal antibody (clone RTG2B1-2, also known as G2B-1-2 or KLH/G2B-1-2) detects specifically the hemocyanin of keyhole limpet and can be used as a negative control antibody in other species.
Clone number:
RTG2B1-2
Antibody Isotype:
IgG2b
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
This rat IgG2b kappa monoclonal antibody (clone RTG2B1-2, also known as G2B-1-2 or KLH/G2B-1-2) detects specifically the hemocyanin of keyhole limpet and can be used as a negative control antibody in other species.
Clone number:
RTG2B1-2
Antibody Isotype:
IgG2b
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells._x000D_
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Immunogen:
Dexamethasone-induced cells of the SJL mouse spontaneous myeloid leukemia M1_x000D_
Applications:
FC,IP,WB,IHC,ICC
Additional Info:
The rat monoclonal antibody IM7 reacts with CD44 antigen (Phagocyte glycoprotein 1), an 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes. The antibody reacts with all isoforms of mouse CD44._x000D_
CD90 (Thy-1) is an 18-35 kDa GPI-anchored plasma membrane glycoprotein expressed in many cell types, such as in hematopoietic cells and neurons, connective tissues, various fibroblast and stromal cell lines, tumor endothelial cell lines and other. In the mouse, CD90 is expressed mainly on thymocytes and peripheral T lymphocytes. It is involved in T cell activation, cellular adhesion, proliferation and migration, neurite outgrowth, wound healing, apoptosis, and fibrosis. CD90 participates in multiple signaling cascades and its effects are tissue- and cell type-specific. It often functions as an important regulator of cell-cell and cell-matrix interactions.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
thymocytes from C3H/Di mice (Thy-1.2 positive)
Applications:
FC
Additional Info:
The mouse monoclonal antibody 1aG4/C5 recognizes alloantigen Thy-1.2 of mouse Thy-1 (CD90); it does not cross-react with Thy-1.1 alloantigen. Thy-1 is an 18-35 kDa cell surface GPI anchored glycoprotein (immunoglobulin superfamily); in mouse it is abundant on thymocytes and peripheral T lymphocytes.
Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Purified human IgE.
Applications:
ELISA,FC
Additional Info:
The mouse monoclonal antibody 4G7 reacts with human IgE; it recognizes an epitope different from the ones recognized by BE5 and 4H10 antibodies to IgE. The epitope is located within the amino acids 103-115 (WSDYNFDYSSSEE).
The CD8a (CD8 alpha) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Murine spleen cells
Applications:
FC
Additional Info:
The rat monoclonal antibody 53-6.7 recognizes an extracellular epitope of murine CD8a (32-34 kDa; alpha chain of the CD8 antigen).
CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
LPS-activated CBA/Cs mouse splenic B cells
Applications:
FC,IP,IHC,ICC,FA
Additional Info:
The rat monoclonal antibody GL-1 reacts with an extracellular epitope of CD86 (B7-2), a 70-80 kDa type I transmembrane glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.
CD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associated tetraspanin-enriched signaling microdomains within the plasma membrane, forming complexes with each other as well as with integrins, membrane-anchored growth factors and other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 is also considered as metastasis suppressor in solid tumors.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Permeabilized murine bone marrow-derived mast cells (BMMC).
Applications:
FC
Additional Info:
The rat monoclonal antibody EM-04 recognizes an extracellular epitope of CD9 antigen, a 24 kDa transmembrane protein expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes.
Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Purified human IgE.
Applications:
FC,ELISA
Additional Info:
The antibody BE5 reacts with human IgE; it recognizes an epitope different from the ones recognized by 4G7 and 4H10 antibodies to IgE.
CD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associated tetraspanin-enriched signaling microdomains within the plasma membrane, forming complexes with each other as well as with integrins, membrane-anchored growth factors and other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 is also considered as metastasis suppressor in solid tumors.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Permeabilized murine bone marrow-derived mast cells (BMMC).
Applications:
FC,IP,WB,ICC
Additional Info:
The rat monoclonal antibody EM-04 recognizes an extracellular epitope of CD9 antigen, a 24 kDa transmembrane protein expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes.
LEA (Late embryogenesis abundant) proteins are very hydrophilic proteins, described over 25 years ago as accumulating during late stages of plant seed development. Found in vegetative plant tissues following exposure to environmental stress.Synonymes: Putative late embryogenesis abundant protein LEA.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana (recombinant LEA6)
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from Arabidopsis thaliana LEA6 protein sequences, UniProt: O64820, TAIR: At2g23110, UniProt: Q8S8R1 TAIR: AT2G23120 and UniProt: O23658, TAIR: AT2G33690
Goat anti-Rabbit IgG (H&L), F(ab)'2 Fragment, min. cross-reactivity to non-immunoglobulin rabbit serum proteins and human serum proteins is a secondary antibody, which binds to rabbit IgG (H&L), F(ab)'2 Fragment in immunological assays.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Antibody purity is > 90% based on SDS-PAGE .Affinity purified using solid phase Rabbit IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on rabbit IgG • light chains on all rabbit immunoglobulins .Based on IEP, no reactivity is observed to: • non-immunoglobulin rabbit serum proteins • serum proteins from human.
Immunoglobulin A (IgA) is the major immunoglobulin class in body secretions that protects against infections of the mucous membranes lining the mouth, airways, and digestive tract. Its serum concentration is approximately 0.2 g/l and biological survival (T1/2 in plasma) 6 days. Plasma IgA is predominantly monomeric (160 kDa) and of the IgA1 subclass, and is mostly produced in the bone marrow. Over half of IgA produced by the intestinal lamina propria plasmocytes is in oligomeric form and 35% of it is IgA2.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Purified human IgA.
Applications:
IHC,ELISA
Additional Info:
The mouse monoclonal antibody AD3 reacts with alpha-chain of human IgA1 and IgA2.
Immunoglobulin D (IgD) is expressed on the surface of naive mature B cells, thus later than IgM, and is coexpressed with it then. Triggered by antigen binding, it signals through the CD79 complex to activate the B cells. Expression of IgD is lost after the isotype switch. Soluble IgD is present in very small amounts in the serum. IgD can bind to basophils and mast cells to activate them in an IgE-independent way to participate in respiratory immune defense.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Human IgD
Applications:
FC
Additional Info:
The mouse monoclonal antibody IA6-2 recognizes human immunoglobulin D.
Ractopamine is a phenolethanolamine ß-adrenoceptor agonist that is used for the improvement of weight gain, carcass leanness and feed efficiency in pigs. It has been known for years that ractopamine enhances growth in meat animals by repartitioning nutrients. This means that the nutrients that the animal consumes in its feed are shifted away from being stored as fat and are instead used in lean tissue production. This shift occurs as the animal reaches the top of its growth curve and lean tissue production drops off and fat deposition increases. Fat levels also appear to decrease, due to an increase in lypolysis (fat breakdown). Using the ractopamine monoclonal antibody, it is possible to detect ractopamine and other agonists in urine, muscle and liver both rapidly and with accuracy.
Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75% of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Purified human IgG.
Applications:
FC,WB,ELISA
Additional Info:
The mouse monoclonal antibody 4A11 reacts with Fab fragment of human IgG.
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Country Of Origin:
Normal Pig Serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Rabbit
Immunogen:
Purified Hamster IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on hamster IgG · light chains on all hamster immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin hamster serum immunoglobulins · human serum proteins
Country Of Origin:
Rabbit serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution:
Rehydrate with 11.0 ml of deionized water.(Product has been overfilled to ensure complete recovery.) Swirl gently and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates.
Storage:
Store lyophilized material at 2-8 °C. For long term storage after reconstitution, prepare small aliquots and store at -20 °C . For storage at 2-8 °C, add a preservative to prevent growth of bacteria.
Specificity:
non-immune
Country Of Origin:
Normal Rabbit Serum was obtained from healthy animals of US origin.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Purified human IgE.
Applications:
FC,ELISA
Additional Info:
The mouse monoclonal antibody 4H10 reacts with human IgE; it recognizes an epitope different from the ones recognized by BE5 and 4G7 antibodies to IgE. The epitope is located within the amino acids 267-279 (TWLEDGQVMDVDL).
The reactivity of the antiserum is restricted to the Fc part of the IgM molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal human serum a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgM. No precipitation reaction is obtained with purified IgG, IgA, and IgG/Fab fragments. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in human serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of human IgM from serum or plasma.The antiSerum does not cross-react with any other component of the Human Ig system. Interspecies cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. In immunoelectrophoresis crossreactivity of this antiSerum has not been observed with Serum of Rhesus Monkey, Cynomolgus and Baboon.
Goat anti-Rabbit IgG (H&L), F(ab)'2 Fragment, DyLight 488 conjugated is a secondary antibody conjugated to DyLight 488, which binds to rabbit IgG in immunological assays. DyLight 488 has Amax = 493 nm, Emax = 518 nm. DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
For reconstitution add 0,55 ml of sterile water,Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Antibody purity is > 90% based on SDS-PAGE Small amounts of intact IgG may be present.Affinity purified using solid phase Rabbit IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on rabbit IgG • light chains on all rabbit immunoglobulins .Based on IEP, no reactivity is observed to: • non-immunoglobulin rabbit serum proteins .
Goat anti-Donkey IgG (H&L), DyLight 633 conjugated is a secondary antibody conjugated to DyLight 633, which binds to Donkey IgG (H&L) in immunological assays. DyLight 633 has Amax = 638 nm, Emax = 658 nm.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
For reconstitution add 1,1 ml of sterile water,Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Donkey IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on donkey IgG • light chains on all donkey immunoglobulins .Based on IEP, no reactivity is observed to: ��� non-immunoglobulin donkey serum immunoglobulins .
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on Pig IgG · light chains on all Pig immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin Pig serum immunoglobulins
Country Of Origin:
Normal Goat Serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Goat anti-rabbit IgG (H&L) is a secondary antibody conjugated to HRP which binds to all rabbit IgGs in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water, add 1.1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Based on IEP, this antibody reacts with: rabbit IgG heavy chains and light chains on all rabbit immunoglobulins
Immunogen:
Purified Rabbit IgG, whole molecule,
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Immunogen affinity purified using solid phase rabbit IgG.
Reconstitution:
For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Spin centrifuge shortly to remove any particles. Prepare fresh working dilutions daily
Not reactive in:
Non-immunoglobulin rabbit serum proteins
Selected references:
Lim et al (2022). Arabidopsis guard cell chloroplasts import cytosolic ATP for starch turnover and stomatal opening. Nat Commun. 2022 Feb 3;13(1):652. doi: 10.1038/s41467-022-28263-2. PMID: 35115512; PMCID: PMC8814037.Miklankova et al. (2022) HYPK promotes the activity of the Nalfa-acetyltransferase A complex to determine proteostasis of nonAc-X2/N-degron-containing proteins. Sci Adv. 2022 Jun 17;8(24):eabn6153. doi: 10.1126/sciadv.abn6153. Epub 2022 Jun 15. PMID: 35704578; PMCID: PMC9200280.Hofmann, Wienkoop & Luthje (2022) Hypoxia-Induced Aquaporins and Regulation of Redox Homeostasis by a Trans-Plasma Membrane Electron Transport System in Maize Roots. Antioxidants (Basel). 2022 Apr 25;11(5):836. doi: 10.3390/antiox11050836. PMID: 35624700; PMCID: PMC9137787.Bychkov et al. (2022) The role of PAP4/FSD3 and PAP9/FSD2 in heat stress responses of chloroplast genes. Plant Sci. 2022 Sep;322:111359. doi: 10.1016/j.plantsci.2022.111359. Epub 2022 Jun 20. PMID: 35738478.Vitale et al. (2021) Light Spectral Composition Influences Structural and Eco-Physiological Traits of Solanum lycopersicum L. cv. 'Microtom' in Response to High-LET Ionizing Radiation. Plants (Basel). 2021 Aug 23;10(8):1752. doi: 10.3390/plants10081752. PMID: 34451797; PMCID: PMC8399554.
Special application note:
Concentration: 1.0 mg/ml.Antibody is provided in: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1% BSA (w/v), Protease IgG free, 0.1 % (v/v) ProClin 150.Affinity purified antibody is >95% pure, according to SDS-PAGE.This antibody can used on a very wide range of samples from various species including many model plants, algae, diatoms and bacteria.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Human IgM, µ chain
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Specificity:
Based on IEP, this antibody reacts with: · heavy (µ) chains on human IgM
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin human serum proteins · light chains on all human immunoglobulins · human IgG · bovine, goat, mouse, or rabbit serum proteins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
The HER2/neu (c-erbB-2) proto-oncogene is a transmembrane receptor tyrosine kinase that is clinically indicated in a number of carcinomas. Overexpression of the c-erbB-2 protein has been associated with ductal breast cancer, as well as pulmonary and gastric adenocarcinomas. A correlation between HER2 and p53 has also been documented, as overexpression of both proteins has been associated with early invasion and metastasis in bladder cancer.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC002
Antibody Isotype:
IgG2a
GMDN Code:
57047
UKCA Status:
RUO
CE-IVD Status:
RUO
Positive Control:
Breast Carcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Caldesmon is a marker for smooth muscle differentiation. Found in smooth muscle and other tissues, caldesmon interacts with Ca<sup>2+</sup>-calmodulin, actin, tropomyosin, myosin, and phospholipids. It inhibits the ATPase activity of myosin in smooth muscle, and mediates Ca<sup>2+</sup>-dependent inhibition of smooth muscle and non-muscle contraction. Caldesmon expression is found in Gastrointestinal Stromal Tumours (GIST), and can be used to differentiate epithelioid mesothelioma from serous papillary carcinoma of the ovary. It is also a specific marker for smooth muscle cells (SMC) and associated neoplasms; therefore, Anti-Caldesmon can be used in the study of the SMC differentiation process as well as the differentiation of other tumours with SMC-like differentiation, including leiomyosarcoma and myofibroblastic tumours.
Annexin A1 (ANXA1) is a membrane protein that plays a role in innate and adaptive immunity by controlling the biosynthesis of inflammation, prostaglandins, and leukotriene mediators. This target is overexpressed in 97% of all samples from patients with hairy cell leukemia, and is absent in other B-cell lymphomas. High ANXA1 expression is frequently associated with advanced stage esophageal and esophagogastric junction adenocarcinoma, and is also linked to advanced and metastatic disease states.
CA 19-9 is a secreted protein that is implicated in various cancers. It is overexpressed in salivary gland mucoepidermoid carcinomas and gastric, pancreatic, and colonic (gastrointestinal) adenocarcinomas, but is not expressed in breast, kidney, and prostate carcinomas. CA 19-9 staining is also implicated in Mirizzi’s Syndrome or other bile duct and liver diseases.
BAP1 (BRCA1 associated protein-1 or ubiquitin carboxy-terminal hydrolase) is a deubiquitinating enzyme that in humans is encoded by the BAP1 gene. BAP1 encodes an 80.4 kDa nuclear-localizing protein with a ubiquitin carboxy-terminal hydrolase (UCH) domain that gives BAP1 its deubiquitinase activity. In cancer, BAP1 can function both as a tumor suppressor and as a metastasis suppressor.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC039
Antibody Isotype:
IgG2b
GMDN Code:
?
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Breast
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
The HER2/neu (c-erbB-2) proto-oncogene is a transmembrane receptor tyrosine kinase that is clinically indicated in a number of carcinomas. Overexpression of the c-erbB-2 protein has been associated with ductal breast cancer, as well as pulmonary and gastric adenocarcinomas. A correlation between HER2 and p53 has also been documented, as overexpression of both proteins has been associated with early invasion and metastasis in bladder cancer.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC042
Antibody Isotype:
IgG2a
GMDN Code:
57047
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Breast Carcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Cluster of Differentiation 61 (CD61), also known as Glycoprotein IIIa or GPIIIa, is an antigen expressed on megakaryocytes, platelets, myeloid cells, monocytes, endothelial cells, smooth muscle cells, and macrophages. It is involved in platelet aggregation and acts as a receptor for fibrinogen, fibronectin, von Willebrand factor, and vitronectin. Anti-CD61 is used for identifying megakaryocytopoiesis, as seen in megakaryoblastic leukemias, myelodysplastic disorders, and acute myeloid leukemias. CD61 is also indicated as a marker for platelet adhesion in advanced atherosclerosis and has been reported in the identification of fat embolism in pulmonary tissue.
Arginase-1, encoded by the ARG1 gene, is a cytosolic metalloenzyme expressed predominantly in hepatocytes. Arginase-1 plays a key role in the urea cycle by catalyzing the hydrolysis of arginine to ornithine and urea. Argininemia is an inherited autosomal recessive disorder characterized by a buildup of arginine and ammonia in the blood. Anti-Arginase-1 is highly specific for hepatocytes, and is therefore a sensitive and specific marker of benign and malignant hepatic tumours.
BG8 Lewis y, also known as Lewis<sup>y</sup> Blood Antigen or simply BG8, is a blood group antigen that has been identified in many studies as a potential marker for differentiation between pulmonary adenocarcinoma (PACA) and epithelioid mesothelioma (EM). It has been reported that sensitivity of non-mesothelial antigens for adenocarcinoma is organ-dependent. When attempting to differentiate epithelioid mesothelioma from adenocarcinoma, BG8 Lewis<sup>y</sup> performed at a sensitivity of 98% in the breast cancer group, and 100% in the lung cancer group.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC517
Antibody Isotype:
IgG1, kappa
GMDN Code:
63793
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Lung Adenocarcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
HMB-45 is specific for an antigen present in immature melanosomes, cutaneous melanocytes, and prenatal and infantile retinal pigment epithelium cells. It is therefore effective for identifying malignant melanoma, and differentiating metastatic amelanotic melanoma from a number of conditions where the discrimination is often extremely difficult, including large cell lymphomas, sarcomas, spindle cell carcinomas, and various types of mesenchymal neoplasms. This antibody can also differentiate between junctional nevus and intradermal nevus cells, and between fetal or neonatal melanocytes and normal adult melanocytes.
The Human Equilibrative Nucleoside Transporter 1 (hENT1) mediates the cellular uptake of physiologic nucleosides, including adenosine, as well as many anti-cancer drugs including gemcitabine, cytarabine, and decitabine. Deficiency of hENT1 can lead to resistance of such drugs, and the abundance of hENT1 protein in the plasma membrane is a major indicator of the efficiency and clinical outcome of these anti-cancer nucleosides.
Bile Salt Export Pump (BSEP) is a member of the ATP-binding cassette (ABC) transporters, and mediates the transport of bile acid, taurocholate, and other cholate conjugates across the hepatocyte canalicular membrane into the canaliculus. BSEP is associated with progressive familial intrahepatic cholestasis type 2 (PFIC2) and benign recurrent intrahepatic cholestasis type 2 (BRIC2). PFIC2 caused by mutations in the BSEP gene increases the risk of hepatocellular carcinoma in early life.
Cluster of Differentiation 73 (CD73), also known as Ecto-5'-Nucleotidase (ecto-5'-NT), is a cell surface enzyme found in most tissues. CD73 catalyzes the breakdown of AMP to adenosine, thereby modulating inflammatory and T-cell responses. Reports have implicated CD73 expression in tumour progression and carcinogenesis, as CD73 is a key regulatory molecule in the proliferation, migration, and invasion of cancer cells <em>in vitro</em>, as well as tumour angiogenesis and tumour immune escape <em>in vivo</em>. Due to this key involvement in cancer, CD73 has become an appealing target for cancer immunotherapy. CD73 expression has also been linked to favourable prognosis in breast carcinoma.
The C4d [IHC519] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissue within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
B7H4 is a glycosylated transmembrane protein of the B7 family. It binds to activated T cells to moderate the T cell responses via cell cycle arrest in the T cell. Reverse signaling can induce either cell cycle arrest or apoptosis in the B7H4 expressing cell. B7H4 is up-regulated in several carcinomas in correlation with tumor progression and metastasis. A soluble form of B7H4 is elevated in the serum of ovarian cancer, renal cell carcinoma, and rheumatoid arthritis patients, also in correlation with advanced disease status.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC039
Antibody Isotype:
IgG2b
GMDN Code:
?
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Breast
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Total IgG. Protein A purified in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2. Contains 0.05 % sodium azide.
Special application note:
Human IgG is provided in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. 0.005 % sodium azide is added as preservative.Concentration is 4.5 mg/ml.
B-Cell Lymphoma 2 (BCL2) is involved in regulation of cell apoptosis by controlling mitochondrial permeability and release of cytochrome c. It also has critical roles in normal cell physiology related to neuronal activity, autophagy, calcium handling, mitochondrial dynamics, and energetics. BCL2 overexpression has been shown to promote cell survival by suppressing apoptosis, and is found to be correlated with poor disease prognosis in breast, prostate, ovarian, endometrial, and colon cancers. In follicular lymphoma, Anti-BCL2 reacts negatively with germinal centers and positively with neoplastic follicles. In lymphoid lesions, BCL2 staining is useful for distinguishing reactive and neoplastic follicular proliferations, and for identifying minimal residual disease in the bone marrow of follicular lymphoma patients. BCL2 is now a useful target of human cancer therapy.
The Cadherin-17 [IHC520] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissue within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist
ATRX is involved in the remodeling of the nucelosome structure, and facilitate the transcription and replication. ATRX loss occurs in grades II/III astrocytomas and glioblastomas and comes together with IDH1 mutations. Detection of ATRX with IHC acts a sensitive method to identify the mutations.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC703
Antibody Isotype:
IgG2b
GMDN Code:
65252
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Breast
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Androgen Receptor (AR) is a transcriptional regulator with a broad array of functions. This marker is clinically significant in the understanding of tumour progression and tumour aggressiveness. The detection of AR by immunohistochemical staining is important for diagnosis of all types of prostate carcinoma, including both therapy-responsive and therapy-unresponsive disease states. Co-testing with AR and CK20 is used for differential diagnosis of desmoplastic trichoepithelioma (DTE) [CK20+/AR-], morpheaform basal cell carcinoma (BCC) [CK20-/AR+], and microcystic adnexal carcinoma (MAC) [CK20-/AR-].
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC511
Antibody Isotype:
IgG
GMDN Code:
56796
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Prostate Carcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Indoleamine 2,3-dioxygenase 1 (IDO1) is a cytoplasmic enzyme encoded by the INDO gene on human chromosome 8p22. IDO1 modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in immune evasion and tumor outgrowth. Blocking the IDO1 pathway may be a potential target for immuno and cancer therapy. IDO1 expressions have been found in endometrial, cervical carcinomas, renal cell carcinomas, non-small cell lung carcinomas, and colorectal carcinomas.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC101
GMDN Code:
66572
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Tonsil
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
The IgG4 [IHC114] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissues within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Applications:
IHC
Clone number:
IHC114
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Tonsil
Buffer:
Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
Cluster of Differentiation 63 (CD63) is a lysosomal membrane glycoprotein identified as a platelet activation molecule. CD63 localizes to the membrane and cytoplasm of many cell types including lymphoid, myeloid, and endothelial cells. CD63 is a useful marker for malignant melanoma, and for distinguishing between renal oncocytoma and eosinophilic renal cell carcinoma.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC540
Antibody Isotype:
IgG1, kappa
GMDN Code:
62544
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Melanoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Calponin is an actin, tropomyosin and calmodulin-binding protein that involves in the regulation of smooth muscle contraction. This antibody is mainly used for the diagnosis and research of myoepithelial cells in leiomyoma and breast lesions.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC075
Antibody Isotype:
IgG
GMDN Code:
56877
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Thyroid, Thyroid Medullary Carcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Actin is part of the cytoskeletal system of all cell types. Smooth muscle actin is found in myofibroblasts and myoepithelium, but not in cardiac or skeletal muscles. Labeling of smooth muscle actin in concert with muscle specific actin staining can allow for differentiation between rhabdomyosarcoma and leiomyosarcoma, as muscle-specific actin is found in rhabdomyoblasts, while smooth muscle actin is found in leiomyosarcomas.
Actin is part of the cytoskeletal system of every cell type. It can be classified based on isoelectric points as alpha, beta, and gamma. Muscle Specific Actin includes those of the alpha and gamma isotypes. Skeletal, smooth, and cardiac muscle cells will all stain positively with Anti-Muscle Specific Actin, but mesenchymal cells, not including myoepithelium, will stain negatively. Normal and neoplastic non-muscle cells, including vascular endothelial and connective tissues, carcinomas, melanomas, and lymphomas, will also be negative for muscle specific actin. The use of Anti-Muscle Specific Actin in concert with Anti-Smooth Muscle Actin can allow for differentiation between rhabdomyosarcoma and leiomyosarcoma, as muscle specific actin is found in rhabdomyoblasts, while smooth muscle actin is found in leiomyosarcomas.
CDK4 controls cell growth during the cell cycle G1 phase. It is reported that D-type cyclin upregulates Cdk4 activity, whereas binding to the Cdk inhibitor p16 downregulates Cdk4 activity. Cyclin D-CDK4 complexes integrates various mitogenenic and antimitogenic signals and serves as sensitive and specific for atypical lipomatous tumor / well differentiated liposarcoma or dedifferentiated liposarcoma
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Applications:
IHC
Clone number:
IHC077
UKCA Status:
UKCA
CE-IVD Status:
RUO
Buffer:
Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
Cluster of Differentiation 7 (CD7) is an antigen expressed in immature and mature T-lymphocytes, thymocytes, peripheral blood T-cells, natural killer cells, myeloid precursors, fetal liver and bone marrow, a small subpopulation of normal B-cells, and malignant B-cells. The antigen belongs to the immunoglobulin gene superfamily, and plays an important role in T-cell interactions and T-cell/B-cell interactions during early lymphoid development. CD7 is indicated as a marker for acute myelogenous leukemia and chronic myelogenous leukemia, and for neoplastic proliferations such as T-cell acute lymphoblastic leukemia/lymphoma. Anti-CD7, when used in adjunct with Anti-CD4, is useful for differentiating mycosis fungoides or Sézary syndrome (both cutaneous T-cell lymphomas) from benign dermatoses.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC541
GMDN Code:
56934
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Tonsil, Peripheral T-Cell Lymphoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Calcitonin is a polypeptide hormone formed by the proteolytic cleavage of a larger prepropeptide. It is produced primarily by the parafollicular C-cells of the thyroid, and is involved in the regulation of calcium and phosphorus metabolism. It decreases the level of calcium and phosphate ions in blood by promoting the incorporation of these ions into bones, as well as inhibiting renal tubular cell reabsorption. Calcitonin expression is found in C-cell hyperplasia and medullary thyroid carcinomas. It is a useful marker in the identification of C-cell proliferative abnormalities, and for distinguishing medullary carcinoma from papillary and follicular thyroid cancer.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC521
Antibody Isotype:
IgG
GMDN Code:
56870
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Thyroid, Thyroid Medullary Carcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
<em>Helicobacter pylori</em> are spiral-shaped, gram-negative bacteria that inhabit the mucosal lining of the gastric epithelium. Infection with <em>H. pylori</em> is strongly associated with many gastroduodenal diseases, including intestinal-type carcinomas, peptic and gastric ulcers, and chronic gastritis. There is evidence linking these bacteria to gastric and mucosa-associated lymphoid tissue lymphomas, and <em>H. pylori</em> has also been indicated as a risk factor for colorectal polyps in children.
Adrenocorticotropic Hormone (ACTH or Corticotropin) is a peptidic hormone synthesized in the anterior pituitary gland. The primary application of Anti-ACTH is in the identification of pituitary tumours and the study of pituitary disease. The Anti-ACTH antibody reacts with ACTH-producing cells (corticotrophs). It may also cause paraneoplastic syndromes by secreting ACTH from other tumours, such as some small cell carcinomas of the lung.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC503
Antibody Isotype:
IgG1
GMDN Code:
56764
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Skeletal Muscle
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Cluster of Differentiation 8 (CD8) is a transmembrane glycoprotein that serves as a co-receptor for the T-cell receptor. It is expressed in cytotoxic T-cells, natural killer cells, cortical thymocytes, some null cells, and bone marrow cells. Anti-CD8, in a panel of other antibodies, may be used to differentiate between reactive and neoplastic T-lymphocytes.
Cluster of Differentiation 99 (CD99) is a glycosylated transmembrane protein expressed by lymphocytes, cortical thymocytes, granulosa cells of the ovary, pancreatic islet cells, Sertoli cells, and endothelial cells. CD99 produces diffuse membrane staining patterns on nearly all Ewing's sarcoma and primitive peripheral neuroectodermal tumours. CD99 may be found in synovial sarcoma, neuroendocrine carcinoma, acute myeloid leukemia, mesenchymal chondrosarcoma, lymphoblastic lymphoma, small round blue cell tumours, solitary fibrous tumours, vascular tumours, and myeloid sarcoma. It produces heterogeneous staining patterns which must be accompanied by other antibody staining for a final diagnosis.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC126
Antibody Isotype:
IgG1
GMDN Code:
57028
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Ewings Sarcoma, Pancreas
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
The BCA-225 [IHC225] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissues within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC225
GMDN Code:
?
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Breast Carcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Aldo-Keto Reductase Family 1 Member B10 (AKR1B10) is an enzyme of the aldo-keto reductase superfamily, and catalyzes the reduction of aliphatic and aromatic aldehydes. AKR1B10 is commonly expressed in adrenal glands, the small intestine, and colon tissues. AKR1B10 staining is useful in the recognition of liver carcinogenesis.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC508
Antibody Isotype:
IgG2a
GMDN Code:
?
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Hepatocellular Carcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Cluster of Differentiation 57 (CD57), also known as NK-1, is an antigen detectable in natural killer cells, some T-lymphocytes and normal peripheral blood mononuclear cells, myeloid cells, and a variety of polypeptides, lipids, and chondroitin sulfate proteoglycans. CD57 is indicated as a marker for tumours of neuroendocrine origin, including pheochromocytomas, paragangliomas, medulloblastomas, and carcinoid tumour, as well as various neural tumours including neuromas, neurofibromas, schwannomas, and granular cell tumours. CD57 is also detectable in ganglioneuroma and prostate carcinoma. Anti-CD57 is used to distinguish nodular lymphocyte-predominant Hodgkin's lymphoma from T-cell/histiocyte-rich large B-cell lymphoma, nodular sclerosis Hodgkin's disease, and follicular lymphoma.
The HER2/neu (c-erbB-2) proto-oncogene is a transmembrane receptor tyrosine kinase that is clinically indicated in a number of carcinomas. Overexpression of the c-erbB-2 protein has been associated with ductal breast cancer, as well as pulmonary and gastric adenocarcinomas. A correlation between HER2 and p53 has also been documented, as overexpression of both proteins has been associated with early invasion and metastasis in bladder cancer.
Product Type:
Primary Antibody
Antibody Type:
Polyclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC012
Antibody Isotype:
IgG
GMDN Code:
57047
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Breast Carcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
B7H4 is a glycosylated transmembrane protein of the B7 family. It binds to activated T cells to moderate the T cell responses via cell cycle arrest in the T cell. Reverse signaling can induce either cell cycle arrest or apoptosis in the B7H4 expressing cell. B7H4 is up-regulated in several carcinomas in correlation with tumor progression and metastasis. A soluble form of B7H4 is elevated in the serum of ovarian cancer, renal cell carcinoma, and rheumatoid arthritis patients, also in correlation with advanced disease status.
Serine/Threonine-Protein Kinase B-Raf (BRAF) is a cytoplasmic serine-threonine kinase of the RAF family, which mediates downstream cellular responses to growth signals through the mitogen-activated protein kinase (MAPK) signaling pathway. Oncogenic mutations in the BRAF gene, 80% of which are a single V600E substitution within the kinase domain, constitutively activate the MAPK signaling pathway and result in increased cell proliferation and apoptosis resistance. The V600E mutation is observed in colorectal cancer, non-Hodgkin's lymphoma, papillary thyroid carcinoma, malignant melanoma, non-small-cell lung carcinoma, and lung adenocarcinoma. BRAF V600E is therefore an important immunohistochemical marker for tumour diagnosis and prognosis.
Isocitrate Dehydrogenase 1 (IDH1) is a soluble, cytoclic enzyme involved in the TCA metablic cycle. The most notable mutation in this enzyme, R132H, is clinically indicated in the majority of astrocytomas and oligodendroglial tumours, with the mutation being associated with more favourable prognosis and increased survival in those patients. IDH1 R132H is also useful in the differential diagnosis between anaplastic glioma and glioblastoma.
Hepatocyte Specific Antigen, also known as Hep-Par1, has proven to be strongly useful in the detection of both benign and malignant liver-derived tissues, and associated tumours such as hepatoblastoma and hepatocellular carcinoma (HCC). The pathological diagnosis of HCC is often difficult as it shares histological and cytological features with adenoid cystic carcinoma, renal cell carcinoma, adenocarcinoma, and cholangiocarcinoma. Hep-Par1 is indicated as an effective marker to distinguish between these mimics, and therefore aids in the differential diagnosis of HCC.
BOB-1 is a B-cell-specific coactivator whose expression majorly restricted to mature B-cells, and typically in germinal center B-cells. It is co-activator for Oct-1 and Oct-2 transcription factors. BOB-1 is used for B-lineage determination of CD20- plasmablastic or diffuse large B-cell lymphoma (DLBCL). BOB-1 was also presented to be useful marker when combined together with CD79a and Cyclin E for discriminating classical Hodgkins lymphoma from primary mediastinal large B-cell lymphoma.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC060
GMDN Code:
62794
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Tonsil
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
The Her3 [IHC113] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissues within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Applications:
IHC
Clone number:
IHC113
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Breast Cancer
Buffer:
Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
Cluster of Differentiation 71 (CD71), also known as Transferrin Receptor Protein 1 (TfR1) or the transferrin receptor, is a cell surface proliferation marker that is involved in the cellular uptake of iron. CD71 is most highly expressed in early erythroid precursors and is fully absent from mature erythrocytes; CD71 is therefore highly useful as a marker for erythroid components within bone marrow biopsy specimens, without interference from mature erythrocytes. CD71 expression has been indicated in invasive breast carcinoma with acquired resistance to tamoxifen, and has been linked to poor prognosis in ER+/luminal-like breast cancer. Anti-CD71 is used in the determination of erythroid leukemia, benign erythroid proliferative disorders, and myelodysplastic syndrome.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC071
Antibody Isotype:
IgG1, kappa
GMDN Code:
57014
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Bone Marrow
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Cluster of Differentiation 56 (CD56), also known as Neural-Cell Adhesion Molecule (NCAM), is a glycoprotein involved in synaptic plasticity, cell-cell adhesion, neurite outgrowth, learning, and memory. NCAM is expressed in normal neurons, glia, natural killer cells, activated T-cells, brain and cerebellum, neuroendocrine tissues, and skeletal muscle. Anti-CD56 recognizes a number of tumours including myeloma, myeloid leukemia, natural killer/T-cell lymphomas, neuroendocrine tumours, pancreatic acinar-cell carcinoma, pheochromocytoma, and Wilm's tumour. CD56 is detectable in neoplasms that are neuroectodermally-derived, such as retinoblastoma, medulloblastomas, astrocytomas, small cell carcinomas, and neuroblastomas. It has also been linked to rhabdomyosarcoma, a tumour that is mesodermally-derived.
CA-125 is normally found in epithelial cells of Fallopian tube, endometrium and endocervix, pancreas, colon, gall bladder, stomach, kidney, apocrine sweat gland, mammary gland, and mesothelial cell lining of pleura, pericardium, and the peritoneum. Anti-CA-125 reacts positively with ovarian malignancies, cervical carcinoma, seminal vesicle carcinoma, anaplastic lymphoma, and endometrial and bladder adenocarcinoma.
Cluster of Differentiation 68 (CD68) is a heavily glycosylated transmembrane antigen that is detected in lysosomes, tissue macrophages, Langerhans cells, dendritic cells, monocytes, Kupffer cells, osteoclasts, and granulocytes. Anti-CD68 may be useful in identifying myelomonocytic and histiocytic tumours, and for differentiating between malignant fibrous histiocytoma and other pleomorphic sarcomas. However, other lysosome-rich cells may also stain, since Anti-CD68 detects a formalin-resistant epitope that may be associated with lysosomal granules.
β-Catenin is a cytoplasmic protein with a dual role in cell-cell adhesion and gene expression. It is normally present in the submembranous regions of the cell, and nuclear accumulation of β-Catenin has been found to occur as a result of gene mutations. This accumulation is useful in identifying desmoid tumours (fibromatosis) in the abdomen and breast, and is therefore useful in differentiating other cell neoplasms in these regions.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC516
Antibody Isotype:
IgG1
GMDN Code:
56911
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Fibromatosis of Breast
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Anaplastic Lymphoma Kinase (ALK) is a receptor tyrosine kinase which plays a role in brain and nervous system development. ALK is typically expressed at low levels in regions of the developing central and peripheral nervous system, such as the neonatal brain and spinal cord. The most common genetic alterations of this gene are chromosomal translocations, which result in multiple ALK fusion proteins that are involved in tumourigenesis, as in the case of anaplastic large cell lymphoma (ALCL), lung adenocarcinoma, and inflammatory myofibroblastic tumours. Aberrant ALK expression is also found in other tumours such as familial neuroblastoma, non-small cell lung carcinoma (NSCLC), and brain cancers.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC509
Antibody Isotype:
IgG2b
GMDN Code:
56791
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Anaplastic Large Cell Lymphoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
B-Cell Lymphoma 6 (BCL6) is a zinc finger transcription factor. BCL6 expression is seen in follicular lymphomas, Burkitt's lymphoma, angioimmunoblastic T-cell lymphoma, and nodular lymphocyte-predominant Hodgkin's lymphoma. Together with BCL2, BCL6 is often used to distinguish neoplastic follicles from those in benign hyperplasia, and to aid in the classification of mantle cell lymphomas and nodular lymphocyte-predominant Hodgkin's lymphoma.
Alpha-Fetoprotein (AFP) is a major plasma glycoprotein seen in hepatocytes of fetal liver and in hepatoma. Elevated levels of AFP in adult serum may be indicative of hepatocellular carcinoma, hepatoid adenocarcinoma, germ cell tumours, or yolk sac tumours. In hepatocellular carcinoma, AFP expression usually indicates malignancy in a hepatocellular nodule and hepatic histogenesis of a malignancy.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC714
Antibody Isotype:
IgG
GMDN Code:
56770
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Hepatocellular Carcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Stathmin regulates microtubule dynamics in the cell cycle. It is present in all tissues, but is mostly pronounced in constantly proliferating cell types. Anti-Stathmin staining has been found to correlate with cervical intraepithelial neoplasia (CIN) grade, with CIN 3 presenting the greatest expression and CIN 1 displaying the least expression of stathmin.
The HBcAg [IHC205] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of chronic HBV infection tissue within the context of antibody panels, the patients clinical history and other diagnositc tests as evaluated by a qualified pathologist.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Applications:
IHC
Clone number:
IHC205
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Liver infected with Hepatitis B virus
Buffer:
Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
DOG1, also known as Discovered on GIST-1, is a marker that is highly specific for gastrointestinal stromal tumour (GIST). Anti-DOG1 is extremely sensitive for the detection of GIST and its diagnosis. Although some GIST stain weakly for c-kit, DOG1 is expressed in the vast majority of GIST cases. Reports have also indicated DOG1 as a marker for salivary acinar and intercalated duct differentiation.
Collagen Type IV is a primary component of the basal lamina that is used as a marker to observe the presence of the lamina and examine its structure. In addition to the epithelial basal lamina, Anti-Collagen Type IV stains mesenchymal components. It is useful for identifying soft tissue cancers, including schwannomas and leiomyomas. Anti-Collagen Type IV frequently reacts with these tissues after becoming well-differentiated and malignant. The use of Anti-Collagen Type IV produces more reliable results than non-specific silver reticulum stains when investigating the vascular elements of neoplasms, hemangiopericytoma, angiosarcoma, and epithelioid hemangioendothelioma.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC549
Antibody Isotype:
IgG1, kappa
GMDN Code:
57056
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Lung, Muscle
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Anti-Hairy Cell Leukemia stains various B-cells in the follicular mantle zone and virtually all cases of hairy cell leukemia. It also stains some high grade B-cell lymphomas.
COX-2, also known as Cyclooxygenase 2, catalyzes the conversion of arachidonic acid to prostaglandin H2. The inhibition of COX-2 using non-steroidal anti-inflammatory agents limits angiogenesis and tumour growth, and increases apoptosis. The overexpression of COX-2 is linked to increased microvascular density.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC081
GMDN Code:
57064
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Colon Adenocarcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Growth Hormone (GH or hGH) is a peptidic hormone produced by somatotrophs of the anterior pituitary gland. Anti-Growth Hormone stains somatotrophs in normal pituitary tissues, and is useful in identifying pituitary tumours and understanding pituitary disease or acromegaly. Studies have also found Anti-GH to stain non-pituitary cells, such as hepatocellular carcinoma and cutaneous lesions.
Survivin is an apoptosis inhibitor that is nearly undetectable in terminally differentiated cells, but found in most tumours including renal cell carcinoma, ovarian carcinoma, hepatocellular carcinoma, prostate carcinoma, and breast carcinoma. Survivin expression is linked to tumour progression, but not patient survival.
Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA-4) is a receptor on T helper cells that functions as an immune checkpoint and downregulator of immune responses. Mutations in CTLA-4 are associated with insulin-dependent diabetes mellitus, Hashimoto's thyroiditis, Graves' disease, systemic lupus erythematosus (SLE), celiac disease, primary biliary cirrhosis, thyroid-associated orbitopathy, multiple sclerosis, and other autoimmune diseases. The spliced variant of CTLA-4 in SLE is present in the patient's serum. Haploinsufficiency of CTLA-4 causes the immune system disorder known as CTLA-4 deficiency or CHAI disease (CTLA-4 haploinsufficiency with autoimmune infiltration).
Glypican-3 (GPC3) is a GPI-anchored proteoglycan involved in cell division and growth regulation. Glypican-3 is a useful tumour marker, and its expression has been shown to be upregulated in hepatocellular carcinoma (HCC), hepatoblastoma, melanoma, testicular germ cell tumours, and Wilms' tumour. Patients with HCC have presented elevated levels of GPC3 in the neoplastic liver tissues and serum, levels which are higher than detected in cirrhotic liver or liver with focal lesions, including those with hepatic adenoma and dysplastic nodules. Glypican-3 is also overexpressed in testicular germ cell tumours of certain subtypes, such as yolk sac tumours and choriocarcinoma, and in embryonal tumours.
Desmoglein-3 Antibody (DSG3) is a component of desmosomes in vertebrate epithelial cells. It identifies pulmonary squamous cell carcinomas from other types of lung cancer with a highly specificity and sensitivity. Studies show the upregulation of DSG3 correlated with metastasis in a number of cancers including lung cancers. The expression of DSG3 indicates a poor prognosis and portends a more aggressive clinical outcome.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC083
Antibody Isotype:
IgG1
GMDN Code:
?
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Skin Cancer
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Glycophorin A (GPA) and Glycophorin B (GPB) are erythrocyte blood group determinants that minimize erythrocyte aggregation during the circulation of blood. Anti-Glycophorin A is useful for understanding erythroid cell development and identifying erythroid leukemias.
Glutamine Synthetase (GS-6 or GS) catalyzes the conversion of glutamate and ammonia to glutamine in the liver, and is expressed in pericentral hepatocytes, but not in periportal hepatocytes or in the mid-zonal. Anti-Glutamine Synthetase is useful in some hepatocellular carcinomas and many high grade dysplastic nodules, and therefore may be useful in recognizing these cases. A panel of antibodies against HSP70 (heat shock protein 70), GPC3, and glutamine synthetase is useful for differentiating dysplastic from early malignant hepatocellular nodules in cirrhosis. GS staining of hepatocellular lesions is useful for the differential diagnosis of focal nodular hyperplasia (FNH), hepatic adenoma (HCA), dysplastic nodules, and low grade hepatocellular carcinoma. FNH produces a “map-like” pattern when stained with Anti-Glutamine Synthetase. Conversely, HCA can stain negatively, produce border staining, or stain around the tumour veins.
Synaptophysin is a synaptic vesicle glycoprotein used in synaptic transmission of neurons. Anti-Synaptophysin stains the gastrointestinal mucosa and lung neuroendocrine cells of the human adrenal medulla, carotid body, pancreas, pituitary, skin, and thyroid. Synaptophysin also stains neuroendocrine neoplasms. Use of Anti-Synaptophysin produces diffuse, finely granular, cytoplasmic staining. The presence of synaptophysin does not correlate with neuron-specific enolase or other neuroendocrine markers.
Cyclin D1 is an essential cell cycle regulator and proto-oncogene. Cyclin D1 staining is useful for investigating cell cycle biology and related cancers. Anti-Cyclin D1 is used for differentiating mantle cell lymphomas (positive stain) from CLL/SLL and follicular lymphomas (negative stain). Hairy cell leukemia and plasma cell myeloma also react lightly to Anti-Cyclin D1.
Glucose transporter type I (GLUT1), also known as SCL2A1, is a glucose transporter present in the blood-brain barrier and erythrocytes. GLUT1 overexpression is associated with tumour progression or poor prognoses of bladder, breast, cervical, colon, and lung carcinomas, as well as mesothelioma. Anti-GLUT1 is useful for distinguishing malignant mesothelioma (GLUT1(+)) from reactive mesothelium (GLUT1(-)).
Tumour-Associated Glycoprotein 72 (TAG-72) is a glycoprotein found on the surface of many cancer pathologies. Anti-TAG-72 can be useful for detecting some adenocarcinomas and non-neoplastic tissues. This diagnostic grade TAG-72 IVD antibody is useful for identifying adenocarcinomas in positive staining, but in mesotheliomas no staining is observed.
Glial Fibrillary Acidic Protein (GFAP) is an intermediate filament protein that is present in astrocytes and some ependymal cells of the central nervous system. In the peripheral nervous system, GFAP is present in Schwann cells, enteric glial cells, and satellite cells. Anti-GFAP staining is useful in differentiating neoplasms of astrocyte origin from other neoplasms in the central nervous system.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC584
Antibody Isotype:
IgG
GMDN Code:
57238
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Brain
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
GATA3 is a transcription factor important in cell proliferation, development, and differentiation. GATA3 is mostly observed in breast and urothelial carcinomas, and is rarely present in other cancers such as endometrial endometrioid adenocarcinoma. Among the breast carcinomas, GATA3 has a lower expression in luminal B subtype breast carcinoma. Studies have found GATA3 expression to be associated with ER (estrogen receptor), PR (progesterone receptor), and HER2 in breast cancer cases. Urothelial carcinomas stain positively for GATA3 in invasive or high grade tumours, therefore Anti-GATA3 is useful for carcinoma diagnosis when those of the breast and bladder are plausible.
Desmin is a type III intermediate filament present in normal smooth, skeletal, and cardiac muscle cells. Analysis by light microscopy suggests desmin localizes towards the periphery of Z-lines in striated muscle fibrils. Desmin connects cytoplasmic dense bodies to membranous dense plaques in smooth muscles. Anti-Desmin stains rhabdomyomas, leiomyosarcoma, rhabdomyosarcoma, leiomyomas, and perivascular cells from skin glomus tumours, and is used to identify the myogenic characteristics of tumours. Desmin can also be found in myofibroblasts and desmoid fibromatosis.
Galectin-3 is a lectin involved in cell adhesion, macrophage activation, angiogenesis, metastasis, and apoptosis. Anti-Galectin-3 is useful for distinguishing between benign and malignant thyroid neoplasms. Galectin-3 is also useful for recognizing anaplastic large cell lymphoma.
Thyroid Transcription Factor 1 (TTF-1) is present in diencephalon, lung, and thyroid. Anti-TTF-1 stains thyroid and thyroid-derived tumours, and is therefore used for distinguishing lung adenocarcinoma from germ cell tumours, malignant mesothelioma, and metastatic carcinomas from organs other than the thyroid. It is also useful for distinguishing small cell lung carcinoma from lymphoid infiltrates, and pulmonary from non-pulmonary adenocarcinomas in malignant effusions. The ability to distinguish between pulmonary and non-pulmonary adenocarcinomas is particularly useful in identifying tumours that have metastasized to the brain.
The Cytokeratin 10 [IHC135] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissue within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Applications:
IHC
Clone number:
IHC135
UKCA Status:
UKCA
CE-IVD Status:
RUO
Buffer:
Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
Cytokeratin 14 (CK14) is found in squamous epithelial basal cells, myoepithelium, some glandular epithelia, and mesothelial cells. Anti-Cytokeratin 14 is useful for distinguishing squamous cell carcinomas from other epithelial tumours, and for classifying metaplastic breast carcinomas.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC555
Antibody Isotype:
IgG3, kappa
GMDN Code:
57079
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Squamous Cell Carcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Vimentin is a component of intermediate filament in mesenchymal cells, such as endothelial cells, fibroblasts, lymphocytes, and melanocytes. Anti-Vimentin is useful for assessing whether tissue samples have been processed and preserved properly. A panel of Anti-Vimentin and Anti-Keratin is useful for differentiating melanomas from large cell lymphomas and undifferentiated carcinomas. This diagnostic grade Vimentin IVD antibody stains melanomas and schwannomas, as well as endometrial endometrioid adenocarcinomas.
Follicle-Stimulating Hormone (FSH) allows for progression of ovarian folliculogenesis, and enables Sertoli cell proliferation in the testis. Anti-FSH reacts with FSH-producing cells, and therefore FSH staining is useful for classifying pituitary cancers and understanding pituitary disease.
Cytokeratin 18 (CK18) is present in simple, glandular, and transitional epithelial cells, but is absent in stratified epithelial cells. CK18 usually multimerizes with Cytokeratin 8, and Anti-Cytokeratin 18 is useful for detecting adenocarcinomas of simple and glandular epithelium origin, as well as poorly differentiated squamous carcinoma cells.
Podoplanin is a transmembrane mucoprotein specifically expressed in the endothelium of lymphatic capillaries, while remaining absent from the blood vasculature. The protein is co-localized with VEGFR3/FLT4 in normal skin and kidney. Anti-Podoplanin is useful in the identification of lymphangiomas, Kaposi's sarcomas, epithelioid mesotheliomas, hemangioblastomas, seminomas, and some angiosarcomas that likely have lymphatic differentiation.
Cytokeratin 19 (CK19) forms intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue and provides mechanical support. Anti-Cytokeratin 19 stains epithelia and epithelial malignancies such as carcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Cytokeratin 19 is a useful marker for distinguishing hepatocellular carcinoma from intrahepatic cholangiocarcinoma. This differentiation is improved when stained in combination with Cytokeratin 7, CAM5.2, Ber-EP4/MOC31, Hep-Par1, and TTF1. Cytokeratin 19 staining can also be used to recognize thyroid papillary carcinomas.
Postmeiotic Segregation Increased 2 (PMS2) is a DNA repair protein involved in mismatch repair. Mutations and deficiencies in the PMS2 gene have been linked to microsatellite instability and malignancies such as hereditary nonpolyposis colorectal cancer and endometrial cancer. Expression levels of the PMS2 protein may be useful as a screening tool for Lynch syndrome after a colorectal cancer diagnosis. Anti-PMS2 is recommended to be used as part of a panel along with antibodies against MSH2, MSH6, and MLH1.
Cytokeratin 20 (CK20) forms intermediate filaments and is normally present in gastric and intestinal epithelium, urothelium, and Merkel cells. Anti-Cytokeratin 20 is used for distinguishing specific types of urinary tract epithelial cells and malignant epithelia. Anti-Cytokeratin 20 stains tissues of the colon, stomach, pancreas, biliary system adenocarcinomas, transitional-cell, mucinous ovarian tumours, and Merkel cell carcinomas. Non-mucinous tumours of the ovary and adenocarcinomas of the breast, lung, endometrium, squamous cell, and small cell type are not stained by Anti-Cytokeratin 20.
LMO2, also known as LIM-Only Transcription Factor 2, RBTN2, or TTG2, is an oncoprotein that is expressed in normal germinal center B-cells, as well as bone marrow hematopoietic precursors and endothelial cells. LMO2 plays a role in angiogenesis and hematopoesis, and its expression has been detected in erythroid and myeloid precursors, megakaryocytes, and also in lymphoblastic and acute myeloid leukemias. LMO2 protein expression has been noted in diffuse large B-cell lymphoma, the most common adult non-Hodgkin's lymphoma, as well as follicular lymphoma, a neoplasm derived from germinal center B-cells that accounts for a number of cases of non-Hodgkin's lymphomas.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC615
Antibody Isotype:
IgG1, kappa
GMDN Code:
63898
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Tonsil, Follicular Lymphoma, Diffuse Large B-Cell Lymphoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Parathyroid Hormone (PTH), also known as Parathormone or Parathyrin, is a hormone secreted by the parathyroid glands that functions to increase the concentration of calcium in the blood. Anti-Parathyroid Hormone (PTH) is useful for differentiating parathyroid hyperplasia/neoplasms from thyroid and metastatic neoplasms, and is also used in the consideration of parathyroid carcinomas located primarily in the anterior mediastinum.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC645
Antibody Isotype:
IgM
GMDN Code:
63169
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Parathyroid Tissue
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
p63 is a tumour suppressor protein that is very similar to p53 in structure and function, while being homologous to p73. p63 is important in development and differentiation, and has been identified as a useful marker for distinguishing between lung squamous cell carcinomas and adenocarcinomas. Anti-p63 is also used to differentiate between benign and malignant prostate and breast lesions, due to its labeling of the nuclei of myoepithelial cells in both tissue types.
p57<sup>Kip2</sup>, also known as p57, is a tumour suppressor protein that causes cell cycle arrest at G1 by binding to G1 cyclin-CDK complexes. The p57<sup>Kip2</sup> gene is a potential tumour suppressor target as the gene is located in a chromosomal region implicated in sporadic cancers, Wilms' tumour, and Beckwith Wiedemann syndrome. Anti-p57<sup>Kip2</sup> labels many cytotrophoblast nuclei and stromal cells in normal placenta, and is useful in differentiating between complete hydatidiform mole and partial hydatidiform mole or hydropic abortion.
Transcription Factor E3 (TFE3) is a transcription factor that binds to the MUE3-type E-box sequences involved in TGF-β signaling. Anti-TFE3 staining is the most sensitive and specific indicator of Xp11 translocation renal cell carcinomas. Since alveolar soft part sarcoma (ASPS) is characterized by a specific chromosomal rearrangement resulting in a chimeric transcription factor (ASPSCR1-TFE3), this TFE3 IVD antibody is also a useful diagnostic tool for recognizing ASPS.
Calretinin is a calcium-binding protein that functions as a modulator of neuronal excitability and may play a protective role in the survival of nerve cells during disturbances in calcium homeostasis. It is abundantly expressed in subsets of neurons throughout the brain and spinal cord, particularly retina and sensory ganglia, but it is also found in mesothelium, eccrine sweat glands, Sertoli cells, ovarian stromal cells, and adrenal cortical cells. Due to its high sensitivity against mesothelial cells, calretinin is a useful marker in differentiating mesothelioma and metastatic adenocarcinoma to the serous membranes. It is also a diagnostic marker of Hirschsprung's disease and some ovarian and testicular cancers such as Sertoli-Leydig cell tumour, Sertoli cell tumour, Leydig cell tumour, sex cord tumour with annular tubules, and steroid cell tumour.
p53, also known as Tumour Protein 53 or TP53, is a tumour suppressor and transcription factor that functions in a number of anti-cancer activities including DNA repair, cell-cycle arrest, and apoptosis in response to DNA damage or other stressors. Mutations in p53 are linked to a number of malignant tumours, including those of the breast, ovary, bladder, colon, lung, and melanoma. Anti-p53 staining has been used to detect intratubular germ cell neoplasia, and also to distinguish between uterine serous carcinoma and endometrioid carcinoma.
Prostate-Specific Antigen (PSA) is a serine protease of the kallikrein family that is produced by the prostate epithelium and epithelial lining of the periurethral glands. Although considered prostate-specific, PSA has also been detected in breast tissue, breast tumours, endometrium, adrenal neoplasms, and renal cell carcinomas. Anti-PSA can be used for differentiating high-grade prostate adenocarcinoma from high-grade urothelial carcinoma, as well as for determining the prostatic origin of carcinomas in non-prostate tissues. Anti-PSA recognizes primary and metastatic prostatic neoplasms, but not tumours of nonprostatic origin, and can be useful as an aid to confirm prostatic acinar cell origin in primary and metastatic carcinomas.
p504s, also known as ?-Methylacyl Coenzyme A Racemase (AMACR), is an enzyme localized in the peroxisome and mitochondria that functions in ?-oxidation of branched chain fatty acids, as well as bile synthesis. AMACR has been clinically indicated as a tissue biomarker for prostate cancer and colorectal cancer, as well as high-grade prostatic intraepithelial neoplasia, a precursor lesion of prostate cancer. p504s overexpression has also been detected in a number of other cancers including ovarian, breast, bladder, lung, and renal cell carcinomas, lymphoma, and melanoma.
Flt-1, also known as Fms Related Tyrosine Kinase 1 or VEGFR1 (Vascular Endothelial Growth Factor Receptor 1), is a tyrosine kinase involved in lymphangiogenesis, angiogenesis, and wound healing. It is present in endothelial cells, osteoblasts, placental trophoblasts, renal mesangial cells, and some hematopoietic stem cells. Anti-Flt-1/VEGFR1 is useful for identifying carcinomas of the larynx and esophagus.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC086
GMDN Code:
?
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Angiosarcoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Cluster of Differentiation 10 (CD10) is a cell surface metalloendopeptidase that cleaves and inactivates several peptide hormones including glucagon, enkephalins, and oxytocin. Also known as Common Acute Lymphoblastic Leukemia Antigen (CALLA), it is an important cell surface marker in the diagnosis of human ALL (Acute Lymphocytic Leukemia), and is found positive in precursor B lymphoblastic leukemia/lymphoma, angioimmunoblastic T-cell lymphoma, Burkitt's lymphoma, and follicular germinal center lymphoma. CD10 expression has also been reported in a variety of non-hematolymphoid tissues, particularly of the kidney. It is a useful aid in the diagnosis of various malignant tumours such as renal cell carcinoma, endometrial stromal sarcoma, and hepatocellular carcinoma.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC525
Antibody Isotype:
IgG1
GMDN Code:
56938
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Kidney, Lymph Node, Tonsil
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Anti-p40 recognizes squamous and basal cells, the shortest variant of p53, and ΔNp63 (an isoform of p63). p40 has been indicated as an alternative to p63 for the detection of Squamous Cell Carcinoma (SqCC), offering the advantage of eliminating potential misinterpretation of a positive adenocarcinoma as a SqCC.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC058
Antibody Isotype:
IgG1
GMDN Code:
64957
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Squamous Cell Carcinoma of Lung
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
CD117 or Proto-oncogene c-Kit (c-Kit) is a member of the Tyrosine Kinase Receptor (TKR) family, and is an important cell surface marker found on hematopoietic stem cells, melanocytes, mast cells, Cajal cells, germ cells, basal cells of skin, and mammary ductal epithelia. It is considered an important marker in the diagnosis and classification of Gastrointestinal Stromal Tumours (GISTs), mast cell diseases, Acute Myeloid Leukemia (AML), Small Cell Lung Carcinoma (SCLC), and Ewing's sarcoma.
p27, also known as p27<sup>Kip1</sup>, is a cyclin-dependent kinase inhibitor that binds to and inhibits cyclin-dependent kinases, thereby regulating progression from G1 to S phase. Decreased expression of p27 is linked to poor prognosis in renal-cell carcinoma, colon carcinoma, small breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, lymph node metastases in papillary carcinoma of the thyroid, and is associated with a more aggressive phenotype of carcinoma in the cervix.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC128
GMDN Code:
57500
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Tonsil
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Cluster of Differentiation 13 (CD13) is a transmembrane protein that is overexpressed in both hematological and solid malignancies, including Acute Myeloid Leukemia (AML). Although hypogranular variants of AML are difficult to distinguish from other AML subtypes due to the morphology, the diagnosis of this variant is possible through using a panel of CD13, CD16, CD33, CD34, and CD117. Alternatively, a panel of CD13, CD34, CD43, CD68, CD117, CD163, lysozyme, and MPO is very useful for accurately diagnosing myeloid sarcoma and distinguishing it from large cell lymphoma, undifferentiated carcinoma, lymphoblastic lymphoma, malignant melanoma, Burkitt's lymphoma, extra-medullary hematopoiesis, and inflammation. Since CD13 is expressed in both normal and neoplastic liver tissues, CD13 staining is useful for distinguishing between hepatocellular carcinoma and non-hepatocellular neoplasms.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC123
Antibody Isotype:
IgG1
GMDN Code:
62795
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Liver
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
p21, also known as p21^ Cip1 ^ , p21^ Waf1 ^, Cyclin-Dependent Kinase Inhibitor 1, or CDK-Interacting Protein 1, functions to regulate cell cycle progression at G1 by inhibiting the activity of Cyclin-CDK2 or -CDK4 complexes. This cyclin-dependent kinase inhibitor is expressed in all adult human tissues, and decreased expression of p21 is linked to poor prognosis in a number of carcinomas including gastric carcinoma, non-small cell lung carcinoma, and thyroid carcinoma. p21 is also associated with favourable prognosis in several tumours.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC021
Antibody Isotype:
IgG1, kappa
GMDN Code:
57493
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Tonsil
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Cluster of Differentiation 13 (CD13) is a transmembrane protein that is overexpressed in both hematological and solid malignancies, including Acute Myeloid Leukemia (AML). Although hypogranular variants of AML are difficult to distinguish from other AML subtypes due to the morphology, the diagnosis of this variant is possible through using a panel of CD13, CD16, CD33, CD34, and CD117. Alternatively, a panel of CD13, CD34, CD43, CD68, CD117, CD163, lysozyme, and MPO is very useful for accurately diagnosing myeloid sarcoma and distinguishing it from large cell lymphoma, undifferentiated carcinoma, lymphoblastic lymphoma, malignant melanoma, Burkitt's lymphoma, extra-medullary hematopoiesis, and inflammation. Since CD13 is expressed in both normal and neoplastic liver tissues, CD13 staining is useful for distinguishing between hepatocellular carcinoma and non-hepatocellular neoplasms.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC119
Antibody Isotype:
IgG1
GMDN Code:
56944
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Liver
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
The p16 (p16<sup>INK4A</sup>) protein is a cyclin-dependent kinase (CDK) inhibitor that plays an important regulatory role in the cell cycle. By controlling the transition between the G1 and S phases through regulation of retinoblastoma protein, p16 decelerates cellular differentiation and therefore acts as a tumor suppressor, making it the key marker in several human cancers including head and neck cancer, perianal lesions, melanomas, gliomas, lymphomas, and some types of leukemia. p16 is also clinically indicated in carcinomas of the esophagus, pancreas, lung, biliary tract, liver, colon, and urinary bladder.
Cluster of Differentiation 138 (CD138), also known as Syndecan-1, is a trans- membrane glycoprotein present on the surface of B-cells during late stage differentiation. Anti-CD138 is used to differentiate marginal zone lymphoma from lymphoplasmacytic lymphoma. ALK+ Large B-Cell Lymphoma (LBCL) commonly stains positively for CD138, but not for CD20 and CD79a. Anti-CD138 reacts positively with HHV8-associated primary effusion lymphoma that lacks B-cell markers. CD138 is also a useful marker for identifying and enumerating benign, reactive, or malignant plasma cells from the bone marrow biopsy samples.
The p16 (p16<sup>INK4A</sup>) protein is a cyclin-dependent kinase (CDK) inhibitor that plays an important regulatory role in the cell cycle. By controlling the transition between the G1 and S phases through regulation of retinoblastoma protein, p16 decelerates cellular differentiation and therefore acts as a tumor suppressor, making it the key marker in several human cancers including head and neck cancer, perianal lesions, melanomas, gliomas, lymphomas, and some types of leukemia. p16 is also clinically indicated in carcinomas of the esophagus, pancreas, lung, biliary tract, liver, colon, and urinary bladder.
The CD14 [IHC014] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissues within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
p120 Catenin is a nucleolar protein belonging to the armadillo protein family, which is involved in cell-cell adhesion and signal transduction. p120 Catenin is associated with proliferation, and is found in the majority of human malignant tumours, while remaining absent from resting normal cells. Anti-p120 Catenin is useful in differentiating between ductal and lobular neoplasia in the breast, and strong staining with Anti-p120 Catenin is associated with discohesive infiltrative morphology in gastric and colonic carcinoma. Accumulation of p120 Catenin in the cytoplasm has been linked to lung cancer, pancreatic cancer, and gastric cancer, and is correlated to poor prognosis in colon cancer.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC120
Antibody Isotype:
IgG1
GMDN Code:
62843
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Lobular Breast Carcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Prostatic Specific Acid Phosphatase (PSAP) is a prostatic enzyme found in the glandular epithelium of the prostate. PSAP levels are elevated in hyperplastic prostate and prostate carcinoma, with the highest levels being detected in metastasized prostate cancer. Moderate overexpression of PSAP is also characteristic of diseases of the bone (such as Paget's disease or hyperparathyroidism), diseases of blood cells (such as sickle-cell disease), multiple myeloma, or lysosomal storage diseases (such as Gaucher's disease). PSAP is considered more sensitive, yet less specific, than PSA, however Anti-PSAP can act as a useful complement to Anti-PSA under suitable clinical contexts.
Cluster of Differentiation 15 (CD15), also known as Leu-M1, is a carbohydrate adhesion molecule. Positive staining for CD15 and negative staining for leukocyte common antigen or other B- or T-cell lineage markers helps recognize Reed Sternberg cells (RSC) in classic Hodgkin's lymphoma, and distinguishes it from Hodgkin-like neoplasms. CD15 does not stain mesotheliomas and is therefore most useful for distinguishing epithelial mesothelioma from adenocarcinoma.
OX40 is a member of the tumor necrosis factor (TNF) receptor superfamily, which regulates T cell activity and immune response. OX40 is mainly activated by CD4 + and CD8 + T cells. It is expressed on cells, while OX40 ligands (OX40L, TNFSF4, CD252) are mainly expressed on activated antigen-presenting cells. Research shows that OX40 pathway is related to inflammation and autoimmune diseases. Other studies have shown that OX40 agonists can enhance the anti-tumor immunity of several cancer types.
Cluster of Differentiation 16 (CD16) is a receptor on natural killer cells, neutrophils, monocytes, and macrophages. CD16 binds the Fc portion of antibodies to activate these immune cells. CD16 staining is useful in the differential diagnosis of hepatosplenic gamma delta T-cell lymphoma and gamma delta T-cell large granular lymphocyte leukemia from mucosal and cutaneous gamma delta T-cell lymphoma. Likely due to dysgranulopoiesis, granulocytes with myelomonocytic leukemia have decreased CD16 expression in comparison to granulocytes with chronic myelogenous leukemia and control bone marrow biopsies.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC528
Antibody Isotype:
IgG1
GMDN Code:
56950
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Liver
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
OX40 is a member of the tumor necrosis factor (TNF) receptor superfamily, which regulates T cell activity and immune response. OX40 is mainly activated by CD4 + and CD8 + T cells. It is expressed on cells, while OX40 ligands (OX40L, TNFSF4, CD252) are mainly expressed on activated antigen-presenting cells. Research shows that OX40 pathway is related to inflammation and autoimmune diseases. Other studies have shown that OX40 agonists can enhance the anti-tumor immunity of several cancer types.
Fibronectin is a glycoprotein that contributes to cell adhesion, migration, and metastasis. Renal cancer cells exhibit higher expression of fibronectin, therefore Anti-Fibronectin is useful for assessing the progression and aggressiveness of renal cancer cells.
Octamer-Binding Transcription Factor 4 (Oct-4), also known as POU5F1 (POU Domain, Class 5, Transcription Factor 1), is a member of the POU homeodomain family of transcription factors and is involved in the maintenance and regulation of pluripotency in embryonic stem and germ cells. Anti-Oct-4 is highly useful and sensitive for seminomas, germinoma, dysgerminoma, embryonal carcinoma, and gonadoblastoma. Oct-4 may be associated with tumourigenesis, and can have an effect on some aspects of tumour behavior, including tumour recurrence or resistance to therapies.
TIGIT is an immune receptor present on some T cells and Natural Killer cells. TIGIT binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. Through the CD226/TIGIT-PVR pathway, TIGIT regulates T cell mediated immunity. In cancer, TIGIT and PD-1 have been shown to be over-expressed on tumor antigen-specific CD8+ T cells and CD8+ tumor infiltrating lymphocytes (TILs) from individuals with melanoma. Blockade of TIGIT and PD-1 led to increased cell proliferation, cytokine production, and degranulation of tumor antigen-specific CD8+ T cells and TIL CD8+ T cells. It can be considered an immune checkpoint.
Cluster of Differentiation 163 (CD163) is a receptor found exclusively on the surface of monocytes and macrophages. The solubilized form in plasma is upregulated in inflammatory diseases such as rheumatoid arthritis, atherosclerosis, and Gaucher’s disease, which supports recent studies that have found IL-10, glucocorticoids, and other inflammatory modulators to upregulate CD163 expression. CD163 staining is useful for differentiating synovial intimal fibroblasts from synovial macrophages in rheumatoid arthritis. Overexpression of CD163 is also present in patients with myelomonocytic leukemia dealing with microbial infections. CD163 expression is found in leukemias with monocytic differentiation and synovial-type giant cell tumours of the vertebral column.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC163
Antibody Isotype:
IgG1
GMDN Code:
62796
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Inflamed Tissue
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Cluster of Differentiation 19 (CD19) is a surface receptor found on follicular dendritic cells and B-cells. CD19 is found on normal and malignant B-cells, and is known as a reliable marker for B-cells throughout its maturation stages. Anti-CD19 reacts positively with the mantle zone cells, scattered cells, and germinal centers of normal lymph tissues. Although CD20 and CD22 have similar staining patterns to CD19, CD19 is useful because it is also expressed in immature B-cells.
Neurotrophic tyrosine kinase receptor (NTRK) is a family of 3 proto-oncogenes including NTRK1, NTRK2, and NTRK3. NTRK gene fusions have been reported in a variety of tumor types, which are involved in biological processes such as neuronal survival, differentiation, and plasticity under physiological circumstances. Recently, FDA has approved ENtrectinib for patients with NTRK fusions, thus testing for NTRK fusions identifies patients who may be candidates for NTRK inhibitor therapy.
PTEN (phosphatase and tensin homolog) is a tumor suppressor that negatively regulates the PI3KAKT signaling pathway. Loss of PTEN function has been implicated in the pathogenesis of a number of different tumors, particularly endometrial cancer.
CD1a is part of a heterodimer with β-2-microglobulin, and mediates the capture and presentation of antigens, primarily lipid and glycolipid antigens of self or microbial origin, to T-cells. CD1a is expressed on interdigitating and dermal dendritic cells, veiled cells, Langerhans cells, antigen-presenting cells of the lymph nodes, and cortical thymocytes. Anti-CD1a stains Langerhans cell histiocytosis and cortical T LBL/L pre-T lymphoblastic lymphoma and leukemia. In concert with S100 and CD68, CD1a is very useful for differentiating Rosai-Dorfman disease from other histiocytic diseases.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC530
Antibody Isotype:
IgG1
GMDN Code:
56922
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Skin, Thymus
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Cluster of Differentiation (CD20), also known as B-Lymphocyte Antigen, is a non-glycosylated protein expressed on the surface of normal and malignant B-cells, which functions in chemokine signaling and microenvironmental interactions of B-cells. Anti-CD20 stains a minority of Reed-Sternberg cells with Hodgkin's disease. Since CD20 does not stain T-cell malignancies, it is a very useful marker for B-cell lymphomas. CD20 is also not reactive on non-hematopoietic neoplasms.
Neuron-Specific Enolase (NSE), also known as Enolase 2 (ENO2), is one of three enolase enzymes found in mammals, and acts as a phosphopyruvate hydratase. This mammalian glycolytic isoenzyme is located specifically in neurons of neuroendocrine cells, as well as tumours associated with those neurons. However, it has also been detected immunohistochemically in non-neoplastic cells of the pituitary, peptide-secreting tissues, pinealocytes, neuroendocrine cells of the lung, thyroid, parafollicular cells, adrenal medulla, islets of Langerhans, Merkel cells of the skin, and melanocytes. NSE is a useful marker for identifying normal striated muscle, hepatocytes, and peripheral nerves. Anti-NSE may detect for neuroendocrine differentiation, only when used in a panel of antibodies including more specific markers such as synaptophysin, chromogranin, and neurofilament.
Neurofilaments are a group of intermediate filaments found abundantly around the axons of vertebrate neurons. They are also expressed in paragangliomas, adrenal pheochromocytomas, Merkel cell tumours, carcinoid tumours, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung. Anti-Neurofilament stains a variety of neural, neuroendocrine, and endocrine tumours, such as neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas.
ROS1 serves as a receptor tyrosine kinase. Gene rearrangement events involving ROS1 have been described in lung and other cancers, and such tumors have been found to be remarkably responsive to small molecule tyrosine kinase inhibitors. Multiple studies have demonstrated an incidence of approximately 1% in lung cancers, demonstrated oncogenicity, and showed that inhibition of tumor cells bearing ROS1 gene fusions by crizotinib or other ROS1 tyrosine kinase inhibitors was effective in vitro.
Cluster of Differentiation 21 (CD21) is a glycoprotein on the surface of B-cells that is bound by Epstein-Barr virus (EBV) during infection of these cells. CD21 staining is useful for recognizing follicular dendritic cell matrices in normal tonsillar and lymph tissue, and can also stain dendritic cell sarcomas. CD21 is also useful for distinguishing marginal zone lymphoma with follicular involvement from follicular lymphoma with marginal zone differentiation. When used in concert with other B- and T-cell markers, CD21 is valuable for differentiating between nodular lymphocyte-predominant Hodgkin's lymphoma, T-cell/histiocyte-rich B-cell lymphoma, and lymphocyte-rich classic Hodgkin's lymphoma. CD21 staining is useful for recognizing abnormal follicular dendritic cell patterns in angioimmunoblastic T-cell lymphoma and follicular T-cell lymphoma.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC533
Antibody Isotype:
IgG2a
GMDN Code:
56956
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Tonsil, Lymph Node
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
The TFE3 [IHC108] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissues within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
The Nestin [IHC105] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissues within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Applications:
IHC
Clone number:
IHC105
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Tonsil, Glioma
Buffer:
Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
S-100 is a low-molecular weight protein found in Schwann cells, melanocytes, glial cells, histiocytes, lipocytes, skeletal and cardiac muscle, chondrocytes, adipocytes, myoepithelial cells, macrophages, Langerhans cells, dendritic cells, and keratinocytes. S-100 is a useful marker for Schwann cell-derived tumours and a number of well-differentiated tumours of the salivary gland, adipose and cartilaginous tissue. Anti-S-100 is used to detect melanomas, histiocytosis X, malignant peripheral nerve sheath tumours, and clear cell sarcomas.
Nerve Growth Factor Receptor (NGFR), also known as p75, P-75NTR, or CD271, is a neurotrophin receptor belonging to the tumour necrosis factor receptor family. It is expressed mainly in Schwann cells and neurons, as well as a number of other non-neuronal cell types, and is also expressed in melanocytes, melanomas, neuroblastomas, pheochromocytomas, neurofibromas, neurotized nevi (type C melanocytes), and other neural crest cell or tumour derivatives. It has been suggested that NGFR may act as a tumour suppressor indicated in prostate and urothelial cancer, and Anti-NGFR is often used in adjunct with S100, to aid in the diagnosis of desmoplastic and neurotrophic malignant melanomas. Anti-NGFR is also useful as an aid in the diagnosis of breast malignancy, as the antibody labels the myoepithelial cells of breast ducts and intralobular fibroblasts of breast ducts.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC637
Antibody Isotype:
IgG1, kappa
GMDN Code:
57471
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Breast
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
The Factor XIIIa [IHC572] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissue within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Applications:
IHC
Clone number:
IHC572
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Placenta
Buffer:
Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
Cluster of Differentiation 23 (CD23) is found on interleukin-4 activated B-cells, activated macrophages, eosinophils, and follicular dendritic cells, and is a receptor for IgE, an antibody involved in parasitic immunity. CD23 is present on Reed-Sternberg cells in Hodgkin's disease. Follicular dendritic cells and activated B-lymphocytes produce strong staining in germinal centers and weak patterns in mantle zone B-cells. CD23 is helpful in differentiating chronic lymphocytic leukemia from mantle cell leukemia. Small B-cell lymphomas are sometimes positive, while precursor B- and T-lymphomas, myeloid neoplasms, and mature T-cell lymphomas stain negatively with Anti-CD23.
Cluster of Differentiation 3 (CD3) is a T-cell co-receptor expressed by T-cells in thymus, peripheral lymphoid tissue, blood, and bone marrow, as well as activated natural killer cells. CD3 is specifically expressed by T-cells at all stages of development including T-cell lymphomas and leukemias; therefore, it can be used to classify T-cell neoplasms from B-cell and myeloid neoplasms.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC534
Antibody Isotype:
IgG
GMDN Code:
56926
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Tonsil
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
N-cadherin, also known as Cadherin-2 (CDH2) or Neural Cadherin (NCAD), is a transmembrane cell adhesion molecule that was originally detected in nervous tissue. It plays an important role in embryogenesis, being involved in gastrulation and neural crest development. N-cadherin is found in cancer cells and allows for transendothelial migration, which is a critical process in the metastasis of cancer. Overexpression and disorderly arrangement of N-cadherin has been noted in dilated cardiomyopathy. It has been suggested that, when considered in adjunct with the status of a number of additional cell-cell adhesion molecules, missense mutations in N-cadherin may be a potential indicator of obsessive-compulsive disorder and Tourette disorder.
Cytokeratin 8 & 18 are present in various epithelia including that of the breast, thyroid, respiratory tract, and gastrointestinal tract. Anti-Cytokeratin 8 & 18 stains adenocarcinomas and most non-keratinizing squamous carcinomas, but does not stain keratinizing squamous carcinomas. Since Cytokeratin 18 is scarce in normal epidermis, Anti-Cytokeratin 8 & 18 is used to detect Paget cells in such samples. Cytokeratin 8 & 18 helps identify colorectal carcinoma metastases as it is more sensitive than genetic tests.
Napsin A is a pepsin-like aspartic proteinase that is closely related to Napsin B. It is expressed mainly in the lung and kidney, and is involved in the correct folding, targeting, and control of aspartic proteinase zymogens. Napsin A expression has been indicated in type II pneumocytes and adenocarcinomas of the lung and kidney. Anti-Napsin A is also useful for differentiating between primary lung adenocarcinomas and adenocarcinomas of other organs, due to the high expression of Napsin A in adenocarcinomas of the lung.
Serum Amyloid A1 (SAA1) is a member of the serum amyloid A family of apolipoproteins. Highly inducible SAA1 is an acute phase protein expressed in response to tissue injury and chronic inflammatory disease. The recent studies also suggest SAA1 is associated with tumor pathogenesis contributing to certain types of malignant tumors.
Nanog is a homeoprotein that functions with pluripotent factors, such as Oct-4 and SOX2, to maintain embryonic stem cell pluripotency. Expression of this protein has been noted in seminoma, dysgerminoma, embryonal carcinoma, and other undifferentiated germ cell tumours, while Nanog expression is absent in normal adult organ tissues. Anti-Nanog may be useful in distinguishing between undifferentiated germ cell tumours and non-germ cell tumours.
Enhancer of Zeste Homolog 2 (EZH2) is a methylase of histone H3 that silences gene expression in those regions. EZH2 is overexpressed or mutated in gastric, prostate, uterine, breast, and renal cell cancers, as well as in melanoma and most B- and T-cell lymphomas. Although EZH2 is usually present in follicular centers, it is not expressed in the mantle zones, plasma cells, follicular or interfollicular T-lymphocytes, natural killer T-lymphocytes, plasmacytoma, lymphoplasmacytic lymphoma, or MALT lymphoma. EZH2 is rarely present in normal breast duct epithelium and in normal and hyperplastic lymph node. Anti-EZH2 is also useful for detecting lymphoma and non-small cell lung cancers. EZH2 is associated with tumour proliferation and can be used in staining panels to distinguish aggressive lymphomas from less aggressive lymphomas or normal cells.
Cluster of Differentiation 30 (CD30) is a transmembrane cytokine receptor expressed by activated T- and B- cells. It is present on Reed-Sternberg cells in Hodgkin's lymphoma, most anaplastic large cell lymphomas, embryonal carcinomas, and primary cutaneous CD30 positive T-cell lymphoproliferative disorders. B-cell lymphomas are sometimes stained by Anti-CD30. Lymphomas exhibit Golgi zone accentuation when stained with Anti-CD30, while embryonal carcinomas produce membranous stains.
Smooth Muscle-Myosin is a major component of smooth muscle contractile apparatus. IHC detection of Myosin, Smooth Muscle visulizes the myoepithelial cell present in both normal and in situ malignant breast and bronchioloalveolar lesions and serves a valuable biomarker to distinguish between benign and malignant tumors.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC091
GMDN Code:
57591
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Breast, Colon
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Thyroid-Stimulating Hormone (TSH) is secreted by thyrotrope cells in the anterior pituitary gland. Anti-TSH stains thyrotrophs and is useful for categorizing pituitary tumours, as well as for recognizing primary and metastatic pituitary gland tumours.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC679
Antibody Isotype:
IgG1
GMDN Code:
57663
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Pituitary
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Cluster of Differentiation 31 (CD31) is present on hematopoietic stem cells (HSCs), and its expression is used to determine the concentration of HSCs in research studies and for bone marrow transplantation. Anti-CD31 is very specific and sensitive for endothelial cells and does not stain non-vascular tumours, therefore CD31 staining is used to recognize the vascular origins of neoplasms.
Sal-Like Protein 4 (SALL4) is a zinc finger transcription factor found in germ cells and human blood progenitor cells, with functional involvement in modulating Oct-4 to maintain embryonic stem cell pluripotency. SALL4 is a useful marker for acute myeloid leukemia, B-cell acute lymphocytic leukemia, intratubular germ cell neoplasia, seminomas/dysgerminomas, and yolk sac tumours (both pediatric and postpubertal). Anti-SALL4 is used to detect embryonal carcinomas, hepatocellular carcinoma (HCC), gliomas, ovarian primitive germ-cell tumours, choriocarcinomas, spermatogonia, teratoma, gastric cancer, breast cancer, and lung cancer. Expression of SALL4 is often associated with poor prognosis in HCC, and with metastasis in endometrial cancer, colorectal carcinoma, and esophageal squamous cell carcinoma.
CD317, also known as BST2, tetherin, HM1.2 antigen, DAMP-2, is an integral transmembrane glycoprotein which may play a role in pre-B-cell growth, rheumatoid arthritis, and in antiretroviral defense, that blocks release of retrovirus from the cell surface. It is highly expressed on terminally differentiated normal plasmacytoid dendritic cells and some tumor cells, such as multiple myeloma, renal cell carcinoma, and melanoma cells.
Cluster of Differentiation 34 (CD34) is a transmembrane glycoprotein expressed on hematopoietic stem and progenitor cells, vascular endothelium, embryonic fibroblasts, and rare glial cells in nervous tissue. CD34 is the most used marker for characterizing blasts in leukemia. CD34 is also present in some soft tissue tumours including solitary fibrous tumours and gastrointestinal stromal tumours. Proliferating endothelial cells seem to upregulate CD34 expression. Although specificity is low, Anti-CD34 reacts positively with more than 85% of angiosarcoma and Kaposi’s sarcoma.
Myogenin belongs to a family of myogenic transcription factors, including MyoD, Myf5, and MRF4, which are critical in muscle development. Myogenin is found strictly in cells of skeletal muscle origin, and is therefore used as a biomarker for tumours of the muscle lineage, including alveolar rhabdomyosarcomas. Anti-Myogenin staining may occur in Wilms' tumour, and it labels the nuclei of myoblasts in developing muscle tissue. It is also expressed in some leiomyosarcomas.
Estrogen Receptors (ER) are a group of nuclear hormone receptors activated by the hormone estrogen. ER is found in normal epithelial cells of the breast and endometrium, as well as in breast cancer cells.
Cluster of Differentiation 35 (CD35), also known as Erythrocyte Complement Receptor 1 (CR1) or C3b/C4b, is commonly found on erythrocytes, B- and T-cells, monocytes, eosinophils, and neutrophils. It functions to mediate the clearance of opsonized targets. CD35 is a mature B-lymphocyte marker, and Anti-CD35 reacts positively with normal and tumourous follicular dendritic reticulum cells.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC035
Antibody Isotype:
IgG1
GMDN Code:
56976
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Tonsil, Placenta
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
The TOP2A [IHC112] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of proliferating cells in normal and neoplastic tissues within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Applications:
IHC
Clone number:
IHC112
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Tonsil, Cervix
Buffer:
Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
The SATB2 [IHC095] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissues within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Applications:
IHC
Clone number:
IHC095
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Colon Adenocarcinoma
Buffer:
Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
Multiple Myeloma Oncogene-1 (MUM1), also known as Interferon Regulatory Factor 4 (IRF4), is a transcription factor present in a variety of hematolymphoid neoplasms and in malignant melanoma, but is absent from other human tumours. MUM1 expression has been indicated in both pediatric and adult diffuse large B-cell lymphoma (DLBCL), and, when the immunostaining status of CD10 and Bcl6 is also considered, Anti-MUM1 can be used to sub-distinguish germinal center type DLBCL from the non-germinal center type. Anti-MUM1 stains normal melanocytes, melanocytic nevi, and malignant melanoma in non-hematopoietic tissues, and can also stain other B-cell lymphomas such as lymphoplasmacytic lymphoma, grade 3 follicular lymphoma, primary central nervous system lymphoma, primary mediastinal large B-cell lymphoma, Burkitt-like lymphoma, and classic Hodgkin's lymphoma.
Mucin 6 (MUC6) is a glycoprotein expressed in mucous neck cells, pyloric glands of the antrum, epigastric and bronchial epithelium, and in Müller ducts of the endocervix and urethral epithelium. Anti-MUC6 is useful for differentiating fetal, precancerous, and cancerous colonic mucosa from normal colon, as the antibody does not stain the latter. Anti-MUC6 stains the gastric epithelial surface of normal human gastrointestinal tracts.
Cluster of Differentiation 4 (CD4) is a membrane glycoprotein expressed in T helper cells, monocytes, macrophages, granulocytes, and dendritic cells, and is a receptor of human immunodeficiency virus (HIV). CD4 staining is used for identifying lymphoproliferative disorders. Since the majority of peripheral T-cell lymphomas arise from the T helper cell subset, CD4 expression can be found in most forms of T-cell lymphomas as well as anaplastic large T-cell lymphomas and mycosis fungoides. Since CD4 may be aberrantly expressed in neoplastic T-lymphocytes, a panel of markers may be used to identify such tumours. CD4(+) CD25(+) T-cells are reported to exert immunosuppression, which is commonly observed in various types of cancers, including non-small cell lung cancer and cancers of the breast, prostate, and ovary.
Mucin 5AC (MUC5AC) is a secretory-type mucin found in columnar mucous cells of surface gastric epithelium and in goblet cells of the fetal and precancerous colon, but not in normal colon cells. MUC5AC expression is indicated in carcinomas wherein the type is defined as diffuse and infiltrative, and those located mainly in the antrum. Studies have also suggested a correlation between MUC5AC and colorectal signet ring cell carcinoma, with overexpression of MUC5AC relating to the carcinogenesis, malignant potential, progression, and clinical behaviors.
Cluster of Differentiation 44 (CD44) is a glycoprotein receptor for hyaluronic acid that plays a fundamental role in cellular adhesion, stromal binding, migration, and cell-cell interactions. Positive staining with Anti-CD44 is implicated in a multitude of different cancer types, including breast, prostatic, renal cell, colonic, hepatocellular, and genitourinary carcinomas, as well as non-Hodgkin's Lymphoma, metastatic melanoma, gastric cancer, and some soft tissue tumours. It has also been demonstrated that there is a positive correlation between tumour progression and increased expression of CD44v, a high molecular weight CD44 isoform that has been described in epithelial cells. Given the expression of CD44 in a wide range of cancers, the most practical application of CD44 immunostaining is its use in discriminating between urothelial transitional cell carcinoma <em>in situ</em> from non-neoplastic changes in the urothelium.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC043
Antibody Isotype:
IgG
GMDN Code:
56984
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Benign Urothelium
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
The NRP1 [IHC121] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissues within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Applications:
IHC
Clone number:
IHC122
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Brain
Buffer:
Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
Erythroblastosis Virus E26 Transforming Sequence Related Gene (ERG) facilitates endothelial homeostasis. ERG is found in malignant and benign vascular endothelial tumours, including hemangiomas and Kaposi's sarcoma. ERG is present in various prostate carcinomas, but is absent in breast, colon, and urothelium carcinomas. Anti-ERG is useful for differentiating prostate carcinoma from non-prostatic epithelial tumours, and for recognizing vascular endothelial neoplasms.
Mucin 1 (MUC1) is a membrane-bound glycoprotein involved in a number of protective and cell-signaling functions, including cell-cell adhesion, proliferation, motility, invasion, and survival. Overexpression of MUC1 is clinically indicated in breast carcinomas, papillary thyroid carcinomas, and thymic carcinomas, and reports have named MUC1 as a useful marker for differentiating thymic carcinoma from type B3 thymoma. The expression of MUC1 is correlated with the grade of malignancy in thymic epithelial tumours, and loss of MUC1 expression has been associated with reactive gastropathy. MUC1 is not expressed in normal human epidermis, but it has been detected in the epidermis of psoriatic plaques of biopsies from patients diagnosed with psoriasis vulgaris.
Transforming Growth Factor ?1 (TGF?1) is a cytokine present in regulatory T-cells, immature dendritic cells, megakaryocytes, and platelets, with a functional involvement in regulating T-cells. TGF?1 is overexpressed in thyroid cancer and cervical squamous cell carcinoma. Poor prognosis of cervical squamous cell carcinoma is associated with TGF?1.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC673
GMDN Code:
?
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Placenta
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Cluster of Differentiation 44 (CD44) is a glycoprotein receptor for hyaluronic acid that plays a fundamental role in cellular adhesion, stromal binding, migration, and cell-cell interactions. Positive staining with Anti-CD44 is implicated in a multitude of different cancer types, including breast, prostatic, renal cell, colonic, hepatocellular, and genitourinary carcinomas, as well as non-Hodgkin's Lymphoma, metastatic melanoma, gastric cancer, and some soft tissue tumours. It has also been demonstrated that there is a positive correlation between tumour progression and increased expression of CD44v, a high molecular weight CD44 isoform that has been described in epithelial cells. Given the expression of CD44 in a wide range of cancers, the most practical application of CD44 immunostaining is its use in discriminating between urothelial transitional cell carcinoma <em>in situ</em> from non-neoplastic changes in the urothelium.
MutS Homolog 6 (MSH6) is a protein involved in the mismatch repair pathway. This protein is commonly associated with hereditary non-polyposis colorectal cancer, and mutations in this gene are correlated with the development of sporadic colorectal carcinoma. Studies have shown that mutations in MSH6, when co-indicated with mutations in MSH1 and MSH2, contribute to the development of sporadic colorectal carcinoma. Use of Anti-MSH2 is optimized when paired with MSH6, MLH1, and PMS2 in an IHC panel.
Cluster of Differentiation 45 (CD45), also known as Leukocyte Common Antigen (LCA), is a member of the protein tyrosine phosphatase (PTPase) family that is known to regulate a variety of cellular processes including cell growth, differentiation, the mitotic cycle, and oncogenic transformation. CD45 is expressed in most nucleated cells of hematopoietic origin, and is an essential regulator of T- and B-cell antigen receptor signaling. Anti-CD45 positively stains the majority of lymphoid neoplasms, and is highly indicative of lymphoid origin. However, an absence of CD45 does not rule out lymphoid tumours, as certain types of neoplasms lack CD45, such as Hodgkin's lymphoma, some T-cell lymphomas, and some leukemias.
MutS Homolog 2 (MSH2) is a protein involved in the mismatch-repair pathway. This protein is commonly associated with hereditary non-polyposis colorectal cancer, and mutations in this gene are correlated with the development of sporadic colorectal carcinoma. Expression levels of MSH2 are abnormally low in a high percentage of patients with microsatellite instability, as well as endometrial and ovarian cancers. Use of Anti-MSH2 is optimized when paired in an IHC panel with antibodies against MSH6, MLH1, and PMS2. Reports have shown Anti-MSH2 to be useful in the detection of the protein in a number of normal and neoplastic tissues, and for identifying a loss of MSH2 in tumours that are microsatellite-unstable.
Excision Repair Cross Complementing 1 (ERCC1) is a DNA repair enzyme involved in the repair of UV-induced DNA damage. ERCC1 overexpression is associated with tumour progression in many malignancies, such as ovarian cancer, head squamous cell carcinoma, non-small cell lung cancer (NSCLC), and esophageal cancer.
CD45R, also known as MB1, is an isoform of CD45 that is a member of the protein tyrosine phosphatase (PTPase) family. CD45R is expressed specifically on the surface of hematopoietic cells, and has demonstrated function as a regulator of the antigen and cytokine receptor signaling of B- and T-cells. Given that the antigen is located in the membrane of all B-cells, with the exception of plasma cells and some mature T-cells, Anti-CD45R exhibits specific reactivity with most B-lymphocytes. The use of Anti-CD45R is primarily useful in distinguishing B-cell lymphomas from T-cell lymphomas, with specific reactivity to follicle center cells, mantle cells, some medullary thymocytes, and 80% of B-cell lymphomas.
Villin is a tissue-specific actin-binding glycoprotein that is associated with the maintenance of the microvilli brush border found in the gastrointestinal mucosal epithelium. Villin is expressed in carcinomas of the gastrointestinal tract, renal cell carcinomas, pacreatic carcinomas, endometrial carcinomas, as well as carcinomas of the ovary and lungs. Anti-Villin antibodies can be useful for identifying and differentiating adenocarcinomas of these organs from other organs in the body. Additionally, it may be helpful in separating carcinoid tumors from other endocrine tumors.
MutL Homolog 1 (MLH1) is a protein involved in the mismatch-repair pathway. This protein is commonly associated with hereditary non-polyposis colorectal cancer, as the MLH1 gene is frequently mutated in patients with this cancer. Studies have shown MLH1 to be deficient in a high percentage of patients with microsatellite instability, as well as endometrial and ovarian cancers. Use of Anti-MLH1 is optimized when paired in an IHC panel with MSH6, MSH2, and PMS2. Anti-MLH1 is useful in the detection of MLH1 in a number of normal and neoplastic tissues, and for identifying a loss of MLH1 in tumours that are microsatellite-unstable.
CD45RO is an isoform of CD45 which is expressed in thymocytes, activated T-cells, and subpopulations of resting T-cells. It is a useful marker for T-cell tumours, as Anti-CD45RO demonstrates no reactivity with B-cells. Specifically, CD45RO is implicated in a number of T-cell lymphomas including angioimmunoblastic, lymphoblastic, peripheral, and subcutaneous panniculitis-like T-cell lymphomas.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC537
Antibody Isotype:
IgG2a
GMDN Code:
56992
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Tonsil
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Multidrug Resistance 3 (MDR3), also known as ATP Binding Cassette Subfamily B Member 4 (ABCB4), is a membrane-associated protein belonging to the superfamily of ATP-binding cassette transporters. MDR3 is an energy-dependent phospholipid efflux translocator that mediates the translocation of phosphatidylcholine across the canalicular membrane of the hepatocyte, and also acts as a positive regulator of biliary lipid secretion. Defects in MDR3 are associated with progressive familial intrahepatic cholestasis type 3 and gallbladder disease type 1. Co-overexpression of MDR3 and MRP1 has been documented as correlating with blastemal subtype and high-risk prognosis of Wilms' tumour patients.
SRY (Sex Determining Region Y)-Box 10 (SOX-10), also known as Transcription Factor SOX-10, is a nuclear transcription factor that acts in regulation of embryonic development and in the specification and differentiation of cells of melanocytic lineage. SOX-10 is diffusely expressed in neurofibromas and schwannomas, and mutations in the SOX-10 gene are linked to Waardenburg-Shah and Waardenburg-Hirschsprung's disease. Anti-SOX-10 has been shown to be sensitive for conventional, spindled, and desmoplastic melanoma, and has been used to detect metastatic melanoma and nodal capsular nevus in sentinel lymph nodes.
Cluster of Differentiation 5 (CD5) is expressed in high levels on the surface of T-cells, while the expression levels and role of CD5 in B-cells is not well documented. As a part of a diagnostic panel, its utility lies predominantly as a marker for T-cells, with over 70% of T-cell neoplasms expressing CD5. In particular, it is correlated with chronic lymphocytic leukemia and small lymphocytic lymphomas, mantle cell lymphoma, as well as a subset of diffuse large B-cell lymphomas. CD5 demonstrates positive expression in thymic carcinomas, and is not as sensitive as CD3. CD5 also has value as a prognostic indicator, as it is associated with poor prognosis in acute T-cell lymphoblastic leukemia.
Mouse Double Minute 2 Homolog (MDM2), also known as HDM2 in humans, is crucial in negative regulation of the p53 tumour suppressor. Negative regulation is mediated through both the ubiquitination of p53/TP53, as well as inhibition of p53 transcriptional activation. Reports have indicated an overexpression of MDM2 to be associated with a number of different human tumour types, including soft tissue sarcomas, osteosarcomas, and breast tumours. When co-overexpressed with the CDK4 protein, MDM2 can also aid in the detection of well-differentiated liposarcomas and de-differentiated liposarcoma.
SRY (Sex Determining Region Y)-Box 11 (SOX11), also known as Transcription Factor SOX11, is a nuclear transcription factor that acts in regulation of embryonic development, cell differentiation, and the development of the human central nervous system. SOX11 is expressed in medulloblastoma and glioma, and has been indicated as a marker for both Cyclin D1-positive and -negative mantle cell lymphomas, Burkitt's lymphoma, and lymphoblastic lymphoma.
CDX-2 is a caudal-related homeobox transcription factor that is expressed by intestinal epithelial cells. CDX-2 is a useful marker for gastrointestinal carcinoma and for determining the origin of gastrointestinal metastatic adenocarcinoma and carcinoid tumours. Anti-CDX-2 is used for differentiating lung and metastatic colorectal adenocarcinoma. However, mucinous ovarian carcinoma also reacts positively with Anti-CDX-2, thereby limiting the ability to differentiate from metastatic colorectal adenocarcinoma.
SOX-2, also known as SRY (Sex Determining Region Y)-Box 2, is a transcription factor that acts to regulate pluripotency of undifferentiated embryonic stem cells, and to regulate gene expression in the stomach. This diagnostic grade SOX-2 IVD antibody is used to detect melanoma, testicular germ cell tumour, cervical carcinoma, lung cancer, breast cancer with basal cell phenotype, and teratoma of the central nervous system. SOX-2 has been reported as a predictor of poor outcome in stage I lung adenocarcinomas. Anti-SOX-2 is also used to recognize squamous cell carcinomas of the lung and gastrointestinal tract, and may be useful for detecting embryonal carcinoma.
Carcinoembryonic Antigen (CEA) describes a set of glycophosphatidyl inositol and transmembrane cell-surface-anchored glycoproteins involved in cell adhesion, differentiation, anoikis, polarization, and tissue architecture. CEA staining, along with Calretinin, CK 5/6, D2-40, HBME-1, Napsin A, MOC-31, and Ber-EP4, is used to help differentiate between adenocarcinoma and mesothelioma. Staining with Anti-CEA is also suggested to be useful in identifying the origin of metastatic adenocarcinoma. CEA is an effective marker for adenocarcinomas of the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix.
MART-1, also known as Melan A or Melanoma Antigen Recognized by T-Cells 1, is a protein antigen found specifically on melanocytes of normal skin, retina, and nevi, and not in other normal tissues. Anti-MART-1 is therefore useful as a marker for melanocytic tumours, and as an aid in establishing the diagnosis of metastatic melanomas.
SPNS2 is a sphingolipid transporter expressed in the extraembryonic yolk syncytial layer (YSL). SPNS2 is required of migration of myocardial precursors, since it contributes in sphingosine 1-phosphate (S1P) secretion, which is a mediator that plays critical roles in cardiovascular, immunological, and neural development and function.
Chromogranin A is localized in secretory granules of neurons and endocrine cells in tissues, including pituitary, adrenal medulla, thyroid, pancreatic islets, and the gastrointestinal tract. Neuroendocrine cells exhibit a fine granular immunoreactivity to Anti-Chromogranin A. It is widely recognized that co-expression of keratins and chromogranin A implies a neuroendocrine lineage. High expression of chromogranin A and negative staining with Anti-Keratin is a possible indication of paraganglioma. Positive staining for chromogranin A and neuron-specific enolase is representative of neuroendocrine neoplasms. Many pituitary adenomas and prolactinomas stain positively for chromogranin A.
MART-1, also known as Melan A or Melanoma Antigen Recognized by T-Cells 1, is a protein antigen found specifically on melanocytes of normal skin, retina, and nevi, and not in other normal tissues. Anti-MART-1 is therefore useful as a marker for melanocytic tumours, and as an aid in establishing the diagnosis of metastatic melanomas.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC408
Antibody Isotype:
IgG1
GMDN Code:
57393
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Melanoma, Skin
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Epithelial Cell Adhesion Molecule (EpCAM) is a transmembrane glycoprotein that mediates cell-cell adhesion in epithelia. It is normally present on most baso-lateral surfaces of normal epithelial cells and is absent in myoepithelial cells, hepatocytes, adult squamous epithelia, mesothelial cells, and fibroblasts. Anti-EpCAM stains most adenocarcinomas and neuroendocrine tumours, including small cell carcinomas. A minority of renal clear cell carcinoma, renal oncocytoma, and hepatocellular carcinoma stain positively for EpCAM, while Anti-EpCAM stains nearly all basal cell carcinoma. Anti-EpCAM stains chromophobe renal cell carcinoma, papillary renal cell carcinoma, and cholangiocarcinoma more frequently. Anti-EpCAM can be useful for distinguishing malignancy in the peritoneal and pleural cavities.
The Chromogranin B [IHC497] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissue within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Applications:
IHC
Clone number:
IHC497
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Pancreas
Buffer:
Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
T-Cell Immunoglobulin and Mucin-Domain-Containing Molecule-3 (TIM3) is present on T-helper type 1 lymphocytes and other immune cells, including dendritic cells and natural killer cells. TIM3 is overexpressed in CD4+ tumour-infiltrating lymphocytes, including those with non-small cell lung cancer associated with poor prognoses. TIM3 has recently emerged as a potential target for cancer immunotherapy.
Clusterin/Apolipoprotein J is normally found in epithelial cells, semen, plasma, breast milk, cerebrospinal fluid, and urine, and is involved in apoptosis and the clearance of cellular debris. It is present in hematopoietic and non-hematopoietic cancers, as well as most systemic anaplastic large cell lymphomas. Anti-Clusterin/Apolipoprotein J in a panel with other antibodies is useful for differentiating systemic anaplastic large cell lymphoma from classic Hodgkin's disease. Anti-Clusterin/Apolipoprotein J also displays high sensitivity and specificity for follicular dendritic cell tumours. Clusterin overexpression is linked to recurrence and poor prognosis in breast cancer, and chemosensistivity and poor survival in cervical cancer.
Luteinizing Hormone (LH) is a reproductive hormone produced and secreted by the gonadotropes in the anterior pituitary gland. LH functions to stimulate ovulation in females and the production of testosterone from the Leydig cells in males. This hormone is useful for the study of pituitary disease, and acts as a clinical marker that is useful for classifying tumours of the pituitary.
Epithelial Membrane Antigen (EMA) is a mucin glycoprotein expressed on apical epithelial cells. Anti-EMA positively stains normal and neoplastic cells including sweat glands, mammary epithelia, and squamous epithelia. Adrenal carcinoma, seminomas, paraganglioma, hepatocellular carcinoma, and embryonal carcinomas exhibit a negative stain. As Anti-EMA commonly reacts positively with meningioma, it is useful for differentiating this tumour from other intracranial neoplasms such as schwannomas.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Applications:
IHC
Clone number:
IHC085
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Breast, Skin
Buffer:
Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
The C-Met [IHC078] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissues within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
The Lp-PLA2 [IHC407] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of cardiovascular diseases within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Applications:
IHC
Clone number:
IHC407
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Tonsil, Thymus, Placenta
Buffer:
Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
Thymidylate Synthase (TS) is a crucial enzyme responsible for the synthesis of 2'-deoxythymidine-5'-monophosphate (dTMP) a precursor for thymidylate which is necessary for DNA replication and repair from 2'-deoxyuridine-5'-monophosphate (dUMP). In terms of cancer, TS is an important target for cancer treatment as the inhibition of TS and therefore nucleotide synthesis necessary for cell growth has shown to be a vital part for successful treatment against colorectal, pancreatic and breast cancers.
Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five types) and two light chains - kappa and lambda. The determination of light chain ratio is critical in evaluating B-cell neoplasms, as the majority of B-cell lymphomas express either kappa or lambda light chains, while a mixture of kappa and lambda is characteristic of reactive proliferations. Antibodies against lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas.
LAG3 (Lymphocyte-activation gene 3) was discovered in 1990 and was previously designated as CD223. LAG3 is a cell surface molecule expressed by activated T cells, natural killer cells, B cells and plasmacytoiddendritic cells. It binds to major histocompatibility complex (MHC) class II molecules and serves as an immune checkpoint receptor. LAG3 negatively regulates cellular proliferation, activation and homeostasisof T cells, and plays a role in Treg suppressive function. LAG3 also helps maintain CD8+ T cells in atolerogenic state and, working with PD-1, helps maintain CD8 exhaustion during chronic viral infection. LAG3 expression was detected in tumor infiltrating lymphocytes. IHC revealed LAG3 expression was distributed on lymphocytes scattered in renal cell carcinoma, melanoma and lymphomas. They were also detected in the tumor stroma as well as in the peritumoral tissue. LAG3 is the target of various drug development programs for cancer and autoimmune disorders. In soluble form, it is also being developed as a cancer drug in its own right.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC127
GMDN Code:
?
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Tonsil
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Epidermal Growth Factor Receptor (EGFR) is a tyrosine kinase present in gliocytes, epithelial cells, fibroblasts, keratinocytes, and other cell types. EGFR is overexpressed in various cancers including those of the colon, pancreas, oropharynx, stomach, and non–small cell lung, as well as head and neck squamous carcinoma and anal squamous carcinoma. EGFR expression is common in breast cancer, especially in triple-negative and basal-like breast carcinomas, and recent research has also found EGFR expressed in malignant bone and soft tissue cancers. Anti-EGFR is useful for detecting epithelioid and synovial sarcoma.
This gene, CMTM6, belongs to the chemokine-like factor superfamily, a novel family that is similar to the chemokine and transmembrane 4 superfamilies. CMTM6 stabilizes plasma membrane expression of PD-L1 and protects PD-L1 from lysosomal degradation by preventing STUB1-mediated PD-L1 ubiquitination. Based on the studies of CMTM6 immune system regulation, it is being investigated as an immunotherapeutic target for cancer treatment.
Ki-67 is a nuclear, non-histone protein that is expressed only during active phases of the cell cycle (G1, S, G2 and M), but not in the resting phases (G0 and G1 early phase). Although the antigen has also been associated with ribosomal RNA transcription, it is strongly linked to cell proliferation and has thus been indicated as an effective marker in grading the proliferation rate of tumours, including those of the brain, breast, cervix, and prostate.
Thyroglobulin is a precursor to the thyroid hormones T4 and T3, and is present in the thyroid follicular cells. Nearly all thyroid follicular carcinomas stain for thyroglobulin and sometimes produce a focal staining pattern. Conversely, poorly differentiated carcinomas and non-thyroid adenocarcinomas do not stain for thyroglobulin, therefore Anti-Thyroglobulin is a useful diagnostic tool for recognizing papillary and follicular thyroid carcinomas. A panel of Anti-Thyroglobulin and Anti-Calcitonin is useful for identifying medullary thyroid carcinomas, whereas a panel of Anti-Thyroglobulin and Anti-TTF1 is useful for distinguishing between primary thyroid and lung neoplasms.
Ki-67 is a nuclear, non-histone protein that is expressed only during active phases of the cell cycle (G1, S, G2 and M), but not in the resting phases (G0 and G1 early phase). Although the antigen has also been associated with ribosomal RNA transcription, it is strongly linked to cell proliferation and has thus been indicated as an effective marker in grading the proliferation rate of tumours, including those of the brain, breast, cervix, and prostate.
STAT6 is a member of the signal transducers and activators of transcription (STAT) family. Recurrent fusions of STAT6 with NAB2 on chromosome 12q13 have been found in the majority of solitary fibrous tumors (SFT). STAT6 antibody is a reliable immunohistochemical marker for SFT and can be helpful to distinguish this tumor type from histologic mimics.
c-Myc is a phosphoprotein involved with cell proliferation and differentiation. It is a useful marker for differentiation between Burkitt's lymphoma (BL) and diffuse large B-cell lymphoma (DLBCL) since, despite morphological similarities between the two B-cell lymphomas, Anti-c-Myc stains all BL and only a few DLBCL cases. A panel of antibodies against c-Myc, CD10, BCL2, and Ki-67 is useful for cases where Myc FISH analysis is warranted or can be omitted. Nuclear c-Myc overexpression is common in luminal cells of prostate intraepithelial neoplasia, many primary carcinomas, and metastatic disease.
KBA.62, also known as Melanoma Associated Antigen, is used to detect an antigen present in melanocytic tumours, such as melanomas, due to its proven sensitivity and specificity. The antibody can also be used to distinguish between junctional nevus and intradermal nevus cells, and fetal melanocytes versus normal adult melanocytes. Studies have shown KBA.62 to be highly useful in differentiating between metastatic amelanotic melanoma and a number of poorly differentiated carcinomas, large cell lymphomas, sarcomas, and spindle cell carcinomas.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC062
Antibody Isotype:
IgG1
GMDN Code:
57371
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Melanoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Anti-Kappa recognizes surface immunoglobulin on normal and neoplastic B-cells, and has been indicated as a potential aid in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas, where the expression of a single light chain class is restricted. The determination of light chain ratio is critical in evaluating B-cell neoplasms, as the majority of B-cell lymphomas express either kappa or lambda light chains, while a mixture of kappa and lambda is characteristic of reactive proliferations. In paraffin-embedded tissue, Anti-Kappa displays strong staining of kappa-positive plasma cells, as well as cells that have absorbed exogenous immunoglobulins.
Anti-Kappa recognizes surface immunoglobulin on normal and neoplastic B-cells, and has been indicated as a potential aid in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas, where the expression of a single light chain class is restricted. The determination of light chain ratio is critical in evaluating B-cell neoplasms, as the majority of B-cell lymphomas express either kappa or lambda light chains, while a mixture of kappa and lambda is characteristic of reactive proliferations. In paraffin-embedded tissue, Anti-Kappa displays strong staining of kappa-positive plasma cells, as well as cells that have absorbed exogenous immunoglobulins.
E-cadherin is an intercellular adhesion molecule present in epithelial cells. Anti-E-cadherin stains glandular epithelium, as well as lung, gastrointestinal, and ovarian adenocarcinomas. A panel of antibodies against E-cadherin and p120 is also used to differentiate ductal (membranous staining) and lobular breast cancer (cytoplasmic staining). Anti-E-cadherin also stains some thyroid cancers.
Human Chorionic Gonadotropin (hCG) is a glycoprotein hormone produced by the trophoblastic cells of the placenta after conception. Anti-hCG is useful for identifying trophoblastic tumours, such as choriocarcinoma. hCG is also a marker for non-trophoblastic tumours such as large cell carcinoma and lung adenocarcinoma.
Vascular Endothelial Growth Factor (VEGF) promotes vasculogenesis and angiogenesis, and mainly affects the vascular endothelium. VEGF is associated with poor prognoses of breast carcinomas, and has been shown to be elevated in rheumatoid arthritis.
Hepatitis B surface antigen (HBsAg) contains the large (L), middle (M), and small (S) surface proteins of the Hepatitis-B-Virus (HBV). It is the surface antigen of HBV, indicating current Hepatitis B infection. The body produces antibodies to HBsAg as part of the normal immune response to infection. Immunohistochemical staining for HBsAg in liver tissue is useful for the detection of HBV.
The PLAP [IHC088] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissues within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Applications:
IHC
Clone number:
IHC088
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Placenta
Buffer:
Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
Terminal Deoxynucleotidyl Transferase (TdT) is a DNA polymerase present in normal and malignant pre-B- and pre-T-cells during early differentiation. Anti-TdT stains nearly all acute lymphoblastic lymphoma/leukemia (ALL) cases, but does not stain pre-B-cell ALL or mature B- and T-cells. Anti-TdT staining is also useful for identifying Type AB thymoma and some chronic myeloid leukemia.
Perforin, a pore-forming protein found in the granules of cytotoxic T-lymphocytes and natural killer cells, functions to enable granzymes to enter the target cells and activate apoptosis. Perforin expression is upregulated in activated CD8+ T-cells, and these cells have been identified to have a major influence in Th1-associated inflammatory skin diseases. It has been suggested that perforin plays a role in alloimmunity, being involved in both the cytolytic process of rejection as well as downregulation of the T-cell mediated responses associated with the alloimmune response. Perforin-mediated cytotoxicity has also been linked to a number of autoimmune diseases.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC646
Antibody Isotype:
IgG1
GMDN Code:
63737
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Spleen
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Cytokeratin 5 dimerizes with Cytokeratin 14 to form the cytoskeleton of basal epithelial cells, while Cytokeratin 6 multimerizes with Cytokeratin 16 and/or 17 in the tongue, oral epithelia and esophagus, hair follicles, and glandular epithelia. Anti-Cytokeratin 5 & 6 rarely stains lung adenocarcinoma, but will produce small foci or scattered staining patterns in these Cytokeratin 5 & 6(+) samples. Cytokeratin 5 & 6 staining is useful for identifying squamous cell carcinoma, and can be used to determine the malignancies of myoepithelial cells in the breast and prostate. Cytokeratin 5 & 6 also rarely stains carcinomas of the breast, colon, and prostate. A panel of antibodies against Cytokeratin 5 & 6, TTF-1, napsin A, p63, SOX2, DSC3, and desmoglein-3 is useful for differentiating lung squamous cell carcinoma from lung adenocarcinoma and large cell carcinoma.
Programmed Death-Ligand 1 (PD-L1), CD274, or B7 Homolog 1 (B7-H1), is a transmembrane protein involved in suppressing the immune system and rendering tumour cells resistant to lysis through binding of the Programmed Death-1 (PD-1) receptor. Overexpression of PD-L1 may allow cancer cells to evade the actions of the host immune system. In renal cell carcinoma, upregulation of PD-L1 has been linked to increased tumour aggressiveness and risk of death. When considered in adjunct with CD8+ tumour-infiltrating lymphocyte density, expression levels of PD-L1 may be a useful predictor of multiple cancer types, including stage III non-small-cell lung cancer, hormone receptor negative breast cancer, and sentinel lymph node melanoma.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC441
Antibody Isotype:
IgG1, kappa
GMDN Code:
62046
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Tonsil, Lung Adenocarcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
FOXP3 is a forkhead transcription factor family member which plays a key role in CD4+CD25+ regulatory T cell function and represents a specific marker for these cells. Specifically in IHC, FOXP3 is a marker for adult T-cell leukemia/lymphoma (ATLL). In normal lymphoid tissues, a T-cell subset in interfollicular areas shows nuclear staining. There are many characteristics of FOXP3s role in cancer, which involves tumour progression through the suppression of T-cell activity and oncogene suppression through suppressing the expression of HER2, Skp2, SATB1 and MYC oncogenes.
Programmed Death-Ligand 1 (PD-L1), CD274, or B7 Homolog 1 (B7-H1), is a transmembrane protein involved in suppressing the immune system and rendering tumour cells resistant to lysis through binding of the Programmed Death-1 (PD-1) receptor. Overexpression of PD-L1 may allow cancer cells to evade the actions of the host immune system. In renal cell carcinoma, upregulation of PD-L1 has been linked to increased tumour aggressiveness and risk of death. When considered in adjunct with CD8+ tumour-infiltrating lymphocyte density, expression levels of PD-L1 may be a useful predictor of multiple cancer types, including stage III non-small-cell lung cancer, hormone receptor negative breast cancer, and sentinel lymph node melanoma.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC411
Antibody Isotype:
IgG
GMDN Code:
62046
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Tonsil, Lung Adenocarcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
The PD-L1-TEC [IHC451] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissue within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Applications:
IHC
Clone number:
IHC451
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Tonsil, Lung Adenocarcinoma
Buffer:
Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
Cytokeratin 7 (CK7) is a type II keratin which is present in transitional, ductal, glandular, and biliary duct epithelial cells. Cytokeratin 7 is a useful marker for distinguishing between carcinomas of the lung, breast, endometrium, and urothelia (positive stain) from carcinomas of the colon and prostate (negative stain). Cytokeratin 7 is present is nearly all primary lung adenocarcinomas, and is a useful marker in the differential diagnosis of ovarian neoplasms. Anti-Cytokeratin 7 does not stain intermediate filament.
The PD-L1-IMF [IHC461] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissue within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Applications:
IHC
Clone number:
IHC461
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Tonsil, Lung Adenocarcinoma
Buffer:
Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
Programmed Death 1 (PD-1) is a member of the CD28/CTLA-4 family of T-cell regulators, expressed as a co-receptor on the surface of activated T-cells, B-cells, and macrophages. New studies have suggested that the PD-1/PD-L1 signaling pathway may be linked to anti-tumour immunity, as PD-L1 has been shown to induce apoptosis of activated T-cells or inhibit activity of cytotoxic T-cells. In comparison to CD10 and Bcl-6, PD-1 is expressed by fewer B-cells and has therefore been considered a more specific and useful diagnostic marker for angioimmunoblastic T-cell lymphoma. Therapies targeted toward the PD-1 receptor have shown remarkable clinical responses in patients with various types of cancer, including non-small-cell lung cancer, melanoma, and renal-cell cancer.
Cytokeratin 8 (CK8) is present in single-layer epithelial tissue. CK8 frequently interacts with Cytokeratin 18, and Anti-Cytokeratin 8 is useful for identifying adenocarcinomas with simple epithelium origin. It may also be used to differentiate between lobular (perinuclear staining) and ductal (peripheral staining) breast carcinomas.
The PCNA [IHC711] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissues within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
Progesterone Receptor (PR), also known as NR3C3 (Nuclear Receptor Subfamily 3, Group C, Member 3), is an intracellular steroid receptor which mediates the physiological effects of progesterone, a female sex hormone involved in the menstrual cycle, pregnancy, and embryogenesis. Progesterone receptor expression has been linked to the prediction of prognosis in breast cancer, as well as associated responses to endocrine therapy. The progesterone receptor has also been linked to risk for ovarian cancer.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC751
Antibody Isotype:
IgG1, kappa
GMDN Code:
57534
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Breast, Breast Carcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Wilms' Tumour Protein (WT1) is a transcription factor involved in the development of the urogenital system. Anti-WT1 is utilized in the differential diagnosis of pulmonary malignancies (nuclei staining) and small round cell tumours. Ewing's sarcomas, primitive neuroectodermal tumours, neuroblastomas, rhabdomyosarcomas, and rhabdoid tumours do not stain with Anti-WT1, but cytoplasmic staining may be observed. Although lung adenocarcinomas do not exhibit nuclear staining with Anti-WT1, the antibody may stain the cytoplasm. Anti-WT1 also stains serous ovarian carcinomas, but does not stain mucinous carcinomas of the ovary and pancreatobiliary carcinomas.
PAX8 is expressed in simple ovarian inclusion cysts and non-ciliated mucosal cells of the fallopian tubes, but is absent from normal ovarian surface epithelial cells. Mutations in the PAX8 gene are linked to thyroid follicular carcinomas, atypical thyroid adenomas, and thyroid dysgenesis. Reports have associated PAX8 expression with renal carcinoma, nephroblastoma, and seminoma, and have indicated PAX8 as a useful marker for renal epithelial tumours, ovarian cancer, and for differential diagnoses in lung and neck tumours. Anti-PAX8 can be useful in determining the primary site of invasive micropapillary carcinomas of ovary from bladder, lung, and breast, when used in adjunct with a panel of organ-specific markers such as uroplakin, mammaglobin, and TTF-1.
Topoisomerase II alpha (TOP2A) is a member of a highly conserved group of enzymes that plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. The overexpression of TOP2A has been correlated with increased risk of progression in various cancers, and it has been a target for the development of anti-polymerase inhibitors to treat cancer.
PAX8 is expressed in simple ovarian inclusion cysts and non-ciliated mucosal cells of the fallopian tubes, but is absent from normal ovarian surface epithelial cells. Mutations in the PAX8 gene are linked to thyroid follicular carcinomas, atypical thyroid adenomas, and thyroid dysgenesis. Reports have associated PAX8 expression with renal carcinoma, nephroblastoma, and seminoma, and have indicated PAX8 as a useful marker for renal epithelial tumours, ovarian cancer, and for differential diagnoses in lung and neck tumours. Anti-PAX8 can be useful in determining the primary site of invasive micropapillary carcinomas of ovary from bladder, lung, and breast, when used in adjunct with a panel of organ-specific markers such as uroplakin, mammaglobin, and TTF-1.
Prolactin (PRL) is a peptide hormone synthesized and secreted by lactotroph cells in the adenohypophysis (anterior pituitary gland). PRL plays a role in a number of processes including cell growth, reproduction, and immune function, with its primary function being associated with lactation. Anti-Prolactin reacts with lactotroph cells, and is useful in classification of pituitary tumours and the study of pituitary disease.
PAX5 is a member of the paired box (PAX) family of transcription factors, which are key regulators in early development. The PAX5 gene encodes the B-cell lineage specific activator protein (BSAP), whose expression is limited to early stages of B-cell differentiation. Anti-PAX5 is useful in differentiating between classic Hodgkin's lymphoma versus multiple myeloma and solitary plasmacytoma, as the protein is expressed in mature and precursor B-cell non-Hodgkin's lymphomas/leukemias while being absent from the other two conditions. Diffuse large B-cell lymphomas are positive for PAX5, with the exception of those with terminal B-cell differentiation, and T-cell neoplasms do not stain with Anti-PAX5.
Prostate Cocktail is a combination of Cytokeratin 1, Cytokeratin 5, Cytokeratin 10, Cytokeratin 14, and p63. These four high molecular weight cytokeratins are found in basal epithelia of the prostate gland. p63 is a tumour suppressor protein found in basal epithelial nuclei of the normal prostate, while being negative in malignant tumours associated with the prostate gland. It is therefore useful in differentiating between benign and malignant prostate lesions.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC653
GMDN Code:
57548
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Prostate
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
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