Spectrin is a cytoskeletal protein which is found in muscles, red blood cells and red cell precursors. Anti-Spectrin antibody is useful in the identification of blood dyscrasias and muscle disorders.
Antibody Isotype:
IgG2b-k
Monosan Range:
MONOSAN
Clone:
RBC2/3D5
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Sadahira, Y et al. J Clin Pathol. 1999 Dec; 52(12): 919-21
References 2:
Nehls, V et al. Am J Pathol. 1993 May; 142(5): 1565-73
References 3:
Muller M et al. J Vet Med A Physiol Pathol Clin Med. 2001 Feb;48(1):51-7
References 4:
Terada N et al. J Anat. 1997 Apr;190(Pt 3):397-404
Smooth Muscle Myosin, heavy chain (SMMS-1) is a cytoplasmic structural protein that is a major component of the contractile apparatus of the smooth muscle cells. SMMS-1 is also a myoepitheliumassociated protein. Anti-SMMS-1 is a mouse monoclonal antibody to smooth muscle myosin, heavy chain that reacts with human visceral and vascular smooth muscle cells. The antibody also reacts with human myoepithelial cells. It is very helpful in distinguishing between benign sclerosing breast lesions and infiltrating carcinomas in difficult cases since it strongly stains the myoepithelial layer in the benign lesions while it is negative in the infiltrating carcinomas.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN
Clone:
SMMS-1
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Werling RW, et al. Am J Surg Pathol. 2003; 27:82-90
References 2:
Agoff SN, et al. Appl Immunohistochem Mol Morphol. 2001; 9:164-9
References 3:
Popnikolov NK, et al. Am J Clin Pathol. 2003; 120:161-7
References 4:
Lazard D, et al. Proc Natl Acad Sci USA. 1993; 90:999-1003
S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, Schwann cells, epithelial and myoepithelial cells of the breast, salivary and sweat glands, as well as in glial cells.1,2,6 Neoplasms derived from these cells also express S-100 protein, albeit non-uniformly.1-4 A large number of well differentiated tumors of the salivary gland, adipose and cartilaginous tissue, 3 and Schwann cell-derived tumors express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S-100 protein.4,5 Despite the fact that S-100 protein is an ubiquitous substance, its demonstration is of great value in the identification of several neoplasms, particularly melanomas.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
4C4.9
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Nakajima T, et al. Ad J Surg Path. 1982; 6:715-727
References 2:
Kuhn HJ, et al. Am J Clin Path. 1983; 79:341-347
References 3:
Yaziji H, et al. Int J Surg Pathol. 2003; 11:11-5
References 4:
Patel P, et al. J Am Acad Dermatol. 2002; 46:264-70
References 5:
Morrison CD, et al. Semin Diagn Pathol. 2000; 17:204-15
Anti-renal cell carcinoma (RCC) recognizes a 200 kD glycoprotein localized in the brush border of the proximal renal tubule. This antibody immunoreacts with approximately most primary renal cell carcinomas and can aid in the diagnosis when renal cell carcinoma enters the differential diagnosis. Other tumors that may react with this antibody are parathyroid adenoma, an occasional breast carcinoma. Nephroblastoma, oncocytoma, mesoblastic nephroma, transitional cell carcinoma, and angiomyolipoma are not labeled with this antibody.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN
Clone:
PN-15
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Dabbs, D. 4th Edition. Elsevier Saunders. 2014; p234
References 2:
Bakshi N, et al. Appl Immunohistochem Mol Morphol. 2007; 15:310-5
References 3:
McGregor DK, et al. Am J Surg Pathol. 2001; 25:1485-92
References 4:
Avery, AK et al. Am J Surg Pathol 24(2): 203-210, 2000
PU.1 is a transcription factor that has been shown to be important for normal B-cell development. PU.1 belongs to the ETS family of transcription factors. It is expressed in the myeloid lineage and in immature as well as mature B-lymphocytes, with the exception of plasma cells. PU.1 is essential during early B-cell differentiation. The absence of PU.1 results in total block of B-cell development at the prepro stage. Very little is known about PU.1 function in later stages of B-cell development. PU.1 does not seem to play a role in the end-stage of B-cell development and is not expressed in plasma cells. PU.1 exerts an important role in the regulation of the expression of crucial B-cell proteins, such as immunoglobulin (Ig) genes, and CD20 and its putative binding sites were also identified in the promoters of CD79, CD10, and CD22. PU.1 binds to the 3 enhancer region of both the Ig kappa and lambda light chain genes and it also regulates the immunoglobulin heavy chain genes through the intron enhancer region.
Antibody Isotype:
IgG
Monosan Range:
MONOSAN
Clone:
EPR3158Y
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
The antibody reacts with prostatic acid phosphatase in the glandular epithelium of normal and hyperplastic prostate, and adenocarcinoma of the prostate. Anti-PSAP is useful in identifying prostatic origin of tumors in the metastatic setting. PSAP complements other immunohistochemical markers in the correct clinical context.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
PASE/4LJ
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Ansari, MA, et al. Am J Clin Path 1981;76:94-98
References 2:
Kimura, N, et al. Virchows Arch A 1986;4:247-251
References 3:
Kidwai N et al. Breast Cancer Res. 2004;6(1):R18-23
References 4:
Genega EM et al. Mod Pathol. 2000 Nov;13(11):1186-91
References 5:
Gatalica Z et al. Appl Immunohistochem Mol Morphol. 2000 Jun;8(2):158-61
Prostate-Specific Antigen (PSA) is a 33 kDa protein primarily produced by the prostatic epithelium and the epithelial lining of the periurethral glands. PSA is strongly expressed in both normal and neoplastic prostatic tissue. Although PSA can be considered prostate-specific, PSA and/or PSA gene expression has been detected at low levels in some extra-prostatic tissues such as normal breast tissue, breast tumors, endometrium, adrenal neoplasms and renal cell carcinomas. Anti-PSA is most useful in determining the prostatic origin of carcinomas in non-prostate tissues (metastatic disease) using IHC techniques. This product is best used in conjunction with a panel of antibodies as, up to 27% of prostate carcinoma cases (predominantly poorly differentiated carcinomas) can be negative for this marker.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN
Clone:
ER-PR8
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Polascik TJ, et al. J Urol. 1999; 162:293
References 2:
Stenman UH, et al. Semin Cancer Biol. 1999; 9:83-93
References 3:
Alanen KA, et al. Path Res Pract. 1996; 192:233-237
The antibody reacts with progesterone receptor forms alpha and beta. This antibody stains nuclei in breast, ovarian and endometrial epithelia, as well as myometrial nuclei. Since the early 1990s the immunohistochemical (IHC) assay determination of progesterone receptor status has replaced the dextran-coated charcoal method as a prognostic indicator in breast carcinoma. IHC has shown to be superior in prognostic significance when using any one of several available methods of quantitation using this technique.
Antibody Isotype:
IgG
Monosan Range:
MONOSAN
Clone:
Y85
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Dabbs D. Diagnostic Immunohistochemistry, 2nd edition. p 728-32
References 2:
Dunnwald LK, et al. Breast Cancer Res. 2007;9(1):R6
References 3:
Leong A S-Y, et al. Manual of diagnostic immunohistochemistry, 2nd edition. p 375-76
Pneumocystis carinii is a fungal organism which is detected in human tissues (typically in lung in immunocompromised patients) in the trophozoite stage. Anti-Pneumocystis carinii antibody reacts with an epitope on the organism which is resistant to formalin, picric acid, paraffin, as well as alchohol and xylene. No cross-reactivity has been demonstrated with other fungi or parasitic organisms.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
3F6
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Anti-PLAP antibody immunoreacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease. This antibody has shown cross-reaction with human intestinal alkaline phosphatase.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN
Clone:
NB-10
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Paiva, J, et al. Am J Pathol 1984;111:156-165
References 2:
Burke, AP, et al. Hum Path 1988;19:663-670
References 3:
Wick, MR, et al. Hum Path 1987;18:946-954
References 4:
Saad RS et al. Appl Immunohistochem Mol Morphol. 2003 Jun;11(2):107-12
References 5:
Goldsmith JD et al. Am J Surg Pathol. 2002 Dec;26(12):1627-33
Perforin is a pore-forming protein that leads to osmotic lysis of the target cells and subsequently enables granzymes to enter the target cells and activate apoptosis, the cell death program. The expression of perforin is reportedly upregulated in activated CD8+ T-cells, natural killer cells and some CD4+ T-cells.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MRQ-23
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Chu PG, et al. Ann Diagn Pathol. 1999; 3:104-33
References 2:
Bittmann I, et al. Arch. 2004; 445:375-81
References 3:
dAmore ES, et al. Pediatr Dev Pathol. 2007; 10:181-91
Programmed death-1 (PD1) is a member of the CD28 family of receptors that includes CD28, cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4), inducible costimulator (ICOS), and B- and T-lymphocyte attenuator. These receptors play a role in the cellular immune response. For example, CD28 serves as a costimulatory receptor that enhances T-cell activation, whereas CTLA-4 serves as an inhibitor of T-cell activation. PD1 also has an inhibitory function on T cells and B cells, and is important in peripheral tolerance. There are at least 2 ligands for PD1, PD-L1, and PD-L2, which are expressed on a range of cells.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
NAT105
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Bolstad AI, et al. Arthritis Rheum. 2003 Jan;48(1):174-85
References 2:
Dorfman DM, et al. Am J Surg Pathol. 2006 Jul;30(7):802-10
References 3:
Hamanishi J, et al. Proc Natl Acad Sci U S A. 2007 Feb 27;104(9):3360-5
References 4:
Konishi J, et al. Clin Cancer Res. 2004 Aug 1;10(15):5094-100
References 5:
Mataki N, et al. Am J Gastroenterol. 2007 Feb;102(2):302-12
This antibody is an Analyte Specific Reagent (ASR). The antibody targets the capsid proteins VP1 and VP2 on Human Parvovirus. Parvovirus B19 infection has been implicated as a cause in spontaneous abortion in humans and thus application of this antibody to placental tissues in such cases is appropriate. Parvovirus B19 is also associated with erythema infectiosum (fifth disease) in children and acute arthritis in adults, as well as chronic hemolytic anemia, with some patients experiencing aplastic crisis.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
R92F6
Concentration:
n/a
Format:
Supernatant
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Loughrey AC et al. J Med Vir 39:97-100 (1993)
References 2:
Moore L et al. Med J Australia 159:344-345 (1993)
References 3:
Morey AL et al. J Path 166:105-108 (1992)
References 4:
ONeill HJ et al. 123: 125-134 (1992)
References 5:
Silverberg SG et al. Principles and Practice of Surgical Pathology and Cytopathology, 3rd edition. 1997; p. 219-220
The parathyroid glands function within the endocrine system to promote blood calcium homeostasis through controlled release of parathyroid hormone (PTH). This process involves the synthesis and secretion of PTH by activated parathyroid chief cells during conditions of hypocalcemia. With the anatomical proximity to the thyroid and capacity of associated neoplasms of the parathyroid to mimic thyroid tumors, challenges can arise in distinguishing between these types of abnormalities. In cases where there is uncertainty about a tumor being of parathyroid origin, immunohistochemical evaluation using anti-PTH can be of value.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
MRQ-31
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Aldinger KA, et al. Cancer; 49:388-97 (1982)
References 2:
Brown EM. Mineral Electrolyte Metal; 8:130-50 (1982)
References 3:
Abate EG, et al. Front Endocrinol (Lausanne).; 7:172 (2017)
References 4:
Duan K, et al. Turk Patoloji Derg.; 31 Suppl 1:80-97 (2015)
References 5:
Chen HL, et al. Journal of Biology and Chemistry; 277:19374-81 (2002)
The antibody has been used as an aid in discriminating complete hydatidiform mole (CHM) (no nuclear labeling of cytotrophoblasts or stromal cells) from partial hydatidiform mole (PHM) (nuclear staining of both cytotrophoblasts and stromal cells) and hydropic abortion. In normal placenta, cytotrophoblast, syncytio trophoblast, and stromal cells are labeled with this antibody. Intervillous trophoblastic islands demonstrate nuclear labeling in all entities and serve as an internal control.
Antibody Isotype:
IgG2b-k
Monosan Range:
MONOSAN
Clone:
Kp10
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Kihara M, et al. J Reprod Med. 2005; 50:307-12
References 2:
Romaguera RL, et al. Fetal Pediatr Pathol. 2004; 23:181-90
The antibody recognizes a 53 kDa phosphoprotein, identified as p53 suppressor gene product. It reacts with the mutant as well as wild type p53.1 Positive nuclear staining with this antibody has been shown to be a factor in breast carcinoma, lung carcinoma, colorectal carcinoma, urothelial carcinoma, and ependymoma.2-8 Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma, as well as a marker for intratubular germ cell neoplasia.
Antibody Isotype:
IgG2b-k
Monosan Range:
MONOSAN
Clone:
DO7
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Mauri FA et al. Int J Oncol 1999 Dec;15(6):1137-47
References 2:
Caffo O et al. Clin Cancer Res 1996 Sep;2(9):1591-9
References 3:
Bebenek M et al. Anticancer Res 1998 Jan-Feb;18(1B):619-23
References 4:
Midulla C et al. Anticancer Res 1999 Sep-Oct;19(5B):4033-7
References 5:
Moore BE et al. App.IHC and Mol. Morphol. 2001;9(3): 203 206
Alpha-Methylacyl-CoA Racemase (also known as AMACR or P504s) is an essential enzyme in the ?oxidation of branched-chain fatty acids. AMACR over-expression has been demonstrated in several cancers including colorectal, prostate, ovarian, breast, bladder, lung, and renal cell carcinomas, lymphoma, and melanoma. Staining with the antibody to this enzyme has been useful in identifying prostate carcinoma and prostatic intraepithelial neoplasia, as well as atypical adenomatous hyperplasia in formalin-fixed paraffinized tissue in morphologically difficult cases.
Antibody Isotype:
IgG
Monosan Range:
MONOSAN
Clone:
13H4
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Browne TJ et al. Hum Pathol. 2004 Dec;35(12):1462-8
References 2:
Wu CL et al. Hum Pathol. 2004 Aug;35(8):1008-13
References 3:
Evans AJ. J Clin Pthol. 2003 Dec;56(12):892-7
References 4:
Beach R, Gown AM, et al. Am J Surg Pathol. 2002 Dec;26(12):1588-96
References 5:
Jiang Z, Wu CL, et al. Am J Surg Pathol. 2002 Sep;26(9):1169-74
p27, also known as cyclin-dependent kinase inhibitor 1B (CDNK1B), is a kinase inhibitor that controls cell cycle progression.1-4 p27 is involved in G1 phase arrest and obstructs cell entry into the S phase by binding to and inhibiting cyclin E-CDK2, effectively slowing or stopping the cell division cycle.1-4 p27 is broadly expressed in normal tissue but can be dysfunctional in neoplastic tissue and, therefore, not expressed.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN
Clone:
SX53G8
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
p21 is one of the inhibitors of the phosphorylation of the cyclin-cdk complex. p21, which is an inhibitor of G1 cdks, suppresses the cell cycle and inhibits DNA synthesis. Although p21 is induced by p53 and inhibits cdk (cyclin-dependent kinase) activity, there was virtually no correlation between the expression of p21 and that of p53; this finding was consistent with two reports, though another reported an inverse correlation between the expression of p21 and that of p53. p53independent expression of p21 might account for the discrepancy between the expression of p53 and that of p21. It is expressed in normal human tissue and a wide array of tumors.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN
Clone:
DCS-60.2
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Nerve growth factor receptor (NGFR), also known as p75NTR, is a 75-kDa glycoprotein member of the tumor necrosis factor (TNF) receptor family essential for embryonic development of the peripheral nervous system. In normal tissue, NGFR is expressed in neural crest derived cells, lymphoid follicular dendritic cells seen in lymph nodes and tonsils, and myoepithelial cells of the breast, prostate, and salivary gland as well as basal epithelium of the respiratory system. NGFR has been shown to be a reliable adjunct marker for melanoma, specifically desmoplastic and spindle cell variants Anti-NGFR labels myoepithelial cells of the breast and may aid in the differentiation between benign conditions, pre-invasive neoplastic lesions and invasive malignancies of the breast.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MRQ-21
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Thompson SJ. Am J Clin Pathol. 1989; 92:415-23
References 2:
Reis-Filho JS, et al. Mod Pathol. 2006; 19:307-19
References 3:
Lazova R, et al. J Am Acad Dermatol. 2010; 63:852-8
Myogenin also identified as myogenic factor 4 is a muscle specific transcription factor associated with muscle differentiation and cell cycle.1 Anti-myogenin reactivity is seen in the nuclei of myoblasts in developing muscle tissue. Anti-myogenin is a useful immunohistochemical reagent for identification of rhabdomyosarcoma.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN
Clone:
F5D
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Miller JB. J Cell Biol.; 111:1149-59 (1990)
References 2:
Wang NP, et al. Am J Pathol.; 147:1799-810 (1995)
References 3:
Cui S, et al. Pathol Int.; 49:62-8 (1999)
References 4:
Kaspar et al. Sci Rep.; 5:15090 (2015)
References 5:
Rudzinski et al. Am J Surg Pathol.; 38:654-9 (2014)
The antibody labels a 50kDa, multiple myeloma oncogen-1 (MUM1) protein. MUM1 is encoded by the MUM1/IRF-4 gene, which is mapped to 6q23-25 and identified as a myeloma-associated oncogene. It is a member of the interferon regulatory factor family of transcription factors and plays an important role in the regulation of gene expression in response to signaling by interferon and other cytokines. MUM1 positive cells express the protein in the nucleus in a diffuse and microgranular pattern. However, some positivity is also observed in the cytoplasm of MUM1-expressing cells. In normal/reactive lymphoid tissues, such as lymph node, this antibody stains plasma cells, some B-cells in the light zone of germinal centers, and a subset of T-cells (T-cells in germinal centers and interfollicular areas). MUM1 expression has been described in diffuse large B-cell lymphoma (DLBCL). MON 3318 can stain other Bcell lymphomas such as lymphoplasmacytic lymphoma, chronic lymphocytic leukemia, follicular lymphoma, marginal zone lymphoma, lymphoblastic lymphoma/leukemia, primary effusion lymphoma, DLBCL, Burkitt-like lymphoma, and classical Hodgkin lymphoma.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MRQ-8
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Falini B, et al. Blood. 2000; 95:2084-92
References 2:
Grossman A, et al. Genomics. 1996; 37:229-33
References 3:
Neresh KN. Haematologica. 2007; 92:267-8
References 4:
Van Imhoff GW, et al. J Clin Oncol. 2006; 24:4135-42
References 5:
Gualco G, et al. Appl Immunohistochem Mol Morphol. 2010; 18:301-10
Mucins are high molecular weight glycoproteins which constitute the major component of the mucus layer that protects the gastric epithelium from chemical and mechanical aggressions. In humans, at least 14 mucin genes have been identified that code for the mucin proteins. MUC6 is a secretory mucin that is part of a family of at least 14 high molecular weight glycoproteins made by many epithelial tissues. MUC6 is preferentially expressed in non-neoplastic gastric tissue, specifically in the pyloric glands. During neoplastic transformation, mucin expression may be altered within these tissues leading to particular patterns of expression.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MRQ-20
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Ruco LP, et al., Am J Clin Pathol. 92:273-9 (1989)
References 2:
Facchetti F, et al., Histopathology. 19:141-5 (1991)
References 3:
Do SI. et al. J of Breast Cancer. 16:152-8 (2013)
References 4:
Vergier B et al. Blood., 9597: 2212-8 (2000)
References 5:
Mino-Kenudson M, et al. Virchows Arch. 469:255-65 (2016)
Mucins are high molecular weight glycoproteins which constitute the major component of the mucus layer that protects the gastric epithelium from chemical and mechanical injury. In humans, at least 14 mucin genes have been identified that code for the mucin proteins. Reportedly, mucin expression is associated with tumor type of gastric carcinomas, with MUC2 being associated with mucinous carcinomas. Anti- MUC2 reactivity is seen in goblet cells of the small intestine and colon, and it is useful in immunohistochemistry for identifying colonic, gastric and esophageal carcinomas.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MRQ-18
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Chaves P, et al. Dis Esophagus. 2005; 18:383-7
References 2:
Leteurtre E, et al. World J Gastroenterol. 2006; 12:3324-31.
References 3:
Mino-Kenudson M, et al. Arch Pathol Lab Med. 2007; 131:86-90
References 4:
Mizoshita T, et al. Histol Histopathol. 2007; 22:251-60
References 5:
OConnell FP, et al. Arch Pathol Lab Med. 2005; 129:338-47
Mucins are high molecular weight glycoproteins which constitute the major component of the mucus layer that protects the gastric epithelium from chemical and mechanical injury. In humans, at least 14 mucin genes have been identified that code for the mucin proteins. Mucin genes are expressed in a regulated cell- and tissue-specific manner. The stomach provides a good example of such differential expression of mucin genes. MUC1 is detected in mucous cells of the surface epithelium and neck region of the gastric antrum, as well as in pyloric glands and oxyntic glands of the body region. The heterogeneous pattern of mucin expression, including the expression of the intestinal mucin MUC2, may provide new insights into the differentiation pathways of gastric carcinoma.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MRQ-17
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Chaves P, et al. Dis Esophagus. 2005; 18:383-7
References 2:
Leteurtre E, et al. World J Gastroenterol. 2006; 12:3324-31.
References 3:
Mino-Kenudson M, et al. Arch Pathol Lab Med. 2007; 131:86-90
References 4:
Mizoshita T, et al. Histol Histopathol. 2007; 22:251-60
References 5:
OConnell FP, et al. Arch Pathol Lab Med. 2005; 129:338-47
MSH6 is a mismatch repair gene which is deficient in a high proportion of patients with microsatellite instability (MSI-H). This finding is associated with the autosomal dominant condition known as Hereditary Non-Polyposis Colon Cancer (HNPCC). The anti-MSH6 antibody is useful in screening patients and families for this condition. Colon cancers that are microsatellite unstable have a better prognosis than their microsatellite stable counterparts.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
44
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Lagerstedt Robinson K, et al. J Natl Cancer Inst. 2007 Feb 21;99(4): 291-9
References 2:
Niessen RC et al. Gut 2006 Dec;55(12):1781-8
References 3:
Lawes DA et al. Br J Cancer. 2005 Aug 22; 93(4):472-7
References 4:
Stormorken AT et al. J Clin Oncol. 2005 Jul 20;23(21):4705-12
References 5:
Rigau V et al. Arch Pathol Lab Med. 2003;127:694-700
MSH2 is a mismatch repair gene which is deficient in a high proportion of patients with microsatellite instability (MSI-H). This finding is associated with the autosomal dominant condition known as Hereditary Non-Polyposis Colon Cancer (HNPCC). The anti-MSH2 antibody is useful in screening patients and families for this condition. Colon cancers that are microsatellite unstable have a better prognosis than their microsatellite stable counterparts.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
G219-1129
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Christensen M et al. Cancer 2002;95: 2422-30
References 2:
Wright CL et al. Am J Surg Pathol. 2003;27: 1393-1406
References 3:
Renkonen E et al. J Clin Oncol 2003; 21: 3629-3637
References 4:
Hoedema R. et al. The American Surgeon 2003, May 69(5): 387-92
References 5:
Brueckl WM et al. Anticancer Research 2003; 23: 1773-1778
MLH1 is a mismatch repair gene that is deficient in a high proportion of patients with microsatellite instability (MSI-H). This finding is associated with the autosomal dominant condition known as Hereditary Non-Polyposis Colon Cancer (HNPCC). The anti-MLH1 antibody is useful in screening patients and families for this condition. Colon cancers that are microsatellite unstable have a better prognosis than their microsatellite stable counterparts
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
G168-728
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Christensen M et al. Cancer 2002;95: 2422-30
References 2:
Wright CL et al. Am J Surg Pathol. 2003;27: 1393-1406
References 3:
Renkonen E et al. J Clin Oncol 2003; 21: 3629-3637
References 4:
Hoedema R. et al. The American Surgeon 2003, May 69(5): 387-92
References 5:
Brueckl WM et al. Anticancer Research 2003; 23: 1773-1778
Mi is a transcription factor implicated in pigmentation, bone development and in mast cells. Various forms of Mi exist ranging from 50-70 kD in size. This antibody targets the 52-56 kD range. This antibody has been useful in identifying malignant melanoma.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
C5/D5
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Liegl B, et al. Am J Surg Pathol. 2008; 32:608-14
References 2:
Righi A, et al. Int J Surg Pathol. 2008; 16:16-20
References 3:
Weinreb I, et al.. Virchows Arch. 2007; 450:463-70
References 4:
Ohsie SJ, et al. J Cutan Pathol. 2008; 35:433-44
References 5:
Hornick JL, et al. Am J Surg Pathol. 2008; 32:493-501
Hector Battifora mesothelial-1 (HBME-1) is a membrane antigen that exists in the microvilli of mesothelial cells and other epithelial cells. Anti-HBME-1 labels thyroid papillary carcinoma and follicular carcinoma but not normal thyroid making it a valuable marker for distinguishing thyroid malignacies from benign thyroid lesions. It has also been demonstrated to label mesothelial cells, both be
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
HBME-1
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Coli A, Bigotti G, et al. J Exp Clin Cancer Res. 2007 Jun;26(2):221-7
Anti-HMB45 is a useful melanoma immunohistochemical marker that reacts with antigens present on immature melanosomes. Anti-HMB45 is useful for identifying amelanotic melanoma from other neoplastic lesions with similar morphology.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN
Clone:
HMB-45
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Gown AM, et al. Am J Pathol. 1986; 123:195-203
References 2:
Wick MR, et al. Arch Pathol Lab Med. 1988; 112:616-20
References 3:
Abrahamsen HN, et al. Cancer. 2004; 100:1683-91
References 4:
Vaggelli L, et al. Tumori. 2000; 86:346-8
References 5:
Baisden BL, et al. Am J Surg Pathol. 2000; 24:1140-6
The antibody s useful as an immunohistochemical reagent to stain melanocytes and tumors derived therefrom. Anti-PNL2 reactivity is identified in the cytoplasm of cutaneous and oral mucosal melanocytes. Anti-PNL2 labels intraepidermal nevi, while the dermal components of compound nevi are largely non-reactive.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
PNL2
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
The antibody is a monoclonal, anti-melanoma antibody that reacts with an antigen that has yet to be identified.1 Notably used as a melanoma marker, KBA.62 also detects smooth muscle, basal cells of the epidermis and hair shaft epithelia of the skin.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
KBA.62
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Pages C, et al.. Hum Pathol. 2008; 39:1136-42
References 2:
Aung P, et al. Am J Surg Pathol. 2012; 36:265-72
References 3:
E Cohen-Knafo et al. J Clin Pathol. 1995; 48:826-831
References 4:
Kaufmann O, et al.. Mod Pathol, 1998 Aug; 11(8):740-6
MART-1 (also known as Melan A) is a melanocyte differentiation antigen. MART-1 is a transmembrane protein present in melanocytes of normal skin, retina, nevi, and most melanomas. MART-1 is a very useful marker for identifying metastatic melanomas.
Antibody Isotype:
IgG2b-k
Monosan Range:
MONOSAN
Clone:
M2-7C10
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Kageshita T et al. J Immunother 1997 Nov;20(6):460-5
References 2:
Yaziji H, et al. In J Surg Pathol. 2003 Jan;11(1):11-5
References 3:
Suchak R, et al. . Am J Dermatopathol. 2014; 36:387-91.
References 4:
Helm K et al. J Cutan Pathol. 2008; 35:931-4
References 5:
Nielsen PS, et al. Am J Dermatopathol. 2011; 33:361-70
MART-1 (also known as Melan A) is a melanocyte differentiation antigen. MART-1 is a transmembrane protein present in melanocytes of normal skin, retina, nevi, and most melanomas. MART-1 is a very useful marker for identifying metastatic melanomas.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
A103
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Kageshita T et al. J Immunother 1997 Nov;20(6):460-5
References 2:
Yaziji H, et al. In J Surg Pathol. 2003 Jan;11(1):11-5
References 3:
Mocellin S et al. J Immunother. 2001 Nov-Dec;24(6):447-58
References 4:
Perez RP et al. Hum Pathol. 2000 Nov;31(11):1381-8
References 5:
Hoang MP et al. J Cutan Pathol. 2001 Sep;28(8):400-6
Mammaglobin is breast-associated glycoprotein distantly related to secretoglobin family that includes human uteroglobin and lipophilin. Anti-mammaglobin labels cytoplasm of normal breast epithelial cells as well as primary and metastatic breast carcinomas. Absence of mammaglobin expression is typically seen in prostate, kidney, colon, rectum, small intestine, stomach, pancreas, lung and thyroid tissue.2,5 Mammaglobin may be used as part of an immunohistochemical panel for determination of metastatic breast carcinoma and tumor of unknown primary origin.
Antibody Isotype:
IgG1, IgG
Monosan Range:
MONOSAN
Clone:
304-1A5/31A5
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Fleming TP et al. Ann NY Acad Sci 2000;923:78-89
References 2:
Bhargava R, et al. Am J Clin Pathol. 2007; 127:103-13
References 3:
Wang Z, et al. Int J Clin Exp Pathol. 2009; 2:384-9
References 4:
Han JH, et al. Arch Pathol Lab Med. 2003; 127:1330-4
Mammaglobin is breast-associated glycoprotein distantly related to secretoglobin family that includes human uteroglobin and lipophilin. Anti-mammaglobin labels cytoplasm of normal breast epithelial cells as well as primary and metastatic breast carcinomas. Absence of mammaglobin expression is typically seen in prostate, kidney, colon, rectum, small intestine, stomach, pancreas, lung and thyroid tissue.2,5 Mammaglobin may be used as part of an immunohistochemical panel for determination of metastatic breast carcinoma and tumor of unknown primary origin.
Antibody Isotype:
IgG
Monosan Range:
MONOSAN
Clone:
31A5
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Fleming TP et al. Ann NY Acad Sci 2000;923:78-89
References 2:
Bhargava R, et al. Am J Clin Pathol. 2007; 127:103-13
References 3:
Wang Z, et al. Int J Clin Exp Pathol. 2009; 2:384-9
References 4:
Han JH, et al. Arch Pathol Lab Med. 2003; 127:1330-4
Blood group Lewis carbohydrate determinants are oligosaccharides on glycolipids and glycoproteins. In healthy individuals the LewisY antigen is a type 2 antigen usually only expressed in low levels of a few cell types such as some epithelial cells. Reportedly these antigens are aberrantly expressed in high levels in many carcinomas. Anti-BG8, LewisY reactivity in immunohistochemistry is seen in carcinomas of the breast, lung, and colon.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
F3
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Davidson B, et al.. Virchows Arch. 1999; 435:43-9
References 2:
King JE, et al. Histopathology. 2006; 48:223-32
References 3:
Marchevsky AM et al. Appl Immunohistochem Mol Morphol. 2007; 15:140-4
The antibody detects surface immunoglobulin on normal and neoplastic B-cells. Anti-lambda staining is seen in B-cell follicles of human lymphoid tissue. When dealing with B-cell neoplasms, the determination of light chain ratios remains helpful. Most B-cell lymphomas express either kappa or lambda light chains, whereas reactive proliferations display a mixture of kappa and lambda positive cells. If only a single light chain type is detected, a lymphoproliferative disorder is very likely.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
Lamb14
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Hertel, BF, et al. Lab Invest 1977;36:12
References 2:
Taylor, CL Arch Pathol Lab Med 1978;12:113-121
References 3:
Abbondanzo SL et al. Ann Diagn Pathol. 1999 Oct;3(6):394
References 4:
Kurtin PJ et al. Am J Clin Pathol. 1999 Sep;112(3):319-29
References 5:
Ashton-Key M et al. Histopathology. 1996 Dec;29(6):525-31
Kidney-specific cadherin (Ksp-cadherin) is a member of the cadherin family of cell adhesion molecules that is found exclusively in the basolateral membrane of renal tubular epithelial cells of the distal tubules and collecting duct. Ksp-cadherin may be useful in distinguishing between renal neoplasms of distal nephron origin from those of proximal tubule origin.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MRQ-33
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
The antibody detects surface immunoglobulin on normal and neoplastic B-cells. In paraffin-embedded tissue, anti-kappa exhibits strong staining of kappa-positive plasma cells and cells that have absorbed exogenous immunoglobulins. When dealing with B-cell neoplasms, the determination of light chain ratios remains the centerpiece. Most B-cell lymphomas express either kappa or lambda light chains, whereas reactive proliferations display a mixture of kappa and lambda positive cells. If only a single light chain type is detected, a lymphoproliferative disorder exists. Monoclonality is determined by a kappa-lambda ratio of greater than or equal to 3:1 or a lambda-kappa ratio greater than 2:1.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN
Clone:
L1C1
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Hertel, BF, et al. Lab Invest 1977;36:12
References 2:
Mendes S, Dreno B. Acta Derm Venereol. 2003;83(3):167-70
References 3:
Lee LA et al. Am J Otolaryngol. 2002 Sep-Oct;23(5):316-20
References 4:
Taylor, CL Arch Pathol Lab Med 1978;12:113-121
References 5:
Schmid U et al. Am J Surg Pathol. 1995 Jan;19(1):12-20
The INI-1 gene, which encodes a functionally uncharacterized protein component of the hSWI/SNF chromatin remodeling complex, is often mutated or deleted in malignant rhabdoid tumor (MRT). Two isoforms of INI-1 that differ by the variable inclusion of amino acids are potentially produced by differential RNA splicing. The morphology of MRTs can present challenges in differential diagnosis. The overall survival of MRTs relative to its potential mimics [medulloblastoma, supratentorial primitive neuroectodermal tumors (sPNETs)] is quite low, and thus differentiation from these other tumors is desirable. Lack of nuclear labeling by anti-INI-1 is characteristic of MRT. The majority of medulloblastomas and sPNETs are labeled by anti-INI-1. MRTs also originate from the kidney and soft tissues.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
MRQ-27
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Bourdeaut F, et al. J Pathol. 2007; 211:323-30
References 2:
Fowler DJ, et al. Fetal Pediatr Pathol. 2006; 25:159-68
References 3:
Haberler C, et al. Am J SurgPathol. 2006; 30:1462-8
References 4:
Janson K, et al. Pediatr Blood Cancer. 2006 Sep:47(3):279-84
Inhibin is a peptide hormone that inhibits FSH secretion from the pituitary. Inhibin is a dimer that consists of an alpha and beta subunit. In normal tissue, anti-inhibin alpha labels granulosa cells of the ovary, sertoli and leydig cells of the testis, and the zona reticularis of the adrenal cortex. Anti-inhibin alpha has demonstrated utility in the identification of sex cord stromal tumors and adrenal cortical tumors.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
R1
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Stewart CJ, et al. Histopathology. 1997; 31:67-74
References 2:
McCluggage WG, et al. J Clin Pathol. 1998; 51: 114-6
References 3:
Kommoss F, et al. Mod Pathol. 1998; 11:656-64
References 4:
Guerrieri C, et al. Int J Gynecol Pathol. 1998; 17:266-71
Human herpesvirus type 8 (HHV-8) is the likely etiological agent of Kaposis sarcoma (KS). HHV-8 DNA sequences have been found in Kaposis sarcoma lesions, primary effusion lymphoma, and multicentric Castlemans disease via polymerase chain reaction and in situ hybridization. Latent nuclear antigen (LNA1, LNA, LANA-1), also known as ORF73, is a 222- or 234 kD protein that is consistently expressed in HHV8 infected cells. Anti-HHV-8 labels the latent nuclear antigen protein via immunohistochemistry
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
13B10
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Corbellino M et al AIDS Res Hum Retroviruses.;12(8):651-7 (1996)
References 2:
Schwartz EJ et al. Am J Surg Pathol.;27(12):1546-50 (2003)
References 3:
Boulanger E et al. Am J Hematol.;76(1):88-91 (2004)
References 4:
Courville P et al. Ann Pathol.;22(4):267-76 (2002)
Anti-hepatocyte specific antigen, also known as anti-Hep-Par1, recognizes both benign and malignant liver-derived tissues including such tumors as hepatocellular carcinoma. It recognizes both normal adult and fetal liver tissue. The typical pattern is a granular cytoplasmic staining. This antibody is useful in differentiating hepatocellular carcinomas with adenoid features from adenocarcinomas, either primary in the liver or metastatic lesions to the liver
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN
Clone:
OCH1E5
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Minervini MI, et al. Mod Pathol. 1997; 10:686-92
References 2:
Chu PG, et al. Am J Surg Pathol. 2002; 26:978-88
References 3:
Wieczorek T, et al. Am J Clin Pathol. 2002; 118:911-21
References 4:
Fasano M, et al. Mod Pathol. 1998; 11:934-8
References 5:
Maitra A, et al. Am J Clin Pathol. 2001; 115:689-94
Hemoglobin alpha chain belongs to the globin family and is involved in oxygen transport from the lung to the various peripheral tissues. Hemoglobin A is comprised of two alpha chains and two beta chains. Immunohistochemical localization of hemoglobin is excellent as an erythroid marker for the detection of immature, dysplastic, and megaloblastic erythroid cells in myeloproliferative disorders, such as erythroleukemia. In contrast, myeloid cells, lymphoid cells, plasma cells, histiocytes, and megakaryocytes do not stain with anti-hemoglobin A.
Antibody Isotype:
IgG
Monosan Range:
MONOSAN
Clone:
EPR3608
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
OMalley DP, et al. Mod Pathol. 2005; 18:1550-61
References 2:
Cherie H Dunphy, et al. Appl Immun Mol Morphol, 2005; 15(2):154-159
Glypican-3 (GPC3) is a membrane-bound heparin sulfate proteoglycan known to participate in cell growth and differentiation. GPC3 expression has been observed in the majority of hepatocellular carcinomas (HCC), but is rarely expressed in non-neoplastic hepatic tissue, making it a useful marker for HCC. Additionally, this marker is expressed in many yolk sac tumors.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
1G12
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Kandil DH, et al. Adv Anat Pathol. 2009; 16:125-9
References 2:
Coston WMP, et al. Am J Surg Pathol. 2008; 32:433-44
References 3:
Capurro M, et al. Gastroenterology. 2003; 125:89-97
References 4:
Zynger DL, et al. Am J Surg Pathol. 2006; 30:1570-5
Glycophorins A and B are major sialoglycoproteins expressed across the surface of the human erythrocyte membrane and contain the antigenic determinants that define the MNS blood group system.1 The high sialic acid content of glycophorin A contributes to the generation of a net negative surface charge across erythrocyte membranes that minimizes interactions between red blood cells and prevents their aggregation. Anti-glycophorin A has utility in identifying cells of the erythroid lineage.
Antibody Isotype:
IgG2b-k
Monosan Range:
MONOSAN
Clone:
GA-R2 (HIR2)
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Reid ME et al., Immunohematol 2009;25:95-101
References 2:
Olsen RJ et al. Arch Pathol Lab Med 2008; 132:462-75
The antibody detects astrocytes, Schwann cells, satellite cells, enteric glial cells, and some groups of ependymal cells. This marker is mainly used to distinguish neoplasms of astrocytic origin from other neoplasms in the central nervous system.
Antibody Isotype:
IgG
Monosan Range:
MONOSAN
Clone:
EP672Y
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Jessen, KR, et al. J Neurosci 1983;3:2206-2218
References 2:
Regner A et al. J Neurotrauma. 2001 Aug;18(8):783-92
References 3:
Nagashima G et al. Clin Neurol Neurosurg. 2002 May;104(2):125-31
References 4:
Choi, BH, et al. Science 1984;223:407-409
References 5:
Viale, G, et al. Virchows Arch A Pathol Anat 1991;418:339-348
GCDFP-15 is a glycoprotein localized in the apocrine metaplastic epithelium lining breast cysts and in apocrine glands in the axilla, vulva, eyelid, ear canal, and in salivary glands. GCDFP-15 positivity is seen in breast carcinomas. On the other hand, colorectal carcinomas, lung carcinoma, mesotheliomas rarely stain with this antibody. Because of its specificity for breast carcinoma, this antibody is often helpful in distinguishing metastasis of unknown primary.
Antibody Isotype:
IgG
Monosan Range:
MONOSAN
Clone:
EP1582Y
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Mazoujian G, et al. Am J Pathol. 1983; 110:105-12
References 2:
Liegl B, et al. Histopathology. 2007; 50:439-47
References 3:
Bhargava R, et al. Am J Clin Pathol. 2007; 127:103-13
References 4:
Tornos C, et al. Am J Surg Pathol. 2005; 29:1482-9
References 5:
Takeda Y, et al. Arch Pathol Lab Med. 2008; 132:239-43
GCDFP-15 is a 15 kD glycoprotein which is localized in the apocrine metaplastic epithelium lining breast cysts and in apocrine glands in the axilla, vulva, eyelid, ear canal, and in salivary glands. GCDFP-15 positivity is seen in breast carcinomas. On the other hand, colorectal carcinomas, lung carcinoma, mesotheliomas rarely stain with this antibody. Because of its specificity for breast carcinoma, this antibody is often helpful in distinguishing metastasis of unknown primary.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
23A3
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Mazoujian G, et al. Am J Pathol. 1983; 110:105-12
References 2:
Liegl B, et al. Histopathology. 2007; 50:439-47
References 3:
Bhargava R, et al. Am J Clin Pathol. 2007; 127:103-13
References 4:
Tornos C, et al. Am J Surg Pathol. 2005; 29:1482-9
References 5:
Takeda Y, et al. Arch Pathol Lab Med. 2008; 132:239-43
Galectin-3 is a 30-kD protein, a member of the beta-galactosidase-binding lectin family. Galectin-3 is associated with cell growth, adhesion, inflammation, mRNA processing, and apoptosis. Reportedly, Galectin-3 aberrant expression is related to malignant transformation and metastasis in carcinomas of the breast, colon and thyroid. Galectin-3 reactivity can be seen in the nucleus of neutrophils, vascular endothelium, carcinomas of the colon, breast, and thyroid. Galectin-3 may be useful in the differentiation of benign and malignant thyroid neoplasms. Galectin-3 may also be useful in the identification of certain liver disorders.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
9C4
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Orlandi F, et al. Cancer Res. 1998; 58:3015-20
References 2:
Bartolazzi A, et al. Lancet. 2001; 357:1644-50
References 3:
Papotti M, et al. Eur J Endocrinol. 2002; 147: 515-21
Friend leukemia integration 1 transcription factor (FLI-1) is a protein encoded by the proto-ocogene FLI1. The FLI-1 protein is best known for its critical role in the pathogenesis of ES/pPNET. FLI-1 is normally expressed in endothelial cells and in hematopoietic cells, including T-lymphocytes. The immunohistochemical detection of FLI-1 protein has been shown in studies to be valuable in the discrimination of Ewing sarcoma/peripheral primitive neuroectodermal tumor (ES/pPNET) from most of its potential mimics. ES/pPNET is a rare primary tumor of the bone/soft tissue that resembles other undifferentiated tumors.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
MRQ-1
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Mhawech-Fauceglia P, et al. Histopathology. 2006; 49:569-75
References 2:
Kuroda N, et al. Med Mol Morphol. 2006; 39:221-5
References 3:
Ellison DA, et al. Hum Pathol. 2007; 38:205-11
References 4:
Blind C. et al. . J Clin Pathol. 2008 Jan;61(1):79-83. Epub 2007 Apr 5
Fascin is a 55-kd actin bundling protein involved in cell migration. Fascin is up-regulated in many human carcinomas and numerous studies have correlated Fascin over-expression with increased metastatic potential. Fascin is highly sensitive for staining Reed-Sternberg cells making it an excellent marker for classic Hodgkins lymphoma. It is uniformly negative in lymphoid cells, plasma cells and myeloid cells. MON 3279 is positive in dendritic cells. This marker may be helpful in distinguishing between Hodgkins disease and non-Hodgkins lymphoma in difficult cases.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
55-k2
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Chu Pg Ann Diagn Pathol. 1999;3(2):104-33
References 2:
Pelosi G et al. Lung Cancer. 2003;42(2):203-13
References 3:
Goncharuk VN et al. J Cutan Pathol. 2002;29(7):430-8
References 4:
Kraus MD et al. Am J Dermatopathol. 2001;23(2):104-11
Factor XIIIa has been identified in platelets, megakaryocytes, and fibroblast-like mesenchymal or histiocytic cells in the placenta, uterus, and prostate, monocytes and macrophages and dermal dendritic cells. Anti- Factor XIIIa has been found to be useful in differentiating between dermatofibroma (almost all cases +), dermatofibrosarcoma protuberans (-/+) and desmoplastic malignant melanoma. Anti-factor XIIIa positivity is also seen in capillary hemagioblastoma, hemangioendothelioma, hemangiopericytoma, xanthogranuloma, xanthoma, hepatocellular carcinoma, glomus tumor, and meningioma.
Antibody Isotype:
IgG
Monosan Range:
MONOSAN
Clone:
EP3372
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Nemes Z, Hum Pathol 1992 Jul; 23(7):805-10
References 2:
Horenstein MG et al. Am J Surg Pathol. 2000 Jul;24(7):996-1003
References 3:
Kraus MD et al. Am J Dermatopathol. 2001 Apr;23(2):104-11
References 4:
Abenoza P, et al. Am J Dermatopathol. 1993; 15:429-34
References 5:
Anstey A, Cerio R, et al.: Am J Dermatopathol 1994 Feb: 16(1):14-22
Factor XIIIa has been identified in platelets, megakaryocytes, and fibroblast-like mesenchymal or histiocytic cells in the placenta, uterus, and prostate, monocytes and macrophages and dermal dendritic cells. Anti-factor XIIIa has been found to be useful in differentiating between dermatofibroma (almost all cases +), dermatofibrosarcoma protuberans (-/+) and desmoplastic malignant melanoma. Anti-factor XIIIa positivity is also seen in capillary hemagioblastoma, hemangioendothelioma, hemangiopericytoma, xanthogranuloma, xanthoma, hepatocellular carcinoma, glomus tumor, and meningioma.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
AC-1A1
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Nemes Z, Hum Pathol 1992 Jul; 23(7):805-10
References 2:
Horenstein MG et al. Am J Surg Pathol. 2000 Jul;24(7):996-1003
References 3:
Kraus MD et al. Am J Dermatopathol. 2001 Apr;23(2):104-11
References 4:
Dehner LP. Am J Surg Pathol. 2003 May;27(5):579-93
References 5:
Deguchi M et al. Arch Dermatol Res. 2002 Oct;294(7):297-302
The antibody 4 targets the 60 kDa latent membrane protein (LMP-1) encoded by the BNLF1 gene of the Epstein-Barr virus. There is cross-reactivity with Reed Sternberg cells of Hodgkins disease. The EpsteinBarr virus is an important as a cause of Infectious mononucleosis and has been associated with oral carcinomas.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
CS1-4
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Murray PG et al. J Pathol. 166: 1-5 (1992)
References 2:
Jarrett RF et al. Blood 78:1-10 (1991)
References 3:
Pailesen G et al. Lancet. 337: 320-322 (1991)
References 4:
Silverberg GS et al Principles and Practice of Surgical Pathology and Cytopathology, 3rd edition. (1997)
Epithelial cell adhesion molecule (Ep-CAM) is a transmembrane glycoprotein localized on the membrane of cells in most epithelial tissues. Immunoreactivity with the antibody to Ep-CAM has been seen in the majority of epithelial neoplasms, whereas most non-epithelial neoplasms do not show EpCAM expression. Ep-CAM is not expressed in mesothelial cells, hepatocytes, and lymphocytes. In conjunction with other markers, Ep-CAM can be used as an aid in determining neoplasms of epithelial origin, such as distinguishing between lung adenocarcinoma and mesothelioma.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN
Clone:
Ber-EP4
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Schnell U, et al. Biochim Biophys Acta. 2013; 1828:1989-2001
The antibody s a useful marker for staining many carcinomas. It stains normal and neoplastic cells from various tissues, including mammary epithelium, sweat glands and colorectal carcinoma. Hepatocellular carcinoma, adrenal carcinoma and embryonal carcinomas are consistently EMA negative, so keratin positivity with negative EMA favors one of these tumors. EMA is frequently positive in meningioma, which can be useful when distinguishing it from other intracranial neoplasms such as schwannomas.
Antibody Isotype:
IgG2a-k, IgG1-k
Monosan Range:
MONOSAN
Clone:
E29
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Dearnaly DP, et al. Lancet 1983; 1271-4
References 2:
Attanoos RL, et al. Histopathology. 2003; 43:231-8
E-cadherin is an adhesion protein that is expressed in cells of epithelial lineage. Anti-E-cadherin stains positively in glandular epithelium as well as adenocarcinomas of the lung, gastrointestinal tract and ovary. Another application involves the differentiation of ductal (which is membrane staining) vs. lobular cancer of the breast (which is cytoplasmic staining). It has also been shown to be positive in some thyroid carcinomas. A combination of E-cadherin and p120 catenin helps distinguish ductal carcinoma of the breast from lobular carcinoma.
Antibody Isotype:
IgG
Monosan Range:
MONOSAN
Clone:
EP700Y
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Han AC, et al. Hum Pathol. 1997; 28:641-5
References 2:
Simsir A, et al. Diagn Cytopathol. 1999; 20:125-30
References 3:
Abutaily AS, et al. J Clin Pathol. 2002; 55:662-8
References 4:
Acs G, et al. Am J Clin Pathol. 2001; 115:85-98
References 5:
Dabbs DJ, et al. Am J Surg Pathol. 2007; 31:427-37
Podoplanin is a transmembrane mucoprotein (38 kD) recognized by the D2-40 monoclonal antibody. Podoplanin is selectively expressed in lymphatic endothelium as well as lymphangiomas, and Kaposi sarcomas. Podoplanin has also been shown to be expressed in epithelioid mesotheliomas and seminomas.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN
Clone:
D2-40
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Ordóñez NG. Adv Anat Pathol. 2006; 13:83-8
References 2:
Niakosari F, et al. Arch Dermatol. 2005; 141:440-4
This Anti-cytomegalovirus antibody cocktail reacts with a two different epitopes. The DDG9 antibody reacts with a 76 kDa protein produces by CMV. CCH2 antibody reacts with the early DNA-binding protein p52. There is no cross-reactivity with other herpesviruses or adenoviruses. CMV infection is usually seen in immunocompromised patients and involves the GI tract, lung, heart, and liver, among other organs.
Antibody Isotype:
IgG2a-k, IgG1-k
Monosan Range:
MONOSAN
Clone:
DDG9/CCH2
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Plachter B et al. Virus Research 1992;24:265-76
References 2:
Evans PC et al. J Hepatol. 1999 Nov;31(5):913-20
References 3:
Pecorell I et al. Br J Opthalmol. 2000 Nov;84(11):1275-81
Anti-Cytokeratin (OSCAR) is well suited to distinguish epithelial carcino¬ma from non-epithelial malignancies and is used to aid epithelial tumor classification. This antibody has been used to characterize the source of various neoplasms and to study the distribution of keratin containing cells in epithelia during normal development and during the develop¬ment of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in recognizing epithelial cells, and carcinomas.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
OSCAR
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Gown, AM, et al. Am J Clin Pathol 1985;84:413
References 2:
Battifora, H. Am J Surg Pathol 1988;12:24
References 3:
Lewis JE et al. Hum Pathol. 1997 Jun;28(6):664-73
References 4:
Mueller JD et al. Cancer. 2000 Nov 1;89(9):1874-82
The antibody is the broad-spectrum keratin antibody cocktail. It is composed of mouse monoclonal antibody AE1 that recognizes the acidic type I keratins 10, 14, 15, 16, 19, and AE3 that reacts with the basic type II keratins 1, 2, 3, 4, 5, 6, 7, and 8. Both clones were generated using epidermal keratin as immunogen. This antibody detects carcinomas of different organ origin, but is most frequently negative in hepatocellular carcinoma, chromophobe RCC, adrenol cortical carcinoma, some clear cell renal cell carcinomas, and renal oncocytoma. This antibody cocktail can cross-react with other intermediate filaments, such as glial fibrillary acidic protein, giving a false-positive staining in glial tumors.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN
Clone:
AE1/AE3
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
The antibody is an antibody to high molecular weight cytokeratin that reacts with all squamous and ductal epithelium and stains carcinomas. This antibody recognizes cytokeratins 1,5,10, and 14 that are found in complex epithelia. Anti-Cytokeratin, 34betaE12 shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands; there has been no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, and neural tumors are unreactive with this antibody with some exceptions. Anti-Cytokeratin, 34betaE12 does label myoepithelial cells and has been shown to be useful in distinguishing prostatic adenocarcinoma from hyperplasia of the prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN
Clone:
34betaE12
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Gown AM, et al. Am J Pathol. 1984; 114:309
References 2:
OMalley FP, et al. Virch Arch A. 1990; 417:191-6
References 3:
Wojno KJ, et al. Am J Surg Pathol. 1995; 19:251-60
References 4:
Moinfar F, et al. Am J Surg Pathol. 1999; 23:1048-58
Cytokeratins 8 &18 (CK 8 & 18) are expressed in most simple epithelia (e.g. thyroid, breast, gastrointestinal tract, and respiratory tract). Anti-CK 8 & 18 have been reported to stain most adenocarcinomas and squamous cell carcinomas, but not some well-differentiated squamous cell carcinomas. Cytokeratin 8 & 18 have been reported to be useful markers for identifying Paget cells, colorectal carcinoma metastases,5 and gastric cancer micro metastases.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
B22.1&B23.1
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Angus B, et al. J Pathol. 1987; 155:377-84
References 2:
Corson, JM. Pathol Annu. 1986; 21:47-81
References 3:
Moll R, et al. Histochem Cell Biol. 2008; 129:705-33
Cytokeratin 8, a member of the Type II family of cytokeratins, is typically expressed in simple epithelium. The dimerization of cytokeratin 8 with cytokeratin 18 (labeled by 35betaH11) in the cytoplasm of simple epithelial cells allows for the formation of an intermediate filament cytoskeletal framework. This structure plays a role in the maintenance of cellular structural integrity and also functions in promoting signal transduction and cellular differentiation processes. Additionally, the presence of cytokeratin 8 has been detected in neoplastic epithelia, including glandular epithelium that can be found in prostate carcinoma. Positive immunoreactivity with anti-cytokeratin 8 is a useful indicator for the identification of normal and neoplastic epithelial tissues.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
35betaH11
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Battifora, H. Am J Surg Pathol 1988;12:24
References 2:
Gown, AM, et al. Am J Clin Pathol 1985;84:413
References 3:
Ljung G, et al. Prostate. 1997; 31:91-7
References 4:
Murata T, et al. Pathol Res Pract. 1993; 189:888-93
References 5:
Moll R, et al. Histochem Cell Biol. 2008; 129:705-33
Anti-CK 5/6 positivity is seen in nearly 100% of malignant mesotheliomas and in nearly 0% of lung adenocarcinomas. Anti-CK 5/6 positivity can be seen in undifferentiated large cell carcinoma as well as squamous carcinoma, and has been useful in recognizing spindle cell squamous cell carcinoma of the skin. Less than 10% of carcinomas of the breast, colon, and prostate stain positively for this marker. Anti-CK 5/6 has also been used successfully as a myoepithelial cell marker in the prostate and breast to determine malignancy.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
D5/16B4
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Ordonez NG. Am J Surg Pathol 1998; 22(10):1215-1221
References 2:
Abarahams NA et al. Am J Clin Pathol. 2003 Sep;120(3):368-76
References 3:
Reis-Filho JS et al. Virchows Arch. 2003 Aug;443(2):122-32
References 4:
Lin L et al. J Cutan Pathol.2003 Feb;30(2):114-7
References 5:
Otterbach F et al. Histopathology. 2000 Sep;3793):232-40
Cytokeratin 5 is an intermediate filament protein of 58 kD molecular weight within the cytokeratin family. It is a type II (basic) cytokeratin. Antibodies to this protein identify basal cells of squamous and glandular epithelia, myoepithelia, and mesothelium. Anti-cytokeratin 5 has been reported useful in the differential diagnosis of metastatic carcinoma in the pleura versus epithelioid mesothelioma. Epithelioid mesotheliomas are strongly positive in almost all cases, but a minority of pulmonary adenocarcinomas will show focal immunoreactivity. Almost all squamous cell carcinomas, half of transitional carcinomas, and many undifferentiated large cell carcinomas immunostain with anti-CK 5. Anti-CK 5, along with antip63, affords a high sensitivity and specificity for squamous differentiation. Myoepithelial cells of the breast, glandular epithelia, and basal cells of the prostate are labeled with anti-CK. This antibody, along with anti-CK 14, has found application in identifying basal-like breast carcinoma.
Antibody Isotype:
IgG
Monosan Range:
MONOSAN
Clone:
EP1601Y
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Ordonez NG. Human Pathology. 2007; 38:116
References 2:
Kargi A, et al. Appl Immunohistochem Mol Morphol.; 15:415420 (2007)
Anti-Cytokeratin 19 reacts with a wide variety of epithelium and epithelial malignancies including Adenocarcinomas of the Colon, stomach, pancreas, biliary tract, liver and breast. Perhaps the most useful application is the identification of Thyroid carcinoma of the Papillary type, although Follicular Carcinoma is also labeled by this antibody approximately 50-60% of the time.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
A53-B/A2.26
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Cerilli LA, et al. Am J Clin Pathol 2002;118:186-193
References 2:
Jain R, et al. Appl Immunohistochem Mol Morphol. 2010; 18:9-15
Cytokeratin 14 is a member of the Type I family of cytokeratins and is generally expressed in the basal cell layer of squamous epithelium. The cytokeratin 14 protein forms a heterotetramer with homodimers of cytokeratin 5 to contribute to the structural integrity of the intracellular cytoskeletal network. Anticytokeratin 14 has immunohistochemical utility as an aid in distinguishing squamous cell carcinomas from other tumors of epithelial origin.
Antibody Isotype:
IgG3
Monosan Range:
MONOSAN
Clone:
LL002
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Reis-Filho JS et al. Appl Immunohistochem Mol Morphol; 11(1):1-8 (2003)
Cyclins are proteins that govern transitions through distinct phases of the cell cycle by regulating the activity of the cyclin-dependent kinases. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene found in a wide variety of human neoplasms. This antibody is useful for distinguishing mantle cell lymphomas (cyclin D1 positive).
Antibody Isotype:
IgG
Monosan Range:
MONOSAN
Clone:
SP4
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Aagaard, L, et al. International J of Cancer 1995;61(1):115-120
References 2:
Bartkova, J, et al. Cancer Research 1995;55:949-956
References 3:
Bartkova, J, et al. Oncogene 199510(4):775-778
References 4:
Bartkova, J, et al. J of Pathology 1994;172(3):237-245
References 5:
Lukas, J, et al. Molecular and Cellular Biology 1995;15(5):2600-2611
Collagen Type IV is the major component of the basal lamina so antibodies to this molecule confirm its presence and reveal the morphological appearance of the structure. Normal tissue stains with this antibody in a fashion consistent with the sites of mesenchymal elements and epithelial basal laminae. Anti-Collagen IV can also be useful in the classification of soft tissue tumors; schwannomas, leiomyomas. Their well differentiated, malignant counterparts usually immunoreact with this antibody. The vascular nature of neoplasms, hemangiopericytoma, angiosarcoma and epithelioid hemangioendothelioma can be revealed by this antibody with greater reliability than non-specific stains (e.g. silver reticulum).
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN
Clone:
CIV22
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Gould, VE, et al., Pathol Annul 1976;11:353-386
References 2:
McArdle, JP, et al., Int J Cancer 1984;34:633-638
References 3:
De Iorio P et al. Anticancer Res. 2001;21(2B):1395-9
References 4:
Maatta M et al. J Histochem Cytochem. 2001 Jun;49(6):711-26
References 5:
Schmehl K et al. Int J Colorectal Dis. 2000 Feb;15(1):39-48
Anti-CEA is employed as a tool to assist in the distinction between adenocarcinoma and mesotheliomas, along with other markers such as calretinin, CK 5/6, D2-40, HBME-1, Napsin A, MOC31, and Ber-EP4. Anti-CEA positivity is seen in adenocarcinomas from the lung and colon, as well.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
CEA31
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Go, VLW, et al., Cancer 1976;37:562-566
References 2:
Delellis, RA, et al., Am J Clin Pathol 1978;50:587-594
References 3:
Abutaily AS et al. J Clin Pathol. 2002 Sep;55(9):662-8
References 4:
Bhatnagar J et al. Anticancer Res. 2002;22(3):1849-57
References 5:
Carella R. et al. Am J Surg Pathol. 2001 Jan;25(1):43-50
CDX-2 is a caudal-related homeobox transcription factor whose expression in the adult is normally present in the gastrointestinal (GI) epithelium. It is implicated in the development and maintenance of the intestinal mucosa. This protein is expressed immunohistochemically in the nuclei of normal GI epithelium. CDX-2 protein expression has been seen in GI carcinomas. Anti-CDX-2 has been useful to establish GI origin of metastatic adenocarcinomas and carcinoids and is especially useful to distinguish metastatic colorectal adenocarcinoma from lung adenocarcinoma. However, mucinous carcinomas of the ovary also stain positively with this antibody, which limits the usefulness of this marker in the distinction of metastatic colorectal adenocarcinoma versus mucinous carcinoma of the ovary.
Antibody Isotype:
IgG
Monosan Range:
MONOSAN
Clone:
EPR2764Y
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Mazziotta RM, et al. Appl Immunohistochem Mol Morphol. 2005; 13:55-60
References 2:
Erickson LA, et al. Endocr Pathol. 2004; 15:247-52
References 3:
Saqi A, et al. Am J Clin Pathol. 2005; 123:394-404
References 4:
Saad RS, et al. Am J Clin Pathol. 2004; 122:421-7
References 5:
Kaimaktchiev V, et al. Mod Pathol. 2004; 17:1392-9
CD99, as detected with a variety of antibodies, is expressed by virtually almost all Ewings sarcoma and primitive peripheral neuroectodermal tumors (ES/PNET) and demonstrates strong and diffuse membranous staining. Other tumors that may show CD99 expression include neuroendocrine carcinomas, mesenchymal chondrosarcomas, solitary fibrous tumors, synovial sarcomas, vascular tumors, small round blue cell tumors, lymphoblastic lymphoma, acute myeloid leukemia, and myeloid sarcoma.5 However, strong and diffuse membranous reactivity for CD99 favors ES/PNET over the other diagnostic considerations. The other CD99+ tumors usually show cytoplasmic and more heterogeneous staining. Therefore, when making a final diagnostic interpretation, CD99 must be considered in a panel with other antibodies.
Antibody Isotype:
IgG
Monosan Range:
MONOSAN
Clone:
EPR3097Y
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Rettig WJ, et al. Lab Invest. 1992; 66:133
References 2:
Fellinger EJ, et al. Amer J Surg Pathol. 1992; 16:746
References 3:
Ambros IM, et al. Cancer. 1991; 139:317
References 4:
Khoury JD. Adv Anat Pathol. 2005; 12:212-20
References 5:
Dabbs DJ. Theranostic and Genomic Applications. 2014; 126
The CD8 (cluster of differentiation 8) antigen is a cell surface glycoprotein made up of two subunits alpha and beta.1 Anti-CD8 is a T-cell marker for the detection of cytotoxic/suppressor lymphocytes. CD8 is also detected on NK cells, some thymocytes, some null cells and bone marrow cells. This antibody, along with other markers, can be used to distinguish between reactive and neoplastic Tcells.3 CD8 expression has been found to be negative in Mycosis Fungoides. Rarely does anti-CD8 label non-hematolymphoid neoplasms.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN
Clone:
C8/144B
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Rossi, ML, Sanchez, FC, et al., J Clin Path 1988;41:314-319
References 2:
Stein, H, Lennart, K, et al., Adv Cancer Res 1984;42:67-147
References 3:
Phan-Dinh-Tuy, F, Niaudet, P, et al., Mol Immun 1982;19:1649-1654
The antibody is a B-cell marker that is generally used to complement CD20. This antibody will stain many of the same lymphomas as CD20, but also is more likely to stain precursor B-lymphoid leukemias than CD20. Anti-CD79a also stains more cases of plasma cell myeloma and occasionally some types of endothelial cells as well. Anti-CD79a will stain many cases of acute promyelocytic leukemia (FAB-M3), but only rarely stains other types of myeloid leukemia.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN
Clone:
JCB117
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Mason DY, et al., Eur J Immun; 22:2753-2756 (1992)
References 2:
Lin BT, Weiss LM. Hum Pathol.; 28(9):1083-90 (1997)
References 3:
Pilozzi E et al. J Pathol.; 186(2):140-3 (1998)
References 4:
Kurtin PJ et al. Am J Clin Pathol.; 112(3):319-29 (1999)
References 5:
Blakolmer K et al. Mod Pathol.; 13(70:766-72 (2000)
CD7 antigen is a 40-kDa cell surface glycoprotein that is a member of the immunoglobulin gene superfamily. While its precise function is not known, it is suggested that CD7 plays a role in T-cell interactions as it is one of the earliest T-cell lineage associated antigens expressed during T-cell ontogeny. CD7 is expressed in thymocytes, mature peripheral T-cells, natural killer cells, and lymphoid and myeloid progenitors. CD7 is the most consistently expressed T cell antigen in lymphoblastic lymphomas and leukemias, and is therefore a useful marker in the identification of such neoplastic proliferations. In mature post-thymic T cell neoplasms, it is the most common pan-T antigen to be aberrantly absent and its absence in a T cell population is a useful pointer to a neoplastic conversion.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN
Clone:
MRQ-56
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Hodak E, et al. J Am Acad Derma¬tol. 2006 Aug;55(2):276-84
References 2:
Stillwell R, et al. Immunol Res. 2001; 24:31-52
References 3:
Schanberg LE, et al. Proc Natl Acad Sci USA. 1991; 88:603-7
The antibody marks cells of monocyte/macrophage lineage. This antibody is capable of staining monocytes, Kupffer cells, osteoclasts, granulocytes and their precursors; lymphomas are negative or show few granules. This antibody may be useful for the identification of myelomonocytic and histiocytic tumors. Since this detects a formalin-resistant epitope that may be associated with lysosomal granules, other lysosome-rich cells may also stain.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN
Clone:
Kp-1
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
CD61 also known as integrin beta chain beta 3 (ITGB3) is an integrin cell-surface protein associated with cellular adhesion and cell-surface mediated signaling. Immunohistochemical staining for CD61 can be useful in evaluating normal and abnormal megakaryocytes, which can aide in the identification of some hematopoietic malignancies. Anti-CD61 reactivity is also seen in platelets, osteoclasts and macrophages.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN
Clone:
2f2
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Duperray A et al. Blood. 1989 Oct; 74(5):1603-11
References 2:
Goldman BI et al. Modern Pathology 14:589-594 (2001)
References 3:
Thiele J et al. Virchows Archiv B Cell Pathol (1990) 58:295-302
CD56, also known as neural cell adhesion molecule (NCAM), is a calcium-independent homophilic binding protein that belongs to a group of cell adhesion molecules including cadherins, selectins, and integrins. CD56 is involved in cellcell adhesion of neural cells during embryogenesis and is expressed on most neuroectodermally derived tissues. In normal tissue, anti-CD56 labels neurons, glia, schwann cells, NK (natural killer) cells, and a subset of T-cells.3 CD56 expression can be seen in most NK cell neoplasms, certain subtypes of T-cell lymphoma and in some plasma cell neoplasms. well
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN
Clone:
123C3.D5
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Langdon, SP, et al. Cancer Research 1988;48(21):6161-6165
References 2:
Moolenaar, CE, et al. Cancer Research 1990;50(4):1102-1106
References 3:
Sumi M et al. Leuk Lymphoma. 2003 Jan; 44(1): 201-4
References 4:
Kibbelaar, RE, et al. Euro J of Cancer 1991;27(4):431-435
References 5:
Michalides, R, et al. International J of Cancer Sup 1994;8:34-37
Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells. CD5 expression is useful in distinguishing mature T-cell neoplasms and differentiating among mature small lymphoid cell malignancies. Anti-CD5 does not react with granulocytes or monocytes.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
SP19
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Jones NH, et al., Nature 1986;323: 346-349
References 2:
Tan SH et al. Br J Dermatol. 2003 Sep;149(3): 542-53
References 3:
Chang CC et al. Mod Pathol. 2002 Oct;15(10): 1051-7
References 4:
Hatano B et al. Pathol Int. 2002 May-Jun;52(5-6): 400-5
References 5:
West RB et al. Am J Clin Pathol. 2002 Apr;117(4): 636-43
Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells. CD5 expression is useful in distinguishing mature T-cell neoplasms and differentiating among mature small lymphoid cell malignancies. Anti-CD5 does not react with granulocytes or monocytes.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN
Clone:
4C7
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Jones NH, et al., Nature 1986;323: 346-349
References 2:
Tan SH et al. Br J Dermatol. 2003 Sep;149(3): 542-53
References 3:
Chang CC et al. Mod Pathol. 2002 Oct;15(10): 1051-7
References 4:
Hatano B et al. Pathol Int. 2002 May-Jun;52(5-6): 400-5
References 5:
West RB et al. Am J Clin Pathol. 2002 Apr;117(4): 636-43
Anti-CD45 (anti-leukocyte common antigen) is routinely used to aid the differential diagnosis of undifferentiated neoplasms, whenever malignant lymphoma is suspected by the morphological or clinical data. It is a highly specific antibody; therefore a positive result is highly indicative of hematolymphoid origin. Certain types of hematolymphoid neoplasms may lack CD45 (Hodgkin lymphoma, some T-cell lymphomas, and some leukemias) so its absence does not rule out a hematolymphoid tumor. This antibody is expressed almost exclusively by cells of hematopoietic lineage and is present in most benign and malignant lymphocytes as well as plasma cell precursors.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN
Clone:
2B11 & PD7/26
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Mason, DY, Am Pathol 1987;128:1-4
References 2:
Hall PA, Histopathology 1988;13:149-160
References 3:
Kurtin, PJ, Hum Path 1985;16:353-365
References 4:
Maluf HM et al. Mod Pathol. 1995 Feb; 8(2): 155-9
References 5:
Caballero T et al. J Clin Pathol. 1995 Aug;48(8): 743-8
The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95 kD or hematopoietic variant (CD44s) that is found in mesodermal cells such as hematopoietic, fibroblastic, and glial cells, as well as in some carcinoma cell lines. Higher molecular weight isoforms have been described in epithelial cells (CD44v) and are thought to function in intercellular adhesion and stromal binding. While many human tumors express CD44, a positive correlation between CD44v expression and tumor dedifferentiation has been demonstrated. MON 3237 may be useful in discrimination of urothelial carcinoma in-situ from non-neoplastic changes in the urothelium.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
MRQ-13
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Hudson D, et al. Int. J. Cancer. 1996; 66:457-63
References 2:
East JA, et al. Eur J Cancer. 1993; 29A:1921-2
References 3:
Gadalla HA, et al. BJU Int. 2004; 93:151-5
References 4:
McKenney JK, et al. Am J Surg Pathol. 2001; 25:1074-8
References 5:
Lopez-Beltran A, et al. Anal Quant Cytopathol Histpathol. 2013; 35:121-9
CD31 has cytoplasmic, membranous expression in non-neoplastic and neoplastic vascular endothelial cells.1 It has been used as a tool to identify the vascular origin of neoplasms such as angiosarcomas, Kaposi sarcomas and epithelioid hemangioendothelioma. Immunohistochemical study with CD31 has also been shown useful to detect areas of tumor lymphovascular invasion. Additionally, detection of weak diffuse cytoplasmic CD31 immunoreactivity has been seen in cases of various carcinomas with occasional membranous staining in ductal carcinomas of the breast as well as in intratumoral macrophages.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN
Clone:
JC70
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Parums DV, et al.. J Clin Pathol. 1990; 43:752-7
References 2:
Attanoos RL, et al. Thorax. 2000; 55:860-3
References 3:
Alexander-Sefre F, et al. J Clin Pathol. 2003; 56:786-8
The antibody detects a formalin-resistant epitope that is expressed by Reed-Sternberg cells in classic Hodgkin lymphoma, the majority of anaplastic large cell lymphomas, primary cutaneous CD30 positive T-cell lymphoproliferative disorders and in embryonal carcinomas. Occasionally diffuse large B-cell lymphoma stains with this antibody. This antibody also stains plasma cells in paraffin-embedded tissue as well as reactive immunoblasts. The staining pattern of anti-CD30 in lymphoma and embryonal carcinoma is different, with the former being membranous and exhibiting Golgi zone accentuation in location, and the latter being membranous only.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN
Clone:
Ber-H2
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Schwarting R, et al., Blood 1989 (74):1678-1689
References 2:
Fonatsch C, et al., Genomics 1992 (14):825-826
References 3:
George DH et al. Am J Surg Pathol. 2003 Apr;27(4): 487-93
References 4:
Hedvat CV et al. Hum Pathol. 2002 Oct;33(10): 968-74
CD3s immunohistochemical detection locates the cytoplasmic component of CD3 protein. Anti-CD3 is considered to be a pan-T-cell marker and reacts with an antigen present at the surface and in the cytoplasm of T lymphocytes. Anti-CD3 is widely used for the identification of immature and mature T-cell malignancies.
Antibody Isotype:
IgG
Monosan Range:
MONOSAN
Clone:
MRQ-39
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Beverley PC, et al. Eur J Immunol.; 11:329-34 (1981)
References 2:
Hedvat CV, et al. Hum Pathol.; 33:968-74 (2002)
References 3:
Karube K, et al. Am J Surg Pathol.; 27:1366-74 (2003)
References 4:
Dogan A, et al. Am J Surg Pathol.; 27:903-11 (2003)
CD25, Interleukin-2 receptor alpha chain, is the alpha subunit of the cell surface receptor which regulates regulatory T-cells. CD25 has been detected in various hematological malignancies including adult T-cell leukemia/lymphoma, and hairy cell leukemia. MON 3230 has also been useful in identifying mast cells in skin biopsies in the setting of Urticaria Pigmentosa, which is predictive of Systemic Mastocytosis.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
4C9
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Hahn HP, et al. Am J Surg Pathol. 2007 Nov;31(11):1669-76
References 2:
Hollmann TJ, et al. Am J Surg Pathol. 2008 Jan;32(1):139-45
References 3:
Létourneau S, et al. Clin Immunol. 2009; 123:758-62
References 4:
De Totero D, et al.. Leuk Lymphoma. 1994; 104:412-9
References 5:
Qayyum S, et al. Archives of Pathology & Lab Med. 2014; 138:282-6
CD23 antigen is a 45-60 kDa membrane glycoprotein identified as a low affinity receptor for IgE production as well as a receptor for lymphocyte growth factor. CD23 is found in some mature B-cell lymphomas and in Reed-Sternberg cells in Hodgkin disease. Follicular dendritic cells and some activated B-cells within germinal centers express CD23 in high density and mantle zone B-cells are stained. The majority of chronic lymphocytic leukemias/small lymphocytic lymphomas are anti-CD23 positive, whereas mantle cell lymphomas are generally negative, so this marker is useful when applied with other markers to separate the small cell lymphomas.1,3 Precursor B and T lymphomas, myeloid neoplasms, and mature T-cell lymphomas are CD23 negative and other small cell lymphomas are occasionally positive. Anti-CD23 is expressed in activated mature B-cells expressing IgM or IgD, monocytes/macrophages, follicular dendritic cells, T-cell subsets, eosinophils, Langerhans cells and small lymphocytic lymphoma/chronic lymphocytic leukemia.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN
Clone:
1B12
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Kaiserlian, D, et al., Immunology 1993;80:90-95
References 2:
Aubry, JP et al., Oxford Univ Press- Oxford, NY, Tokyo 1987:417-419
References 3:
Pallesen G, Oxford Univ Press-Oxford, NY, Tokyo 1987:383-386
References 4:
Pezzella F et al. Br j Haematol. 2000 Feb;108(2): 369-76
References 5:
Kurtin PJ et al. Am J Clin Pathol. 1999 Sep;112(3): 319-29
CD21 (also known as complement receptor 2 (CR2), C3d receptor, or EBV receptor) is a 140 kDa membrane protein on B-lymphocytes to which the Epstein-Barr virus (EBV) binds during infection of these cells. The antigen is absent on T-lymphocytes, monocytes, and granulocytes. MON 3028 is useful in the identification of follicular dendritic cell matrix found in normal lymph node and tonsillar tissue. This antibody also labels follicular dendritic cell sarcomas. Anti-CD21 is valuable in differentiating follicular lymphoma with marginal zone differentiation from marginal zone lymphoma with follicular involvement. It also plays a role in distinguishing among nodular lymphocyte predominant Hodgkin lymphoma, lymphocyte-rich classic Hodgkin lymphoma, and T-cell/histiocyte-rich B-cell lymphoma in combination with other B-cell and T-cell markers. Anti-CD21 is also useful in identifying abnormal follicular dendritic cell pattern in angioimmunoblastic T-cell lymphoma and follicular T-cell lymphoma.
Antibody Isotype:
IgG
Monosan Range:
MONOSAN
Clone:
EP3093
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Dillon KM et al. J Clin Pathol. 2002 Oct;55(10):791-4
CD21 (also known as complement receptor 2 (CR2), C3d receptor, or EBV receptor) is a 140 kDa membrane protein on B-lymphocytes to which the Epstein-Barr virus (EBV) binds during infection of these cells. The antigen is absent on T-lymphocytes, monocytes, and granulocytes. MON 3027 is useful in the identification of follicular dendritic cell matrix found in normal lymph node and tonsillar tissue. This antibody also labels follicular dendritic cell sarcomas. Anti-CD21 is valuable in differentiating follicular lymphoma with marginal zone differentiation from marginal zone lymphoma with follicular involvement. It also plays a role in distinguishing among nodular lymphocyte predominant Hodgkin lymphoma, lymphocyte-rich classic Hodgkin lymphoma, and T-cell/histiocyte-rich B-cell lymphoma in combination with other B-cell and T-cell markers.6 Anti-CD21 is also useful in identifying abnormal follicular dendritic cell pattern in angioimmunoblastic T-cell lymphoma and follicular T-cell lymphoma.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
2G9
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Dillon KM et al. J Clin Pathol.; 55(10):791-4 (2002)
References 2:
Cheuk W, et al. Am J Surg Pathol.; 25:721-31 (2001)
References 3:
Pileri SA, et al.Histopathology.; 41:1-29 (2002)
References 4:
Maeda K, et al. J Histochem Cytochem.; 50:1475-1486 (2002)
CD20 is a transmembrane protein in late B-cell precursors and mature B-cells that plays a role in regulating proliferation and differentiation. CD20 expression is lost at the plasma cell stage of differentiation. MON 3226 (pan B-cell) has rarely been detected in T-cell malignancies, and is a dependable marker of B-cell lymphomas such as DLBCL. CD20 expression is present in some thymomas. It does not cross-react with non-hematopoietic neoplasms.
Antibody Isotype:
IgG2a-k
Monosan Range:
MONOSAN
Clone:
L26
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
CD2 is a surface antigen present on all peripheral T-cell lymphocytes. CD2 is associated with signaling and mediating adhesion to other T-cells through the lymphocyte function-associated antigen and CD48/BCM1 complex. MON 3225 is a useful immunohistochemical reagent for identification of normal T-lymphocytes, and for assessing lymphoid malignancies.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN
Clone:
MRQ-11
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Aguilera NS, et al. Arch Pathol Lab Med. 2006; 130:1772-9
References 2:
Barrionuevo C, et al. Appl Immunohistochem Mol Morphol. 2007; 15:38-44
References 3:
Bovenschen HJ, et al. Br J Dermatol. 2005; 153:72-8
References 4:
Foon KA, et al. Blood. 1986; 68:1-31
References 5:
Gonzalez L, et al. Journal of Comparative Pathology 2001; 125:41-7
CD19 is a glycoprotein on the surface of mature B cells, it works in conjunction with receptors and proteins to regulate B-cell signaling. CD19 is present in both normal and malignant B cells, and hence being valuable for the identification of B-cell neoplasms such as diffuse large B-cell lymphoma.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN
Clone:
MRQ-36
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Steinmetz OM, et al. Transplantation. 2007 15;84(7):842-50
References 2:
Teng YK, et al. Arthritis Rheum. 2007 ;56(12):3909-18
CD163, also known as scavenger receptor cysteine-rich type 1 protein M130, is an acute phase-regulated and signal-inducing transmembrane protein, found exclusively on cells of monocytic origin. CD163 plays a critical role in macrophage clearance and endocytosis of hemoglobin/haptoglobin complexes. Therefore, CD163 contributes to the anti-inflammatory response and protects tissues from oxidative and inflammatory hemoglobin. Anti-CD163 labels cells of monocytic-macrophage lineage, with expression in bone marrow3 and histiocytic neoplasms. Solubilized in plasma, CD163 functions as an anti-inflammatory signal and has many roles in disease processes that range from autoimmune conditions such as rheumatoid arthritis to atherosclerosis.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MRQ-26
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Buechler C, et al. J Leukoc Biol. 67:97-103 (2000)
References 2:
Kristiansen M, et al. Nature. 409:198-201 (2001)
References 3:
Etzerodt A. et al. Antioxid Redox Signal. 18: 2352-63 (2013)
CD15 is a carbohydrate antigen with the common trisaccharide structure 3-fucosyl-N-acetyllactosamine, also known as Lewis x (Lex) or stage-specific embryonic antigen 1 (SSEA-1).1-3 CD15 is expressed in myeloid cells and mediates neutrophil adhesion to dendritic cells.2-3 CD15 is also expressed in Reed-Sternberg cells and is thus a useful marker for identifying Hodgkins lymphoma.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
MMA
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Pellegrini W, et al. Haematologica. 2007; 92:708-9
CD14 is a 55kDa glycosyl-phosphatidylinositol-linked membrane protein, involved in endotoxin binding and recognition of apoptotic cells. CD14 is expressed on monocytes, macrophages, follicular dendritic cells, and granulocytes. Anti-CD14 can detect these cells, including monocyte-derived cells which are frequently increased in diffuse large B-cell lymphoma (DLBCL), as well as in neoplastic cells in acute myeloid leukemia with monocytic differentiation and chronic myelomonocytic leukemia.
Antibody Isotype:
IgG
Monosan Range:
MONOSAN
Clone:
EPR3653
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Gregory CD, et al. Apoptosis. 1999; 4:11-20
References 2:
Wright SD, et al. Science. 1990; 249: 1431-33
References 3:
Marmey B, et al. Hum Pathol. 2006; 37:68-77
References 4:
Smeltzer JP, et al. Clin Cancer Res. 2014; 20:2862-72
References 5:
Rollins-Raval MA, et al. Histopathology. 2012; 60: 933-42
CD138, Syndecan 1, is expressed in the late stages of B-cell differentiation with progression towards plasma cells. It can be used to differentiate lymphoplasmacytic lymphoma from marginal zone lymphoma. ALK+ large B-cell lyphoma (LBCL) usually strongly expresses CD138 whereas lineageassociated markers such as anti-CD20 and anti-CD79a do not stain ALK+LBCL. Anti-CD138 is immunoreactive with HHV8-associated primary effusion lymphoma even though the lymphoma cells lack the expression of B-cell markers. Anti-CD138 is a good marker to identify and enumerate plasma cells, benign, reactive, or malignant, in bone marrow biopsy specimens.4,6 CD138 is also expressed in epithelial cells.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
B-A38
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Chilosi M, et al. Mod Pathol. 1999; 12:1101-6
References 2:
Sebestyén A, et al. Br J Haematol. 1999; 104:412-9
References 3:
Bayer-Garner IB, et al. Mod Pathol. 2001; 14:1052-8
References 4:
OConnell FP, et al. Am J Clin Pathol. 2004; 121:254-63.
References 5:
Colomo L, et al. Am J Surg Pathol. 2004; 28:736-47
CD117, c-kit is a tyrosine kinase receptor found on interstitial cells of Cajal, germ cells, bone marrow stem cells, melanocytes, breast epithelium and mast cells. This receptor is found on a wide variety of tumor cells (follicular and papillary carcinoma of thyroid, adenocarcinomas from endometrium, lung, ovary, pancreas, breast; malignant melanoma, endodermal sinus tumor, and small cell carcinoma) but has been particularly useful in differentiating gastrointestinal stromal tumors from Kaposis sarcoma, and tumors of smooth muscle origin.
Antibody Isotype:
IgG
Monosan Range:
MONOSAN
Clone:
YR145
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Sircar K, et al. AM J Surg Pathol 23(4):377-389,1999
References 2:
Miettinen M et al. Am J Surg Pathol 24(2):211-222, 2000
References 3:
Miettinen M. et al. Am J Surg Pathol 23(9): 1109-1118
Common acute lymphoblastic leukemia antigen (CALLA / CD10) is a useful marker for the characterization of childhood leukemia and B cell lymphomas. This antibody reacts with antigen of lymphoblastic, Burkitts, and follicular lymphomas; and chronic myelocytic leukemia. Also, Anti-CD10 detects the antigen of glomerular epithelial cells and the brush border of the proximal tubules; this characteristic may be helpful in interpreting renal ontogenesis in conjunction with other markers. Other non-lymphoid cells that are reactive with CD10 are breast myoepithelial cells, bile canaliculi, neutrophils and small population of bone marrow cells, fetal small intestine epithelium, and normal fibroblasts.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
56C6
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Maguer-Satta V, et al.. Stem Cells. 2011; 29:389-96
Calponin is a 34 kD polypeptide that interacts with actin, tropomyosin, and calmodulin. It is involved in smooth muscle contraction mechanism and is restricted exclusively to smooth muscle tissue. Anticalponin has been found to be useful in staining myoepithelium and is, therefore, useful for differentiating benign sclerosing adenosis of the breast from infiltrating ductal carcinoma. Calponin positivity has also been noted in malignant myoepithelioma and pleomorphic adenoma3 of salivary gland origin, as well as angiomatoid malignant fibrous histiocytoma.
Antibody Isotype:
IgG
Monosan Range:
MONOSAN
Clone:
EP798Y
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Wang NP, et al. Appl Immunohistochem. 1997; 5:141-151
References 2:
Nagao T, et al. Cancer. 1998; 83:1292-9
References 3:
Savara AT, et al. Mod Pathol. 1997; 10:1093-1100
References 4:
Fanburg-Smith JC, et al. Hum Pathol. 1999; 30:1336-43
References 5:
Hornick JL, et al. Am J Surg Pathol. 2003; 27: 1183-96
Calponin is a 34-kD actin filament-associated regulatory protein that interacts with actin, tropomyosin, and calmodulin. It is involved in the smooth muscle contraction mechanism and is restricted exclusively to smooth muscle tissue and myoepithelial cells. Anti-calponin has been found to be useful marker for differentiating benign sclerosing lesions of the breast from invasive carcinoma.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN
Clone:
CALP
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Anti-caldesmon is a regulatory protein found in smooth muscle and other tissues which interacts with actin, myosin, tropomyosin, and calmodulin. Anti-caldesmon antibody labels smooth muscle and tumors of smooth muscle, myofibroblastic, and myoepithelial differentiation. Anti-caldesmon has also been used to differentiate epithelioid mesothelioma from serous papillary carcinoma of the ovary.
Antibody Isotype:
IgG
Monosan Range:
MONOSAN
Clone:
E-89
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Miettinen M, et al. Arch Pathol Lab Med. 2006; 130:1466-78
References 2:
Watanabe K, et al. Hum Pathol. 1999; 30:392-6
References 3:
McCluggage WC. Adv Anat Pathol. 2004; 11:162-71
References 4:
Comin CE, et al. Am J Surg Pathol. 2006; 30:463-9
References 5:
Comin CE, et al. Am J Surg Pathol. 2007; 31:1139-48
The antibody reacts with epithelioid malignancies of the ovary, papillary serous carcinoma of the cervix, adenocarcinoma of the endometrium, clear cell adenocarcinoma of the bladder, and epithelioid mesothelioma. The antigen is formalin resistant, permitting the detection of ovarian cancer by immunohistochemistry, although serum assays for this protein are widely used to monitor ovarian cancer. MON 3211 also reacts with antigens in seminal vesicle carcinoma.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
OC125
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Kabawat S, et al. Int J Gynecol Pathol. 1983; 2:275-285
References 2:
Davis H, et al. Cancer Res. 1986; 46:6143-6148
References 3:
Zhou C, et al. Am J Surg Pathol. 1998; 22:113- 20
References 4:
Mylonas I, et al. Anticancer Res. 2003; 23:1075-80
References 5:
Fukazawa I, et al. Arch Gynecol Obstet. 1988; 243:41-50
Beta-catenin is a 92 kD protein normally found in the cytoplasm of the cell in the submembranous location. Mutations in the beta-catenin gene result in nuclear accumulation of this protein. Nuclear accumulation of this protein has been demonstrated in fibromatosis (desmoid tumors) of the breast and abdomen and, therefore, is useful in differentiating from other spindle cell neoplasms that may occur in these locations.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
14
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Alman BA, et al. Am J Pathol. 1997; 151:329-34
References 2:
Li C, et al. Am J Pathol. 1998; 153:709-14
References 3:
Abraham SC, et al. Hum Pathol. 2002; 33:39-46
References 4:
Montgomery E, et al. Am J Surg Pathol. 2002; 26:1296-301
BCL6 is a transcriptional regulator gene which codes for a 706-amino-acid nuclear zinc finger protein. In normal tissue these antibodies have strong nuclear staining for a subset of B-lymphocytes, mostly located in germinal centers (GC). BCL6 antibodies stain malignant cells in follicular lymphoma, diffuse large B-cell lymphomas, Burkitt lymphoma,4 classical Hodgkin lymphoma, as well as majority of tumor cells in nodular lymphocyte predominant Hodgkin lymphoma. BCL6 expression has been also seen in anaplastic large cell lymphomas (ALCL)
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
GI191E/A8
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
BCL2 is a protein associated with apoptosis regulation produced by the bcl-2 gene, located on chromosome 14q32.BCL2 is comprised of an alpha (239 amino acids) and beta chain. BCL2 (and thus BCL2 alpha chain) is found in mitochondrial and nuclear membranes and in the cytosol rather than the cell surface. In normal lymphoid tissue, BCL2 antibody reacts with small B-lymphocytes in the mantle zone and many cells within the T-cell areas. Anti-BCL2 has shown consistent negative reaction on reactive germinal center B-cells and positive staining of neoplastic follicles in follicular lymphoma. This antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. This antibody may also be used in distinguishing between those follicular lymphomas that express BCL2 protein and the small number in which the neoplastic cells are BCL2 negative.
Antibody Isotype:
IgG
Monosan Range:
MONOSAN
Clone:
E17
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Cooper K, et al. Journal of Pathology. 1997; 182:307-10
References 2:
Chetty R, et al. J Clin Pathol. 1995; 48:1035-1038
Bcl-2 is the best characterized protein family involved in regulation of apoptotic cell death, consisting of anti-apoptotic and pro-apoptotic members. Bcl-2 is a useful marker for identifying neoplastic cells in follicular lymphoma. Antibodies specific for the Bcl-2 protein can be used to distinguish between reactive and neoplastic follicular proliferation in lymph node biopsies.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN
Clone:
124
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Tsujimoto, Y. Genes Cells. 1998; 3:697-707
References 2:
Gaulard P, et al. Am J Pathol. 1992; 140: 1089-95
References 3:
Wang T, et al. APMIS. 1995; 103:655-62
References 4:
West RB, et al. Am J Clin Pathol. 2002; 117:636-43
The antibody recognizes a human breast carcinoma associated glycoprotein BCA-225 (220-225kD). This protein differs in size and distribution from other breast carcinoma antigens. Anti-BCA-225 primary antibody labels breast cancer antigen 225 (BCA-225) in primary and metastatic breast carcinoma. BCA-225 was first identified in T47D breast carcinoma cells, but its expression in other carcinomas such as lung, kidney, ovary and endometrium has
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
Cu-18
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Mesa-Tejada R, et al. Am J Pathol; 1988 130:305-14
Annexin A1, also known as lipocortin I, is a protein that is encoded by the ANXA1 gene in humans. Annexin A1 is a useful marker for identifying hairy cell leukemia cells. ANXA1 is strongly expressed on the cell membrane and occasionally in the cytoplasm of tumor cells in 97% of samples from patients with hairy cell leukemia, it is therefore a useful marker for identifying hairy cell leukemia cells. By contrast, B-cell lymphomas other than hairy cell leukemia, including typical splenic lymphoma with villous lymphocytes and patients with variant hairy cell leukemiaas defined by current morphologic, phenotypic, and clinical criteriaare ANXA1-negative. Additionally, aberrant expression of Annexin A1 has been reported in certain types of breast and gastric carcinomas.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MRQ-3
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Falini B, et al. Lancet.; 363:1869-70 (2004)
References 2:
Sobral-Leite M, et al. BMC Med.; 13:156 (2015)
References 3:
Cheng TY, et al. Cancer.; 118:5757-67 (2012)
References 4:
Sato Y, et al. Exp Ther Med.; 2:239-43 (2011)
References 5:
Wang KL, et al.. Clin Cancer Res.; 12:4598-604 (2006)
Anaplastic lymphoma kinase (ALK) is a novel receptor protein-tyrosine kinase. ALK can create a fusion protein with a nuclear protein gene called nucleophosmin (NPM) via the amino terminus of NPM and the catalytic domain of ALK. The product of this fusion protein is oncogenic.1 Studies have found this chromosomal translocation in most anaplastic large-cell non-Hodgkin's lymphomas, making ALK a good marker for anaplastic large cell lymphomas
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN
Clone:
ALK-1
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Smooth Muscle Actin is a part of the actin family of proteins which are highly conserved and form microfilaments. These filaments are one of the major components of the cytoskeleton. Anti-smooth muscle actin immunohistochemical reactivity is seen in smooth muscle cells, myofibroblasts and myoepithelial cells.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN
Clone:
1A4
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Cooke PH. A. J Cell Biol. 1976; 68:539-56
References 2:
Skalli O, et al. J Cell Biol. 1986; 103:2787-96
References 3:
Perez-Montiel MD, et al. Am J Dermatopathol. 2006; 28:105-11
The antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Muscle Specific Actin is a part of the actin family of proteins which are highly conserved, major components of the cytoskeleton. Anti-Muscle Specific Actin immunohistochemical reactivity is seen in skeletal, cardiac, and smooth muscle cells and can be seen in neoplasms with muscle differentiation such as leiomyomas and rhabdomyosarcomas. In contrast, antiMuscle Specific Actin reactivity is typically not seen in endothelial cells, connective tissues, carcinomas, melanomas, lymphomas and most nonmyogenic sarcomas
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN
Clone:
HHF35
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Gown AM, et al. Am J Pathol. 1986; 125:191
References 2:
Schmidt RA, et al. Am J Pathol. 1988; 131:19-28
References 3:
Azumi N, et al.Mod Pathol. 1988; 1:469-74
References 4:
Rangdaeng S, et al. Am J Clin Pathol. 1991; 96:32-45
The monoclonal antibody BM8 recognises a 125 kD extracellular macrophage membrane molecule, highly restricted to mature macrophage subpopulations residing in tissue. This murine F4/80 glycoprotein contains seven-transmembrane (TM7) regions, which anchor the protein in the cell membrane, and thereby shows similarity in this region to G-protein-coupled receptors. The F4/80 molecule shares overall structural homology to other members of the epidermal growth factor (EGF)-TM7 family. The antigen is detected on tissue fixed macrophages in all organs tested so far (spleen, lymph nodes, thymus, liver, skin). It is also present on Langerhans cells in the skin and Kupffer cells in the liver. It is absent on granulocytes, lymphocytes and trombocytes. The expression of F4/80 increases upon maturation of macrophage precursors in bone marrow and blood as well as in ontogeny.</br> The monoclonal antibody BM8 is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas. Furthermore it is a unique histological marker of the progression from peri-insulitis to beta-cell destruction and diabetes in a mouse diabetes model.
The monoclonal antibody 266-6K1 recognizes human myeloperoxidase (MPO), an ~135 glycoprotein expressed in all cells of the myeloid linage. MPO functions as an ?2β2 heteromultimer consisting of two heavy (?) and two light (β) chains of 55 and 15 kDa respectively. MPO is abundantly present in azurophilic granules of polymorphonuclear neutrophils (PMNs). It is an important enzyme used during phagocytic lysis of engulfed foreign particles which takes part in the defense of the organism through production of hypochlorous acid (HOCl), a potent oxidant. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. Upon activation of neutrophils, MPO can be rapidly released and as such useful in body fluids as marker for inflammatory status. Involvement of MPO has been described in numerous diseases such as atherosclerosis, lung cancer, Alzheimer's disease, inflammatory bowel disease and multiple sclerosis. Autoimmune antibodies to MPO (so called ANCA) are involved in Wegenerâs disease. Since the discovery of MPO deficiency, initially regarded as rare and restricted to patients suffering from severe infections, MPO has attracted more clinical attention.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
266-6K1
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
La Rocca; G et al. Basic Res Cardiol 2009; 104: 307
The monoclonal antibody 25F9 recognises a protein of 86 kD on the cell surface and within the cytoplasm of mature macrophages. The antibody is associated with fully differentiated tissue macrophages both in normal and in diseased tissues, independently of the presence or absence of inflammation. The antigen is absent on freshly isolated monocytes and other blood cells. After 6 to 7 days culture human monocytes become positive. Some melanoma and carcinoma cell lines are also positive. Furthermore the monoclonal antibody 25F9 cross reacts with a subpopulation of macrophages of rhesus monkey, pig alveolar macrophages and Kupffer cells. The monoclonal antibody 25F9 is very useful for macrophage phenotyping.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
25F9
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Rosseau; S et al. Am J Physiol Lung Cell Mol Physiol 2000; 279: L25
The monoclonal antibody RM3/1 recognizes CD163, a 130 kDa type I membrane glycoprotein, which is expressed exclusively in the monocyte/macrophage system. CD163 is a member of the cysteine-rich scavenger receptor superfamily. CD163 is an acute phase regulated receptor for the hemoglobin-haptoglobin complex. Another important function of CD163 seems to be in the adhesion of monocytes to activated endothelial cells. The expression levels of CD163 vary during the course of macrophage differentiation. The highest levels are found on tissue macrophages whereas bone marrow-derived cells are CD163 negative. CD163 positive cells include skin histiocytes, gut, Kupffer cells, and macrophages in spleen, thymus placenta and inflamed or tumorous tissues. The protein expression is markedly induced by glucocorticoids, IL-6 and IL-10 while down-regulated by cyclosporin A and by phorbol esters.<br /> CD163 can be cleaved to release to soluble form (sCD163). PMA can induce the shedding of sCD163. Intravenous lipopolysaccharide (LPS) produces a rapid rise of sCD163 in plasma of patient as it induces metalloproteinase-mediated shedding from monocytes surface. sCD163 in plasma is a parameter in diseases affecting macrophage function and monocyte/macrophage load in the body. The concentration of sCD163 is probably reflecting the number of macrophages of the 'alternative macrophage activation' phenotype with a high CD163 expression playing a major role in dampening the inflammatory response and scavenging components of damaged cells. As such sCD163 can be important as a disease marker in inflammatory conditions e.g. infection, autoimmune disease, transplantation, atherosclerosis and cancer.<br /> The monoclonal antibody RM3/1 can be used for macrophage phenotyping..The RM3/1 antigen expression is restricted to human monocytes and macrophages that evolve in the late phase of inflammation, predominantly macrophages of healing tissue. For macrophages in the synovialis of patients with rheumatoid arthritis; in alveolar macrophages and in Kupffer cells a double staining can be observed with the monoclonal antibody 25F9 (HM2158) recognizing- mature macrophages, which is not the case in other tissues.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
RM3/1
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Zwadlo; G et al. Expl Cell Biol 1987; 55: 295
References 2:
Högger, P et al J Immunol 1998, 161: 1883
References 3:
Droste; A et al. Biochem Biophys Res Commun 1999; 256: 110
References 4:
Frings W et al. FEBS Lett 2002; 526: 93
References 5:
Komohara Y et al. J Histochem Cytochem 2006; 54: 763
The monoclonal antibody 27E10 recognizes an epitope specific for the S100A8/A9 heterocomplex that is not exposed on the individual subunits S100A8 (MRP8, calgranulin-A) or S100A9 (MRP14, calgranulin-B). The calcium-binding migration inhibitory factor-related proteins, MRP-8 (S100A8) and MRP-14 (S100A9) belong to the S100 protein family. The expression of these proteins is largely confined to the cytosol of neutrophils and monocytes. The complex formation of these proteins is a calcium-dependent process. The S100A8/A9 heterocomplex, also called MRP-8/MRP-14 complex or calprotectin, comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. The S100A8/A9 complex has antibacterial, antifungal, immunomodulating and antiproliferative effects. Besides this it is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity, like inflammatory bowel disease. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore calprotectin is also detectable in feces. Elevated levels of calprotectin have been observed in body fluids such as plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infection and inflammatory conditions.<br> The monoclonal antibody 27E10 can be used for early detection of inflammatory macrophages, for the characterization of tumorous tissues and the monitoring of peripheral blood cell cultures. The antibody 27E10 does not react with lymphocytes or platelets.
The monoclonal antibody E9 reacts with Endoglin, a 190 kDa homodimeric transmembrane glycoprotein composed of disulfide-linked subunits. The external domain binds TGF-beta1 and -beta3 isoforms with high affinity. Two different isoforms (L and S) of CD105 with capacity to bind TGF-beta have been characterized, which differ in the amino acid composition of their cytoplasmic tails. Mutations in the gene encoding endoglin have been linked to the human disease hereditary hemorrhagic telangiectasia type 1 (HHT1), a vascular disorder characterized by localized vascular dysplasia and a tendency towards arteriovenous malformations. Mice expressing a single CD105 allele develop external signs of disease similar to human HHT1, supporting the haploinsufficiency model for HHT1. Mice lacking endoglin die from defective angiogenesis characterized by failure of vascular smooth muscle investment of embryonic blood vessels, suggesting a defect in vascular smooth muscle cell development. Microvessel density (MVD) has been reported to be an independent prognostic indicator of outcome in a variety of human malignancies, with increased MVD correlating with shorter overall and relapse-free survival rates. The MVD counts using anti-CD105 antibody significantly correlated with overall and disease-free survival. Anti-CD105 monoclonal antibody E9 and anti-CD34 monoclonal antibody have been successfully used to quantify MVD in human breast carcinoma. The monoclonal antibody E9, directed against CD105, has also been used as a prognostic marker for primary central nervous system lymphomas.
C-terminal Src kinase (Csk) is a non-receptor protein tyrosine kinase which resembles Src-family kinases, but unlike them lacks the conserved autophosphorylation site, the regulatory C-terminal tyrosine as well as myristylation and palmitylation. Csk negatively regulates Src-family kinases by phosphorylation of their C-terminal regulatory tyrosine. Disruption of the csk gene causes constitutive activation of Src-family kinases, and overexpression of Csk usually counteracts their signaling. The Csk-mediated regulation of those Src-family kinases that are localized in lipid rafts is enabled by a ubiquitously expressed transmembrane adaptor PAG (also known as Cbp, Csk-binding protein), which recruits Csk.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
CSK-04
Concentration:
n/a
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Human transferrin receptor 1 (TfR1), also designated CD71, is a homodimeric type II membrane glycoprotein of 90-95 kDa. This receptor binds two molecules of the serum iron-transport protein transferrin (Tf) and is internalised into endosomes that are acidified, resulting in the release of iron from Tf. TfR1 is not expressed on resting leukocytes but is upregulated on all proliferating cells upon activation, reflecting the iron dependence of proliferation. In tissues TfR1 is expressed on most dividing cells and on brain capillary endothelium. Expression of TfR1 is down regulated as a result of iron overload. TfR1 shares 45% identity with TfR2.
SLP76 (SH2 domain-containing leukocyte protein of 76 kDa) is a cytosolic adaptor protein which translocates to the plasma mambrane and is involved in multiple signaling pathways in T cells, mast cells, neutrophils and platelets; B cells express its analog SLP65/BLNK (B cell linker protein). SLP76 is phosphorylated by Syk-family and Tec-family tyrosine kinases and couples them to the phosphorylation and activation of PLC-gamma. Via Gads or Grb2, SLP76 also associates with LAT adaptor by involvement of SLP76 proline-rich region. The SH2 domain of SLP76 has been identified as the region involved in binding the serine/threonine kinase HPK1. HPK1 may act as both a positive and a negative regulator by promoting the Jnk-mitogen activated protein kinase (MAPK) pathway and inhibiting the pathway leading to AP-1 activation.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
SLP-76/3
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Monoclonal antibody FA6-152 recognizes human CD36 (88-kDa), a cell surface class B scavenger receptor, also known as thrombospondin receptor CD36 is a heavily N-glycosylated transmembrane protein of ~88 kDa with two short intracellular domains and a large extracellular domain. The protein is sensitive for neuroaminidase, resulting in a shift from 88 to 85 kDa. CD36 is expressed on platelets, mature monocytes and macrophages, microvascular endothelial cells, mammary endothelial cells, during stages of erythroid cell development and on some macrophage derived dendritic cells. The antibody recognizes adult and fetal monocytes, platelets and reticulocytes, but doesnât stain lymphocytes and granulocytes. Reactivity has also been found in small intestine, kidney, liver and thyroid. CD36 expression is primarily controlled by the transcription heterodimer PPARg-RXR (peroxisome proliferator-activated receptor-g-retinoid-X-receptor). CD36 is preferentially found within lipid rafts, which facilitates its association with receptors, signaling and adaptor molecules. It is a receptor and transporter of oxidized lipids and long chain fatty acids. CD36 has been implicated in many biological processes including angiogenesis, phagocytosis, inflammation, and lipid and glucose metabolism. Several in vivo models support the role of the thrombospondin / CD36 system in angiogenesis and tumor growth. An important role for CD36 has been found in Malaria as major receptor for P. falciparum-infected red blood cells. CD36 is associated with Src-family kinases and with the integrins ?3β1 and ?6β1. Recently, CD36 has been identified as a protein that is required for toll like receptor (TLR2) recognition of di-acylated bacterial lipopeptides and lipoteichoic acid4. Furthermore, CD36 has been shown to function as phagocytic receptor for apoptotic cells. Many different ligands have been reported to interact with CD36, suggesting that CD36 could recognize a structure-based domain rather than specific contact residues. Monoclonal antibody FA6-152 blocks the biological activity of CD36 by blocking collagen/thrombospondin binding. The antibody agglutinates fetal but not adult erythrocytes.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
FA6-152
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Edelman P et al. Blood 1986; 67: 56
References 2:
Kieffer N et al Biochem J 1989, 262: 835
References 3:
Thibert V et al. Thromb Haemost 1992; 68: 600
References 4:
Nakata A et al. Arterioscler Thromb Vasc Biol 1999; 19: 1333
LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
LIME-10
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
LIME-06
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
For IHC-P protease treatment and/or microwave treatment recommended). The antibody reagent represents an excellent marker to study palmoplantar epidermal distribution and differentiation. Specifically reactive in the middle/upper suprabasal layers (stratum spinosum/ granulosum) of the epidermis of palm and sole. K9 can be detected in primary cultures of palmoplantar keratinocytes when they shift to differentiation-promoting conditions and grow stratified (upper cells). K9 has not been found in normal, i.e. non-pathogenic, non-ridged epidermis, beside some minor cells surrounding the acrosyringeal ducts. No labelling has been found in epithelial cells of other stratified epithelia such as oesophagus or complex epithelia (e.g. urothelium) or in ductal or simple epithelia. Negative tissues include: muscle, liver and duodenum. The antibody cocktail is an excellent tool to characterize primary cultures of keratinocytes/skin transplants for application in burn treatment.
ACTH (same as corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. 2F6 is specific to synacthen (aa 1-24 of ACTH); does not react with CLIP (aa 17-39 of ACTH). ACTH is also produced by cells of the immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
2F6
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Kimitsuki K. et al. J Vet Med Sci 76(1) 133-138 (2014).
The antibody represents an excellent marker for non-keratinized squamous epithelia and proliferating cells of epidermis (e.g. within psoriatic lesions).Polypeptide Reacting: Mr 56 000 polypeptide (cytokeratin 6) of human squamous epithelia. Tissues and Tumors specifically detected: The antibody is suitable for discrimination of keratinizing and non-keratinizing squamous cell carcinoma versus e.g. poorly differentiated adenocarcinoma.
The monoclonal antibody TLR3.7 recognizes the 116 kDa human Toll-like receptor 3 (TLR3, CD283). Toll-like receptors (TLRs) are highly conserved from Drosophila to humans and share structural and functional similarities. TLRs constitute of a family of pattern recognition receptors (PRRs) that mediate cellular responses to a large variety of pathogens (viruses, bacteria, and parasites) by specific recognition of so-called âpathogen-associated molecular patternsâ. Activation of TLRs, a family of at least 11 different members that function either as homo- or heterodimers, leads to activation of NFκBdependent and IFN-regulatory factor-dependent signaling pathways. TLRs have a central role in innate immunity and are also required for the development of an adaptive immune response. TLRs are expressed by various cells of the immune system, such as macrophages and dendritic cells. TLRs are class I receptors, with a single ?-helix that spans the cell membrane. They recognize and respond to molecules derived from bacterial, viral and fungal pathogens, such as lipopolysaccharide (LPS) from the outer membrane of Gram negative bacteria, peptidoglycan fragments from bacterial cell walls and single-stranded and double-stranded RNA from viruses.<br /> Some forms of RNA and DNA from pathogens exhibit immutable features that distinguish them from nucleic acids of higher organisms. For example, dsRNA, is a common intermediate of viral replication and a potent indicator of infection. Toll-like receptor 3 (TLR3) recognizes viral double-stranded RNA and its synthetic analog polyriboinosinic:polyribocytidylic acid (poly(I:C)). TLR3 is normally located in acidic endosomes where its luminal ectodomain (ECD) encounters dsRNA and induces type I interferon (IFN), inflammatory cytokine/chemokine production and dendritic cell (DC) maturation via the adaptor protein TICAM-1 (also called TRIF). Based on the different subcellular localization of cytosolic RNA receptors and TLR3, these receptors seem to play distinct roles in anti-viral immune responses.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
TLR3.7
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Matsumoto; M et al. Biochem Biophys Res Commun 2002; 293: 1364
References 2:
Oshiumi, H et al Nat Immunol 2003, 4: 161
References 3:
Matsumoto; M et al. J Immunol 2003; 171: 3154
References 4:
Burgener I et al. Vet Immunol Immunopathol 2008; 124
5H4-E2 reacts with hCG beta chain, which is specific for hCG. The alpha chain is identical among hCG, TSH, FSH and LH. While hCG is secreted in large quantities by normal trophoblasts, it is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. Besides trophoblastic tumors e.g. choriocarcinoma, large cell carcinoma, adenocarcinoma and squamous cell carcinoma of the lung are also positive in 90%, 60% and 20% of cases respectively. In hCG producing tumors, including also certain testicular and embryonic carcinomas, the beta chain is produced in higher quantities than the alpha or dimeric chains. hCG expression by non-trophoblastic tumors may indicate aggressive behavior.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
5H4-E2
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
McDonald EA et. al. Endocrinology 150:4358-65 (2009)
Thyrotropin (hTSH) promotes the growth of the thyroid gland in the neck and stimulates it to produce more thyroid hormones. hTSH is composed of two subunits - alpha nad beta.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
TSH-116
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Thyrotropin (hTSH) promotes the growth of the thyroid gland in the neck and stimulates it to produce more thyroid hormones. hTSH is composed of two subunits - alpha nad beta.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
TSH-51
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Choriogonadotropin (hCG) is a protein of the molecular weight about 40 kDa. It belongs to the glycoprotein hormone family together with lutropin (LH), follitropin (FSH) and thyrotropin (TSH). Choriogonadotropin is synthesised by trophoblastic cells of the placenta during pregnancy and stimulates the growth of corpus luteum. The other hormones are produced by anterior pituitary gland. All these hormones are structurally related, being composed of two noncovalently associated subunits alpha and beta.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
HCG-61
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Plakoglobin, also known as gamma-catenin belongs together with alpha- and beta catenin to the catenin family. Catenins mediates cell-cell adhesion by interaction with cadherins. Plakoglobin is found in desmosomes and adherens junctions. Plakoglobin is highly homologous to beta-catenin although its function differs from that of beta-catenin. Whereas beta-catenin has been found in potentiating hyperproliferation and tumor formation, plakoglobin can suppress tumorigenicity. Overexpression of plakoglobin has been shown to suppress cell proliferation and cell tumorigenicity in animals. Furthermore reduced plakoglobin expression has been found in tumor tissues and metastatic lesions of renal cells, esophageal carcinomas and in skin carcinomas. The monoclonal antibody 15F11 cross reacts with rat and weakly with mouse.
Desmoglein 3 is a member of the desmosomal cadherin family that comprise the desmogleins and desmocollins. Desmosomes are cell-cell junctions in most epithelial cells and serve to link the intermediate filament cytoskeletons of adjacent cells. Within the desmosome the extracellular domain of desmoglein is essential for calcium dependent heterophilic binding to the desmocollins, whereas the intracellular domain is essential for binding to the desmosomal plaque protein, plakoglobin. Desmoglein 3 is synthesized in both the basal and the lower suprabasal compartments. The monoclonal antibody 5G11 reacts with the 140 kDa desmoglein 3 protein. Furthermore it is not cross reactive with desmoglein 1 or desmoglein 2.
The monoclonal antibody 6D8 recognizes the extracellular domain of human desmoglein-2. Desmogleins and desmocollins are members of the cadherin family of transmembrane proteins that together make up the core of the desmosome, a structure that provides transmembrane strength to tissues undergoing mechanical stress. The desmosomal cadherins, desmogleins and desmocollins, mediate calcium-dependent cell-cell adhesion by forming homotypic and heterotypic interactions with one another. The multiprotein desmosomal complex also includes the cytoplasmic desmosomal plaque proteins plakoglobin, phakophilins, and desmoplakin, which bind to the intracellular domain of the desmogleins and function to anchor the keratin intermediate filament network to site of cellcell contacts.<br /> In human, four desmogleins have been identified (Dsg14). Desmogleins are synthesized with a signal peptide that directs them to the endoplasmic reticulum and a proregion that is removed during protein processing. The mature protein includes four highly conserved extracellular domains (EC 14) and a fifth membrane proximal, more variable EC domain that is referred to as the &ldquo;extracellular anchor domain. Desmoglein-2 is expressed on various cells including simple epithelia and myocardium, tumors and and many cell cultures.<br /> Desmogleins play critical roles in cell adhesion and skin blistering diseases, as they are the target antigens of autoimmune antibodies and bacterial toxins. Desmosomal dysfunction has been implicated in a number of diseases, including striate palmoplantar keratoderma, skin fragility, and ectodermal dysplasia, and most recently arrhythmic right ventricular cardiomyopathy (ARVC).
Desmoglein 1 is a member of the desmosomal cadherin family that comprise the desmogleins and desmocollins. Desmosomes are cell-cell junctions in most epithelial cells and serve to link the intermediate filament cytoskeletons of adjacent cells. Within the desmosome the extracellular domain of desmoglein is essential for calcium dependent heterophilic binding to the desmocollins, whereas the intracellular domain is essential for binding to the desmosomal plaque protein, plakoglobin. Desmoglein 1 is synthesized exclusively in the suprabasal layers. Intact and functionally active desmoglein 1 is essential to epidermal integrity. The monoclonal antibody 27B2 reacts with the 165 kDa desmoglein 1 protein. Furthermore it is not cross reactive with desmoglein 2 or desmoglein 3.
Beta-catenin belongs together with alpha- and gamma-catenin to the catenin family. Catenins mediates cell-cell adhesion by interaction with cadherins. Beta-catenin is highly homologous to gamma-catenin (plakoglobin) although its function differs from that of plakoglobin. Whereas plakoglobin has been found to suppress tumorigenicity, beta-catenin potentiates hyperproliferation and tumor formation. In the nucleus beta-catenin it complexes with transcription factors and thus regulates the expression of specific genes. By its dual role, i.e. a structural role in cell-cell junctions and a regulatory role in the nucleus, beta-catenin can transduce changes in cell adhesion and junction formation to control transmembrane signalling and gene expression.
The monoclonal antibody M.Ab.F11 recognizes junctional adhesion molecule-A (JAM-A) also known as the human platelet F11-Receptor (F11R) or JAM-1. JAM-A is a surface glycoprotein duplex (32 and 35 kDa) belongingto the immunoglobulin superfamily found on the surface of human platelets and at intercellular junctions of endothelial cells and epithelial cells. JAM-A belongs together with JAM-C (JAM-2) and JAM-B (VE-JAM or JAM-3) to a family of adhesion proteins with a V-C2 immunoglobulin domain organization. JAM-A plays an important role in tight junctions where it is involved in cell-to-cell adhesion through homophilic interactions. It co-distributes with other tight junction components such as ZO-1, 7H6 antigen, cingulin and occludin. Moreover, JAM-A plays a role in platelet aggregation, secretion, adhesion, spreading.<br /> In the platelet, JAM-A is a membrane protein involved in 2 distinct processes initiated on the platelet surface. Namely,, antibody-induced platelet aggregation and secretion both dependent on FcgammaRII and GPIIb/IIIa integrin, a process that may be involved in pathophysiological processes associated with certain thrombocytopenias and secondly, antibody mediated platelet adhesion independent from FcgammaRII or- fibrinogen receptor that appears to play a role in physiological processes associated with platelet adhesion and aggregation. A physiological role for the JAM-A protein was demonstrated by its phosphorylation after the stimulation of platelets by thrombin and collagen. A pathophysiological role for the JAM-A was revealed by demonstrating the presence of JAM-A antibodies in patients with thrombocytopenia. Adhesion of platelets through JAM-A resulted in events characteristic of the action of cell adhesion molecules. Recent data suggests a role for JAM-A in the adhesion of platelets to cytokine-inflamed endothelial cells and thus in thrombosis and atherosclerosis induced in non-denuded blood vessels by inflammatory processes.
The monoclonal antibody BV16 recognizes the human junction adhesion molecule (JAM)-A. Together with JAM-C (JAM-2) and JAM-B (VE-JAM or JAM-3), JAM-A belongs to a family of adhesion proteins with a V-C2 immunoglobulin domain organization and their molecular weight is about 30-40 kDa. JAMs are important for a variety of cellular processes, including tight junction assembly, leukocyte transmigration, platelet activation, angiogenesis and virus binding. JAM-A is expressed by endothelial and epithelial cells, platelets, neutrophils, monocytes, lymphocytes and erythrocytes. Like all other JAMs, JAM-A plays an important role in tight junctions where it is involved in cell-to-cell adhesion through homophilic interaction. It codistributes with other tight junction components as ZO-1, 7H6 antigen, cingulin and occludin. JAM-A also plays an important role in leukocyte transmigration. Leukocyte transmigration can be blocked by antibodies and by soluble JAM-A/Fc fusion proteins. Homophilic JAM-A interactions between leukocytes and the endothelium but also heterophilic interactions of JAM-A with the beta2-integrin leukocyte function-associated antigen-1 (LFA-1) are considered to actively guide leukocytes during transmigration. Several studies imply a role for JAM-A in the initiation of atherosclerosis since JAM-A is upregulated on early atherosclerotic endothelium. The adhesion of activated platelets on the activated endothelium is mediated by homophilic interactions of JAM-A.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
BV16
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Bazzoni; G et al. J Biol Chem 2000; 275: 20520
References 2:
Luo, Y et al Invest Ophthalmol Vis Sci 2006, 47: 3644
7LE reacts with Lewis a blood group antigen, a carbohydrate determinant present on both glycolipids and glycoproteins, expressed a.o. in colonic and kidney epithelial cells. Lewis a may be useful for detection of gastrointestinal, pancreatic and colorectal carcinomas.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
7LE
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Creuzot-Garcher et al. Invest Oph(thalmol Vis Sci. 40(8):1631-6 (1999)
References 2:
Torrado et al. Cancer Epidemiol Biomarkers Prev. 1(3): 199-205 (1992)
References 3:
Torrado et al. Gastroenterology, 102 (2): 424-430 (1992)
Monoclonal antibody BV1 recognizes human vitronectin. Vitronectin is an abundant glycoprotein (~75 kDa), consisting of 459 amino acids. About one third of the protein molecular mass is composed of carbohydrates. Vitronectin is found in blood plasma and the extracellular matrix. Vitronectin is a multifunctional protein, since it promotes attachment and spreading of animal cells in vitro, it inhibits cytolysis by the complement C5b-9 complex, and it modulates antithrombin III-thrombin action in blood coagulation. The protein consists of three domains: the N-terminal Somatomedin B domain (1-39), a central domain with hemopexin homology (131-342) and a C-terminal domain (347-459) also with hemopexin homology. The Somatomedin B domain binds to Plasminogen Activator Inhibitor-1 (PAI-1) and is responsible for PAI-1 stabilization. Furthermore, the Somatomedin B domain can also interact with the urokinase plasminogen activator receptor (uPAR). Vitronectin-uPAR interaction is required and sufficient to initiate downstream changes in cell morphology, migration and signal transduction. High plasma levels of both PAI-1 and uPAR have been shown to correlate with a negative prognosis for cancer patients. Additionally, vitronectin is a component of platelets and is as such involved in hemostasis. Amino acid 45-47 (RGD) are capable of binding to membrane bound integrins, which serve to anchor cells to the extracellular matrix. Vitronectin in plasma is an inactive monomer form. In contrast, tissue vitronectin is an active multimeric form and is able to interact with various matrix ligands like proteoglycans and collagen. Mice with a genetic deletion of vitronectin show delayed wound healing, suggesting an important role of vitronectin in tissue remodeling after injury. The monoclonal antibody BV1 binds to soluble vitronectin as well as to membrane bound vitronectin.
2-25LE reacts with Lewis b antigen which is highly expressed in stomach, colon, small intestine, lung and kidney and to a lesser extent in salivary gland, bladder, uterus and liver
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
2-25LE
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bara et al. Cancer Res. 46: 3983-3898 (1986)
References 2:
Bara et al. Biochem. J. 254: 185-193 (1988)
References 3:
Creuzot-Garcher et al. Invest Oph(thalmol Vis Sci. 40(8):1631-6 (1999)
References 4:
Good et al. Vox Sanguinis 62(3): 180-189 (1992)
References 5:
Torrado et al. Gastroenterology, 102 (2): 424-430 (1992)
Cardiotin is a high molecular weight protein complex (300 kDa) located in the mitochondria of cardiomyocytes and skeletal muscle. The cardiotin structure exists of subunits of 60 kDa and 100 kDa, probably in a tetrameric configuRation. Both subunits contain the same amino-terminal 14 amino-acid sequence, showing high homology to Human skeletal muscle ?-actinin. During cardiac contractile dysfunction and myocard cell differentiation, the cardiotin distribution is affected. Compared to other structural proteins, cardiotin is one of the first to respond to insults (ischemia, fibrillation) that influence the functional status of cardiomyocytes. SR-2 reacts with cardiomyocytes, skeletal muscle, stromal and epithelial cells as well in vivo as in vitro.
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in Human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 7.8. The individual Human Cytokeratins are numbered 1 to 20. The various epithelia in the Human body usually express Cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of matuRation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The Cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. RCK107 reacts exclusively with Cytokeratin 14 which is present in basal cell compartments of stRatified and combined epithelia.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
RCK107
Concentration:
1 mg/ml
Storage buffer:
PBS with 0.09% sodium azide
Storage:
2-8°C
References 1:
Wetzels et al. Am J Pathol 1991;138:751-763
References 2:
Smedts et al. Am J Pathol 1992;140:601-612
References 3:
Bauwens et al. Ann Otol Rhinol Laryngol 1992;101:479-486
References 4:
van Leenders et al. Lab Invest 2000;80:1251-8
References 5:
Spies et al. Vos et al. Vet Pathol 1993;30:352-361
Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
C-11
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Cytokeratin pan is part of a subfamily of intermediate filament proteins that are characterized by remarkable biochemical diversity, and represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight between 40 kDa- 68 kDa, and an isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. The composition of cytokeratin pairs vary with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
AE-3
Concentration:
1 mg/ml
Storage buffer:
PBS with 0.1% sodium azide
Storage:
2-8°C
References 1:
Woodcock-Mitchell, J., et al., J. Cell Biol. 95, 580-588 (1982).
References 2:
Tseng, S.C.G., et al., Cell 30, 361-372 (1982).
References 3:
Eichner, R., et al., J. Cell Biol. 98, 1388-1396 (1984)
References 4:
Sun, T.-T., et al., The Cancer Cell 1,169-176 (1984)
Cytokeratin pan is part of a subfamily of intermediate filament proteins that are characterized by remarkable biochemical diversity, and represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight between 40 kDa- 68 kDa, and an isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. The composition of cytokeratin pairs vary with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
AE-1
Concentration:
1 mg/ml
Storage buffer:
PBS with 0.1% sodium azide
Storage:
2-8°C
References 1:
Woodcock-Mitchell, J., et al., J. Cell Biol. 95, 580-588 (1982).
References 2:
Tseng, S.C.G., et al., Cell 30, 361-372 (1982).
References 3:
Eichner, R., et al., J. Cell Biol. 98, 1388-1396 (1984)
References 4:
Sun, T.-T., et al., The Cancer Cell 1,169-176 (1984)
Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratin 18 belongs to type I family (acidic cytokeratins).
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
DA-7
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
C-43
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratin 18 belongs to type I family (acidic cytokeratins).
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
C-04
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
BA-17
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
C-51
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Marker for the differentiation of 160 kD neurofilament peptide from other intermediate filament proteins. The antibody is useful for studying neurofilament expression and for tumor characterization (pheochromocytoma, paraganglioma, ganglioneuroblastoma, ganglioneuroma and other tumors of neuronal origin). Positive control: human brain tissue
Marker for the differentiation of 200 kD neurofilament peptide from other intermediate filament proteins. The antibody is useful for studying neurofilament expression and for tumor characterization (pheochromo-cytoma, paraganglioma, ganglioneuroblastoma, gan-glioneuroma and other tumors of neuronal origin). Positive control: Human brain tissue.
Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
C-50
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in Human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 7.8. The individual Human Cytokeratins are numbered 1 to 20. The various epithelia in the Human body usually express Cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of matuRation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The Cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
RGE 53 specifically recognizes simple and glandular epithelial cells from the gastrointestinal tract, the respiratory tract and the urogenital tract, as well as endocrine and exocrine tissues and myoepithelial cells. No reaction with stratified squamous epithelia. In the transitional epithelium of the bladder RGE 53 reacts only with superficial (umbrella) cells. The antibody is useful for the discrimination of adenocarcinomas and mesotheliomas from squamous cell carcinomas and non-epithelial tumors. Polypeptide reacting: 45 kD keratin K18 (formerly also designated cytokeratin 18). Positive control: Adenocarcinoma
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
RGE53
Concentration:
n/a
Storage buffer:
Contains 0.1% sodium azide
Storage:
2-8°C
References 1:
Ramovs V et al. Breast Cancer Res 2019;21:63
References 2:
Herman CJ et al. Am J Pathol 1985;120:419
References 3:
Puts JJ et al. Int J Gynecol Pathol 1985;4:300-313
The antibody specifically recognizes the 56.6 kD keratin K10 (formerly also designated cytokeratin 10) polypeptide in squamous cells and carcinomas from e.g. epidermis, lung, bladder, cervix, esophagus.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
DE-K10
Concentration:
n/a
Storage buffer:
Contains 0.09% sodium azide
Storage:
2-8°C
References 1:
Larribere, L. et al. Stem.Cell.Reports 2017;9:1234-1245
References 2:
Langbein, L. et al. Cell Tissue Res. 2013;354:793812
References 3:
Wallace, L. et al. , J. Cell Sci.2012;125:17501758
References 4:
Langbein, L. et al. J. Invest. Dermatol2005;125:428444
References 5:
Boehnke, K. et al. Eur. J. Cell Biol.2007;86: 731746
Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratin 18 belongs to type I family (acidic cytokeratins).
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
DC-10
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Cytokeratin 19 is found in most simple epithelia and non keratinised squamous epithelia with predominant focal reactivity. RCK108 stains practically all epithelial tumours, especially adenocarcinoma. It is negative in hepatocellular carcinoma (4). Most medullary, weakly differentiated ductal carcinoma are also CK19-negative (3). Cytokeratins are a group of water insoluble filament proteins, which are constituents of the cytoskeleton of epidermal cells and other epithelial cells. By gel electrophoretic analysis up to now 20 different cytokeratins have been characterized. They have been divided into basic and acid subfamilies and may also be distinguished by their molecular weights and their tissue distribution. The most frequently used nomenclature has been published by R. Moll et al. (1982). Antibody RCK108 reacts with a 40 kDa keratin (Cytokeratin 19) in immunoblot.
Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
C-46
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
The antibody does not stain non-cornifying squamous epithelium and transitional epithelium of the bladder. In immunoblotting experiments the antibody recognizes only keratin no. 13 (54 kDa).
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
2D7
Concentration:
15 ug/ml
Storage buffer:
Culture medium with 0.01M Sodium Azide
Storage:
2-8°C
References 1:
Muijen GNP et al. (1986) Exp Cell Res 162, 97-113
References 2:
Niekerk CC van et al. (1991) J Pathol 165, 145
References 3:
Ramaekers F (1990) Am J Pathol 136, 641
References 4:
Niekerk CC van et al. (1989) Int J Cancer 43, 1065
The antibody does not stain non-cornified squamous epithelia, except cornea and transitional epithelial regions, with the exception of basal cell layers of some stratified epithelia (ref. 1). In immunoblotting experiments the antibody was shown to recognize only cytokeratin no. 13 (54 kDa).
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
1C7
Concentration:
15 ug/ml
Storage buffer:
Culture medium with 0.01M Sodium Azide
Storage:
2-8°C
References 1:
Muijen GNP et al. (1986) Exp Cell Res 162, 97-113
References 2:
Muijen GNP et al. (1987) Exp Cell Res 171, 331-345
The antibody stains non-cornifying squamous epithelium and transitional epithelium of the bladder, cells in certain ciliated pseudo-stratified epithelia and ductal epithelia of various exocrine glands. In immunoblotting experiments the antibody was shown to recognize only cytokeratin no.4 (59 kDa).
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
6B10
Concentration:
15 ug/ml
Storage buffer:
Culture medium with 0.01M Sodium Azide
Storage:
2-8°C
References 1:
Muijen GNP et al. (1986) Exp Cell Res 162, 97-113
References 2:
Muijen GNP et al. (1987) Exp Cell Res 171, 331-345
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in Human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 7.8. The individual Human Cytokeratins are numbered 1 to 20. The various epithelia in the Human body usually express Cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used in the distinction of different types of epithelial malignancies. The Cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
OVTL12/30
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Ramaekers, F. et al. 1990 Am J Pathol 136, 641-55
References 2:
van Niekerk, CC et al. 1991 J Pathol 165, 145-52
References 3:
van de Molengraft, F.J et al. 1993 Histopathology 22, 35-8
References 4:
van Niekerk, CC et al. 1997 Cancer Detect Prev 21, 247-57
GFAP (glial fibrillary acidic protein) was discovered by Bignami et al. (1972) as a major fibrous protein of multiple sclerosis plaques. It was subsequently found to be a member of the 10 nm or intermediate filament protein family, specifically the intermediate filament protein family class III, which also includes peripherin, desmin and vimentin. GFAP is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. It is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. Although its function is not fully understood, GFAP protein is probably involved in controlling the shape and movement of astrocytes. The protein probably also plays a significant role in the interactions of astrocytes with other cells, which are required for the formation and maintenance of the insulating layer (myelin) that covers nerve cells. Additionally, GFAP protein may assist in maintaining the protective barrier that allows only certain substances to pass between blood vessels and the brain (blood-brain barrier).In adults, GFAP levels increase as a result of the proliferation of astrocytes that occurs in a response to a variety of physical, chemical and etiological insults, including Alzheimer’s disease, epilepsy and multiple sclerosis.Antibodies to GFAP are therefore very useful as markers of astrocytic cells and neural stem cells and for distinguishing of neoplasms of astrocytic origin from other neoplasms in the central nervous system. Finally, Alexander's disease was recently shown to be caused by point mutations in protein coding region of the GFAP gene (Brenner et al., 2001). All forms of Alexander disease are characterized by the presence of Rosenthal fibers, which are GFAP containing cytoplasmic inclusions found in astrocytes.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
GF-01
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs in most vertebrates are composed of three different polypeptide chains with different molecular weights – neurofilament heavy protein (NF-H), medium (NF-M) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. The protein segment on the C-terminal side of the human NF-H rod is uniquely long (more than 600 amino acids) compared to other IF proteins and is highly charged (> 24 % Glu, > 25 % Lys), rich in proline (> 12 %) and improverished in cysteine, methionine and aromatic amino acids. Its most remarkable feature is a repetitive sequence that covers more than half its lenght and includes the sekvence motif Lys-Ser-Pro (KSP) greater than 40 times. Plasma neurofilament heavy chain level has been proposed as a marker of axonal injury and clinical use of its degeneration and loss has been suggested as a biomarker of several neurodegenerative diseases.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
NF-01
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Mab RCK 102, directed against the 58 kD and 52.2 kD keratin proteins (keratins K5 and K8; formerly also designated cytokeratins 5 and 8 in the Moll catalogue of keratins), reacts with all epithelial cell types. No reaction is observed in non-epithelial tissues. The antibody can be used for the discrimination of carcinomas from non-epithelial tumors such as lymphomas, sarcomas and neural tumors. RCK 102 is applicable on frozen and paraffin-embedded tissue. Formalin fixation may cause antigen denaturation or masking. Therefore, protease digestion is necessary prior to antibody application. Positive control: Skin, cervix.
The antibody does stain especially simple epithelia. Only frozen sections can be used in immunoperoxidase and immunofluorescence tests. In immunoblotting experiments the antibody was shown to recognize only keratin no.18 (45 kDa).
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
M9
Concentration:
15 ug/ml
Storage buffer:
Culture medium with 0.01M Sodium Azide
Storage:
2-8°C
References 1:
Muijen GNP van et al. (1987) Exp Cell Res 171, 331-345
The antibody does stain all cells that contain vimentin. Only frozen section can be used. The antibody only stains the 57kDa vimentin band in immunoblots performed on a lysate of human lymphocytes.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
V9
Concentration:
25 ug/ml
Storage buffer:
Culture medium with 0.01M Sodium Azide
Storage:
2-8°C
References 1:
Muijen GNP van (1984) Am J Pathol 116, 363-369
References 2:
Debus E et al. (1984) Eur J Cell Biol 34, 137-143
References 3:
Muijen G van (1986) Exp Cell Res 162; 97-113
References 4:
Corver WE et al. (1994) Cytometry 15, 117-128
References 5:
Broek JCM van den et al. (2000) Appl Immunohis & Mol Morphology 8, 316-321
The antibody does stain neurons in sections of brain and other tissues. On an immunoblot on a crude human brain extract the antibody will only stain the 70 kDa band of neurofilament (ref. 1)
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
2F11
Concentration:
15 ug/ml
Storage buffer:
Culture medium with 0.01M Sodium Azide
Storage:
2-8°C
References 1:
Muijen GNP van et al. (1984) Am J Pathol. 116, 363-369
References 2:
Klück P et al. (1984) Lancet (March 24th), 652-653
References 3:
Muijen GNP van et al. (1985) Hum Pathol 16, 590-595
References 4:
Banks-Schlegel SP et al. (1985) Cancer Res 406, 339
The antibody does stain all types of keratin containing (that is epithelial) cells in frozen sections of various tissues, with the exception of myo-epithelial cells and the podocytes of the glomeruli.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
80
Concentration:
15 ug/ml
Storage buffer:
Culture medium with 0.01M Sodium Azide
Storage:
2-8°C
References 1:
Muijen GNP van (1984) Am J Pathol 114, 9
References 2:
Muijen GNP van (1984) Am J. Pathol 116, 363
References 3:
Muijen GNP van (1985) Hum Pathol 16, 590
References 4:
Corver WE et al. (2000) Cytometry 41, 73-80
References 5:
Broek, JCM van et al. (2000) Appl. Immunohis. & Mol. Morph. 8, 316-321
The antibody stains cells containing GFAP. The antibody only stains the 56kD GFAP band in immunoblots performed on crude brain extract. It reacts also with mouse GFAP
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
6F2
Concentration:
25 ug/ml
Storage buffer:
Culture medium with 0.01M Sodium Azide
Storage:
2-8°C
References 1:
Muijen GNP van et al. (1987) Lab Invest 57, 359-369
This antibody is highly reactive with desmin. On immunoblots only the 52 kD desmin band is stained. Immunogen derived from human Leiomyoma. No cross reactivity with GFAP, Keratin, Vimentin or Neurofilament detected.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
D33
Concentration:
20 ug/ml
Storage buffer:
Culture medium with 0.01M Sodium Azide
Storage:
2-8°C
References 1:
Muijen GNP van et al. (1987) Lab Invest 57, 359-369
References 2:
Seidal T et al. (1987) Appl Pathol 5, 201-219
References 3:
Ramaekers F et al. (1983) Histochemical J 15, 691-713
References 4:
Broek JCM van et al. (2000) Appl Immunohis & Mol Morph 8, 316-321
Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The Cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. RCK103 is a Cytokeratin antibody recognizing (amongst others) Cytokeratin 5. This monoclonal antibody stains basal cells in combined and stRatified epithelial tissues. It recognizes the stem cell population, including the so-called amplifying cells in the prostate epithelium.
A synthetic peptide corresponding to a sequence at the N-terminus of human RAGE, different from the related mouse and rat sequences by six amino acids.
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml. Background: The receptor for advanced glycation end products (RAGE) is a multi-ligand member of the immunoglobulin superfamily of cell surface molecules. It interacts with distinct molecules implicated in homeostasis, development and inflammation, and certain diseases such as diabetes and Alzheimer's disease. RAGE is also a central cell surface receptor for amphoterin and EN-RAGE. And RAGE is associated with sustained NF-kappaB activation in the diabetic microenvironment and has a central role in sensory neuronal dysfunction. Moreover, RAGE propagates cellular dysfunction in several inflammatory disorders and diabetes, and it also functions as an endothelial adhesion receptor promoting leukocyte recruitment. Subcellular Localization: Tissue Specificity:
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml. Background: Aspartate aminotransferase, cytoplasmic is an enzyme that in humans is encoded by the GOT1 gene. Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Subcellular Localization: Tissue Specificity:
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml. Background: Kelch-like protein 12 is a protein that in humans is encoded by the KLHL12 gene. This gene encodes a member of the KLHL (Kelch-like) family of proteins. This protein has been identified as an autoantigen in the autoimmune disease Sjogren's syndrome and as a potential biomarker in primary biliary cirrhosis. This protein may act as a substrate adaptor of the Cullin-3 ubiquitin ligase complex to promote substrate-specific ubiquitylation. Ubiquitylation by this complex has been shown to regulate the Wnt signaling pathway as well as COPII vesicle coat size. A pseudogene has been identified on chromosome 22. Alternative splicing results in multiple transcript variants. Subcellular Localization: Tissue Specificity:
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml. Background: Transcription factor Sp1, also known as specificity protein 1* is a protein that in humans is encoded by the SP1 gene. The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. Subcellular Localization: Tissue Specificity:
E.coli-derived human Lamin A/C recombinant protein (Position: Y481-Y646). Human Lamin A/C shares 90% and 92% amino acid (aa) sequence identity with mouse and rat Lamin A/C, respectively.
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml. Background: Lamins are structural protein components of the nuclear lamina, a protein network underlying the inner nuclear membrane that determines nuclear shape and size. There are three types of lamins, A,B and C. The lamin A/C (LMNA) gene contains 12 exons. Alternative splicing within exon 10 gives rise to two different mRNAs that code for pre-lamin A and lamin C. Lamin A/C is mapped to 1q21.2-q21.3 and mutations in this gene cause a variety of human diseases including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy, and Hutchinson-Gilford progeria syndrome. Lamin A/C deficiency is thus associated with both defective nuclear mechanics and impaired mechanically activated gene transcription. Subcellular Localization: Tissue Specificity:
E.coli-derived human CTBP2 recombinant protein (Position: H321-Q445). human CTBP2 shares 99.2% and 98.4% amino acid (aa) sequence identity with mouse and rat CTBP2, respectively.
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml. Background: The E1a region of group C adenoviruses encodes 2 nearly identical proteins that are largely responsible for the oncogenic properties of adenoviruses. The CTBP1 protein binds to the C-terminal half of these E1A proteins. It's predicted that CTBP2 is a 445-amino acid protein and it is 72% identical to CTBP1. The CTBP2 gene is mapped to chromosome 10q26.13. CTBP2 is a mammalian corepressor that targets diverse transcriptional regulators. It bounds the short medial portion of delta-EF1 containing the PLDLSL motif and it enhances transrepression activity of delta-EF1. Subcellular Localization: Tissue Specificity:
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml. Background: Desmoglein-2 is a protein that in humans is encoded by the DSG2 gene. These desmoglein gene family members are located in a cluster on chromosome 18. This second family member is expressed in colon, colon carcinoma, and other simple and stratified epithelial-derived cell lines. Mutations in DSG2 display a high degree of penetrance. Disease expression was of variable severity with LV involvement a prominent feature. The low prevalence of classical ECG changes highlights the need to expand current diagnostic criteria to take account of LV disease, childhood disease expression, and incomplete penetrance. Subcellular Localization: Tissue Specificity:
E.coli-derived human Annexin A1 recombinant protein (Position: A2-N346). Human Annexin A1 shares 88% and 89% amino acid (aa) sequence identity with mouse and rat Annexin A1, respectively.
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml. Background: ANXA1, also known as lipocortin I or Annexin A1, is a protein that in humans is encoded by the ANXA1 gene. It is mapped to 9q21.13. ANXA1 belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. ANXA1 protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Lower peptide concentrations possibly found in inflammatory situations elicit Ca(2+) transients without fully activating the mitogen-activated protein kinase pathway. This causes a specific inhibition of the transendothelial migration of neutrophils and a desensitization of neutrophils toward a chemoattractant challenge. These findings identified ANXA1 peptides as novel, endogenous FPR ligands and established a mechanistic basis of ANXA1-mediated antiinflammatory effects. Subcellular Localization: Tissue Specificity:
E.coli-derived human PARK7 recombinant protein (Position: A2-D189). Human PARK7 shares 91% amino acid (aa) sequence identity with both mouse and rat PARK7.
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml. Background: Parkinson disease (autosomal recessive, early onset) 7, also known as DJ1, is a protein which in humans is encoded by the PARK7 gene. PARK7 belongs to the peptidase C56 family of proteins. PARK7 is mapped to chromosome 1p36. It acts as a positive regulator of androgen receptor-dependent transcription. It is also involved in tumorigenesis and in maintaining mitochondrial homeostasis. This gene may also function as a redox-sensitive chaperone, as a sensor foroxidative stress, and it apparently protects neurons against oxidative stress and cell death. It has been found that PARK7 mutations that impair transcriptional coactivator function can render dopaminergic neurons vulnerable to apoptosis and may contribute to the pathogenesis of Parkinson disease. Subcellular Localization: Tissue Specificity:
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml. Background: Aconitase 2, mitochondrial is a protein that in humans is encoded by the ACO2 gene. The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification. Subcellular Localization: Tissue Specificity:
E.coli-derived human gamma Catenin recombinant protein (Position: M556-A745). Human gamma Catenin shares 98% amino acid (aa) sequence identity with both mouse and rat gamma Catenin.
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml. Background: Junction plakoglobin(JUP), also known as gamma-catenin, is a protein that in humans is encoded by the JUP gene. It is a member of the catenin protein family and homologous to ?-catenin, and it is mapped to 17q21.2. This gene encodes a major cytoplasmic protein that is the only known constituent common to submembranous plaques of both desmosomes and intermediate junctions. This protein forms distinct complexes with cadherins and desmosomal cadherins. Meanwhile, JUP may have distinct roles in Wnt signaling and cancer via differential effects on downstream target genes. Subcellular Localization: Tissue Specificity:
E.coli-derived human LYRIC recombinant protein (Position: D101-Q270). Human LYRIC shares 94% amino acid (aa) sequence identity with both mouse and rat LYRIC.
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml. Background: MTDH (Metadherin), also known as protein LYRIC or astrocyte elevated gene-1 protein (AEG-1) is a protein that in humans is encoded by the MTDH gene. AEG-1 is involved in HIF-1alpha mediated angiogenesis. AEG-1 also interacts with SND1 and involved in RNA-induced silencing complex (RISC) and plays very important role in RISC and miRNA functions. AEG-1 induces an oncogene called Late SV40 factor (LSF/TFCP2) which is involved in thymidylate synthase (TS) induction and DNA biosynthesis synthesis. Late SV40 factor (LSF/TFCP2) enhances angiogenesis by transcriptionally up-regulating matrix metalloproteinase-9 (MMP9). AEG-1 acts as an oncogene in melanoma, malignant glioma, breast cancer and hepatocellular carcinoma. It is highly expressed in these cancers and helps in progression and development of these cancers. It is induced by c-Myc oncogene and plays very important role in anchorage independent growth of cancer cells. Subcellular Localization: Tissue Specificity:
E.coli-derived human MDH2 recombinant protein (Position: A9-L223). Human MDH2 shares 97.7% and 98.1% amino acid (aa) sequence identity with mouse and rat MDH2, respectively.
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml. Background: Malate dehydrogenase, mitochondrial also known as malate dehydrogenase 2 is an enzyme that in humans is encoded by the MDH2 gene. Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Several transcript variants encoding different isoforms have been found for this gene. Subcellular Localization: Tissue Specificity:
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml. Background: Calnexin (CNX) is a 67 kDa integral protein of the endoplasmic reticulum. This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding different isoforms have been described. Subcellular Localization: Tissue Specificity:
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml. Background: Activated RNA polymerase II transcriptional coactivator p15, also known as positive cofactor 4 (PC4) or SUB1 homolog, is a protein that in humans is encoded by the SUB1 gene. This gene is mapped to 5p13.3. The transcriptional cofactor PC4 is an ancient single-strand DNA (ssDNA)-binding protein that has a homologue in bacteriophage T5 where it is likely the elusive replicative ssDNA-binding protein. The recombinant PC4 is shown to function identically to the native protein through its interaction with TAFs. Subcellular Localization: Tissue Specificity:
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml. Background: Claudin 3, also known as CLDN3, is a protein which in humans is encoded by the CLDN3 gene. Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this intronless gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is also a low-affinity receptor for Clostridium perfringens enterotoxin, and shares aa sequence similarity with a putative apoptosis-related protein found in rat. Subcellular Localization: Tissue Specificity:
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml. Background: NADP-dependent malic enzyme is a protein that in humans is encoded by the ME1 gene. This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. Subcellular Localization: Tissue Specificity:
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml. Background: Cleavage and polyadenylation specificity factor subunit 6 is a protein that in humans is encoded by the CPSF6 gene. The protein encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. The cleavage factor complex is composed of four polypeptides. This gene encodes the 68kD subunit. It has a domain organization reminiscent of spliceosomal proteins. Subcellular Localization: Tissue Specificity:
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml. Background: Solute carrier family 12(sodium/potassium/chloride transporters), member 2, also known as NKCC1, is widely distributed throughout the body, especially in organs that secrete fluids, called exocrine glands. By fluorescence in situ hybridization, this gene is mapped to chromosome 5q23.3. The protein encoded by this gene mediates sodium and chloride transport and reabsorption. The encoded protein is a membrane protein and is important in maintaining proper ionic balance and cell volume. This protein is phosphorylated in response to DNA damage. Three transcript variants encoding two different isoforms have been found for this gene. Subcellular Localization: Tissue Specificity:
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml. Background: ZEB1 (Zinc Finger E Box-Binding Homeobox 1), also called TCF8, NIL2A or DELTA-EF1, is a protein that in humans is encoded by the ZEB1 gene. Fluorescence in situ hybridization localized the ZEB1 gene to chromosome 10p11.2. Krafchak et al. (2005) demonstrated a complex (core plus secondary) binding site for TCF8 in the promoter of the COL4A3 gene, mutant in Alport syndrome and which encodes collagen type IV alpha-3. They detected expression of TCF8 in cornea. Nishimura et al. (2006) found that delta-Ef1 was upregulated during differentiation in a mouse smooth muscle cell (SMC) line. Subcellular Localization: Tissue Specificity:
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml. Background: ILK, also known as Integrin-linked kinase, is a serine-threonine protein kinase. Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK) interacts with the cytoplasmic domain of beta-1 integrin. This gene was initially described to encode a serine/ threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. Recent results showed that ILK contains 5 ankyrin-like repeats, and that the C-terminal kinase domain is actually a pseudo-kinase with adaptor function. Subcellular Localization: Tissue Specificity:
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml. Background: Glycine decarboxylase also known as glycine cleavage system P protein or glycine dehydrogenase is an enzyme that in humans is encoded by the GLDC gene. Degradation of glycine is brought about by the glycine cleavage system, which is composed of four mitochondrial protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase), H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase). The protein encoded by this gene is the P protein, which binds to glycine and enables the methylamine group from glycine to be transferred to the T protein. Defects in this gene are a cause of nonketotic hyperglycinemia (NKH). Subcellular Localization: Tissue Specificity:
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml. Background: WD repeat-containing protein 1 is a protein that in humans is encoded by the WDR1 gene. It is mapped to 4p16.1. This gene encodes a protein containing 9 WD repeats. WD repeats are approximately 30- to 40-amino acid domains containing several conserved residues, mostly including a trp-asp at the C-terminal end. WD domains are involved in protein-protein interactions. The encoded protein may help induce the disassembly of actin filaments. Two transcript variants encoding different isoforms have been found for this gene. Subcellular Localization: Tissue Specificity:
A synthetic peptide corresponding to a sequence at the C-terminus of human Ataxin 1, different from the related mouse and rat sequences by one amino acid.
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml. Background: Ataxin-1 is a protein that in humans is encoded by the ATXN1 gene. The ATXN1 gene had been mapped to 6p23 by in situ hybridization. Ataxin-1 (ATXN1), a causative factor for spinocerebellar ataxia type 1 (SCA1), and the related Brother of ATXN1 (BOAT1) are human proteins involved in transcriptional repression. ATXN1 and BOAT1 might participate in several Notch-controlled developmental and pathological processes. Subcellular Localization: Tissue Specificity:
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml. Background: HLA class II histocompatibility antigen, DR alpha chainis aproteinthat in humans is encoded by the HLA-DRAgene. It is mapped to 6p21.32. HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5. Subcellular Localization: Tissue Specificity:
Hsp90 (heat shock protein 90) is one of the most abundant chaperones in the cytosol of eukaryotic cells. It interacts with various proteins, including protein kinases and transcription factors, and either facilitates their stabilization and activation or directs them for proteasomal degradation. Hsp90 thus affects multiple signaling pathways and biological processes and modulation of this single target offers the prospect of simultaneous intervence to various key points of oncogenic transformation. Hsp90 operates as a dimer in a conformational cycle driven by ATP binding and hydrolysis. There are two isoforms, alpha and beta, of vertebrate Hsp90. Whereas Hsp90 beta is expressed constitutively to a high level, Hsp90 alpha is stress-inducible and is overexpressed in many cancerous cells.SpecificityThe antibody MBH90B recognizes the EEVHHG epitope within the N-terminal part of Hsp90 beta an ubiquitously expressed intracellular protein with calculated Mw of 83.3 kDa, however, migrating as a 90 kDa band under reducing SDS-PAGE conditions.Application detailsWestern blotting: Recommended dilution: 1 ?g/ml.
Human leukocyte antigen G (HLA-G), belonging to MHC class I glycoproteins, plays important roles in both physiological and pathological immunotolerance. It gives an inhibitory signal to cytotoxic T cells, NK cells, monocytes, and some other immune cells. It also induces regulatory T cells and anti-inflammatory macrophages. HLA-G is important e.g. for maternal tolerance to the fetus, and for immunomodulation in particular adult tissues, such as in cornea, pancreatic islets, thymus and other. On the other hand, it is expressed in many solid and hematologic malignancies, where it contributes to evasion of the immune surveillance. HLA-G expression pattern in cancer is an important prognostic factor regarding a poor clinical outcome. Unlike most other MHC glycoproteins, HLA-G acts as an immune checkpoint molecule rather than as an antigen presenting molecule. It concerns both transmembrane and soluble HLA-G isoforms. Among other, HLA-G can promote Th2 immunological response and downregulate Th1 immunological response. For its benefits regarding allograft tolerance, including embryo implantation, soluble HLA-G (sHLA-G) can be used as a marker of developmental potential of embryos during the process of in vitro fertilization. Similarly, sHLA-G concentrations in maternal serum are decreased in preeclampsia. Transplanted patients with increased sHLA-G serum levels have improved allograft acceptance. On the other hand, increased sHLA-G can also indicate presence of malignant (sometimes also of benign) tumor cells. Another important topic is induction of HLA-G expression (sometimes associated with shedding of HLA-G from the cell surface) by some anti-cancer or anti-viral therapies, which can weaken the therapy effect. Monitoring of HLA-G in patients thus has a wide usage.SpecificityThe antibody MBH90AB recognizes the epitope EEEVE within N-terminal part of ubiquitously expressed Hsp90 alpha and Hsp90 beta intracellular proteins with calculated Mw of 84.7 kDa and 83.3 kDa, respectively, however, migrating as 90 kDa bands under reducing SDS-PAGE conditions.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml. Intracellular staining. <br>Immunohistochemistry (frozen sections): Recommended dilution: 10 ?g/ml; positive tissue: placenta.<br>Immunohistochemistry (paraffin sections): Recommended dilution: 10 ?g/ml; positive tissue: placenta. <br>ELISA: Positive control: HeLa/HLA-G5 transfectants cell lysate, HeLa/HLA-G5 cell supernatant; negative control: HeLa cell lysate. The antibody 5A6G7 has been tested as the capture antibody in a sandwich ELISA for analysis of soluble HLA-G in combination with antibody W6/32 (cat. no. 1B-422-C100). <br>Western blotting: Positive control: JEG-3 cell lysate, reducing conditions, 12% AA SDS-PAGE.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
5A6G7
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
HLA-F, an MHC class I molecule, is a type I transmembrane protein (heavy chain), which forms heterodimers with beta-2 microglobulin (light chain) and binds to KIR3DS1, KIR3DS4, KIR3DL2, ILT2, ILT4, and TAP. Unlike most other HLA proteins, HLA-F is mainly localized in the endoplasmic reticulum and Golgi apparatus, with only a small amount present on the cell surface in some cell types; surface expression can be induced by cell activation. It is thought to bind a restricted subset of peptides for immune presentation. Multiple transcript variants encoding different isoforms have been found for HLA-F gene. These variants lack a coding exon found in transcripts from other HLA paralogues due to an altered splice acceptor site, resulting in a shorter cytoplasmic domain.SpecificityThe mouse monoclonal antibody 5A6G7 was generated to a peptide corresponding to C-intron 4-encoded sequence. This antibody does not crossreact with the full-length HLA-G1 isoform and thus allows to distinguish between secreted HLA-G5 and HLA-G6 isoforms from shedded HLA-G1.Application detailsFlow cytometry: Recommended dilution: 1-5 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
3D11
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
HLA-E (human leukocyte antigen E) is a non-classical MHC I antigen, which is important for dialogue with NK cells and their regulation through interaction with CD94/NKG2 receptor. Like other MHC I molecules, transmembrane HLA-E molecule (45 kDa) associates with beta2 microglobulin. Unlike HLA-G, expression of HLA-E molecules is not so restricted, but it has been detected at least at mRNA level in virtually all cells and tissues examined. In peripheral blood, HLA-E protein is expressed at least in all mononuclear cells, but in different quantity (B cells and monocytes more than T cells and NK cells).SpecificityThe mouse monoclonal antibody 3D11 recognizes an extracellular epitope of HLA-F, a 42 kDa type I transbembrane protein expressed on B cells, NK cells, monocytes, and T cells, but mainly in the endoplasmic reticulum and Golgi apparatus, only a small amount on the cell surface, where, however, it can be expressed after cell activation.Application detailsImmunohistochemistry (paraffin sections): Recommended dilution: 10 ?g/ml; positive tissue: spleen. <br>Flow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-E/07
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
SpecificityThe antibody MEM-E/07 recognizes an extracellular epitope on native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells.
The published results revealed that the antibody cross-reacts with some classical MHC Class I molecules (MHC Class Ia): HLA-B7 (strongly), HLA-B8 (moderately), HLA-B27, -B44 (weakly).Application detailsWestern blotting: Non-reducing conditions. <br>Flow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
HL-38
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
HLA-DR, a member of MHC class II glycoproteins, that bind intracellularly processed peptides and present them to the Th cells, is composed of 36 kDa alpha chain and 27 kDa beta chain, both anchored in the plasma membrane. Together with other MHC II molecules HLA-DR plays a central role in the immune system.SpecificityThe antibody HL-38 recognizes an extracellular common epitope on beta-chain of human HLA-DR and HLA-DP. DR and DP are the isotypes of human MHC Class II molecules expressed on antigen-presenting cells (APC; dendritic cells, B lymphocytes, monocytes, macrophages).Application detailsFlow cytometry: Recommended dilution: 2 ?g/ml; positive control: B-lymphocytes, monocytes, DAUDI cell line, RAJI cell line. <br>Western blotting and Immunoprecipitation: Positive control: B-lymphocytes, monocytes, DAUDI cell line, RAJI cell line; non-reducing conditions.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-12
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
HLA-C, a member of MHC class I glycoproteins, is one of polymorphysm typing targets, which are important for transplantation. The HLA system plays an important role in the occurrence and outcome of infectious diseases. The structural spike and the nucleocapsid proteins of the novel coronavirus SARS-CoV-2, which causes coronavirus disease 2019 (COVID-19), are reported to contain multiple Class I epitopes with predicted HLA restrictions. Individual HLA genetic variation may help explain different immune responses to a virus across a population. It has been described that HLA-C interacts with human herpesvirus 8 MIR1 protein.SpecificityThe antibody MEM-12 recognizes a common extracellular epitope on human HLA-DR which is dependent on the association of alpha and beta chains. DR is the isotype of human MHC Class II molecules expressed on antigen-presenting cells (APC; dendritic cells, B lymphocytes, monocytes, macrophages).Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
DT-9
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
HLA-B7 allele of human HLA class I major histocompatibility (MHC) antigen indicates higher risk of breast cancer and cervical cancer. Expression of HLA-B7 together with HLA-B27 is associated with increased susceptibility to spondyloarthropaties. Flow cytometry detection of these two alleles is being used to screen for patients, who suffer from inflammatory disorders affecting the sacroiliac and intervertebral joints, such as ankylosing spondylosis (AS). The HLA-B7 antigen (11 alleles) is expressed in 22% of healthy Caucasian individuals.SpecificityThe mouse monoclonal antibody DT-9 recognizes an extracellular epitope on HLA-C member of MHC class I molecules. It does not crossreact with HLA-A or HLA-B allotypes.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
BB7.1
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.SpecificityThe mouse monoclonal antibody BB7.1 recognizes an extracellular antigen of HLA-B7 antigen. Although highly specific, it can cross-react with HLA-B42 antigen.Application detailsFlow cytometry: Recommended dilution: 1 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-81
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Histone deacetylase 6 (HDAC6), like other histone deacetylases, affects gene expression by regulation of chromatin remodeling. HDAC6 contains an internal duplication of two catalytic domains which appear to function independently of each other. Besides histones, HDAC6 deacetylates also other substrates including alpha tubulin and HSP90 alpha, and is involved in protein trafficking and degradation, as well as in affecting of cell shape and migration. Deregulation of HDAC6 expression and activity is associated with many diseases.SpecificityThe antibody MEM-81 reacts with an extracellular epitope on human classical MHC Class I molecules in native cell-surface forms. MHC Class I molecules (MHC Class Ia) are expressed on the surface of human nucleated cell types.Application detailsImmunocytochemistry: Recommended dilution: 5-10 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
2H3
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
GRAP2/GADS (Grb2-related adaptor protein 2 / Grb2-related adaptor downstream of Shc) is a cytoplasmic adaptor protein containing N- and C-terminal SH3 domains flanking a central SH2 domain and a proline/glutamine-rich region. It is expressed predominantly in lymphoid tissue and hematopoietic cells, particularly in T cells. GRAP2/GADS plays a pivotal role during the early events of T cell signal transduction by recruiting the adaptor protein SLP-76 and its associated molecules, such as Vav, Nck, Itk, and ADAP, to the transmembrane adaptor protein LAT. GRAP2/GADS also binds several other signaling proteins, namely Gab2, HPK1 (hematopoietic progenitor kinase 1), and Cbl. Unlike similar adaptor protein Grb2, GRAP2/GADS shows higher selectivity when binding to the particular phosphorylated tyrosines of LAT adaptor.SpecificityThe mouse monoclonal antibody 2H3 recognizes D1 domain of human histone deacetylase 6 (HDAC6; intracellular antigen), amino acids 281-291. Crossreactivity with other species was not tested.Application detailsFlow cytometry: Recommended dilution: 2-6 ?g/ml. Intracellular staining.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
UW40
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Granzyme B is a serine protease that is expressed in cytoplasmic granules of cytotoxic T lymphocytes and NK cells. Vectorial secretion of perforin and granzymes is responsible for their granule-mediated cytotoxicity. Granzyme B plays a pivotal role in the induction of apoptosis in the target cells by activation of caspases. Moreover, granzyme B was reported to cleave directly alpha-tubulin, leading to perturbation of microtubule networks during the induced cell death.SpecificityThe mouse monoclonal antibody UW40 recognizes GRAP2/GADS, a 41 kDa cytoplasmic adaptor protein that plays a pivotal role during the early events of signal transduction in T cells.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml. Intracellular staining.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
CLB-GB11
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
GFAP (glial fibrillary acidic protein) was discovered by Bignami et al. (1972) as a major fibrous protein of multiple sclerosis plaques. It was subsequently found to be a member of the 10 nm or intermediate filament protein family, specifically the intermediate filament protein family class III, which also includes peripherin, desmin and vimentin. GFAP is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. It is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. Although its function is not fully understood, GFAP protein is probably involved in controlling the shape and movement of astrocytes. The protein probably also plays a significant role in the interactions of astrocytes with other cells, which are required for the formation and maintenance of the insulating layer (myelin) that covers nerve cells. Additionally, GFAP protein may assist in maintaining the protective barrier that allows only certain substances to pass between blood vessels and the brain (blood-brain barrier).In adults, GFAP levels increase as a result of the proliferation of astrocytes that occurs in a response to a variety of physical, chemical and etiological insults, including Alzheimer’s disease, epilepsy and multiple sclerosis.Antibodies to GFAP are therefore very useful as markers of astrocytic cells and neural stem cells and for distinguishing of neoplasms of astrocytic origin from other neoplasms in the central nervous system. Finally, Alexander's disease was recently shown to be caused by point mutations in protein coding region of the GFAP gene (Brenner et al., 2001). All forms of Alexander disease are characterized by the presence of Rosenthal fibers, which are GFAP containing cytoplasmic inclusions found in astrocytes.SpecificityThe mouse monoclonal antibody CLB-GB11 recognizes granzyme B, a 31 kDa serine protease expressed intracellularly in activated Tc cells and NK cells.Application detailsWestern blotting: Recommended dilution: 1-2 ?g/ml.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
GF-02
Concentration:
1 mg/ml
Format:
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Storage buffer:
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
The gamma-tubulin (TUBG1; relative molecular weight about 48 kDa) is a minor member of tubulin family (less that 0.01% of tubulin dimer). The gamma-tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing gamma-tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface. Humans possess two gamma-tubulin genes. Gamma-tubulin 1 represents a ubiquitous isotype, whereas gamma-tubulin 2 is found predominantly in the brain, where it may be endowed with divergent functions beyond microtubule nucleation.SpecificityThe mouse monoclonal antibody GF-02 exclusively reacts with intact GFAP molecules. GFAP is the principal marker of astroglial cells in the central nervous system; it is specifically expressed in satellite cells in peripheral ganglia and in non myelinating Schwann cells in peripheral nerves. The GFAP protein runs on gels at ~55 kDa protein, usually associated with lower Mw bands which are thought to be proteolytic fragments and alternate transcripts from the single gene.Application detailsImmunocytochemistry: Recommended dilution: 1-2 ?g/ml. Staining technique: (a) Fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) Fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. Positive control: P-19 murine embryonal carcinoma cell line, 3T3 murine fibroblasts. The antibody TU-30 stains only fixed cells.<br>Western blotting: Recommended dilution 1-2 ?g/ml, reducing conditions.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
TU-30
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
FoxP3 (forkhead box protein 3), a highly conserved forkhead/winged-helix transcription factor, plays a crucial role in maintaining immune homeostasis by governing the development and function of regulatory T cells. It is constitutively expressed at high level in CD25+ CD4+ Treg cells and at low level in a CD25- CD4+ Treg cell subset. Defects in gene encoding FoxP3 protein cause the scurfy phenotype in mice, and in human the IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome.SpecificityThe antibody TU-30 recognizes C-terminus (amino acids 434-449 in human) of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC). The epitope was located in the amino acid sequence TRPDYI (aa439-444 in human), which is present on human gamma-tubulin 1 but not on human gamma-tubulin 2.Application detailsWestern blotting: Recommended dilution: 2 ?g/ml. <br>Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
3G3
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
FOLR2 (folate receptor beta) is a cell surface protein that was originally thought to be specific for placenta, but it can be also expressed in other tissues, including hematopoietic cells. Its expression is increased in malignant tissues. FOLR2 may play a role in the transport of methotrexate in synovial macrophages in rheumatoid arthritis patients. FOLR2 is a marker for macrophages generated in the presence of M-CSF (M2), including M2-like tumor-associated macrophages, which exert potent immunosuppressive functions within the tumor environment, but not GM-CSF (M1), and whose expression correlates with increased folate uptake ability.SpecificityThe mouse monoclonal antibody 3G3 recognizes N-terminal region of FoxP3, a 47-55 kDa transcription factor (intracellular antigen), which is the master regulator in the development and function of regulatory T cells.Application detailsWestern blotting: Non-reducing conditions. <br>Flow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
EM-35
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
The oncoprotein EGFR (epidermal growth factor receptor), also known as HER1 / ErbB1, is a member of ErbB family of cell surface receptor tyrosine kinases. This 170 kDa transmembrane glycoprotein is often associated with cancerogenesis, although its activation state is controlled at various levels including trafficking and degradation steps. Binding of receptor-specific ligands to the EGFR ectodomain results in formation of homodimeric or heterodimeric kinase-active complexes into which HER2 / ErbB2 is recruited as a preferred partner. Subsequent signaling cascades such as RAS/MAPK and PI3K/AKT pathways lead to cell proliferation and survival responses.SpecificityThe mouse monoclonal antibody EM-35 recognizes an extracellular epitope on FOLR2, a 30-40 kDa cell surface protein serving as a receptor for folic acid.Application detailsFlow cytometry: Recommended dilution: 2-6 ?g/ml.
Antibody Isotype:
IgG2b kappa
Monosan Range:
MONOSAN
Clone:
EGFR1
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Drebrin is an actin-binding protein, which is expressed mainly in neurons and plays important role in their morphogenesis. The highest level of its expression is in developing brain. Both in neurons and non-neuronal cells drebrin acts as a key regulator of actin cytoskeleton remodelling, affecting especially intercellular junctions, such as dendritic spines of neurons or the immune synapses of T cells. Decrease of drebrin amount in the brain seems to be associated with Alzheimer's disease and Down syndrome, and in case of B-cell precursor acute lymphoblastic leukemia (BCP-ALL) lower drebrin expression correlates with higher risk of relapse.SpecificityThe mouse monoclonal antibody EGFR1 reacts with extracellular domain of human protein kinase EGFR (ErbB1 / HER1); epitope within amino acids 6-273.Application detailsWestern blotting: Positive control: REH, Nalm-6, HEK293 cells. <br>Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
DBN-N-03
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
DR3, also known as APO-3, TRAMP or TNFRSF25, is a death domain-containing receptor of TNFR family, which is expressed preferentially in peripheral blood leukocytes and in the lymphocyte-enriched tissues. Its expression has been shown to be especially up-regulated in activated T cells. DR3 participates e.g. in the removal of self-reactive T cells in the thymus. The ligand for DR3 is TL1A (TNF-like ligand 1A), which is expressed in a variety of cell types (induced by inflammatory stimuli), and can also be released as a soluble factor. The TL1A/DR3 axis has been shown to costimulate T cells to produce a wide variety of cytokines and leads to T cell differentiation towards Th1 and Th17 types.SpecificityThe mouse monoclonal antibody DBN-N-03 recognizes drebrin, an approximately 100-125 kDa intracellular regulator of actin cytoskeleton.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
JD3
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
DLL4 (Delta-like 4) is one of five ligands of Notch receptors. It interacts with Notch1 and Notch4. DLL4 is up-regulated at sites of physiologic and pathologic angiogenesis, whereas its expression is low in most adult normal tissues. It is also highly expressed in human clear-cell renal carcinomas, bladder cancers, and breast cancers. Blocking the DLL4-Notch interaction seems to be a promissing therapeutic approach.SpecificityThe mouse monoclonal antibody JD3 recognizes an extracellular epitope of DR3 (APO-3, TNFRSF25), a transmembrane protein of TNFR superfamily expressed mainly in lymphocyte-enriched tissues.Application detailsFlow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
MHD4-46
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
DDIT4L (DNA-damage-inducible transcript 4-like), also known as REDD2 (regulated in development and DNA damage response 2) or RTP801L is a stress-inducted protein, which was shown to mediate monocyte cell death through a reduction in thioredoxin-1 expression, and is highly expressed in atherosclerotic lesions. Stimulation of DDIT4L expression in macrophages increases oxidized LDL-induced macrophage death.SpecificityThe mouse monoclonal antibody MHD4-46 recognizes the extracellular domain of DLL4 (Delta-like ligand 4), a type I transmembrane protein which plays an important role in vascular development.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml. Intracellular staining.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
DDIT-03
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Daxx is an apoptosis-modulating death domain-associated protein with functions in transcriptional regulation. Daxx functions both in cytoplasm, where it interacts with Fas, and in nucleus (residing in PML oncogenic domains), where it is involved in SUMO-dependent regulation of transcription and subnuclear compartmentalization. Daxx senzitizes the cells to apoptosis, but on the other hand, this protein may also serve in preventing apoptosis in the early embryo. Even regarding the transcription, Daxx can serve both as a corepressor and a coactivator. During ischemic stress, Daxx translocates from the nucleus to the cytoplasm, where in regulates sodium hydrogen exchanger isoform 1 (NHE1).SpecificityThe mouse monoclonal antibody DDIT-03 recognizes DDIT4L / REDD2 protein, which belongs to intracellular stress-induced proteins involved in mediation of cell death.Application detailsWestern blotting: Recommended dilution: 2 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
DAXX-03
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.SpecificityThe mouse monoclonal antibody DAXX-03 reacts with C-terminal part of Daxx, a 110 kDa intracellular protein.Application detailsFlow cytometry: Recommended dilution: 1-5 ?g/ml. Intracellular staining.<br>Immunohistochemistry: Recommended dilution: 5-10 ?g/ml.<br>Western blotting: Recommended dilution: 1-2 ?g/ml, positive control: MCF-7, BA-17, HT-29 cells.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
A53-B/A2
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratins 10 and 13 both belong to type I family (acidic cytokeratins).SpecificityThe mouse monoclonal antibody A53-B/A2 recognizes the rod domain of cytokeratin 19 (40 kDa), an intracellular antigen constituting intermmediate cytoskeleton filaments. Cytokeratin 19 is not expressed in hepatocytes; it is often co-expressed with cytokeratin 7.Application detailsImmunohistochemistry (paraffin sections): Standard ABC technique (DAB+), recommended dilution: 10 ?g/ml (1:100), positive tissue: tonsil. Pretreatment: 0.1% pepsin in 0.1 M HCl for 30 min at room temperature or Target Retrieval Solution - High pH. The antibody DE-K13 reacts exclusively with Cytokeratin 13. <br>Immunohistochemistry (frozen sections): Use at an assay dependent dilution. <br>Western blotting: recommended dilution: 1-3 ?g/ml.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
DE-K13
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.SpecificityThe mouse monoclonal antibody DE-K13 reacts with cytokeratin 10 (56.5 kDa) and cytokeratin 13 (53 kDa). Cytokeratins are members of intermediate filaments subfamily of intracellular proteins, represented in epithelial tissues. DE-K13 recognizes only cytokeratin 13 on formalin-fixed, paraffin-embedded tissue sections.Application detailsImmunohistochemistry (paraffin sections): Recommended dilution: 10 ?g/ml; positive tissue: skin, pretreatment: trypsin/temperature 37 °C.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
VIK-10
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Cyclin D1 (PRAD1, Bcl-1) is a cytoplasmic and nuclear protein, which is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Whereas during G1 phase cyclin D1 accumulates in the nucleus, it translocates into the cytoplasm during S phase. Without growth factor-mediated stimulation, cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest.SpecificityThe antibody VIK-10 reacts with cytokeratin 10 (56.5 kDa; intracellular antigen). Cytokeratins are members of intermediate filaments subfamily represented in epithelial tissues.Application detailsImmunohistochemistry: Recommended dilution: 5-10 ?g/ml. <br>Flow cytometry: Recommended dilution: 3-12 µg/ml. Intracellular staining.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
DCS-6
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
KLH-conjugated peptide corresponding to the amino acids 160-173 of human CLIC5a (Cys-Ile-Asp-Ala-Asn-Thr-Ser-Asp-Lys-Gly-Ser-Arg-Arg coupled with KLH).
CLIC5a belongs to the family of chloride intracellular channel (CLIC) proteins, all sharing a highly conserved C terminus and variable N terminus. Human CLIC5 is transcribed in two isoforms, 32 kDa CLIC5a (251 amino acids) and 49 kDa CLIC5b (410 amino acids). These proteins exist in a soluble form and their function as ion channels in vitro has not been fully confirmed in vivo. CLIC5a is a component of complexes of actin, ezrin, and several other actin-associated proteins and is important for functionality of actin-based structures.SpecificityThe mouse monoclonal antibody DCS-6 recognizes cyclin D1, an ubiquitously expressed 33 kDa intracellular protein that migrates as a 36 kDa band under reducing SDS-PAGE conditions.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml. Intracellular staining.
Antibody Isotype:
IgG2b kappa
Monosan Range:
MONOSAN
Clone:
CLIC5-02
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clathrin is a submembrane protein that polymerizes into coat-like lattices, which results in membrane invagination. The basic oligomers are composed of three clathrin heavy chain (180 kDa) and three light chain (30 kDa) subunits and the process of polymerization is dynamically regulated by the light chains. Interaction of clathrin with the plasma membrane is mediated by adaptor proteins (AP1-4) specific for different cellular compartments. Another proteins, such as endophilin, epsin and amphiphysin are involved in membrane invagination and clathrin rearrangements. Finally, dynamin functions at the fission stage of clathrin-mediated endocytosis.SpecificityMouse monoclonal antibody CLIC5-02 recognizes CLIC5a, a 32 kDa intracellular protein which associates with proteins of actin complexes. Crossreactivity with CLIC5b was not determined.Application detailsFlow cytometry: Recommended dilution: 2-5 ?g/ml; positive control: human blood leukocytes. Intracellular staining.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
BF-06
Concentration:
1 mg/ml
Format:
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Storage buffer:
Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
CD370 / CLEC9A, also known as DNGR1, is a type II transmembrane glycoprotein with extracellular C-type lectin domain and intracellular ITAM-containing domain. Its expression is restricted to BDCA3+ conventional dendritic cells and to a subset of CD14+ CD16- monocytes. CD370 serves as a receptor for ubiquitous preformed acid-labile protein associated ligands that are exposed when the cell membrane is damaged, such as on necrotic cells. Its triggering by these ligands mediates recruitment and activation of the tyrosine kinase Syk and leads to their cross-presentation to the immune system.SpecificityThe antibody BF-06 recognizes clathrin heavy chain, an ubiquitously expressed 180 kDa intracellular protein involved in receptor-mediated endocytosis.Application detailsFlow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
8F9
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD325 (N-cadherin) is a type I transmembrane protein, which forms a complex with catenins, that is linked to the actin cytoskeleton. This complex is important in synapses and for functional plasticity of neurons, and is also essential for embryonic development. Decreased CD325 cleavage caused by mutations in presenilin 1 is associated with Alzheimer´s disease. Besides nervous system, CD325 is expressed on the surface of malignant T cells, and increases their adhesion to epithelia, as well as their ability to invade and metastasize to inflammatory sites.SpecificityThe mouse monoclonal antibody 8F9 recognizes an extracellular epitope of CD370 / CLEC9A (DNGR1), a type II transmembrane protein functioning as an endocytic receptor on BDCA31+ dendritic cells and on a subset of CD14+ CD16- monocytes.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
8C11
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence.SpecificityThe mouse monoclonal antibody 8C11 recognizes an extracellular epitope of CD325 (N-cadherin), a transmembrane protein associated with actin cytoskeleton system, which is expressed mainly by neurons, osteoblasts, endothelial cells, and stem cells.Application detailsFlow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
IVA26
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1).SpecificityThe mouse monoclonal antibody IVA26 recognizes an extracellular epitope on MHC class I (alpha + beta2m chains) expressed in various animals.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
IVA94
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD41 (platelet glycoprotein IIb, integrin alpha IIb) is composed of two subunits (120 kDa transmembrane alpha chain and 23 kDa extracellular beta chain) and interacts with CD61 (platelet glycoprotein IIIa, integrin beta 3) in the presence of calcium to form a functional adhesive protein receptor. CD41/CD61 complex is one of the earliest markers of the megakaryocytic lineage. Upon blood vessel damage, this receptor binds to a variety of proteins including von Willebrand factor, fibrinogen, fibronectin and vitronectin, and it is involved in platelet aggregation.SpecificityThe antibody IVA94 reacts with an extracellular epitope of CD62L antigen (bovine). CD62L (L-selectin) is a 74-95 kDa single chain type I glycoprotein expressed on most peripheral blood B lymphocytes, T lymphocytes, monocytes and granulocytes; it is also present on a subset of NK cells and certain hematopoietic malignant cells.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG2
Monosan Range:
MONOSAN
Clone:
IVA30
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
SpecificityThe antibody IVA30 reacts with an extracellular epitope on CD41/CD61 complex (bovine), one of the earliest markers of the megakaryocytic lineage. CD41/CD61 complex acts as the receptor for fibrinogen, fibronectin, Von Willebrand factor, vitronectin, thrombin and mediates platelets aggregation. CD41/CD61 is expressed only by platelets and megakaryocytes; the complex may be absent or strongly reduced in Glanzmann's thrombasthenia (GT). This antibody crossreacts with human platelets.Application detailsImmunohistochemistry (paraffin sections): Recommended dilution: 5-10 ?g/ml; positive control: colorectal adenocarcinoma; heat mediated antigen retrieval (sodium citrate); mAb incubation: 1 hour / RT; detection DAB.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
2-25LE
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
SpecificityThe antibody 2-25LE reacts with Lewis<sup>b</sup> blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, detected on the surface of erythrocytes, certain epithelial cells, and in secretions of certain individuals.Application detailsImmunohistochemistry (paraffin sections): Recommended dilution: 5 ?g/ml; positive control: colorectal adenocarcinoma; heat mediated antigen retrieval (sodium citrate); mAb incubation 1 hour / RT, detection DAB.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
7LE
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
SpecificityThe mouse monoclonal antibody 7LE recognizes Lewis<sup>a</sup> blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, expressed on colonic epithelial cells. Lewis<sup>a</sup> may be useful for detection of gastrointestinal, pancreatic and colorectal tumors.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
HE-10
Concentration:
1 mg/ml
Format:
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Storage buffer:
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Human blood group A antigen belongs to a group of carbohydrate determinats carried on both glycolipids and glycoproteins; it is detected on erythrocytes and certain epithelial cells.SpecificityThe mouse monoclonal antibody HE-10 agglutinates erythrocytes of group A, and is excellent as a tumour marker in patients of blood group B and 0. It does not agglutinate erythrocytes of group B and 0. Study with specific oligosaccharides showed that the antibody HE-10 reacts with A and H antigens with chain types 3 and 4 and it does not react with A disaccharide, A trisaccharide, A type 1, A type 2, ALe<sup>b</sup>. The antibody HE-10 does not react with normal tissue sections of donors with blood group B and 0 but it reacts specifically with malignant tissues.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
HE-195
Concentration:
1 mg/ml
Format:
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Storage buffer:
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity; highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs). The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening – this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains.
The beta-tubulin (relative molecular weight around 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer. It is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain.SpecificityThe antibody HE-195 recognizes human blood group A including weak variants A<sub>3</sub>, A<sub>X</sub>, A<sub>3</sub>B, A<sub>X</sub>B. The specifity of antibody HE-195 was confirmed by comparison of specifity and reactivity to standard reagent using >5.000 samples of blood.Application detailsImmunocytochemistry: Recommended dilution: 2-5 ?g/ml, fixed and permeabilized cells.<br>Western blotting: Recommended dilution: 1-2 ?g/ml, reducing conditions.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
TU-12
Concentration:
1 mg/ml
Format:
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Storage buffer:
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
SpecificityThe antibody TU-12 recognizes an epitope located within aa 345-430 of C-terminal domain of beta-tubulin in various species.Application detailsImmunohistochemistry (paraffin sections): Recommended dilution: 10 ?g/ml; standard ABC technique (DAB+), heat retrieval (microwave oven), incubation: overnight at 4°C; positive tissue: human pituitary gland.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
B31.15
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Beta-catenin is a multifunctional protein involved both in cell adhesion and in activation of transcription. Calcium-dependent intercellular adhesion transmembrane glycoprotein E-cadherin interacts by its cytoplasmic domain with reciprocally bound alpha, beta and gamma catenin. Beta-catenin links this complex through alpha-actinin to the cytoskeleton. Functional cadherin-catenin system is important for invasiveness of tumour cells. Beta-catenin level in cytoplasm is controlled by glycogen synthase kinase-3 beta. When activity of this kinase is blocked (e.g. by excessive stimulation of Wnt signaling pathway), hypophosphorylated stable form of beta-catenin accumulates in the cytoplasm, translocates to the nucleus and activates transcription of genes including those that are involved in cell cycle control. As a result, cell division and neoplastic transformation are promoted.SpecificityThe mouse monoclonal antibody B31.15 reacts with human beta Endorphin, an endogenous opiate derived from ACTH gene. ACTH (Corticotropin; human 39 aa) is synthesized by the anterior pituitary gland and stimulates the adrenal cortex; 6 hormones are derived from one ACTH gene: ACTH, lipotropin, alpha-MSH, beta-MSH, endorphin, and one other.Application detailsImmunocytochemistry: Positive control: HT29 human colon adenocarcinoma cell line. <br>Western blotting: Positive control: HT29 human colon adenocarcinoma cell line, FHC human cell line, DLD1 human colon adenocarcinoma cell line, KW1 murine cell line, C57MG murine cell line, 3T3 murine fibroblast cell line. <br>Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
EM-22
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.SpecificityThe mouse monoclonal antibody EM-22 reacts with C-terminal part of beta-catenin (intracellular antigen), an 88 kDa multifunctional protein involved both in cell adhesion and in activation of transcription.Application detailsWestern blotting: Recommended dilution: 1-2 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
AE3
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
ARHGEF4 (Rho guanine nucleotide exchange factor 4), also known as ASEF 1 (adenomatous polyposis coli – stimulated guanine nucleotide exchange factor 1) is an approximately 80 kDa cytoplasmic protein important for growth factor-mediated regulation of cell morphology and migration. Besides N-terminal adenomatous polyposis coli (APC)-binding region (ABR) it contains Dbl homology (DH), pleckstrin homology (PH) and SH3 domains. The SH3 domain inhibits GEF activity of ARHGEF4 by intramolecular interaction with the DH domain, whereas binding of APC stimulates the GEF activity. Activated ARHGEF4 stimulates the small GTPase Cdc42, which leads to decreased cell-cell adherence and enhanced cell migration.SpecificityMouse monoclonal antibody AE3 recognizes all basic type cytokeratins (intracellular antigens), namely K1-K8 (58-67 kDa). This antibody reacts with all layers of epidermis (basal layer and above) and is an excellent marker for distinguishing carcinomas from non-epithelial tumours.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml. Intracellular staining.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
ARHGEF-08
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
ARAP1 (angiotensin II type 1 receptor-associated protein) is an adaptor protein with ArfGAP and RhoGAP activities, containing five PH domains and ankyrin repeate. This adaptor seems to serve as a link between phosphoinositide- Arf-, and Rho-mediated cell signaling. ARAP1 supports the plasma membrane recycling of angiotensin II type 1 receptor (AT1) and is important also for cell-specific trafficking of pro-death receptor TRAIL-R1 (DR4) to the plasma membrane, thus promoting the TRAIL-induced apoptosis in certain cell types. ARAP1 also affects cell spreading.SpecificityThe mouse monoclonal antibody ARHGEF-08 recognizes human intracellular protein ARHGEF4 / ASEF1, approx. 80 kDa guanine nucleotide exchange factor specific for Rac1 and Cdc42. The epitope is located in the rough at the region of SH3 domain.Application detailsWestern blotting: Recommended dilution: 2 ?g/ml
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
ARAP1-2
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity; highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs). The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening – this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains.
The alpha-tubulin (relative molecular weight around 50 kDa) is globular protein that exists in cells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. In different species it is coded by multiple tubulin genes that form tubulin classes (in human 6 genes). Expressed tubulin genes are named tubulin isotypes. Some of the tubulin isotypes are expressed ubiquitously, while some have more restricted tissue expression. Alpha-tubulin is also subject of numerous post-translational modifications. Tubulin isotypes and their posttranslational modifications are responsible for multiple tubulin charge variants - tubulin isoforms. Heterogeneity of alpha-tubulin is concentrated in C-terminal structural domain.
The beta-tubulin (relative molecular weight around 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain.SpecificityThe antibody ARAP1-2 reacts with C-terminal part of ARAP1 (intracellular epitope), a 160 kDa adaptor protein.Application detailsWestern blotting: Recommended dilution: 2 ?g/ml; positive control: porcine brain lysate.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
TU-08
Concentration:
1 mg/ml
Format:
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Storage buffer:
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
AHNAK1 (Desmoyokin) is a large (700 kDa) scaffold protein that translocates to the plasma membrane after an increas of extracellular calcium level or upon proteinkinase C activation and regulates extracellular calcium influx mediated by L-type Ca2+ channels. AHNAK1 has been implicated in diverse signal transduction proceses affecting cell differentiation and proliferation. In response to calcium-dependent intercellular contacts AHNAK1 forms multimeric complexes in the plasma membrane, connected with actin and annexin 2/S100A10 assemblies and is thus involved in organization of the plasma membrane architecture. In epithelial cells, AHNAK1 is localized in cytoplasm or is membrane-associated, but in cells of nonepithelial origin AHNAK1 is predominantly nuclear; it has a weak DNA-binding activity and associates with the DNA-ligase IV-XRCC4 complex.SpecificityThe antibody TU-08 recognizes alpha, beta-tubulin heterodimer (porcine brain), a basic intracellular structural unit of microtubules. Alpha- and beta- tubulins form approximately 100 kDa tubulin heterodimer, a globular protein that polymerizes to form microtubules.Application detailsImmunocytochemistry: Positive control: HeLa human cervix carcinoma cell line, permeabilization is required. <br>Western blotting: Positive control: HeLa human cervix carcinoma cell line.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
EM-09
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
AGR3 (Anterior Gradient 3) protein, also known as AG3 (hAG-3, HAG3 in human), or BCMP11, is a secreted cytoplasmic protein which is involved in metastasis induction and p53 tumour supressor inhibition. It may serve as molecular marker and potential therapeutic target for hormone-responsive breast tumours. Its Xenopus homolog is associated with anteroposterior fate determination during early development.SpecificityThe mouse monoclonal antibody EM-09 reacts with AHNAK1, a 700 kDa multi-functional adaptor protein expressed mainly in epithelial cell, various types of muscle cells and immune cells.Application detailsImmunohistochemistry (paraffin sections): Recommended dilution: 1 ?g/ml; positive tissue: breast cancer. <br>Immunohistochemistry (frozen sections): Recommended dilution: 1 ?g/ml; positive tissue: breast cancer. <br>Western blotting: Recommended dilution: 1 ?g/ml; positive control: T47D breast cancer cell line, negative control: H1299 lung carcinoma cell line. <br>Immunocytochemistry: Recommended dilution: 1 ?g/ml; positive control: T47D breast cancer cell line, negative control: H1299 lung carcinoma cell line.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
AGR3.1
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
AGR2 (Anterior Gradient 2), also known as AG2 (hAG-2, HAG2 in human), or GOB-4, and AGR3 (Anterior Gradient 3), also known as AG3 (hAG-3, HAG3 in human), or BCMP11, are secreted cytoplasmic proteins which are involved in metastasis induction and p53 tumour supressor inhibition. They may serve as molecular markers and potential therapeutic targets for hormone-responsive breast tumours; AGR2 was reported also as a marker of other carcinomas. Xenopus homolog of these proteins is associated with anteroposterior fate determination during early development.SpecificityThe antibody AGR3.1 recognizes the epitope HETTDKNLS within the AGR3 (AG3) protein (19-20 kDa); a secreted cytoplasmic protein which can serve as a marker of carcinogenesis.Application detailsImmunohistochemistry (paraffin sections): Recommended dilution: 5 ?g/ml; Positive tissue: human colon. <br>Western blotting: Recommended dilution: 1 ?g/ml; positive control: T47D breast cancer cell line, negative control: H1299 lung carcinoma cell line. <br>Immunocytochemistry: Recommended dilution: 1 ?g/ml; positive control: T47D breast cancer cell line, negative control: H1299 lung carcinoma cell line.
AGPS (alkylglycerone phosphate synthase), is an enzyme that catalyzes the second step of ether lipid biosynthesis in which acyl-dihydroxyacetone phosphate (acyl-DHAP) is converted to alkyl-DHAP by addition of a long chain alcohol and removal of a long-chain acid anion. The protein is localized to the inner side of the peroxisomal membrane and requires FAD as a cofactor. Mutations in AGPS gene have been associated with type 3 of rhizomelic chondrodysplasia punctata (RCDP3), and Zellweger syndrome. Higher expression of AGPS was observed in BCR/ABL positive leukemias and it was also described to be associated with higher risk of relapse.SpecificityThe antibody AGR3.4 recognizes the PLMII epitope of AGR3 (AG3) and AGR2 (AG2) proteins (19-20 kDa); secreted cytoplasmic proteins which can serve as markers of carcinogenesis.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml. Intracellular staining.<br>Western blotting: Recommended dilution: 1-2 ?g/ml.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
AGPS-03
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Actin is a highly conserved ubiquitous globular protein (G-actin) that polymerizes to form fibrous F-actin microfilaments. In higher eucaryotes several actin isoforms have been identified, that fall into three classes. Alpha actin is a structural component of the contractile apparatus of muscle cells or muscle-derived cells. Beta actin and gamma actin play roles in regulation of cell motility in other cell types. Specific subcellular structures such as stress fibers, focal adhesions, filopodia etc., are formed by involvement of actin cytoskeleton.SpecificityThe mouse monoclonal antibody AGPS-03 recognizes AGPS (alkykglycerone phosphate synthase), an intracellular peroxisomal enzyme important for lipid biosynthesis.Application detailsWestern blotting: Recommended dilution: 1 ?g/ml; positive control: murine femoral muscle, murine heart, negative control: HUVEC line; reducing conditions. <br>Immunohistochemistry (paraffin sections): Antigen retrieval steps generally not required, but e.g. in case of arterial smooth muscle cells or myoepithelial cells, pepsin or trypsin pretreatment is recommended.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
HHF35
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. CThey are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.SpecificityThe mouse monoclonal antibody HHF35 recognizes muscle-specific alpha and gamma actin (42 kDa) in various species. This antibody stains skeletal, smooth and myocardial cells as well as myoepithelial cells and pericytes of small vessels. It is a widely used marker of muscle and muscle-derived cells.Application detailsFlow cytometry: Recommended dilution: 1-5 ?g/ml. Intracellular staining.<br>Western blotting: Recommended dilution: 1-2 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
AE1
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
ABRA1 (Abraxas), also known as CCDC98, is an adaptor protein that is essential for formation and function of BRCA1 A tumor suppressor complex. This complex plays critical roles in DNA repair, cell cycle checkpoint control, and maintenance of genomic stability. ABRA1 mediates interaction of ubiquitin-interacting motif-containing protein RAP80 and deubiquitination enzyme BRCC36 with BRCA1/BARD1. ABRA1 controls both DNA-damage-induced formation of BRCA1 foci and BRCA1-dependent G2/M checkpoint activation.SpecificityMouse monoclonal antibody AE1 recognizes acidic type cytokeratins (intracellular antigens), namely K10, 14, 15, 16, 19 (40-56 kDa). This antibody stains well the basal layer of epidermis and most epithelia.Application detailsFlow cytometry: Recommended dilution: 1-2 ?g/ml. Intracellular staining.<br>Western blotting: Reducing conditions.
Antibody Isotype:
IgG
Monosan Range:
MONOSAN
Clone:
ABRA1-01
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD89 (Fc-alpha-R) is a type I transmembrane glycoprotein serving as a receptor for IgA. Soluble CD89 is detectable in serum and retains its IgA binding capacity. For signal transduction the association with FcR gamma chain homodimers is needed. CD89 is expressed on granulocytes, monocytes, macrophages, dendritic cells and myeloid cell lines. Its expression is upregulated in presence of IgA immune complexes, stimulators (such as LPS, PMA), TNF alpha, IL1 beta or GM-CSF, and it is downregulated in presence of TGF beta and suramin. Binding of IgA-opsonized targets to CD89 leads to phagocytic and cytotoxic processes of the immunologic defense.SpecificityThe mouse monoclonal antibody ABRA1-01 recognizes human ABRA1 (Abraxas, CCDC98), an adaptor protein involved in DNA repair, which migrates as a 45 kDa band on PAAGE under reducing conditions.Application detailsFlow cytometry: The reagent is designed for analysis of human blood cells using 4 ?l reagent / 100 ?l of whole blood or 10<sup>6</sup> cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
A59
Conjugate:
FITC
Format:
Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
CD89 (Fc-alpha-R) is a type I transmembrane glycoprotein serving as a receptor for IgA. Soluble CD89 is detectable in serum and retains its IgA binding capacity. For signal transduction the association with FcR gamma chain homodimers is needed. CD89 is expressed on granulocytes, monocytes, macrophages, dendritic cells and myeloid cell lines. Its expression is upregulated in presence of IgA immune complexes, stimulators (such as LPS, PMA), TNF alpha, IL1 beta or GM-CSF, and it is downregulated in presence of TGF beta and suramin. Binding of IgA-opsonized targets to CD89 leads to phagocytic and cytotoxic processes of the immunologic defense.SpecificityThe mouse monoclonal antibody A59 recognizes an extracellular epitope of CD89, a 55-100 kDa glycoprotein serving as a receptor for IgA and expressed mainly on granulocytes, monocytes and macrophages.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
A59
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD42b (GPIb alpha) composes together with GPIb beta, GPIX and GPV the GPIb-IX-V receptor complex critical in the process of platelet-rich thrombus formation by tethering the platelet to a thrombogenic surface. CD42b binds to von Willebrand factor (VWF) exposed at a site of vascular injury, as well as to thrombin, coagulation factors XI and XII, high molecular weight kininogen, TSP-1, integrin Mac-1 and P-selectin. The extracellular domain of CD42b by its interactions also contributes to metastasis. Specificity: The mouse monoclonal antibody A59 recognizes an extracellular epitope of CD89, a 55-100 kDa glycoprotein serving as a receptor for IgA and expressed mainly on granulocytes, monocytes and macrophages. Application details: Flow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
AK2
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage:
2-8°C
References 1:
Vettore S et al. Haematologica 2008, 93(11): 1743-7
References 2:
Welsh JD et al. J Thromb Haemost 2012, 10(11):2344-53
p40 is a relatively unknown antibody that recognizes ?Np63-a p63 isoform suggested to be highly specific for squamous/basal cells. In a recent study, p40 is equivalent to p63 in sensitivity for squamous cell carcinoma, but it is markedly superior to p63 in specificity1, which eliminates a potential pitfall of misinterpreting a p63-positive adenocarcinoma or unsuspected lymphoma as squamous cell carcinoma. These findings strongly support the routine use of p40 in place of p63 for the diagnosis of pulmonary squamous cell carcinoma. Postive control Prostate
Cris-3 reacts with CD11a, a transmembrane molecule with 1145 amino acid residues and a MW of 180 kDa. CD11a is expressed on lymphocytes, granulocytes, monocytes and macrophages. Levels on memory T-cells ten to increase. CD11a plays a key role in mediating leukocyte adhesion to endothelium during inflammatory responses through binding to ICAM-1 (CD54). Other ligands are ICAM-2 and ICAM-3. It is also involved in many other T-cell functions and immune phenomena. When paired with CD18, it forms the integrin alphaLbeta2 heterodimer also called LFA-1. LFA-1 is chiefly responsible for lymphocyte adhesion.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
CRIS-3
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Reinherz et. al., EL. Leukocyte Typing II. New York: Springer-Verlag (1985)
Hyb-8 recognizes free biotin and biotinylated proteins (HS biotin, NHS amidocaproylbiotin). The length of the spacer does not affect mAb binding. The antibody also react with biotinylated DNA probes.
AFT14 reacts with Aflatoxin B1 and B2, a 55 kDA protein secreted by Aspergillus. The aflatoxins are a group of closely related mycotoxins that are widely distributed in nature. The most important of the group is aflatoxin B1 (AFB1), which has a range of biological activities, including acute toxicity, teratogenicity, mutagenicity and carcinogenicity. In order for AFB1 to exert its effects, it must be converted to its reactive epoxide by the action of the mixed function mono-oxygenase enzyme systems (cytochrome P450- dependent) in the tissues (in particular, the liver) of the affected animal. This epoxide is highly reactive and can form derivatives with several cellular macromolecules, including DNA, RNA, and protein. Cytochrome p450 enzymes may additionally catalyse the hydroxylation (to AFQ1 and AFM1) and demethylation (to AFP1) of the parent AFB1 molecule, resulting in products less toxic than AFB1. Conjugation of AFB1 to glutathione (mediated by glutathione Stransferase) and its subsequent excretion is regarded as an important detoxification pathway in animals. Aflatoxins are well recognized as a cause of liver cancer, but they have additional important toxic effects. Aflatoxin B1 is a potent hepatocarcinogenic and mutagenic mycotoxin of Aspergillus flavus.
Antibody Isotype:
IgG2a,kappa
Monosan Range:
MONOSAN
Clone:
AFT14/CBL03
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
F S Chu and I Ueno, Appl Environ Microbiol 33(5): 11251128 (1977)
References 2:
Groopman, JD. et al, Proc. Natl. Acad. Sci. USA 81: 7728-7731 (1984)
The neurofilament (NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. RNF403 reacts exclusively with the phosphorylated isoform of the160 kD neurofilament protein.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
RNF403
Concentration:
1 mg/ml
Storage buffer:
PBS with 0.09% sodium azide
Storage:
2-8°C
References 1:
Bauwens et al. Ann Otol Rhinol Laryngol 1992;101:479-486
In lung cancer diagnosis Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is sofar not found in Human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non-SCLC (cell cultures).RNL-4 reacts with peripheral nerves and ganglia of various tissues and cross-reacts with smooth muscle cells and myoepithelium. In the central nervous system it reacts with the neurohypophysis and pars intermedia of the pituitary gland, and a weak diffuse staining was observed in neurons of the granular and molecular layer of the cerebellar cortex, while glial cells, cerebellar medulla and Purkinje cells are negative. Reticulon-1 has been found to indicate neuronal differentiation and to be downregulated in neurological pathologies.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
RNL-4
Concentration:
1 mg/ml
Storage buffer:
PBS with 0.09% sodium azide
Storage:
2-8°C
References 1:
Senden et al. Eur J Cell Biol 1996;69:197-213
References 2:
Senden et al. J Pathol 1997;182:13-21
References 3:
Hens et al. Cell Tissue Res 1998;292:229-237
References 4:
Kim et al. Biochem Biophys Res Comm 2000;276:329-334
In lung cancer diagnosis Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is sofar not found in Human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non-SCLC (cell cultures).RNL-2 recognizes an epitope located within the region of amino acids 421-589 of the neuro-endocrine specific protein Reticulon-1A (NSP-A), which is also present in the N-terminal part of Reticulon-1B (NSP-B). In normal tissues, RNL-2 reacts with brain Purkinje cells, pancreatic islet cells, cells in the pituitary gland and some (peripheral) nerve fibers. In addition, a few epithelia show positive staining.
In lung cancer diagnosis Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is sofar not found in Human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non-SCLC (cell cultures).RNL-2 recognizes an epitope located within the region of amino acids 421-589 of the neuro-endocrine specific protein Reticulon-1A (NSP-A), which is also present in the N-terminal part of Reticulon-1B (NSP-B). In normal tissues, RNL-2 reacts with brain Purkinje cells, pancreatic islet cells, cells in the pituitary gland and some (peripheral) nerve fibers. In addition, a few epithelia show positive staining.
Smoothelin is a constituent of the smooth muscle cell (SMC) cytoskeleton. Antibodies directed to smoothelin are useful tools to monitor SMC differentiation. Smoothelin is exclusively expressed in fully differentiated (contractile) SMCs. RNA and protein analyses revealed a broad species distribution of this protein. Smoothelin has also been detected in smooth-muscle neoplasms. Cells with SMC-like characteristics, such as myofibroblasts and myoepithelial cells, as well as skeletal and cardiac muscle do not contain smoothelin. Confocal scanning laser microscopy of tissue sections and cells in culture show a filamentous organization of smoothelin colocalizing with actin stress fibers. In immunoblots two molecular weight isoforms are detected i.e. a 59 kDa isoform specific for visceral SMC (smoothelin A), and an isoform with a molecular weight of approximately 100 kDa in vascular SMC (smoothelin B). Human smoothelin is encoded by a single copy gene which is loCated on chromosome 22.
The monoclonal antibody 5G5 recognizes human Toll-like receptor 9. Toll-like receptors (TLRs) are highly conserved from Drosophila to humans and share structural and functional similarities. TLRs constitute of a family of pattern recognition receptors (PRRs) that mediate cellular responses to a large variety of pathogens (viruses, bacteria, and parasites) by specific recognition of so-called âpathogen-associated molecular patternsâ. Activation of TLRs, a family of at least 11 differentmembers that function either as homo- or heterodimers, leads to activation of NFκB-dependent and IFNregulatory factor-dependent signaling pathways. TLRs have a central role in innate immunity and are also required for the development of an adaptive immune response. TLRs are expressed by various cells of the immune system, such as macrophages and dendritic cells. They recognize and respond to molecules derived from bacterial, viral and fungal pathogens.<br /> Whereas most TLRs are expressed on the cell surface, TLR9 is expressed intracellularly within one or more endosomal compartments and recognizes nucleic acids. TLR9 detects a rather subtle difference in the DNA of vertebrates compared with that of pathogens. Vertebrate genomic DNAs have mostly methylated CpG dinucleotides where bacterial and viral DNAs have unmethylated CpG dinucleotides. TLR9 undergoes relocation from endoplasmic reticulum to CpG-ODN-containing endosomes. In these endosomes TLR9 becomes a functional receptor after proteolytic cleavage. TLR9 exists as a preformed homodimer and CpG-ODN binding promotes its conformational change, bringing the cytoplasmic TIR-like domains close to each other. This allows a recruitment of the key adapter protein MyD88 which initiates a signalling cascade. The only human immune cell types known to constitutively express TLR9 and to be activated by CpG ODN are pDCs and B cells. TLR9 triggering induces an activation phenotype in the B cells and pDCs, characterized by the expression of costimulatory molecules, resistance to apoptosis, and induces Th1-type immune response profiles.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
5G5
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Ahmad-Nejad; P et al. Eur J Immunol 2002; 32: 1958
Nectin (originally isolated as poliovirus receptor-related protein (PRR)) is a cell-cell adhesion molecule of the immunoglobulin supergene family. Nectin is colocalized with afadin at cadherin-based cell-cell adherence junctions in various tissues and cell lines. The nectin family consist of nectin-1, nectin-2 and nectin-3. Nectin-2 also known as PRR2 or CD112 is a plasma membrane adhesion molecule localized at adherens junctions which is widely expressed in various cell lines including neuronal, endothelial, epithelial and hematopoietic cells. Next to its role in adherens junctions it functions as alphaherpus virus receptor and acts also as intercellular adhesion molecule and pseudorabies virus receptor. Disruption of mouse nectin-2 leads to infertility of male mice. Monoclonal antibody 502-57 has been raised against the extracellular domain of mouse nectin-2. The antibody cross reacts with human nectin-2.
Vimentin (57 kDa) is the most ubiquituos intermediate filament protein and the first to be expressed during cell differentiation. All primitive cell types express vimentin but in most non-mesenchymal cells it is replaced by other intermediate filament proteins during differentiation. Vimentin is expressed in a wide variety of mesenchymal cell types - fibroblasts, endothelial cells etc., and in a number of other cell types derived from mesoderm, e.g., mesothelium and ovarian granulosa cells. In non-vascular smooth muscle cellsand striated muscle, vimentin is often replaced by desmin, however, during regeneration, vimentin is reexpressed. Cells of the lymfo-haemopoietic system (lymphocytes, macrophages etc.) also express vimentin, sometimes in scarce amounts. Vimentin is also found in mesoderm derived epithelia, e.g. kidney (Bowman capsule), endometrium and ovary (surface epithelium), in myoepithelial cells (breast, salivary and sweat glands), an in thyroid gland epithelium. In these cell types, as in mesothelial cells, vimentin is coexpressed with cytokeratin.Furthermore, vimentin is detected in many cells from the neural crest. Particularly melanocytes express abundant vimentin. In glial cells vimentin is coexpressed with Glial Fibrillary Acidic Protein (GFAP). Vimentin is present in many different neoplasms but is particulary expressed in those originated from mesenchymal cells. Sarcomas e.g., fibrosarcoma, malignt fibrous histiocytoma, angiosarcoma, and leio- and rhabdomyosarcoma, as well as lymphomas, malignant melanoma and schwannoma, are virtually always vimentin positive. Mesoderm derived carcinomas like renal cell carcinoma, adrenal cortical carcinoma and adenocarcinomas from endometrium and ovary usually express vimentin. Also thyroid carcinomas are vimentin positive. Any low differentiated carcinoma may express some vimentin. Vimentin is frequently included in the so-called primary panel (together with CD45, cytokeratin, and S-100 protein). Intense staining reaction for vimentin without coexpression of other intermediate filament proteins is strongly suggestive of a mesenchymal tumour or malignant melanoma.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
VI-RE/1
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
STAT1 (signal transducer and activator of transcription 1) is a transcription factor that plays important roles in growth arrest, apoptosis promoting and tumour suppression. After ligation of cytokine receptors STAT1 becomes phosphorylated on Tyr701 by Janus kinase JAK1 or JAK2, dimerizes, translocates to nucleus and contacts DNA. STAT1-STAT2 heterodimers serve as more potent transcriptional inducers than STAT1 homodimers. STAT1 is also phosphorylated on Ser727 by MAPK pathway, independently of tyrosine phosphorylation. However, the both modifications are important for its maximal transcriptional activity. On the other hand, STAT1 phosphorylated on Ser727 is targeted for proteasomal degradation.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
SM1
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD45RB is an of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RB is expressed e.g. in microglia and inflammatory cells.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-143
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Syk is a cytoplasmic protein tyrosine kinase that translocates to the plasma membrane upon B cell antigen receptor (BCR) or the high-affinity IgE receptor (FcepsilonRI) triggering, and phosphorylates downstream adaptor proteins, thereby providing docking sites for initiation of subsequent signaling pathways, such as calcium mobilization, cytoskeleton remodeling, or transcription of specific genes. Syk binds to the receptor assemblies through interactions of its pair of SH2 domains with ITAM motives of the receptor, which have been phosphorylated by Src-family kinases. These kinases also help to activate Syk by phosphorylation of its activation loop.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
SYK-01
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
NTAL (non-T cell activation linker), also known as LAB (linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
NAP-07
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
NTAL (non-T cell activation linker), also known as LAB (linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
NAP-03
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
PAG-C1
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
HLA-E (human leukocyte antigen E) is a non-classical MHC I antigen, which is important for dialogue with NK cells and their regulation through interaction with CD94/NKG2 receptor. Like other MHC I molecules, transmembrane HLA-E molecule (45 kDa) associates with beta2 microglobulin. Unlike HLA-G, expression of HLA-E molecules is not so restricted, but it has been detected at least at mRNA level in virtually all cells and tissues examined. In peripheral blood, HLA-E protein is expressed at least in all mononuclear cells, but in different quantity (B cells and monocytes more than T cells and NK cells).
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-E/06
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.
Antibody Isotype:
IgG3
Monosan Range:
MONOSAN
Clone:
MEM-123
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-147
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
HLA-E (human leukocyte antigen E) is a non-classical MHC I antigen, which is important for dialogue with NK cells and their regulation through interaction with CD94/NKG2 receptor. Like other MHC I molecules, transmembrane HLA-E molecule (45 kDa) associates with beta2 microglobulin. Unlike HLA-G, expression of HLA-E molecules is not so restricted, but it has been detected at least at mRNA level in virtually all cells and tissues examined. In peripheral blood, HLA-E protein is expressed at least in all mononuclear cells, but in different quantity (B cells and monocytes more than T cells and NK cells).
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-E/02
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
The monoclonal antibody 8F4 recognizes mouse myeloperoxidase (MPO). MPO is a glycoprotein produced as a single precursor, which is subsequently cleaved into a alpha and beta chain. In human the biologically active MPO is a 150kDa tetramer composed of 2 glycosylated alfa chains of 59-64 kDa and 2 beta chains of 14 kDa. MPO is stored in azurophilic granules of polymorphonuclear leukocytes and is rapidly released into the phagosome and extracellular space during inflammatory conditions.The enzyme catalyzes the conversion of chloride and hydrogen peroxide to hypochlorite, a potent oxidant, which functions in host defense against microorganisms. </br> Involvement of MPO has been described in several human diseases, such as cardiovascular disease, airway inflammation, lung cancer, Alzheimer's disease and multiple sclerosis. A positive correlation between elevated MPO levels in serum and cardiovascular disease suggest an interesting role for MPO as an diagnostic marker, making it possible to identify patients at risk for future cardiac events. Furthermore, there are some autoimmune diseases, in which MPO is targeted by antineutrophil cytoplasm antibodies. Studies with MPO-knockout mice have shown an increased susceptibility to pneumonia following intratracheal infections. Moreover, MPO deficient mice are more susceptible to experimental autoimmune encephalitis, a T cell-dependent neuronal disease, and have an increased expression of arteriosclerotic plaques compared to wild-type mice.</br> The anti-mouse MPO monoclonal antibody 8F4 recognizes natural MPO in biological solutions by ELISA, in frozen tissue sections fixed with acetone and in flow cytometry using a cell permeabilization method.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
8F4
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Huugen; D et al. Am J Pathol 2005; 167: 47
References 2:
Wang, D et al Blood 2006, 108:1223
References 3:
Matthijsen; M et al. Am J Pathol 2007; 171: 1743
References 4:
Leeuwen van M et al. Arterioscler Thromb Vasc Biol 2008; 28: 84
The monoclonal antibody 15-2 recognizes the mannose receptor (MR), also known as CD206, a member of the vertebrate C-type lectin family. The mannose receptor, is a pattern recognition receptor that is involved in both innate and adaptive immunity. The 175 kDa single-pass type I transmembrane receptor consists of 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail.<br /> The structure is shared by the family of multi lectin mannose receptors: the phospholipase A2-receptor, DEC 205 and the novel C-type lectin receptor (mannose receptor X). The MR binds high-mannose structures on- a wide range of gram positive and gram negative bacteria, yeasts, parasites and mycobacteria. The MR has also been shown to bind and internalize tissue-type plasminogen activator.<br /> MR's are present on monocytes and dendritic cells (DC) and are presumed to play a role in innate and adaptive immunity, the latter via processing by DC. The expression of MR as observed in immunohistology is present on tissue macrophages, dendritic cells, a subpopulation of endothelial cells, Kupffer cells and sperm cells. The expression of MR on monocytes increases during culture and can be enhanced by cytokines as IFN-gamma. Labeling of MR expressing monocytes/macrophages increases with prolonged incubation time probably due to internalization of the MR-antibody-complex. The monoclonal antibody 15-2 prevents binding of glycoproteins including t-PA to MR.<br /> Detection of the MR with anti-MR monoclonal antibody 15-2 can substitute staining for mannose containing probes as labeled mannosylated BSA, a technique which is more cumbersome and less specific.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
15-Feb
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Barret-Berghoeff; M et al. Thromb Haemostas 1997; 77: 718
CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of the immunoglobulin family that serves as an adhesive molecule and is ubiquitously expressed in the nervous system in isoforms ranging from 120-180 kDa. CD56 is found on T cells and NK cells, and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity. Polysialic modification results in reduction of CD56-mediated cell adhesion. Through its extracellular region, CD56 mediates homophilic and heterophilic interactions by binding extracellular matrix components such as laminin and integrins.
Antibody Isotype:
IgG2a, k
Monosan Range:
MONOSAN
Clone:
MEM-188
Conjugate:
Tri-Color
Concentration:
n/a
Storage buffer:
PBS with 4mg/ml BSA, 10% sucrose and 0.1% sodium azide
CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of the immunoglobulin family that serves as an adhesive molecule and is ubiquitously expressed in the nervous system in isoforms ranging from 120-180 kDa. CD56 is found on T cells and NK cells, and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity. Polysialic modification results in reduction of CD56-mediated cell adhesion. Through its extracellular region, CD56 mediates homophilic and heterophilic interactions by binding extracellular matrix components such as laminin and integrins.
Antibody Isotype:
IgG2a, k
Monosan Range:
MONOSAN
Clone:
MEM-188
Conjugate:
R-PE
Concentration:
n/a
Storage buffer:
PBS with 4mg/ml BSA, 10% sucrose and 0.1% sodium azide
CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of immunoglobulin family serving as adhesive molecule which is ubiquitously expressed in nervous system, usually as 120 kDa, 140 kDa or 180 kDa isoform, and it is also found on T cells and NK cells. Polysialic modification results in reduction of CD56-mediated cell adhesion and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity. CD56 is a widely used neuroendocrine marker with a high sensitivity for neuroendocrine tumours and ovarian granulosa cell tumours.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
MEM-188
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-233
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD105 (endoglin) is a homodimeric transmembrane glycoprotein serving in presence of TGFbetaR-2 as a receptor for TGFbeta-1 and TGFbeta-3. CD105 is highly expressed on endothelial cells and promotes angiogenesis during wound healing, infarcts and in a wide range of tumours and its gene expression is stimulated by hypoxia. CD105 prevents apoptosis in hypoxic endothelial cells and also antagonises the inhibitory effects of TGFbeta-1 on vascular endothelial cell growth and migration. Normal cellular levels of CD105 are required for formation of new blood vessels.
CD105 (endoglin) is a homodimeric transmembrane glycoprotein serving in presence of TGFbetaR-2 as a receptor for TGFbeta-1 and TGFbeta-3. CD105 is highly expressed on endothelial cells and promotes angiogenesis during wound healing, infarcts and in a wide range of tumours and its gene expression is stimulated by hypoxia. CD105 prevents apoptosis in hypoxic endothelial cells and also antagonises the inhibitory effects of TGFbeta-1 on vascular endothelial cell growth and migration. Normal cellular levels of CD105 are required for formation of new blood vessels.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
MEM-226
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
The monoclonal antibody BV12 recognizes junctional adhesion molecule-A (JAM-A), also known as JAM-1 and the mouse platelet F11-Receptor (F11R), is a cell adhesion molecule (CAM). JAM-A is a member of the immunoglobulin superfamily found on the surface of mouse platelets and at intercellular junctions of endothelial cells and epithelial cells. JAM-A belongs together with JAM-B (VE-JAM or JAM-3) and JAM-C (JAM-2) to a family of adhesion proteins with a V-C2 immunoglobulin domain organization. JAM plays an important role in tight junctions where it is involved in cell-to-cell adhesion through homophilic interaction. It codistributes with other tight junction components as ZO-1, 7H6 antigen, cingulin and occludin. In human JAM-A plays a role in platelet aggregation, secretion, adhesion and spreading.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
BV12
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Bazzoni; G et al. J Biol Chem 2000; 275: 20520
References 2:
Martinez-Estrada, O et al Am J Physiol Lung Cell Mol Physiol 2005, 288: L1081
The monoclonal antibody 5G5 reacts with the Toll-like receptor 9 (TLR9, CD289). TLRs are highly conserved throughout evolution and have been implicated in the innate defence to many pathogens. In Drosophila, toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defences. In mammals, TLRs identified as type I transmembrane signalling receptors with pattern recognition capabilities, have been implicated in the innate host defence to pathogens. As investigated so far all functional characterized TLR signal via the TLR/IL-1 receptor (IL-1R) pathway where recruitment of MyD88 seems to be essential. In contrast to cell-wall components, bacterial DNA is probably invisible for immune cells until DNA is liberated during processes taking place in the endosomal/lysosomal compartment where intracellular TLR9 recruits MyD88 to initiate signal transduction. Unmethylated CpG-dinucleotide-containing sequences are found much more frequently in bacterial genomes than in vertebrates genomes, whereas the frequency of CpG dinucleotides are suppressed and usually methylated. The regions adjacent to the CpG dinucleotides also affect the immunostimulatory activity. The optimal sequence differs significantly between mammalian species. Methylated CpG dinucleotides lack immunostimulatory activities. Cellular activation in response to bacterial DNA and synthetic dinucleotides containing unmethylated CpG-dinucleotides is mediated by TLR9. The monoclonal antibody 5G5 reacts with RAW macrophages and TLR9 transfected HEK293 cells, and it is cross reactive with canine TLR9.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
5G5
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Ahmad-Nejad; P et al. Eur J Immunol 2002; 32: 1958
CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing, it can thus serve e.g. as a modulator of macrophage recruitment in response to pathogen. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells.
CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing, it can thus serve e.g. as a modulator of macrophage recruitment in response to pathogen. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-263
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
The human intracellular serpin proteinase inhibitor 9 (PI9) is the only human protein able to inhibit the activity of the serine protease granzyme B. Granzyme B is expressed by cytotoxic lymphocytes and induces rapid target cell apoptosis. PI9-17 Mab was selected after immunization with full length recombinant PI9 produced in Escherichia coli. No cross reactivity with other, homologous serpins (PI6, PI8 and PAI-2) was observed.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
PI9-17
Concentration:
250 ug/ml
Storage buffer:
serumfree culture supernatant with 0.7% BSA and 0.1% sodium azide
Storage:
2-8°C
References 1:
Bird CH et al. Mol.Cell.Biol 1998; 18: 6387
References 2:
Bladergroen BA et al. J of Immunol 2001; 166: 3218-325
CD222 (CIMPR, cation-independent mannose 6-phosphate receptor; IGF2 receptor) is a ubiquitously expressed 250 kDa transmembrane protein. No more than 10% of CD222 is present on the cell surface where it serves as a multifunctional receptor. Intracellular (major) fraction of CD222 is involved in transport of newly synthesized lysosomal enzymes modified by mannose 6-phosphate from Golgi apparatus to lysosomes. The cell surface CD222 binds and internalizes exogeneous mannose 6-phosphate-containing ligands. Importantly, CD222 is crutial for internalization and degradation of insulin-like growth factor 2, thus controling cell growth. CD222 also complexes CD87 (urokinase-type plasminogen-activator receptor), plasminogen and latent TGF-beta, last but not least CD222 serves as a receptor for heparanase and even for Listeria.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-238
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD177 (NB1/HNA-2a and PRV-1 form) is a GPI-anchored glycoprotein present mainly on neutrophils. Its plasma membrane expression is increased during pregnancy and and inflammation or after G-CSF application. Ligand of CD177 has been identified as CD31 (PECAM-1). CD177 participates in neutrophil transmigration and seems to be also a pro-proliferative molecule. The antibodies against CD177 can be involved in neonatal alloimmune neutropenia (NAN).
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-166
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Lck is a Src-family tyrosine kinase, which is essential for signaling through the T-cell receptor (TCR) complex. Upon TCR triggering, Lck phosphorylates the ITAM motives in its zeta subunits, establishing binding sites for the SH2 domains of the tyrosine kinase ZAP70, which is also phosphorylated by Lck and thereby activated to generate subsequent signaling platforms by phosphorylation of adaptor LAT. Whereas the majority of Lck is localized to the plasma membrane, there is also a significant fraction associated with the Golgi apparatus, which may contribute to Raf activation under conditions of weak stimulation through the TCR. Lck is also involved in the regulation of apoptosis induced by various stimuli, but not by the death receptors.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
LCK-01
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
BM-5 is specific marker for human myeloid cells and an early marker of myeloid differentiation. It recognizes a nuclear and cytoplasmic antigen present in granulocytes, monocytes, and myeloid precursor cells. It also reacts with a subset of myeloid leukemia cells. BM-5 has no reactivity with any other cell type in human tissues.
BM-4 recognizes a nuclear antigen expressed in human granulocytes (83%) monocytes (20%) and myeloid precursor cells residing in lymphoid and non-lymphoid tissues. BM-4 is an early marker of myeloid differentiation. It also reacts with a subset of myeloid leukemia cells. BM-4 has no reactivity with any other cell type in human tissues.
Induction studies using HL-60 cells showed that BM-3 identifies a nuclear antigen which is expressed during the early phases of myeloid differentiation, making BM-3 an early marker of myeloid differentiation. It is found in 98% of human granulocytes, in 80% of human monocytes and in myeloid precursor cells, residing in lymphoid and non-lymphoid tissues. It also reacts with a subset of myeloid leukemia cells. BM-3 has no reactivity with any other cell type in human tissues. In experiments using S-35 methionine labeled human myeloid leukemia cells BM-3 immunoprecipitated a 13 kDa protein.
CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-241
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Human blood group A antigen belongs to a group of carbohydrate determinants carried on both glycolipids and glycoproteins; it is detected on erythrocytes and certain epithelial cells. The antibody HE-195 recognizes human blood group A including weak variants A3 , AX , A3B, AXB. The specifity of antibody HE-195 was confirmed by comparison of specifity and reactivity to standard reagent using >5.000 samples of blood. Agglutination test A1, A1B, A2B.
Human blood group A antigen belongs to a group of carbohydrate determinants carried on both glycolipids and glycoproteins; it is detected on erythrocytes and certain epithelial cells. The antibody HE-193 recognizes human blood group A (monofucosyl and difucosyl A antigens with chain types 1 and 2, A antigens with chain types 3, 4, 5, 6) and Forssman antigen.
The antibody HEB-29 reacts with human blood group B. Agglutination test + B, A1B, A2B. The specifity of the antibody HEB-29 was confirmed by comparison of specifity and reactivity to standard reagent using >5 000 samples of blood.
The antibody HE-24 distinguishes blood group A1B from A2B. The specifity of the antibody HE-24 was verified on >1000 samples of blood. Agglutination test A1, A1B.
The mouse monoclonal antibody HE-10 agglutinates erythrocytes of group A, and is excellent as a tumour marker in patients of blood group B and 0. It does not agglutinate erythrocytes of group B and 0. Study with specific oligosaccharides showed that the antibody HE-10 reacts with A and H antigens with chain types 3 and 4 and it does not react with A disaccharide, A trisaccharide, A type 1, A type 2, ALeb. The antibody HE-10 does not react with normal tissue sections of donors with blood group B and 0 but it reacts specifically with malignant tissues.
100-1A5 reacts with CD1b, a 44KDa type 1 glycoprotein associated with beta2-microglobulin. It is expressed on dendritic cells, Langerhans cells, thymocytes and T acute lymphoblastic leukemia cells. CD1, type 1 membrane protein, has structural similarity to the MHC class 1 molecule and has been shown to present lipid antigens for recognition by T lymphocytes. CD1b is also expressed in Langerhans interdigitating cells. 100-1A5 also reacts with pyramidal cells in the brain and was typed at the IVth International CD Workshop.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
100-1A5
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Knapp et. al., W (1989). Leucocyte typing IV. Oxford University Press
Until recently, immunological markers for myeloid cells have been lacking, especially those which identify different levels of cellular differentiation. The BM series provides a new panel of monoclonal antibodies which stain early precursor and mature forms of human myeloid cells. This panel of monoclonal antibodies reacts with antigenic determinants present in normal myeloid cells and leukemias of similar derivation. BM-2 recognizes a cytoplasmic antigen expressed in mature human granulocytes (polys) residing in lymphoid and non-lymphoid tissues. It does not react with any other cell type in human tissues.
BM-1 is reactive in B5 fixed, paraffin embedded tissue sections to human myeloid cells and derived malignancies. The antibody reacts with a 183 kDa cytoplasmic antigen with DNAbinding characteristics which is expressed in most myeloid precursor cells and myeloid leukemias. BM-1 is positive on myeloid precursors in bone marrow but lymph nodes are negative. Tissue granulocytes are positive as well as scattered cells in peripheral cortex and interlobular septae of adult and fetal thymus. Portal regions of fetal liver (18 weeks) are also positive
19-OLE reacts with H type 2 antigen, the basis of the ABO blood group system, involving three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity. It is expressed on endothelial cells, epithelial cells and granulocytes. Increased expression of this antigen has been observed on some tumor tissues such as gastric carcinomas, urothelial carcinomas, and colon carcinomas.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
19-OLE
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bara, J. et al. Blood transfusion and immunohaematology, Ph Rouger, D Anstee and Ch Salmon (Eds), Arnette, France 30 (5), p. 685-692, (1987)
References 2:
Marionneau, S. et al. Gasterenterology, 122: 1967-1877 (2002)
References 3:
Garcher, K. et al. Invest Ophthalmol Vis Sci. 35: 1184-1191 (1994)
CD147 is a transmembrane glycoprotein of the immunoglobulin superfamily. It is expressed more intensely on thymocytes than on mature peripheral blood T cells. CD147 is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. CD147 is involved in the regulation of matrix remodeling at the epidermal-dermal interface. It stimulates the production of interstitial collagenase, gelatinase A, stromelysin-1 and various metalloproteinases (MMPs) by fibroblasts. These enzymes, which are typically increased during tissue degradation and wound healing, are important factors in cancer invasion and metastasis.
The monoclonal antibody BV4 recognizes human beta3 integrin subunit present in Platelet glycoprotein GPIIb-IIIa (integrin alphaIIb/beta3, CD41/CD61) and in the vitronectin receptor (integrin alphaV/beta3, CD51/CD61). Intergins are a family of heterodimeric membrane glycoproteins expressed on diverse cell types which function as the major receptors for extracellular matrix and as cell-cell adhesion molecules. As adhesion molecules they play an important role in numerous biological processes such as platelet aggregation, inflammation, immune function, wound healing, tumour metastasis and tissue migration during embryogenesis. In addition integrins are involved in signaling pathways, transmitting signals both into an out from cells. All integrins consist of two non-covalently associated subunits, alpha and beta. At least 12 different alpha subunits and 8 beta subunits have been identified. The beta subunits all contain 56 conserved cysteines (except beta4 which has 48) which are arranged in four repeating units. The beta3 subunit is a 93kDa protein that contains a large loop in the N-terminus stabilized by intrachain disulphide bonding with the first cysteine-rich repeat.<br /> Platelet glycoprotein GPIIb-IIIa is expressed on platelets and megakaryoblasts. It is constitutively expressed and becomes activated on triggered platelets. Platelet glycoprotein GPIIb-IIIa binds to fibrinogen, fibronectin, vWF, vitronectin and thrombospondin. Next to this it is also a receptor for several soluble adhesive proteins.Vitronectin receptor is expressed on endothelial cells, some B cells, monocytes/macrophages, platelets and tumour cells. Vitronectin receptor binds next to vitronectin to fibrinogen, vWF, thrombospondin, fibronectin, osteopontin and collagen. Defects in human beta3 integrin are a cause of Glanzmann thrombasthenia, which is an autosomal recessive disorder characterized by mucocutaneous bleeding and the inability of this integrin to recognize macromolecular or synthetic peptide ligands.
The monoclonal antibody BV3 recognizes human alpha-V/beta-3 integrin present on human cells. Integrins are a superfamily of ?β heterodimeric cell-surface adhesion receptors found in many species. They are expressed on a variety of cells and mediate numerous physiological processes, including inflammation, migration, adhesion and proliferation. The β3 family consist of 2 members: ?IIbβ3 and ?vβ3, which mediate cell-cell and cell-ECM interactions and are important for cellular migration, regulation of gene expression, cell survival, adhesion and differentiation. All processes which are involved in tissue development, angiogenesis and thrombosis. Each subunit consist of an extracellular domain, a single transmembrane segment and a cytoplasmic tail. They connect to the actin cytoskeleton via adaptor proteins that bind theircytoplasmic tails. Cell matrix adhesions also act as signaling units by their capacity to organize the actin cytoskeleton and to accumulate various signaling intermediates. Integrin ?vβ3 was originally identified as the vitronectin receptor. Nevertheless, other ligands include fibrinogen, fibronectin, laminin, thrombospondin, Von Willebrand factor, tenascin, osteopontin and several forms of collagen. The interactions of integrin ?vβ3 to those ligands is mediated by the RGD (Arg-Gly-Asp) sequence motif present in these proteins. Deregulation of β3 integrins is involved in e.g. autoimmune diseases, cardiovascular disorders, transplant rejection and tumorigenesis. In contribution to the latter, integrin ?vβ3 contribute by supporting growth of small (tumor) blood vessels thereby potentiating the metastatic potential. Overexpression of integrin ?vβ3 has been demonstrated in various tumors and activated endothelium.
The monoclonal antibody BV7 recognizes human Ã1-integrin. Beta-1 integrin is a ubiquitously expressed ~89 kDa type I transmembrane protein functioning as receptor when heterodimerized with one alpha subunit. It belongs to the integrin beta chain family consisting of four different genes, encoding multiple β-integrins via alternative splicing. Ligand-recognition depends on the composition of the heterodimer: either collagen, fibronectin, VCAM1, laminin, cytotactin, osteopontin, epiligrin, thrombospondin and CSPG4 can bind to the integrin-complex. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform beta-1B interferes with isoform beta-1A resulting in a dominant negative effect on cell adhesion and migration (in vitro). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. When associated with ?7, β1-integrin regulates cell adhesion and laminin matrix deposition. BV7 is active on HT-29 colon carcinoma cells and on HCCP-2998 tumor cells. It is involved in promoting endothelial cell motility and angiogenesis. Furthermore, β1-integrin plays a mechanistic adhesive role during telophase, and is required for the successful completion of cytokinesis. Upon activation integrins in general, including β1-integrin, are known to exhibit global structural rearrangements and exposure of ligand binding sites. β1-integrin modulation is of importance in tissue repair and regeneration. In cultured primary hippocampal neurons, astrocytes and tissues, cell surface expression of amyloid beta fibrils (key hallmark of Alzheimerâs disease) selectively co-localized with β1-integrin. Preincubation of cells with antibodies against β1-integrin, as well as ?1-integrin, greatly enhanced amyloid beta-induced apoptosis, indicating a protective role for integrins in apoptosis. The monoclonal antibody BV7 does not recognize ?5β1 complex and not the cytoplasmic part of the β1-subunit. Monoclonal antibody BV7 is active on HT-29 colon carcinoma cells and on HCCP-2998 tumor cells. BV7 binds to several other tumor cells (MG3 osteosarcoma, A375 melanoma, MHCC- 1410 and Lovo colon carcinoma) but does not affect adhesion to endothelial cells.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
BV7
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Martin-Padura; I et al. J Biol Chem 1994; 269: 6124
The monoclonal antibody recognizes rat CD8 antigen (MW 34 kDa and 39 kDa) and is reactive with all common inbred strains. 15-11C5 is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from Balb/c mice immunized with WAG/Rij spleen cells.
The monoclonal antibody recognizes rat CD4 antigen (MW 52 kDa) and is reactive with all common inbred strains. 15-8A2 is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from Balb/c mice immunized with WAG/Rij spleen cells.
The monoclonal antibody 67D3 recognizes human heart-type fatty acid-binding protein (H-FABP) of both natural and recombinant origin. The H-FABP protein is derived from the human FABP3 gene. FABPs are small intracellular proteins (~13-14 kDa) with a high degree of tissue specificity that bind long chain fatty acids. They are abundantly present in various cell types and play an important role in the intracellular utilization of fatty acids, transport and metabolism. There are at least nine distinct types of FABP, each showing a specific pattern of tissue expression. Due to its small size, FABP leaks rapidly out of ischemically damaged necrotic cells leading to a rise in serum levels. Ischemically damaged tissues are characterized histologically by absence (or low presence) of FABP facilitating recognition of such areas. H-FABP is localized in the heart, skeletal and smooth muscle, mammary epithelial cells, aorta, distal tubules of the kidney, lung, brain, placenta, and ovary. Furthermore, this antibody is useful for the purification of H-FABP.
The monoclonal antibody 66E2 recognizes human heart fatty acid binding protein (H-FABP) of both natural and recombinant origing. The H-FABP protein is derived from the human FABP3 gene. FABPs are small intracellular proteins (~13-14 kDa) with a high degree of tissue specificity that bind long chain fatty acids. They are abundantly present in various cell types and play an important role in the intracellular utilization of fatty acids, transport and metabolism. There are at least nine distinct types of FABP, each showing a specific pattern of tissue expression. Due to its small size, FABP leaks rapidly out of ischemically damaged necrotic cells leading to a rise in serum levels. Ischemically damaged tissues are characterized histologically by absence (or low presence) of FABP facilitating recognition of such areas. H-FABP is localized in the heart, skeletal and smooth muscle, mammary epithelial cells, aorta, distal tubules of the kidney, lung, brain, placenta, and ovary. The monoclonal antibody 66E2 stains heart muscle cells and striated skeletal muscle cells in immunohistology. It can be used to detect ischemia areas of human heart. It is also useful as marker for brain damage. Furthermore, this antibody is useful for the purification of H-FABP.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
66E2
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Roos; W et al. J Immunol Meth 1995; 183: 149
References 2:
Guillaume, E et al Proteomics 2003, 3: 1495
References 3:
Zimmermann-Ivol; C et al. Mol Cell Proteomics 2004; 3: 66
The antibody reacts with bacteria in suspension and is therefore useful for detection of Proteus spp. in bacterial suspensions. Various systems can be used with this antibody to detect the presence of Proteus spp.. The antibody reacts with approximately 50% of the Proteus spp. isolates tested.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
31-17
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide and 0.1% BSA
Storage:
2-8°C
References 1:
Widjojoatmodjo, M.N., et al., 1991, Eur. J. Clin. Microbio. Infect. Dis. 10, 935-38
133-1C6 reacts with human CD100, a 100 kDa homodimer cell-surface antigen that is expressed on resting and PHA-stimulated T cells. It is absent from bone marrow, erythrocytes, eosinophils and endothelial cells. The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor T cell lines. It plays a role in homotypic cell adhesion and in T cell activation.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
133-1G6
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Knapp, W. et. al. (eds)., Leucocyte Typing IV, Oxford Univ. Press, pp 193-196 (1989)
References 2:
Kishimoto T et al. (eds) Leukocyte Typing VI, Garland Publishing, New York, (1997)
CD98 exists as a heterodimer containing a disulphide-linked glycosylated heavy chain and a nonglycosylated light chain. It is a member of the solute carrier family and encodes a cell surface, transmembrane protein. The protein exists as the heavy chain of a heterodimer, covalently bound through disulphide bonds to one of several possible light chains. The encoded transporter plays a role in regulation of intercellular calcium levels and transport L-type amino acids. Alternatively spliced transcript variants, encoding different isoforms, have been characterized. Monocytes express high levels of CD98 antigen. Peripheral blood T- and B-cells, as well as NKcells and granulocytes express low levels of CD98. Activation of T-cells and NK-cells leads to upregulation of CD98. RBCs are negative. IPO-T10 was typed at the VIth International Workshop and Conference on Human Leukocyte Differentiation Antigens
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
IPO-T10
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
The VIth International Workshop and Conference on Human Leukocyte Differentiation Antigens, Kobe, Japan (1996)
References 2:
Woodhead VE et al. Int Immunol.12(7):1051-61 (2000)
The antibody recognizes lipid A. The lipid A domain of lipopolysaccharide (LPS) is a unique, glucosamine-based phospholipid that makes up the outer monolayer of the outer membrane of most gram-negative bacteria. Lipid A is responsible for the endotoxic activities of LPS, and a heteropolysaccharide. It doens't react very well with RE-LPS (modified Lipid A).
B-R18 specifically recognizes CD95. CD95 is a cell surface glycoprotein with a MW of 40-45 kDa and contains 8 kDa of N-glyosidic-linked polysaccharide. It is a receptor for TNFSF6/FASLG, a member of the nerve growth factor receptor/tumor necrosis factor superfamily, mediating receptor-triggered apoptosis. CD95 is preferentially expressed by the CD54RAlow and CD45ROhigh subset of memory T-cells, but it also found on peripheral monocytes. It is further found on human B-cell lines like pre-B cells, EBV infected cells, Burkitt cells and plasmacytoma cells. It also binds to human T-cell lines, myeloid cell lines, hepatocyte carcinoma and endothelial cells.
The antibody reacts with several Staphylococcus strains using ELISA. It does not react with several members of the Enterobacteriaceae and not with S. Aureus. It is not tested for IHC-P. The epitope is not determined, but it might be a component of the cell wall. The antibody recognizes whole bacteria.
The monoclonal antibody 31 recognizes secretory leukocyte proteinase inhibitor (SLPI). SLPI was identified as an alarm reactant and expression is induced by inflammatory factors like LPS, IL1β, TNF? and neutrophils elastase. SLPI is a relatively small basic antiprotease of 11.7 kDa and is a cationic non-glycosylated protein consisting of 107amino acids. SLPI has a high affinity for the neutrophil serine proteinases, elastase and cathepsin G. Orthologues of SLPI have been found in mice, rate, pigs and sheep. It consists of two highly similar WAP (âwhey acid proteinâ)/four-disulphide core domains. SLPI contain 16 cysteine residues which assemble into eight disulphide bridges (four in each WAP domain). SLPI is constitutively expressed at many mucosal surfaces and is produced by a variety of epithelial cells, including respiratory, intestinal and amniotic epithelia. Expression is also detected in mast cells, neutrophils and macrophages. Expression of SLPI gene is significantly increased by progesterone and by the pro-inflammatory cytokines TNF-? and IL1-β. Although SLPI has been shown to inhibit a spectrum of proteases (including HNE, cathepsin G, trypsin, chymotrypsin and chymase), its main action in this regard is likely to be the inhibition of elastase, as indicated by its low dissociation constant and favourable kinetics of inhibition for this enzyme. SLPI has been described in several body fluids like seminal fluid, bronchial fluids, cervical fluids and saliva. It has been found to be antibacterial, antifungal, anti-retroviral, and to have an important role in mucosal defence. SLPI might also facilitate tumor spread, contributing to wound healing, is elevated in sepsis and levels seem to correlate with oral candidiasis in HIV-1 positive patients. The reactivity of the antibody 31 with isolated domains of SLPI was evaluated using domains obtained by cleavage using partial acidic hydrolysis. Therefore, monoclonal antibody 31 recognizes also other SLPI cleavage products.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
31
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Wingens; M et al. J Invest Dermatol 1998; 111: 996
References 2:
Nakao, R et al Immunology 2003, 109: 271
References 3:
Aarbiou; J et al. Inflamm Res 2004; 53:230-238
References 4:
Tjabringa S et al. FEMS immunol and med microbial 2005; 45:151
CD90 (Thy-1) is an 18-35 kDa GPI-anchored glycoprotein and a member of the immunoglobulin superfamily. It may contribute to inhibition of proliferation/differentiation of hematopoietic stem cells and neuron memory formation in the CNS. It consists of a single Ig domain (112 amino acids; 25-35 kDa) inserted into the cell membrane via a GPI-anchor. Expressed by hematopoietic stem cells and neurons in all species studied. Its highly expressed in connective tissue and various fibroblasts and stromal cell lines, expressed only on small % fetal thymocytes, 10-40% of CD34+ cells in bone marrow, and
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
AF-9
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Cervelló I et al. PLoS One 6: e21221 (2011)
References 2:
Lehmann GM et al. Am J Physiol Cell Physiol 299: C672-81 (2010)
The antibody reacts reacts with a cell wall component of C. jejuni and C. coli. The Hippu¬rate reaction was considered as a marker for C. jejuni. The antibody does not react with C. fetus or C. laridus.
CD46 (MCP, membrane cofactor protein) is a multifunctional cell surface transmembrane protein that binds and inactivates C3b and C4b complement fragments, regulates T cell-induced inflammatory responses by either inhibiting (CD46-1 isoform) or increasing (CD46-2 isoform) the contact hypersensitivity reaction. CD46 also serves as a receptor for several human pathogens (both bacteria and viruses), and its ligation alteres T lymphocyte polarization toward antigen-presenting cells or target cells, inhibiting lymphocyte function. CD46 is a protector of placental tissue and is also expressed on the inner acrosomal membrane of spermatozoa.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-258
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
This antibody reacts with the C-terminus of Nucleophosmin (B23). NPM1 (Nucleophosmin 1, B23, nutramin, NO38) is a ubiquitously expressed phosphoprotein involved in ribosome assembly/transport, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity, centrosome duplication, and regulation of p53. NPM1 continuously shuttles between the nucleus, cytoplasm, nucleolus and chaperoning core histones from the nucleus to the cytoplasm.
Mouse anti Human CD33 antibody, clone WM53 recognizes the human CD33 cell surface glycoprotein. This antigen, considered to be specific for the myeloid lineage, has also been reported to be present on cells of lymphoid origin. Mouse anti Human CD33 antibody, clone WM53 immunoprecipitates a protein of ~75 kDa from myeloid cells, a smaller protein of approximately 67 kDa has been observed in immunoprecipitates from lymphoid targets.
Mouse anti Human CD33 antibody, clone WM53 recognizes the human CD33 cell surface glycoprotein. This antigen, considered to be specific for the myeloid lineage, has also been reported to be present on cells of lymphoid origin. Mouse anti Human CD33 antibody, clone WM53 immunoprecipitates a protein of ~75 kDa from myeloid cells, a smaller protein of approximately 67 kDa has been observed in immunoprecipitates from lymphoid targets.
Antibody reacts with epitope at aa 16-35. DK4 is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a).
HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.
HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
W6/32
Conjugate:
FITC
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
W6/32
Conjugate:
Biotin
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
W6/32
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD19 is a transmembrane glycoprotein of Ig superfamily expressed by B cells from the time of heavy chain rearrangement until plasma cell differentiation. It forms a tetrameric complex with CD21 (complement receptor type 2), CD81 (TAPA-1) and Leu13. Together with BCR (B cell antigen receptor), this complex signals to decrease B cell treshold for activation by the antigen. Besides being signal-amplifying coreceptor for BCR, CD19 can also signal independently of BCR coligation and it turns out to be a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.
CD19 is a transmembrane glycoprotein of Ig superfamily expressed by B cells from the time of heavy chain rearrangement until plasma cell differentiation. It forms a tetrameric complex with CD21 (complement receptor type 2), CD81 (TAPA-1) and Leu13. Together with BCR (B cell antigen receptor), this complex signals to decrease B cell treshold for activation by the antigen. Besides being signal-amplifying coreceptor for BCR, CD19 can also signal independently of BCR coligation and it turns out to be a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
LT19
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD19 is a transmembrane glycoprotein of Ig superfamily expressed by B cells from the time of heavy chain rearrangement until plasma cell differentiation. It forms a tetrameric complex with CD21 (complement receptor type 2), CD81 (TAPA-1) and Leu13. Together with BCR (B cell antigen receptor), this complex signals to decrease B cell treshold for activation by the antigen. Besides being signal-amplifying coreceptor for BCR, CD19 can also signal independently of BCR coligation and it turns out to be a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.
CD19 is a transmembrane glycoprotein of Ig superfamily expressed by B cells from the time of heavy chain rearrangement until plasma cell differentiation. It forms a tetrameric complex with CD21 (complement receptor type 2), CD81 (TAPA-1) and Leu13. Together with BCR (B cell antigen receptor), this complex signals to decrease B cell treshold for activation by the antigen. Besides being signal-amplifying coreceptor for BCR, CD19 can also signal independently of BCR coligation and it turns out to be a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
LT19
Conjugate:
Biotin
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
This monoclonal antibody binds to human PECAM-1 (platelet endothelial cell adhesion molecule-1; CD31) a specific component of endothelial cell junctions. PECAM-1 is also expressed in platelets and leukocytes.
CD97 is a G-protein-coupled seven-span transmembrane adhesive receptor that is constitutively expressed on granulocytes and monocytes and rapidly upregulated on T and B cells upon activation. CD97 is produced in alternatively spliced forms and its cellular ligand is CD55 (DAF), which protects various cell types from complement-mediated damage. Interaction of CD97 on leukocytes and CD55 on vessel cells probably facilitate leukocyte activation and migration into the tissues, similarly, CD97 seems to play a role in tumour migration and invasiveness. CD97 is involved in T cell regulation and peripheral granulocyte homeostasis.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-180
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
MET is a tyrosine kinase receptor for the hepatocyte growth factor. It is linked to functions such as cell proliferation, scattering, morphogenesis, and survival. Ligand binding at the cell surface induces autophosphorylation of MET that provides docking sites for downstream signaling molecules. After activation of the ligand, MET interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, and STAT3.
CD64 (FCGR1A) encodes a protein that plays an important role in the immune response. CD64 is a high-affinity Fc-gamma receptor and is one of three related gene family members located on chromosome 1. CD64 plays an important role in the immune response and its dysfunction has been studied in cervical adenitis and tetrasomy 21.
CD38 (NAD+ glycohydrolase) is a type II transmembrane glycoprotein able to induce activation, proliferation and differentiation of mature lymphocytes and mediate apoptosis of myeloid and lymphoid progenitor cells. CD38 functions as a multi-catalytic ectoenzyme serving as ADP-ribosyl cyclase, cyclic ADP-ribose hydrolase and possibly NAD+ glycohydrolase or as a cell surface receptor. Antibodies to CD38 are useful in subtyping of lymphomas and leukemias, detection of plasma cells (i.e. identification of myelomas), and as a marker for activated B and T cells.
CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor.
CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor.
CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor.
CD20 is a non-glycosylated surface phosphoprotein that has a molecular weight range of 33-37 kDa depending on the degree of phosphorylation. CD20 is expressed on mature and most malignant B cells, in a subpopulation of T lymphocytes and follicular dendritic cells. CD20 expression on B cells is synchronous with the expression of surface IgM and it regulates transmembrane calcium conductance, cell cycle progression and B-cell proliferation.
Antibody Isotype:
IgG3
Monosan Range:
MONOSAN
Clone:
HI47
Conjugate:
R-PE
Concentration:
n/a
Storage buffer:
PBS with 4mg/ml BSA, sucrose and 0.1% sodium azide
CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of kappa or lambda light chains. CD19 appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen.
CD16 (FCGR3A) is a 50-65 kDa cell surface molecule that exists in two forms - a transmembranous form expressed by NK cells and some T cells, and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC by NK cells. Human CD16 is expressed in two forms FCGR3A and FCGR3B. FCGR3A is associated with the FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity.
CD16 (FCGR3A) is a 50-65 kDa cell surface molecule that exists in two forms - a transmembranous form expressed by NK cells and some T cells, and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC by NK cells. Human CD16 is expressed in two forms FCGR3A and FCGR3B. FCGR3A is associated with the FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity.
The antibody reacts with a protein determinant of the extra cellular domain of the human EGFR, and does not cross react with EGFR from murine cells. The antibody shows binding competition with EGF. The antibody reacts in immunoprecipitation with the functional EFR protein-tyrosine kinase complex.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
2E9
Concentration:
100 ug/ml
Storage buffer:
PBS with 1% BSA & 0.1% sodium azide
Storage:
2-8°C
References 1:
Defize L.H.K, et al. The Journal of Cellbiology. 1989: 109:2495-2507
References 2:
Van der Burg M.E.L, et al. Eur J Cancer. 1993: Vol29A, No 14: 1951-1957
Mouse anti Human CD79a antibody, clone HM57 recognizes an epitope within the cytoplasmic domain of CD79a. CD79a, also known as mb-1, is a 45 kDa protein that is expressed by B lymphocytes during differentiation from early pre-B cell stage through to plasma cells. The CD79a molecule associates with CD79b (B29) to form a heterodimer that is non-covalently linked to surface immunoglobulin, forming the B-cell receptor (BCR) complex. The CD79a/CD79b heterodimers are also necessary for intracellular signaling following antigen-binding to surface immunoglobulin.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
HM57
Concentration:
IgG 1 mg/ml
Format:
Purified
Storage buffer:
PBS with <0.1% sodium azide
Storage:
aliquots -20°C. Thawed 2-8°C
References 1:
Mason DY et al. J Immunol 1991; 147: 2474-82
References 2:
Spaas JH Vet J 2013; 195: 107-13
References 3:
De Schauwer C et al. Stem Cell Res Ther 2014; 5: 6
References 4:
Hillmann A et al. Cell transplant 2016; 25: 109-24
Mouse anti Human CD72 antibody, clone Bu40 recognizes human CD72, a ~86 kDa cell surface homodimer expressed on B lymphocytes. CD72 is expressed on early B cell precursors, through to mature activated B cells, but is lost upon terminal differentiation to plasma cells.
Mouse anti Human CD7 antibody, clone LT7 recognizes the human T-cell antigen CD7, also known as TP41 or CD7. CD7 is a single chain cell surface glycoprotein of ~40 kDa, expressed by T cells, NK cells and thymocytes. CD7 is also expressed by tumor cells in some cases of acute myeloid leukaemia.
Mouse anti human CD68 antibody, clone KP1 recognizes the human CD68 cell surface antigen, a ~110 kDa glycoprotein primarily expressed by macrophages and monocytes.
Mouse anti Human CD62P antibody, clone AK-6 recognizes the CD62P, also known as P-selectin, Granule membrane protein 140, GMP140, Leukocyte-endothelial cell adhesion molecule 3 or Platelet activation dependent granule-external membrane protein. CD62P is a 830 amino acid, including a 41 amino acid signal peptide, ~140 kDa single pass type I transmembrane glycoprotein expressed on activated platelets and endothelial cell
Mouse anti Human CD61 antibody, clone Y2/51 recognizes CD61, a ~105 kDa glycoprotein, also known as integrin beta 3 chain. This molecule associates with either the alpha IIb integrin (CD41) or the alpha V integrin (CD51) at the cell surface. CD61 is expressed on platelets and megakaryocytes in association with CD41, and on endothelial cells, monocytes, platelets and osteoclasts in association with CD51.
Mouse anti Human CD49d monoclonal antibody, clone HP2/1 recognizes human CD49d also known as integrin alpha-4 or VLA-4 subunit alpha. CD49d is a ~150kDa single pass type 1 transmembrane glycoprotein with seven FG-GAP repeats, characteristic of alpha integrins, in its extracellular domain. CD49d can be proteolytically cleaved to yield framents of 80 and 70kDa (Hemler et al. 1987). CD49d associates with either CD29 to form VLA-4 or with Integrin beta-7 to form the Peyer's patch-specific homing receptor LPAM-1, involved in the lymphocyte migration and homing to gut-associated lymphoid tissue (Sackstein 2006) through its interaction with MadCam-1, preferentially expressed on Peyers patch high endothelial venules and postcapillary venules in lamina propria (Briskin et al. 1997).
Mouse anti Human CD49b antibody, clone 16B4 recognizes human CD49b, also known as integrin ?2, collagen receptor, Platelet membrane glycoprotein Ia or VLA-2 subunit alpha. CD49b is a 1181 amino acid ~160 kDa single pass type-1 transmembrane glycoprotein possessing multiple FG-GAP repeats and a single VWFA domain. Mouse anti human CD49b antibody, clone 16B4 is identified as capable of immunoprecipitating a non-reducible alpha subunit from I125 surface labeled human cell line extract. It is confirmed as specific to the alpha 2 subunit by relative expression of antigen on various cell lines by FACS, and its recognition of affinity purified alpha 2 beta 1 in dot blots
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
16B4
Concentration:
IgG 1 mg/ml
Format:
Purified
Storage buffer:
PBS with <0.1% sodium azide
Storage:
aliquots -20°C. Thawed 2-8°C
References 1:
Fitter S et al. Biochem J 1999; 338: 61-70
References 2:
Sinock PM et al. J Cell Sci 1999; 112: 833-44
References 3:
Eaton CL et al. Prostate 2010; 70: 875-82
References 4:
Rintanen N et al. Mol Biol Cell 2012; 23: 448-463
References 5:
Poulter NS et al. J Thromb Haemost 2017; 15: 549-564
Mouse anti Human CD47 antibody, clone 1/1A4 recognizes human CD47, also known as integrin-associated protein, a 50kDa glycoprotein expressed on all haematopoietic cell lines, epithelial cells, endothelial cells, fibroblasts and many tumour cell lines. CD47 is involved in several cellular processes including regulation of T cell and dendritic cell activation, regulation of neutrophil (PMN) chemotaxis, caspase-independent apoptosis, and may act as a signal transducer in the regulation of cation fluxes across cell membranes. CD47 has been identified as a ligand for the inhibitory macrophage receptor known as signal regulatory protein alpha (SIRPalpha) and as a receptor for thrombospondin-1.
Mouse anti Human CD45RA antibody, clone F8-11-13 recognizes the human CD45RA cell surface antigen. CD45 is a complex molecule existing in a number of isoforms. Antibodies recognizing a common epitope on all of these isoforms are termed CD45 whilst those recognizing only individual isoforms are termed CD45RA or CD45RO etc. Mouse anti Human CD45RA antibody, clone F8-11-13 reacts with the high molecular weight form of the leucocyte common antigen (LCA) expressed by B lymphocytes and a subset of T lymphocytes. Mouse anti Human CD45RA antibody, clone F8-11-13 is routinely tested in flow cytometry on human peripheral blood lymphocytes
Beta2-microglobulin non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
B2M-01
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Mouse anti Human CD45 antibody, clone F10-89-4 recognizes the human CD45 cell surface antigen, also known as leucocyte common antigen (LCA). CD45 is a complex molecule existing in a number of isoforms. Antibodies recognizing a common epitope on all of these isoforms are termed CD45 whilst those recognizing only individual isoforms are termed CD45RA or CD45RO etc. Mouse anti Human CD45 antibody, clone F10-89-4 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes, having a higher level of expression on lymphocytes than on granulocytes.Mouse anti Human CD45 antibody, clone F10-89-4 is routinely tested in flow cytometry on human peripheral blood leucocytes.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
F10-89-4
Concentration:
IgG 1 mg/ml
Format:
Purified
Storage buffer:
PBS with <0.1% sodium azide
Storage:
aliquots -20°C. Thawed 2-8°C
References 1:
Dalchau R et al. Eur J Immunol 1980; 10: 745-9
References 2:
Hauzer PV et al. Am J Pathol 2010; 177: 2011-21
References 3:
Gogoi P et al. PLoS One 2016; 11: e0147400
References 4:
Ndacayisaba LJ et al. Curr Oncol 2022; 29: 2954-72
Mouse anti Human CD44 antibody, clone F10-44-2 recognizes human CD44, also known as Epican, HCAM, Phagocytic Glycoprotein 1 (PGP-1) or Hyaluronate receptor . Human CD44 is a single pass, type I transmembrane glycoprotein of variable molecular weight ranging from ~90kDa to ~220kDa depending on alternate splicing of the variable region exons and on the degree of glycosylation. CD44 is expressed on multiple cell types and is involved in multiple functions including cell-cell interactions and cell-extracellular matrix binding. Hyaluronan, a high molecular weight polysaccharide component of the extracellular matrix acts as the principal ligand for the CD44 receptor (Laurent and Fraser 1992).
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
F10-44-2
Concentration:
IgG 1 mg/ml
Format:
Purified
Storage buffer:
PBS with <0.1% sodium azide
Storage:
aliquots -20°C. Thawed 2-8°C
References 1:
Dalchau R et al. Eur J Immunol 1980; 10: 745-9
References 2:
Xu L et al. Stem Cell Res Ther 2017; 8: 84
References 3:
Hou B et al. J Biomed Mat Res A 2018; 106: 3065-78
References 4:
Squillace N et al. J Antimicrob Chemother 2018; 73: 2162-70
Mouse anti Human CD43 antibody, clone DFT-1 recognizes human CD43 also known as leukosialin or sialophorin. CD43 is a 381 amino acid single pass type I transmembrane glycoprotein present on the cell surface of multiple cell types including thymocytes, T-lymphocytes, neutrophils, plasma cells and platelets. It is lacking on the surface of most B-lymphocytes. CD34 is a major membrane sialoglycoprotein expressed on T lymphocytes as two predominant glycoforms, a ~115 kDa form present on all T-cells and a ~130 kDa form indicative of T-cell activation (Barren et al. 1997) and acts as a T-cell counter-receptor for CD169/Siglec-1 (van den Berg et al. 2001). Mouse anti Human CD43 antibody, clone DFT-1 binds to both glycoforms of human CD43 (Kyoizumi et al. 2004)
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
DFT-1
Concentration:
IgG 1 mg/ml
Format:
Purified
Storage buffer:
PBS with <0.1% sodium azide
Storage:
aliquots -20°C. Thawed 2-8°C
References 1:
Stross WP et al. J Clin Pathol 1989; 42: 953-61
References 2:
Kojima N et al. Exp Cell Res 1994; 214: 537-42
References 3:
Anzai N et al. Blood 1999; 93: 3317-26
References 4:
Matsuo A et al. J Neuroimmunol 1996; 71: 81-6
References 5:
Woroniecka R et al. Am J Clin Pathol 2015; 143: 25-35
Mouse anti Human CD35 antibody, clone E11 recognizes complement receptor type 1, also known as CD35 or the C3b/C4b receptor. CD35 a single chain, type I single pass transmembrane glycoprotein which exists in four allotypic forms (A,B,C,D) of ~190 kDa, ~220 kDa, ~160 kDa and ~250 kDa respectively (Khera et al. 2009).CD35 is expressed by granulocytes, monocytes, B cells and some T cells. Functionally, CD35 acts as a receptor for the C3b component of complement.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
E11
Concentration:
IgG 1 mg/ml
Format:
Purified
Storage buffer:
PBS with <0.1% sodium azide
Storage:
aliquots -20°C. Thawed 2-8°C
References 1:
Hogg N et al. Eur J Immunol 1984; 14: 236-43
References 2:
Birmingham DJ et al. J Immunol 1996; 157: 2586-92
References 3:
Sopper S et al. Cytometry 1997; 29: 351-62
References 4:
Weissgerber P et al. FEMS Microbiol Lett 2003; 219: 173-9
Mouse anti Human CD314 antibody, clone 1D11 recognizes CD314, also known as natural killer receptor G2 (NKG2D) and as killer cell lectin-like receptor subfamily K, member 1 (KLRK1). CD314 is a C-type lectin-like activating receptor which is expressed on most natural killer (NK) cells, CD8 T cells and gamma delta T cells. CD314 forms homodimers that signal through an associated DAP10 adaptor protein
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
1D11
Concentration:
IgG 1 mg/ml
Format:
Purified
Storage buffer:
PBS with <0.1% sodium azide
Storage:
aliquots -20°C. Thawed 2-8°C
References 1:
Bauer S et al. Science 1999; 285: 727-9
References 2:
Das et al. J Immunol 2004; 172: 6578-86
References 3:
Groh et al. Nat Immunol 2001; 2: 255-60
References 4:
Jinushi M et al. J Immunol 2003; 171: 5423-9
References 5:
Holmen C et al. J Am Soc Nephrol 2007; 18: 2497-508
Mouse anti Human CD29 monoclonal antibody, clone 4B7R recognizes the human integrin beta 1 subunit, also known as CD29, a ~130 kDa (red) 115 kDa (non-red) single pass type I transmembrane glycoprotein expressed by most leucocytes and mesenchymal stem cells.Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. Surface expression of CD29 on human natural killer cells can be reduced by pretreatment with the glutathioneS-transferase inhibitor diethyl maleate (Horvath-Arcidiacono et al. 2003)
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
4B7R
Concentration:
IgG 1 mg/ml
Format:
Purified
Storage buffer:
PBS with <0.1% sodium azide
Storage:
aliquots -20°C. Thawed 2-8°C
References 1:
Marshall HF et al. Br J Cancer 1998; 77: 522-9
References 2:
Pillay J et al. J Leukoc Biol 2010; 88: 211-20
References 3:
Horvath-Arcidiacono JA et al. Cell Immunol 2011; 222: 35-44
References 4:
Kim BS et al. Biosci Biotechnol Biochem 2011; 75: 13-9
Mouse anti Human CD243, clone UIC2 recognizes an extracellular conformational epitope of CD243, also known as MDR1 (multi-drug resistance protein 1) and Pgp (P-glycoprotein), a multi pass transmembrane protein and member of the ABC transporter (ATP-binding cassette) family, containing two ABC transporter type 1 domains and two ABC transporter domains. CD243 acts as an active efflux pump for a diverse range of lipophillic compounds. CD243 is expressed at low levels in the cell membrane of peripheral blood leucocytes, and constitutively expressed on the apical plasma membrane of excretory epithelial cells of the kidney, liver, brain and small intestine. CD243 mediates resistance to many chemotherapeutic agents used for tumour suppression and is therefore of special interest to oncologists. Clone UIC2 is a strong inhibitor of CD243-mediated efflux and of the resistance of MDR cells to CD243 transported cytotoxic drugs.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
UIC2
Concentration:
IgG 1 mg/ml
Format:
Purified
Storage buffer:
PBS with <0.1% sodium azide
Storage:
aliquots -20°C. Thawed 2-8°C
References 1:
Mechetner EB et al. PNAS 1992; 89: 5824-8
References 2:
Park SW et al. Cytometry part A 2003; 53A: 67-78
References 3:
Koziolek MJ et al. Kidney Int 2001; 60: 156-66
References 4:
Meister S et al. Neoplasia 2010; 12: 550-61
References 5:
Tempestili M et al. J Antimicrob Chemother 2014; 69: 1760-6
Mouse anti Human CD21, clone Bu33 recognizes human CD21, a ~145 kDa cell surface glycoprotein expressed by mature B lymphocytes, follicular dendritic cells, subsets of normal thymocytes and subsets of T cells. The CD21 molecule acts as a receptor for CD23, for complement components C3d, C3dg and iC3b and for Epstein Barr Virus. CD21 is part of the signalling complex that is linked to CD19, CD81 and phosphatidylinositol3-kinase.
Mouse anti Human CD161 antibody, clone B199.2 recognizes the human Killer cell lectin-like receptor subfamily B member 1, also known as CD161, C-type lectin domain family 5 member B, HNKR-P1a, NKR-P1A or Natural killer cell surface protein P1A. CD161 is a 225 amino acid ~25 kDa predicted molecular mass, single pass type II transmembrane glycoprotein with a single C-type lectin domain. CD161 is expressed by almost all NK cells and a subset of CD3+ve T cells (Lanier 1994). Mouse anti Human CD161 antibody, clone B199.2 cross-competes with and recognizes a similar epitope to the DX1 monoclonal antibody (Lanier et al. 1994).
Mouse anti Human CD16 antibody, clone DJ130c recognizes human CD16, also known as Low affinity immunoglobulin gamma Fc region receptor III-A or Fc-gamma RIIIa. CD16a is a 254 amino acid ~50-65 kDa single pass type 1 transmembrane glycoprotein bearing two Ig-like C2 type domains. CD16 exists as a transmembranous form (Fc gammaRIIIA, or CD16A) and a glycosyl phosphatidylinositol (GPI) anchored form, Fc gammaRIIIB, or CD16B (Scallon et al. 1989). CD16A is expressed by NK cells, some T cells, and macrophages, whereas CD16B is primarily expressed by granulocytes (Ravetch and Perussia 1989). In addition, CD16B exists as two allelic variants NA1 and NA2 .DJ130c recognizes all polymorphonuclear cells irrespective of their NA phenotype. Mouse anti Human CD16 antibody, clone DJ130c recognizes an epitope in the first membrane-distal domain of CD16, recognizes both CD16a and CD16b and has been demonstrated to cross-react with CD16 from rhesus macaques, Macaca mulatta (Xu et al. 2012)
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
DJ130c
Concentration:
IgG 1 mg/ml
Format:
Purified
Storage buffer:
PBS with <0.1% sodium azide
Storage:
aliquots -20°C. Thawed 2-8°C
References 1:
Schmidt R et al. Diab Vasc Dis Res 2011; 8: 221-8
References 2:
Shantsila E et al. Thromb Haemost 2012; 108: 32-40
Mouse anti Human CD151 antibody, clone 11G5a recognizes the human CD151 cell surface antigen, also known as PETA-3. CD151 is expressed by epithelial cells, endothelial cells, platelets, megakaryocytes, monocytes and in the renal glomeruli and proximal and distal tubules. CD151 is not expressed by lymphocytes or granulocytes. More recently CD151 has also been shown to be expressed by erythrocytes, and to carry the MER2 blood group antigen (Crew et al. 2004).
Mouse anti Human CD11a antibody, clone 38 recognizes the integrin alpha L subunit, also known as CD11a, leukocyte function-associated molecule 1 alpha chain LFA-1 or integrin alpha-L. CD11a is a ~180 kDa glycoprotein expressed by all human leucocytes. CD11a is involved in cell-cell adhesion processes and binds to ICAM-1, 2 and 3. Mouse anti Human CD11a antibody, clone 38 inhibits CD11a mediated adhesion of T cells to ICAM-1 (Stewart et al. 1996).
Mouse anti Human CD102 antibody, clone CBRIC2/2 recognizes human CD102, also known as intercellular adhesion molecule-2 (ICAM-2). CD102 is a glycosylated cell surface protein that is broadly expressed on most leucocytes and is strongly expressed by endothelial cells. CD102 interacts with the CD11a/CD18 (LFA-1). CD102 may play a role in lymphocyte re-circulation and T-cell activation
Mouse anti Human CD10 antibody, clone SN5c recognizes human neprilysin, also known as CD10, atriopeptidase, enkephalinase, neutral endopeptidase 24.11, skin fibroblast elastase or common acute lymphocytic leukemia antigen (CALLA). CD10 is a 749 aminoacid, ~100 kDa single pass type II transmembrane glycoprotein expressed by acute lymphoblastic leukaemia cells and by peripheral blood granulocytes.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
SN5c
Concentration:
IgG 1 mg/ml
Format:
Purified
Storage buffer:
PBS with <0.1% sodium azide
Storage:
aliquots -20°C. Thawed 2-8°C
References 1:
Biddle WC et al. Leuk Res 1989; 13: 699-707
References 2:
Riemann D et al. Biochem J 2001; 354: 47-55
References 3:
Diaz-Romero J et al. J Cell Physiol 2008; 214: 75-83
Mouse anti Human CA125 antibody, clone X325 recognizes the ovarian cancer mucin CA125 (also known as MUCIN 16). CA125 consists of a short cytoplasmic tail, a transmembrane domain and an exceptionally large glycosylated extracellular domain. The extracellular domain is dominated by a large number of 156-amino acid repeat units, over 60 in all. These repeats are known to bind the antibodies OC125 and M-11. CA125 is present within normal ovarian, endometrium, endocervix and fallopian tissue, but levels are elevated in over 90% of women with advanced ovarian cancer. CA125 levels may also be elevated in other cancers and non-cancerous conditions such as peritonitis, endometriosis and liver cirrhosis. Levels may also fluctuate during the menstrual cycle and occasionally increases in pregnancy, making CA125 an unreliable marker for ovarian cancer in premenopausal women. The function of CA125 in healthy tissue is unknown but it is thought to provide a protective lubricating barrier against particles and infectious agents at mucosal surfaces
Mouse anti Human beta endorphin antibody, clone B31.15 reacts with beta endorphin, a 31 amino acid, opioid neurotransmitter found in the neurons of the central and peripheral nervous system
Mouse anti Human Alkaline Phosphatase antibody, clone BGN/03/661 recognizes human alkaline phosphatase, a hydrolase enzyme most active at alkaline pH. Mouse anti Human Alkaline Phosphatase antibody, clone BGN/03/661 detects the tissue specific isotypes, BAP, PLAP and IAP (bone, placental and intestinal alkaline phosphatase), but may react with other alkaline phosphatases.
Mouse anti Human aggrecan antibody, clone 7E1 recognizes human aggrecan, a proteoglycan and member of the aggrecan/versican proteoglycan family, which forms a major component of the extracellular matrix (ECM) of both cartilage and the central nervous system (CNS).
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
70
Concentration:
IgG 1 mg/ml
Format:
Purified
Storage buffer:
PBS with <0.1% sodium azide
Storage:
aliquots -20°C. Thawed 2-8°C
References 1:
Virgintino D et al. J Cell Mol Med 2009; 13: 3151-73
Mouse anti Human chorionic gonadotrophin antibody, clone INN-hCG-22 recognizes the beta subunit of human choriogonadotrophin (hCG), also known as chorionic gonadotrophin. hCG? is a 165 amino acid ~18 kDa hormone involved in the stimulation of steroid production essential to the maintenance of pregnancy. Mouse anti Human chorionic gonadotrophin antibody, clone INN-hCG-22 shows a strong reaction in RIA with intact hCG and hCG? and some reactivity with human luteinizing hormone (12%) and b-hLH (34%). No reaction with human follicle-stimulating hormone, thyroid-stimulating hormone , a-hCG or a-hLH
Mouse anti hCG antibody, clone INN-hCG-5 recognizes an epitope on the alpha subunits of all human glycoprotein hormones. Indirect Immunofluorescence analysis on paraffin sections of human pituitaries reveals clear staining of glycoprotein hormone producing cells. Reacts with human LH, FSH, TSH, and hCG
202-36 Reacts with human CD26 (Dipeptidyl peptidase IV), a 110 kDa protein which occurs in the cell membrane of T-lymphocyte and is upregulated after activation. CD26 is also present on endothelial cells and also expressed on activated B-cells and natural killer cells and abundantly on epithelia. CD26 is implicated in a variety of biological functions including T-cell activation, cell adhesion with extracellular matrix such as fibronectin or collagens, and in HIV infection. Crosslinking of CD26 using this antibody dramatically enhances the anti-CD3-induced IL-2 production. In Western blotting, this mAb reacts with only glycosylated CD26, but not with deglycosylated froms. It does not prevent ADA binding to CD26. The mAb was clustered at the VIth WLDA.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
202-36
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Kishimoto T. et al., eds. Leukocyte Typing VI, Garland Publishing, (1997)
CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms – FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-154
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Bra4F1 reacts with CD15 (220 kDa). CD15 is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. CD15 is further expressed in Reed-Sternberg cells in classic Hodgkins disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes. It is also expressed on a wide variety of other tumor cells including myeloid leukemia, breast, colorectal, and lung cancer cells. Bra4F1 was clustered at the IVth International Workshop on Leucocyte Differentiation Antigens.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
BRA4F1
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Chorvath B. et al., Neoplasma 36(6), 633-642 (1989)
References 2:
Leukocyte typing IV, Oxford Univ. Press, 868, 870 and 877 (1989)
87-6F9 Reacts with CD11a, a transmembrane molecule with 1145 amino acid residues and a MW of 180 kDa. CD11a is expressed on lymphocytes, granulocytes, monocytes and macrophages. Levels on memory T-cells tend to increase. CD11a plays a key role in mediating leukocyte adhesion to endothelium during inflammatory response through binding to ICAM 1 (CD54). Other ligands are ICAM-2 and ICAM-3. It is also involved in many other T-cell functions and immune phenomena. When paired with CD18, it forms the integrin alphaLbeta2 adhesion. 87-6F9 Potently blocks LFA-1 dependent homotypic cell aggregation and was typed in the IVth International Leucocyte Typing Workshop
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
87-6F9
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Knapp et al. (Eds.), Leukocyte Typing IV, 1989 : sections N1 (pp. 543.551)
References 2:
Petruzzelli L et al. J Immunol 155(2): 854-866 (1995)
References 3:
Edwards CP et al. J Biol Chem 273(44): 28937-44 (1998)
References 4:
Shang XZ et al,. Eur J Immunol 28(6): 1970-1979 (1998)
CB-T6 specifically reacts with CD1a, an MHC like transmembrane glycoprotein of 49 kDa, that presents lipid molecules and is non-covalently associated with beta2-microglobulin. The antigen is strongly expressed on cortical thymocytes and dendritic cells and disappears at later stages of maturation.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
CBT6
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Crispe N. et al. Immunol. Today 17: 522-525 (1996)
References 2:
Yakimchuk et al. Eur J Immunol. 41(3): 694705 (2011). doi:10.1002/eji.201040808
Recognizes a 67kDa transmembrane protein, which is identified as CD5. The CD5 antigen is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. CD5 is expressed by many T cell leukemia, lymphomas, and activated T cells. Occasionally, CD5 antigen is also expressed on a subset of B cells. Mantle cell lymphomas (same as diffuse centrocyte lymphomas) are CD5+ while the follicle center cell lymphoma is CD5-negative. This MAb is superb for identifying the formalin-fixed, paraffin-embedded mantle cell lymphomas. Pretreatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0, for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based detection is recommended. Control tissue Tonsil. Staining Membranous.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
C5/473
Concentration:
n/a
Format:
Concentrate
Storage buffer:
Bioreactor Concentrate with 0.05% Azide
Storage:
2-8°C
References 1:
Ferry JA et. al. American Journal of Clinical Pathology, 1996, 105(1):31-7.
References 2:
Gagneten D et. al. Diagnostic Cytopathology, 1996, 14(1):32-7.
Signaling from the ligand-activated membrane receptor serine/threonine kinases to nuclear targets is mediated by a set of evolutionarily conserved proteins known as SMADs. Upon ligand binding, the receptors of the TGF-? family phosphorylate SMAD proteins (SMAD1 and SMAD2). These proteins then move into the nucleus, where they activate transcription. To carry out this function, the receptor activated SMAD 1 and 2 require association with the product of deleted in pancreatic carcinoma, locus 4 (DPC4), also known as SMAD4. SMAD4/DPC4 is also implicated as a tumor suppressor, since it is inactivated in more than half of pancreatic carcinomas and to a lesser extent in a variety of other cancers. The lack of SMAD4 expression is present in approximately 80% of cases of pancreatic adenocarcinoma, but rarely in endometrial (0%), colorectal (0%), ovarian (3%), lung (0%), breast (2%) adenocarcinomas, and malignant melanoma (4%). SMAD4 is an important marker for confirming a diagnosis of pancreatic adenocarcinoma. Patients with pancreatic adenocarcinomas with SMAD4 protein expression had significantly longer survival than SMAD4 negative patients. Pretreatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0, or in 50 mM Tris buffer pH9.5, for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based detection is recommended. Control tissue Pacreatic adenocarcinoma. Staining Nuclear.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
B-8
Concentration:
n/a
Format:
Purified
Storage buffer:
Purified antibody fraction from mouse anti-serum with 0.2% BSA and 15mM sodium azide
p63 is a homolog of the tumor suppressor p53. It is identified in basal cells in the epithelial layers of a variety of tissues, including epidermis, cervix, urothelium, breast and prostate. p63 was detected in nuclei of the basal epithelium in normal prostate glands; however, it was not expressed in malignant tumors of the prostate. As a result, p63 has been reported as a useful marker for differentiating benign from malignant lesions in the prostate, particularly when used in combination with markers of high molecular weight cytokeratins and the prostate-specific marker AMACR (P504S). p63 has also been shown to be a sensitive marker for lung squamous cell carcinomas (SqCC), with a sensitivity of ~90%. Specificity for lung SqCC, vs. lung adenocarcinoma (LADC), is approximately 80%. In breast tissue, p63 has been identified in myoepithelial cells of normal ducts. Pretreatment: Heat induced epitope retrieval in 10 mM citrate buffer, for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based detection is recommended. Control tissue Breast, Prostate, Prostate carcinoma or lung or bladder squamous cell carcinoma
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
TP63/11
Concentration:
n/a
Format:
Concentrate
Storage buffer:
Bioreactor Concentrate with 0.05% Azide
Storage:
2-8°C
References 1:
Yang A, et al. Mol Cell 1998;2:305-16
References 2:
Signoretti S, et al Am J Pathol 2000;157:1769-75
References 3:
Yang A, et al. Nature 1999;398:714-18
References 4:
Barbareschi M. et al. Am J Surg Pathol 2001 Aug;25(8);1054-60
References 5:
Werling RW, et al. Am J Surg Pathol 2003 Jan;27(1):82-90
Recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (7080kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction. Pretreatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0, for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based detection is recommended. Control tissue Uterus, blood cessels, smooth muscle or leiomyosarcoma. Staining Cytoplasmic
PAX8 antibody recognizes a protein of 62kDa, identified as PAX8. It is a member of the paired box (PAX) family of transcription factors. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas, and atypical thyroid adenomas. PAX8 is expressed in the thyroid (and associated carcinomas), non-ciliated mucosal cells of the fallopian tubes, and simple ovarian inclusion cysts, but normal ovarian surface epithelial cells. PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas. PAX8 antibody may be used as an additional immunohistochemical marker for renal epithelial tumors. Pre-treatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0 for 20 minutes, is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based detection is recommended. Control tissue Renal Cell Carinoma. Staining Nuclear
CD31 is a transmembrane glycoprotein, 130-140 kDa, also designated platelet-endothelium cell adhesion molecule = PECAM-1, belonging to the immunoglobulin super family. CD31 is ligand for CD38 and plays a role in thrombosis and angiogenesis. CD31 is strongly expressed in endothelial cells and weakly expressed in megakaryocytes, platelets, occasional plasma cells, lymphocytes (esp. marginal zone B-cells, peripheral T-cells) and neutrophils. Pre-treatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0 for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based detection is recommended. Control tissue Tonsil. Staining Membranous
p120 catenin is encoded on chromosome 11q11. Alpha-catenin and beta-catenin bind to the intracellular domain of E-cadherin while p120 catenin binds E-cadherin at a juxta-membrane site. The complex stabilizes tight junctions. In the cell, p120 catenin localized to the E-cadherin/catenins cell adhesion complex, directly associates with cytoplasmic C-terminus of E-cadherin and may similarly interact with other cadherins. A deficiency of E-cadherin results in the intracytoplasmic accumulation of p120 catenin. Lobular carcinoma of the breast shows intracytoplasmic accumulation of p120 catenin while ductal carcinoma shows reduced membrane p120 catenin without cytoplasmic accumulation. In gastric and colonic carcinoma, strong cytoplasmic p120 catenin is associated with discohesive infiltrative morphology.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MRQ-5
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Reynolds AB, et al. Oncogene.; 7:2439-45 (1992)
References 2:
Thoreson MA, et al. J Cell Biol.; 148:189-202 (2000)
References 3:
Sarrio D, et al. Oncogene.; 23:3272-83 (2004)
References 4:
Dabbs DJ, et al. Am J Surg Pathol.; 31:427-37 (2007)
Oct-4 is a transcription factor that functions in the regulation and maintenance of pluripotency in embryonic stem and primordial germ cells. Oct-4 immunoreactivity has been demonstrated in gonadal and extra-gonadal seminomas, dysgerminomas and embryonal carcinomas. In addition, the immunohistochemical detection of Oct-4 assists in the evaluation of intratubular germ cell neoplasia (IGCN).
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN
Clone:
MRQ-10
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Cheng L, et al. J Pathol. 2007; 211:1-9
References 2:
Weissferdt A, et al. Hum Pathol. 2015; 46:376-83
References 3:
Browne P, et al. Am J Clin Pathol. 2003 Nov;120(5):767-77
References 4:
García-Cosío M, et al. Mod Pathol. 2004 Dec;17(12):1531-8
References 5:
Gibson SE, et al. Am J Clin Pathol. 2006 Dec;126(6):916-24
Oct-2 is a transcription factor of the POU homeo-domain family that binds to the Ig gene octamer sites, regulating B-cell-specific genes. These are involved in proliferation and differentiation and, despite the scarce evidence for Oct-2 expression in T cells, it has been shown that this factor participates in transcriptional regulation during T-cell activation. Oct-2 activity is dependent on phosphorylation and alternatitive splicing.Various lymphomas are also positive for this marker including the following: Bchronic lymphocytic leukemia, mantle cell lymphoma, follicular lymphoma, marginal zone lymphoma, plasmacytoma, Burkitt lymphoma, diffuse large cell lymphoma, diffuse large B-cell lymphoma, T-cell rich B-cell lymphoma, nodular lymphocyte predominant Hodgkin lymphoma, and classic Hodgkin lymphoma.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MRQ-2
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Browne P, et al. Am J Clin Pathol. 2003; 120:767-77
References 2:
García-Cosío M, et al. Mod Pathol. 2004; 17:1531-8
References 3:
Gibson SE, et al. Am J Clin Pathol. 2006; 126:916-24
R-Ras2 / TC21 is the only member of R-Ras family of small GTPases that shows transforming activities similar to H-Ras, N-Ras, and K-Ras, and it is also structurally similar to them. R-Ras2 seems to play an important role in activating signal transduction pathways that control cell proliferation. Its mutations are associated with the growth of certain tumors, but also overexpression of the wild type form of R-Ras2 has been frequently detected in various carcinomas. Pseudogenes of R-Ras2 gene are found on chromosomes 1 and 2. Alternate splicing results in multiple transcript variants.SpecificityThe mouse monoclonal antibody NVL-07 recognizes intracellular part of human transmembrane adaptor SCIMP. This protein of 17 kDa predicted Mw migrates as a 22 kDa band on SDS PAGE.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml. Intracellular staining.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
EM-50
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Ribosomal protein SA (RPSA) is a multi-functional protein, that is an important component of 40S ribosomal subunit, and binds to lamin. Higher expression of RPSA is characteristic for many carcinomas, and correlates with their invasivity and metastatic potential. It has also been described, that RPSA interacts with amyloid beta peptide during Alzheimer´s disease.SpecificityThe mouse monoclonal antibody EM-50 recognizes R-Ras2 / TC21 protein (intracellular antigen) and does not react with R-Ras1, H-Ras, K-Ras, and N-Ras.Application detailsFlow cytometry: Recommended dilution: 8-12 ?g/ml. Intracellular staining.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
RP-01
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
RLTPR / CARMIL2 (RGD motif, leucine rich repeats, tropomodulin domain and proline-rich containing; capping protein regulator and myosin 1 linker 2), also known as LRRC16C, is a cytosolic protein, which with high affinity binds CAPZA2 (capping protein muscle actin Z-line alpha 2) and decreases CAPZA2 affinity for actin barbed ends. RLTPR / CARMIL2 increases the rate of actin filament elongation from seeds in the presence of CAPZA2, however, seems unable to nucleate filaments. Its interaction with CAPZA2 is essential for lamellipodial protrusion and cell translocation. RLTPR / CARMIL2 is crutial for T cell costimulation via CD28 and this property seems to be independent on its actin-uncapping function. The lack of functional RLTPR / CARMIL2 molecules impeded the differentiation toward Th1 and Th17 fates of both human and murine CD4+ T cells and leads to combined immunodeficiency. Expression of RLTPR / CARMIL2 was also detected in human and murine B cells, but it seems not to be involved in BCR-mediated signaling.SpecificityThe mouse monoclonal antibody RP-01 recognizes ribosomal protein SA (RPSA), which is important for formation and stability of 40S ribosomal subunit, and is overexpressed in many carcinomas.Application detailsFlow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
EM-53
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Glutamate carboxypeptidase II (GCPII), also known as N-acetyl-alpha-linked acidic dipeptidase I (NAALADase I), folate hydrolase (FOLH1), and prostate-specific membrane antigen (PSMA), is an approximately 95-110 kDa type II transmembrane glycoprotein expressed in various tissues. In nervous system GCPII cleaves abundant N-acetylaspartylglutamate, which is released from neurons in a calcium-dependent manner, to N-acetylaspartate and glutamate. As immoderate glutamate concentration is neurotoxic, GCPII contributes to pathological conditions regarding e.g. Alzheimer´s disease, Huntington´s disease, epilepsy, schizophrenia, stroke or neuropathic pain and appears to be an interesting therapeutic target. In jejunum GCPII hydrolyzes pteroylpoly-gamma-glutamate to folate and glutamate, enabling folate to be absorbed by gastrointestinal tract. GCPII, which is present in a number of tissues at low levels, is overexpressed in neovasculature of most solid tumours and is a target enzyme for diagnosis and treatment of prostate cancer. Normal human prostate express more mRNA coding for a cytosolic GCPII form truncated at the N-terminus (PSM´) than mRNA for membrane-bound GCPII, and this ratio is reversed upon malignant transformation.SpecificityThe mouse monoclonal antibody EM-53 recognizes RLTPR / CARMIL2, an intracellular protein playing a role in actin filament elongation.Application detailsWestern blotting: Recommended dilution: 1 ?g/ml; positive control: LNCaP cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold lysis buffer (1% NP-40). Incubate 30 min on ice. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer. Both reducing and non-reducing conditions.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
GCP-04
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Prostate-specific antigen (PSA) is a protein produced by normal prostate cells. This enzyme participates in the dissolution of the seminal fluid coagulum and plays an important role in fertility. The highest amounts of PSA are found in the seminal fluid; some PSA escapes the prostate and can be found in the serum. This serum component has been used to track the response to therapy in men with prostate cancer.SpecificityThe mouse monoclonal antibody GCP-04 recognizes amino acids 100-104 of extracellular domain of denaturated glutamate carboxypeptidase II (PSMA, NAALADase, FOLH1), an approximately 95-110 kDa transmembrane glycoprotein.Application detailsWestern blotting: Positive material: seminal plasma, reducing conditions, recommended antibody dilution: 2-5 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
A67-B/E3
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
PRR7/TRAP3 (proline-rich 7, transmembrane adaptor protein 3) is a 28 kDa transmembrane adaptor protein ubiquitously expressed at low level (most in brain). Its amino acid sequence is extremely conserved among mammalian and other species. PRR7/TRAP3 contains potential palmitoylation motif and is found in lipid rafts. It is a part of the complex postsynaptic density fraction in neurons and associates with PSD-95, NMDA receptor and probably other proteins. The intracellular domain of PRR7/TRAP3 contains several tyrosines, a proline-rich sequence, and a C-terminal PDZ-binding motif. So far nothing is known about function of this protein. It may be involved in regulation of some receptor signaling and in formation of neurologic and immunologic synapse.SpecificityThe mouse monoclonal antibody A67-B/E3 reacts with prostate-specific antigen (PSA), a protein produced by normal prostate cells. The highest amounts of PSA are found in the seminal fluid. This antibody recognizes both free and complexed PSA.Application detailsImmunocytochemistry: Recommended dilution: 10 ?g/ml; cell culture fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton-X100. <br>Western blotting: Recommended dilution: 1 ?g/ml; positive control: murine brain lysate (red. Laemmli buffer).
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
TRAP3/10
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
PRKAR2A (proteinkinase A regulatory type II alpha subunit), also known as PKR2, or PRKAR2, is a component of cAMP-dependent protein kinase complex. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The PRKAR2A subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER). In sperm, this antigen can be used as an intra-acrosomal marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.SpecificityThe mouse monoclonal antibody TRAP3/10 recognizes an epitope located in the C-terminal part of the intracellular domain of PRR7/TRAP3 (amino acids 126-253 of human PRR7 / TRAP3), a 28 kDa proline-rich membrane protein presumably associated with NMDA receptor complex.Application detailsImmunocytochemistry: Recommended dilution: 10 ?g/ml; membrane permeabilization (acetone) is essential.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
Hs-36
Concentration:
1 mg/ml
Format:
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Storage buffer:
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
PPM1D is a Mg2+/Mn2+ dependent protein phosphatase 1D with inducible expression in response to various types of environmental stress. This expression is p53-dependent, and subsequently PPM1D negatively regulates the p53-mediated transcription, thus it suppresses the apoptosis. PPM1D contributes to development of carcinomas, and seems to be a promissing therapeutic target. Amplification of PPM1D is associated with breast cancer.SpecificityThe antibody Hs-36 reacts with PRKAR2A (protein kinase A regulatory type II alpha subunit), an intra-acrosomal protein.Application detailsWestern blotting: Recommended dilution: 1-2 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
7E11/C5
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Perforin is a 70 kDa cytolytic protein with structural and functional similarities to complement component 9 (C9). It is stored in cytoplasmic granules of cytotoxic T cells and NK cells and after its release it forms transmembrane pores in the target cells to kill it. As perforin is a key effector molecule for cell-mediated cytolysis, defects of its gene can cause severe disorders.SpecificityThe mouse monoclonal antibody 7E11/C5 recognizes an epitope within the sequence FTNEDELYNLLTDSP of PPM1D, a protein phosphatase, which contributes to development of some carcinomas.Application detailsFlow cytometry: Recommended dilution: 3-12 ?g/ml. Intracellular staining.
Antibody Isotype:
IgG2b kappa
Monosan Range:
MONOSAN
Clone:
dG9
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
PCNA (proliferating cell nuclear antigen) is the DNA polymerase delta auxiliary protein acting in homotrimeric form to increase the processivity of leading strand synthesis during DNA replication. PCNA is expressed in the nucleus of all proliferating cells. In response to DNA damage, it is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. PCNA is a useful marker of DNA synthesis, as its form not involved in DNA synthesis degradates in histological preparations in the presence of organic solvents.SpecificityThe mouse monoclonal antibody dG9 (also known as deltaG9) recognizes perforin, a 70 kDa protein expressed in cytoplasmic granules of cytotoxic T cells and NK cells.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml. Intracellular staining.
Antibody Isotype:
IgG2a kappa
Monosan Range:
MONOSAN
Clone:
PC10
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
PCLO (piccolo, also known as aczonin) is a large (more than 400 kDa) multidomain protein of the presynaptic cytomatrix in neurons, that is present in all vertebrate synapses, but is absent from invertebrates. It contains zinc finger and coiled-coil sequences, as well as N-terminal glutamine-rich sequence, and C-terminal PDZ domain followed by two C2 domains (C2A and C2B). In vitro binding and transfection experiments suggested that PCLO binds to multiple proteins including profilin and L-type calcium channels. It is involved in neurotransmitter release and insulin secretion.SpecificityThe mouse monoclonal antibody PC10 (also known as 3F81) recognizes PCNA, a 36 kDa conserved nuclear protein serving as a cofactor for DNA synthesis.Application detailsFlow cytometry: Recommended dilution: 1-5 ?g/ml, intracellular staining.<br>Western blotting: A small splice variant of PCLO can be analyzed by Western blotting, however, the whole protein is too large (over 400 kDa) to be successfully processed by SDS PAAGE.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
PCLO-01
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
The p53 family of proteins includes three members, p53, p63, and p73. The protein p63 is encoded by TP63 gene, which gives rise to protein isoforms with different properties and functions due to the presence (TAp63) or absence (deltaNp63) of an N-terminal transactivation domain. Immunohistochemistry of p63 has a clinical relevance for certain tumor types, but investigations have been hampered by a lack of well characterized antibodies that are specific for p63 and do not cross-react with the related p53 and p73 proteins.SpecificityThe mouse monoclonal antibody PCLO-01 recognizes PCLO (Piccolo), a more than 400 kDa multidomain protein expressed mainly in the presynaptic cytoplasmatic matrix of the neurons.Application detailsWestern blotting: Recommended dilution: 1 ?g/ml
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
PANp63-6.1
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
The tumour suppressor p21Waf1 (wild-type p53-activated fragment 1; also known as Cip1, Cdk interacting protein, or SDI 1) is a cyclin-dependent kinase (Cdk) inhibitor, which is expressed by involvement of p53, Egr-1, AP2, STATs or other transcription factors upon various stimuli resulting in cell cycle arrest. Through its N-terminal domain p21Waf1 inhibits Cdk activity, whereas through the C-terminal domain it inhibits the activity of PCNA (proliferating cell nuclear antigen) to activate DNA replication. Cytosolic location of p21 counteracts its inhibitory activities.SpecificityThe mouse monoclonal antibody PANp63-6.1 recognizes both TAp63, and deltaNp63 form of p63. The target epitope PSHLIR is located within amino acids 261-266 of TAp63, and 167-172 of deltaNp63.Application detailsImmunohistochemistry (paraffin sections): Positive tissue: colon carcinoma.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
WA-1
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
OPAL1 (outcome predictor in acute leukemia 1) is an almost uncharacterized transmembrane adaptor protein expressed mainly by megacaryocytes and platelets. Its expression is enhanced on TEL/AML leukemic cells. The function of OPAL1 is unknown, although the presence of a cytochrome c-like heme-binding site and a transmembrane domain suggested OPAL1 may be involved in the mitochondrial electron transport chain. Its originally reported prognostic impact appears to be treatment dependent.SpecificityThe antibody WA-1 reacts with p21 protein (Waf1, Cip1, SDI 1; intracellular antigen), a 21 kDa tumour suppressor, which inhibits the activity of cyclin-dependent family kinases and of proliferating cell nuclear antigen.Application detailsFlow cytometry: Recommended dilution: 1-4 µg/ml, intracellular staining.<br>Western blotting: Recommended dilution: 1-2 ?g/ml, non-reducing conditions.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
OPAL1-01
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
NR2F6 (nuclear receptor subfamily 2 group F member 6), also known as EAR2 or ERBAL2, is a transcription factor involved in modulation of hormonal responses. NR2F6 represses e.g. transcription of the oxytocin-neurophysin gene, renin gene, lutropin-choriogonadotropic hormone receptor gene, and the thyroid hormone receptor gene. In the immune system, NR2F6 affects IL-17 expression in the Th-17 cells, thus also the balance between immunological tolerance and autoimmunity.SpecificityThe mouse monoclonal antibody OPAL1-01 recognizes an intracellular epitope of OPAL1 (outcome predictor in acute leukemia 1), a transmembrane adaptor protein expressed mainly by megacaryocytes and platelets.Application detailsWestern blotting: Positive control: HEK293T/17/NR2F6/Ha-tag transfectants, negative control: HEK293T/17 cells. <br>Flow cytometry: Recommended dilution: 3-9 µg/ml. Intracellular staining.
Antibody Isotype:
IgG2a kappa
Monosan Range:
MONOSAN
Clone:
EM-51
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Notch 1 is a 270-300 kDa transmembrane heterodimeric protein with multiple extracellular growth factor-like repeats, and with an intracellular domain consisting of multiple different domain types. It serves as a receptor for membrane ligands, such as Delta 1, Jagged 1 (CD339), and Jagged 2, and regulates cell fate decisions. Upon ligand binding the transmembrane form of Notch 1 is repeatedly cleaved to provide approximately 120 kDa Notch intracellular fragment (NICD), which translocates to the nucleus and acts as a part of transcriptional complexes that alter differentiation, proliferation, and apoptosis. The highest level of Notch 1 expression is in brain, lung and thymus.SpecificityThe mouse monoclonal antibody EM-51 recognizes NR2F6, a transcriptional repressor (intracellular antigen) expressed mainly in the heart, placenta, liver, skeletal muscle, kidney and pancreas, but also e.g. in T cell subpopulations.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml. Intracellular staining.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
mN1A
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
NG2 / chondroitin sulfate proteoglycan 4 is expressed on glial cell populations, but not on normal hepatopoietic cells. It is an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells, where it plays role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes, and supports signaling pathways important for tumor invasion and growth. NG2 also serves as an AML blast tumor marker associated with poor prognosis.SpecificityThe mouse monoclonal antibody mN1A recognizes intracellular domain of Notch 1 protein, mainly its activated form. The unprocessed Notch 1 protein is recognized with lower affinity.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
7.1
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs in most vertebrates are composed of three different polypeptide chains with different molecular weights – neurofilament heavy protein (NF-H), medium (NF-M) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. The protein segment on the C-terminal side of the human NF-H rod is uniquely long (more than 600 amino acids) compared to other IF proteins and is highly charged (> 24 % Glu, > 25 % Lys), rich in proline (> 12 %) and improverished in cysteine, methionine and aromatic amino acids. Its most remarkable feature is a repetitive sequence that covers more than half its lenght and includes the sekvence motif Lys-Ser-Pro (KSP) greater than 40 times. Plasma neurofilament heavy chain level has been proposed as a marker of axonal injury and clinical use of its degeneration and loss has been suggested as a biomarker of several neurodegenerative diseases.SpecificityThe mouse monoclonal antibody 7.1 recognizes an extracellular epitope of NG2, the melanoma-associated chondroitin sulfate proteoglycan 4 of Mw approximately 220-300 kDa.Application detailsELISA: Capture antibody. <br>Western blotting: Recommended dilution: 1-2 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
NF-05
Concentration:
1 mg/ml
Format:
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
NCK1 (NCK alpha) is a cytoplasmic adaptor protein that plays a universal role in coordinating the signaling networks critical for organizing the actin cytoskeleton, cell movement, or axon guidance, connecting transmembrane receptors to multiple intracellular signaling pathways. It contains one SH2 domain, through which NCK1 binds to phosphorylated domains of transmembrane signaling moleculs or certain adaptor proteins, and three SH3 domains for binding proline-rich sequences of other molecules involved in the process of nucleation and polymerization of the actin cytoskeleton.SpecificityThe antibody NF-05 recognizes a nonphosphorylated epitope of neurofilament heavy protein (NF-H), a 210 kDa intracellular structural protein of Intermediate Filament Proteins family. NF-H is mainly expressed in the central and peripheral nervous system and reproductive system and is biochemically very stable.Application detailsWestern blotting: Recommended dilution: 2 ?g/ml; positive control: Jurkat cell lysate. <br>Immunocytochemistry: Recommended dilution: 2 ?g/ml; positive control: human NCK1-transfected COS-7 cells.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
EM-06
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
MICA and MICB glycoproteins are members of MHC class I family, closely linked to HLA-B. However, unlike HLA molecules, MICA and MICB are not associated with beta2 microglobulin and are conformationally stable in the absence of conventional MHC class I peptide ligands. Both proteins are stress-induced antigens expressed mainly in gastrointestinal epithelium, where they are recognized by V-delta1 subset of gamma/delta T cells, and also on diverse epithelial tumor cells. Binding of MICA/MICB receptor, the NKG2D, leads to cytolytic response of NK cells, Tc cells, and gamma/delta T cells. Alternative splicing results in multiple isoforms, and some of them have been associated with susceptibility to psoriasis and psoriatic arthritis. Shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma.SpecificityThe mouse monoclonal antibody EM-06 recognizes NCK1 (NCK alpha), an ubiquitously expressed cytoplasmic SH2/SH3 adaptor protein important for organization of actin cytoskeleton structures.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
6D4
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
MAP2a and 2b (270 kDa) being found mostly in dendrites, stabilize microtubules (shift the reaction kinetics in addition of new subunits and microtubule growth) and participate in determining the structure of different parts of vertebrate nerve cells.SpecificityThe mouse monoclonal antibody 6D4 recognizes a common extracellular epitope on MICA and MICB glycoproteins, transmembrane ligands of NKG2D, and is able to block NKG2D-mediated activation of NK cells and cytotoxic T cells.Application detailsImmunohistochemistry (paraffin sections): Recommended dilution: 10 ?g/ml; positive tissue: brain. <br>Immunohistochemistry (frozen sections): Positive tissue: murine brain. <br>Immunoprecipitation: Positive material: porcine brain. <br>Western blotting: Positive control: porcine brain. <br>ELISA: Positive control: porcine brain.<br> Immunocytochemistry: Positive control: human neuroblastoma SH-SY5Y.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MT-07
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Lysozyme is anti-bacterial enzyme found mainly in milk, saliva, tears, plasma, spleen, mucus, and leukocytes (e.g. in cytoplasmic granules of neutrophils). It damages bacterial cell walls by hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins. Lysozyme is part of the innate immune system. It protects wet body surfaces, such as conjunctiva. Reduced lysozyme levels have been associated with bronchopulmonary dysplasia in newborns. On the other hand high lysozyme blood levels produced for example by myelomonocytic leukemia cells can lead to kidney failure and low blood potassium.SpecificityThe antibody MT-07 recognizes an epitope (aa 1375-1395) located in central domain of molecule Microtubule Associated Protein 2ab (MAP2ab), an intracellular antigen.Application detailsFlow cytometry: Intracellular staining; recommended dilution: 1-12 µg/ml. Excellent. <br>Immunohistochemistry (paraffin sections): Positive tissue: tonsil.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
LZ598-10G9
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
LST1 (leukocyte-specific transcript 1, also known as B144) is expressed in cells of myeloid/erythroid lineage (monocytes, granulocytes, dendritic cells, platelets, erythrocytes). At least 14 alternatively spliced variants (LST1/A – LST1/N) can be detected; some of them (LST1/A, B, C, G, I, K) are transmembrane cell surface-exposed forms, the other are soluble. LST1 induces production of long, thin filopodia in dendritic cells, has an inhibitory effect on lymphocyte proliferation and may have an immunomodulatory role. LST1/A is an 11 kDa transmembrane adaptor present in membrane rafts and forms spontaneously covalent homodimers. Its intracellular domain contains two tyrosine motifs, one of them being an ITIM very similar to such motifs in Siglec.SpecificityThe mouse monoclonal antibody LZ598-10G9 recognizes lysozyme, an approximately 17 kDa antibacterial enzyme, which is being used as a marker for the lineage diagnosis of acute leukemias (intracellular antigen).Application detailsImmunoprecipitation: Positive control: RAJI human Burkitt lymphoma cell line. <br>Western blotting: The antigen migrates on SDS PAGE gels as approximately 25-28 kDa molecule.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
LST1/02
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
LARGE1 serves as a glycosyltransferase which participates in glycosylation of the muscle membrane protein alpha-dystroglycan. Mutations of LARGE1 lead to hypoglycosylation of alpha-dystroglycan and cause congenital muscular dystrophy (MDC1D) associated with severe mental retardation. Altered alpha-dystroglycan glycosylation may also play a role in cancer, as hypoglycosylation of the protein and loss of laminin binding have been demonstrated in invasive carcinoma cells.SpecificityThe antibody LST1/02 reacts with an extracellular epitope of LST1, an approximately 6-11 kDa protein expressed as various transmembrane or soluble forms. LST1 is found predominantly on monocytes and dendritic cells. It migrates on SDS PAGE gels as approximately 25-28 kDa molecule.Application detailsFlow cytometry: Recommended dilution: 1-5 ?g/ml. Intracellular staining.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
LARGE-02
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Lactoferrin is an iron-binding glycoprotein of the transferrin family, which is released to most biological fluids, with particularly high levels in milk. It has anti-inflammatory (e.g. sequestering of lipopolysaccharides), anti-microbial (e.g. blocking of viral attachment to the target cell), and immunomodulatory properties and can prevent infections in young children. Lactoferrin is considered to bridge the innate and adaptive immune responses. It also participates in iron homeostasis, regulation of cellular growth and differentiation and protection against cancer development and metastasis. Besides biological fluids it is also found in the secondary granules of neutrophils.SpecificityThe mouse monoclonal antibody LARGE-02 recognizes human LARGE1, a glycosyltransferase expressed mainly in the Golgi apparatus. Crossreactivity with LARGE2 was not determined.Application detailsFlow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
LF5-1D2
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Ku Antigen (DNA-dependent DNA helicase) is a heterodimer (of 72 and 87 kDa polypeptides) which contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the nonhomologous end-joining pathway. A DNA double-strand break is a major lesion that destroys the integrity of the DNA molecule. Such damage is introduced by ionizing radiation. Ku binds to free double-stranded DNA ends and is the DNA-binding component of the DNA-dependent protein kinase. Thus, the Ku protein is involved in DNA repair and in V(D)J recombination, and the Ku-DNA-dependent protein kinase complex may have a role in those same processes. Ku70 and Ku80 share a common topology and form a dyad-symmetrical molecule with a preformed ring that encircles duplex DNA. The binding site can cradle 2 full turns of DNA while encircling only the central 3-4 base pairs. Ku makes no contacts with DNA bases and few with the sugar-phosphate backbone, but it fits sterically to major and minor groove contours so as to position the DNA helix in a defined path through the protein ring. These features are well designed to structurally support broken DNA ends and to bring the DNA helix into phase across the junction during end processing and ligation. Mouse cells deficient for Ku80 display a marked increase in chromosomal aberrations, including breakage, translocations, and aneuploidy. Despite the observed chromosome instabilities, Ku80 -/- mice have only a slightly earlier onset of cancer. Loss of p53 synergizes with Ku80 to promote tumorigenesis such that all Ku80 -/- and p53 -/- mice succumb to disseminated pro-B-cell lymphoma before 3 months of age. The p70/p80 complex binds to the ends of double-stranded DNA in a cell cycle-dependent manner, being associated with chromosomes of interphase cells, followed by complete dissociation from the condensing chromosomes in early prophase. Some patients with systemic lupus erythematosus produce very large amounts of autoantibodies to p70 and p80. The autoantibody has been found in patients with autoimmune thyroid disease (Graves disease) as well as in those with lupus.SpecificityThe mouse monoclonal antibody LF5-1D2 recognizes lactoferrin, an iron-binding secreted glycoprotein of about 90 kDa, which has anti-inflammatory, immunomodulatory and anti-microbial properties.Application detailsImmunocytochemistry: Recommended dilution: 2 ?g/ml, PFA-fixation possible.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
MEM-54
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Kinesin belongs to the group of microtubule-associated motor proteins known to convert chemical energy released from nucleoside triphosphates (preferentially from ATP) into mechanical energy. Conventional kinesin, member of the kinesin superfamily comprising more than 100 proteins, is involved in the anterograde vesicle transport in neuronal cells. Kinesin purified from mammalian brain homogenates is a heterotetramer consisting of two heavy (120 to 130 kDa) and two light chains (60 to 70 kDa), resulting in a molecular mass about 400 kDa. Each heavy chain contains an N-terminal globular motordomain with both a microtubule-binding site and an ATPase active center, stalk region responsible for heavy chain dimerization and finally C-terminal globular tail domain, which is implicated in cargo binding. Light chains may have a regulatory function.SpecificityThe antibody MEM-54 reacts with Ku80, a 80 kDa subunit of Ku autoantigen (heterodimer of 72 and 87 kDa intracellular polypeptides). Ku autoantigen is involved in DNA repair and in V(D)J recombination through its ability to bind DNA double-strand breaks.Application details
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
KN-02
Concentration:
1 mg/ml
Format:
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Storage buffer:
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Ki-67 is a highly protease-sensitive nuclear protein expressed in two isoforms (345 kDa and 395 kDa), both of which are identified by the antibody clone Ki-67. The Ki-67 antigen is essential for cell proliferation and its expression is restricted to the cycling cells. It is detected in G1, S, G2 and M phase, whereas it is absent in cells which are in G0 phase and it is not associated with DNA repair processes. Ki-67 thus represents an important tool for detection of proliferating cells, which is of great importance in tumor diagnostics and is commonly used as a prognostic factor in cancer studies.SpecificityThe antibody KN-02 recognizes heavy chain of conventional kinesin, a protein associated with intracellular vesicles, and with lower affinity with denaturated molecule. Epitope is located in coiled-coil stalk domain. It stains Western blots of kinesin-enriched preparations. Epitope mapping (by limited proteolysis of partially purified porcine kinesin) followed by immunoblotting has revealed that antibodies KN-01, KN-02 and KN-03 react with different sets of fragments. The antibody KN-02 does not react with kinesin bound to taxol-stabilized microtubules.Application detailsFlow cytometry: Recommended dilution: 1-12 µg/ml. Intracellular staining.<br>Immunocytochemistry: Paraformaldehyde fixation; recommended antibody concentration 1 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
Ki-67
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Ikaros, also known as IKZF1 (Ikaros family zinc finger protein 1) is a hematopoietic-specific transcription factor involved in the regulation of lymphocyte development, together with other members of this family, such as Aiolos and Helios. Ikaros forms homo- and heterodimers with these proteins and functions predominantly in early hematopoietic development. Expression of Ikaros, Aiolos and Helios is restricted to cells of the hematopoietic system, whereas other family members, Eos and Pegassus, are more widely expressed. Disruption of Ikaros leads to T and B cell leukemias.SpecificityThe mouse monoclonal antibody Ki-67 recognizes Ki-67 antigen, a non-histone nuclear protein expressed exclusively in proliferating cells.Application detailsFlow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
4E9
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
The interferon gamma (IFN-gamma; 16-25 kDa) is an important regulator of the immune response, produced in activated Th1 cells and NK cells, particularly in response to IL-2, TNF-alpha and IL-12; its production is suppressed by IL-4, IL-10, and TGF-beta. The producing of IFN-gamma is activated by specific antigens or mitogens through the T cell antigen receptor. IFN-gamma polypeptide forms: 40-60 kDa forms are observable under non-denaturing conditions as dimers and trimers; 20 kDa and 25 kDa forms exist due to variable glycosylation. IFN-gamma belongs to the type II interferons, also called immune IFN. IFN-gamma shows antiviral activity and has important immunoregulatory functions. It is a potent activator of macrophages and had antiproliferative effects on transformed cells. IFN-gamma plays an important role in regulating B cell differentiation by simultaneously stimulating class switch recombination to the IgG3 and IgG2a isotypes while represing class switch recombination to the IgE and IgG1 isotypes. It also appears to promote antigen presentation by B cells through its effects on MHC. Binding of IFN-gamma to its receptor increases the expression of class I MHC on all somatic cells. It also enhances the expression of class II MHC on antigen-presenting cells. IFN-gamma is the major means by which T cells activate macrophages, increasing their ability to kill bacteria, parasites, and tumours. The activation of macrophages by IFN-gamma is essential for the elimination of bacteria that replicate within the phagosomes of macrophages (f.e. Mycobacteria and Listeria monocytogenes). IFN-gamma can potentiate the high antiviral and antitumor effects of the type I interferons (IFN-alpha, IFN-beta). IFN-gamma may also activate neutrophils and NK cells.SpecificityThe mouse monoclonal antibody 4E9 recognizes Ikaros, a transcription factor (intracellular antigen) expressed broadly in hematopoietic progenitors and serving as a key regulator of lymphopoiesis.Application detailsFlow cytometry: Intracellular staining; recommended dilution: 2-4 ?g/ml; positive control: PMA/ionomycin stimulated peripheral blood lymphocytes (PBL). <br>Western blotting: Recommended dilution: 1 ?g/ml; positive control: recombinant human IFN-gamma.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
G-23
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
TROP2 is a cell surface receptor that transduces calcium signals. It belongs to carcinoma-associated antigens. Mutations of TROP2 have been associated with gelatinous drop-like corneal dystrophy.SpecificityThe mouse monoclonal antibody G-23 reacts with IFN-gamma, a 16-25 kDa cytokine produced by activated Th1 cells and NK cells.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.<br>Western blotting: Recommended dilution: 1 ?g/ml.<br>Immunocytochemistry: Recommended dilution: 10 ?g/ml; the cells can be fixed with 4% PFA and permeabilized with 0.1% Triton X-100 before antibody staining, when needed. <br>Immunohistochemistry (paraffin sections): Recommended dilution: 10 ?g/ml. Heat-mediated antigen retrieval in citrate buffer, pH = 6.
Antibody Isotype:
IgG2b kappa
Monosan Range:
MONOSAN
Clone:
TrMab-6
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
TCR Vgamma9 is a variant of TCR gamma chain, that is present on a subset of human gamma/delta T cells. TCR Vgamma9/Vdelta2 T cells are able to recognize and kill various tumor cells, as this receptor heterodimer binds to certain phosphoantigens, expressed by tumors.SpecificityThe mouse monoclonal antibody TrMab-6 recognizes an extracellular epitope of TROP2, a type I transmembrane glycoprotein. This antibody is usefull for detection of TROP2 in breast cancer.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
B3
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
TCR Vgamma4 is a variant of TCR gamma chain, that is present on a minor subset of human gamma/delta T cells.SpecificityThe mouse monoclonal antibody B3 recognizes an extracellular epitope of human TCR Vgamma9, a variant of TCR gamma chain, which is expressed on a gamma/delta T cell subpopulation with cytolytic activity against various tumor cells.Application detailsFlow cytometry: Recommended dilution: 2-6 ?g/ml
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
4A11.904
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
TCR Vdelta2 is a variant of TCR delta chain, that is present on a major subset of human gamma/delta T cells. TCR Vgamma9/Vdelta2 (or Vgamma2/Vdelta2) T cells are able to recognize and kill various tumor cells, as this receptor heterodimer binds to certain phosphoantigens, expressed by tumors. They can recognize these antigens in an MHC-unrestricted manner. Similarly to NK cells, Vdelta2 T cells express MHC I receptors and killer Ig-like receptors, that are involved in tumor recognition and cytolysis. The potently cytotoxic subset of them is identified by cell surface expression of polysialyated CD56.SpecificityThe mouse monoclonal antibody 4A11.904 recognizes an extracellular epitope of human TCR Vgamma4, that is present on a minor subset of human gamma/delta T cells.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
B6
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.SpecificityThe mouse monoclonal antibody B6 recognizes an extracellular epitope of human TCR Vdelta2, which is expressed on the majority of gamma/delta T cells.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
B1
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Alpha-beta T cell receptors (TCRs) are antigen specific receptors, which are essential to the immune response and are present on the cell surface of T lymphocytes. They recognize peptide-loaded major histocompatibility complexes (pMHCs), that are displayed by antigen presenting cells (APCs). Binding of alpha-beta TCR to pMHC initiates TCR-CD3 clustering on the cell surface and intracellular activation of LCK, that phosphorylates the ITAM motifs of CD3gamma, CD3delta, CD3epsilon and CD3zeta, enabling the recruitment of ZAP70. In turn, ZAP70 phosphorylates LAT, which recruits numerous signaling molecules to form the LAT signalosome. The LAT signalosome propagates signal branching to three major signaling pathways, the calcium signaling, the mitogen-activated protein kinase (MAPK) kinase and the nuclear factor NFkappaB (NF-kB) pathways, leading to the mobilization of transcription factors, that are critical for gene expression and essential for T cell growth and differentiation. The T cell repertoire is generated by V-D-J-C rearrangements. This repertoire is then shaped by intrathymic selection events to generate a peripheral T cell pool of self-MHC restricted, non-autoaggressive T cells. Post-thymic interaction of alpha-beta TCRs with the pMHCs shapes TCR structural and functional avidity.SpecificityThe mouse monoclonal antibody B1 (also known as B1.1) recognizes an extracellular epitope of TCR gamma/delta, the subtype of T cell receptor expressed mainly in epithelial tissues and at the sites of infection.Application detailsFlow cytometry: Recommended dilution: 2-6 µg/ml
Antibody Isotype:
IgG2a kappa
Monosan Range:
MONOSAN
Clone:
JOVI.1
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.SpecificityThe mouse monoclonal antibody JOVI.1 recognizes an extracellular epitope on TCR Cbeta1 (TRBC1).Application detailsFlow cytometry: Recommended dilution: 2-4 ?g/ml; positive control: human peripheral blood T cells.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
IP26
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
SUSD2 (sushi domain containing protein 2) is a type I transmembrane protein, that serves as an important marker of bone marrow-derived mesenchymal stem-like cells (bone marrow stromal cells). These pluripotent cells are important for techniques of autologous cell therapy, and can be collected from e.g. endometrium, or palatine tonsil. SUSD2 seems to be a tumor supresor, and is down-regulated in colon cancer tissues, whereas it is highly expressed e.g. in breast cancer.SpecificityThe mouse monoclonal antibody IP26 recognizes a monomorphic extracellular determinant of TCR alpha/beta, the dominant subtype of T cell receptor expressed in human peripheral blood.Application detailsFlow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
W5C5
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
STRO-1 is a cell surface antigen expressed by stromal elements in human bone marrow, identified by monoclonal antibody STRO-1. Approximately 10% of mononuclear cells, greater than 95% of which are nucleated erythroid precursors, are STRO-1 positive, whereas the CFU-GM (colony-forming unit granulocyte-macrophage), BFU-E (erythroid burst) and CFU-Mix (mixed colonies) committed progenitor cells are negative. CFU-F (fibroblast colony-forming cells) are present exclusively in the STRO-1 positive population. When plated under long-term bone marrow culture conditions, STRO-1 positive cells generate adherent cell layers containing multiple stromal cell types, including adipocytes, smooth muscle cells, osteoblasts, chondrocytes, and fibroblastic elements. In combination with glycophorin A, STRO-1 is a useful marker for identification of mesenchymal stem cells. STRO-1 and CD117 are markers for osteosarcoma cells.SpecificityThe mouse monoclonal antibody W5C5 recognizes an extracellular epitope of SUSD2, a type I transmembrane protein expressed on mesenchymal stem-like cells. This antibody selectively binds to a MSCs in both bone marrow and endometrium or tonsil, and can be used for their identification and isolation.Application detailsFlow cytometry: Recommended dilution: 1-5 ?g/ml.
Antibody Isotype:
IgM lambda
Monosan Range:
MONOSAN
Clone:
STRO-1
Concentration:
1 mg/ml
Format:
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Storage buffer:
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
STAT1 (signal transducer and activator of transcription 1) is a transcription factor that plays important roles in growth arrest, apoptosis promoting and tumour suppression. After ligation of cytokine receptors STAT1 becomes phosphorylated on Tyr701 by Janus kinase JAK1 or JAK2, dimerizes, translocates to nucleus and contacts DNA. STAT1-STAT2 heterodimers serve as more potent transcriptional inducers than STAT1 homodimers. STAT1 is also phosphorylated on Ser727 by MAPK pathway, independently of tyrosine phosphorylation. However, the both modifications are important for its maximal transcriptional activity. On the other hand, STAT1 phosphorylated on Ser727 is targeted for proteasomal degradation.SpecificityThe mouse monoclonal antibody STRO-1 recognizes an extracellular epitope of the cell surface antigen STRO-1 expressed by bone marrow mesenchymal stromal cells and nucleated erythroid precursors, but not by committed hematopoietic progenitors.Application details
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
PSM1
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
SIGLEC8 is a sialic acid binding lectin similar to CD33. In its cytoplasmic comain it contains an immunoreceptor tyrosine-based inhibitory motif (ITIM), and a motive similar to a binding site for SLAM-associated protein. SIGLEC8 is expressed e.g. in lymph nodes and spleen. It is an eosinophil marker, although it can be found also on the surface of mast cells. Crosslinking of SIGLEC8 leads to apoptosis. Soluble form of SIGLEC8 can be foud in human serum, especially in case of eosinophil-associated diseases.SpecificityThe antibody PSM1 recognizes transcriptional factor STAT1 (91 kDa) activated by phosphorylation at Ser727 (intracellular antigen).Application detailsFlow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
7C9
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
PODXL is a highly glycosylated sialomucin, which is expressed in many types of tumors, as well as it is a well known marker of embryonic stem cells. Overexpression of PODXL is an independent predictor of cancer progression, metastasis, and poor outcome. PODXL promotes tumor growts and invasiveness, and is a potential target for antibody therapy.SpecificityThe mouse monoclonal antibody 7C9 recognizes an extracellular epitope of SIGLEC8, an eosinophil marker, expressed e.g. in lymph nodes and spleen.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
PcMab-47
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
PDPN (podoplanin) is a type I transmembrane glycoprotein of mucin-type character. The specific function of this protein has not been determined, but its homologs in other species were described as differentiation antigens. PDPN can be used as a marker of lung injury. Alternatively spliced transcript variants encoding different isoforms have been identified.SpecificityThe mouse monoclonal antibody PcMab-47 recognizes a glycosylation-dependent epitope (aa 207-210) on human PODXL, a highly glycosylated transmembrane glycoprotein expressed above all in many types of cancer tissues, and on embryonic stem cells.Application detailsFlow cytometry: Recommended dilution: 1-5 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
LpMab-23
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
The tumour suppressor protein p53 is a key element of intracellular anticancer protection. It mediates cell cycle arrest or apoptosis in response to DNA damage or to starvation for pyrimidine nukleotides. It is up-regulated in response to these stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest, or proteins that trigger apoptosis, such as Bcl-2. The structure of p53 comprises N-terminal transactivation domain, central DNA-binding domain, oligomerisation domain, and C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization.SpecificityThe mouse monoclonal antibody LpMab-23 recognizes an extracellular epitope on human cancer type PDPN, a transmembrane glycoprotein, serving as a prognostic marker of oral carcinoma. This antibody recognizes an altered glycosylation pattern that occurs on oral cancer cells and it shows minimal reactivity with the surrounding non-cancerous tissue.Application detailsImmunohistochemistry (paraffin sections): Standard ABC technique (DAB+), pretreatment: high temperature antigen retrieval (microwave, pressure cooker) in 10 mM citrate buffer pH 6.0 or 1 mM EDTA-NaOH buffer pH 8.0, recommended dilution: 10 ?g/ml, incubation: 1 hour at RT; or overnight at 4°C, positive tissue: breast carcinoma with high level of wild-type p53. <br>Western blotting: recommended dilution: 1 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
FP3.2 [FPS392]
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
NKp80, also known as CLEC5C or KLRF1, is a type II transmembrane glycoprotein of the C lectin family, which is expressed in 80 kDa homodimers on NK cells, and subsets of CD8+ alpha/beta T cells, and gamma/delta T cells. It belongs to the activating coreceptors, which induce cytotoxicity, and production of pro-inflammatory cytokines. Its ligand AICL is expressed on myeloid cells.SpecificityThe mouse monoclonal antibody FP3.2 [FPS392] reacts with human p53 tumour suppressor intracellular protein phosphorylated at CKII site (Ser 392).Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
5D12
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Myeloperoxidase (MPO) is a heme enzyme that is localized in azurophilic (primary) granules of myeloid cells and its synthesis occurs at an early stage of differentiation. The mature myeloperoxidase is a tetramer composed of two light (12 kDa) and two heavy (60 kDa) chains. This enzyme uses hydrogen peroxide to oxidize numerous substrates, including serotonin, melatonin or chloride, to produce reactive free radicals that contribute to immune reactions of myeloid cells against pathogens. Myeloperoxidase functions not only in host defense by mediating efficient microbial killing but also can contribute to progressive tissue damage in chronic inflammatory states such as atherosclerosis or acute pancreatitis.SpecificityThe mouse monoclonal antibody 5D12 recognizes an extracellular epitope of human NKp80 (CLEC5C), a C-type lectin family member, expressed on NK cells and subsets of T cells.Application detailsFlow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MPO421-8B2
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
LOX1, a 31 kDa type II transmembrane protein, is a C-type lectin, functioning as a scavenger receptor for e.g. oxidized low density lipoprotein, apoptotic heat shock proteins, or CRP. It is expressed by macrophages, fibroblasts, platelets, endothelial cells, and smooth muscle cells, and its defects can lead to atherosclerosis. Its expression is enhanced under inflammatory conditions. Multiple splicing variants have been identified.SpecificityThe mouse monoclonal antibody MPO421-8B2 recognizes human myeloperoxidase, a heme protein present in intracellular granules of myeloblasts, neutrophils and monocytes. It is a marker of acute myelogenous leukemias and acute lymphoblastic leukemias.Application detailsFlow cytometry: Recommended dilution: 1-5 µg/ml
Antibody Isotype:
IgG2a kappa
Monosan Range:
MONOSAN
Clone:
15C4
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
LAR is a receptore-linked transmembrane protein tyrosine phosphatase expressed on mesenchymal stem cells, that reside e.g. in bone marrow, blood, placenta, adipose tissue, or skin, as well as it is expressed on some carcinoma cell lines, including HeLa, MCF-7, or HT29. During the process of externalization, LAR is intracellularly proteolytically processed into two non-covalently associated subunits. This protein is involved in intercellular and cell-matrix interactions and its extracellular part resembles that of cell adhesion molecules (CAMs). The extracellular part can be released from the surface, which may be used for regulation of LAR function.SpecificityThe mouse monoclonal antibody 15C4 recognizes an extracellular epitope of LOX1, a C-type lectin transmembrane protein.Application detailsFlow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
W7C6
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Immunoglobulin M (IgM) is produced as a 900 kDa pentamer, which is an efficient complement binder. This antibody type is produced initially in the immune response and it is the first immunoglobulin class to be synthesized by a fetus or newborn. IgM antibodies do not cross the placenta. IgM concentration in blood is 0.12 g/l and its biological survival (plasma T1/2) is 5 days.SpecificityThe mouse monoclonal antibody W7C6 recognizes an extracellular epitope of protein tyrosine phosphatase LAR, a marker of mesenchymal stem cells.Application detailsWestern blotting: Recommended dilution: 1 ?g/ml.<br>Flow cytometry: Recommended dilution: 1-4 ?g/ml, extracellular and intracellular staining.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
CH2
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75% of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.SpecificityThe antibody CH2 reacts with Fc fragment of human IgM.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
EM-07
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75% of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.SpecificityThe mouse monoclonal antibody EM-07 reacts with Fc part of human IgG heavy chain and with isolated Fc fragments.Application detailsFlow cytometry: Recommended dilution: 1-5 ?g/ml.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
4A11
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.SpecificityThe mouse monoclonal antibody 4A11 reacts with Fab fragment of human IgG. This antibody detects Fab fragments regardless light chain type.Application detailsFlow cytometry: Recommended dilution: 5 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
BE5
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Immunoglobulin D (IgD) is expressed on the surface of naive mature B cells, thus later than IgM, and is coexpressed with it then. Triggered by antigen binding, it signals through the CD79 complex to activate the B cells. Expression of IgD is lost after the isotype switch. Soluble IgD is present in very small amounts in the serum. IgD can bind to basophils and mast cells to activate them in an IgE-independent way to participate in respiratory immune defense.SpecificityThe antibody BE5 reacts with human IgE; it recognizes an epitope different from the ones recognized by 4G7 and 4H10 antibodies to IgE.Application detailsFlow cytometry: Recommended dilution: 2-4 ?g/ml.
Antibody Isotype:
IgG2a kappa
Monosan Range:
MONOSAN
Clone:
IA6-2
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda; both having a molecular weight of 22.5kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.SpecificityThe mouse monoclonal antibody AD3 reacts with alpha-chain of human IgA1 and IgA2.Application detailsFlow cytometry: Recommended dilution: 5 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-09
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
HER3 (ERBB3) is a transmembrane protein of the epidermal growth factor receptor family, although it does not have an active tyrosine kinase domain. It can bind its ligand, but for further signaling it needs heterodimerization with other receptor tyrosine kinases of EGFR family. Overexpression of HER3 has been observed in many carcinomas. Activity of HER3 can be modulated by one of its isoforms, that is secreted from the cell, as its lacks the transmembrane domain.SpecificityThe antibody MEM-09 reacts with both secreted and B cell-surface human immunoglobulin, specifically reacts with kappa light chains (22.5 kDa). Material immunoprecipitated from human serum with the antibody MEM-09 consists of IgG and traces of IgM.Application detailsFlow cytometry: Recommended dilution: 1-5 ?g/ml.
Antibody Isotype:
IgG2a kappa
Monosan Range:
MONOSAN
Clone:
H3Mab-17
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Granzyme A is a serine protease expressed in the cytoplasmic granules of T cells and NK cells. Vectorial secretion of perforin and granzymes is responsible for their granule-mediated cytotoxicity. Similarly to granzyme B, granzyme A acts to destroy the target cells by proteolysis of their particular components. In case of granzyme A the targets are e.g. APEX1 (it destroys its oxidative repair activity), and nucleosome assembly protein SET (it disrupts its nucleosome assembly activity and allows the SET complex to translocate into the nucleus to nick and degrade the DNA).SpecificityThe mouse monoclonal antibody H3Mab-17 recognizes an extracellular epitope on human HER3, a member of EGFR family of receptor tyrosine kinases, which is overexpressed in many cancers.Application detailsFlow cytometry: Recommended dilution: 5-10 ?g/ml, intracellular staining.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
CB9
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Galectin-9 is a glycan-binding protein, which is expressed in three main isoforms of 49 aa, 27 aa, and 15 aa. It can be detected on the cell surface, as well as intracellularly, or in a secreted form. On the cell surface, galectin-9 plays roles in contacts with other cells and with extracellular matrix. It is expressed on multiple cell types, but mainly on Treg cells, activated Th cells and some cancers. Its secreted form acts like a cytokine with immunomodulatory and immunosuppresive functions. Massive and inadequate production of galectin-9, associated with some viral infections or cancers, can counteract immune reactions to these illnesses. High levels of galectin-9 expression lead to poor prognosis of cancer patients.SpecificityThe mouse monoclonal CB9 recognizes granzyme A, a 28 kDa serine protease expressed intracellularly in activated Tc cells and NK cells.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml. Intracellular staining.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
9M1-3
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CLEC2 (C-type lectin-like receptor 2) functions as a platelet receptor for the lymphatic endothelial marker, PDPN, and mediates platelet activation. Besides platelets, it can be found on myeloid cells and NK cells. CLEC2 functions also as an attachment factor for HIV-1 and facilitates its capture by platelets. Platelet-aggregating snake venom protein rhodocytin also binds to CLEC2.SpecificityThe mouse monoclonal antibody 9M1-3 recognizes an epitope within C terminus of human galectin-9, a glycan-binding protein expressed mainly on activated Th cells and FoxP3+ Treg cells.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
AYP1
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD99 is a ubiquitous transmembrane type I sialoglycoprotein of a unique and poorly characterized protein family. CD99 is heavily O-glycosylated and was described as a T cell costimulator and strong activator of integrin-mediated actin cytoskeleton assembly, promoting cell adhesion and homotypic aggregation, immediate arrest on an inflamed vascular endothelium, and cell migration through it. Ligation of CD99 under some conditions can lead to apoptosis. Originally CD99 was described as a human thymus leukemia antigen, an Ewing´s sarcoma-specific membrane marker, and an adhesion molecule involved in spontaneous rosette formation of T cells with erythrocytes.SpecificityThe mouse monoclonal antibody AYP1 recognizes an epitope within the extracellular part of CLEC2, a transmembrane glycoprotein expressed on activated plateles and on platelet microparticles.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG2a kappa
Monosan Range:
MONOSAN
Clone:
3B2/TA8
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of the TNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular death domains, formation of the death-inducing signaling complex (DISC) and activation of caspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated at the DISC, in type II cells low concentration of caspase 8 activates pathway leading to the release of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokine responses.SpecificityThe mouse monoclonal antibody 3B2/TA8 recognizes CD99, an approximately 32 kDa sialoglycoprotein expressed on the surface of many cell types, with particularly strong expression on Ewing´s sarcoma and peripheral primitive neuroectodermal tumors. Within the hematopoietic system, CD99 is expressed on virtually all cell types except granulocytes.Application detailsFlow cytometry: Recommended dilution: 1-12 µg/ml. Excellent. <br>Immunohistochemistry (paraffin sections): Positive tissue: tonsil.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
LT95
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD94, also known as KLRD1 (killer cell lectin-like receptor D1), is a transmembrane glycoprotein of the C-type lectin family, which forms disulfide-linked heterodimers with NKG2A, B, C, E, H proteins, constituting functionally distinct receptors of NK cells and related cell types. CD94/NKG2A and CD94/NKG2B heterodimers serve as inhibitory, whereas CD94/NKG2C and CD94/NKG2E as activating receptors. The ligand for CD94/NKG2 complexes has been identified as HLA-E. Extent of CD94 expression on NK cell surface can be used to demonstrate their progress through the differentiation process.SpecificityThe antibody LT95 reacts with an extracellular epitope on CD95 (Fas/APO-1), a 46 kDa single chain type I glycoprotein of the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a variety of normal and neoplastic cells. It seems that the antibody LT95 does not induce Fas mediated apoptosis, although it cross-blocks anti-Fas DX2 antibody that recognizes a functional epitope of Fas molecule.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
HP-3D9
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD93 (also known as C1qR1) is a type I transmembrane glycoprotein containing extracellular N-terminal C-type lectin domain and five EGF-like domains, and an intracellular tail interacting with moesin, a protein known to play a role in linking transmembrane proteins to the cytoskeleton and in the remodelling of the cytoskeleton. CD93 was reported to serve as a receptor for complement component C1q, but this function has not been fully elucidated yet. CD93 is involved in intercellular adhesion and in the clearance of apoptotic cells.SpecificityThe mouse monoclonal antibody HP-3D9 recognizes an extracellular epitope of CD94, a 70 kDa type II transmembrane glycoprotein expressed on NK cells, NK-T cells, and subsets of CD8+ T cells and gamma/delta T cells.Application detailsFlow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
VIMD2
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD92 is a 70 kDa protein with ten transmembrane domains, intracellular N and C teminus, and two glycosylated larger extracellular loops. In the C-terminal domain, there is an ITIM-like sequence. This protein seems to be a choline transporter responsible for delivery of choline into the immune cells, to make it accessible for phospholipid synthesis, as well as a regulator of immune cell signaling. It is expressed mainly on human peripheral blood monocytes and neutrophils, and several myeloid and T-cell lines. It can also be found on mast cells (but not eosinophils), and weakly on peripheral blood lymphocytes, fibroblasts, epithelial cells, and endothelial cells.SpecificityThe mouse monoclonal antibody VIMD2 recognizes an extracellular epitope on CD93, an approximately 110-120 kDa glycoprotein expressed mainly on myeloid cells and endothelial cells.Application detailsFlow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
VIM15
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associated tetraspanin-enriched signaling microdomains within the plasma membrane, forming complexes with each other as well as with integrins, membrane-anchored growth factors and other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 is also considered as metastasis suppressor in solid tumors.SpecificityThe mouse monoclonal antibody VIM15 recognizes an extracellular epitope of CD92, a 70 kDa transmembrane glycoprotein expressed mainly on human peripheral blood monocytes and neutrophils, and several myeloid and T-cell lines.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml. <br>Western blotting: Recommended dilution: 2-4 ?g/ml, non-reducing conditions. <br>Immunohistochemistry (paraffin sections): Recommended dilution: 20 ?g/ml, positive tissue: prostate.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-61
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD88 / C5aR is a G protein-coupled seven membrane-spanning protein serving as a receptor for C5a component of the complement cascade, and is expressed mainly by monocytes, macrophages, neutrophils, eosinophils, and mast cells, but also e.g. by hepatocytes, glial cells, vascular endothelial cells, or cardiomyocytes. The binding of C5a to CD88 is associated with inflammatory response, including superoxide anion production, chemotaxis, and increased production of acute phase proteins. Expression of CD88 on synovial mast cells and their C5a-mediated degranulation plays a role in pathogenesis of rheumatoid arthritis.SpecificityThe antibody MEM-61 recognizes an epitope on second extracellular domain (EC2) of CD9 antigen, a 24 kDa transmembrane protein expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes.Application detailsFlow cytometry: Recommended dilution: 3-12 ?g/ml.
Antibody Isotype:
IgG2a kappa
Monosan Range:
MONOSAN
Clone:
S5/1
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD87, the urokinase plasminogen activator receptor (UPAR), is a GPI-anchored single chain glycoprotein of a 50-68 kDa, which is expressed on granulocytes, monocytes/macrophages, dendritic cells, endothelial cells, fibroblasts and keratinocytes. The urokinase plasminogen activator bound to CD87 converts plasminogen to plasmin, and being concentrated on the leading edge of migrating cells, it plays important role in cell adhesion and chemotaxis. CD87 binds to β1, β2, and β3 integrins, and can contribute to cancer cell invasion and metastasis. This antigen can also be used to study normal and abnormal granulopoiesis.SpecificityThe mouse monoclonal antibody S5/1 recognizes an extracellular epitope of CD88 protein, a 43 kDa receptor of C5a component of the complement cascade.Application detailsFlow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
VIM5
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.SpecificityThe mouse monoclonal antibody VIM5 recognizes CD87 (urokinase plasminogen activator receptor), a 36-68 kDa single-chain GPI-anchored extracellular glycoprotein expressed on granulocytes, monocytes/macrophages, dendritic cells, endothelial cells, fibroblasts and keratinocytes.Application detailsFlow cytometry: Recommended dilution: 5 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
BU63
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD85j, also known as ILT-2 (Ig-like transcript 2), LIR-1 (leukocyte Ig-like receptor 1), or LILRB1 (leukocyte Ig-like receptor B1), is a member of Ig superfamily transmembrane glycoproteins named CD85. The CD85j protein is expressed on several types of immune cells (plasma cells, B cells, monocytes, T and NK cell subsets) where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity.SpecificityThe mouse monoclonal antibody BU63 reacts with an extracellular epitope of CD86 (B7-2), a 70 kDa type I transmembrane glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG2b kappa
Monosan Range:
MONOSAN
Clone:
GHI/75
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD85g / ILT7 (immunoglobulin-like transcript 7) is a cell surface protein that is expressed on plasmacytoid dendritic cells (PDCs) and modulates the function of these cells in the immune response, such as the TLR-induced interferon production. It associates with gamma subunit of the high-affinity IgE receptor to form a receptor complex which transduces the signal through ITAM-associated downstream molecules. Expression of CD85g is downregulated by interleukin 3.SpecificityThe mouse monoclonal antibody GHI/75 recognizes an extracellular epitope of CD85j / ILT2, an 110-120 kDa membrane glycoprotein expressed strongly on plasma cells, moderately on circulating B cells, and weakly on monocytes. It is also expressed on T cell and NK cell subsets (variable, individual).Application detailsFlow cytometry: Recommended dilution: 2-5 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
17G10.2
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD84 is a highly glycosylated homophilic receptor of SLAM family. It is expressed on platelets and various types of leukocytes, especially following their activation. Ligation of CD84 leads to its phosphorylation on tyrosine residues within the cytoplasmic tail. These docking sites are recognized by downstream signaling molecules, such as phosphatase SHP-2 and adaptor protein SAP/SH2D1A. The function of CD84 has not been fully elucidated yet. Although predominantly activating receptor, its modulating activity was also demonstrated.SpecificityThe mouse monoclonal antibody 17G10.2 recognizes an extracellular epitope of CD85g / ILT7, a member of leukocyte immunoglobulin-like receptor family expressed on plasmacytoid dendritic cells, but not on myeloid dendritic cells and other peripheral blood leukocytes.Application detailsFlow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG2a kappa
Monosan Range:
MONOSAN
Clone:
CD84.1.21
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD83 is a 40-45 kDa heavily glycosylated type I cell surface glycoprotein of immunoglobulin family. It is expressed on the surface of mature dendritic cells, Langerhans cells in the skin, and interdigitating reticulum cells in the lymphoid tissues. Low expression of CD83 has been reported in activated T and B cells. Cytoplasmic expression of CD83 can be detected also in monocytes and macrophages. CD83 is involved in modulation of antigen presentation. Soluble CD83 has immunoregulatory functions, it is able to down-regulate dendritic cell maturation and stimulation of T cells. In the developing immune system, release of soluble CD83 from dendritic cells upon stimulation by gram-positive or gram-negative bacteria has anti-allergic effect. Herpes simplex virus, on the other hand, causes CD83 degradation in mature dendritic cells.SpecificityThe mouse monoclonal antibody CD84.1.21 recognizes an extracellular epitope of CD84, a single chain cell surface glycoprotein of 64-82 kDa, predominantly expressed B cells, monocytes, platelets and some T cells.Application detailsFlow cytometry:Recommended dilution: 1-4 µg/mL, Positive control: peripheral blood-derived dendritic cells. <br>Immunohistochemistry (frozen sections): acetone fixation.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
HB15e
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD82 (KAI1), a member of the tetraspanin family, forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules. These complexes influence adhesion, morphology, activation, proliferation and differentiation of B, T and other cells. CD82 regulates cytoskeleton rearrangement and may participate in the turnover of the tetraspanin complex members. Besides in the plasma membrane, CD82 is localized also in endosome/lysosome compartments. Tumour-suppressive roles of CD82 have been demonstrated.SpecificityThe mouse monoclonal antibody HB15e recognizes an extracellular epitope of CD83, a 40-45 kDa type I glycoprotein expressed on mature dendritic cells.Application detailsFlow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
C33
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD81 (TAPA-1), a member of the tetraspanin family, is expressed on virtually all nucleated cells, but above all on germinal center B cells. CD81 forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules, and influences adhesion, morphology, activation, proliferation and differentiation of B, T and other cells, e.g. in muscles CD81 promotes cell fusion and myotube maintenance. CD81 has been also identified as a receptor for the hepatitis C virus.SpecificityThe mouse monoclonal antibody C33 recognizes an extracellular/luminal epitope of CD82, a widely expressed cell surface protein of the tetraspanin family. CD82 is also found in endosome/lysosome compartments.Application detailsFlow cytometry: recommended dilution: 1 ?g/ml.<br>Western blotting: recommended dilution: 1-2 ?g/ml; positive control: Jurkat cells, non-reducing conditions.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
M38
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
The CD8 T cell coreceptor (monomer approx. 32-34 kDa) is expressed as alpha/beta heterodimer on majority of MHC I-restricted conventional T cells and thymocytes and as alpha/alpha homodimer on subsets of memory T cells, intraepithelial lymphocytes, NK cells and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha-beta but not alpha-alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.SpecificityThe antibody M38 reacts with an extracellular epitope of CD81, a 25 kDa member of the tetraspanin family, expressed on majority of cells.Application detailsFlow cytometry: Recommended dilution: 2 ?g/ml. <br>Immunoprecipitation: Excellent for immunoisolation of CD8 positive T cells.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-87
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD79b (Ig beta, B29) forms disulfide-linked heterodimer with CD79a (Ig alpha, MB1). They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR). CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with lambda5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling.SpecificityThe antibody MEM-87 recognizes an extracellular epitope of CD8, a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. CD8 is a disulfide-linked dimer and exists as a CD8 alpha/alpha homodimer or CD8 alpha/beta heterodimer (each monomer approx. 32-34 kDa).Application detailsFlow cytometry: Recommended dilution: 1-5 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
CB3-1
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD79a (Ig alpha, MB1) forms disulfide-linked heterodimer with CD79b (Ig beta). They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR). CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with lambda5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. At the plasma cell stage, CD79a is present as an intracellular component. CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling.SpecificityThe mouse monoclonal antibody CB3-1 recognizes an extracellular epitope of CD79b (CD79 beta, Ig beta), an approximately 38 kDa component of B cell receptor (BCR) complex.Application detailsFlow cytometry: Recommended dilution: 5 ?g/ml. Intracellular staining.<br>Immunohistochemistry (paraffin sections): Recommended dilution: 10 ?g/ml.<br>Immunohistochemistry (frozen sections): Recommended dilution: 10 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
HM57
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD75 is a lactosamine structure, which is present mainly on the surface of germinal center B cells. With lower level it is present on other mature B cells, and it is downregulated during differentiation to plasma cells. It is a marker which differentiates between malignant B cell types.SpecificityThe antibody HM57 interacts with intracellular domain of CD79a (Ig alpha), a 40-45 kDa subunit of B cell antigen-specific receptor (BCR) and its early developmental forms.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgM kappa
Monosan Range:
MONOSAN
Clone:
LN1
Concentration:
1 mg/ml
Format:
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Storage buffer:
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
CD74 (the MHC II-associated invariant chain, Ii) is a type II transmembrane protein expressed in antigen-presenting cells, that serves as MHC II chaperone which promotes MHC II trafficking from the ER to endocytic compartments, prevents peptide binding in the ER and contributes to peptide editing in the MHC II compartment; it is also an accessory signaling molecule implicated e.g. in malignant B cell proliferation. Stimulation of cell surface CD74 leads to NFkappaB activation, which enables entry of the stimulated cell into the S phase. CD74 binds pro-inflammatory cytokine MIF with high affinity and interacts with CD44. Binding of Vpu, an HIV1 protein, to CD74 modulates MHC II presentation.SpecificityThe mouse monoclonal antibody LN1 recognizes CD75, a lactosamine structure present mainly on the surface of B cell types.Application detailsFlow cytometry: Recommended dilution: 2-5 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
LN2
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD73 (ecto-5´-nucleotidase) is a 70 kDa glycoprotein anchored to the extracellular leaflet of the plasma membrane by GPI. This ecto-enzyme catalyzes dephosphorylation of AMP to adenosine. CD73 is expressed in various types of cells, such as epithelial, muscle, and endothelial cells, neutrophils, lymphocytes and fibroblasts. Inflammatory mediators support CD73 expression and its enzymatic activity, leading to the release of adenosine, which modulates inflammation through adenosine receptors. CD73 is expressed in a variety of lymphomas and leukemias, including ALL and CLL, whereas immunodeficient patients usually express low levels of this protein.SpecificityThe antibody LN2 recognizes an extracellular epitope of CD74 (the MHC II-associated invariant chain, Ii), a type II transmembrane protein expressed in antigen-presenting cells, as well as e.g. on activated neoplastic cells in T cell lymphomas, in lymph node germinal center, mantle zone B cells, histiocytes, interdigitating reticulum cells, Langerhans cells, thymic dendritic cells and peripheral blood B lymphocytes.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
AD2
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD72 is a transmembrane glycoprotein expressed as a homodimer especially in B cells, but also in other antigen presenting cells such as dendritic cells and macrophages. Through one of its immunoreceptor tyrosine-based inhibitory motives (ITIMs), CD72 interacts with tyrosine phosphatase SHP-1, thereby suppressing B cell responsiveness. Binding of CD72 with its ligand CD100 (Sema4D) prevents BCR association and phosphorylation of CD72 and results in dissociation of SHP-1 from CD72, thus enables B cell activation.SpecificityThe mouse monoclonal antibody AD2 recognizes CD73, a 70 kDa GPI-anchored 5´-nucleotidase expressed predominantly on the surface of T and B cell subsets, follicular dendritic cells and endothelial cells.Application detailsFlow cytometry: Recommended dilution: 1 ?g/ml.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
3F3
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH. CD71 is also involved in uptake of non-transferrin bound iron.SpecificityThe mouse monoclonal antibody 3F3 recognizes an extracellular epitope of CD72, a 39-43 kDa type II membrane glycoprotein (C-type lectin family). CD72 is a pan-B cell marker expressed throughout the B lymphocytes diferentiation with the exception of plasma cells; it is also present on follicular dendritic cells.Application detailsFlow cytometry: Recommended dilution: 2 ?g/ml. <br>Western blotting: Non-reducing conditions.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-189
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
CD70, also known as TNFSF7 or CD27L, is a 50 kDa type II transmembrane glycoprotein of the TNF superfamily. It is expressed mainly on activated lymphocytes, including NK cells, and forms trimeric structure. CD70 plays a role in T-cell activation, proliferation and differentiation, in enhancing the generation of cytolytic T cells, and in long-term maintenance of T cell memory. It is also involved in B cell differentiation induced by activated plasmacytoid dendritic cells, which also express CD70.SpecificityThe antibody MEM-189 reacts with an extracellular epitope on CD71 antigen (transferrin receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and lymphocytes, macrophages and erythroid precursors; it is lost on resting blood leukocytes. The antibody MEM-189 does not block binding of transferrin to the receptor.Application detailsFlow cytometry: Recommended dilution: 1-5 ?g/ml.
Antibody Isotype:
IgG3 kappa
Monosan Range:
MONOSAN
Clone:
Ki-24
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD7, also known as gp40, is a member of the immunoglobulin superfamily found on T cells, NK cells, thymocytes, hematopoietic progenitors, and monocytes (weakly). CD7 is also expressed on acute lymphocytic leukemia (ALL). CD7 crosslinking induces a calcium flux in T lymphocytes, presumably as a result of cytoplasmic domain association with PI3-kinase. CD7 co-stimulation can induce cytokine secretion and modulate cellular adhesion. A ligand of CD7, epithelial cell secreted protein K12, is produced in thymus to regulate thymocyte signaling and cytokine release. In lung microvascular endothelial cells CD7 serves as an IgM Fc receptor. Expression of CD7 is an important marker used in leukemia diagnostics.SpecificityThe mouse monoclonal antibody Ki-24 recognizes an extracellular epitope of CD70, an approximately 50 kDa type II transmembrane glycoprotein expressed on activated lymphocytes and some B cell leukemias.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
124-1D1
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD69 (C-type lectin domain family 2 C, CLEC2C, also known as AIM) is one of the earliest inducible cell surface molecules acquired during leukocyte activation. This glycoprotein serves as a lectin-type receptor in lymphocytes, NK cells and platelets; it is involved in lymphocyte proliferation. CD69 expression is counteracted on T cells in the AIDS stage of HIV infection, and may be also predictive for clinical response to chemoimmunotherapy.SpecificityThe mouse monoclonal antibody 124-1D1 recognizes an extracellular epitope of CD7, a 40 kD type I transmembrane glycoprotein expressed on peripheral blood T lymphocytes, NK-cells, hematopoietic progenitors, monocytes (weakly) and also on acute lymphocytic leukemia.Application detailsFlow cytometry: recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
FN50
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD68 (also known as LAMP4 or SCARD1) is a 110 kDa type I transmembrane glycoprotein of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and the scavenger receptor family. Although CD68 primarily localizes to lysosomes and endosomes, its fraction circulates to the cell surface. By the heavily glycosylated extracellular domain CD68 binds to tissue- and organ-specific lectins or selectins. It is expressed mainly in cytoplasmic granules of monocytes/macrophages, granulocytes, and dendritic cells, but also e.g. in a proportion of epithelial tumours (diagnosis of poorly differentiated neoplasms).SpecificityThe mouse monoclonal antibody FN50 recognizes an extracellular epitope of CD69, an lymphocyte early activation marker.Application detailsFlow cytometry: Recommended dilution: 2-6 ?g/ml. Extracellular and intracellular staining.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
Y1/82A
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
The CD66e (CEA; 180-200 kDa) is a member of carcinoembryonic antigens, immunoglobulin supergene family and consists of a single N domain (structural homology to the immunoglobulin variable) and six immunoglobulin constant-like A (A1, A2, A3) and B domains (B1, B2, B3). Human CD66e is heavily glycosylated GPI anchored protein capable of both homophilic and heterophilic adhesion. Disease relevance: The CD66e may play a role in the process of metastasis of cancer cells. CD66e is found in serum and it is clinically used as a tumor marker for early detection of disease due to its expression in adenocarcinomas - potential target of tumor imaging and drug targeting.SpecificityThe mouse monoclonal antibody Y1/82A recognizes CD68 (LAMP4), a 110 kDa glycoprotein expressed mainly in cytoplasmic granules of monocytes/macrophages, granulocytes, and dendritic cells.Application detailsImmunohistochemistry (paraffin sections): Staining technique: standard ABC technique (DAB+), recommended dilution: 10 ?g/ml (1:100), positive tissue: adenocarcinoma of colon, pretreatment: 0,1% pepsin in 0,1 M HCl for 30 min at room temperature.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
CB30
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD66c is a GPI-anchored glycoprotein capable of homophilic adhesion and heterophilic binding to CD66a-e, CD62E, and galectins. It is expressed on granulocytes and epithelial cells, and has potential applications in the detection of sites of infection and inflammation.SpecificityThe mouse monoclonal antibody CB30 recognizes CD66e (CEA; 180-200 kDa), an extracellular cell surface-bound carcinoembryonic antigen mainly expressed on epithelial cells.Application detailsFlow cytometry: Recommended dilution: 3-12 µg/ml
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
B6.2
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD65 is a fucosylated carbohydrate antigen (ceramide-dodecasaccharide, type II fucoganglioside), which serves as a ligand for CD62E (E-selectin). Its structure is Gal beta1-4 GlcNAc beta1-3 Gal beta1-4 GlcNAc (3-1 Fuc alpha) beta1-3 ceramide. Unlike CD65s, the CD65 antigen does not contain terminal sialic acid, the rest of their structure is identical. CD65 is expressed on granulocytes and monocytes and participates in cell adhesion. It has been reported as important for extravascular infiltration of acute monocytic leukemia cells.SpecificityThe mouse monoclonal antibody B6.2 recognizes a conformationally dependent epitope of native CD66c, a GPI-anchored extracellular glycoprotein expressed on granulocytes and epithelial cells.Application detailsFlow cytometry: Recommended dilution: 1-5 ?g/ml.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
VIM8
Concentration:
1 mg/ml
Format:
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Storage buffer:
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
CD64 (FcgammaRI) is a cell surface receptor for Fc region of IgG. It is composed of specific ligand binding alpha subunit and promiscuous gamma subunit, which is indispensable for tyrosine-based signaling. However, even the alpha subunit can transduce signals leading to cellular effector functions. The isoform FcgammaRIa1 binds human IgG with high affinity, has limited myeloid cell distribution, and a relatively large intracellular domain. Products of related genes include FcgammaRIb and FcgammaRIc isoforms, but these specify low affinity IgG receptors if functionally expressed at all. Besides a role in antigen clearance, FcgammaRI (a1) can potently enhance MHC class I and II antigen presentation in vitro and in vivo.SpecificityThe mouse monoclonal antibody VIM8 recognizes human CD65, an asialo-fucoganglioside expressed on the surface of peripheral blood granulocytes (highly) and monocytes (weakly).Application detailsFlow cytometry: Recommended dilution: 1-5 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
10.1
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD62P (P-selectin) is an adhesion glycoprotein that is expressed on platelets and endothelial cells upon their activation. Interaction between CD62P and its mucin-like ligand PSGL-1 (P-selectin glycoprotein ligand-1) expressed on the microvilli of most leukocytes supports leukocyte rolling along postkapillary venules at the earliest time of inflammation. Both CD62P and PSGL-1 are extended glycoproteins that form homodimers. CD62P dimerization is probably mediated through interactions of the transmembrane domains and stabilizes leukocyte tethering and rolling, probably by increasing rebinding within a bond cluster.SpecificityThe mouse monoclonal antibody 10.1 recognizes an extracellular epitope on CD64/FcgammaRI, a 72 kDa single chain type I glycoprotein, that is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes.Application detailsFlow cytometry: Recommended dilution: 1-5 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
HI62P
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1).SpecificityThe mouse monoclonal antibody HI62P recognizes an extracellular epitope of CD62P (P-selectin), a 140 kD single chain type I transmembrane glycoprotein present in secretory alpha-granules in platelets, in Weibel-Palade bodies in endothelial cells and in megakaryocytes; it is relocated to the plasma membrane upon activation.Application detailsFlow cytometry: Recommended dilution: 1-5 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
LT-TD180
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD61 (beta3 integrin) is a transmembrane glycoprotein, which associates with CD41 or CD51 molecules to form heterodimeric adhesion receptores. CD41/CD61 complex is one of the earliest markers of the megakaryocytic lineage. It binds to fibronectin, fibrinogen and von Willebrand factor, and is involved in platelet aggregation. CD51/CD61 complex has similar binding properties and is involved in modulating migration and survival of angiogenic endothelial cells.SpecificityThe antibody LT-TD180 reacts with an extracellular epitope of CD62L (L-selectin), a 74-95 kDa single chain type I glycoprotein expressed on most peripheral blood B lymphocytes, T lymphocytes, monocytes and granulocytes; it is also present on a subset of NK cells and certain hematopoietic malignant cells.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
VIPL2
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD58 (LFA-3) is an immunoglobulin family adhession molecule expressed by both hematopoietic and non-hematopoietic cells (often on antigen presenting cells) and serving as ligand of CD2. This interaction is important for T cell-mediated immunity. CD58 is expressed in transmembrane form and in GPI-anchored form; the later is constitutively associated with protein kinases whereas the transmembrane form activates kinase activity upon triggering. CD58 is a powerful tool for detection of minimal residual disease in acute lymphocytic leukemia, and for evaluation of liver damage related with hepatitis B.SpecificityThe mouse monoclonal antibody VIPL2 recognizes an extracellular epitope of CD61, a 90-110 kDa transmembrane glycoprotein of integrin family, expressed on platelets, megacaryocytes, osteoclasts, endothelial cells and other cell types, including leucocytes and smooth muscle cells.Application detailsFlow cytometry: Recommended dilution: 1-4 µg/ml. Cold-induced transient opalescent turbidity may appear during the storage at 2 - 8°C. Bring the antibody to room temperature until the turbidity disappears, and mix well before the use.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-63
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 8.8, 15 mM sodium azide
CD57, also known as HNK1 or Leu7, is a sulphated trisaccharide (3-O-sulfoglucuronic acid beta1-3 Gal beta1-4 GlcNAc) attached to several glycoproteins, including CD56, myelin glycoprotein PO, and neural cell adhesion molecule L1, as well as on glycolipids and chondroitin sulphate proteoglycans in the nervous system. It serves as a NK cell marker and it is expressed on well differentiated prostate cancers and uveal and cutaneous melanoma. CD57+ T cells are implicated as suppressors of T-cell responses.SpecificityThe antibody MEM-63 reacts with CD58 (LFA-3), a 40-70 kDa extracellular membrane glycoprotein distributed over many tissues, leukocytes, erythrocytes, endothelial cells, epithelial cells and fibroblasts.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml. <br>Immunohistochemistry: Recommended dilution: 5-10 ?g/ml.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
TB01
Concentration:
1 mg/ml
Format:
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Storage buffer:
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of immunoglobulin family serving as adhesive molecule which is ubiquitously expressed in nervous system, usually as 120 kDa, 140 kDa or 180 kDa isoform, and it is also found on T cells and NK cells. Polysialic modification results in reduction of CD56-mediated cell adhesion and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity. CD56 is a widely used neuroendocrine marker with a high sensitivity for neuroendocrine tumours and ovarian granulosa cell tumours.SpecificityThe mouse monoclonal antibody TB01 recognizes CD57, a carbohydrate extracellular antigen present mainly on NK cells, NK T cells, and in neural tissue.Application detailsWestern blotting: Non-reducing conditions. <br>Flow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
LT56
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD52 (CAMPATH-1, HE5) is a highly glycosylated GPI-anchored 21-28 kDa glycopeptide which is present at high levels on lymphocytes, macrophages, epithelial cells of male reproductive tract and mature sperm. Its 12-amino acid beckbone carries a complex N-linked carbohydrate moiety, which differs between sperm and leukocyte CD52, as well as the GPI anchor does. CD52 can be acquired by sperm cells from seminal plasma, where it is released by epithelial cells. Although CD52 is not an essential T-cell costimulator, its triggering results in activation of normal human T cells. CD52 is a very good target for antibody/complement-mediated cell lysis.SpecificityThe mouse monoclonal antibody LT56 recognizes an extracellular epitope of CD56 (NCAM), a transmembrane glycoprotein expressed ubiquitously in the nervous system and found also on T cells and NK cells.Application detailsFlow cytometry: Recommended dilution: 2 ?g/ml.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
HI186
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD51/CD61 (integrin alpha5beta3), also known as osteoclast functional antigen, serves as a vitronectin receptor, and binds also to fibronectin, fibrinogen, thrombospondin, osteopontin, collagen, and von Willebrand factor. Expression of this antigen increases with melanoma progression. In healthy individuals CD51/CD61 is expressed mainly on osteoclasts, placenta, and endothelial cells, at lower levels on platelets and macrophages.SpecificityThe antibody HI186 reacts with CD52 (CAMPATH-1), a 21-28 kDa extracellular glycoprotein containing a large N-linked carbohydrate moiety; mature CD52 molecule is actually much smaller (approx. 8-9 kDa). CD52 is expressed at high levels on lymphocytes, monocytes/macrophages and in male reproductive tract.Application detailsFlow cytometry: Recommended dilution: 2-6 µg/ml
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
23C6
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD5 antigen (T1; 67 kDa) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains. The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. Preliminary evidence shows protein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells it seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies. Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative disorders (B-CLL, Hairy cell leukemia, etc.). The CD5+ popuation is expanded in some autoimmune disorders (rheumatoid arthritis, etc.). Herpes virus infections induce loss of CD5 expression in the expanded CD8+ human T cells.SpecificityThe mouse monoclonal antibody 23C6 recognizes an extracellular epitope on CD51/CD61 complex, that is expressed mainly on human osteoclasts, but also e.g. on placenta, or melanoma cell lines. The epitope is native and sensitive to fixation. In chicken this antibody can be used to selectively identify the thrombocytes.Application detailsELISA: The antibody MEM-32 can be used in the Sandwich ELISA as the capture antibody in pair with the detection antibody CRIS1. <br>Immunohistochemistry (paraffin sections): Recommended dilution: 20 ?g/ml; positive tissue: spleen. <br>Flow cytometry: Recommended dilution: 2 ?g/ml. <br>Western blotting: Laurylmaltoside lysing buffer; non-reducing conditions; recommended dilution: 1-2 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-32
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD49e (VLA5 alpha) is a type I transmembrane glycoprotein of the integrin alpha subclass (intergrin 5 alpha), expressed on thymocytes, early and activated B cells, monocytes, NK cells, dendritic cells, osteoblast and endothelial cells. It binds to RGD sequence in fibronectin and to neural adhesion molecule L1. CD49e interactions are important for maintaining the integrity of the endothelial monolayer, as well as it is involved in monocyte migration, T cell costimulation, regulation of cell survival, and other.SpecificityThe antibody MEM-32 reacts with an extracellular epitope of CD5, a 67kDa single-chain transmembrane glycoprotein expressed on mature T-lymphocytes, most of thymocytes and B-lymphocytes subset (B-1a lymphocytes).Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
SAM1
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD49d / integrin alpha 4, unlike other alpha integrins, neither contains an I-domain, nor undergoes disulfide-linked cleavage. It associates with beta 7 chain to form alpha 4 / beta 7 integrin, and with beta 1 chain (CD29) to form VLA-4 integrin. These complexes are important for lymphocyte migration from circulation into tissue (binding VCAM-1) and homing of T cell subsets to Peyer´s patches (binding MadCAM-1), but VLA-4 is also target for invasive bacteria which contain invasin. CD49d is essential for differentiation and migration of hematopoietic stem cells by their adhesion to bone marrow stromal cells, and provides a costimulatory signal to TCR-CD3 complex by inducing phosphorylation of some focal adhesion proteins.SpecificityThe mouse monoclonal antibody SAM1 recognizes an extracellular epitope of CD49e (integrin 5 alpha), a transmembrane glycoprotein expressed on thymocytes, early and activated B cells, monocytes, NK cells, dendritic cells, osteoblast and endothelial cells.Application detailsImmunohistochemistry: Recommended dilution: 5-10 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
9F10
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD49c / Integrin alpha 3 is a type I transmembrane glycoprotein proteolytically cleaved into two disulfide linked chains. It noncovalently associates with CD29 (integrin beta 1) to form the VLA-3 complex, an adhesion receptor for extracellular matrix components (fibronectin, laminin 1, laminin 5, entactin, and collagen). It is expressed on adherent cells, mainly on fibroblasts, epithelial cells and endothelial cells.SpecificityThe mouse monoclonal antibody 9F10 recognizes an extracellular epitope of CD49d (alpha 4 integrin), a 145-180 kDa type I transmembrane glycoprotein expressed on B and T cells, monocytes, eosinophils, basophils, NK cells, and dendritic cells, but not platelets.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
ASC-1
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD49b the integrin alpha 2 chain, associates with CD29 (integrin beta 1 chain) to form VLA-2 integrin complex, which plays a critical role in the processes of lymphocyte adhesion and activation. VLA-2 serves as a receptor for collagen, laminin, and fibronectin and also regulates the extracellular matrix synthesis and organization. CD49b has been used to identify NK cells, and coexpressed with CD223 (LAG-3) it identifies CD4+ T regulatory type 1 cells (Tr1).SpecificityThe mouse monoclonal antibody ASC-1 recognizes an extracellular epitope of CD49c (integrin alpha 3), a transmembrane glycoprotein composed of disulfide linked 125 kDa and 30 kDa chains, and expressed on adherent cell lines and to a lesser extent on T and B cells and monocytes.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml. <br>Immunohistochemistry: Recommended dilution: 5-10 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
AK7
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD49a is the alpha 1 chain of VLA integrin complex (together with CD29, serving as the beta 1 chain), and is expressed on activated T cells, monocytes, NK cells, cultured neuronal cells, melanoma cells, mesenchymal cells (including smooth muscle cells), fibroblasts, hepatocytes, and microvascular endothelium. It binds to collagen IV and laminin 1. It is important for leukocyte migration into tissues. It is upregulated in inflammatory tissues, such as inflammed intestine.SpecificityThe mouse monoclonal antibody AK7 recognizes an extracellular epitope of CD49b, a 160-165 kDa alpha subunit of VLA-2 integrin complex expressed on platelets, megakaryocytes, activated T and B cells, monocytes, epithelial cells, endothelial cells and fibroblasts.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
TS2/7
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD47 (integrin-associated protein, IAP) is an ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Binding secreted thrombospondin-1, CD47 counteracts graft vascularization. CD47 acts also as a ligand for CD172a (signal regulatory protein alpha, SIRP alpha), an immune inhibitory receptor on macrophages; this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion and T cell activation. CD47 is also involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.SpecificityThe mouse monoclonal antibody TS2/7 recognizes an extracellular epitope of CD49a, the alpha 1 chain of VLA integrin complex, that serves as an adhesion receptor for collagen IV and laminin 1.Application detailsFlow cytometry: Recommended dilution: 2 ?g/ml. <br>Western blotting: Non-reducing conditions.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
MEM-122
Concentration:
1 mg/ml
Format:
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Storage buffer:
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
CD45R0 is the shortest isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45R0 is expressed e.g. on macrophages, CD8+ T cells, activated T cells and myeloma cells.SpecificityThe antibody MEM-122 reacts with an extracellular epitope of CD47 (Integrin Associated Protein), a 50-55 kDa membrane adhesion molecule (thrombospondin receptor; immunoglobulin supergene family) expressed on leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines.Application detailsImmunohistochemistry (paraffin sections): This product does not require protein digestion pretreatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. Positive tissue: tonsil. <br>Immunohistochemistry (frozen sections): Positive tissue: tonsil. <br>Flow cytometry: Recommended dilution: 10 ?g/ml.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
UCHL1
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.SpecificityThe antibody UCHL1 recognizes an extracellular epitope of CD45R0, a 180 kDa low molecular weight isoform of the leukocyte common antigen (LCA). The antigen is expressed on a subset of memory/activated T cells and on cortical thymocytes.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
2D1
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD42a, also known as glycoprotein 9 (GPIX), composes together with GPIb alpha, GPIb beta and GPV the GPIb-IX-V receptor complex critical in the process of platelet-rich thrombus formation by tethering the platelet to a thrombogenic surface. CD42b binds to von Willebrand factor (VWF) exposed at a site of vascular injury, as well as to thrombin, coagulation factors XI and XII, high molecular wight kininogen, TSP-1, integrin Mac-1 and P-selectin. Defects in the gene encoding CD42a are a cause of Bernard-Soulier syndrome, also known as giant platelet disease. These patients have unusually large platelets and have a clinical bleeding tendency.SpecificityThe mouse monoclonal antibody 2D1 reacts with an extracellular epitope of all alternative forms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except from erythrocytes and platelets.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
GR-P
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD41 (platelet glycoprotein IIb) is composed of two subunits (120 kDa a, alpha and 23 kDa b, beta) that interact with CD61 in the presence of calcium to form a functional adhesive protein receptor. Upon blood vessel damage, this receptor binds to a variety of proteins including von Willebrand factor, fibrinogen, fibronectin and vitronectin. CD41 is mainly expressed on megakaryocyte-platelet lineage, but generally belongs to the antigens that are expressed during early stages of hematopoietic differentiation.SpecificityThe mouse monoclonal antibody GR-P (also known as GRP-P) recognizes an extracellular epitope of CD42a (glycoprotein 9), a 22 kDa transmembrane protein constitutively expressed on megakaryocytes and platelets.Application detailsFlow cytometry: Recommended dilution: 2 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-06
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).SpecificityThe antibody MEM-06 reacts with an extracellular epitope of CD41 (GPIIb), a transmembrane glycoprotein (integrin family) composed of two chains GPIIb alpha (heavy chain; 120 kDa) and GPIIb beta (light chain; 23 kDa). CD41 is mainly expressed on platelets and megakaryocytes.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml; positive control: peripheral blood lymphocytes.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
MEM-16
Concentration:
1 mg/ml
Format:
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Storage buffer:
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
CD39, also known as ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1), is a cell surface enzyme (with intracellular N- and C-terminus) which hydrolyzes extracellular ATP and ADP to AMP. Inhibition of its enzymatic activity may confer anticancer benefits. The formation of oligomers in the plasma membrane is essential for enzyme activity. It is expressed on Treg cells, and in other cell types, such as mantle zone B cells, activated T cells, NK cells, macrophages, dendritic cells, neurons, endothelial cells and platelets. Hydrolysis of ATP and ADP inhibits inflammatory and thrombotic responses. In the nervous system, it regulates purinergic neurotransmission.SpecificityThe antibody MEM-16 recognizes an extracellular epitope in EF loop of D1 domain of CD4 antigen, a 55 kDa transmebrane glycoprotein expressed on a subset of T lymphocytes (“helper“ T-cells) and also on monocytes, tissue macrophages and granulocytes.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG2b kappa
Monosan Range:
MONOSAN
Clone:
TU66
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD38 (NAD+ glycohydrolase) is a type II transmembrane glycoprotein able to induce activation, proliferation and differentiation of mature lymphocytes and mediate apoptosis of myeloid and lymphoid progenitor cells. Another role of CD38 is provided by enzymatic activity of its extracellular part. CD38 acts as NAD+ glycohydrolase converting NAD+ into ADP-ribose, as ADP-ribosyl cyclase producing cADPR and as cADPR hydrolase, thus affecting levels of calcium-mobilizing metabolites. ADPR produced by CD38 serves as an important second messenger of neutrophil and dendritic cell migration.SpecificityThe mouse monoclonal antibody TU66, also known as Tü66, recognizes an extracellular epitope of CD39, a 78 kDa cell surface enzyme expressed by regulatory T cells, mantle zone B cells, activated T cells, NK cells, macrophages, dendritic cells, neurons, endothelial cells and platelets.Application detailsImmunohistochemistry (paraffin sections): Recommended dilution: 10 ?g/ml. <br>Western blotting: Recommended dilution: 2 ?g/ml; positive control: RAJI human cell line, non-reducing conditions. <br>Flow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
HIT2
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD37 is a 40-64 kDa tetraspanin family glycoprotein, which forms complexes in the B cell membrane with MHC class II, CD53, CD81, and CD82. It is expressed highly on mature B cells and neoplastic B cells, but it is lost on plasma cells, as well as on pro-B cells. Lower expression was detected on monocytes, macrophages, and dendritic cells.SpecificityThe mouse monoclonal antibody HIT2 reacts with an extracellular epitope of CD38, a 45 kDa type II transmembrane glycoprotein strongly expressed mainly on plasma cells and activated T and B lymphocytes; it is an antigenic marker of lymphoid cells. Its binding is blocked by daratumumab.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
MB-1
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD369 (dectin-1, beta-glucan receptor) is a 33 kDa type II transmembrane glycoprotein of lectin family, and serves as a part of innate immunity system by binding to beta-glucan polymers, which are typical for yeast and mycobacterial cell walls. CD369 is expressed predominantly on dendritic cells, but it can be detected also on monocytes, macrophages, mast cells, eosinophils, B cells, endothelial cells, and sometimes also on some T cell subsets.SpecificityThe mouse monoclonal antibody MB-1 recognizes an extracellular epitope of CD37, a tetraspanin family transmembrane glycoprotein.Application detailsFlow cytometry: Recommended dilution: 2-5 ?g/ml.
Antibody Isotype:
IgG2a kappa
Monosan Range:
MONOSAN
Clone:
15E2
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD367 is an approximately 20-28 kDa C-type lectin with immunoreceptor tyrosine-based inhibitory motif (ITIM) in its cytoplasmic part. CD367 binds in calcium-dependent manner to mannose, fucose, and weakly also to N-acetylglucosamine. It is expressed on dendritic cells, macrophages, monocytes, B cells, and neutrophils. In rheumatoid arthitis patients CD367 is expressed also on CD4+ T cells. After ligand-mediated triggering, it is internalized by clathrin-dependent endocytosis and contributes to the antigen presentation to CD8+ T cells. It may also be involved in modulation of the antigen presenting cell response.SpecificityThe mouse monoclonal antibody 15E2 recognizes an extracellular epitope LWEDGSTFSSN of human CD369 (Dectin-1), a 33 kDa transmembrane glycoprotein expressed predominantly on dendritic cells.Application detailsFlow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
9E8
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD361, also known as EVI2B (ecotropic viral integration site 2B) or EVDB, is a poorly characterized type I transmembrane protein, expressed from one of three genes embedded in intron 27b of the neurofibromatosis type 1 (NF1) gene. The DNA strand that is transcribed to produce CD361 is the complementary one to the strand encoding NF1. Murine homolog to human CD361 is associated with ecotropic viral insertions, which have been implicated in the expression of murine myeloid leukemias. CD361 has been also reported to be involved in melanocyte and keratinocyte differentiation. However, it is expressed mainly in peripheral blood and bone marrow.SpecificityThe mouse monoclonal antibody 9E8 recognizes an extracellular epitope of human CD367, a type II transmembrane protein of C-lectin family, expressed mainly on antigen presenting cells.Application detailsFlow cytometry: Recommended dilution: 1-4 µg/ml, Positive control, Positive control: Raji, Daudi, HL-60 cells, peripheral blood lymphcocytes (strongly positive on CD19+ cells), negative control: Jurkat, U-937 cells.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
MEM-216
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD36 (fatty acid translocase, FAT) is an 88 kDa ditopic glycosylated protein that belongs to the class B family of scavenger receptors. CD36 is expressed by most resting marginal zone B cells but not by follicular and B1 B cells, and it is rapidly induced on follicular B cells in vitro upon TLR and CD40 stimulation. CD36 does not affect the development of B cells, but modulates both primary and secondary antibody response. Similarly to glucose transporter GLUT4, CD36 is translocated from intracellular pools to the plasma membrane following cell stimulation by insulin. In mouse, CD36 is responsible for gustatory perception of long-chain fatty acids.SpecificityThe mouse monoclonal antibody MEM-216 recognizes an extracellular epitope of CD361 / EVI2B, almost uncharacterized type I transmembrane protein with broad leukocyte expression, mostly in myeloid and B cells.Application detailsFlow cytometry: Recommended dilution: 2 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
TR9
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
CD352, also known as SLAMF6 (SLAM family member 6) is a type I transmembrane glycoprotein expressed on NK cells, T cells, and B cells, and serves as a coreceptor for them. Besides association of its tyrosine phosphorylated intracellular domain with SH2D1A protein, it associates also with SH2 domain-containing phosphatases, which can modulate the signaling. Multiple CD352 isoforms have been identified.SpecificityThe antibody TR9 reacts with an extracellular epitope of CD36 (GPIIIb), a 85 kDa integral membrane glycoprotein expressed on platelets, macrophages, endothelial cells, early erythroid cells and megakaryocytes. The antibody TR9 cross-blocks binding of FITC-labeled standard antibody OKM5. Anti-CD36 antibodies inhibit adhesive functions (e.g. adherence of infected erythrocytes to target cells).Application detailsFlow cytometry: Recommended dilution: 1-12 µg/ml
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
hsF6.4.20
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD35 (complement receptor 1, CR1) is a monomeric multiple modular cell surface glycoprotein which serves as receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. It is expressed mainly on the surface of granulocytes, monocytes, erythrocytes, B cells and folicular dendritic cells. Besides its role in complement cascade, CD35 is involved in blocking BCR-induced proliferation and the differentiation of B cells to plasmablasts and their Ig production.SpecificityThe mouse monoclonal antibody hsF6.4.20 recognizes an extracellular epitope of CD352, a transmembrane glycoprotein expressed on NK, T and B cells.Application detailsImmunohistochemistry (paraffin sections): Heat mediated antigen retrieval. <br>Immunohistochemistry (frozen sections): Acetone fixation. <br>Flow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
E11
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD344 (Frizzled class receptor 4) is a G-protein coupled 7-TM protein, predominantly expressed in fetal neuronal progenitor cells, neuronal intestinal cells, as well as in the kidney, lung, brain, and liver. CD344 is important for regulation of cell polarity, proliferation, and tissue development. Defects in CD344 expression, or its mutation, lead e.g. to serious failures in retinal vascularization, defects in cerebellum, progressive hearing loss, or impaired corpora lutea formation and function.SpecificityThe mouse monoclonal antibody E11 recognizes an extracellular epitope of CD35 (CR1), a type I transmembrane glycoprotein expressed on granulocytes, monocytes, B cells, folicular dendritic cells, erythrocytes, NK and T cell subsets, as well as e.g. on glomerulal podocytes.Application detailsFlow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
CH3A4A7
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
The oncoprotein ErbB2/HER2 (human epidermal growth factor receptor 2), also known as Neu or CD340, is a 185 kDa transmembrane tyrosine kinase of cell surface growth factor receptor family. It is present in a wide variety cell types of normal human fetal and adult tissues and is frequently overexpressed in human carcinomas (e.g. in 20-30% cases of breast cancer cells). Activation of ErbB2 triggers intracellular signalling events, which are essential for cell growth and differentiation. In the last 20 years ErbB2 antigen has become very important marker and therapy target in patient care.SpecificityThe mouse monoclonal antibody CH3A4A7 recognizes an extracellular epitope of CD344 (Frizzled 4), a 7-TM protein of G-protein-coupled receptor family, which is a marker for neuronal stem cells.Application detailsFlow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
24D2
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.SpecificityThe mouse monoclonal antibody 24D2 recognizes an extracellular epitope of human CD340 (HER-2), a type I transmembrane protein expressed by mesenchymal stem cells, B-lymphoblastic leukemia cells, subsets of C-ALL blasts, and and various types of carcinomas.Application detailsWestern blotting: Recommended dilution: 1-2 ?g/ml; positive control: Kg-1a, TF-1 cells, non-reducing conditions. <br>Flow cytometry: Recommended dilution: 2 ?g/ml. <br>Immunohistochemistry (paraffin sections): Recommended dilution: 10 ?g/ml; positive tissue: placenta endothelium.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
4H11[APG]
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD334 / FGFR4 (fibroblast growth factor receptor 4), a transmembrane tyrosine kinase, which is expressed in many tissues, such as in lung, kidney, muscle, heart, pancreas, intestine and other, acts as a receptor for several fibroblast growth factors, namely FGF1, FGF2, FGF6, FGF8, and FGF19. Interaction with these growth factors initiates in cell the signaling cascades leading to the mitogenesis and cell differentiation. Presence of CD334 Gly338Arg allele correlates with prognostic parameters in various cancer studies. CD334 plays multiple roles in the organism, including those of muscle regeneration, cholesterol-to-bile acid metabolism, or glucose homeostasis.SpecificityThe mouse monoclonal antibody 4H11[APG] reacts with extracellular class III epitope on CD34, a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells; it is gradually lost on progenitor cells. The antibody 4H11[APG] completely blocks binding of class III antibodies BIRMA K3 and 8G12 on KG1a cell line.Application detailsFlow cytometry: Recommended dilution: 2-6 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
4FR6D3
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia.SpecificityThe mouse monoclonal antibody 4FR6D3 reacts with an extracellular epitope of CD334, the fibroblast growth factor receptor 4, which is an approximately 88 kDa receptor tyrosine kinase expressed in variety of tissues.Application detailsFlow cytometry: Recommended dilution: 1-5 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
HIM3-4
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD328, also known as Siglec-7 or p75/AIRM1, is a 75 kDa type I transmembrane glycoprotein of sialic acid-binding immunoglobulin-like lectin (Siglec) family. CD328 binds to sialylated glycans with alpha2,6 sialyl and alpha2,8 disyalyl residues and mediates sialic acid-dependent cell-cell binding. As it contains in its intracellular part the immunoreceptor tyrosine-based inhibitory motif (ITIM), it serves as an inhibitory receptor, e.g. of NK cells.SpecificityThe antibody HIM3-4 reacts with an extracellular epitope of CD33, a 67 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells; it is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.Application detailsFlow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
6-434
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD326 / EpCAM (also known as ESA, EGP40, EGP-2, KSA1/4, CO17-1A, GA733-2, MOC31, Ber-EP4) is a 40 kDa transmembrane glycoprotein serving as adhesion molecule in the basolateral membranes in a variety of epithelial cells. CD326 mediates calcium-independent homotypic cell-cell adhesions. CD326 over-expression has been detected in many epithelial tumours and is often associated with bad prognosis. It has been used for diagnostics of (pre-) malignancies at early stages.SpecificityThe mouse monoclonal antibody 6-434 recognizes an extracellular epitope of CD328 (Siglec-7), a 75 kDa transmembrane glycoprotein expressed mainly on NK cells, dendritic cells and monocytes.Application detailsImmunohistochemistry (paraffin sections): Recommended dilution: 5-10 ?g/ml; positive control: colon epithelium, antigen retrieval: heat (sodium citrate) + trypsin. <br>Immunocytochemistry: Recommended dilution: 4 ?g/ml. <br>Western blotting: Recommended dilution: 1-4 ?g/ml. <br> Flow cytometry: Recommended dilution: 1-3 ?g/ml. <br>Immunoprecipitation: Recommended dilution: 1-2 ?g / 100-500 ?g of protein in 1 ml lysate.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
VU-1D9
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD324 / E-cadherin is an epithelial cell surface molecule, which provides calcium-dependent homophilic interactions with E-cadherin of another cell. These intaractions take part in morphogenetic programs controlling the maintenance of the structural and functional integrity of epithelia and affect invasive potential of epithelial neoplasms. CD324 / E-cadherin is implicated in cell growth and differentiation, cell recognition, and sorting during developmental morphogenesis, as well as in aggregation-dependent cell survival. CD324 / E-cadherin-mediated cell adhesion system is highly regulated from inside the cell by a number of intracellular signaling pathways.SpecificityThe mouse monoclonal antibody VU-1D9 recognizes an extracellular epitope within EGF-like domain I of CD326 / EpCAM, a marker of epithelial lineages. This antibody strongly stains various normal epithelial cells and carcinomas.Application detailsImmunohistochemistry (frozen sections): Recommended dilution: 4-8 ?g/ml; positive tissue: tonsil. <br>Flow cytometry: Recommended dilution: 5-10 µg/ml
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
67A4
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD32 (FcgammaRII) is a low affinity receptor for aggregated IgG. It is strongly expressed on monocytes, granulocytes, myeloid and myeloblastic cell lines, and weakly on B cells, CD34+ bone marrow cells, and resting and activated platelets. After binding its ligand, CD32 induces IgG-mediated phagocytosis and oxidative burst in monocytes and neutrophils, whereas in B cells it mediates a negative signal. This polymorphic transmembrane glycoprotein is expressed not only in the activating (CD32a) and inhibitory isoform (CD32b), but also in individual variants with differing avidities for IgG subtypes (e.g. the CD32a131R and CD32a131H allotypes).SpecificityThe mouse monoclonal antibody 67A4 recognizes an extracellular epitope of CD324 / E-cadherin, an approximately 100 kDa epithelial cell adhesion molecule, whose detection is important for determination of invasive potential of epithelial neoplasms.Application detailsFlow cytometry: Recommended dilution: 2-6 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
3D3
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD318 (CUB domain containing protein 1) is a complement domains-containing transmembrane glycoprotein, which takes part in early hematopoiesis. It is expressed on CD34+CD133+ bone marrow cells, keratinocytes, and in human colorectal and breast cancers. It is being used as a marker of mesenchymal stem-like cells, neural progenitor cells, and also as an independent marker for the diagnosis of myeloid leukemias.SpecificityThe mouse monoclonal antibody 3D3 recognizes an extracellular epitope of CD32, a 40 kDa polymorphic transmembrane glycoprotein serving as the low affinity receptor for aggregated IgG. This antibody recognizes CD32 isoforms on B cells of all donors, but on platelets, monocytes, and granulocytes of only some donors (131R variant, but not 131H variant).Application detailsFlow cytometry: Recommended dilution: 2-6 µg/ml
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
CUB1
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD314, also known as NKG2D (natural killer receptor G2D) or KLRK1 (killer cell lectin-like receptor subfamily K, member 1), is a homodimeric C-type lectin-like activating receptor and costimulator with type II membrane orientation (C teminus extracellular). CD314 homodimers are associated with DAP10, a membrane adaptor protein that signals similar to CD28 by recruitment of phosphatidylinositol 3-kinase. Engagement of CD314 amplifies antigen-specific T cell responses in CD314-positive T cell populations. In NK cells, CD314 is a primary activating receptor. As CD314 ligands the MHC class-I chain-related proteins A and B (MICA, MICB) and UL16-binding proteins (ULBPs) have been identified.SpecificityThe mouse monoclonal antibody CUB1 recognizes an extracellular epitope of CD318, a type I transmembrane protein involved in early hematopoiesis.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
1D11
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.SpecificityThe mouse monoclonal antibody 1D11 recognizes an extracellular epitope of CD314 / NKG2D, a 42 kDa C-type lectin-like activating receptor expressed by NK cells, gamma/delta T cells, and CD8+ T cells.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml. <br>Western blotting: Non-reducing conditions.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-05
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD307d is a type I transmembrane glycoprotein of the Fc receptor family. It contains two ITIM motifs and one ITSM motif in its cytoplasmic domain. CD307d is expressed mainly on the surface of memory B cells in mucosa-associated lymphoid tissues. It binds to aggregated immunoglobulin molecules (IgA, IgG). Defects of CD307d may play a role in HIV-induced memory B cell dysfunction.SpecificityThe antibody MEM-05 reacts with an extracellular epitope of CD31 (PECAM-1), a 130-140 kDa type I transmembrane glycoprotein expressed on monocytes, platelets, granulocytes, endothelial cells and stem cells of the myeloid lineage.Application detailsFlow cytometry: Recommended dilution: 2-5 ?g/ml.
Antibody Isotype:
IgG2a kappa
Monosan Range:
MONOSAN
Clone:
A1
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD307c is a type I transmembrane glycoprotein of the Fc receptor family. It contains both ITAM and ITIM motifs in its cytoplasmic domain. CD307c is expressed on the surface of NK cells, and T, Treg, B and plasma cell subsets. It seems to play a role in the regulation of immune response. Defects in CD307c function can result in autoimmune diseases, e.g. rheumatoid arthritis or systemic lupus erythematosus.SpecificityThe mouse monoclonal antibody A1 recognizes an epitope within extracellular domain of CD307d, a transmembrane glycoprotein expressed mainly on memory B cells.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG2b kappa
Monosan Range:
MONOSAN
Clone:
H5
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD307b is a type I transmembrane glycoprotein of the Fc receptor family. It contains one ITAM motif and two ITIM motifs in its cytoplasmic domain. It is expressed in spleen and lymph nodes in mature B cells and memory B cells. CD307b may be a prognostic marker for chronic lymphocytic leukemia.SpecificityThe mouse monoclonal antibody H5 recognizes an epitope within extracellular part of CD307c, a transmembrane glycoprotein expressed mainly on NK cells, and T and B cell subsets.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG2a kappa
Monosan Range:
MONOSAN
Clone:
B24
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD307a is a type I transmembrane glycoprotein of the Fc receptor family. It contains two ITAM motifs in its cytoplasmic domain. CD307a is expressed mainly on the surface of mature B-cells, and is down-regulated in germinal center B-cells. Expression of CD307a is higher in patients with autoimmune diseases, compared with healthy controls.SpecificityThe mouse monoclonal antibody B24 recognizes an extracellular epitope of CD307b, a transmembrane glycoprotein expressed mainly in mature and memory B cells.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
E3
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD305, also known as LAIR1 (leukocyte-associated Ig-like receptor 1), is an inhibitory receptor found on many types of peripheral blood cells. It serves to suppress cell cytotoxicity, activation, proliferation, and differentiation regarding autoantigens via its two intracellular ITIM sites. CD305 belongs to the immunoglobulin superfamily and the leukocyte-associated inhibitory receptor family of proteins. It reacts with collagen ligands.SpecificityThe mouse monoclonal antibody E3 recognizes an extracellular epitope of CD307a, a transmembrane glycoprotein expressed predominantly on the surface of mature B cells.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
NKTA255
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD300e / IREM-2 (immune receptor expressed by myeloid cells 2), also known as CLM2 or LMIR6, is a monomeric transmembrane glycoprotein with a single extracellular immunoglobulin-like domain. Intracellularly it associates with DAP-12, an ITAM-containing adaptor molecule. CD300e is expressed on mature monocytes and peripheral blood myeloid dendritic cells. Its crosslinking leads to release of pro-inflammatory cytokines, and increased expression of activation markers.SpecificityThe mouse monoclonal antibody NKTA255 recognizes an extracellular epitope of CD305 / LAIR1, a 40 kDa type I transmembrane glycoprotein expressed on NK, T, and B cells, monocytes, dendritic cells, eosinophils, basophils, mast cells, CD34+ hematopoietic progenitor cells and thymocytes.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
UP-H2
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD30 is a type I transmembrane glycoprotein of the TNF receptor superfamily. CD30 was originally identified as a cell surface antigen of Hodgkins and Reed-Sternberg cells using monoclonal antibody Ki-1. The ligand for CD30 is CD30L (CD153). The binding of CD30 to CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation, and apoptotic cell death. CD30 has a critical role in the pathophysiology of Hodgkin's disease and other CD30+ lymphomas. CD30 acts as a costimulatory molecule in thymic negative selection. In addition to its expression on Hodgkin's and Reed-Sternberg cells, CD30 is also found in some non-Hodgkin's lymphomas (including Burkitt's lymphomas), virus-infected T and B cells, and on normal T and B cells after activation. In T cells, CD30 expression is present on a subset of T cells that produce Th2-type cytokines and on CD4+/CD8+ thymocytes that co-express CD45RO and the IL4 receptor. Soluble form of CD30 (sCD30) serves as a marker reflecting Th2 immune response.SpecificityThe mouse monoclonal antibody UP-H2 recognizes an extracellular epitope on CD300e / IREM-2, a 32 kDa glycoprotein expressed by mature monocytes and peripheral blood myeloid dendritic cells.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml. <br>Immunohistochemistry: Recommended dilution: 5-10 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
Ber-H8
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkynje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.SpecificityThe mouse monoclonal antibody Ber-H8 recognizes extracellular part of CD30 (Ki-1 antigen), a 105 kDa single chain glycoprotein expressed on Hodgkin's and Reed-Sternberg cells; it is also found in Burkitt's lymphomas, virus-infected T and B lymphocytes, and on normal B and T lymphocytes after activation (T lymphocytes that produce Th2-type cytokines and on CD4+/CD8+ T lymphocytes that co-express CD45RO and the IL4 receptor).Application detailsFlow cytometry: Recommended dilution: 1-5 µg/ml
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
MEM-92
Concentration:
1 mg/ml
Format:
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Storage buffer:
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.SpecificityThe antibody MEM-92 reacts with an extracellular epitope on epsilon chain of human CD3 complex, a part of a bigger multisubunit complex of the T cell receptor (CD3/TCR) expressed on peripheral blood T lymphocytes and mature thymocytes.Application detailsFlow cytometry: Recommended dilution: 1-12 µg/ml
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
CD28.2
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD279 / PD-1 (programmed cell death 1), a transmembrane protein of CD28/CTLA-4 family. It is expressed inducibly mainly on activated T, B, and myeloid cells and plays a role in maintaining peripheral self-tolerance. Binding to its ligands CD273 and CD274 is associated with inhibition of T cell proliferation and induction of their anergy. It is also expressed during thymic development. Some variants of CD279 are associated with susceptibility to systemic lupus erythematosus, type 1 diabetes, and rheumatoid arthritis.SpecificityThe mouse monoclonal antibody CD28.2 recognizes an extracellular epitope of CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
EH12.2H7
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD274 / PD-L1 (programmed death ligand-1), also known as B7-H1, is a member of the B7 family of regulatory proteins. It can act as both costimulatory and coinhibitory molecule for T cells. Interaction with its receptor CD279 (PD1) appears to be important in the maintenance of peripheral tolerance and in prevention of tumor rejection. Even pathogens (e.g. Schistosoma) may exploit CD274 to evade an immune response. Besides CD279, existence of other receptor(s) for CD274 is likely.SpecificityThe mouse monoclonal antibody EH12.2H7 recognizes an extracellular epitope of CD279 / PD-1 (programmed cell death 1), a 55 kDa type I transmembrane protein expressed above all during T cell development, on activated T cells, activated B cells, and activated monocytes.Application detailsImmunohistochemistry (frozen sections): Acetone fixation. <br>Flow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG2b kappa
Monosan Range:
MONOSAN
Clone:
29E.2A3
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD273 / PD-L2 (programmed death ligand-1), also known as B7-DC, is a member of the B7 family of regulatory proteins. It costimulates the proliferation of T cells, and mediates IFN gamma production. Ligation of CD273 on dendritic cells enhances dendritic cell activation and T cell responses. When interacting with CD279, it can act as a coinhibitor of the T cell function. CD273 expression is a useful marker to distinguish primary mediastinal B cell lymphoma from other diffuse large B cell lymphomas.SpecificityThe mouse monoclonal antibody 29E.2A3 recognizes an extracellular epitope of CD274 / PD-L1 (also known as B7-H1), a 40 kDa type I transmembrane protein expressed by dendritic cells, activated T cells, activated monocytes, and in various tissues, above all in heart and skeletal muscle, placenta and lung, and in many cancer cells, including T cell lymphomas, melanomas, and glioblastomas.Application detailsFlow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG2a kappa
Monosan Range:
MONOSAN
Clone:
24F.10C12
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD272, a type I transmembrane glycoprotein, contains in its intracellular domain two ITIM sequences, which are upon CD272 triggering phosphorylated and recruit SHP phosphatases to attenuate cell activation. CD272 is expressed on B and T lymphocytes, NK cells, dendritic cells, and macrophages, and its ligand is CD270. Defects in CD272-CD270 inhibitory mechanism lead to autoimmune diseases. Overexpression of CD272 is a marker of tolerant T cells.SpecificityThe mouse monoclonal antibody 24F.10C12 recognizes an extracellular epitope of CD273 / PD-L2 (also known as B7-DC), a 25 kDa type I transmembrane protein expressed by dendritic cells, activated monocytes and T cells, heart, first trimester placenta, lung and liver, as well as in Hodgkin´s lymphoma cells and primary mediastinal B cell lymphoma (PMBL).Application detailsFlow cytometry: Recommended dilution: 2-5 ?g/ml.
Antibody Isotype:
IgG2a kappa
Monosan Range:
MONOSAN
Clone:
MIH26
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD271 / NGFR, also known as p75NGFR or p75NTR, is a 75 kDa low affinity receptor for the NGF (nerve growth factor), BDNF (brain-derived growth factor), and other neurotrophins, such as NT3 and NT4/5. Unlike other members of the tumor necrosis factor receptor superfamily of transmembrane proteins, CD271 has unique intracellular domain structure (lacks catalytic activity) and downstream signaling partners. Triggered by its ligands CD271 affects growth, differentiation, migration and death of the nervous system cells.SpecificityThe mouse monoclonal antibody MIH26 recognizes an extracellular epitope of CD272, a transmembrane glycoprotein serving as a negative regulator of the activation in various leukocyte types.Application detailsImmunohistochemistry (paraffin sections): Recommended dilution: 1-2 ?g/ml; Positive tissue: melanoma, heat-mediated antigen retrieval. <br>Flow cytometry: Extracellular and intracellular staining; recommended dilution: 1-4 µg/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
NGFR5
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD268 / BAFF R is a TNFR family receptor that binds the B-cell-activating factor (CD257 / BAFF). Splice variants of CD268 have been observed both in man and mouse. A naturally occurring mutation of CD268 in A/WySnJ mice is associated with low number of mature B cells, but with normal B cell precursors. The role of BAFF in B-cell survival and activation make CD268 a potential diagnostic reagent. It may be involved in survival of B-cell malignancies. Experimental administration of a CD268-Fc fusion protein suppresses antibody responses. In T cells the CD268 costimulates their activation and proliferation. Defects in CD268 cause the common variable immunodeficiency 4 (CVID4).SpecificityThe mouse monoclonal antibody NGFR5 (originally C34C) recognizes an epitope within ammino acids 1 - 160 of CD271/NGFR, a 75 kDa transmembrane glycoprotein of the TNFR superfamily.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
11C1
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD266 / TWEAK R (TNFRSF12A), also known as FN14 (fibroblast growth factor-inducible 14) is a receptor for CD255 / TWEAK, the TNF-like weak inducer of apoptosis. CD266 is expressed on endothelial cells, as well as on some cancer tissues, and plays a role in CD255-induced endothelial cell migration, proliferation, and angiogenesis. The CD255-CD266 interaction, or antibody-mediated triggering of CD266 is also able to induce apoptosis and necrosis in CD266-positive cells (including tumor cells), which might have therapeutic potential.SpecificityThe mouse monoclonal antibody 11C1 recognizes an extracellular epitope of CD268 / BAFF R (B cell-activating factor receptor), a 19 kDa type III transmembrane protein expressed on resting B cells and CD4-positive T cells, but down regulated after activation.Application detailsFlow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
ITEM-4
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
TRAIL-R1 (CD261, DR4) is a type I transmembrane protein, also called TRAIL receptor 1. The ligand for this DR4 death receptor has been identified and termed TRAIL, which is a member of the TNF family. DR4, as many other receptors (Fas, TNFR1, etc.), mediates apoptosis and NF kappaB activation in many cells and tissues. Apoptosis, a programmed cell death, is a operating process in normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by coupled of certain cytokines (TNF family - TNF, Fas ligand) and their death domain containing receptors (TNFR1, Fas receptor).SpecificityThe mouse monoclonal antibody ITEM-4 recognizes an extracellular epitope of CD266 / TWEAK R, a TNFR superfamily receptor for CD255 / TWEAK, a TNF-like weak inducer of apoptosis.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
DR-4-02
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD26, also known as dipeptidyl peptidase IV (DPP-IV), is a homodimeric cell surface serine peptidase that degradates IFN-gamma-induced cytokines, acts as a T cell costimulatory molecule, and participates in multiple immunopathological roles in leukocyte homing and inflammation. Alterations in its peptidase activity are characteristic of malignant transformation. The enzymatic activity increases dramatically with tumour grade and severity. CD26 is expressed in various blood cell types, but also e.g. in cells that are histogenetically related to activated fibroblasts. Alterations in CD26 density have been reported on circulating monocytes and CD4+ T cells during rheumatoid arthritis and systemic lupus erythematosus.SpecificityThe mouse monoclonal antibody DR-4-02 recognizes an extracellular epitope of TRAIL-R1 (DR4), a human death receptor 4 expressed in most human tissues (spleen, peripheral blood leucocytes, thymus) and in a variety of tumour-derived cell lines.Application detailsFlow cytometry: Recommended dilution: 1-2 ?g/ml; positive control: human peripheral blood leukocytes.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
BA5b
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD255 / TWEAK (TNF-related weak inducer of apoptosis), a type II transmembrane protein expressed as membrane-bound and secreted form, can induce apoptosis in many tissues and cell lines through its receptor CD266 / TWEAK R. On the other hand, in endothelial cells this interaction can induce proliferation and promote angiogenesis including neovascularization of tumours. CD255 can act in a juxtacrine manner to initiate cellular responses, and induces secretion of pro-inflammatory cytokines. Besides CD266, CD255 may also bind to DR3.SpecificityThe mouse monoclonal antibody BA5b recognizes an extracellular epitope of CD26, a 110 kDa type II transmembrane glycoprotein, which is a peptidase expressed on mature thymocytes, T cells (especially activated), B cells, NK cells and macrophages.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG3 kappa
Monosan Range:
MONOSAN
Clone:
CARL-1
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Human CD253 / TRAIL (TNF-related apoptosis inducing ligand), also called Apo2, is a type II membrane protein from the TNF family. TRAIL is a cytotoxic protein which activates rapid apoptosis in tumor cells, but not in normal cells. TRAIL-induced apotosis, is achieved through binding to two dealth-signaling receptors, DR4 (CD261 / TRAIL-R1) and DR5 (CD262 / TRAIL-R2).SpecificityThe mouse monoclonal antibody CARL-1 recognizes an extracellular epitope of CD255 / TWEAK, a type II transmembrane protein of the TNF superfamily able to induce apoptosis weakly in many cell types.Application detailsFlow cytometry: Recommended dilution: 1-5 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
2E5
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. Humanized anti CD25 antibodies represent a useful tool to reduce the incidence of allograft rejection as well as the severity of graft versus host reaction, and radioimmunoconjugates of anti-CD25 antibodies can be used against CD25 expressing lymphomas.SpecificityThe antibody 2E5 reacts with an extracellular epitope within C-terminal half of TRAIL (APO-2L), a 21 kDa cytotoxic protein, activator of rapid apoptosis in tumor cells. TRAIL is mainly expressed in spleen, lung, prostate and also in many other tissues.Application detailsFlow cytometry: Recommended dilution: 2 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-181
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD243, also known as multidrug resistant protein 1 (MDR-1) or P-glycoprotein (Pgp) is an ATP binding cassette (ABC)-containing efflux transporter for xenobiotic lipophilic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. It is expressed in many tissues, including the brain, liver, pancreas, testes, kidney, and blood (B, T, NK cells, but not monocytes).SpecificityThe antibody MEM-181 reacts with an extracellular epitope of CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.Application detailsImmunohistochemistry (paraffin sections): Heat mediated antigen retrieval in citrate buffer pH 6. <br>Flow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG2a kappa
Monosan Range:
MONOSAN
Clone:
UIC2
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD24, also known as heat-stable antigen (HSA) or nectadorin, is a small mucin-like GPI-anchored extracellular membrane glycoprotein expressed on several cell types, including B cells. When B cells are activated and induced to further maturation, however, CD24 begins to disappear. CD24 seems to act as a gate-keeper for lipid rafts, thereby regulating the activity of integrins and other proteins such as the chemokine receptor CXCR4; it is also a ligand for P-selectin. CD24 triggering induces apoptosis of B cell precursors but not in mature resting B cells, where it instead inhibits their ability to proliferate in response to activation. CD24 expression is associated with invasiveness and poorer prognosis of carcinomas and is a marker of exosomes secreted into urine and amniotic fluid.SpecificityThe mouse monoclonal antibody UIC2 recognizes an extracellular epitope on CD243 (MDR-1), an approximately 170 kDa ABC transporter expressed on hematopoietic stem cells, B, T, and NK cells, or on many multidrug resistant cancer cells. This antibody preferentially recognizes CD243 in the process of transporting substrate.Application detailsImmunohistochemistry (frozen sections): Positive tissue: tonsil. <br>Flow cytometry: Recommended dilution: 1-12 µg/ml
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
SN3
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD235a (Glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to glycophorin B (GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, an Streptococcus adhesin.SpecificityThe antibody SN3 reacts with CD24, a 35-45 kDa heavily glycosylated cell surface antigen. CD24 is expressed by granulocytes, B lymphocytes and by some activated T cells and T cell malignancies. It is not expressed on human thymocytes.Application detailsImmunohistochemistry (paraffin sections): Recommended dilution: 10 ?g/ml. <br>Flow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
JC159
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD231 (TALLA-1, T-ALL-asociated antigen 1), also known as tetraspanin 7, is a 150 kDa (under reducing conditions 32-45 kDa) transmembrane glycoprotein of tetraspanin family, expressed in T-type acute lymphoblastic leukemia, neuroblastoma, and neuronal tissue. Mutations of CD231 gene are associated with X-linked mental retardation, Huntington´s chorea, and myotonic dystrophy. CD231 interacts with integrins and may have a role in the control of neurite outgrowth. Antibodies to CD231 are important for detection of T-ALL and are potential targets of its treatment.SpecificityThe mouse monoclonal antibody JC159 recognizes an epitope between amino acids 27 and 40 of the extracellular portion of CD235a (glycophorin A), a sialoglycoprotein expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL. The antibody does not react with glycophorin B.Application detailsImmunohistochemistry: Positive control: brain. <br>Flow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
B2D
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD229 (Ly9) is a cell surface receptor of the CD150 family, which includes also e.g. CD48 and CD224. Receptors of this family regulate cytokine production and cytotoxicity of lymphocytes and NK cells. High levels of CD229 are found on T and B cells, where its expression increases during their maturation. It is absent on granulocytes, bone marrow-derived dendritic cells, platelets and erythrocytes. CD229 has been also reported on mouse monocytes and NK cells. CD229 interacts homophilically through its N-terminal domain and localizes to the contact site between T cells and antigen presenting B cells during antigen-dependent immune synapse formation.SpecificityThe mouse monoclonal antibody B2D recognizes an extracellular epitope of CD231 (TALLA-1, tetraspanin 7), a transmembrane glycoprotein expressed in neuronal tissue and T-ALL.Application detailsFlow cytometry: Recommended dilution: 6 ?g/ml; positive control: peripheral blood lymphocytes.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
HLy9.25
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD222 (CIMPR, cation-independent mannose 6-phosphate receptor; IGF2 receptor) is a ubiquitously expressed 250 kDa transmembrane protein. No more than 10% of CD222 is present on the cell surface where it serves as a multifunctional receptor. Intracellular (major) fraction of CD222 is involved in transport of newly synthesized lysosomal enzymes modified by mannose 6-phosphate from Golgi apparatus to lysosomes. The cell surface CD222 binds and internalizes exogeneous mannose 6-phosphate-containing ligands. Importantly, CD222 is crutial for internalization and degradation of insulin-like growth factor 2, thus controling cell growth. CD222 also complexes CD87 (urokinase-type plasminogen-activator receptor), plasminogen and latent TGF-beta, last but not least CD222 serves as a receptor for heparanase and even for Listeria.SpecificityThe mouse monoclonal antibody HLy9.25 (also known as HLy9.1.25) recognizes an extracellular epitope of CD229 / Ly9, a 100-120 kDa cell surface glycoprotein expressed on T and B cells.Application detailsFlow cytometry: Extracellular and intracellular staining. Recommended dilution: 2-6 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-240
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD218a, an approximately 62 kDa type I transmembrane glycoprotein, is the alpha subunit of IL-18 receptor heterodimer. It is expressed in various immune cells, including lymphocytes, NK cells, and myeloid cells, as well as in heart, lung, liver, gut and another tissues. IL-18 is a proinflammatory cytokine, that promotes both Th1 and Th2 immune responses, induces IFN-gamma production and activates NK cells.SpecificityThe antibody MEM-240 recognizes an extracellular epitope between amino acids 698-1262 of CD222 (IGF2 receptor), a ubiquitously expressed 250 kDa multifunctional type I transmembrane protein. The majority of CD222 is found in the late endosomal/prelysosomal compartment, 5-10% in the plasma membrane and the truncated (220 kDa) form of CD222 is present in human and bovine serum.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
H44
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD21 (complement receptor 2, CR2) binds C3 complement fragments, especially its breakdown fragments, which remain covalently attached to complement activating surfaces or antigen. CD21 has important roles in uptake and retention of immunocomplexes, survival of memory B cells and in development and maintenance of the humoral response to T-dependent antigens. CD21 also serves as a key receptor for Epstein-Barr virus binding and is involved in targeting prions to folicular dendritic cells and expediting neuroinvasion following peripheral exposure to prions. A soluble form of the CD21 (sCD21) is shed from the lymphocyte surface and retains its ability to bind respective ligands.SpecificityThe mouse monoclonal antibody H44 recognizes an extracellular epitope of CD218a, the alpha subunit of IL-18 receptor.Application detailsFlow cytometry: Recommended dilution: 2 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
LT21
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD209, also known as DC-SIGN (dendritic cell-specific ICAM-3-grabbing nonintegrin) is a transmembrane receptor expressed on the surface of dendritic cells and macrophages, which recognizes numerous pathogens ranging from parasites to viruses. Its N-terminal domain is transmembrane, whereas a tandem-repeat neck domain and the C terminal C-type lectin carbohydrate recognition domain have dual function as a pathogen recognition receptor and a cell adhesion receptor. The neck region is responsible for homo-oligomerization which allows the receptor to bind multivalent ligands with high avidity. A ligand of CD209 is also CD50 (ICAM-3).SpecificityThe antibody LT21 reacts with an extracellular epitope of CD21 (CR2), a 145 kDa transmembrane glycoprotein (complement C3d receptor - C3dR) expressed on B lymphocytes, follicular dendritic cells, some epithelial cells and a subsets of T lymphocytes. It is not expressed on immature B cells.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
UW60.1
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD205, also known as DEC-205, is an endocytic receptor of macrophage mannose receptor family. This 205 kDa C-type lectin transmembrane protein mediates adsorptive uptake and its intracellular domain contains coated pit localization sequence and distal acidic motif, which is required for recycling beyond early endosomes through deeper MHC II+ late endosomes and lysosomes. This unique pathway of receptor-mediated uptake proves to be necessary for presentation of antigenic peptides at low doses of ligand. CD205 is responsible for uptake and processing of captured antigens for dendritic cells.SpecificityThe mouse monoclonal antibody UW60.1 recognizes an extracellular epitope of human CD209 (DC-SIGN), a 44 kDa transmembrane receptor, expressed on the surface of dendritic cells and macrophages.Application detailsWestern blotting: Only non-denatured samples. <br>Flow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
HD30
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD203c, also known as ENPP-3, is integral membrane ectoenzyme (ectonucleotide pyrophosphatase/phosphodiesterase 3), that hydrolyses nucleotide triphosphates and thus modulates purinergic signaling. CD203c is expressed mainly on activated basophils and mast cells. CD203c is upregulated in response to IgE-receptor cross-linking and is overexpressed on neoplastic mast cells in patients with systemic mastocytosis. Measurement of its induced enhancement on the plasma membrane is useful for diagnostics of allergies.SpecificityThe mouse monoclonal antibody HD30 recognizes an extracellular epitope of CD205, an approx. 200 kDa C-type lectin transmembrane protein of the MMR (macrophage mannose receptor) family, expressed at high levels on dendritic cells and thymic epithelial cells, and at low levels on lymphocytes, NK cells and monocytes.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml. Extracellular and intracellular staining.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
NP4D6
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD200R is a transmembrane glycoprotein, expressed on the surface of myeloid cells. Its interaction with CD200 leads in these cells to a downregulatory signal. This interaction may control myeloid function in a tissue-specific manner. Alternative splicing of CD200R gene results in multiple transcript variants. These isoforms may play a role in differentiation, e.g. regards tolerogenic dendritic cells. Besides myeloid cells, CD200R can be found also on a T cell subset.SpecificityThe mouse monoclonal antibody NP4D6 reacts with an extracellular epitope of CD203c, a transmembrane ectoenzyme expressed on basophils and mast cells, and overexpressed upon their activation.Application detailsFlow cytometry: Recommended dilution: 2-5 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
OX-108
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD200 (also known as OX2 or MRC) is a type-1 membrane glycoprotein, which contains two extracellular immunoglobulin domains, transmembrane domain and cytoplasmic domain. It is expressed by neuronal cells, B and T cell subsets, follicular dendritic cells, keratinocytes, and ovarian cells. The interaction between CD200 and its receptor CD200R results in macrophage activation (IL-6 production), inhibition of mast cell degranulation along with reduced TNF-alpha and IL-13 secretion and overall attenuation of the activation status of lymphocytes. It seems CD200 is also involved in maternal tolerance and its decreased expression in hair follicle correlates with follicular miniaturization.SpecificityThe mouse monoclonal antibody OX-108 recognizes an extracellular epitope on human CD200R, a transmembrane flycoprotein expressed on the surface of myeloid cells.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
OX-104
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD20 is a cell surface 33-37 (depending on the degree of phosphorylation) kDa non-glycosylated surface phosphoprotein expressed on mature and most malignant B cells, but not stem cells or plasma cells (low number of the CD20 has been also detected on a subpopulation of T lymphocytes and it can be expressed on follicular dendritic cells). Its expression on B cells is synchronous with the expression of surface IgM. CD20 regulates transmembrane calcium conductance (probably functioning as a component of store-operated calcium channel), cell cycle progression and B-cell proliferation. It is associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following mAb treatment.SpecificityThe mouse monoclonal antibody OX-104 recognizes an extracellular epitope of CD200, a type-1 glycoprotein of the immunoglobulin superfamily, which is expressed in neurons, B and T cell subsets, keratinocytes, follicular dendritic cells, and ovarian cells.Application detailsFlow cytometry: Recommended dilution: 3 ?g/ml.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
LT20
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD2 belongs to T lymphocyte glycoproteins of immunoglobulin superfamily. Its interaction with CD58 stabilizes adhesion between T cells and antigen presenting or target cells. Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion. Moreover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58. Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion. CD2 deficiency reduces intestinal inflammation and helps to control infection.SpecificityThe antibody LT20 reacts with an extracellular epitope of CD20 (Bp35), a 33-37 kDa non-glycosylated membrane receptor with four transmembrane domains, expressed on B lymphocytes (it is lost on plasma cells), follicular dendritic cells, and at low levels on peripheral blood T lymphocytes.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-65
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD1d belongs to CD1 family of transmembrane glycoproteins, associated with beta2 microglobulin, similarly to MHC I molecules. Unlike other CD1 family members, however, CD1d can be also expressed in a non-glycosylated form, which is not associated with beta2 microglobulin. Hence it is not certain how much CD1d plays a role in the presentation of microbial lipid antigens during infection. On the other hand, it is expressed on various antigen presenting cell types. Besides it, CD1d+ thymocytes are involved in the positive selection of sublineage of NKT cells.SpecificityThe antibody MEM-65 recognizes a unique extracellular epitope of CD2, a 50 kDa glycoprotein present on the human peripheral blood T-lymphocytes and NK cells; also expressed by all thymocytes.Application detailsFlow cytometry: Recommended dilution: 4-8 ?g/ml.
Antibody Isotype:
IgG2b kappa
Monosan Range:
MONOSAN
Clone:
51.1
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD1c (also known as R7 or BDCA1) together with CD1a and b, belongs to group 1 of CD1 antigens. These non-classical MHC-like glycoproteins serve as antigen-presenting molecules for a subset of T cells that responds to specific lipids and glycolipids found in the cell walls of bacterial pathogens or self-glycolipid antigens such as gangliosides, and they have also roles in antiviral immunity. The trafficking routes of the particular CD1 types differ and correspond to their ability to bind and present different groups of antigens. CD1c is unique in its ability to present e.g. mycobacterial phosphoketides and polyisoprenoids. CD1c is the only CD1 isoform that has been shown to interact both with alpha/beta and gamma/delta T cells.SpecificityThe mouse monoclonal antibody 51.1 recognizes an extracellular epitope of CD1d, a 38 kDa transmembrane glycoprotein expressed mainly on cortical thymocytes, marginal zone B cells and other antigen presenting cells, but also i e.g. hepatitis C virus-infected livers.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
L161
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD1b (also known as R1) together with CD1a and c, belongs to group 1 of CD1 antigens. These non-classical MHC-like glycoproteins serve as antigen-presenting molecules for a subset of T cells that responds to specific lipids and glycolipids found in the cell walls of bacterial pathogens or self-glycolipid antigens such as gangliosides, and they have also roles in antiviral immunity. The trafficking routes of the particular CD1 types differ and correspond to their ability to bind and present different groups of antigens. Besides non-peptide glycolipid antigen presentation to CD1-restricted T cells, CD1b has been implicated in thymocyte development.SpecificityThe mouse monoclonal antibody L161 recognizes an extracellular epitope of CD1c, (R7), a 43 kDa type I glycoprotein associated with beta2-microglobulin. It is expressed on cortical thymocytes (strongly), Langerhans cells, dendritic cells, B and some T cells.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
SN13
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD1a, together with CD1b and c, belongs to group 1 of CD1 glycoproteins. These proteins serve as antigen-presenting molecules for a subset of T cells that responds to specific lipids and glycolipids found in the cell walls of bacterial pathogens or self-glycolipid antigens such as gangliosides, and they have also roles in antiviral immunity. Unlike CD1b, CD1a is excluded from late endosomal compartments and instead traffics independently in the recycling pathway of the early endocytic system, and CD1a antigen presentation is independent upon vesicular acidification.SpecificityThe mouse monoclonal antibody SN13 (also known as K5-1B8) recognizes an extracellular epitope of CD1b, a 44 kDa type I glycoprotein associated with beta2-microglobulin. It is expressed on dendritic cells, Langerhans cells, thymocytes, and T acute lymphoblastic leukemia cells.Application detailsFlow cytometry: Recommended dilution: 1-5 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
HI149
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD199 (CCR9) is a G-protein-coupled 7 TM chemokine receptor for TECK (SCYA25) chemokine. It is expressed strongly in thymus, at lower level in bone marrow and spleen, as well as on a subset of memory T cells specialized for mucosal homing. CD199 appears to confer homing properties to the small intestine on memory T cells. On the other hand it functions as a coreceptor for HIV-1.SpecificityThe antibody HI149 reacts with an extracellular epitope of CD1a (T6), a 49 kDa polypeptide associated with beta2-microglobulin expressed on cortical thymocytes (strongly), Langerhans cells, dendritic cells and some T cell leukemias and lymphomas. The antibody does not react with peripheral blood T and B lymphocytes, monocytes, granulocytes, platelets and erythrocytes.Application details
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
C9Mab-1
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD195 / CCR5 (also known as CKR-5) is a receptor for inflammatory CC-chemokines (characterized by a pair of adjacent cysteine residues), such as MIP-1 alpha, MIP-1 beta, or RANTES. It is a G protein-associated seven-pass transmembrane protein expressed on resting T cells with memory/effector phenotype, monocytes, macrophages and immature dendritic cells. This chemokine receptor regulates the activation and directed migration of leukocytes. Importantly, along with CD4, CD195 / CCR5 functions as a major receptor for HIV. Their ligand is the viral glycoprotein gp120.SpecificityThe mouse monoclonal antibody C9Mab-1 recognizes an extracellular epitope of CD199, a 7-transmembrane chemokine receptor.Application detailsWestern blotting: Do not boil cell lysates prior to SDS-PAGE. <br>Flow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
T21/8
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD193 / CCR3 is a G-protein coupled receptor for several chemokines, namely CCL11 (eotaxin), CCL26 (eotaxin-3), CCL7 (MCP-4), or CCL5 (RANTES). It is highly expressed on eosinophils and basophils, and is also detected in TH1 and TH2 cells, as well as in airway epithelial cells. CD193 is the key eosinophil chemokine receptor responsible for regulation of eosinophil migration and function. This receptor may contribute to the accumulation and activation of eosinophils and other inflammatory cells in the allergic airway. It is also known to be an entry co-receptor for HIV-1.SpecificityThe mouse monoclonal antibody T21/8 recognizes an extracellular epitope on the N-teminus of CD195, an approximately 45 kDa G-protein coupled receptor 1 family protein expressed on resting T cells, monocytes, macrophages, and immature dendritic cells.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG2b kappa
Monosan Range:
MONOSAN
Clone:
5E8
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD19 is a transmembrane glycoprotein of Ig superfamily expressed by B cells from the time of heavy chain rearrangement until plasma cell differentiation. It forms a tetrameric complex with CD21 (complement receptor type 2), CD81 (TAPA-1) and Leu13. Together with BCR (B cell antigen receptor), this complex signals to decrease B cell treshold for activation by the antigen. Besides being signal-amplifying coreceptor for BCR, CD19 can also signal independently of BCR coligation and it turns out to be a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.SpecificityThe mouse monoclonal antibody 5E8 recognizes an extracellular epitope of CD193 (chemokine receptor 3), an approximately 41 kDa protein expressed above all in eosinophils and basophils.Application detailsFlow cytometry: Recommended dilution: 5 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
LT19
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD187 (CXCR7) is a member of chemokine receptor family, but with discussed specificity. It is expressed in various tissues and cells, such as placenta, urinary bladder, fetal liver cells, tumor cells, activated endothelium, monocytes, lymphocytes, mature dendritic cells, and other.SpecificityThe antibody LT19 reacts with an extracellular epitope of CD19 (B4), a 95 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on B lymphocytes and follicular dendritic cells; it is lost on plasma cells.Application detailsFlow cytometry: Recommended dilution: 1-12 µg/ml
Antibody Isotype:
IgG2a kappa
Monosan Range:
MONOSAN
Clone:
10D1-J16
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD184, also known as CXCR4 or fusin, is a receptor for the C-X-C chemokine SDF-1. It is expressed mainly in hematopoietic cells, vascular endothelium, and neural tissue. CD184 is a G-protein coupled receptor containing extracellular N-terminal, seven transmembrane domains and intracellular C-terminal domain. It transduces signal by increasing the intracellular calcium level. CD184 plays an essential role in vascularization of the gastrointestinal tract, and is involved in cerebellar development and in hematopoiesis. It is also a coreceptor (with CD4) for HIV-1 X4 virus and a primary receptor for some HIV-2 isolates.SpecificityThe mouse monoclonal antibody 10D1-J16 recognizes an extracellular epitope on CD187/CXCR7, a transmembrane protein of chemokine receptor family.Application detailsFlow cytometry: Recommended dilution: 2-4 ?g/ml.
Antibody Isotype:
IgG2a kappa
Monosan Range:
MONOSAN
Clone:
12G5
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD180, also known as RP105 (or Bgp95, LY64) is a type I membrane glycoprotein of Toll-like receptor (TLR) family. Its cytoplasmic tail is short and unlike the TLRs, it lacks the TIR domain. CD180 expression is dependent on the coexpression of its helper molecule, MD-1, and mirrors that of TLR4 on antigen-presenting cells. CD180 regulates recognition of LPS and signaling in B cells, via interacting directly with the TLR4 signaling complex, inhibiting its ability to bind microbial ligands. Ligation of CD180 by monoclonal antibodies leads to B cell activation, upregulation of CD80/CD86, and increase in cell size.SpecificityThe mouse monoclonal antibody 12G5 recognizes an extracellular epitope of CD184, a 45 kDa G-protein-linked CXC chemokine receptor widely expressed on blood and tissue cells.Application detailsFlow cytometry: Recommended dilution: 2 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
G28-8
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.SpecificityThe mouse monoclonal antibody G28-8 recognizes an extracellular epitope of CD180, a 95-105 kDa TLR-like glycoprotein expressed on peripheral blood monocytes and dendritic cells, mantle zone B cells and marginal zone B cells, but very weakly on germinal center B cells.Application detailsWestern blotting: Recommended dilution: 2 ?g/ml; positive control: Kg-1a human leukemia cell lysate, non-reducing conditions. <br>Flow cytometry: Recommended dilution: 2 ?g/ml. <br>Immunohistochemistry (paraffin sections): Recommended dilution: 10 ?g/ml; positive tissue: spleen, microglia.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-48
Concentration:
1 mg/ml
Format:
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD178 / Fas-L (Fas ligand, CD95L), a member of TNF family transmembrane glycoproteins, is responsible for induction of apoptosis in cells containing its receptor CD95 / Fas. The CD178-mediated apoptosis pathway has been implicated in peripheral tolerance, tissue pathology, and maintenance of the immune privileged sites. Defects in this interaction may be related to some cases of systemic lupus erythematosus (SLE). CD178 was also described as a co-stimulatory receptor for T-cell activation in mice in vivo.SpecificityThe antibody MEM-48 recognizes an extracellular epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (integrin beta2 subunit; beta2 integrin). CD18 is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.Application detailsFlow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
NOK-1
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD173 (blood group antigen H2) is a fucosylated saccharide (Fuc-alpha-1-2-Gal-beta-1-4-GlcNAc-beta) generated by beta-D-galactoside 2-alpha-L-fucosyltransferase (FUT1). CD173 belongs to markers of early hematopoiesis; it is expressed mainly on CD34-positive hematopoietic progenitor cells. CD173 is a precursor structure of CD174 (Lewis Y) and is also structurally related to CD15 (Lewis X). On endothelial cells CD173 and CD174 are concentrated on pseudopodial extensions responsible for initial contacts between endothelial cells.SpecificityThe mouse monoclonal antibody NOK-1 recognizes an extracellular epitope of CD178 / Fas-L, an approximately 40 kDa transmembrane glycoprotein expressed on neutrophils, monocytes, and activated T and NK cells.Application detailsFlow cytometry: Recommended dilution: 2-5 ?g/ml.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
MEM-195
Concentration:
1 mg/ml
Format:
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Storage buffer:
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
CD172g is a transmembrane glycoprotein, which may play a role in inter-T cellular signaling by binding CD47, and thus in influencing T cell behaviour. CD172g is expressed on mature thymocytes, CD4+ T cells, CD8+ T cells, NK cells, and some B cells. It is absent on myeloid cells. Engagement of CD172g by CD47 expressed on antigen presenting cells results in enhanced antigen-specific T cell proliferation and costimulates T cell activation.SpecificityThe antibody MEM-195 reacts with CD173 (H2), an extracellular saccharide antigen expressed mainly during early hematopoiesis; it is also expressed on endothelial cells.Application detailsFlow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
OX-119
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD172b, the signal-regulatory protein beta (SIRP beta) is a disulfide-linked homodimer expressed on myeloid cells including monocytes and dendritic cells. Similarly to CD172a, it serves as a negative regulator of tyrosine kinase-coupled signaling processes. Unlike CD172a, the CD172b protein does not possess the cytoplasmic domain, but instead its transmembrane domain can interact with another transmembrane protein DAP-12, which contains ITAM sequences in its intracellular domain and links CD172b to the downstream signaling molecules. The result is e.g. regulation of neutrophil transepithelial migration.SpecificityThe mouse monoclonal antibody OX-119 recognizes an extracellular epitope on CD172g, an approximately 55 kDa transmembrane glycoprotein expressed on most T cells, as well as on NK cells and some B cell populations.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
B4B6
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD172a, the signal-regulatory protein alpha (SIRP alpha), also known as SH2 domain-containing phosphatase substrate-1 (SHPS1), is a 75-110 kDa transmembrane glycoprotein expressed mainly on granulocytes, monocytes, macrophages, dendritic cells and neurons. Its extracellular ligand is CD47. CD172a serves as a substrate of activated receptor tyrosine kinases and upon phosphorylation it recruits SH2 domain-containing tyrosine phosphatases, thereby regulating signal transduction processes related to cell activation, transmigration and phagocytosis. CD172a is a specific marker of cardiomyocytes derived from human pluripotent stem cells and serves as a negative regulator of signaling and growth in myeloid progenitor cells. Extracellular part of CD172b is 90% identical to that of CD172a, but unlike CD172, it has dramatically reduced intracellular domain.SpecificityThe mouse monoclonal antibody B4B6 recognizes an extracellular epitope of CD172b, an approximately 50 kDa transmembrane glycoprotein expressed on myeloid cells.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
SE5A5
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD172a, the signal-regulatory protein alpha (SIRP alpha), also known as SH2 domain-containing phosphatase substrate-1 (SHPS1), is a 75-110 kDa transmembrane glycoprotein expressed mainly on granulocytes, monocytes, macrophages, dendritic cells and neurons. Its extracellular ligand is CD47. CD172a serves as a substrate of activated receptor tyrosine kinases and upon phosphorylation it recruits SH2 domain-containing tyrosine phosphatases, thereby regulating signal transduction processes related to cell activation, transmigration and phagocytosis. CD172a is a specific marker of cardiomyocytes derived from human pluripotent stem cells and serves as a negative regulator of signaling and growth in myeloid progenitor cells.SpecificityThe mouse monoclonal antibody SE5A5 recognizes a common extracellular epitope on human CD172a and CD172b antigens (approx. 90 kDa and approx. 50 kDa, respectively), although its reactivity with CD172a is higher.Application detailsFlow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
15-414
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD170, also known as Siglec 5 (sialic acid binding Ig-like lectin 5) is a type 1 transmembrane glycoprotein containing two cytoplasmic immunoreceptor tyrosine inhibitory motifs (ITIMs). CD170 forms homodimers and functions as an inhibitory receptor able to downregulate cell activation. It binds to alpha2,3- and alpha2,6-linked sialic acid ligands, e.g. on glycophorin A (CD235a). Aberrant expression of CD170 by CD34+ progenitor cells can be observed in case of acute myeloid leukemias.SpecificityThe mouse monoclonal antibody 15-414 recognizes en extracellular epitope of CD172a (SIRP alpha), an approximately 90 kDa transmembrane glycoprotein expressed on cells of myeloid origin and neurons.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml. <br>Immunohistochemistry: Recommended dilution: 5-10 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
1A5
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD169, also known as Siglec-1 or sialoadhesin, is a type I transmembrane glycoprotein of the sialic acid binding Ig-like lectin family. It binds to sialylated glycoproteins on various haematopoietic cells to mediate cell-cell interactions. CD169 is expressed on a subset of macrophages and dendritic cells. On CD14+ monocytes its expression can be induced by interferon alpha and gamma. High expression of CD169 is observed in the spleen, lymph nodes, bone marrow, and under inflammatory conditions rheumatoid arthritis and atherosclerosis, lower in the liver, lungs and gut. It has been shown to be involved in antigen presentation to invariant NKT cells, which play an important role in the innate arm of the immune system to modulate the subsequent acquired immune responses.SpecificityThe mouse monoclonal antibody 1A5 recognizes an extracellular epitope of CD170 (Siglec-5, sialic acid binding Ig-like lectin 5), a transmembrane glycoprotein expressed strongly by neutrophils, macrophages activated during infections, monocytes, and dendritic cells. As in case with other anti-CD170 antibodies, this antibody crossreacts with Siglec-14, whose first two Ig domains are almost identical to those of CD170.Application detailsFlow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
7-239
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD167a, also known as e.g. discoidin domain receptor tyrosine kinase 1 (DDR1), tyrosine kinase receptor E (TRKE), cell adhesion kinase (CAK), or neuroepithelial tyrosine kinase 4 (NEP, NTRK4), is a transmembrane receptor tyrosine kinase expressed predominantly in epithelial cells. It has been shown to be significantly overexpressed in several human tumors. Alternatively spliced transcript variants encoding different isoforms of this protein have been described. After binding to fibrilar collagens I, II, III, V, or basement membrane collagens IV and VIII, CD167a becomes activated and autophosphorylated and transduces collagen-induced signaling.SpecificityThe mouse monoclonal antibody 7-239 recognizes an extracellular epitope of CD169 (sialoadhesin, Siglec-1), a 210 kDa type I transmembrane glycoprotein expressed on macrophages and dendritic cells.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
51D6
Concentration:
1 mg/ml
Format:
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Storage buffer:
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
CD165 is a poorly characterized transmembrane protein highly expressed on platelets and many leukemic T cell lines. At lower level it is expressed on a proportion of circulating T cells and monocytes, on thymic epithelium, fibroblasts, epidermal keratinocytes, pancreatic islet cells, and some neurons. It might have a role in adhesion between thymocytes and thymic epithelial cells and it can be used as a marker for tumor progression.SpecificityThe mouse monoclonal antibody 51D6 recognizes an extracellular epitope of CD167a, an approximately 97-101 kDa receptor tyrosine kinase expressed mainly on epithelial cells, but also on B cells and dendritic cells.Application detailsImmunohistochemistry (frozen sections): Application note: acetone fixation. <br>Flow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
SN2
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD164, also known as endolyn, is a type I transmembrane protein with heavily glycosylated extracellular part containing sialic acid and glycosaminoglycan residues. CD164 plays both adhesive and antiadhesive role and serves as a potent negative regulator for CD34+ CD38- hematopoietic progenitor cell proliferation. It has also been reported to be involved in myogenic differentiation and cancer metastasis. The adhesive and negative regulatory functions seem to depend on different posttranslational modifications of CD164 protein.SpecificityThe mouse monoclonal antibody SN2, also known as SN2 N6-D11, recognizes an extracellular epitope of CD165, an approximately 37-42 kDa transmembrane glycoprotein expressed mainly on leukemic T cells, double positive and double negative thymocytes (CD4-CD8-, CD4+CD8+), and platelets.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
67D2
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD163, also known as M130, is a member of the scavenger receptor family, accounting for the clearance of hemoglobin-haptoglobin complexes during limited hemolysis, which protects the body, in particular the kidneys, against heme-mediated oxidative damages. It does not have measurable affinity for noncomplexed hemoglobin or haptoglobin. Immunomodulatory role of CD163 has been postulated. CD163 is expressed by cells of the monocyte-macrophage lineage and its extracellular part also circulates in plasma as a soluble protein, especially during sepsis and other conditions affecting macrophage activity, when its level may raise manyfold.SpecificityThe mouse monoclonal antibody 67D2 recognizes an extracellular class III epitope (not sensitive to sialidase, N-glycanase, O-glycosidase, and O-sialoglycoprotease) of CD164, a sialomucin expressed in hematopoietic myeloid and erythroid progenitors, activated basophils, and in various carcinomas and leukemic cells.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
GHI/61
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD162 (P-selectin glycoprotein ligand-1, PSGL-1) is a sialomucin constitutively expressed as a disulfide-linked homodimer of two 120 kDa subunits on the surface of circulating leukocytes. CD162 serves as a ligand for P- E- and L-selectin, with the highest affinity for P-selectin. It is thus involved in leukocyte rolling at the endothelial surfaces, prerequisite for firm leukocyte adhesion and subsequent transendothelial migration. CD162 also mediates leukocyte-platelet adhesion and interleukocyte contacts. Whereas serving as an adhession molecule on mature leukocytes, CD162 is a potent negative regulator of human hematopoietic progenitors.SpecificityThe mouse monoclonal antibody GHI/61 recognizes an extracellular epitope CD163, an approximately 130 kDa high affinity scavenger receptor expressed mainly on monocytes and macrophages, which binds hemoglobin-haptoglobin complex.Application detailsFlow cytometry: Recommended dilution: 1.5 - 2.5 ?g/ml; positive control: human peripheral blood.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
TC2
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD161, also known as Nkrp1 (natural killer receptor protein 1) or Klrb1 (killer cell lectin-like receptor subfamily b member 1), is a disulphide-linked homodimeric receptor, which is involved in regulation of NK cell and NKT cell function. It is expressed on rat NK cells, subset of T cells, dendritic cells, and activated monocytes. Although human CD161 is expressed as one isoform, the rat CD161 has three isoforms, referred to as CD161a, b, and c. These proteins contain C-terminal C-type lectin extracellular domain, a transmembrane domain, and N-terminal intracellular domain, which contains ITIM motif, such as CD161b, and displays inhibitory function, or does not contain ITIM motif, thus also not the inhibitory function, such as CD161a.SpecificityThe antibody TC2 reacts with an extracellular epitope of CD162, a 220 kDa type I integral membrane protein expressed as disulfide-linked homodimer (sialomucin family). CD162 is present on the most peripheral blood T lymphocytes, monocytes, granulocytes; it is also expressed on a subpopulation of B lymphocytes and CD34<sup>+</sup> bone marrow cells.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
HP-3G10
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD160 is a cell surface glycoprotein of immunoglobulin superfamily, which functions as a costimulatory receptor expressed mainly on cytotoxic cell populations and recognizing both classical and non-classical MHC class I molecules. It can form disulfide-linked multimers. Down-modulation of CD160 occurs as a consequence of its proteolytic cleavage and the released soluble form was found to impair the MHC-class I specific cytotoxicity of CD8+ T lymphocytes and NK cells. In contrast to GPI-anchored isoform with broader expression among CD160 positive cells, expression of the transmembrane isoform is restricted to NK cells and is activation-dependent.SpecificityThe mouse monoclonal antibody HP-3G10 recognizes an extracellular epitope of CD161, a type II transmembrane C-type lectin receptor, expressed on the plasma membrane of NK cells, dendritic cells, activated monocytes and a subset of T cells as a disulphide-linked homodimer.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgM kappa
Monosan Range:
MONOSAN
Clone:
BY55
Concentration:
1 mg/ml
Format:
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Storage buffer:
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms – FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.SpecificityThe mouse monoclonal antibody BY55 recognizes an extracellular epitope of CD160, a 27 kDa glycoprotein expressed on NK cells, NK-T cells, intestinal intraepithelial lymphocytes, TCR-gamma/delta T cells and a small population of TCR-alpha/beta T cells. The antibody detects both GPI-anchored and transmembrane form of CD160.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
MEM-168
Concentration:
1 mg/ml
Format:
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Storage buffer:
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
CD158f, also known as KIR2DL5, is a polymorphic 60 kDa transmembrane glycoprotein with two Ig-like extracellular domains by which it recognize HLA class I molecules. Its long intracellular domain contains immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that upon extracellular ligand-mediated phosphorylation serve as docking sites for inhibitory phosphatases, which results in blocking natural cytotoxicity as well as antibody-dependent cytotoxicity of the particular NK cell, and its adhesion toward target cells. Together with other killer inhibitory receptors CD158f is important for immunological tolerance to discriminate between normal and abnormal cells. Besides NK cells it is expressed on a small population of cytotoxic T cells. Expression of CD158f alleles is highly variable in the population.SpecificityThe antibody MEM-168 reacts with an extracellular epitope of CD16 antigen, a low affinity receptor for aggregated IgG (FcgammaRIII antigen). CD16 exists in two different isoforms: CD16a (FcgammaRIIIA; 50-65 kDa; expressed on NK-cells, monocytes and macrophages) and CD16b (FcgammaRIIIB; 48 kDa; mainly expressed on neutrophils).Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
UP-R1
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD158d / KIR2DL4 is a KIR family member that shares structural features with both activating and inhibitory receptors and may mediate different functions under different circumstances. It contains cytoplasmic ITIM, suggesting inhibitory function, but also transmembrane domain similar to those of activating KIRs. It has been reported that CD158d serves as an inhibitory receptor for peripheral and uterine NK cells, but its ligation with soluble mAbs (unlike immobilized mAbs) results in activation of IFN-γ secretion. CD158d also binds both membrane form and soluble form of its ligand HLA-G.SpecificityThe mouse monoclonal antibody UP-R1 recognizes an extracellular epitope on CD158f (KIR2DL5), a 60 kDa glycoprotein serving as a HLA class I ligand, and mainly expressed on a subset of NK cells and a small population of T cells. Its expression is highly polymorphic between individuals.Application detailsFlow cytometry: Recommended dilution: 2-6 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
mAb#33
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Killer cell immunoglobulin-like receptors (KIRs) are polymorphic transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. They are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain (such as CD158a / KIR2DL1) transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain (such as CD158g / KIR2DS5, CD158h / KIR2DS1, or KIR2DS3) lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for CD158 isoforms are subsets of MHC class I molecules.SpecificityThe mouse monoclonal antibody mAb#33 (also known as mAb 33 or 33) recognizes extracellular portion of CD158d / KIR2DL4, a 45 kDa NK cell marker. Cell surface expression and function of CD158d / KIR2DL4 depends on genotype of particular individuals.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG2b kappa
Monosan Range:
MONOSAN
Clone:
HP-MA4
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD157 (cADPr hydrolase 2) is a GPI-anchored ectoenzyme possessing ADP-ribosyl cyclase and cyclic ADP-ribose hydrolase activity. It uses NAD and cADP-ribose as substrates. CD157 is expressed on granulocytes, monocytes, macrophages, follicular dendritic cells, bone marrow stromal cells and human umbilical cord vein endothelial cells. In case of rheumatoid arthritis is expression is often higher and it is also differentially expressed in the myeloid leukemias. It may also have a signaling role.SpecificityThe mouse monoclonal antibody HP-MA4 recognizes an extracellular epitope of CD158 isoforms KIR2DL1 (CD158a), KIR2DS5 (CD158g), KIR2DS1 (CD158h), and KIRDS3. It does not recognize the isoforms CD158b1,d,f,i,j.Application detailsWestern blotting: Non-reducing conditions. <br>Immunohistochemistry: Recommended dilution: 5-10 ?g/ml. <br>Flow cytometry: Recommended dilution: 1-12 µg/ml
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
SY11B5
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD154 / CD40L (CD40 ligand) is a member of the tumor necrosis factor family, and is expressed primarily on activated CD4+ lymphocytes, but also on mast cells, basophils, eosinophils and human dendritic cells. Its counter-receptor CD40 is expressed on antigen presenting cells, including dendritic cells, macrophages, and B cells, and also on fibroblasts. Triggering of CD40 by CD40L causes maturation of dendritic cells and upregulation of antigen presentation in functions of the MHC and costimulatory molecules. CD40L also functions as a direct stimulating factor for T cells. CD40L plays also roles e.g. in antibody class switching and modulation of apoptosis in the germinal center.SpecificityThe mouse monoclonal antibody SY11B5 recognizes CD157, an approximately 45 kDa GPI-anchored extracellular protein expressed mainly on monocytes, macrophages, granulocytes and bone marrow stromal cells.Application detailsFlow cytometry: Recommended dilution: 2-6 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
24-31
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD152 / CTLA-4 is a homodimeric transmembrane protein similar to CD28 and binding the same ligands, i.e. CD80 (B7.1) and CD86 (B7.2), but with higher affinity. Unlike CD28 with important costimulating functions, CD152 acts as an important inhibitory receptor essential for modulation of the immune system. CD152 / CTLA-4 becomes transiently expressed on activated T cells and its malfunction can cause autoimmune diseases, such as insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, or thyroid-associated orbitopathy.SpecificityThe mouse monoclonal antibody 24-31 detects an extracellular epitope of CD154 / CD40L (CD40-ligand), a 39 kDa cell surface type II glycoprotein expressed predominantly on activated CD4+ lymphocytes.Application detailsFlow cytometry: Recommended dilution: 2-5 ?g/ml, Intracellular staining.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
BNI3
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD151, also known as PETA-3 (platelet-endothelial tetraspan antigen), is a four-pass transmembrane glycoprotein with short cytoplasmic N- and C-termini. CD151 is expressed mainly in platelets and megakaryocytes, immature hematopoietic cells, activated T cells, in endothelium, muscle cells, and epithelial cells. It associates with CD9, CD181, and integrin complexes alpha 3 / beta 1 (CD49c / CD29), alpha 5 / beta 1 (CD49e / CD29), and alpha 6 / beta 4 (CD49f / CD104). CD151 appears to be involved in cell adhesion and migration, including metastasis.SpecificityThe mouse monoclonal antibody BNI3 recognizes an extracellular domain of human CD152 / CTLA4, an approximately 45 kDa type I transmembrane protein serving as a negative regulator of T cell responses.Application detailsFlow cytometry: Recommended dilution: 2-4 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
50-6
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD15 (Lewis x), also known as stage specific embryonic antigen-1 (SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several structural relatives of Lewis x, e.g. sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease.SpecificityThe mouse monoclonal antibody CD151 recognizes an extracellular epitope of CD151 (also known as PETA-3), a 29 kDa transmembrane protein of tetraspanin family, expressed in many cell types.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgM kappa
Monosan Range:
MONOSAN
Clone:
MMA
Concentration:
1 mg/ml
Format:
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Storage buffer:
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
CD148 (also known as HPTP-eta or DEP-1) is a transmembrane protein tyrosin phosphatase, containing eight fibronectin type III extracellular domains. This protein is known to inhibit transduction of mitogenic signals in non-hematopoietic cells (fibroblasts, epithelial cells), and signal transduction downstream of T cell receptor, however, it also augments immunoreceptor signaling in B cells and macrophages via dephosphorylating C-terminal tyrosine of Src-family tyrosine kinases. CD148 expression increases after in vitro activation of peripheral blood leucocytes. It can be also used as marker of the most mature human thymocytes, and leukemic cells corresponding to this stadium of thymocyte differentiation. In contrast, in mice the CD148 expression sharply drops through the double positive stage to the single positive thymocytes.SpecificityThe mouse monoclonal antibody MMA reacts with an extracellular epitope of CD15, a cell membrane 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on granulocytes, monocytes, macrophages, mast cells; it is also present on Langerhans cells and some myeloid precursors cells. This antibody is a superior reagent for identifying of Hodgkin´s lymphoma.Application detailsFlow cytometry: Recommended dilution: 1-5 ?g/ml.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
MEM-CD148/05
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
A soluble recombinant form of CD147, CD147Rg, which consists of the cDNA coding for the entire extracellular region of CD147 fused to the DNA coding for the hinge region, CH2 and CH3 domain of human IgG1.
CD147 (basigin, neurothelin, OX-47, 5A11, CE9, M6) also known as EMMPRIN (extracellular matrix metalloproteinase inducer) or TCSF (tumour cell-derived collagenase-stimulatory factor) is an ubiquitously expressed cell surface protein with multiple glycosylated forms. The highest level of CD147 expression is on metabolically active cells, such as lymphoblasts, inflammatory cells, brown adipocytes and malignant tumour cells. CD147 has multiple functions, including facilitating of cell surface expression of monocarboxylate transporter proteins and extracellular matrix metalloproteinases, regulation of integrin functions, it plays roles in cell development and activation, fetal development or retinal function.SpecificityThe mouse monoclonal antibody MEM-CD148/05 recognizes an extracellular epitope of CD148, a highly glycosylated up to 250 kDa receptor-like protein tyrosin phosphatase expressed mainly in lymphocytes, myeloid cells and epithelial cells.Application detailsFlow cytometry: Recommended dilution: 4 ?g/ml. <br>Immunohistochemistry (paraffin sections): Recommended dilution: 10 ?g/ml; positive tissue: testis.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
MEM-M6/2
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD146, also known as MCAM (melanoma cell adhesion molecule) or MUC18, is a heavily glycosylated transmembrane glycoprotein with more than 50% of the mass from carbohydrates. It is expressed on epithelial and endothelial cells, fibroblasts, multipotent mesenchymal stromal cells, activated T cells and activated keratinocytes, and on some cancer cells, especially melanoma. The presence of CD146 on circulating blood cells has been confined to the activated T cells rather than circulating endothelial cells. CD146 mediates heterophilic cell adhesion and regulates monocyte transendothelial migration.SpecificityThe antibody MEM-M6/2 recognizes extracellular Ig domain D1 of CD147 (Neurothelin), a 50-60 kDa type I transmembrane glycoprotein primarily expressed on all leukocytes, red blood cells, platelets and endothelial cells; it is not expressed by resting lymphocytes.
The antibody MEM-M6/1 is a high-affinity antibody capable of binding to unstimulated peripheral blood T cells.Application detailsWestern blotting: Nonreducing conditions. <br>Flow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
P1H12
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD144 / VE-cadherin (cadherin 5) is the major cadherin that is present at endothelial junctions. It is also strictly endothelial specific. Under vascular permeability increasing conditions (and also in capillaries and veins) CD144 is being phosphorylated, which promotes its rapid and reversible internalization. On the contrary, binding of p120 catenin (delta1 catenin) maintains CD144 localization at the plasma membrane, which stabilizes the junction and reduces vascular permeability.SpecificityThe mouse monoclonal antibody P1H12 recognizes an extracellular epitope of CD146, a 118 kDa transmembrane glycoprotein expressed on epithelial and endothelial cells, fibroblasts, multipotent mesenchymal stromal cells, melanoma cells, activated T cells and activated keratinocytes.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
55-7H1
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD143, also known as ACE (angiotensin-converting enzyme), carboxycathepsin, kininase II, peptidase P, or peptidyl dipeptidase 1, is a transmembrane zinc metallopeptidase catalyzing the conversion of angiotensin I into the physiologically active angiotensin II, which is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This enzyme plays a key role in the renin-angiotensin system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified, and two most abundant spliced variants encode the somatic form and the testicular form, that are equally active. CD143 is expressed mainly on endothelial cells, but it can be found also e.g. on activated macrophages and histiocytes.SpecificityThe mouse monoclonal antibody 55-7H1 recognizes a calcium-independent extracellular epitope on CD144 (VE-cadherin, cadherin 5), an adhesion molecule expressed on endothelial cells.Application detailsFlow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
5-369
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD142, also known as coagulation factor III, tissue thromboplastin, and tissue factor. It is a transmembrane glycoprotein, which enables cells to initiate the blood coagulation cascades, and functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. It is the only one factor in the coagulation pathway for which a congenital deficiency has not been described.SpecificityThe mouse monoclonal antibody 5-369 recognizes an extracellular epitope of CD143, a 171 kDa type I transmembrane glycoprotein with metallopeptidase activity, expressed mainly on endothelial cells.Application detailsFlow cytometry: Recommended dilution: 3-12 µg/ml.<br>Western blotting: Reducing conditions preferred.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
HTF-1
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD140b / PDGF-RB (platelet-derived growth factor receptor beta) is a cell surface receptor for members of platelet-derived growth factor family, whose intracellular part contains a tyrosine kinase domain. CD140b forms homodimers, or heterodimerizes with CD140a / PDGF-RA. Whereas CD140a can have both pro-proliferative or anti-proliferative effects, the CD140b induces in various cell types their proliferation and migration. CD140b has also developmental roles in the cardiovascular system and is preferentially expressed on some tumours such as medulloblastoma.SpecificityThe mouse monoclonal antibody HTF-1, also known as HTF1-7B8, recognizes an extracellular epitope of CD142 (tissue factor, coagulation factor III), a type I glycoprotein expressed on endothelial cells, monocytes, macrophages, and platelets upon induction by inflammatory mediators, and expressed constitutively by some tumors, the vasculature, placenta, kidney, and central nervous system.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
18A2
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD140a / PDGF-RA (platelet-derived growth factor receptor alpha) is a cell surface receptor for members of platelet-derived growth factor family, whose intracellular part contains a tyrosine kinase domain. CD140a forms homodimers, or heterodimerizes with CD140b / PDGF-RB. Whereas CD140b induces in different cell types their proliferation and migration, the role of CD140a is more controversial, with pro-proliferative or anti-proliferative effects. CD140a has early developmental functions, mediates mesodermal cell migration, and later acts in signaling associated in epithelial-mesenchymal interactions.SpecificityThe monoclonal antibody 18A2 recognizes an extracellular epitope of CD140b / PDGF-RB, the 180-190 kDa beta chain of platelet-derived growth factor receptor, which is widely expressed on a variety of mesenchymal-derived cells and plays pro-proliferative roles.Application detailsFlow cytometry: Recommended dilution: 2 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
16A1
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where it serves as a multifunctional lipopolysaccharide receptor. It is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.SpecificityThe mouse monoclonal antibody 16A1 recognizes an extracellular epitope of CD140a / PDGF-RA, the 170 kDa alpha chain of platelet-derived growth factor receptor, which is widely expressed on a variety of mesenchymal-derived cells and plays pro-proliferative or anti-proliferative roles in various tumours.Application detailsELISA: The antibody MEM-18 has been tested as the detection antibody in a sandwich ELISA for analysis of human CD14 in combination with antibody B-A8 (cat. no. 11-304-C100). <br>Flow cytometry: recommended dilution: 4 ?g/ml. <br>Western blotting: Non-reducing conditions.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-18
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD137, also known as TNFRSF9 or 4-1BB, is an inducible costimulatory molecule expressed mainly on activated T cells. Its ligand, known as 4-1BBL, is expressed on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells. CD137 signaling leads to maintaining the survival of activated T cells and CD8+ memory T cells, and clonal expansion of T cells, but also to suppressing myelopoiesis and dendritic cell development. Triggered CD137 induces a cytokine release profile regulating peripheral monocyte survival. Soluble forms of CD137 may provide negative control mechanism for some immune responses.SpecificityThe antibody MEM-18 reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked extracellular membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes; also expressed by most tissue macrophages. In human, the epitope recognized by MEM-18 is located between amino acids 57-64.Application detailsFlow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
4B4-1
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD135 / FLT3, also known as FLK2 or STK-1 is a receptor tyrosine kinase that plays important roles in hematopoiesis. After binding of Flt3 ligand (FL), CD135 homodimerizes and stimulates proliferation, differentiation and protects the cell from apoptosis. The loss of CD90 and gain of CD135 expression marks the loss of self-renewal in hematopoietic stem cell population. Detectable CD135 expression appears first at low levels on the surface of primitive multilineage progenitor cells and disappears during defined stages of B-cell development, but is upregulated and maintained during maturation of monocytes. CD135 is also expressed on thymocytes, dendritic cell progenitors and on mature dendritic cells, as well as on various malignant hematopoietic cells.SpecificityThe mouse monoclonal antibody 4B4-1 recognizes an extracellular conformational epitope on CD137, an approximately 40 kDa type I transmembrane protein of the TNFR family expressed mainly on activated T cells.Application detailsFlow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
BV10A4
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD134 (TNFRSF4, also known as OX40) is a type I transmembrane glycoprotein of TNF/NGF receptor family expressed on activated T cells, fibroblasts, and hematopoietic precursors. Binding to its ligand (OX40L, TNFSF4) on antigen presenting cells gives to the T cell costimulatory signal, and this interaction results also in B cell proliferation and influences T cell memory pool. CD134 is upregulated at sites of inflammation, especially in case of multiple sclerosis and psoriatic lesions.SpecificityThe mouse monoclonal antibody BV10A4 (BV10) reacts with an extracellular epitope of CD135 (FLT3, FLK2, STK-1), a 130-160 kDa type I transmembrane receptor tyrosine kinase that is involved in early steps of hematopoiesis.Application detailsFlow cytometry: Recommended dilution: 3-6 ?g/ml. <br>Immunohistochemistry: Recommended dilution: 5-10 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
Ber-ACT35
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD133 (prominin 1) is a 5-transmembrane glycoprotein with extracellular N- and intracellular C-terminus. CD133 function remains to be elucidated, but it can be used as a cancer stem cell marker. Its expression pattern in progenitor cells is similar to CD34, i.e. on hematopoietic stem cells in bone marrow, cord blood, neural stem cells, retinoblastoma, or endothelial precursor cells (not mature endothelial cells). It is being used for identification and isolation of hematopoietic stem cells, including isolation for stem cell transplantation. Expression of CD133 correlates with differentiation of human colon cancer cells.SpecificityThe mouse monoclonal antibody Ber-ACT35 (also known as ACT35) recognizes an extracellular epitope of CD134 (TNFRSF4, OX40), an approximately 50 kDa type I transmembrane glycoprotein expressed on activated T cells.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml. <br>Western blotting: Recommended dilution: 1-2 ?g/ml; non-reducing conditions preferred.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
W6B3C1
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD129 serves as the high affinity alpha subunit of IL-9 receptor. It associates with CD132, the common gamma chain shared by receptors of many different cytokines. CD129 is expressed at low levels by T and B cells, blood cell progenitors, eosinophils, mast cells, epithelial cells, muscle cells and neurons. Its signaling (through JAK/STAT pathways) results in proliferative and anti-apoptotic response, which is critical e.g. for intrathymic T cell development and survival of various cell types. The gene for CD129 is located at the pseudoautosomal regions of X and Y chromosomes and it may be related with the development of asthma.SpecificityThe mouse monoclonal antibody W6B3C1 recognizes the extracellular glycosylated epitope 1 on human CD133 (CD133/1), a 120 kDa glycoprotein of prominin family, expressed e.g. on progenitor cells. This antibody is important for identification of stem cells and tumor cells.Application detailsFlow cytometry: It is recommended to use bright fluorochromes or signal multiplying detection.
Antibody Isotype:
IgG2b kappa
Monosan Range:
MONOSAN
Clone:
AH9R7
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD123 is the alpha chain of interleukin 3 receptor (IL-3R alpha). This subunit heterodimerizes with the interleukin 3 receptor beta chain (CD131), which is shared with other receptors. CD123 interacts with IL-3 specifically, but with low affinity, and association with the beta subunit confers high affinity binding to the receptor heterodimer. Both chains are required for signaling, but receptor activation and signal transduction depend on IL-3 binding to CD123 as the initial step.SpecificityThe mouse monoclonal antibody AH9R7 recognizes an extracellular epitope of CD129 / IL-9R alpha, a 57 kDa type I transmembrane glycoprotein expressed at low levels by lymphocytes, blood cell progenitors, eosinophils, mast cells, epithelial cells, muscle cells and neurons.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
6H6
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD122 (IL-2/IL-15R beta) constitutes together with CD132 (common gamma chain) and with CD25 (IL-2/IL15R alpha) the intermediate (CD122+CD132) and the high affinity (CD122+CD132+CD25) IL-2 and IL-15 receptor complex. CD122 is expressed on NK cells and lymphocytes, but at low level, unless the cell is activated. The cytoplasmic part of CD122 binds to Src-family and Jak-family kinases. The biological effect of CD122 ligation depends on whether IL-2 or IL-15 is bound to the receptor complex.SpecificityThe mouse monoclonal antibody 6H6 recognizes an extracellular epitope of CD123 (interleukin 3 receptor alpha), a 60-70 kDa transmembrane protein expressed by myeloid precursors, megakaryocytes, macrophages, dendritic cells, mast cells, basophils, and some B cells. This antibody does not inhibit IL-3 binding to its receptor.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
TU27
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD120a / TNF R1, also known as TNFR55 or TNFRSF1A, is a 55 kDa receptor for tumor necrosis factor alpha and it is expressed in most tissues. By binding its trimeric ligand the CD120a protein forms trimers and the conformation change leads to dissociation of the inhibitory factor SODD from its intracellular death domain and in formation of signaling platform. CD120a can mediate apoptosis, and function as a regulator of inflammation. Germline mutations of the extracellular domains of this receptor were found to be associated with the autosomal dominant periodic fever syndrome. The impaired receptor clearance is thought to be a mechanism of the disease.SpecificityThe mouse monoclonal antibody TU27 recognizes an extracellular epitope of CD122 (IL-2R beta), a 70-75 kDa type I transmembrane glycoprotein constitutively expressed by NK cells and a T cell subset, and upregulated upon activation.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
H398
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD11c (p150, alphaX integrin subunit) forms complex with CD18 (beta2 integrin subunit) and is expressed mainly on tissue macrophages and dendritic cells. CD11c binds to complement fragment iC3b, fibrinogen, VCAM-1 and ICAM-2 or e.g. CD90. Like other beta2 integrins, CD11c/CD18 plays roles in cell migration and phagocytosis. Moreover, interaction of CD11c/CD18 with plasminogen regulates plasmin activities, and interaction with heparin counteracts binding of iC3b.SpecificityThe mouse monoclonal antibody H398 recognizes the extracellular domain of CD120a, a 55 kDa receptor for tumor necrosis factor. The antibody blocks biological activity of both natural and recombinant human TNF alpha and TNF beta.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
BU15
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.SpecificityThe antibody BU15 reacts with an extracellular epitope of CD11c (alphaX, p150), a 150 kDa integrin expressed mainly on dendritic cells and tissue macrophages.Application detailsFlow cytometry: Recommended dilution: 1 ?g/ml; positive control: peripheral blood lymphocytes. <br> Immunoprecipitation: Positive control: granulocytes, CD11b/CD18 transfectants. <br>Immunoprecipitation with the antibody MEM-174 was successfuly performed using immuno-affinity sorbents (minicolumns). The final immunosorbent should contain 1-5 mg of antibody/1 ml of sorbent gel (e.g. agarose beads). It is recommended to stimulate positive control material by LPS 1-2 days before to achieve better expression of target antigen.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
MEM-174
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD118 (LIFR alpha) is a type I transmembrane glycoprotein of the cytokine receptor family, which associates noncovalently with CD130 to form the functional high affinity LIF receptor, which also acts as an oncostatin M receptor. CD118 alone binds LIF with low affinity. A secreted form of CD118 results from alternative splicing, and may have inhibitory effect, as it also binds LIF, although with low affinity. CD118 is not expressed on lymphocytes, but it is widely expressed outside the immune system. Soluble CD118 level rises during pregnancy, in parallel with a drop in circulating LIF levels.SpecificityThe antibody MEM-174 recognizes an extracellular epitope of CD11b antigen (Mac-1 alpha), a 165-170 kDa type I transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. The CD11b mediates neutrophil and monocyte interactions with stimulated endothelium.Application detailsFlow cytometry: Recommended dilution: 2-5 ?g/ml.
Antibody Isotype:
IgG1 kappa
Monosan Range:
MONOSAN
Clone:
12D3
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells.
CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells.
CD13 (ANPEP) is a 150-170 kDa type II transmembrane zinc-binding ectopeptidase expressed on various cell types. CD13 is a metalloprotease that preferentially catalyzes removal of neutral amino acids from small peptides, thus activating or inactivating bioactive peptides. CD13 also has a role in extracellular matrix degradation, antigen processing and signal transduction, is important in inflammatory responses, regulates intercellular contact, cell motility and vascularization.
CD13 (ANPEP) is a 150-170 kDa type II transmembrane zinc-binding ectopeptidase expressed on various cell types. CD13 is a metalloprotease that preferentially catalyzes removal of neutral amino acids from small peptides, thus activating or inactivating bioactive peptides. CD13 also has a role in extracellular matrix degradation, antigen processing and signal transduction, is important in inflammatory responses, regulates intercellular contact, cell motility and vascularization.
CD15 (Lewis x), also known as stage specific embryonic antigen-1 (SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
MEM-158
Concentration:
1 mg/ml
Storage buffer:
Tris buffered saline (TBS) solution with 15 mM sodium azide
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-28
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Mouse anti Human CD64 antibody, clone 10.1 recognizes the human CD64 cell surface antigen, a ~75 kDa glycoprotein expressed by monocytes. The antigen acts as a high affinity receptor for human IgG, and is also known as FcRI.Mouse anti Human CD64 antibody, clone 10.1 blocks binding of immunoglobulin to FcRI.
Mouse anti Human CD51/CD61 antibody, clone 23C6 recognizes the intact complex formed between the CD51 and CD61 molecules (alpha V and beta 3 integrins). This complex binds vitronectin at the RGD sequence and can also bind fibrinogen, von Willebrand factor, thrombospondin, fibronectin, osteopontin and collagen. Mouse anti Human CD51/CD61 antibody, clone 23C6 reacts with osteoclasts, placenta, melanoma cell lines and weakly with platelets.
LN-1 reacts with CDw75, a neuraminidase sensitive cell surface sialoglycan which is present on the cell membrane and cytoplasm of germininal center B-cells and derived lymphomas. CDw75 has a function in cell adhesion and is the ligand for CD22. LN-1 reacts with RBC precursors in bone marrow, ductal and ciliated epithelial cells of kidney, breast, prostate, pancreas, lung, and with gioblastomas and astrocytomas, and also Reed Sternberg cells in Hodgkins disease.
Antibody Isotype:
IgM,kappa
Monosan Range:
MONOSAN
Clone:
LN-1
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Epstein AL et. al. J of Immunology 133: 1028-1036 (1984)
Mouse anti human CD47 antibody, clone BRIC126 recognises the human CD47 cell surface glycoprotein, a heavily N-glycosylated 47-52 kDa molecule. CD47 is expressed on all cells and tissues so far examined, although expression is reduced on erythrocytes of the rare Rh null phenotype.
Mouse anti Human CD34 antibody, clone QBEND/10 recognizes the human CD34 antigen, also known as Hematopoietic progenitor cell antigen CD34. Human CD34 is 385 amino acid polypeptide containing a 31 residue signal peptide, cleaved to yield the ~110kDa mature form of CD34, a sialomucin single pass transmembrane glycoprotein. CD34 is expressed by stem cells (Kaufman et al. 2001) and small vessel endothelium (Ramani et al. 1990)Human CD34 exists as two isoforms, the full length form described here and a truncated isoform lacking the carboxy-terminal of the intracellular domain and containing some alternative sequence in the remaining intracellular region. Antibody binding epitopes on human CD34 have been classified according to their resistance to enzymatic degradation and grouped together using this and competitive binding assays (Lanza et al. 1999). Mouse anti Human CD34 antibody, clone QBEND/10 has been classified as binding to the class II epitope, resistant to neuraminidase treatment but sensitive to both glycoprotease and chymopapain digestion. Mouse anti Human CD34 antibody, clone QBEND/10 binds to a different eoptope to Mouse anti Human CD34, clone 581 (MCA1578) which binds to the class III epitope resistant to all three enzymzatic treatments (Nishio et al. 1996 In Leukocyte Typing VI). Clone QBEND 10 is expected to bind to both isoforms of human CD34 as it's binding epitope has been mapped to the extracellular domain between amino acids 43 and 49 by peptide microarray analysis (Jones et al. 1996, in Leukocyte Typ
Mouse anti Human CD34 antibody, clone QBEND/10 recognizes the human CD34 antigen, also known as Hematopoietic progenitor cell antigen CD34. Human CD34 is 385 amino acid polypeptide containing a 31 residue signal peptide, cleaved to yield the ~110kDa mature form of CD34, a sialomucin single pass transmembrane glycoprotein. CD34 is expressed by stem cells (Kaufman et al. 2001) and small vessel endothelium (Ramani et al. 1990)Human CD34 exists as two isoforms, the full length form described here and a truncated isoform lacking the carboxy-terminal of the intracellular domain and containing some alternative sequence in the remaining intracellular region. Antibody binding epitopes on human CD34 have been classified according to their resistance to enzymatic degradation and grouped together using this and competitive binding assays (Lanza et al. 1999). Mouse anti Human CD34 antibody, clone QBEND/10 has been classified as binding to the class II epitope, resistant to neuraminidase treatment but sensitive to both glycoprotease and chymopapain digestion. Mouse anti Human CD34 antibody, clone QBEND/10 binds to a different eoptope to Mouse anti Human CD34, clone 581 (MCA1578) which binds to the class III epitope resistant to all three enzymzatic treatments (Nishio et al. 1996 In Leukocyte Typing VI). Clone QBEND 10 is expected to bind to both isoforms of human CD34 as it's binding epitope has been mapped to the extracellular domain between amino acids 43 and 49 by peptide microarray analysis (Jones et al. 1996, in Leukocyte Typ
Mouse anti Human CD34 antibody, clone QBEND/10 recognizes the human CD34 antigen, also known as Hematopoietic progenitor cell antigen CD34. Human CD34 is 385 amino acid polypeptide containing a 31 residue signal peptide, cleaved to yield the ~110kDa mature form of CD34, a sialomucin single pass transmembrane glycoprotein. CD34 is expressed by stem cells (Kaufman et al. 2001) and small vessel endothelium (Ramani et al. 1990)Human CD34 exists as two isoforms, the full length form described here and a truncated isoform lacking the carboxy-terminal of the intracellular domain and containing some alternative sequence in the remaining intracellular region. Antibody binding epitopes on human CD34 have been classified according to their resistance to enzymatic degradation and grouped together using this and competitive binding assays (Lanza et al. 1999). Mouse anti Human CD34 antibody, clone QBEND/10 has been classified as binding to the class II epitope, resistant to neuraminidase treatment but sensitive to both glycoprotease and chymopapain digestion. Mouse anti Human CD34 antibody, clone QBEND/10 binds to a different eoptope to Mouse anti Human CD34, clone 581 (MCA1578) which binds to the class III epitope resistant to all three enzymzatic treatments (Nishio et al. 1996 In Leukocyte Typing VI). Clone QBEND 10 is expected to bind to both isoforms of human CD34 as it's binding epitope has been mapped to the extracellular domain between amino acids 43 and 49 by peptide microarray analysis (Jones et al. 1996, in Leukocyte Typ
CD108 (Sema7A) is a GPI-anchored semaphorin family member, which enhances central and peripheral axonal growth and is required for proper axon track formation during ebryogenesis. CD108 also regulates osteoclast differentiation and pre-osteoblastic cell migration, and in immune system affects cell proliferation, chemotaxis and cytokine release. On erythrocytes CD108 defines the JMH (John-Milton-Hagen) human blood group. CD108 signalizes through its receptors – plexin C1 and beta1 integrins.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
MEM-150
Concentration:
1 mg/ml
Storage buffer:
Tris buffered saline (TBS) solution with 15 mM sodium azide
CD27 is a transmembrane 55 kDa protein of the nerve growth factor-receptor family, expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. This sCD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70.
CD27 is a transmembrane 55 kDa protein of the nerve growth factor-receptor family, expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. This sCD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
LT27
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
The monoclonal antibody MEC14.7 recognizes mouse CD34, a single-pass type I membrane glycophosphoprotein present on small vessel endothelial cells and hematopoietic progenitor cells. The apparent molecular mass of CD34 is heterogeneous, depending on the glycosylation state in different cell types. In cultured endothelioma cell lysate, CD34 has a molecular weight of ~100 kDa, whereas in lung lysates it is ~80 kDa. 2 Isoforms of CD34 exist, both are expressed on the cell surface.CD34 is an adhesion molecule performing a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. CD34 acts as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 presents carbohydrate ligands to selectins.CD34 is widely used as a marker to select early hematopoietic stem and progenitor cells in experimental and clinical hematopoiesis. The monoclonal antibody MEC14.7 recognizes a neuraminidase sensitive epitope on endothelium in vivo, particularly on small vessels and neoformed capillaries and developing vascular structures in embryonal structures. The monoclonal antibody MEC14.7 can be used for identification and characterization of capillary endothelial cells. Furthermore, the antibody is useful for isolation and characterization of hematopoietic progenitor cells, particularly of myelomonocytic colony forming cells. Monoclonal antibody MEC14.7 is also useful for immunopurification and cell separation
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
MEC14.7
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Garlanda et al. Eur J Cell Biol 1997;73:368
References 2:
Dong et al. Arterioscl Thromb Vac Biol 1997;17:1599
References 3:
Solberg et al. Development 2003;130;4439
References 4:
Almholt et al. Oncogene 2003;22:4389
References 5:
Sho et al. Arterioscl Thromb Vac Biol 2004;24:1916
CD99 (E2, MIC2) is a transmembrane glycoprotein that is involved in regulation of T cell addhesive properties and programmed cell death distinct from typical apoptosis course. CD99 roles are specific to certain stages of T cell differentiation such as corticothymocytes. CD99R isoform expression is restricted in the haematopoietic system to T, NK and myeloid cells.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
MEM-131
Concentration:
1 mg/ml
Storage buffer:
Tris buffered saline (TBS) solution with 15 mM sodium azide
CD59 (protectin) is a small (18-20 kDa) GPI-anchored ubiquitously expressed inhibitor of the membrane attack complex (MAC). It is thus the key regulator that preserves the autologous cells from terminal effector mechanism of the complement cascade. CD59 associates with C5b-8 complex and thereby counteracts appropriate formation of cytolytic pore within the plasma membrane. CD59 is also an low-affinity ligand of human CD2 and causes T cell costimulation.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
MEM-43/5
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD55 (decay-accelerating factor, DAF) is a GPI-anchored membrane glycoprotein that protects autologous cells from classical and alternative pathway of complement cascade. Bidirectional interactions between CD55 and CD97 are involved in T cell regulation and CD55 can still regulate complement when bound to CD97. In tumours, besides protection agains complement, CD55 promotes neoangiogenesis, tumorigenesis, invasiveness and evasion of apoptosis.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
MEM-118
Concentration:
1 mg/ml
Storage buffer:
Tris buffered saline (TBS) solution with 15 mM sodium azide
CD45RB is an of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RB is expressed e.g. in microglia and inflammatory cells.
CD45RB is an of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RB is expressed e.g. in microglia and inflammatory cells.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-55
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation; binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-83
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD44, also designated Pgp-1 or H-CAM, is a broadly distributed 85 kD protein. Amongst haematopoietic cells CD44 is expressed on B and T lymphocytes, monocytes and neutrophils. Other CD44-positive cell types include epithelial cells, glial cells, fibroblasts and myocytes. This broad distribution suggest a general role in cell-cell and cell-matrix adhesion. CD44 is involved in T cell activation and adhesion. Triggering of CD44 increases homotypic T cell aggregation mediated via LFA-1. CD44 is linked to the cytoskeleton. Furthermore CD44 has an accessory role in lymphocyte adhesion to high endothelial venules.
The monoclonal antibody MEC7.46 recognizes the mouse form of the platelet-endothelial cell adhesion molecule (PECAM)-1 (CD31). PECAM-1 is a member of the immunoglobulin superfamily. This heavily glycosylated protein is found in the entire vascular endothelium of adult mice and functions in mediating cellular adhesion by heterophilic and homophilic mechanisms. PECAM-1 is detected within the lymphopoietic islands in the spleen of newborn (day 12) and in the bone marrow of adult mice. Capillary endothelial cells of adult mice also express PECAM-1.<br /> The reactivity of the monoclonal antibody MEC7.46 is restricted to the isoform of the molecule that is selectively expressed by endothelial cells. The antibody precipitates a 130 kDa molecule present on the membrane of endothelial cells of all mouse blood vessels both in normal, inflamed and tumor tissues. The antigen is predominantly present at the lateral borders of endothelial cells as described for human PECAM-1. Staining of MEC7.46 can be seen on capillaries, veins, arteries and liver sinusoids.
P-selectin (Selectin P, GMP-140, LECAM3, CD62 antigen-like family member) is a 140 kDa type 1 transmembrane glycoprotein, and is one of the most commonly studied proteins that regulate cell rolling. P-selectin is stored in the Weibel-Palade bodies of endothelial cells, as well as in a-granules of platelets. From there, it can be rapidly brought to the cell surface after exposure to thrombin, histamine, complement 5a, Ca21 ionophores, or adenosine diphosphate.
CD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH. CD71 is also involved in uptake of non-transferrin bound iron.
CD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH. CD71 is also involved in uptake of non-transferrin bound iron.
CD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH. CD71 is also involved in uptake of non-transferrin bound iron.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-75
Concentration:
1 mg/ml
Storage buffer:
Tris buffered saline (TBS) solution with 15 mM sodium azide
CD59 (protectin) is a small (18-20 kDa) GPI-anchored ubiquitously expressed inhibitor of the membrane attack complex (MAC). It is thus the key regulator that preserves the autologous cells from terminal effector mechanism of the complement cascade. CD59 associates with C5b-8 complex and thereby counteracts appropriate formation of cytolytic pore within the plasma membrane. CD59 is also an low-affinity ligand of human CD2 and causes T cell costimulation.
CD59 (protectin) is a small (18-20 kDa) GPI-anchored ubiquitously expressed inhibitor of the membrane attack complex (MAC). It is thus the key regulator that preserves the autologous cells from terminal effector mechanism of the complement cascade. CD59 associates with C5b-8 complex and thereby counteracts appropriate formation of cytolytic pore within the plasma membrane. CD59 is also an low-affinity ligand of human CD2 and causes T cell costimulation.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
MEM-43
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils. CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein.
CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils. CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein.
CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils. CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein.
CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-112
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
MEM-111
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD53 is a tetraspanin family transmembrane glycoprotein expressed in the lymphoid-myeloid lineage. This molecule has been reported to form complexes with other leukocyte surface proteins such as CD2, CD19, CD21, MHC II, VLA-4 or tetraspanins CD37, CD81 and CD82, thus probably modulating various signaling processes. CD53 is involved in radioresistancy of tumour cells and its triggering has anti-apoptotic effect. In thymus, CD53 is up-regulated in response to positive selection signals during T cell development, and is strongly expressed upon macrophage exposure to bacterial lipopolysaccharide, whereas stimulation of neutrophils results in down-regulation of CD53 expression.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-53
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD45RA is a high molecular weight isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RA is expressed e.g. on naïve T cells and normal plasma cells.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
MEM-56
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD44 cell surface antigen is a 100 kDa type 1 transmembrane glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing.
CD44 cell surface antigen is a 100 kDa type 1 transmembrane glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing.
CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing, it can thus serve e.g. as a modulator of macrophage recruitment in response to pathogen. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
MEM-85
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negative charge, expressed on the surface of most hematopoietic cells. CD43 contributes to a repulsive barrier that interferes with cellular adhesion, however, in certain cases also promotes leukocyte aggregation. By interaction with actin-binding proteins ezrin and moesin CD43 plays a regulatory role in remodeling T-cell morphology and regulates cell-cell interactions during lymphocyte traffic. CD43 signaling both enhances LFA-1 adhesiveness and counteracts LFA-1 induction via other receptors. Expression of CD43 causes induction of functionally active tumour suppressor p53 protein, but in case of p53 and ARF defficiency CD43 promotes tumour proliferation and viability. It appears to be an important modulator of leukocyte functions.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-59
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD20 is a cell surface 33-37 (depending on the degree of phosphorylation) kDa non-glycosylated surface phosphoprotein expressed on mature and most malignant B cells, but not stem cells or plasma cells (low number of the CD20 has been also detected on a subpopulation of T lymphocytes and it can be expressed on follicular dendritic cells). Its expression on B cells is synchronous with the expression of surface IgM. CD20 regulates transmembrane calcium conductance (probably functioning as a component of store-operated calcium channel), cell cycle progression and B-cell proliferation. It is associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following mAb treatment.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-97
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD18 integrin beta 2 subunit is a 90 kDa type I transmembrane protein expressed on all leucocytes. CD18 can combine with integrin molecules CD11a-c to form heterodimers at the cell surface, and these heterodimers are known to participate in the process of cell adhesion as well as cell-surface mediated signaling. CD18 forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits, and these integrins are essential for proper leukocyte migration, mediating intercellular contacts.
Monosan Range:
MONOSAN
Clone:
CLB-LFA-1/1
Conjugate:
FITC
Concentration:
n/a
Storage buffer:
PBS with BSA and 0.1% sodium azide
Storage:
2-8°C
References 1:
Hajishengallis G et al. Clin.Diagn Lab.Immunol 2002
CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where it serves as a multifunctional lipopolysaccharide receptor. It is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-18
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD17, lactosylceramide, is an ubiquitous glycosphingolipid with uncharged disaccharide headgroup, highly enriched in lipid raft-derived structures. Besides playing a pivotal role in the biosynthesis of complex glycosphingilipids, lactosylceramide is involved in cell-cell and cell-matrix interactions and in signaling events linked to cell differentiation, development, apoptosis and oncogenesis. Lactosylceramide regulates integrin functions and production of nitric oxide. Its expression defines successive stages in the maturation of myeloid cells.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
MEM-68
Concentration:
1 mg/ml
Storage buffer:
Tris buffered saline (TBS) solution with 15 mM sodium azide
CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation; binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-25
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD10 (neutral endopeptidase – NEP, common acute lymphocytic leukemia antigen – CALLA, membrane metallo-endopeptidase – MME, enkefalinase) is a 100-kDa cell surface zinc metalloprotease, cleaving peptide bonds on the N-terminus of hydrophobic amino acids and inactivating multiple physiologically active peptids. CD10 is expressed on various normal cell types, including lymphoid precursor cells, germinal center B lymhocytes, and some epithelial cells, and its expression level serves as a marker for diagnostics of many carcinomas. CD10 is also a differentiation antigen for early B-lymphoid progenitors in the B-cell differentiation pathway and has a key role in regulation of growth, differentiation and signal transduction of many cellular systems.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-78
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
The CD8 T cell coreceptor (monomer approx. 32-34 kDa) is expressed as alpha/beta heterodimer on majority of MHC I-restricted conventional T cells and thymocytes and as alpha/alpha homodimer on subsets of memory T cells, intraepithelial lymphocytes, NK cells and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha-beta but not alpha-alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
The CD8 T cell coreceptor (monomer approx. 32-34 kDa) is expressed as alpha/beta heterodimer on majority of MHC I-restricted conventional T cells and thymocytes and as alpha/alpha homodimer on subsets of memory T cells, intraepithelial lymphocytes, NK cells and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha-beta but not alpha-alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
MEM-31
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
MEM-115
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD48 (Blast-1) belongs to the CD2 subset of the Ig superfamily, which includes CD2, CD2F-10, CD58, CD84, CD150, CD229, CD244 and others. These molecules bind to the same or another members of their family, thus mediate homotypic or heterotypic adhesion. CD48 is a GPI-anchored protein broadly expressed on hematopoietic cells and serves as a high affinity ligand for 2B4 and low affinity ligand for CD2. 2B4-CD48 interaction among NK cells and NK-T cells regulates cell proliferation. Signaling through CD48 results in eosinophil activation and CD48 expression is increased in several infectious diseases.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-102
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
The antibody HL-37 reacts with polymorphic determinant on human HLA-DQ1 and HLA-DQ3 molecules (recognized epitope was found on isolated beta chain of DQ1), but does not react with HLA-DQ2. DQ is the isotype of human MHC Class II molecules expressed on antigen-presenting cells (APC; dendritic cells, B lymphocytes, monocytes, macrophages).
Antibody Isotype:
IgG3
Monosan Range:
MONOSAN
Clone:
HL-37
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
HLA-DR, a member of MHC class II glycoproteins, that bind intracellularly processed peptides and present them to the Th cells, is composed of 36 kDa alpha chain and 27 kDa beta chain, both anchored in the plasma membrane. Together with other MHC II molecules HLA-DR plays a central role in the immune system.
Antibody Isotype:
IgG3
Monosan Range:
MONOSAN
Clone:
HL-39
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
The antibody reacts with human leukocyte antigen-DR (HLA-DR). The mAb has lymph node germinal center and mantle zone B cell reactivity. It reacts with interdigitating histiocytes in T cell zones and with sinus histiocytes and endothelial cells. It has also tumor specificity and reactivity with normal non-lymphoid tissue.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
LN-3
Concentration:
250 ug/ml
Storage buffer:
serumfree culture supernatant with 0.7% BSA and 0.1% sodium azide
CD28, a glycoprotein of 44-88 kDa, is the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. While 204-12 remained unclustered at the VIth International Workshop on human leukocyte differentiation antigens, the antibody has later been verified as CD28 specific.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
204-12
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Kishimoto T. et al., eds. Leukocyte Typing VI, Garland Publishing, (1997)
Moon-4 is specific for CD29, an adhesion receptor and costimulatory molecule. This antigen was initially characterized as gpIIa on platelets and as the common ?1 subunit of the very late antigen (VLA) protein family. CD29 forms a non-covalent heterodimeric complex with integrin ? subunits. It is also mediates adhesion to invasin and thrombospondin.
Antibody Isotype:
IgG1,kappa
Monosan Range:
MONOSAN
Clone:
Moon-4
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Deaglio, S. et. al, J Immunol 160(1): 395-402 (1998)
152-1D5 reacts with human CD84 (Mw 74 kDa). CD84 is expressed on mature B-cells and on Bcell lines but not on plasma cell lines. Immunohistochemistry demonstrated that it strongly stains tissue macrophages. It is also expressed on platelets and at low levels on blood T-cells. It is a highly N-glycosylated protein and belongs to immunoglobulin superfamily. It may play a role in leukocyte activation but cellular expression does not significantly increase after activation. 152- 1D5 was clustered at the Vth WLDA.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
152-1D5
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Schlossman SF et al. (eds) Leukocyte Typing V. p.699-700, (1995)
References 2:
De la Fuente, M, et al, Blood 90(6): 2398-2405 (1997)
1.3.3.22 Reacts with human CD81 (TAPA-1), a 24 kDa member of the TM4 superfamily of proteins with four transmembrane domains. MAbs to CD81 have been shown to have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas. CD81 can associate with CD37 and/or CD53, or on the surface
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
1.3.3.22
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Kishimoto T. et al., eds. Leukocyte Typing VI, Garland Publishing, (1997)
CD29 (beta1 integrin subunit, GPIIa) forms non-covalently linked heterodimers with at least 6 different alpha chains (alpha1-alpha6, CD49a-f) determining the binding properties of beta1 (VLA) integrins. These integrins mediate cell adhesion to collagen, fibronectin, laminin and other extracellular matrix (ECM) components. This interaction hinders cell death, whereas disruption of anchorage to ECM leads to apoptosis. Decreased expression of most beta1 integrins correlates with acquiring multidrug resistance of tumour cells during selection in presence of antitumour drug. In platelets, translocation of intracellular pool of beta1 integrins to the plasma membrane following thrombin stimulation. These integrins are also up-regulated in leukocytes during emigration and extravascular migration and appear to be critically involved in regulating the immune cell trafficking from blood to tissue, as well as in regulating tissue damage and disease symptoms related to inflammatory bowel disease. Through a beta1 integrin-dependent mechanism, fibronectin and type I collagen enhance cytokine secretion of human airway smooth muscle in response to IL-1beta.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-101A
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CDw78 (also called Ba antigen, Leu21 or LO panB a) is present on some immature and some mature B-cells. The antigen appears on B-cell progenitors preceding CD10, CD19, CD22, and CD37. It is expressed on resting B-cells and reappears and persists in the cytoplasm and on the cell surface until cytoplasmic Ig appears. Its expression is greatly increased after B-cell activation in vitro. It is also found on tissue macrophages and on epithelial cells, but not on T-cells, NK cells, monocytes, granulocytes, thymocytes or bone marrow stromal fibroblasts nor myeloid tissues. 60-3G2 was typed at CD workshop IV.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
60-3G2
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Pinchouk VG. et al, Anticancer Research 8: 1377-1380 (1988)
References 2:
Gluzman DF. et al, Tissue Antigens 33: 151 (1989)
References 3:
Sidorenko SP. et al, Neoplasma 39: 3-9 (1992)
References 4:
Moldenhauer et al, Leucocyte Typing IV, pp 155 162, (1989)
References 5:
Pezzuto et al, Leucocyte Typing IV, pp 165 174, (1989)
CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where it serves as a multifunctional lipopolysaccharide receptor. It is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-15
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Protein A-CR purified soluble recombinant form of CD147, CD147Rg, which consists of the cDNA coding for the hinge region, CH2-and CH3 domain of human IgG1 (CD147Rg is secreted by transfectants as a dimer).
CD147 (basigin, neurothelin, OX-47, 5A11, CE9, M6) also known as EMMPRIN (extracellular matrix metalloproteinase inducer) or TCSF (tumour cell-derived collagenase-stimulatory factor) is an ubiquitously expressed cell surface protein with multiple glycosylated forms. The highest level of CD147 expression is on metabolically active cells, such as lymphoblasts, inflammatory cells, brown adipocytes and malignant tumour cells. CD147 has multiple functions, including facilitating of cell surface expression of monocarboxylate transporter proteins and extracellular matrix metalloproteinases, regulation of integrin functions, it plays roles in cell development and activation, fetal development or retinal function.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-M6/1
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD98 (4F2) is a type II transmembrane glycoprotein which serves as the heavy chain of the heterodimeric amino acid transporters (HATs). CD98, linked to various light chains by disulfide bond, is responsible for cell surface expression and basolateral localization of this transporter complex in polarized epithelial cells and also interacts with beta1 integrins and increases their affinity for ligand. Besides its roles in amino acid transport, CD98 is thus involved in cell fusion and activation. It is implicated in regulation of cellular differentiation, growth and apoptosis.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-108
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD50 (intracellular adhesion molecule 3, ICAM-3) is a transmembrane glycoprotein expressed by leukocytes, that serves as a counter-receptor for the lymphocyte function-associated antigen (LFA)-1 integrin. Besides functioning as an adhesive molecule that mediates e.g. the contact between T cells and antigen presenting cells, ICAM-3 regulates affinity of LFA-1 for ICAM-1 and induces T cell activation and proliferation. ICAM-3 plays an essential role in the initiation of the immune response both on T cells and antigen presenting cells and interacts also with CD209 (dendritic cell-specific ICAM-3-grabbing nonintegrin, DC-SIGN), a C-type lectin of dendritic cells and macrophages; this process is involved in dialogue between dendritic cells and granulocytes.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-171
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD22, also known as Siglec-2 (sialic acid-binding immunoglobulin-like lectin-2) is a transmembrane glycoprotein binding alpha2,6-linked sialic acid-bearing ligands. Intracellular domain of CD22 recruits protein tyrosine phosphatase SHP-1 through the immunoreceptor tyrosine-based inhibitory motifs (ITIMs), thus setting a treshold for B cell receptor-mediated activation. CD22 also regulates B-cell response by involvement in controlling the CD19/CD21-Src-family protein tyrosine kinase amplification pathway and CD40 signaling. CD22 exhibits hallmarks of clathrin-mediated endocytic pathway.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
MEM-01
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD23 is a 45 kDa glycoprotein which is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts. The CD23 molecule is identical to the low affinity IgE receptor found on B cells. Expression of CD23 has been detected in neoplastic cells from cases of B cell chronic lymphocyctic leukaemia and some cases of centroblastic/centrocytic lymphoma. CD23 is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts.
CD22 (BL-CAM) is a type 1 integral membrane glycoprotein with molecular weight of 130 to 140 kDa. CD22 is expressed in both the cytoplasm and cell membrane of B-lymphocytes. CD22 antigen appears early in B-cell lymphocyte differentiation at approximately the same stage as the CD19 antigen. Unlike other B-cell markers, CD22 membrane expression is limited to the late differentiation stages comprised between mature B cells (CD22+) and plasma cells (CD22-), and may thus prove useful in phenotyping mature leukemias.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
RFB4
Conjugate:
R-PE
Concentration:
n/a
Storage buffer:
PBS with 4mg/ml BSA and 0.1% sodium azide
Storage:
2-8°C
References 1:
Bechanova V et al. Am Assoc for Cancer Res 2015
References 2:
Pantelyushin S et al. J of Int Soc for Anal Cytol. 2020
CD22 (BL-CAM) is a type 1 integral membrane glycoprotein with molecular weight of 130 to 140 kDa. CD22 is expressed in both the cytoplasm and cell membrane of B-lymphocytes. CD22 antigen appears early in B-cell lymphocyte differentiation at approximately the same stage as the CD19 antigen. Unlike other B-cell markers, CD22 membrane expression is limited to the late differentiation stages comprised between mature B cells (CD22+) and plasma cells (CD22-), and may thus prove useful in phenotyping mature leukemias.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
RFB4
Conjugate:
FITC
Concentration:
n/a
Storage buffer:
PBS with 4mg/ml BSA and 0.1% sodium azide
Storage:
2-8°C
References 1:
Bechanova V et al. Am Assoc for Cancer Res 2015
References 2:
Pantelyushin S et al. J of Int Soc for Anal Cytol. 2020
Mouse anti Human von Willebrand factor antibody, clone RFF-VIII R/1 recognizes human von Willebrand factor (vWF), also known as Factor VIII related antigen, a blood glycoprotein involved in blood coagulation. It stabilises circulating Factor VIII by binding to it and protecting it from cleavage and delivers it to sites of vascular injury. vWF also promotes the adhesion of platelets to sites of vascular damage by forming a molecular bridge between collagen on exposed endothelial cells and the GPIb binding sites of platelets circulating in the blood. vWF circulates in the blood as large multimers, with each monomer (250kDa) containing a number of specific domains. Mouse anti Human von Willebrand factor, clone RFF-VIII R/1 has a high affinity for an epitope within the platelet GPIb-binding site that is responsible for biological activity. As such the antibody is a potent inhibitor of vWF activity. It can completely neutralise ristocetin-induced platelet aggregation and ristocetin-induced binding of vWF to platelets. It also inhibits platelet adhesion to glass beads. The epitope recognized is present only on the intact multimeric form of vWF and is abolished by mild denaturation with SDS. Mouse anti Human von Willebrand factor, clone RFF-VIII R/1 does not recognize human Factor VIII.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
RFF-VIII R/1
Concentration:
IgG 1 mg/ml
Format:
Purified
Storage buffer:
PBS with <0.1% sodium azide
Storage:
aliquots -20°C. Thawed 2-8°C
References 1:
Goodall AH et al. Br J Haematol 1985; 59: 565-77
References 2:
Kraus E et al. Thromb Res 2014; 2134: 1285-91
References 3:
Chen YJ et al. Stroke 2015; 46: 237-44
References 4:
Tejada de Rink MM et al. Ther Hypothermia Tem Manag 2020; 10: 91-101
Mouse anti Human Troponin I (Cardiac) antibody, clone 10F4 (110) recognizes aa 34-37 of Troponin I from human heart. There is no cross-reactivity with skeletal muscle troponin I.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
10F4 (110)
Concentration:
IgG 1 mg/ml
Format:
Purified
Storage buffer:
PBS with <0.1% sodium azide
Storage:
aliquots -20°C. Thawed 2-8°C
References 1:
Marian AJ et al. Circ Res 1997; 81: 76-85
References 2:
Zhang J et al. Toxicol Pathol 2008; 36: 277-8
References 3:
Filatov VL et al. Biochem Mol Biol Int 1998; 45: 1179-87
Mouse anti Human Tenascin antibody, clone T2H5 recognizes tenascin in both normal and hyperproliferative (also neoplastic) tissues.Tenascin has been implicated in the guidance of migrating neurons and axons during development and neuronal regeneration. It promotes cortical neuron outgrowths and is an important component of the wound matrix and scar formation.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
T2H5
Concentration:
IgG 1 mg/ml
Format:
Purified
Storage buffer:
PBS with <0.1% sodium azide
Storage:
aliquots -20°C. Thawed 2-8°C
References 1:
Verstraeten AA et al. Br J Dermatol 1992; 127: 571-4
References 2:
Le Poole IC et al. Br J Dermatol 1997; 137: 171-8
References 3:
Milz S et al. J Anat 2007; 211: 1-7
References 4:
Nakamura Y et al. J Immunol 2004; 172: 1945-52
References 5:
Milz S et al. Invest Opthalmol Vis Sci 2002; 43: 1330-4
Mouse anti Human Talin-2 antibody, clone 53.8 (also designated 121A) recognizes Talin-2, a high molecular weight skeletal protein involved in focal adhesion plaques. It plays a role in linking integrin to the actin cytoskeleton. It interacts with phosphatidylinositol-4-phosphate 5-kinase type I gamma (PIP5K1C), inducing its kinase activity. Mouse anti Human Talin-2 antibody, clone 53.8 recognizes an epitope within the range of ammino acids 2470-2500.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
53.8
Concentration:
IgG 1 mg/ml
Format:
Purified
Storage buffer:
PBS with <0.1% sodium azide
Storage:
aliquots -20°C. Thawed 2-8°C
References 1:
Liu J et al. PNAS 2015; 112: E4864-73
References 2:
Prince HE & Yeh C. J Clin Lab Anal 2013; 27: 27-30
Mouse anti Human Talin-1 antibody, clone 97H6 recognizes an epitope within the region of amino acids 482-655 of humanTalin-1, a high molecular weight cytoskeletal protein, linking vinculin to the integrins, thereby linking the cytoskeleton to the extracellular matrix. It is specifically found in regions of cell-substratum contact and in lymphocytes at cell-cell contact points. Talin-1 plays a role in the assembly of actin filaments and in the migration of cells such as osteoclasts and fibroblasts
IPO-24 reacts with CD37, a B lineage restricted antigen, strongly expressed throughout B-cell ontogeny from pre B-cell until plasmablasts. Plasma cells are negative. IPO-24 reacts with human B-cell lines Daudi, Raji, Namalva, Ramos and CABIL, whereas T-cell lines CEM, Molt-4, Jurkat, MT-4, H9 and non-lymphoid cell lines MeWo and A-431 are negative. IPO-24 stains Bcell follicles and the most strongly labelled elements are mantle zone lymphoid cells. Neutrophils, macrophages, thymocytes and dendritic reticulum cells are negative.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
IPO-24
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
G. Moldenhauer G. et al., CD37 Workshop Panel report in: Schlossman et al., Leucocyte Typing V, B9, 544-545, (1995)
References 2:
Sidorenko S.P. et al., J. Tumor Marker Oncology 5: 232 (1990)
References 3:
Sidorenko S.P. et al., Haemat. Transfusiol. 35 N4: 19-22 (1990)
Mouse anti Human Synaptophysin antibody, clone SP15 recognizes human synaptophysin, also known as Major synaptic vesicle protein p38. Synaptophysin is a 313 amino acid synaptic vesicle associated protein of ~38kDa containing a single marvel domain. Synaptophysin expression is ubiquitous in synaptic vesicle membranes where it is the most abundant protein and is involved in the regulation of endocytosis (Kwon et al. 2011)
Mouse anti Human Protein Gene Product 9.5 antibody, clone 31A3 recognizes protein gene product 9.5 (PGP9.5), a ubiquitin hydrolase which is widely expressed in neuronal tissues and represents 1-2% of total soluble brain proteins. PGP9.5, also known as ubiquitin C-terminal hydrolase 1 (UCHL-1), is involved in the regulation of the ubuiquitin pathway. Mouse anti Human Protein Gene Product 9.5 antibody, clone 31A3 stains neuronal cell bodies and axons in the CNS and periphery, small nerve fibres in peripheral tissues, neuroendocrine cells in the pituitary, thyroid, pancreas and tumours of the DNES.
Mouse anti Human pepsinogen 1 antibody, clone 8003 recognizes human Pepsinogen I, a zymogen or proenzyme secreted by chief cells in the stomach. It is cleaved to form pepsin both in an autocatalytic fashion and by pepsin itself. In humans there are two related forms of pepsin, Pepsinogen I (also known as pepsinogen A), and Pepsinogen II (also known as Pepsinogen B or progastricsin). Mouse anti Human pepsinogen 1 antibody, clone 8003 has an affinity of 4 x 1010 l/mol human Pepsinogen I.
Mouse anti Human parathyroid hormone monoclonal antibody, clone BGN/1F8 recognizes human parathyroid hormone (PTH) also known as Parathyrin. PTH is a hormone produced by the parathyroid gland responsible for the regulation of calcium and phosphorus concentrations in extracellular fluid (Brown 1983).Human parathyroid hormone is produced in the parathyroid gland as a 115 amino acid single chain polypeptide, bearing a 25 amino acid signal peptide, a 6 amino acid pro-peptide sequence and an 84 amino acid mature hormone. (Keutmann et al. 1978)
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
BGN/1F8
Concentration:
IgG 1 mg/ml
Format:
Purified
Storage buffer:
PBS with <0.1% sodium azide
Storage:
aliquots -20°C. Thawed 2-8°C
References 1:
Nechama M et al. J Clin Invest 2009; 119: 3102-14
References 2:
Galitzer H et al. Kidney Int 2010; 77: 211-8
References 3:
Meir T et al. Am J Physiol Renal Physiol 2009; 297: F1192-8
References 4:
Ferre S et al. J Clin endocrinol Metab 2013; 98: 4089-96
Mouse anti Human N-cadherin antibody, clone 13A9 recognizes neural cadherin, otherwise known as CD325, a calcium dependent cell-cell adhesion glycoprotein, and member of the cadherin superfamily, which links to the actin cytoskeleton via catenins, and plays a role in cell-matrix adhesion, cell growth and differentiation, and the establishment of left-right asymmetry. Mouse anti Human N-cadherin antibody, clone 13A9 does not recognize E-cadherin, M-cadherin or P-cadherin (Knudsen et al. 1995). Mouse anti Human N-cadherin antibody, clone 13A9 is a reliable marker for the differential diagnosis of pleural mesotheliomas and lung adenocarcinomas, when used in conjunction with E-cadherin (Han et al. 1997).
Mouse anti Human Mucin 2 antibody, clone 996/1 recognizes human Mucin 2 (MUC-2), and shows no cross-reactivity with MUC-1, MUC-3 or MUC-4. In formalin fixed, paraffin embedded tissue sections Mouse anti Human Mucin 2 antibody,clone 996/1 reveals high levels of expression in colon, liver and prostate tissues (Durrant et al. 1994). Mouse anti Human Mucin 2 antibody, clone 996/1 recognizes malignant colonic mucosa as well as normal mucosa. Epitope mapping indicates that Mouse anti Human Mucin 2 antibody, clone 996/1 recognizes a sequence PTGTQ within the mucin 2 tandem repeat region (Uray et al. 1999).
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
996/1
Concentration:
IgG 1 mg/ml
Format:
Purified
Storage buffer:
PBS with <0.1% sodium azide
Storage:
aliquots -20°C. Thawed 2-8°C
References 1:
Filipe MI et al. Eur J Cancer Prev 1996; 5: 287-95
References 2:
Paulsen FP et al. Invest Opthalmol Vis Sci 2003; 44: 1807-13
References 3:
Price MR et al. Int J Oncol 1999; 15: 803-9
References 4:
Tugyi R et al. PNAS 2005; 102: 413-8
References 5:
Uray K et al. Arch Biochem Biophys 2000; 378: 25-32
Mouse anti Human MMP-9 Activated antibody, clone 4A3 recognizes the active form of human matrix metalloproteinase 9 (MMP-9). The MMPs are zinc-dependent endopeptidases responsible for degrading the extracellular matrix. They are also involved in cell proliferation, migration, differentiation and apoptosis. Most MMPs are synthesised as inactive zymogens and a propeptide region must be cleaved off before the enzyme becomes active. Their expression is increased dramatically in a variety of cancer types, where it indicates invasive disease and a poor prognosis. MMP-9 is a gelatinase, cleaving type IV collagen and gelatin. The gelatinases have an additional gelatin-binding domain inserted in the catalytic domain. MMP-9, alongside other MMPs, plays a role in normal tissue remodeling such as embryonic development, ovulation, mammary gland involution and wound healing. It is important in the early stages of tumor invasion as it degrades the type IV collagen in the basement membrane
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
4A3
Concentration:
IgG 0.5 mg/ml
Format:
Purified
Storage buffer:
PBS with <0.1% sodium azide
Storage:
aliquots -20°C. Thawed 2-8°C
References 1:
Duncan ME et al. Eur J Biochem 1998; 258: 37-43
References 2:
Selemetjev S et al. Am J Clin Pathol 2016; pii: aqw184 (Epub ahed of print)
Mouse anti Human MALT1 antibody, clone 50 recognizes human mucosal-associated lymphoid tissue lymphoma translocation 1 (MALT1), also known as MALT lymphomaassociated translocation and paracaspase. MALT1 is an 824 amino acid ~93 kDa protein belonging to the peptidase C14B family containing a single death domain and two Ig-like CD-type domains. In normal lymphocytes, MALT1 plays an important role in antigen receptor-mediated lymphocyte activation. In T-cells, MALT1 is recruited by activated CARMA1, along with Bcl-10, to form a CARMA1-Bcl10-MALT1 (CBM) complex which is involved in the activation of NF-kappaB (Yang et al. 2013).
Mouse anti Human MAdCAM-1 antibody, clone 17F5 recognizes human mucosal addressin cell adhesion molecule 1 (MAdCAM-1) a ~60 kDa transmembrane protein that belongs to the immunoglobulin superfamily. MAdCAM-1 is strongly expressed on mucosal lymphoid tissue and high endothelial venules of Peyer's patches. Human MAdCAM-1 interacts with the beta 7 integrin LPAM-1 (alpha4beta7), CD62L, and VLA-4 (alpha4beta1) and is involved in lymphocyte trafficking, Mouse anti Human MAdCAM-1 antibody, clone 17F5 recognizes the mucin domain of MAdCAM-1. This antibody does not block the function of MAdCAM-1 (Leung, E. et al. 2004).
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
17F5
Concentration:
IgG 1 mg/ml
Format:
Purified
Storage buffer:
PBS with <0.1% sodium azide
Storage:
aliquots -20°C. Thawed 2-8°C
References 1:
Leung E et al. Immunol Cell Biol 1996; 74: 490-6
References 2:
Leung E et al. Immunol Cell Biol 2004; 82: 400-9
References 3:
Guerra-Perez N et al. J Immunol 2015; 194: 2415-23
Fibronectin receptor, also designated VLA-5, is a 130/150 kDa protein. The protein functions as a receptor for fibronectin and mediates binding of B and T lymphocytes to fibronectin. Fibronectin is an extracellular matrix glycoprotein that functions in cell adhesion and migration in wound healing, embryonic development and malignant transformation. The fibronectin receptor is expressed on monocytes and monocytoid cell lines, leukocytes, memory T cells, fibroblasts, platelets and muscle cells.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
NKI-SAM1
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
te Velde; A et al. J Immunol 1988; 140: 1548
References 2:
Danen, E et al Soc for Biochem and Mol Biol 1995, 270: 21612
Mouse anti Human Lysozyme antibody, clone BGN/0696/5B1 recognizes lysozyme, an enzyme that is present in mucosal secretions such as saliva and tears and is involved with non-specific organism defence. It functions by hydrolyzing 1,2-beta linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues such as those found in the walls of various Gram-positive bacteria.
Mouse anti Human HIF1 alpha antibody, clone Halpha111a recognizes human Hypoxia-inducible factor 1-alpha otherwise known as HIF1 alpha (HIF1A) or ARNT interacting protein, a widely expressed bHLH-PAS transcription factor, which acts as a critical regulatory protein in the host response to hypoxia (Kubis et al. 2005).
Mouse anti Human cytokeratin 19 antibody, clone A53-B/A2 recognizes the rod domain (aa 312-335) of human cytokeratin 19 (Böttger; et al. 1995) also known as keratin 19 encoded by the KRT19 gene. Cytokeratin 19 is a 400 amino acid intermediate filament protein lacking a C-terminal tail domain, in contrast to all other intermediate filament proteins.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
A53-B/A2
Concentration:
IgG 1 mg/ml
Format:
Purified
Storage buffer:
PBS with <0.1% sodium azide
Storage:
aliquots -20°C. Thawed 2-8°C
References 1:
Karsten U et al. Eur J Cancer Clin Oncol 1985; 21: 733-40
References 2:
Goletz S et al. J Cell Sci 1997; 110: 1585-96
References 3:
de Neergaard M et al. Am J Pathol 2010; 176: 1229-40
Mouse anti Human Corticotrophin Releasing Factor antibody, clone 4H9 recognizes human corticotrophin releasing factor (CRF), a hormone found mainly in the hypothalamus that regulates the release of corticotrophin from the pituitary gland. The unique lysine residue (Lys-36) is likely to be involved in the antigenic determinant as lysine modification by CA blocks binding completely. Human serum, containing CRF-binding protein, also inhibits the binding of this antibody to CRF.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
4H9
Concentration:
IgG 1 mg/ml
Format:
Purified
Storage buffer:
PBS with <0.1% sodium azide
Storage:
aliquots -20°C. Thawed 2-8°C
References 1:
Kravchenko IV & Furalev VA. Hybridoma 1994; 13: 59-64
References 2:
DeBellis A et al. J Clin Endocrinol Metab 2012; 97: 3684-90
Mouse anti Human chymotrypsin antibody, clone 4E1 recognizes human chymotrypsin-C, also known as chymotrypsin or caldecrin. Chymotrypsin is a 239 amino acid ~30 kDa protease with an additional 13 amino acid propeptide region and a 16 amino acid signal peptide
Mouse anti Human CD98 antibody, clone 44D7 reacts with the heavy chain of the CD98 molecule. The same epitope as recognized by clone 4F2. This antibody binds to all cell lines in culture and to activated T-cells.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
44D7
Concentration:
IgG 1 mg/ml
Format:
Purified
Storage buffer:
PBS with <0.1% sodium azide
Storage:
aliquots -20°C. Thawed 2-8°C
References 1:
Letarte M et al. Biochem Cell Biol 1986; 64: 1160-9
References 2:
Jaramillo R et al. J Allergy Clin Immunol 2013; 131: 405-11
References 3:
Mansson A et al. J Investig Allergol Clin Immunol 2010; 20: 476-83
References 4:
Millrud CR et al. PLoS One 2012; 7: e51120
References 5:
Garrigues HJ et al. Virology 2014; 464-465:118-133
Mouse anti human CD92 antibody, clone VIM-15b recognizes the C-terminal variant of human CDw92 (isoform 3), also known as CTL1, a 70 kDa multi-pass membrane protein, expressed by monocytes, neutrophils, certain myeloid and T cell lines, and weakly by endothelial cells, fibroblasts and epithelial cells. CDw92 is a member of the choline transporter-like protein family, so called due to their involvement in the efficient supply/transport of the natural amine choline, a vital cell nutrient required for the synthesis of cell membrane phospholipid components and the neurotransmitter acetylcholine.
ECAD (syn, CD325), 120 kDA, chromosome 16q22.1 (CDH1 gene), is a critical regulator of epithelial junction formation. It interacts with the cytoskeleton through several associated proteins. The ECAD internal domain binds with alpha, beta, gamma and p120 catenins to anchor the ECAD complex to the actin cytoskeleton of the cell. ECAD is expressed in virtually all epithelial cells except for adrenocortical cells. The expression in liver cells is weaker than in most other epithelia. ECAD is also expressed in melanocytes (adhering to squamous epithelial cells). Pretreatment: Heat induced epitope retrieval in 10 mM citrate buffer , pH6.0 for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based detection is recommended. Control tissue Pancreas, lung adenocarcinoma, breast. Staining Membranous
The anti-CD43 antibody recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So, a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma. Pretreatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0, or in 50 mM Tris buffer pH9.5, for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based detection is recommended. Control tissue Tonsil. Staining Membranous.
Antibody Isotype:
IgG2, kappa
Monosan Range:
MONOSAN
Clone:
DF-T1
Concentration:
n/a
Format:
Concentrate
Storage buffer:
Bioreactor Concentrate with 0.05% Azide
Storage:
2-8°C
References 1:
Leong A, Cooper K, Leong F. London: Oxford University Press; 1999. p. 91-2.
References 2:
Stross WP, Warnke RA, Flavell DJ, Flavell SU, Simmons D, Gatter KC, et al. J Clin Pathol 1989; 42:953-61.
References 3:
de Smet W, Walter H, van Hove L.. Immunology 1993;79:46-54.
Recognizes a protein of -55kDa, identified as SOX I 0. This MAb is highly specific and does not cross-react with other members of the SOX-family. SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA binding activity. SOX-10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. It is expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S I 00 protein. Commonly used melanoma markers, such as anti-HMB-45 and anti-Melan-A, are poorly expressed in desmoplastic melanomas while SOX-10 is moderately to strongly expressed in desmoplastic melanomas. SOX- 10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes, and myoepithelial cells of salivary, bronchial and mammary glands. SOX-I 0 expression is also observed in mast cells. Pretreatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0, for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based detection is recommended. Control tissue Melanomas, breast carcinomas, gliomas. Staining Nuclear
Antibody Isotype:
IgG2b, kappa
Monosan Range:
MONOSAN
Clone:
SOX10/1074
Concentration:
n/a
Format:
Concentrate
Storage buffer:
Bioreactor Concentrate with 0.05% Azide
Storage:
2-8°C
References 1:
Mohamed A, et al, Mol Morphol. 2013; 21(6):506-10.
The expression of MDM2 is itself, induced by p53 and may be a way for p53 to self-regulate its activity during the normal cell cycle. However, overexpression of MDM2 results in the loss of p53-regulated growth control and consequently, deregulated cell proliferation. MDM2 also binds to the Retinoblastoma tumor suppressor protein (Rb) and inhibits its growth regulatory function. MDM2 can directly augment proliferation by binding to two transcription factors E2F1 and DP1 and stimulating the activity of the S-phase inducing E2F1/DP1 heterodimer. MDM2 migrates at a reduced molecular weight of ~95 kDa. The SMP14 clone has been reported to recognize human, mouse and rat MDM2 while exhibiting a slight cross-reactivity with cytokeratins 6, 14 and 16 in some experimental systems. In the immunoprecipitation application, SMP14 has been reported to precipitate MDM2 and p53-MDM2 complexes. MCF7 human breast carcinoma cells (ATCC HTB-22) and NIH/3T3 mouse fibroblasts (ATCC CRL-1658) are suggested as western blot and immunoprecipitation positive controls. SMP14 has been reported to be useful for the immunohistochemical staining of acetone-fixed, frozen sections and of formalin-fixed, paraffin-embedded tissue sections. In addition to a nuclear staining of MDM2, cytoplasmic staining may also be observed which is likely to be attributable to the slight cross reactivity of the SMP14 clone with cytokeratins. Control tisse Breat carcinoma. Staining Nuclear
RIV12 reacts with CD1b, a 44KDa type 1 glycoprotein associated with beta2-microglobulin. It is expressed on dendritic cells, Langerhans cells, thymocytes and T acute lymphoblastic leukemia cells. CD1, type 1 membrane protein, has structural similarity to the MHC class 1 molecule and has been shown to present lipid antigens for recognition by T lymphocytes. CD1b is also expressed in Langerhans interdigitating cells.
Transcription factors containing the POU homeo domain have been shown to be important regulators of tissue-specific gene expression in lymphoid and pituitary differentiation and in early mammalian development. POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. Oct-3 (also known as Oct-4) is a mammalian POU transcription factor expressed by early embryo cells and germ cells. Oct-3/4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo. A critical amount of Oct-3/4 is required to sustain stem-cell self renewal, and up or down regulation induce divergent developmental programs. Two isoforms of Oct-3, termed Oct-3A and Oct-3B, are generated by alternative splicing. The gene which encodes Oct-3/4 maps to human chromosome 6p21.3. Oct-3/4 (C-10) is recommended for detection of Oct-3A (Oct-4) and Oct-3B of mouse, rat and human origin by Western Blotting, immunoprecipitation, immunofluorescence, and paraffin immunohistochemistry. Pre-treatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0, or in 50 mM Tris buffer pH9.5, for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based detection is recommended. Control tissue Seminoma or embryonal carcinoma. Staining Nuclear
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
C-10
Concentration:
n/a
Format:
Purified
Storage buffer:
Purified antibody in PBS with 0.2 % BSA and 15mM sodium azide
Storage:
2-8°C
References 1:
Drocourt, L., et al. 2002, J. Biol. Chem. 277: 25125-25132.
References 2:
Fong, Y.W., et al. 2011, Cell 147: 120-131.
References 3:
Wang, J., et al. 2011, Cancer Res. 71: 7238-7349.
References 4:
Rijlaarsdam, M.A., et al. 2011, Br. J. Cancer 105: 854-863.
Recognizes a phosphor-protein of 45kDa, identified as MyoD1. The epitope of this MAb maps between amino acid 180-189 in the C-terminal of mouse MyoD1 protein. It does not cross react with Myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilms tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and in rhabdomyosarcomas, neuroblastomas, Ewings sarcomas and alveolar soft part sarcomas. Pre-treatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0, or in 50 mM Tris buffer pH9.5, for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based detection is recommended. Control tissue Rhabdomyosarcoma. Staining Nuclear (only nuclear staining should be considered as evidence of skeletal muscle differentiation).
Antibody Isotype:
IgG1, kappa
Monosan Range:
MONOSAN
Clone:
5.8A
Concentration:
n/a
Format:
Concentrate
Storage buffer:
Bioreactor Concentrate with 0.05% Azide
Storage:
2-8°C
References 1:
Thulasi R et. al. Cell Growth and Differentiation, 1996, 7(4):531-41.
References 2:
Wesche WA et. al. American Journal of Surgical Pathology, 1995, 19(3):261-9.
The Yes-associated protein, otherwise known as YAP, is a 14-3-3 binding molecule that was originally recognized by virtue of its ability to bind to the SH3 domain of Yes. The binding of YAP to 14-3-3 requires the phosphorylation of a homologous serine residue (Ser 112) in the YAP 14-3-3 binding motif. The highly conserved and ubiquitously expressed 14-3-3 proteins regulate differentiation, cell cycle progression and apoptosis by binding intracellular phosphoproteins involved in signal transduction. YAP may link events at the plasma membrane and cytoskeleton to inhibition of transcription in the nucleus in a manner regulated by 14-3-3 proteins. YAP shares homology with the WW domain of TAZ, transcriptional co-activator with PDZ binding motif, which functions as a transcriptional co-activator by binding to the PPXY motif present in transcription factors. YAP is expressed at high levels in the lung, placenta, prostate, ovary and testis. Pretreatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0, for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based detection is recommended. Control tissue Lung, placenta, prostate, ovary, testis. Staining Nuclear.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
63.7
Concentration:
n/a
Format:
Concentrate
Storage buffer:
PBS with less than 0.1% sodium azide and 0.1% gelatin
Storage:
2-8°C
References 1:
Ren, Y.R., et al. 2011. S, J. Biol. Chem. 286: 11960-11969.
References 2:
Ellison, D.W., et al. 2011. Acta Neuropathol. 121: 381-396.
References 3:
Cordenonsi, M., et al. 2011. Cell 147: 759-772.
References 4:
Ren, Y.R., et al. 2012. J. Proteome Res. 11: 5301-5310.
References 5:
Vigneron, A.M. and Vousden, K.H. 2012. EMBO J. 31: 471-480.
Mutations within the BRCA1 gene, localized to chromosome 17q, are believed to account for approximately 45% of families with increased incidence of both early-onset breast cancer and ovarian cancer. The BRCA1 gene is expressed in numerous tissues, including breast and ovary, and encodes a predicted protein of 1,863 amino acids. This protein contains a RING domain near the N-terminus and appears to encode a tumor suppressor. BARD1 (BRCA1-associated RING domain protein 1) and BAP1 (BRCA1-associated protein 1) have both been shown to bind to the N-terminus of BRCA1 and are potential mediators of tumor suppression. BARD1 contains an N-terminal RING domain and three tandem ankyrin repeats. The C-terminus of BARD1 contains a region with sequence homology to BRCA1, termed the BRCT domain. BAP1 is a ubiquitin hydrolase and has been shown to enhance BRCA1-mediated cell growth suppression. Pre-treatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0, or in 50 mM Tris buffer pH9.5, for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Control tissue Pancreas, breast carcinoma, ovarian carcinoma. Staining Nuclear and cytoplasmic.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
C-4
Concentration:
n/a
Format:
Concentrate
Storage buffer:
PBS with < 0.1% sodium azide and 0.1% gelatin
Storage:
2-8°C
References 1:
Hall, J.M., et al. 1990, Science 250: 1684-1689.
References 2:
Yoshikawa,Y., et al. 2012, Cancer Sci. 103: 868-874.
References 3:
Gammon, B., et al. 2013, J. Cutan. Pathol. 40: 538-542.
References 4:
Kerl, K., et al. 2013, Am. J. Dermatopathol. 35: 151-158.
References 5:
Popova T., et al. 2013, Am. J. Hum. Genet. 92: 974-980.
Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of JAK kinases which then leads to tyrosine phosphorylation of proteins that have been designated Stats (signal transducers and activators of transcription). The first members of this family to be described include Stat1? p91, Stat1? p84 (a form of p91 that lacks 38 COOH-terminal amino acids) and Stat2 p113. Stat1 and Stat2 are induced by IFN-? and form a heterodimer which is part of the ISGF-3 transcription factor complex. Stat3, which becomes activated in response to epidermal growth factor (EGF) and interleukin-6 (IL-6), but not interferon-? (IFN-?) or Stat4, is an additional member of this family. It has been suggested that the phosphorylated forms of both Stat3 and Stat4 form homodimers as well as heterodimers with the other members of the Stat family, and that differential activation of different Stat proteins in response to different ligands should help to explain specificity in nuclear signaling from the cell surface. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Other members of the Stat family include Stat5, which has been shown to be activated by Prolactin and by IL-3, and Stat6 (also designated IL-4 Stat), which is involved in IL-4-activated signaling pathways. Pretreatment: Heat induced epitope retrieval in10 mM citrate buffer, pH6.0, for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Control tissue Urinary bladder. Staining Nuclear.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
D1
Concentration:
n/a
Format:
Concentrate
Storage buffer:
PBS Buffer, with less than 0.1% sodium azide and 0.1% gelatin
Storage:
2-8°C
References 1:
Zhong, Z., et al. 1994. Science 264: 95-98.
References 2:
Darnell, J.E., et al. 1994. Science 264: 1415-1421.
References 3:
Hou, J., et al. 1994. Science 265: 1701-1706.
References 4:
Yamamoto, K., et al. 1994. Mol. Cell. Biol. 14: 4342-4349.
This MAb is specific to lambda light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant. Pretreatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0, or in 50 mM Tris buffer pH9.5, for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based detection is recommended. Control tissue HEK293T, Tonsil, Spleen
Originally, the 2G9 monoclonal antibody (mAb) was described as identifying a 15 kDa protein found in the cytoplasmic granules of cytotoxic T cells that might be part of a larger 40 kDa molecule, ubiquitously expressed, named p40-TIA-1 and often referred to as TIA-1 in the literature (1, 2). Now, however, there is evidence that the 2G9 mAb identifies a 17 kDa cytoplasmic granule membrane protein named GMP-17 that has no similarity with p40-TIA-1 (3). The GMP-17 antigen is a 165 amino acid protein with 4 transmembrane domains: but it is not a typical member of the fourtransmembrane superfamily. It is identical with previously identified cytotoxic granule proteins called NKG7 and GIG-1 for GCSF induced gene protein 1 , isolated from NK cells and granulocyte-colony-stimulatingfactor- treated mononuclear cells, respectively (4, 5). Pretreatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0, for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based detection is recommended. Control tissue Tonsil. Staining granular
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
2G9A10F5
Concentration:
n/a
Format:
Purified
Storage buffer:
Liquid purified Ascites, purified with Protein G Chromatography with 15mM Sodium Azide
Storage:
2-8°C
References 1:
Anderson, P. et al, 1990, J. Immunol., 2, 144, 574.
References 2:
Tian, Q., et al, 1991, Cell, 67, 629-639.
References 3:
Medley, et al, 1996, Proc. Natl. Acad. Sci. U S A, 93, 2, 685-9.
References 4:
Turman, M.A., et al, 1993, Hum. Immunol., 36, 1, 34-40.
References 5:
Shimane, et al, 1994, Biochem Biophys Res Commun., 199, 1, 26-32.
The LIM-only (LMO) proteins, LMO1 and LMO2, are nuclear factors that are characterized by a conserved LIM domain. The LIM domain consists of a cysteine-rich zinc-binding motif that is present in a variety of transcription factors, including the LIM homeobox (LHX) proteins expressed in the central nervous system and involved in cell differentiation. LMO1 and LMO2 are expressed in the adult CNS in a cell type-specific manner, where they are differentially regulated by neuronal activity and are involved in regulating the cellular differentiated phenotype of neurons. LMO2 lacks a specific DNA-binding homeobox domain but rather assembles into transcriptional regulatory complexes to mediate gene expression by interacting with the widely expressed nuclear LIM interactor (NLI). NLI, known also as CLIM-1, and the related protein CLIM-2 facilitate the formation of heteromeric LIM complexes and also enhance the nuclear retention of LIM proteins. LMO2 and the related protein LMO4 are expressed in thymic precursor cells. LMO4 is also expressed in mature T cells, cranial neural crest cells, somite, dorsal limb bud mesenchyme, motor neurons, and Schwann cell progenitors. Pretreatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0 for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based detection is recommended. Control tissue Tonsil, salivary gland. Staining Cytoplasmic
Antibody Isotype:
IgG
Monosan Range:
MONOSAN
Clone:
1A9-1
Concentration:
200 ug IgG1/ml
Format:
Concentrate
Storage buffer:
PBS with < 0.1% sodium azide and 0.1% gelatin
Storage:
2-8°C
References 1:
Zhang, J., et al. 2009. Blood 113: 4586-4594.
References 2:
Copie-Bergman, C., et al. 2009. J. Clin. Oncol. 27: 5573-5579.
References 3:
Sonmez, M., et al. 2009. Hematology 14: 220-223.
References 4:
Cobanoglu, U., et al. 2010. Hematology 15: 132-134.
References 5:
Li, D., et al. 2012. Ann. Diagn. Pathol. 16: 335-343.
This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Pretreatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0, or in 50 mM Tris buffer pH9.5, for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based detection is recommended. Control tissue Melanoma, normal skin. Staining Cytoplasmic
Antibody Isotype:
IgG2b, kappa
Monosan Range:
MONOSAN
Clone:
M2-9E3
Concentration:
n/a
Format:
Concentrate
Storage buffer:
Bioreactor Concentrate with 0.05% Azide
Storage:
2-8°C
References 1:
Kawakami Y, et. al. Journal of Immunological Methods, 1997, 202(1):13-25.
References 2:
Marincola FM, et. al. J of Immunotherapy with Emphasis on Tumor Immunol, 1996, 19(3):192-205.
IGF-1 Receptor recognizes human CD221, a 155kD receptor tyrosine kinase, also known as Insulin-like growth factor I receptor (IGF-I Receptor). CD221 is composed of two extracellular alpha-subunits and two transmembrane beta-subunits. Clone 1H7 recognizes an epitope in the alpha subunits of CD221, demonstrated by Western blotting (1). CD221 is expressed in a range of tissues, including kidney, liver, placenta, mammary gland, brain, ovary and skin. The ligands for CD221 include IGF-I and IGF-II, which bind to CD221 and activate tyrosine kinase activity, resulting in phosphorylation of several intracellular signaling proteins. Clone 1H7 is reported to partically block binding of IGF-I and IGF-II to CD221 (1). Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Pretreatment: protein digestion (Trypsin or Pronase) is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based detection is recommended.
Antibody Isotype:
IgG
Monosan Range:
MONOSAN
Clone:
1H7
Concentration:
n/a
Format:
Purified
Storage buffer:
PBS with azide
Storage:
2-8°C
References 1:
Li, S.L. et al. (1993) Biochem. Biophys. Res. Commun. 196:92-98.
References 2:
Beauvais, D.M. and Rapraeger, A.C. (2010) J Cell Sci. 2010 Nov 1;123: 3796-807.
Recognizes a protein of 67kDa, which is identified as estrogen receptor (ER) alpha. The ER gene consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains, labeled A through F. This antibody strongly stains the nucleus of epithelial cells in breast carcinomas. The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to antiestrogen (e.g. tamoxifen) therapy. Pretreatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0, or in 50 mM Tris buffer pH9.5, for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-rabbit secondary antibody-based detection is recommended. Control tissue Breast carcinoma. Staining nuclear.
Antibody Isotype:
IgG
Monosan Range:
MONOSAN
Clone:
ZR2
Concentration:
n/a
Format:
Concentrate
Storage buffer:
Tissue culture supernatant with 0.2% BSA and 15mM sodium azide
Calretinin is a 29 kDa calcium-binding protein, member of the family of so-called EF-hand proteins, to which also the S-100 proteins belong. Calretinin is abundantly expressed in neurons. Outside the nervous system, calretinin is found in mesothelial cells, steroid producing cells (adrenal cortical cells, testicular Leydig cells, ovarian theca internal cells), testicular Sertoli cells, rete testis, ovarian surface epithelium, some neuroendocrine cells, breast glands, eccrine sweat glands, hair follicular cells, thymic epithelial cells, endometrial stromal cells and fat cells. In calretinin positive cells, the protein is generally found in both cytoplasm and nuclei. Pre-treatment: Heat induced epitope retrieval in 10 mM citrate buffer pH6.0 for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based detection is recommended. Control tissue Brain tissue and mesothelioma. Staining cytoplasmic, nuclear.
The PAX-5 gene is essential for B-cell differentiation. There are at least four isoforms, of which PAX-5a has been most studied. PAX-5 encodes the 50 kDa B-cell specific activator protein, BSAP. PAX-5 is expressed by pro-, pre-and mature B-cells, but is downregulated during terminal differentiation of plasma cells. PAX-5 influences the expression of other B-cell specific genes, including CD19 and CD20 and CD79a, preceding the expression of CD20. PAX-5 is silenced at the plasma cell stage under the influence of B-lymphocyte-induced maturation protein-1 (PRDM1). PAX-5 is expressed during mouse embryogenesis within the developing brain in a way that is temporarily and spatially tightly condoled. PAX-5 deficient mice show deformation of the mid-brain. Expression in human embryogenesis occurs in the mesoencephalon and spinal cord. Pretreatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0, for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. (dilution 1:100 - 1:200) The optimal dilution for a specific application should be determined by the investigator. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based detection is recommended. Control tissue Tonsil; staining nuclear
CD 19 is expressed only on B-cells and follicular dendritic cells. It is a specific and sensitive marker of B-cells widely expressed from early pre-B stages, normal B-cells and normal plasma cells (staining is weaker than normal B-cells and a subpopulation may lack expression). It is considered a positive regulator of both intrinsic and stimulus-dependent pathways in B-lymphocytes. CD19 is useful in identification of B-cell lineage of majority of B-cell neoplasms but appears to be less useful in subclassifying of B-cell neoplasms in histological material. It appears to be potentially useful additional marker of follicular dendritic cell tumours. Pretreatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0, for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based detection is recommended. Control tissue Tonsil or lymph node. Staining membranous
As one of the cyclin-dependent kinase inhibitors that inhibit cylcin-dependent kinases 4 and 6, p16INK4A is encoded by tumor suppressor gene CDKN2A. The tumor suppressor p16INK4A plays an important role in cell cycle regulation. Increased expression of the p16 gene, which is seen as organisms age, reduces the proliferation of stem cells. This reduction in the division and production of stem cells protects against cancer while increasing the risks associated with cellular senescence. Mutations in the p16 gene associated with loss or over expression of the protein are associated with increased risk of a wide range of cancers and cancer precursor lesions. The Immunohistochemical identification of p16 is particularly relevant in uterine cervical lesions: Development of dysplasia is closely related to human papilloma virus (HPV) infection. Pretreatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0, for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections.Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based detection is recommended. Control tissue Human cervical cancer, tonsil. Staining cytoplasmic and nuclear.
The progesterone receptor (PgR) is an estrogen regulated protein. It has been proposed that expression of PgR determination indicates a responsive estrogen receptor (ER) pathway, and therefore, may predict likely response to endocrine therapy in human breast cancer. A number of studies have shown that PgR determination provides supplementary information to ER, both in predicting response to endocrine therapy and estimating survival. PgR has proved superior to ER as a prognostic indicator in some studies. Pretreatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0 for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-rabbit secondary antibody-based detection is recommended. Control tisse Breast, breast ducatl carcinoma. Staining Nuclear
Antibody Isotype:
IgG
Monosan Range:
MONOSAN
Clone:
ZR4
Concentration:
n/a
Format:
Concentrate
Storage buffer:
Tissue culture supernatant with 0.2% BSA and 15mM sodium azide
p63 consists of two major isoforms -TAp63 and ?Np63. These isoforms differ in the structure of the N-terminal domains. The TAp63 isoform (identified by anti-p63 antibody) contains a transactivation- competent TAdomain with homology to p53, which regulates the expression of the growth -inhibitor y genes. In contrast, ?Np63 isoform (identified by anti-p40 antibody) contains an alternative transcriptionally- inactive ?N domain, which antagonizes the activity of TAp63 and p53. The p40 (clone ZR8) recognizes exclusively ?Np63 but not TAp63. p40 is a squamous cell carcinoma specific antibody. It reacts with the vast majority of cases of squamous cell carcinomas of various origins, but not with adenocarcinomas. It is particularly useful in differentiating lung squamous cell carcinoma from lung adenocarcinoma. p40 antibody can also be used as an alternative basal cell/myoepithelial cell marker, which has similar sensitivity and specificity as that of p63 antibody. Therefore, p40 antibody may also be used as an alternative immunohistochemical marker for determining prostate adenocarcinoma vs. benign prostate glands and for determining breast intraductal carcinoma v s. invasive breast ductal carcinoma. Pretreatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0, or in 50 mM Tris buffer pH9.5, for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-rabbit secondary antibody-based detection is recommended. Control tissue Lung squamous cell carcinoma. Staning nuclear
Antibody Isotype:
IgG
Monosan Range:
MONOSAN
Clone:
ZR8
Concentration:
n/a
Format:
Purified
Storage buffer:
Purified antibody in 0.2% BSA and 15mM sodium azide.
Human prostein is a 553 aa protein identified by cDNA library substraction abd subsequent highthroughput microarray ascreening of prostate cancer. Prostein has multiple transmemberane domains. Prostein has been shown to be uniquely expressed in normal and cancerous prostatic tissues. By immunohistochemistry, prostein is expressed in the vast majority of normal and malignant prostatic tissues, regardless of grade and metastatic status. No protein expression is detected in normal and malignant tissue samples representing the great majority of essential tissues and tumors. Prostein is expressed in most of poorly differentiated prostatic carcinoma, including small cell prostate carcinoma. Prostein is more specific and sensitive for prostatic carcinomas than PSA and PSAP. Pretreatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0, for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-rabbit secondary antibody-based detection is recommended. Control tissue Normal prostate or postate carcinoma. Staining cytoplasmic
Antibody Isotype:
IgG
Monosan Range:
MONOSAN
Clone:
ZR9
Concentration:
n/a
Format:
Concentrate
Storage buffer:
Tissue culture supernatant with 0.2% BSA and 15mM sodium azide
CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions.
CD11b is an alpha chain integrin that is expressed as heterodimer noncovalently associated with CD18. CD11b is also designated as CR3, Mac-1, Mo-1 in integrin alphaM subunit and has a molecular weight of 165-170 kDa. The protein mediates PMN and monocytes adherence to endothelium and subsequently PMN extravasation to sites of inflammation. CD11b/CD18 interacts with both matrix and cell surface proteins including the complement component iC3b, extracellular matrix proteins and the third extracellular domain of CD54. CD11b is expressed on human NK-cells, monocytes, granulocytes, T-cells, B-cells and dendritic cells. After activation the expression is upregulated. Bear-1 reacts with the CD11b alpha chain of the CD11b/CD18 (p165, 95) integrin heterodimer complex.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
BEAR-1
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Keizer; G et al. Eur J Immunol 1985; 15: 1142
References 2:
Watanabe, A et al Int Arch Allergy Immunl 1992, 98: 308
The antibody reacts with a polymorphic determinant on HLA-DR molecules which is associated with DR3, DR5, and DRw6 (DRw52-like). It does not react with DRw8. The antibody reacts with part of the HLA-D molecules (29+34 kD). The determinant is strongly expressed on DR3 positive cells and more weakly on DR5 and DRw6 positive cells. The antibody is useful for HLA-DR typing and biochemical characterization of the DR52w positive molecules and functio¬nal studies. Cross reacts with Chimpanzee, Macaca-apes and gorilla.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
7.3.19.1
Concentration:
n/a
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Koning, F., et al., 1984, Human Immunology, 9, 221
References 2:
Koning, F., et al., 1985, Disease Markers 2, 75
References 3:
Ottenhoff, T.H.M., et al., 1985, Human Immunology, 13, 117
References 4:
Bontrop, R.E., et al., 1990, J. Exp. Med. 172, 53
References 5:
Verreck, F.A.W., et al., 1993, Eur. J. Immunol. 23, 1346
The antibody reacts with a monomorphic determinant on MHC class II, HLA-D region molecules. It detects the molecules of the HLA-DR, DP and DQ loci. It inhibits the mixed lymphocyte culture. It also reacts with MHC class II molecules of various animals and is a useful tool for studies with dogs. The antibody is useful for the identification of HLA-D positive molecules using either microcytotoxicity testing or immunofluorescence/immunoperoxidase procedures. Furthermore, it was found useful for studies on functional aspects of MHC class II molecules.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
7.5.10.1
Concentration:
n/a
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Koning, F., et al., 1984, Human Immunology 9, 221
References 2:
Koning, F., et al., 1985, Tissue Antigens 26, 100
References 3:
Doveren, R.F., et al., 1986, Tissue Antigen 27, 87-98
References 4:
Hauber, I., et al., 1995, J. Exp. Med 181, 1411-1423
References 5:
Groenenwegen, G., et al., 1986, J. Exp. Med 164, 131-143
CD57, also known as HNK-1 (human natural killer-1), is a cell surface carbohydrate epitope expressed on terminally differentiated T-cells and subsets of natural killer (NK) cells.1 It has also been identified on cells of neural crest origin.2 Anti-CD57 is often used to visualize the non-neoplastic bystander T-cells that may form rosettes around the neoplastic lymphocyte-predominant (LP) cells in nodular lymphocyte-predominant Hodgkin lymphoma (NLPHL).3
Antibody Isotype:
IgM-k
Monosan Range:
MONOSAN
Clone:
NK-1
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Kared H, et al. Cancer Immunol Immunother. 2016; 65:441-52
References 2:
Nielsen CM, et al. Front Immunol. 2013; 4:422
References 3:
Sattarzadeh A, et al. Exp Hematol Oncol. 2015; 4:27
NK-1 reacts with CD57, a 110 kDa antigen present on the surface of human NK-cells and Tcell subsets. In adult peripheral blood 20% of mononuclear cells are positive. Other positive tissues include peripheral nerves (myelin-associated glycoprotein) and prostate. Anti-CD57 also stains neuroendocrine cells and their derived tumors, including carcinoid tumor and medulloblastomas. Anti-CD57 can also be useful in separating type B3 thymoma from thymic carcinoma when combined with a panel that includes antibodies against GLUT1, CD5, and CEA.
The antibody reacts with the alpha chain the 150 kD molecule of the CD11c/CD18 Integrin complex (g150/95) which is expressed on monocytes and in a lower density on granulocytes and NK-cells of peripheral blood. It also reacts with many myelomonocytic and monocytic leukemias and (weakly) with part of the T8 positive cells (OKM1-like). The Integrins are important molecules in cellular adhesion and cellular interactions. Positive controle perifere blood lymphocytes
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
FK24
Concentration:
n/a
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Jansen, J., et al., 1984, Seminares in Hematology 21, 164
The antibody reacts with a 110 kD antigen present on the peripheral blood lymphocyte subset (primarily large granular lymphocytes) identified by the HNK-1 monoclonal antibody. It recognizes part of the Natural Killer cell population. CD57 is furthermore present on cells in neuroectodermal tissue
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
6-13-19-1
Concentration:
n/a
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Abo, T., and Balch, C.M., 1981, The Journal of Immunology 127, 1024
The antibody reacts with CD8, the 33 kD T8 antigen present on the human cytotoxic/suppressor T-cell subset. It strongly blocks the cytolytic activity of T8-positive CTLs. It also reacts with a subpopulation of Rhesus monkey lymphocytes which is identical to the population of human T8 positive cells.
Antibody Isotype:
IgG3
Monosan Range:
MONOSAN
Clone:
FK18
Concentration:
n/a
Storage buffer:
Serum free medium containing 1% FCS
Storage:
2-8°C
References 1:
Jansen, J., et al., 1984, Seminares in Hematology 21, 164
153-4D9 reacts with human CD84 (Mw 74 kDa). CD84 is expressed on mature B-cells and on Bcell line but not on plasma cell lines. Immunohistochemistry demonstrated that is strongly stains tissue macrophages. It is also expressed on platelets and at low levels on blood T-cells. It is a highly N-glycosylated protein and belongs to immunoglobulin superfamily. It may play a role in leukocyte activation but cellular expression does not significantly increase after activation. 153- 4D9 was clustered at the Vth WLDA.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
153-4D9
Concentration:
100 ug/ul
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Schlossman SF et al. (eds) Leukocyte Typing V. p.699-700, Oxford University Press, Oxford, (1995)
References 2:
De la Fuente, M, et al, Blood 90(6): 2398-2405 (1997)
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkynje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkynje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkynje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Antibody Isotype:
IgG2a kappa
Monosan Range:
MONOSAN
Clone:
MEM-57
Concentration:
1 mg/ml
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
CD3 is presented on all resting and activated human T-cells, on T-leukemia cells and a proportion of human thymocytes. CD3 plays an important role in the assembly and expression of the T-cell receptor complex. Furthermore it functions as a signal transductor. SPV-T3b reacts with human CD3 also designated as T3 with a molecular weight of 20-26 kDa. SPV-T3b recognizes also the T3 molecular complex.
The NSR Prestained Protein Ladder is a three colour protein standard with 13 prestained proteins covering molecular weights from 3.5 to 245 kilodaltons. It has been designed for use in most general applications for a protein ladder with a broad range of protein sizes, key reference bands and excellent Western transfer efficiency (tested with PVDF, nylon and nitrocellulose membranes).
Background Info:
Most of the proteins are covalently coupled with a blue chromophore apart from two reference bands, one stained red at 75 kDa and one stained green at 25 kDa for easy reading of the ladder and to aid in the monitoring of protein separation.
The NSR Prestained Protein Ladder is supplied ready to use in gel loading buffer.
PLEASE E-MAIL US TO REQUEST A FREE SAMPLE.
Arrange for our Protein ladder to be in your stores and receive a free vial. Email us for more details - tech@nktscientific.com.
Product Type:
NS Reagents Protein Ladder
Format:
Buffer: 20 mM Tris-phosphate, pH 7.5, 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, 15 % (v/v) Glycerol.
Storage Temp:
Store at 25°C, for 2 weeks or 4°C for 3 months. For long-term storage, store at -20°C.
The shelf life is 1 year from receipt when stored frozen, however the NSR Prestained Protein Ladder should be stable for many years if stored frozen and excessive freeze thaw cycles are avoided.
The fragments in our 50bp ladder range from 50-1,500 base pairs, with the 200, 500 and 1,200 base pair bands having increased intensity to act as key reference points. There are 17 bands in total.
The expected mass of DNA in each band (based on a total of 0.56 ug in a 5ul load) is provided for approximating the mass of DNA in sample bands of a similar size.
Our DNA ladders are a combination of a plasmid digested DNA and PCR products, providing precise fragments.
PLEASE E-MAIL US TO REQUEST A FREE SAMPLE. .
Arrange for our DNA ladders to be in your stores and receive 5 vials of any of our DNA ladders for free. Email us for more details - tech@nktscientific.com.
Background Info:
Our 50bp ladder is provided ready to use with orange G as the tracking dye.
Product Type:
NS Reagents DNA Ladder
Format:
Buffer: 10mM Tris-HCl (pH 8.0) and 10mM EDTA
Storage Temp:
Store at 25°C, for 6 months or 4°C for a year. For long-term storage, store at -20°C.
The fragments in our 50bp ladder range from 50-1,500 base pairs, with the 200, 500 and 1,200 base pair bands having increased intensity to act as key reference points. There are 17 bands in total.
The expected mass of DNA in each band (based on a total of 0.56 ug in a 5ul load) is provided for approximating the mass of DNA in sample bands of a similar size.
Our DNA ladders are a combination of a plasmid digested DNA and PCR products, providing precise fragments.
PLEASE E-MAIL US TO REQUEST A FREE SAMPLE. .
Arrange for our DNA ladders to be in your stores and receive 5 vials of any of our DNA ladders for free. Email us for more details - tech@nktscientific.com.
Background Info:
Our 50bp ladder is provided ready to use with orange G as the tracking dye.
Product Type:
NS Reagents DNA Ladder
Format:
Buffer: 10mM Tris-HCl (pH 8.0) and 10mM EDTA
Storage Temp:
Store at 25°C, for 6 months or 4°C for a year. For long-term storage, store at -20°C.
The fragments in our 1Kbp ladder range from 250-10,000 base pairs, with the 1K and 3K base pair bands having increased intensity to act as key reference points. There are 13 bands in total.
The expected mass of DNA in each band (based on a total of 0.5 ug in a 5ul load) is provided for approximating the mass of DNA in sample bands of a similar size.
Our DNA ladders are a combination of a plasmid digested DNA and PCR products, providing precise fragments.
PLEASE E-MAIL US TO REQUEST A FREE SAMPLE. .
Arrange for our DNA ladders to be in your stores and receive 5 vials of any of our DNA ladders for free. Email us for more details - tech@nktscientific.com.
Background Info:
Our 1Kbp ladder is provided ready to use with bromophenol blue & xylene cyanol FF included as tracking dyes.
Product Type:
NS Reagents DNA Ladder
Format:
Buffer: 10mM Tris-HCl (pH 8.0) and 10mM EDTA
Storage Temp:
Store at 25°C, for 6 months or 4°C for a year. For long-term storage, store at -20°C.
The fragments in our 1Kbp ladder range from 250-10,000 base pairs, with the 1K and 3K base pair bands having increased intensity to act as key reference points. There are 13 bands in total.
The expected mass of DNA in each band (based on a total of 0.5 ug in a 5ul load) is provided for approximating the mass of DNA in sample bands of a similar size.
Our DNA ladders are a combination of a plasmid digested DNA and PCR products, providing precise fragments.
PLEASE E-MAIL US TO REQUEST A FREE SAMPLE.
Arrange for our DNA ladders to be in your stores and receive 5 vials of any of our DNA ladders for free. Email us for more details - tech@nktscientific.com.
Background Info:
Our 1Kbp ladder is provided ready to use with bromophenol blue & xylene cyanol FF included as tracking dyes.
Product Type:
NS Reagents DNA Ladder
Format:
Buffer: 10mM Tris-HCl (pH 8.0) and 10mM EDTA
Storage Temp:
Store at 25°C, for 6 months or 4°C for a year. For long-term storage, store at -20°C.
The fragments in our 100bp ladder range from 100-1,500 base pairs, with the 500 and 1,500 base pair bands having increased intensity to act as key reference points. There are 11 bands in total.
The expected mass of DNA in each band (based on a total of 0.5 ug in a 5ul load) is provided for approximating the mass of DNA in sample bands of a similar size.
Our DNA ladders are a combination of a plasmid digested DNA and PCR products, providing precise fragments.
PLEASE E-MAIL US TO REQUEST A FREE SAMPLE. .
Arrange for our DNA ladders to be in your stores and receive 5 vials of any of our DNA ladders for free. Email us for more details - tech@nktscientific.com.
Background Info:
Our 100bp ladder is provided ready to use with orange G & xylene cyanol FF included as tracking dyes.
Product Type:
NS Reagents DNA Ladder
Format:
Buffer: 10mM Tris-HCl (pH 8.0) and 10mM EDTA
Storage Temp:
Store at 25°C, for 6 months or 4°C for a year. For long-term storage, store at -20°C.
The fragments in our 100bp ladder range from 100-1,500 base pairs, with the 500 and 1,500 base pair bands having increased intensity to act as key reference points. There are 11 bands in total.
The expected mass of DNA in each band (based on a total of 0.5 ug in a 5ul load) is provided for approximating the mass of DNA in sample bands of a similar size.
Our DNA ladders are a combination of a plasmid digested DNA and PCR products, providing precise fragments.
PLEASE E-MAIL US TO REQUEST A FREE SAMPLE.
Arrange for our DNA ladders to be in your stores and receive 5 vials of any of our DNA ladders for free. Email us for more details - tech@nktscientific.com.
Background Info:
Our 100bp ladder is provided ready to use with orange G & xylene cyanol FF included as tracking dyes.
Product Type:
NS Reagents DNA Ladder
Format:
Buffer: 10mM Tris-HCl (pH 8.0) and 10mM EDTA
Storage Temp:
Store at 25°C, for 6 months or 4°C for a year. For long-term storage, store at -20°C.
Phospholipase C-gamma2 (PLCG2) PLCG2 is an enzyme mainly expressed in immune cells (including microglia) which are involved in innate immunity. It is involved in the transmembrane transduction of immune signals that determine the fate and function of various immune cell types.
PLCG2 has higher expression levels in pathologically afected brain regions in Alzheimers disease indicating that the immune system may play a key role in the development of Alzheimers disease and a polymorphism in phospholipase C-gamma 2 (PLCG2) has been reported to be protective against late onset Alzheimers disease (LOAD) [1].
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal Antibody
Format:
50 µg in 50 µl Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
Storage Temp:
Store at -20°C. Avoid freeze / thaw cycles.
Host Animal:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Expected Species:
Cat, Dog, Chimpanzee, Pig, Bovine
Immunogen:
Partial length recombinant human PLCG2 from the N-terminal region
If you would like further information regarding the immunogen used in the production of this antibody or have a query about if this antibody will bind to your protein/species please contact us and we can do the analysis for you.
[1] Sims R, van der Lee SJ, Naj AC, Bellenguez C, Badarinarayan N, Jakobsdottir J et al (2017) Rare coding variants in PLCG2, ABI3, and TREM2 implicate microglial-mediated innate immunity in Alzheimers disease. Nat Genet 49:13731384.
Phospholipase C-gamma2 (PLCG2) PLCG2 is an enzyme mainly expressed in immune cells (including microglia) which are involved in innate immunity. It is involved in the transmembrane transduction of immune signals that determine the fate and function of various immune cell types.
PLCG2 has higher expression levels in pathologically afected brain regions in Alzheimers disease indicating that the immune system may play a key role in the development of Alzheimers disease and a polymorphism in phospholipase C-gamma 2 (PLCG2) has been reported to be protective against late onset Alzheimers disease (LOAD) [1].
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal Antibody
Format:
100 µg in 100 µl Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
Storage Temp:
Store at -20°C. Avoid freeze / thaw cycles.
Host Animal:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Expected Species:
Cat, Dog, Chimpanzee, Pig, Bovine
Immunogen:
Partial length recombinant human PLCG2 from the N-terminal region
If you would like further information regarding the immunogen used in the production of this antibody or have a query about if this antibody will bind to your protein/species please contact us and we can do the analysis for you.
[1] Sims R, van der Lee SJ, Naj AC, Bellenguez C, Badarinarayan N, Jakobsdottir J et al (2017) Rare coding variants in PLCG2, ABI3, and TREM2 implicate microglial-mediated innate immunity in Alzheimers disease. Nat Genet 49:13731384.
Histamine is an important messenger molecule and neuromodulator with four known G protein-coupled receptors (GPCRs), H1, H2, H3 and H4. Histamine receptor H3 (HRH3) is a presynaptic autoreceptor that regulates histamine release from histaminergic neurons via negative feedback [1] as well as a heteroreceptor that regulates the release of other neurotransmitters [2,3,4]. It is also expressed postsynaptically in the modulation of dopamine signalling.
HRH3 is expressed predominantly in the cortex, thalamus, hypothalamus, hippocampus, amygdala and basal ganglia. Dysregulated central histaminergic signalling has been associated with a number of psychiatric disorders including sleep disorders, ADHD, Alzheimer's disease, Parkinson's disease and schizophrenia.
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal Antibody
Format:
50 µg in 50 µl Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
Storage Temp:
Store at -20°C. Avoid freeze / thaw cycles.
Host Animal:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Expected Species:
Guinae Pig, Cat
Immunogen:
A synthetic peptide from the C-terminal region of human HRH3
If you would like further information regarding the immunogen used in the production of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
Antibody Isotype:
IgG
Application Details:
WB 1:2000-5000. IHC 1:10-100.
Category:
Primary Antibodies
Other names:
HH3R, G-protein coupled receptor 97, GPCR97, H3R
Research Areas:
Neuroscience
NS Reagents Product Area:
Neuroscience
Molecular Weight:
49kDa
Subcellular location:
Plasma Membrane
Purification:
Affinity purification
References:
[1] Morisset S, Rouleau A, Ligneau X, Gbahou F, Tardivel-Lacombe J, Stark H, Schunack W, Ganellin CR, Schwartz JC, Arrang JM. High constitutive activity of native H3 receptors regulates histamine neurons in brain. Nature. 2000; 408:860-4. [2] Schlicker E, Betz R, Gothert M. Histamine H3 receptor-mediated inhibition of serotonin release in the rat brain cortex. Naunyn Schmiedebergs Arch Pharmacol. 1988; 337:588-90. [3] Schlicker E, Fink K, Hinterthaner M, Gothert M. Inhibition of noradrenaline release in the rat brain cortex via presynaptic H3 receptors. Naunyn Schmiedebergs Arch Pharmacol. 1989; 340:633-8. [4] Schlicker E, Fink K, Detzner M, Gothert M. Histamine inhibits dopamine release in the mouse striatum via presynaptic H3 receptors. J Neural Transm Gen Sect. 1993; 93:1-10.
Histamine is an important messenger molecule and neuromodulator with four known G protein-coupled receptors (GPCRs), H1, H2, H3 and H4. Histamine receptor H3 (HRH3) is a presynaptic autoreceptor that regulates histamine release from histaminergic neurons via negative feedback [1] as well as a heteroreceptor that regulates the release of other neurotransmitters [2,3,4]. It is also expressed postsynaptically in the modulation of dopamine signalling.
HRH3 is expressed predominantly in the cortex, thalamus, hypothalamus, hippocampus, amygdala and basal ganglia. Dysregulated central histaminergic signalling has been associated with a number of psychiatric disorders including sleep disorders, ADHD, Alzheimer's disease, Parkinson's disease and schizophrenia.
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal Antibody
Format:
100 µg in 100 µl Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
Storage Temp:
Store at -20°C. Avoid freeze / thaw cycles.
Host Animal:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Expected Species:
Guinae Pig, Cat
Immunogen:
A synthetic peptide from the C-terminal region of human HRH3
If you would like further information regarding the immunogen used in the production of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
[1] Morisset S, Rouleau A, Ligneau X, Gbahou F, Tardivel-Lacombe J, Stark H, Schunack W, Ganellin CR, Schwartz JC, Arrang JM. High constitutive activity of native H3 receptors regulates histamine neurons in brain. Nature. 2000; 408:860-4. [2] Schlicker E, Betz R, Gothert M. Histamine H3 receptor-mediated inhibition of serotonin release in the rat brain cortex. Naunyn Schmiedebergs Arch Pharmacol. 1988; 337:588-90. [3] Schlicker E, Fink K, Hinterthaner M, Gothert M. Inhibition of noradrenaline release in the rat brain cortex via presynaptic H3 receptors. Naunyn Schmiedebergs Arch Pharmacol. 1989; 340:633-8. [4] Schlicker E, Fink K, Detzner M, Gothert M. Histamine inhibits dopamine release in the mouse striatum via presynaptic H3 receptors. J Neural Transm Gen Sect. 1993; 93:1-10.
CD38 is a widely expressed 45 kDa transmembrane glycoprotein with a short cytoplasmic tail (aa 121), a transmembrane domain (aa 2242) and an extracellular domain (aa 43300) [1]. It is a multifunctional ectoenzyme catalysing multiple reactions and also has a role in signal transduction and calcium signaling.
In the brain CD38 is expressed in neurons, astrocytes and microglial cells and expression was found to increase under neuroinflammatory conditions indicating that it may have a role in the regulation of neuroinflammation. In Alzheimers disease, CD38 immunoreactivity is seen in intracellular tangles and neuropil threads [2].
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal Antibody
Format:
50 µg in 50 µl Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
Storage Temp:
Store at -20°C. Avoid freeze / thaw cycles.
Host Animal:
Rabbit
Species Reactivity:
Human, Rat
Immunogen:
A synthetic peptide from the C-terminal region of human CD38
If you would like further information regarding the immunogen used in the production of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
[1] Malavasi, F.; Deaglio, S.; Funaro, A.; Ferrero, E.; Horenstein, A.L.; Ortolan, E.; Vaisitti, T.; Aydin, S. Evolution and Function of the ADP Ribosyl Cyclase/CD38 Gene Family in Physiology and Pathology. Physiol. Rev. 2008, 88, 841886. [2] Otsuka K., Mizuguchi M., Aizawa T., Haga S., Sato M., Inoya H., Namba Y., Machinami R. Immunoreactivity in Alzheimers neurofibrillary tangles (abstract) Brain Pathol. 1994;4:558.
CD38 is a widely expressed 45 kDa transmembrane glycoprotein with a short cytoplasmic tail (aa 121), a transmembrane domain (aa 2242) and an extracellular domain (aa 43300) [1]. It is a multifunctional ectoenzyme catalysing multiple reactions and also has a role in signal transduction and calcium signaling.
In the brain CD38 is expressed in neurons, astrocytes and microglial cells and expression was found to increase under neuroinflammatory conditions indicating that it may have a role in the regulation of neuroinflammation. In Alzheimers disease, CD38 immunoreactivity is seen in intracellular tangles and neuropil threads [2].
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal Antibody
Format:
100 µg in 100 µl Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
Storage Temp:
Store at -20°C. Avoid freeze / thaw cycles.
Host Animal:
Rabbit
Species Reactivity:
Human, Rat
Immunogen:
A synthetic peptide from the C-terminal region of human CD38
If you would like further information regarding the immunogen used in the production of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
[1] Malavasi, F.; Deaglio, S.; Funaro, A.; Ferrero, E.; Horenstein, A.L.; Ortolan, E.; Vaisitti, T.; Aydin, S. Evolution and Function of the ADP Ribosyl Cyclase/CD38 Gene Family in Physiology and Pathology. Physiol. Rev. 2008, 88, 841886. [2] Otsuka K., Mizuguchi M., Aizawa T., Haga S., Sato M., Inoya H., Namba Y., Machinami R. Immunoreactivity in Alzheimers neurofibrillary tangles (abstract) Brain Pathol. 1994;4:558.
TREM2 (Triggering receptor expressed on myeloid cells 2) is a cell surface transmembrane glycoprotein with an immunoglobulin like extracellular domain and a cytoplasmic tail [1]. It is expressed in myeloid cells including dendritic cells, granulocytes, and tissue-specific macrophages such as osteoclasts. In the brain, TREM2 is only expressed by microglia and in the central nervous system expression of TREM2 varies with higher expression in the hippocampus, spinal cord and white matter.
TREM2 plays a complex role in neuroinflammation with expression of TREM2 being upregulated in pathological conditions such as Parkinson's disease, Amyotrophic lateral sclerosis (ALS), stroke, traumatic brain injury and Alzheimer's Disease [2]. TREM2 has also been shown to have a role in potential mechanisms linking urban air pollution to Alzheimer's Disease through its involvement in the regulation of neuroinflammation [3].
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal
Format:
50 µg in 50 µl PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
If you would like us to check if this antibody is likely to bind to this protein from a different species please contact us. We are happy to check for you.
Immunogen:
KLH conjugated synthetic peptide from the N-terminal region of human TREM2
If you would like further information regarding the immunogen used in the production of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
TREM-2, TREM2a, TREM2b, TREM2c, Trggering receptor expressed on myeloid cells 2, Trggering receptor expressed on myeloid cells 2a, Triggering receptor expressed on monocytes 2
Research Areas:
Neuroscience
NS Reagents Product Area:
Neuroscience
Molecular Weight:
25kDa (Intended as a general guide and does not allow for all isoforms and species variations)
Subcellular location:
Cell membrane, Secreted
Purification:
Affinity purification
References:
[1] Bouchon A, Dietrich J, Colonna M. Cutting edge: inflammatory responses can be triggered by TREM-1, a novel receptor expressed on neutrophils and monocytes. J Immunol. 2000;164:49915.
[2] Gratuze, M., Leyns, C.E.G. & Holtzman, D.M. New insights into the role of TREM2 in Alzheimers disease. Mol Neurodegeneration 13, 66 (2018).
[3] Hendrik J. Greve, Christen L. Mumaw, Evan J. Messenger, Prasada R. S. Kodavanti, Joyce L. Royland, Urmila P. Kodavanti and Michelle L. Block. Diesel exhaust impairs TREM2 to dysregulate neuroinflammation. J Neuroinflammation. 2020; 17: 351.
TREM2 (Triggering receptor expressed on myeloid cells 2) is a cell surface transmembrane glycoprotein with an immunoglobulin like extracellular domain and a cytoplasmic tail [1]. It is expressed in myeloid cells including dendritic cells, granulocytes, and tissue-specific macrophages such as osteoclasts. In the brain, TREM2 is only expressed by microglia and in the central nervous system expression of TREM2 varies with higher expression in the hippocampus, spinal cord and white matter.
TREM2 plays a complex role in neuroinflammation with expression of TREM2 being upregulated in pathological conditions such as Parkinson's disease, Amyotrophic lateral sclerosis (ALS), stroke, traumatic brain injury and Alzheimer's Disease [2]. TREM2 has also been shown to have a role in potential mechanisms linking urban air pollution to Alzheimer's Disease through its involvement in the regulation of neuroinflammation [3].
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal
Format:
100 µg in 100 µl PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
If you would like us to check if this antibody is likely to bind to this protein from a different species please contact us. We are happy to check for you.
Immunogen:
KLH conjugated synthetic peptide from the N-terminal region of human TREM2
If you would like further information regarding the immunogen used in the production of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
TREM-2, TREM2a, TREM2b, TREM2c, Trggering receptor expressed on myeloid cells 2, Trggering receptor expressed on myeloid cells 2a, Triggering receptor expressed on monocytes 2
Research Areas:
Neuroscience
NS Reagents Product Area:
Neuroscience
Molecular Weight:
25kDa (Intended as a general guide and does not allow for all isoforms and species variations)
Subcellular location:
Cell membrane, Secreted
Purification:
Affinity purification
References:
[1] Bouchon A, Dietrich J, Colonna M. Cutting edge: inflammatory responses can be triggered by TREM-1, a novel receptor expressed on neutrophils and monocytes. J Immunol. 2000;164:49915.
[2] Gratuze, M., Leyns, C.E.G. & Holtzman, D.M. New insights into the role of TREM2 in Alzheimers disease. Mol Neurodegeneration 13, 66 (2018).
[3] Hendrik J. Greve, Christen L. Mumaw, Evan J. Messenger, Prasada R. S. Kodavanti, Joyce L. Royland, Urmila P. Kodavanti and Michelle L. Block. Diesel exhaust impairs TREM2 to dysregulate neuroinflammation. J Neuroinflammation. 2020; 17: 351.
Lysosomal acid glucosylceramidase (GBA or Glucocerebrosidase) is the lysosomal hydrolase that hydrolyzes glucosylceramide (GC) and glucosylsphingosine (GS) to ceramide and sphingosine. It is a 536-amino-acid membrane-associated protein with a 39-amino-acid leader sequence that is cleaved to produce a 497-amino-acid mature protein.
Mutations in the GBA gene cause Gaucher disease, a lysosomal storage disease characterised by an accumulation of glucocerebrosides. Patients with Gaucher disease and heterozygous carriers are at increased risk of developing Parkinson's disease and Dementia with Lewy Bodies.
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal
Format:
50 µg in 50 µl PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
If you would like further information regarding the immunogen used in the production of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
Lysosomal acid glucosylceramidase (GBA or Glucocerebrosidase) is the lysosomal hydrolase that hydrolyzes glucosylceramide (GC) and glucosylsphingosine (GS) to ceramide and sphingosine. It is a 536-amino-acid membrane-associated protein with a 39-amino-acid leader sequence that is cleaved to produce a 497-amino-acid mature protein.
Mutations in the GBA gene cause Gaucher disease, a lysosomal storage disease characterised by an accumulation of glucocerebrosides. Patients with Gaucher disease and heterozygous carriers are at increased risk of developing Parkinson's disease and Dementia with Lewy Bodies.
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal
Format:
100 µg in 100 µl PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
If you would like further information regarding the immunogen used in the production of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
Superoxide dismutase 1 (SOD1) is one of three superoxide dismutases with SOD1 being the principal cytoplasmic superoxide dismutase in humans. It binds copper and zinc ions for its activity and stability and plays a major role in redox potential regulation. It catalyses the transformation of the superoxide anion (O2?) into hydrogen peroxide. SOD1 variants are a common cause of familial amyotrophic lateral sclerosis (ALS).
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal
Format:
50 µg in 50 µl PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
If you would like us to check if this antibody is likely to bind to this protein from a different species please contact us. We are happy to check for you.
If you would like further information regarding the immunogen used in the production of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
Antibody Isotype:
IgG
Application Details:
WB 1:500-2000. IHC 1:50-200.
Category:
Primary Antibodies
Other names:
ALS, ALS1, HEL-S-44, IPOA, SOD, hSod1, homodimer
Research Areas:
Neuroscience
NS Reagents Product Area:
Neuroscience
Molecular Weight:
16kDa (Intended as a general guide and does not allow for all isoforms and species variations)
Superoxide dismutase 1 (SOD1) is one of three superoxide dismutases with SOD1 being the principal cytoplasmic superoxide dismutase in humans. It binds copper and zinc ions for its activity and stability and plays a major role in redox potential regulation. It catalyses the transformation of the superoxide anion (O2?) into hydrogen peroxide. SOD1 variants are a common cause of familial amyotrophic lateral sclerosis (ALS).
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal
Format:
100 µg in 100 µl PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
If you would like us to check if this antibody is likely to bind to this protein from a different species please contact us. We are happy to check for you.
If you would like further information regarding the immunogen used in the production of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
Antibody Isotype:
IgG
Application Details:
WB 1:500-2000. IHC 1:50-200.
Category:
Primary Antibodies
Other names:
ALS, ALS1, HEL-S-44, IPOA, SOD, hSod1, homodimer
Research Areas:
Neuroscience
NS Reagents Product Area:
Neuroscience
Molecular Weight:
16kDa (Intended as a general guide and does not allow for all isoforms and species variations)
The ZAP-70 (zeta-associated protein of 70 kDa) tyrosine kinase was identified as a tyrosine phosphoprotein that associates with TCR zeta subunit and undergoes tyrosine phosphorylation following TCR stimulation. ZAP-70 is a Syk family tyrosine kinase primarily expressed in T and NK cells that plays an essential role in signaling through the TCR. TCR-mediated activation of T cells is crucial to the immune response. In humans, ZAP-70 gene mutations resulting in lower ZAP-70 protein expression levels or expression of catalytically inactive ZAP-70 proteins, have been identified. ZAP-70 deficiency results in the absence of mature CD8+ T cells and the prevention of TCR-mediated activation of CD4+ T cells, and it can lead to severe combined immunodeficiency.In patients with chronic lymphocytic leukemia (B-CLL), ZAP-70 expression on B cell was shown to be correlated with disease progression and survival. ZAP-70 contains two N-terminal SH2 domains (Src homology domain 2) and a C-terminal kinase domain. During T cell activation, the binding of ZAP-70 SH2 domains to the phosphorylated zeta subunit on the activated TCR complex causes a colocalization with the Lck tyrosine kinase that phosphorylates ZAP-70 on Tyr493 in the activation loop. ZAP-70 autophosphorylates multiple tyrosines in the region between the SH2 domains and the kinase domain, including the binding sites for additional SH2-containing signaling proteins such as SLP76, LAT, Lck, PLCgamma1, Vav, Shc, Ras-GAP, and Abl. ZAP-70-mediated activation of these downstream effectors leads to the release of intracellular calcium stores, and the transcription of interleukin-2 and other genes important for an immune response.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Immunogen:
A KLH-coupled peptide corresponding to amino acids 282-307 of human ZAP-70
Applications:
FC,IP,WB,ICC
Additional Info:
The mouse monoclonal antibody 1E7.2 recognizes ZAP-70, a 70 kDa protein tyrosine kinase expressed in T and NK cells.<br>_x000D_ <b>ZAP-70 is a molecule susceptible to degradation.</b> It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products.<br>_x000D_ <i>This product is for research and in vitro experimental use only. It is not to be used for any other commercial purpose. Use of this product to produce products for sale or for therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact The Regents of the Univessity of California.</i>
Clone number:
1E7.2
Antibody Isotype:
IgG1
Application Details:
Flow cytometry: Recommended dilution for purified antibody: 1 ?g/ml, intracellular staining. Recommended protocol for primary antibody conjugates using ADG Fix and Perm kit: 1) Add 50 ?l peripheral blood to a 5 ml FACS tube; 2) Proceed surface staining with appropriate amount of surface antibody (20 min at room temperature); 3) Add 100 ?l of Reagent A (Fixation Medium, stored and used at room temperature); 4) Incubate for 15 minutes at room temperature; 5) Add 4 ml PBS buffer and centrifuge at 300 g for 5 minutes at room temperature; 6) Remove supernatant and add to cells pellet 100 ?l Reagent B (Permeabilization Medium) and 20 ?l of ZAP-70-PE antibody or 10 ?l of ZAP-70-FITC antibody (red blood cells are not lysed yet); 7) Vortex at low speed for 1-2 seconds (pellet must be resuspended); 8) Incubate for 15 minutes at room temperature; 9) Add to cells mixture of 1 ml Reagent B and 3 ml PBS buffer and centrifuge at 300 g for 5 minutes at room temperature; 10) Remove supernatant and resuspend cells in 100 ?l PBS buffer; 11) Analyze immediately.Western blotting: Recommended dilution: 1-2 ?g/ml, positive control: MOLT-4 cells.
The ZAP-70 (zeta-associated protein of 70 kDa) tyrosine kinase was identified as a tyrosine phosphoprotein that associates with TCR zeta subunit and undergoes tyrosine phosphorylation following TCR stimulation. ZAP-70 is a Syk family tyrosine kinase primarily expressed in T and NK cells that plays an essential role in signaling through the TCR. TCR-mediated activation of T cells is crucial to the immune response. In humans, ZAP-70 gene mutations resulting in lower ZAP-70 protein expression levels or expression of catalytically inactive ZAP-70 proteins, have been identified. ZAP-70 deficiency results in the absence of mature CD8+ T cells and the prevention of TCR-mediated activation of CD4+ T cells, and it can lead to severe combined immunodeficiency.In patients with chronic lymphocytic leukemia (B-CLL), ZAP-70 expression on B cell was shown to be correlated with disease progression and survival. ZAP-70 contains two N-terminal SH2 domains (Src homology domain 2) and a C-terminal kinase domain. During T cell activation, the binding of ZAP-70 SH2 domains to the phosphorylated zeta subunit on the activated TCR complex causes a colocalization with the Lck tyrosine kinase that phosphorylates ZAP-70 on Tyr493 in the activation loop. ZAP-70 autophosphorylates multiple tyrosines in the region between the SH2 domains and the kinase domain, including the binding sites for additional SH2-containing signaling proteins such as SLP76, LAT, Lck, PLCgamma1, Vav, Shc, Ras-GAP, and Abl. ZAP-70-mediated activation of these downstream effectors leads to the release of intracellular calcium stores, and the transcription of interleukin-2 and other genes important for an immune response.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
A KLH-coupled peptide corresponding to amino acids 282-307 of human ZAP-70
Applications:
FC
Additional Info:
The mouse monoclonal antibody 1E7.2 recognizes ZAP-70, a 70 kDa protein tyrosine kinase expressed in T and NK cells.<br>_x000D_<b>ZAP-70 is a molecule susceptible to degradation.</b> It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products.<br>_x000D_<i>This product is for research and in vitro experimental use only. It is not to be used for any other commercial purpose. Use of this product to produce products for sale or for therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact The Regents of the Univessity of California.</i>
The ZAP-70 (zeta-associated protein of 70 kDa) tyrosine kinase was identified as a tyrosine phosphoprotein that associates with TCR zeta subunit and undergoes tyrosine phosphorylation following TCR stimulation. ZAP-70 is a Syk family tyrosine kinase primarily expressed in T and NK cells that plays an essential role in signaling through the TCR. TCR-mediated activation of T cells is crucial to the immune response. In humans, ZAP-70 gene mutations resulting in lower ZAP-70 protein expression levels or expression of catalytically inactive ZAP-70 proteins, have been identified. ZAP-70 deficiency results in the absence of mature CD8+ T cells and the prevention of TCR-mediated activation of CD4+ T cells, and it can lead to severe combined immunodeficiency.In patients with chronic lymphocytic leukemia (B-CLL), ZAP-70 expression on B cell was shown to be correlated with disease progression and survival. ZAP-70 contains two N-terminal SH2 domains (Src homology domain 2) and a C-terminal kinase domain. During T cell activation, the binding of ZAP-70 SH2 domains to the phosphorylated zeta subunit on the activated TCR complex causes a colocalization with the Lck tyrosine kinase that phosphorylates ZAP-70 on Tyr493 in the activation loop. ZAP-70 autophosphorylates multiple tyrosines in the region between the SH2 domains and the kinase domain, including the binding sites for additional SH2-containing signaling proteins such as SLP76, LAT, Lck, PLCgamma1, Vav, Shc, Ras-GAP, and Abl. ZAP-70-mediated activation of these downstream effectors leads to the release of intracellular calcium stores, and the transcription of interleukin-2 and other genes important for an immune response.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
A KLH-coupled peptide corresponding to amino acids 282-307 of human ZAP-70
Applications:
FC,IP,WB,ICC
Additional Info:
The mouse monoclonal antibody 1E7.2 recognizes ZAP-70, a 70 kDa protein tyrosine kinase expressed in T and NK cells.<br>_x000D_ <b>ZAP-70 is a molecule susceptible to degradation.</b> It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products.<br>_x000D_ <i>This product is for research and in vitro experimental use only. It is not to be used for any other commercial purpose. Use of this product to produce products for sale or for therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact The Regents of the Univessity of California.</i>
s Western Blotting Filter Paper is pre-cut cotton fiber for wet or semi-dry, passive or electrophoretic transfer of proteins from polyacrylamide gels (SDS-PAGE) to PVDF, nitrocellulose, or other membranes. These filter papers are manufactured with ultrapure water and contain no additives that can interfere with any application. The smooth sheets are suitable for use with alcohol and other organic solvents involved in protein transfer and nucleic acid blotting. The papers also provide a uniform flow of buffer through the gel to the transfer membrance in a blotting sandwich.
s Western Blotting Filter Paper is pre-cut cotton fiber for wet or semi-dry, passive or electrophoretic transfer of proteins from polyacrylamide gels (SDS-PAGE) to PVDF, nitrocellulose, or other membranes. These filter papers are manufactured with ultrapure water and contain no additives that can interfere with any application. The smooth sheets are suitable for use with alcohol and other organic solvents involved in protein transfer and nucleic acid blotting. The papers also provide a uniform flow of buffer through the gel to the transfer membrance in a blotting sandwich.
SDS PAGE Sample Buffer 2X (Reducing) is the most commonly used sample buffer for Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis (SDS-PAGE) of denatured proteins in the Laemmli SDS-PAGE system.
RIPA Lysis Buffer reagent is a complete cell lysis reagent popularly used for cultured mammalian cells. RIPA lysis buffer is highly compatible with immunoassays, protein purification procedures, immunoprecipitation, and western blotting.
Product Type:
Buffers & Mixes
Storage Temp:
Upon receipt store at 4°C. RIPA Lysis Buffer is stable for one year. Product is shipped on ice.
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